AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene62614

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene62614.t1 MTR_8g106900 87.234 282 20 4 1 274 1 274 3.76e-153 428
MS.gene62614.t1 MTR_7g104720 56.364 165 66 3 109 273 246 404 1.09e-54 182
MS.gene62614.t1 MTR_1g090400 48.125 160 77 3 114 273 215 368 1.45e-45 157
MS.gene62614.t1 MTR_3g017490 46.452 155 79 2 120 274 111 261 8.70e-43 147
MS.gene62614.t1 MTR_1g051180 43.827 162 84 2 118 273 203 363 2.29e-42 149
MS.gene62614.t1 MTR_7g106500 43.030 165 87 3 114 273 116 278 7.08e-42 145
MS.gene62614.t1 MTR_8g099665 46.707 167 80 3 113 273 204 367 2.29e-40 143
MS.gene62614.t1 MTR_7g010090 35.000 280 121 7 55 273 152 431 1.37e-38 140
MS.gene62614.t1 MTR_8g038440 46.753 154 75 4 123 273 105 254 7.13e-38 134
MS.gene62614.t1 MTR_5g083110 46.452 155 77 3 119 273 90 238 4.05e-37 132
MS.gene62614.t1 MTR_7g090230 42.581 155 84 4 120 273 274 424 7.65e-37 135
MS.gene62614.t1 MTR_3g465550 45.333 150 75 3 120 268 84 227 4.44e-35 126
MS.gene62614.t1 MTR_6g011640 32.653 294 118 9 60 273 103 396 6.65e-33 124
MS.gene62614.t1 MTR_5g072400 42.784 194 102 6 81 273 68 253 8.38e-32 118
MS.gene62614.t1 MTR_3g071510 39.189 148 84 2 126 273 93 234 1.34e-29 112
MS.gene62614.t1 MTR_3g030520 38.854 157 82 3 131 273 142 298 9.38e-24 98.2
MS.gene62614.t1 MTR_5g048870 37.500 96 57 1 116 208 61 156 1.84e-12 64.3
MS.gene62614.t1 MTR_7g079015 42.045 88 45 2 164 245 1 88 2.48e-11 59.7
MS.gene62614.t1 MTR_1g095960 38.776 98 51 3 168 256 32 129 9.62e-11 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene62614.t1 AT4G10630 53.869 336 91 11 1 274 1 334 2.07e-102 302
MS.gene62614.t1 AT1G32760 52.366 317 102 12 2 274 3 314 2.51e-81 248
MS.gene62614.t1 AT5G03870 44.907 216 100 3 61 274 186 384 1.11e-48 166
MS.gene62614.t1 AT1G64500 45.699 186 88 3 92 273 189 365 3.45e-46 159
MS.gene62614.t1 AT5G06470 47.273 165 79 3 114 274 79 239 3.07e-43 147
MS.gene62614.t1 AT5G39865 36.546 249 122 6 61 273 140 388 1.23e-42 150
MS.gene62614.t1 AT5G13810 41.451 193 106 4 82 274 88 273 2.11e-41 144
MS.gene62614.t1 AT5G01420 44.318 176 88 4 103 274 232 401 6.96e-39 140
MS.gene62614.t1 AT3G57070 44.231 156 81 3 120 273 264 415 2.04e-37 137
MS.gene62614.t1 AT5G58530 45.238 168 77 3 116 274 105 266 2.95e-37 133
MS.gene62614.t1 AT3G28850 42.697 178 76 4 122 273 249 426 1.02e-36 135
