Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61627.t1 | XP_013455695.1 | 100 | 159 | 0 | 0 | 1 | 159 | 285 | 443 | 1.60E-81 | 312 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61627.t1 | Q8RWC5 | 80.4 | 163 | 25 | 1 | 1 | 156 | 273 | 435 | 2.8e-66 | 252.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61627.t1 | A0A072UJX8 | 100.0 | 159 | 0 | 0 | 1 | 159 | 285 | 443 | 1.2e-81 | 312.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049711 | MS.gene61627 | 0.810149 | 1.33E-50 | -1.69E-46 |
| MS.gene050649 | MS.gene61627 | 0.808826 | 2.56E-50 | -1.69E-46 |
| MS.gene050657 | MS.gene61627 | 0.804522 | 2.07E-49 | -1.69E-46 |
| MS.gene051021 | MS.gene61627 | 0.833374 | 5.53E-56 | -1.69E-46 |
| MS.gene052732 | MS.gene61627 | 0.835224 | 1.90E-56 | -1.69E-46 |
| MS.gene053144 | MS.gene61627 | 0.882672 | 8.56E-71 | -1.69E-46 |
| MS.gene054584 | MS.gene61627 | 0.883979 | 2.84E-71 | -1.69E-46 |
| MS.gene055537 | MS.gene61627 | 0.818588 | 1.81E-52 | -1.69E-46 |
| MS.gene058206 | MS.gene61627 | 0.818238 | 2.18E-52 | -1.69E-46 |
| MS.gene060884 | MS.gene61627 | 0.823506 | 1.34E-53 | -1.69E-46 |
| MS.gene060886 | MS.gene61627 | 0.825305 | 5.04E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61627.t1 | MTR_4g050170 | 100.000 | 159 | 0 | 0 | 1 | 159 | 285 | 443 | 4.83e-113 | 328 |
| MS.gene61627.t1 | MTR_7g106760 | 81.366 | 161 | 26 | 2 | 1 | 159 | 271 | 429 | 4.17e-87 | 262 |
| MS.gene61627.t1 | MTR_2g087810 | 80.124 | 161 | 30 | 1 | 1 | 159 | 262 | 422 | 3.93e-86 | 259 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61627.t1 | AT2G35860 | 80.368 | 163 | 25 | 1 | 1 | 156 | 273 | 435 | 3.64e-89 | 267 |
| MS.gene61627.t1 | AT3G52370 | 80.864 | 162 | 23 | 2 | 1 | 155 | 265 | 425 | 2.78e-88 | 265 |
| MS.gene61627.t1 | AT3G52370 | 80.864 | 162 | 23 | 2 | 1 | 155 | 318 | 478 | 4.06e-88 | 266 |
| MS.gene61627.t1 | AT5G06390 | 79.503 | 161 | 25 | 2 | 1 | 155 | 284 | 442 | 3.61e-85 | 257 |
| MS.gene61627.t1 | AT3G11700 | 80.124 | 161 | 26 | 1 | 1 | 155 | 287 | 447 | 5.29e-83 | 252 |
| MS.gene61627.t1 | AT5G05650 | 58.696 | 92 | 34 | 1 | 41 | 132 | 2 | 89 | 7.92e-35 | 118 |
Find 53 sgRNAs with CRISPR-Local
Find 52 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGGGTTTACCCTTCCGTTCT+TGG | 0.196546 | 4.1:+47665518 | None:intergenic |
| TATAATGCTGTTAGAAGATT+TGG | 0.205388 | 4.1:-47665745 | MS.gene61627:CDS |
| TAACGAGATGGCGGATATTC+TGG | 0.301192 | 4.1:-47665953 | None:intergenic |
| GTTTGACCCGGATATTTATA+CGG | 0.315626 | 4.1:-47665623 | MS.gene61627:CDS |
| GCTGATGGGTCGGTTAAATT+CGG | 0.358905 | 4.1:-47665670 | MS.gene61627:CDS |
| TTAGCCATAGCTTCATCATT+TGG | 0.365438 | 4.1:+47665859 | None:intergenic |
| CTCCCATCCGTATAAATATC+CGG | 0.383323 | 4.1:+47665616 | None:intergenic |
| CAACGCACCTCTTCTTTGCT+TGG | 0.384525 | 4.1:+47665483 | None:intergenic |
