Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61516.t1 | XP_003609339.2 | 77.2 | 149 | 1 | 3 | 1 | 117 | 29 | 176 | 6.10E-46 | 193.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61516.t1 | G7JD04 | 77.2 | 149 | 1 | 3 | 1 | 117 | 1 | 148 | 4.4e-46 | 193.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051019 | MS.gene61516 | 0.80504 | 1.62E-49 | -1.69E-46 |
| MS.gene051121 | MS.gene61516 | 0.800445 | 1.44E-48 | -1.69E-46 |
| MS.gene051538 | MS.gene61516 | 0.808907 | 2.46E-50 | -1.69E-46 |
| MS.gene052579 | MS.gene61516 | 0.821561 | 3.78E-53 | -1.69E-46 |
| MS.gene055204 | MS.gene61516 | 0.817866 | 2.64E-52 | -1.69E-46 |
| MS.gene057148 | MS.gene61516 | -0.826114 | 3.24E-54 | -1.69E-46 |
| MS.gene058095 | MS.gene61516 | 0.80677 | 7.00E-50 | -1.69E-46 |
| MS.gene060338 | MS.gene61516 | 0.813017 | 3.17E-51 | -1.69E-46 |
| MS.gene060522 | MS.gene61516 | 0.844882 | 5.74E-59 | -1.69E-46 |
| MS.gene061188 | MS.gene61516 | 0.800425 | 1.45E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61516.t1 | MTR_4g114600 | 74.497 | 149 | 5 | 3 | 1 | 117 | 1 | 148 | 2.42e-66 | 197 |
| MS.gene61516.t1 | MTR_2g103750 | 35.556 | 135 | 62 | 6 | 1 | 112 | 1 | 133 | 2.29e-12 | 59.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 17 sgRNAs with CRISPR-Local
Find 31 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TACAAAGCTGCTCTGTTTGC+AGG | 0.285163 | 8.3:+13066789 | None:intergenic |
| AACAGAGCAGCTTTGTATAA+TGG | 0.334899 | 8.3:-13066783 | MS.gene61516:CDS |
| GAAATTGAATTCAAGATCAA+AGG | 0.482344 | 8.3:-13066853 | MS.gene61516:CDS |
| AAGGTTCAAAAGAATTGTAA+TGG | 0.522701 | 8.3:-13066834 | MS.gene61516:CDS |
| GTTGTGGATTCATGTAAAGA+TGG | 0.540155 | 8.3:-13066939 | MS.gene61516:CDS |
| GCAACCGCCATGGCTGTCTC+CGG | 0.543566 | 8.3:-13067212 | MS.gene61516:CDS |
| AGATCGTGTGAATCAACGAT+TGG | 0.545757 | 8.3:-13066751 | MS.gene61516:CDS |
| GTTCAAAAGAATTGTAATGG+TGG | 0.561934 | 8.3:-13066831 | MS.gene61516:CDS |
| TGCTGTCTTTGCAACCGCCA+TGG | 0.563392 | 8.3:-13067222 | MS.gene61516:CDS |
| AGCAAGGATGATGACTGTGC+CGG | 0.571426 | 8.3:+13067193 | None:intergenic |
| ATTTCTGAAGACGGAGAGCA+AGG | 0.583155 | 8.3:+13067177 | None:intergenic |
| GACTGTGCCGGAGACAGCCA+TGG | 0.611754 | 8.3:+13067205 | None:intergenic |
| TGCAAAGACAGCACCTTGTG+AGG | 0.629596 | 8.3:+13067232 | None:intergenic |
| TCTTCAATGATTACCTCACA+AGG | 0.650992 | 8.3:-13067245 | MS.gene61516:CDS |
| AATGGTGGAAACGAGAACAG+AGG | 0.717333 | 8.3:-13066816 | MS.gene61516:CDS |
| GAGTACAGAAAACAACAACA+TGG | 0.720965 | 8.3:-13066912 | MS.gene61516:CDS |
| TGTGCCGGAGACAGCCATGG+CGG | 0.736789 | 8.3:+13067208 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTTTGTTAACATAATTCT+TGG | - | chr8.3:13066965-13066984 | MS.gene61516:CDS | 15.0% |
| ! | AAGGTTCAAAAGAATTGTAA+TGG | - | chr8.3:13067154-13067173 | MS.gene61516:intron | 25.0% |
| ! | GAAATTGAATTCAAGATCAA+AGG | - | chr8.3:13067135-13067154 | MS.gene61516:intron | 25.0% |
| !! | AACAAGGTTTTTCTCTTTTT+AGG | - | chr8.3:13066937-13066956 | MS.gene61516:CDS | 25.0% |