MS.gene62614.t1 AT2G41330 44.805 154 80 3 121 273 251 400 1.61e-36 134
MS.gene62614.t1 AT3G11773 42.857 147 77 3 128 274 11 150 1.41e-31 114
MS.gene62614.t1 AT3G11773 43.103 116 59 3 159 274 92 200 1.72e-22 92.4

Find 90 sgRNAs with CRISPR-Local

Find 102 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCCAAGAGTTAATGATTTC+AGG 0.178045 4.2:-513981 None:intergenic
TGTAGTGATTGTAATGAAAA+TGG 0.257897 4.2:+514575 MS.gene62614:CDS
TCAAAGCAGGGTTTAGGTTT+AGG 0.271658 4.2:-514088 None:intergenic
AAAACCCGGTTCGGCTCTTT+CGG 0.272734 4.2:+514112 MS.gene62614:CDS
TTTGTTGGGAGAGGTTCTTC+AGG 0.290766 4.2:+514418 MS.gene62614:CDS
GTGGAGGAGTGGGTGGATCG+AGG 0.303371 4.2:-513917 None:intergenic
GATGGTGAGTAAACAGAAAC+AGG 0.351897 4.2:+514532 MS.gene62614:CDS
CCGATCCGAAAGAGCCGAAC+CGG 0.370965 4.2:-514117 None:intergenic
CAGGGTTTAGGTTTAGGTTT+GGG 0.379436 4.2:-514082 None:intergenic
ACCACTTTCTTCTCTCCGTT+CGG 0.396019 4.2:-514158 None:intergenic
CACCTGAAATCATTAACTCT+TGG 0.404093 4.2:+513979 MS.gene62614:CDS
AGGTGCCGAACTCGTCTTCA+TGG 0.405385 4.2:-513830 None:intergenic
CGAGAAGGTGGTGGAGGAGT+GGG 0.412430 4.2:-513927 None:intergenic
TCTTTAACTTCCACCACTTA+CGG 0.416969 4.2:+513882 MS.gene62614:CDS
GGTTGTGGAAGAGGGTTTGT+TGG 0.419535 4.2:+514403 MS.gene62614:CDS
TTGTTGGGAGAGGTTCTTCA+GGG 0.419600 4.2:+514419 MS.gene62614:CDS
GTTGTGGAAGAGGGTTTGTT+GGG 0.424770 4.2:+514404 MS.gene62614:CDS
GGAAGAGGGTTTGTTGGGAG+AGG 0.425439 4.2:+514409 MS.gene62614:CDS
GCAGGGTTTAGGTTTAGGTT+TGG 0.426987 4.2:-514083 None:intergenic
GATGTTGAAGGTTGTGGAAG+AGG 0.428644 4.2:+514394 MS.gene62614:CDS
GGGAGAGAAGCGGAAGCTTT+CGG 0.431717 4.2:-514024 None:intergenic
TTCAGGTGGTTCGGAGCGGA+GGG 0.434145 4.2:-513964 None:intergenic
GTTAATGATTTCAGGTGGTT+CGG 0.436171 4.2:-513973 None:intergenic
ACCTGAAATCATTAACTCTT+GGG 0.437778 4.2:+513980 MS.gene62614:CDS
TGGAGGAGTGGGTGGATCGA+GGG 0.448293 4.2:-513916 None:intergenic
ATGTTGAAGGTTGTGGAAGA+GGG 0.449891 4.2:+514395 MS.gene62614:CDS
CATTAACTCTTGGGAACTCA+TGG 0.451980 4.2:+513989 MS.gene62614:CDS
TTTGTGAAGGGATACTATAT+TGG 0.456511 4.2:+514359 MS.gene62614:CDS
GGAGGTGTTTGTGAGGGTTG+CGG 0.462528 4.2:+514464 MS.gene62614:CDS
GTGAAACGAGAAGGTGGTGG+AGG 0.466542 4.2:-513933 None:intergenic
TTTCAGGTGGTTCGGAGCGG+AGG 0.476568 4.2:-513965 None:intergenic
GTAGTGATTGTAATGAAAAT+GGG 0.482875 4.2:+514576 MS.gene62614:CDS
CGTGACGTTTCGATGGATAG+TGG 0.483806 4.2:+514278 MS.gene62614:CDS