| AAAAGAAGAGGTTGTTGATA+AGG | 0.389174 | 4.1:-47665548 | MS.gene61627:CDS |
| GGGTTTACCCTTCCGTTCTT+GGG | 0.397284 | 4.1:+47665519 | None:intergenic |
| ATTTCTGTGCAAGGGATTGA+TGG | 0.408807 | 4.1:-47665592 | MS.gene61627:CDS |
| TGATAGTACATAATTTGTTC+CGG | 0.408846 | 4.1:+47665802 | None:intergenic |
| TACTTTGAGATTGCCTCATA+AGG | 0.412712 | 4.1:-47665707 | MS.gene61627:CDS |
| GTGAACCCGGTTCACTCAAC+TGG | 0.414386 | 4.1:+47665824 | None:intergenic |
| TCTTCAGCATCAACCTTATG+AGG | 0.417041 | 4.1:+47665694 | None:intergenic |
| ATTCGGGCATGGTGATGGTT+CGG | 0.423960 | 4.1:-47665653 | MS.gene61627:CDS |
| ATAATGCTGTTAGAAGATTT+GGG | 0.425540 | 4.1:-47665744 | MS.gene61627:CDS |
| AACGGACCAGTTGAGTGAAC+CGG | 0.429059 | 4.1:-47665830 | MS.gene61627:CDS |
| TGCTTGGCCACAACCTTAGC+GGG | 0.430153 | 4.1:+47665499 | None:intergenic |
| CTCTCTTCAGTTTGATACTC+AGG | 0.448810 | 4.1:+47665772 | None:intergenic |
| GTTAAATTCGGGCATGGTGA+TGG | 0.463040 | 4.1:-47665658 | MS.gene61627:CDS |
| GATGAGTAGGTTAGTATCAG+AGG | 0.468619 | 4.1:-47665905 | MS.gene61627:CDS |
| CTGATGGGTCGGTTAAATTC+GGG | 0.490096 | 4.1:-47665669 | MS.gene61627:CDS |
| TTTCTGTGCAAGGGATTGAT+GGG | 0.500163 | 4.1:-47665591 | MS.gene61627:CDS |
| GACCCGGATATTTATACGGA+TGG | 0.509583 | 4.1:-47665619 | MS.gene61627:CDS |
| GGGTAAACCCGCTAAGGTTG+TGG | 0.515104 | 4.1:-47665506 | MS.gene61627:CDS |
| AGCACCAAATGATGAAGCTA+TGG | 0.522967 | 4.1:-47665863 | MS.gene61627:CDS |
| ATAATTTGTTCCGGTGAACC+CGG | 0.528069 | 4.1:+47665811 | None:intergenic |
| TTGCTTGGCCACAACCTTAG+CGG | 0.531460 | 4.1:+47665498 | None:intergenic |
| TTCGGCTTATTTGTTTGACC+CGG | 0.531979 | 4.1:-47665635 | MS.gene61627:CDS |
| ATGAGTAGGTTAGTATCAGA+GGG | 0.533753 | 4.1:-47665904 | MS.gene61627:CDS |
| ACCCGGATATTTATACGGAT+GGG | 0.536086 | 4.1:-47665618 | MS.gene61627:CDS |
| GTTGAGTGAACCGGGTTCAC+CGG | 0.536161 | 4.1:-47665821 | MS.gene61627:CDS |
| GGCTGTGACCCAAGAACGGA+AGG | 0.537283 | 4.1:-47665527 | MS.gene61627:CDS |
| TCCCATCCGTATAAATATCC+GGG | 0.540655 | 4.1:+47665617 | None:intergenic |
| TTAGAAGATTTGGGAAGGTG+AGG | 0.560046 | 4.1:-47665735 | MS.gene61627:CDS |
| TTCTGTGCAAGGGATTGATG+GGG | 0.568281 | 4.1:-47665590 | MS.gene61627:CDS |
| ACGGACCAGTTGAGTGAACC+GGG | 0.578352 | 4.1:-47665829 | MS.gene61627:CDS |
| ATGGGAGGATTTCTGTGCAA+GGG | 0.579676 | 4.1:-47665600 | MS.gene61627:CDS |
| TGCTGTTAGAAGATTTGGGA+AGG | 0.581041 | 4.1:-47665740 | MS.gene61627:CDS |
| ACGGAAGGGTAAACCCGCTA+AGG | 0.584103 | 4.1:-47665512 | MS.gene61627:CDS |
| GAACTTAACGTCGTTAGCGA+CGG | 0.595166 | 4.1:-47665929 | MS.gene61627:CDS |
| GGGTCGGTTAAATTCGGGCA+TGG | 0.604050 | 4.1:-47665664 | MS.gene61627:CDS |
| CGTTAGCGACGGAGATGAGT+AGG | 0.618054 | 4.1:-47665918 | MS.gene61627:CDS |
| GATGGGAGGATTTCTGTGCA+AGG | 0.618422 | 4.1:-47665601 | MS.gene61627:CDS |
| CGGATATTTATACGGATGGG+AGG | 0.627609 | 4.1:-47665615 | MS.gene61627:CDS |
