AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene61741


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene61741.t1 MTR_6g047570 84.783 230 21 2 1 218 82 309 1.21e-136 386
MS.gene61741.t1 MTR_0250s0040 74.208 221 50 3 2 218 21 238 1.30e-111 320
MS.gene61741.t1 MTR_0011s0260 63.374 243 59 3 1 218 96 333 1.83e-97 288
MS.gene61741.t1 MTR_0011s0210 62.140 243 62 3 1 218 96 333 1.45e-94 280
MS.gene61741.t1 MTR_4g066460 61.570 242 51 4 6 218 74 302 3.76e-89 266
MS.gene61741.t1 MTR_4g067010 62.115 227 57 6 6 218 83 294 3.34e-87 261
MS.gene61741.t1 MTR_4g066380 64.035 228 57 5 6 218 82 299 3.95e-80 243
MS.gene61741.t1 MTR_6g047550 79.487 156 15 4 1 141 94 247 1.32e-71 219
MS.gene61741.t1 MTR_0246s0020 52.830 265 76 4 1 218 90 352 6.21e-68 213
MS.gene61741.t1 MTR_0246s0020 52.830 265 76 4 1 218 90 352 8.08e-68 213
MS.gene61741.t1 MTR_0246s0050 53.097 226 85 6 3 218 80 294 1.13e-63 200
MS.gene61741.t1 MTR_4g098035 53.097 226 85 6 3 218 101 315 1.80e-63 201
MS.gene61741.t1 MTR_5g014520 53.179 173 80 1 46 218 136 307 4.56e-51 168
MS.gene61741.t1 MTR_5g014560 54.706 170 69 3 53 218 116 281 6.50e-51 167
MS.gene61741.t1 MTR_4g009540 40.000 215 115 4 18 218 94 308 2.09e-42 145
MS.gene61741.t1 MTR_4g009540 40.000 215 115 4 18 218 94 308 2.66e-42 145
MS.gene61741.t1 MTR_5g014600 41.176 221 108 6 12 218 100 312 6.93e-41 142
MS.gene61741.t1 MTR_4g092700 44.385 187 101 3 33 218 124 308 1.29e-38 136
MS.gene61741.t1 MTR_4g097920 46.104 154 79 2 66 218 138 288 2.02e-38 135
MS.gene61741.t1 MTR_4g097920 46.104 154 79 2 66 218 153 303 3.69e-38 135
MS.gene61741.t1 MTR_4g009110 36.986 219 120 4 18 218 109 327 1.12e-37 134
MS.gene61741.t1 MTR_4g097940 46.753 154 78 3 66 218 153 303 2.76e-37 132
MS.gene61741.t1 MTR_2g010450 42.286 175 86 4 52 218 161 328 4.96e-36 130
MS.gene61741.t1 MTR_5g014640 44.872 156 83 2 63 218 149 301 1.50e-35 128
MS.gene61741.t1 MTR_2g104490 40.541 185 101 4 40 218 104 285 2.20e-35 127
MS.gene61741.t1 MTR_2g104490 40.541 185 101 4 40 218 104 285 3.16e-35 127
MS.gene61741.t1 MTR_4g097950 45.930 172 78 4 58 218 60 227 3.68e-35 125
MS.gene61741.t1 MTR_2g104550 41.935 186 96 6 42 218 107 289 1.15e-34 125
MS.gene61741.t1 MTR_8g009020 35.616 219 116 6 23 218 84 300 4.84e-31 116
MS.gene61741.t1 MTR_8g069740 38.286 175 103 4 48 218 91 264 9.30e-31 114
MS.gene61741.t1 MTR_7g080780 39.080 174 96 3 45 218 130 293 1.42e-30 115
MS.gene61741.t1 MTR_7g080780 39.080 174 96 3 45 218 143 306 1.85e-30 115
MS.gene61741.t1 MTR_2g104560 31.628 215 123 6 14 218 2 202 1.34e-28 107
MS.gene61741.t1 MTR_5g014550 49.219 128 51 4 98 218 54 174 9.21e-27 102
MS.gene61741.t1 MTR_8g009010 33.803 213 87 5 24 218 73 249 1.24e-25 102
MS.gene61741.t1 MTR_2g104650 39.000 200 99 6 31 218 58 246 1.75e-25 100
MS.gene61741.t1 MTR_2g104500 34.742 213 119 6 17 218 46 249 1.08e-23 95.9
MS.gene61741.t1 MTR_2g104530 37.333 150 90 1 69 218 106 251 6.17e-23 94.0
MS.gene61741.t1 MTR_2g104590 31.050 219 122 6 6 218 6 201 1.13e-20 87.0
MS.gene61741.t1 MTR_1g072320 33.103 145 83 3 68 201 470 611 2.52e-17 80.5
MS.gene61741.t1 MTR_8g067280 34.868 152 80 4 68 204 462 609 3.60e-16 77.0
MS.gene61741.t1 MTR_8g024790 30.405 148 84 5 61 197 284 423 1.92e-15 74.7
MS.gene61741.t1 MTR_4g009510 33.108 148 73 6 79 218 53 182 3.17e-14 68.9
MS.gene61741.t1 MTR_5g030430 29.054 148 94 1 68 204 493 640 3.77e-14 71.2