CCGGTTCGGCTCTTTCGGAT+CGG 0.506227 4.2:+514117 MS.gene62614:CDS
TTGAACCATGAAGACGAGTT+CGG 0.509241 4.2:+513825 MS.gene62614:CDS
TTGGAAGAAGGGGAGAGAAG+CGG 0.510913 4.2:-514034 None:intergenic
GTTGGTGGAGGTGTTTGTGA+GGG 0.511209 4.2:+514458 MS.gene62614:CDS
CTGCGAGCGTGACGTTTCGA+TGG 0.512099 4.2:+514271 MS.gene62614:CDS
GTGGGTTTAAAGAGGAACTG+AGG 0.517564 4.2:+514297 MS.gene62614:CDS
GATGGATAGTGGGTTTAAAG+AGG 0.524140 4.2:+514289 MS.gene62614:CDS
AGGTGTTCGGAGGACATTTG+AGG 0.530160 4.2:+514202 MS.gene62614:CDS
TGAAGAGATGTTGAAGGTTG+TGG 0.532420 4.2:+514388 MS.gene62614:CDS
CTCTTTCGGATCGGTTTGAG+AGG 0.536689 4.2:+514126 MS.gene62614:CDS
GTTTAGGTTTAGGTTTGGGT+CGG 0.541231 4.2:-514078 None:intergenic
AGAGTGAAACGAGAAGGTGG+TGG 0.542507 4.2:-513936 None:intergenic
GTGAAGGGATACTATATTGG+CGG 0.545463 4.2:+514362 MS.gene62614:CDS
ACGAGAAGGTGGTGGAGGAG+TGG 0.548348 4.2:-513928 None:intergenic
GAAGGTGGTGGAGGAGTGGG+TGG 0.550463 4.2:-513924 None:intergenic
GTGGATCGAGGGTGTTGAGA+AGG 0.552372 4.2:-513905 None:intergenic
TGTTGGTGGAGGTGTTTGTG+AGG 0.554629 4.2:+514457 MS.gene62614:CDS
CAACAACGTTGAGAGGTGTT+CGG 0.556960 4.2:+514189 MS.gene62614:CDS
TTGCCAAGAAAAGCTGTTGG+TGG 0.557146 4.2:+514443 MS.gene62614:CDS
AGCGGAAGCTTTCGGTGTCG+AGG 0.562057 4.2:-514016 None:intergenic
GGCACCTCAGCACTCGGTGT+CGG 0.562950 4.2:+513846 MS.gene62614:CDS
TAGCTAACTAACTACAAAGT+GGG 0.563635 4.2:-514610 None:intergenic
GGTGTTGAGAAGGCCGTAAG+TGG 0.564170 4.2:-513895 None:intergenic
TTGAGAGTGAAACGAGAAGG+TGG 0.565568 4.2:-513939 None:intergenic
CGGTGCTGAAGAGATGTTGA+AGG 0.565844 4.2:+514382 MS.gene62614:CDS
CCGCCGAGAGTGTTTGTGAA+GGG 0.568180 4.2:+514347 MS.gene62614:CDS
ATCTATACAACAACGTTGAG+AGG 0.568839 4.2:+514182 MS.gene62614:CDS
CCAAGAAAAGCTGTTGGTGG+AGG 0.569004 4.2:+514446 MS.gene62614:CDS
ATGTAACGGAAGCTGTAAGA+TGG 0.570898 4.2:+514514 MS.gene62614:CDS
GTGACGTTTCGATGGATAGT+GGG 0.575896 4.2:+514279 MS.gene62614:CDS
ACTTTCTTCTCTCCGTTCGG+CGG 0.582123 4.2:-514155 None:intergenic
GAGTTCGGCACCTCAGCACT+CGG 0.587519 4.2:+513840 MS.gene62614:CDS
TCCGCCGAGAGTGTTTGTGA+AGG 0.587875 4.2:+514346 MS.gene62614:CDS
GTTGAGAAGGCCGTAAGTGG+TGG 0.592261 4.2:-513892 None:intergenic
GAGAGGATATGTCCGCCGAA+CGG 0.594447 4.2:+514143 MS.gene62614:CDS
CGATCCGAAAGAGCCGAACC+GGG 0.602068 4.2:-514116 None:intergenic
CTTCCGTTACATCGAAAACA+AGG 0.602706 4.2:-514503 None:intergenic
GGTTTGAGAGTGAAACGAGA+AGG 0.605117 4.2:-513942 None:intergenic