| ATAAGGCTGTGACCCAAGAA+CGG | 0.632729 | 4.1:-47665531 | MS.gene61627:CDS |
| GTTGATGCTGAAGAAGCTGA+TGG | 0.639771 | 4.1:-47665685 | MS.gene61627:CDS |
| TGCTGAAGAAGCTGATGGGT+CGG | 0.664468 | 4.1:-47665680 | MS.gene61627:CDS |
| GTTGTGGCCAAGCAAAGAAG+AGG | 0.666635 | 4.1:-47665490 | None:intergenic |
| TTGATGCTGAAGAAGCTGAT+GGG | 0.675714 | 4.1:-47665684 | MS.gene61627:CDS |
| AGCTATGGCTAAGTTAACAA+CGG | 0.711535 | 4.1:-47665848 | MS.gene61627:CDS |
| GCTGTGACCCAAGAACGGAA+GGG | 0.741038 | 4.1:-47665526 | MS.gene61627:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATAATGCTGTTAGAAGATTT+GGG | - | chr4.1:47665693-47665712 | MS.gene61627:CDS | 25.0% |
| ! | TATAATGCTGTTAGAAGATT+TGG | - | chr4.1:47665692-47665711 | MS.gene61627:CDS | 25.0% |
| !!! | TGATAGTACATAATTTGTTC+CGG | + | chr4.1:47665638-47665657 | None:intergenic | 25.0% |
| AAAAGAAGAGGTTGTTGATA+AGG | - | chr4.1:47665889-47665908 | MS.gene61627:CDS | 30.0% | |
| AGCTATGGCTAAGTTAACAA+CGG | - | chr4.1:47665589-47665608 | MS.gene61627:CDS | 35.0% | |
| ATGAGTAGGTTAGTATCAGA+GGG | - | chr4.1:47665533-47665552 | MS.gene61627:CDS | 35.0% | |
| GTTTGACCCGGATATTTATA+CGG | - | chr4.1:47665814-47665833 | MS.gene61627:CDS | 35.0% | |
| TCCACATGAAGAAAAAGAAG+AGG | - | chr4.1:47665877-47665896 | MS.gene61627:CDS | 35.0% | |
| TTAGCCATAGCTTCATCATT+TGG | + | chr4.1:47665581-47665600 | None:intergenic | 35.0% | |
| ! | ACCTCTTCTTTTTCTTCATG+TGG | + | chr4.1:47665881-47665900 | None:intergenic | 35.0% |
| !! | TACTTTGAGATTGCCTCATA+AGG | - | chr4.1:47665730-47665749 | MS.gene61627:CDS | 35.0% |
| ACCCGGATATTTATACGGAT+GGG | - | chr4.1:47665819-47665838 | MS.gene61627:CDS | 40.0% | |
| AGCACCAAATGATGAAGCTA+TGG | - | chr4.1:47665574-47665593 | MS.gene61627:CDS | 40.0% | |
| ATTTCTGTGCAAGGGATTGA+TGG | - | chr4.1:47665845-47665864 | MS.gene61627:CDS | 40.0% | |
| CTCCCATCCGTATAAATATC+CGG | + | chr4.1:47665824-47665843 | None:intergenic | 40.0% | |
| CTCTCTTCAGTTTGATACTC+AGG | + | chr4.1:47665668-47665687 | None:intergenic | 40.0% | |
| GATGAGTAGGTTAGTATCAG+AGG | - | chr4.1:47665532-47665551 | MS.gene61627:CDS | 40.0% | |
| TCCCATCCGTATAAATATCC+GGG | + | chr4.1:47665823-47665842 | None:intergenic | 40.0% | |
| TCTTCAGCATCAACCTTATG+AGG | + | chr4.1:47665746-47665765 | None:intergenic | 40.0% | |
| TGCTGTTAGAAGATTTGGGA+AGG | - | chr4.1:47665697-47665716 | MS.gene61627:CDS | 40.0% | |
| TTAGAAGATTTGGGAAGGTG+AGG | - | chr4.1:47665702-47665721 | MS.gene61627:CDS | 40.0% | |
| TTCGGCTTATTTGTTTGACC+CGG | - | chr4.1:47665802-47665821 | MS.gene61627:CDS | 40.0% | |
| TTGATGCTGAAGAAGCTGAT+GGG | - | chr4.1:47665753-47665772 | MS.gene61627:CDS | 40.0% | |
| TTTCTGTGCAAGGGATTGAT+GGG | - | chr4.1:47665846-47665865 | MS.gene61627:CDS | 40.0% | |
| !! | ATAATTTGTTCCGGTGAACC+CGG | + | chr4.1:47665629-47665648 | None:intergenic | 40.0% |