MS.gene61741.t1 MTR_8g064700 26.471 204 133 7 24 217 202 398 5.16e-14 70.5
MS.gene61741.t1 MTR_4g009420 32.812 128 68 3 91 218 65 174 5.67e-14 68.2
MS.gene61741.t1 MTR_8g027495 50.000 64 32 0 68 131 458 521 1.03e-13 69.7
MS.gene61741.t1 MTR_7g451650 32.593 135 67 4 91 218 57 174 1.07e-13 67.4
MS.gene61741.t1 MTR_4g009420 32.812 128 68 3 91 218 65 174 1.15e-13 67.4
MS.gene61741.t1 MTR_7g451650 32.593 135 67 4 91 218 57 174 1.49e-13 67.0
MS.gene61741.t1 MTR_8g098275 41.250 80 47 0 52 131 385 464 1.58e-13 69.3
MS.gene61741.t1 MTR_8g098275 41.250 80 47 0 52 131 292 371 1.67e-13 68.9
MS.gene61741.t1 MTR_8g098275 41.250 80 47 0 52 131 228 307 1.87e-13 68.9
MS.gene61741.t1 MTR_7g117670 46.667 75 36 2 68 142 402 472 4.17e-13 68.2
MS.gene61741.t1 MTR_8g024770 31.507 146 84 5 44 177 251 392 8.97e-13 67.0
MS.gene61741.t1 MTR_3g498695 30.818 159 94 3 69 214 299 454 2.06e-12 65.9
MS.gene61741.t1 MTR_3g498695 30.818 159 94 3 69 214 299 454 2.09e-12 65.9
MS.gene61741.t1 MTR_3g030700 30.000 150 100 2 70 214 198 347 4.53e-12 64.7
MS.gene61741.t1 MTR_1g112680 31.214 173 88 7 47 189 295 466 4.70e-12 64.7
MS.gene61741.t1 MTR_7g050350 31.008 129 65 4 97 218 1 112 5.61e-12 61.6
MS.gene61741.t1 MTR_7g451650 31.008 129 65 4 97 218 1 112 5.61e-12 61.6
MS.gene61741.t1 MTR_7g096350 50.000 54 27 0 68 121 355 408 1.67e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene61741.t1 AT4G37850 42.925 212 97 6 12 218 111 303 1.14e-40 141
MS.gene61741.t1 AT4G37850 42.925 212 97 6 12 218 111 303 1.14e-40 141
MS.gene61741.t1 AT2G22750 45.349 172 86 3 53 218 111 280 1.89e-33 122
MS.gene61741.t1 AT2G22750 45.349 172 86 3 53 218 111 280 1.93e-33 122
MS.gene61741.t1 AT2G22750 45.349 172 86 3 53 218 139 308 2.50e-33 122
MS.gene61741.t1 AT2G22750 45.349 172 86 3 53 218 139 308 2.84e-33 122
MS.gene61741.t1 AT2G22750 45.349 172 86 3 53 218 135 304 3.04e-33 122
MS.gene61741.t1 AT2G22760 40.698 172 90 4 58 218 102 272 9.46e-33 120
MS.gene61741.t1 AT2G22760 40.698 172 90 4 58 218 102 272 1.02e-32 119
MS.gene61741.t1 AT2G22770 35.160 219 117 5 17 218 87 297 1.16e-29 112
MS.gene61741.t1 AT4G17880 35.374 147 78 4 68 202 416 557 5.41e-17 79.3
MS.gene61741.t1 AT5G46760 33.557 149 82 6 68 202 415 560 3.70e-16 77.0
MS.gene61741.t1 AT1G32640 32.414 145 86 3 68 202 452 594 6.95e-15 73.2
MS.gene61741.t1 AT1G63650 32.857 140 76 4 68 189 405 544 1.42e-14 72.4
MS.gene61741.t1 AT1G63650 32.857 140 76 4 68 189 405 544 1.42e-14 72.4
MS.gene61741.t1 AT1G63650 32.857 140 76 4 68 189 405 544 1.42e-14 72.4
MS.gene61741.t1 AT5G41315 30.345 145 78 4 68 189 441 585 8.45e-14 70.1
MS.gene61741.t1 AT5G41315 30.345 145 78 4 68 189 446 590 9.05e-14 70.1
MS.gene61741.t1 AT5G41315 30.345 145 78 4 68 189 449 593 9.24e-14 70.1
MS.gene61741.t1 AT1G01260 36.842 95 57 1 68 162 433 524 3.72e-13 68.2
MS.gene61741.t1 AT1G01260 36.842 95 57 1 68 162 433 524 3.72e-13 68.2
MS.gene61741.t1 AT1G01260 36.842 95 57 1 68 162 433 524 3.72e-13 68.2
MS.gene61741.t1 AT4G09820 32.231 121 62 1 69 189 364 464 5.92e-13 67.4
MS.gene61741.t1 AT2G46510 45.714 70 35 1 68 134 395 464 8.04e-13 67.4
MS.gene61741.t1 AT2G16910 30.968 155 85 5 69 201 315 469 2.97e-12 65.5
MS.gene61741.t1 AT4G16430 44.262 61 34 0 68 128 320 380 2.29e-11 62.8
MS.gene61741.t1 AT4G00870 32.624 141 76 4 51 179 230 363 9.42e-11 60.8