TCAGGTGGTTCGGAGCGGAG+GGG 0.606340 4.2:-513963 None:intergenic
TATCCCTTCACAAACACTCT+CGG 0.606463 4.2:-514350 None:intergenic
CAAGAGTTAATGATTTCAGG+TGG 0.609204 4.2:-513978 None:intergenic
AACTCTTGGGAACTCATGGA+AGG 0.617874 4.2:+513993 MS.gene62614:CDS
CTAGCTAACTAACTACAAAG+TGG 0.619292 4.2:-514611 None:intergenic
TGGGTTTAAAGAGGAACTGA+GGG 0.619505 4.2:+514298 MS.gene62614:CDS
ATGGTGAGTAAACAGAAACA+GGG 0.621676 4.2:+514533 MS.gene62614:CDS
GGAGCTTCTGAAAGAGAAGA+CGG 0.622205 4.2:+514319 MS.gene62614:CDS
GGTTTGCCAAGAAAAGCTGT+TGG 0.627797 4.2:+514440 MS.gene62614:CDS
TGATTTCAGGTGGTTCGGAG+CGG 0.646701 4.2:-513968 None:intergenic
GCCGAACGGAGAGAAGAAAG+TGG 0.652309 4.2:+514157 MS.gene62614:CDS
AAGTTAAAGAGACGATGTGG+TGG 0.652944 4.2:-513869 None:intergenic
GTGGCCGACACCGAGTGCTG+AGG 0.659050 4.2:-513850 None:intergenic
GACATTTGAGGCTTGCAATG+CGG 0.676497 4.2:+514214 MS.gene62614:CDS
CCCTTCACAAACACTCTCGG+CGG 0.696914 4.2:-514347 None:intergenic
CAACGTTGAGAGGTGTTCGG+AGG 0.699173 4.2:+514192 MS.gene62614:CDS
TGGAAGTTAAAGAGACGATG+TGG 0.732203 4.2:-513872 None:intergenic
TGGTGAGTAAACAGAAACAG+GGG 0.736014 4.2:+514534 MS.gene62614:CDS
GCTTCTGAAAGAGAAGACGG+TGG 0.771527 4.2:+514322 MS.gene62614:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! GTAGTGATTGTAATGAAAAT+GGG + chr4.2:514576-514595 MS.gene62614:CDS 25.0%
! TGTAGTGATTGTAATGAAAA+TGG + chr4.2:514575-514594 MS.gene62614:CDS 25.0%
ACCTGAAATCATTAACTCTT+GGG + chr4.2:513980-513999 MS.gene62614:CDS 30.0%
TTTGTGAAGGGATACTATAT+TGG + chr4.2:514359-514378 MS.gene62614:CDS 30.0%
! TTTTCTTTGTTGGATGGTTT+TGG - chr4.2:514056-514075 None:intergenic 30.0%
ATCTATACAACAACGTTGAG+AGG + chr4.2:514182-514201 MS.gene62614:CDS 35.0%
ATGGTGAGTAAACAGAAACA+GGG + chr4.2:514533-514552 MS.gene62614:CDS 35.0%
CAAGAGTTAATGATTTCAGG+TGG - chr4.2:513981-514000 None:intergenic 35.0%
CACCTGAAATCATTAACTCT+TGG + chr4.2:513979-513998 MS.gene62614:CDS 35.0%
TCCCAAGAGTTAATGATTTC+AGG - chr4.2:513984-514003 None:intergenic 35.0%
! GTTAATGATTTCAGGTGGTT+CGG - chr4.2:513976-513995 None:intergenic 35.0%
! TCTTTAACTTCCACCACTTA+CGG + chr4.2:513882-513901 MS.gene62614:CDS 35.0%
!!! GTTTTTTCAAAGCAGGGTTT+AGG - chr4.2:514097-514116 None:intergenic 35.0%
!!! TTGCCTTGTTTTCGATGTAA+CGG + chr4.2:514500-514519 MS.gene62614:CDS 35.0%
AAGTTAAAGAGACGATGTGG+TGG - chr4.2:513872-513891 None:intergenic 40.0%