| ATAAGGCTGTGACCCAAGAA+CGG | - | chr4.1:47665906-47665925 | MS.gene61627:CDS | 45.0% | |
| ATGGGAGGATTTCTGTGCAA+GGG | - | chr4.1:47665837-47665856 | MS.gene61627:CDS | 45.0% | |
| CGGATATTTATACGGATGGG+AGG | - | chr4.1:47665822-47665841 | MS.gene61627:CDS | 45.0% | |
| GAACTTAACGTCGTTAGCGA+CGG | - | chr4.1:47665508-47665527 | MS.gene61627:CDS | 45.0% | |
| GACCCGGATATTTATACGGA+TGG | - | chr4.1:47665818-47665837 | MS.gene61627:CDS | 45.0% | |
| GTTAAATTCGGGCATGGTGA+TGG | - | chr4.1:47665779-47665798 | MS.gene61627:CDS | 45.0% | |
| GTTGATGCTGAAGAAGCTGA+TGG | - | chr4.1:47665752-47665771 | MS.gene61627:CDS | 45.0% | |
| TTCTGTGCAAGGGATTGATG+GGG | - | chr4.1:47665847-47665866 | MS.gene61627:CDS | 45.0% | |
| ! | CTGATGGGTCGGTTAAATTC+GGG | - | chr4.1:47665768-47665787 | MS.gene61627:CDS | 45.0% |
| ! | GCTGATGGGTCGGTTAAATT+CGG | - | chr4.1:47665767-47665786 | MS.gene61627:CDS | 45.0% |
| AACGGACCAGTTGAGTGAAC+CGG | - | chr4.1:47665607-47665626 | MS.gene61627:CDS | 50.0% | |
| GATGGGAGGATTTCTGTGCA+AGG | - | chr4.1:47665836-47665855 | MS.gene61627:CDS | 50.0% | |
| GGGTTTACCCTTCCGTTCTT+GGG | + | chr4.1:47665921-47665940 | None:intergenic | 50.0% | |
| TTGCTTGGCCACAACCTTAG+CGG | + | chr4.1:47665942-47665961 | None:intergenic | 50.0% | |
| ! | TGCTGAAGAAGCTGATGGGT+CGG | - | chr4.1:47665757-47665776 | MS.gene61627:CDS | 50.0% |
| !! | ATTCGGGCATGGTGATGGTT+CGG | - | chr4.1:47665784-47665803 | MS.gene61627:CDS | 50.0% |
| ACGGAAGGGTAAACCCGCTA+AGG | - | chr4.1:47665925-47665944 | MS.gene61627:CDS | 55.0% | |
| ACGGACCAGTTGAGTGAACC+GGG | - | chr4.1:47665608-47665627 | MS.gene61627:CDS | 55.0% | |
| CGGGTTTACCCTTCCGTTCT+TGG | + | chr4.1:47665922-47665941 | None:intergenic | 55.0% | |
| CGTTAGCGACGGAGATGAGT+AGG | - | chr4.1:47665519-47665538 | MS.gene61627:CDS | 55.0% | |
| GCTGTGACCCAAGAACGGAA+GGG | - | chr4.1:47665911-47665930 | MS.gene61627:CDS | 55.0% | |
| GGGTAAACCCGCTAAGGTTG+TGG | - | chr4.1:47665931-47665950 | MS.gene61627:CDS | 55.0% | |
| GGGTCGGTTAAATTCGGGCA+TGG | - | chr4.1:47665773-47665792 | MS.gene61627:CDS | 55.0% | |
| GTGAACCCGGTTCACTCAAC+TGG | + | chr4.1:47665616-47665635 | None:intergenic | 55.0% | |
| GTTGAGTGAACCGGGTTCAC+CGG | - | chr4.1:47665616-47665635 | MS.gene61627:CDS | 55.0% | |
| TGCTTGGCCACAACCTTAGC+GGG | + | chr4.1:47665941-47665960 | None:intergenic | 55.0% | |
| GGCTGTGACCCAAGAACGGA+AGG | - | chr4.1:47665910-47665929 | MS.gene61627:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 47665491 | 47665968 | 47665491 | ID=MS.gene61627 |
| chr4.1 | mRNA | 47665491 | 47665968 | 47665491 | ID=MS.gene61627.t1;Parent=MS.gene61627 |
| chr4.1 | exon | 47665491 | 47665968 | 47665491 | ID=MS.gene61627.t1.exon1;Parent=MS.gene61627.t1 |
| chr4.1 | CDS | 47665491 | 47665968 | 47665491 | ID=cds.MS.gene61627.t1;Parent=MS.gene61627.t1 |
| Gene Sequence |
| Protein sequence |