| !! | ACAAGGTTTTTCTCTTTTTA+GGG | - | chr8.3:13066938-13066957 | MS.gene61516:CDS | 25.0% |
| !!! | TTCTTTGTTTTCTTTTCATC+TGG | + | chr8.3:13066998-13067017 | None:intergenic | 25.0% |
| AAGAAGAAGAAGAAGAAACA+TGG | + | chr8.3:13066853-13066872 | None:intergenic | 30.0% | |
| AGTAAAAAGTAACTTACCAG+AGG | + | chr8.3:13066903-13066922 | None:intergenic | 30.0% | |
| GATAAACAAGAACGTAGAAT+AGG | + | chr8.3:13066881-13066900 | None:intergenic | 30.0% | |
| GTTCAAAAGAATTGTAATGG+TGG | - | chr8.3:13067157-13067176 | MS.gene61516:intron | 30.0% | |
| !!! | GAAAAGAGTTAGTTTTGCTA+AGG | - | chr8.3:13067024-13067043 | MS.gene61516:intron | 30.0% |
| AAACATGGTGAAGAGAGAAT+TGG | + | chr8.3:13066838-13066857 | None:intergenic | 35.0% | |
| AACATGGTGAAGAGAGAATT+GGG | + | chr8.3:13066837-13066856 | None:intergenic | 35.0% | |
| CTACACACATTGAACAAACA+AGG | - | chr8.3:13066921-13066940 | MS.gene61516:CDS | 35.0% | |
| GAGTACAGAAAACAACAACA+TGG | - | chr8.3:13067076-13067095 | MS.gene61516:intron | 35.0% | |
| GTTGTGGATTCATGTAAAGA+TGG | - | chr8.3:13067049-13067068 | MS.gene61516:intron | 35.0% | |
| TCTTCAATGATTACCTCACA+AGG | - | chr8.3:13066743-13066762 | MS.gene61516:CDS | 35.0% | |
| ! | AACAGAGCAGCTTTGTATAA+TGG | - | chr8.3:13067205-13067224 | MS.gene61516:CDS | 35.0% |
| ! | AGAATTGGGATTTCTGAAGA+CGG | + | chr8.3:13066823-13066842 | None:intergenic | 35.0% |
| !! | TAGTTTTGCTAAGGATGTTG+TGG | - | chr8.3:13067033-13067052 | MS.gene61516:intron | 35.0% |
| AGATCGTGTGAATCAACGAT+TGG | - | chr8.3:13067237-13067256 | MS.gene61516:CDS | 40.0% | |
| ! | CTACGTTCTTGTTTATCCTC+TGG | - | chr8.3:13066884-13066903 | MS.gene61516:CDS | 40.0% |
| TACAAAGCTGCTCTGTTTGC+AGG | + | chr8.3:13067202-13067221 | None:intergenic | 45.0% | |
| ! | AATGGTGGAAACGAGAACAG+AGG | - | chr8.3:13067172-13067191 | MS.gene61516:intron | 45.0% |
| ! | ATTTCTGAAGACGGAGAGCA+AGG | + | chr8.3:13066814-13066833 | None:intergenic | 45.0% |
| AGCAAGGATGATGACTGTGC+CGG | + | chr8.3:13066798-13066817 | None:intergenic | 50.0% | |
| TGCAAAGACAGCACCTTGTG+AGG | + | chr8.3:13066759-13066778 | None:intergenic | 50.0% | |
| TGCTGTCTTTGCAACCGCCA+TGG | - | chr8.3:13066766-13066785 | MS.gene61516:CDS | 55.0% | |
| GACTGTGCCGGAGACAGCCA+TGG | + | chr8.3:13066786-13066805 | None:intergenic | 65.0% | |
| GCAACCGCCATGGCTGTCTC+CGG | - | chr8.3:13066776-13066795 | MS.gene61516:CDS | 65.0% | |
| TGTGCCGGAGACAGCCATGG+CGG | + | chr8.3:13066783-13066802 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 13066740 | 13067270 | 13066740 | ID=MS.gene61516 |
| chr8.3 | mRNA | 13066740 | 13067270 | 13066740 | ID=MS.gene61516.t1;Parent=MS.gene61516 |
| chr8.3 | exon | 13067189 | 13067270 | 13067189 | ID=MS.gene61516.t1.exon1;Parent=MS.gene61516.t1 |
| chr8.3 | CDS | 13067189 | 13067270 | 13067189 | ID=cds.MS.gene61516.t1;Parent=MS.gene61516.t1 |
| chr8.3 | exon | 13066740 | 13067011 | 13066740 | ID=MS.gene61516.t1.exon2;Parent=MS.gene61516.t1 |
| chr8.3 | CDS | 13066740 | 13067011 | 13066740 | ID=cds.MS.gene61516.t1;Parent=MS.gene61516.t1 |
| Gene Sequence |
| Protein sequence |