ATGTTGAAGGTTGTGGAAGA+GGG + chr4.2:514395-514414 MS.gene62614:CDS 40.0%
CATTAACTCTTGGGAACTCA+TGG + chr4.2:513989-514008 MS.gene62614:CDS 40.0%
CTTCCGTTACATCGAAAACA+AGG - chr4.2:514506-514525 None:intergenic 40.0%
GATGGTGAGTAAACAGAAAC+AGG + chr4.2:514532-514551 MS.gene62614:CDS 40.0%
GTGAAGGGATACTATATTGG+CGG + chr4.2:514362-514381 MS.gene62614:CDS 40.0%
TATCCCTTCACAAACACTCT+CGG - chr4.2:514353-514372 None:intergenic 40.0%
TGAAGAGATGTTGAAGGTTG+TGG + chr4.2:514388-514407 MS.gene62614:CDS 40.0%
TGGAAGTTAAAGAGACGATG+TGG - chr4.2:513875-513894 None:intergenic 40.0%
TGGGTTTAAAGAGGAACTGA+GGG + chr4.2:514298-514317 MS.gene62614:CDS 40.0%
TGGTGAGTAAACAGAAACAG+GGG + chr4.2:514534-514553 MS.gene62614:CDS 40.0%
TTGAACCATGAAGACGAGTT+CGG + chr4.2:513825-513844 MS.gene62614:CDS 40.0%
! AGAGCAGCTTTTGATGCATT+TGG + chr4.2:514239-514258 MS.gene62614:CDS 40.0%
! ATGTAACGGAAGCTGTAAGA+TGG + chr4.2:514514-514533 MS.gene62614:CDS 40.0%
! CAGGGTTTAGGTTTAGGTTT+GGG - chr4.2:514085-514104 None:intergenic 40.0%
! GTTTAGGTTTAGGTTTGGGT+CGG - chr4.2:514081-514100 None:intergenic 40.0%
! TCAAAGCAGGGTTTAGGTTT+AGG - chr4.2:514091-514110 None:intergenic 40.0%
!! AACCCTGCTTTGAAAAAACC+CGG + chr4.2:514098-514117 MS.gene62614:CDS 40.0%
!! GATGGATAGTGGGTTTAAAG+AGG + chr4.2:514289-514308 MS.gene62614:CDS 40.0%
!! TCGGTGTTTTCTTTGTTGGA+TGG - chr4.2:514062-514081 None:intergenic 40.0%
!! TGCTTTGAAAAAACCCGGTT+CGG + chr4.2:514103-514122 MS.gene62614:CDS 40.0%
!!! AACCGGGTTTTTTCAAAGCA+GGG - chr4.2:514103-514122 None:intergenic 40.0%
!!! GTTGGATGGTTTTGGAAGAA+GGG - chr4.2:514048-514067 None:intergenic 40.0%
!!! TGTTGGATGGTTTTGGAAGA+AGG - chr4.2:514049-514068 None:intergenic 40.0%
!!! TTGGATGGTTTTGGAAGAAG+GGG - chr4.2:514047-514066 None:intergenic 40.0%
AACTCTTGGGAACTCATGGA+AGG + chr4.2:513993-514012 MS.gene62614:CDS 45.0%
ACCACTTTCTTCTCTCCGTT+CGG - chr4.2:514161-514180 None:intergenic 45.0%
GATGTTGAAGGTTGTGGAAG+AGG + chr4.2:514394-514413 MS.gene62614:CDS 45.0%
GGTTTGAGAGTGAAACGAGA+AGG - chr4.2:513945-513964 None:intergenic 45.0%
GGTTTGCCAAGAAAAGCTGT+TGG + chr4.2:514440-514459 MS.gene62614:CDS 45.0%
GTGACGTTTCGATGGATAGT+GGG + chr4.2:514279-514298 MS.gene62614:CDS 45.0%
GTGGGTTTAAAGAGGAACTG+AGG + chr4.2:514297-514316 MS.gene62614:CDS 45.0%
TTGAGAGTGAAACGAGAAGG+TGG - chr4.2:513942-513961 None:intergenic 45.0%
TTGCCAAGAAAAGCTGTTGG+TGG + chr4.2:514443-514462 MS.gene62614:CDS 45.0%