Find 0 sgRNAs with CRISPR-Local

Find 46 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTCTTTTTATGAAATAATCT+TGG + 23899:492-511 MS.gene61741:intron 15.0%
!!! TAAAAAGCGTTTGAAATTTT+TGG + 23899:617-636 MS.gene61741:CDS 20.0%
!!! TAGGAAAATATATGTTGATT+CGG + 23899:781-800 MS.gene61741:CDS 20.0%
!!! TTACTTATTGTACCTTTTTA+AGG - 23899:461-480 None:intergenic 20.0%
! AACTGAAAAGATGATTTAGA+TGG - 23899:252-271 None:intergenic 25.0%
! TCTAGTTAAAATTATGGTTG+AGG + 23899:830-849 MS.gene61741:CDS 25.0%
!! AGGACTTCTAGTTAAAATTA+TGG + 23899:824-843 MS.gene61741:CDS 25.0%
AAATTATGGTTGAGGTTCAA+AGG + 23899:838-857 MS.gene61741:CDS 30.0%
AATAATAGTGTCTTACCCTT+TGG + 23899:879-898 MS.gene61741:CDS 30.0%
TACTAATTCCTCACAAAACT+TGG + 23899:311-330 MS.gene61741:CDS 30.0%
TCAGTTTCAAATTCCATCTT+TGG + 23899:266-285 MS.gene61741:CDS 30.0%
TCTACGATAGAATTTCCAAA+GGG - 23899:897-916 None:intergenic 30.0%
! TTTTGTGAGGAATTAGTAGT+GGG - 23899:309-328 None:intergenic 30.0%
!! AATTAGTAGTGGGTGAATTT+GGG - 23899:299-318 None:intergenic 30.0%
!! TGAAGAAAGTTTTGGTGAAA+AGG - 23899:226-245 None:intergenic 30.0%
!! TGTTGATTTACCATTTCAAG+TGG + 23899:746-765 MS.gene61741:CDS 30.0%
!! TTTTGTTTCCAAGTTTTGTG+AGG - 23899:322-341 None:intergenic 30.0%
!!! TATTGTACCTTTTTAAGGTC+AGG - 23899:456-475 None:intergenic 30.0%
ATTCTTGCTTCCACTTGAAA+TGG - 23899:759-778 None:intergenic 35.0%
CAAAAACCTCAAAGAGCAAA+AGG + 23899:337-356 MS.gene61741:CDS 35.0%
CGATTGCGTAAAAATGTGTT+AGG + 23899:545-564 MS.gene61741:intron 35.0%
GTCTACGATAGAATTTCCAA+AGG - 23899:898-917 None:intergenic 35.0%
TCATGGTCAAGATCATATCA+TGG + 23899:371-390 MS.gene61741:CDS 35.0%
TCTTGTTCCTGACCTTAAAA+AGG + 23899:446-465 MS.gene61741:CDS 35.0%
TTGCGTAAAAATGTGTTAGG+TGG + 23899:548-567 MS.gene61741:intron 35.0%
! GTTTTGTGAGGAATTAGTAG+TGG - 23899:310-329 None:intergenic 35.0%
!! ATTAGTAGTGGGTGAATTTG+GGG - 23899:298-317 None:intergenic 35.0%
!! GAATTAGTAGTGGGTGAATT+TGG - 23899:300-319 None:intergenic 35.0%
AAAAGGTCTCGTACTCATCA+TGG + 23899:354-373 MS.gene61741:CDS 40.0%
CAAGTGGAAGCAAGAATCTT+AGG + 23899:762-781 MS.gene61741:CDS 40.0%
! GATGGCGATGAAGAAAGTTT+TGG - 23899:234-253 None:intergenic 40.0%
CTAGAAGTCCTTTGTGCTCT+TGG - 23899:815-834 None:intergenic 45.0%
TGCAAGGGCAATGAAGTTCT+GGG - 23899:424-443 None:intergenic 45.0%
! ACGAGACCTTTTGCTCTTTG+AGG - 23899:346-365 None:intergenic 45.0%
! ATCCAATGCCAAGAGCACAA+AGG + 23899:804-823 MS.gene61741:CDS 45.0%
!! TTTGGGGTGTTGTCCAAAGA+TGG - 23899:282-301 None:intergenic 45.0%
AATGCTCTCACGAGATGACG+AGG - 23899:718-737 None:intergenic 50.0%
AGGAGTTGTCATCGTTGCAG+AGG - 23899:698-717 None:intergenic 50.0%
CTGCAAGGGCAATGAAGTTC+TGG - 23899:425-444 None:intergenic 50.0%
GTCAGGAACAAGAGCTGCAA+GGG - 23899:439-458 None:intergenic 50.0%
GTCCTTTGTGCTCTTGGCAT+TGG - 23899:809-828 None:intergenic 50.0%
!! CCTGGTTTGGTCAAGACTGT+CGG - 23899:675-694 None:intergenic 50.0%
ATCGTTGCAGAGGCCTGGTT+TGG - 23899:688-707 None:intergenic 55.0%
CCGACAGTCTTGACCAAACC+AGG + 23899:672-691 MS.gene61741:CDS 55.0%
GGTCAGGAACAAGAGCTGCA+AGG - 23899:440-459 None:intergenic 55.0%
TTGTCATCGTTGCAGAGGCC+TGG - 23899:693-712 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
23899 gene 180 935 180 ID=MS.gene61741
23899 mRNA 180 935 180 ID=MS.gene61741.t1;Parent=MS.gene61741
23899 exon 180 467 180 ID=MS.gene61741.t1.exon1;Parent=MS.gene61741.t1
23899 CDS 180 467 180 ID=cds.MS.gene61741.t1;Parent=MS.gene61741.t1
23899 exon 567 935 567 ID=MS.gene61741.t1.exon2;Parent=MS.gene61741.t1
23899 CDS 567 935 567 ID=cds.MS.gene61741.t1;Parent=MS.gene61741.t1
Gene Sequence

>MS.gene61741

ATGGAGCTTAAAAAAATTGATCATAGCAACAAAATCAATGAAACCTTTTCACCAAAACTTTCTTCATCGCCATCTAAATCATCTTTTCAGTTTCAAATTCCATCTTTGGACAACACCCCAAATTCACCCACTACTAATTCCTCACAAAACTTGGAAACAAAAACCTCAAAGAGCAAAAGGTCTCGTACTCATCATGGTCAAGATCATATCATGGCTGAGAGAAAGAGAAGAGAGAAACTCACCCAGAACTTCATTGCCCTTGCAGCTCTTGTTCCTGACCTTAAAAAGGTACAATAAGTAATTTTCTCATGTTTCTTTTTATGAAATAATCTTGGTTACTATTTTGTGTCACACTACTCACTAATCGATTGCGTAAAAATGTGTTAGGTGGACAAGTATTCTGTATTAGTTGACACTATCAAATACTTGAAAGAACTTAAAAAGCGTTTGAAATTTTTGGAAGAACAAAATGAGAAAACAAAAATAGAGTCACCGACAGTCTTGACCAAACCAGGCCTCTGCAACGATGACAACTCCTCGTCATCTCGTGAGAGCATTGATTGTGTTGTTGATTTACCATTTCAAGTGGAAGCAAGAATCTTAGGAAAATATATGTTGATTCGGATCCAATGCCAAGAGCACAAAGGACTTCTAGTTAAAATTATGGTTGAGGTTCAAAGGTTTCAACTATTTGTTGTCAATAATAGTGTCTTACCCTTTGGAAATTCTATCGTAGACATTACCATCATTGCTTAG

Protein sequence

>MS.gene61741.t1

MELKKIDHSNKINETFSPKLSSSPSKSSFQFQIPSLDNTPNSPTTNSSQNLETKTSKSKRSRTHHGQDHIMAERKRREKLTQNFIALAALVPDLKKVDKYSVLVDTIKYLKELKKRLKFLEEQNEKTKIESPTVLTKPGLCNDDNSSSSRESIDCVVDLPFQVEARILGKYMLIRIQCQEHKGLLVKIMVEVQRFQLFVVNNSVLPFGNSIVDITIIA