TTGTTGGGAGAGGTTCTTCA+GGG + chr4.2:514419-514438 MS.gene62614:CDS 45.0%
TTTGTTGGGAGAGGTTCTTC+AGG + chr4.2:514418-514437 MS.gene62614:CDS 45.0%
! GACATTTGAGGCTTGCAATG+CGG + chr4.2:514214-514233 MS.gene62614:CDS 45.0%
! GCAGGGTTTAGGTTTAGGTT+TGG - chr4.2:514086-514105 None:intergenic 45.0%
! GGAGCTTCTGAAAGAGAAGA+CGG + chr4.2:514319-514338 MS.gene62614:CDS 45.0%
! GTTGTGGAAGAGGGTTTGTT+GGG + chr4.2:514404-514423 MS.gene62614:CDS 45.0%
!! CAACAACGTTGAGAGGTGTT+CGG + chr4.2:514189-514208 MS.gene62614:CDS 45.0%
!! TGGGTCGGTGTTTTCTTTGT+TGG - chr4.2:514066-514085 None:intergenic 45.0%
!!! GAACCGGGTTTTTTCAAAGC+AGG - chr4.2:514104-514123 None:intergenic 45.0%
ACTTTCTTCTCTCCGTTCGG+CGG - chr4.2:514158-514177 None:intergenic 50.0%
AGAGTGAAACGAGAAGGTGG+TGG - chr4.2:513939-513958 None:intergenic 50.0%
AGGTGTTCGGAGGACATTTG+AGG + chr4.2:514202-514221 MS.gene62614:CDS 50.0%
CGTGACGTTTCGATGGATAG+TGG + chr4.2:514278-514297 MS.gene62614:CDS 50.0%
GGTTGTGGAAGAGGGTTTGT+TGG + chr4.2:514403-514422 MS.gene62614:CDS 50.0%
TTGGAAGAAGGGGAGAGAAG+CGG - chr4.2:514037-514056 None:intergenic 50.0%
! CCAAGAAAAGCTGTTGGTGG+AGG + chr4.2:514446-514465 MS.gene62614:CDS 50.0%
! CCTCCACCAACAGCTTTTCT+TGG - chr4.2:514449-514468 None:intergenic 50.0%
! CGGTGCTGAAGAGATGTTGA+AGG + chr4.2:514382-514401 MS.gene62614:CDS 50.0%
! GCTTCTGAAAGAGAAGACGG+TGG + chr4.2:514322-514341 MS.gene62614:CDS 50.0%
! TGATTTCAGGTGGTTCGGAG+CGG - chr4.2:513971-513990 None:intergenic 50.0%
!! AAAACCCGGTTCGGCTCTTT+CGG + chr4.2:514112-514131 MS.gene62614:CDS 50.0%
!! CTCTTTCGGATCGGTTTGAG+AGG + chr4.2:514126-514145 MS.gene62614:CDS 50.0%
!! GTTGGTGGAGGTGTTTGTGA+GGG + chr4.2:514458-514477 MS.gene62614:CDS 50.0%
!! TGTTGGTGGAGGTGTTTGTG+AGG + chr4.2:514457-514476 MS.gene62614:CDS 50.0%
CCCTTCACAAACACTCTCGG+CGG - chr4.2:514350-514369 None:intergenic 55.0%
GAGAGGATATGTCCGCCGAA+CGG + chr4.2:514143-514162 MS.gene62614:CDS 55.0%
GCCGAACGGAGAGAAGAAAG+TGG + chr4.2:514157-514176 MS.gene62614:CDS 55.0%
GGGAGAGAAGCGGAAGCTTT+CGG - chr4.2:514027-514046 None:intergenic 55.0%
GTTGAGAAGGCCGTAAGTGG+TGG - chr4.2:513895-513914 None:intergenic 55.0%
! GGTGTTGAGAAGGCCGTAAG+TGG - chr4.2:513898-513917 None:intergenic 55.0%
! GTGAAACGAGAAGGTGGTGG+AGG - chr4.2:513936-513955 None:intergenic 55.0%
!! AGGTGCCGAACTCGTCTTCA+TGG - chr4.2:513833-513852 None:intergenic 55.0%
!! CAACGTTGAGAGGTGTTCGG+AGG + chr4.2:514192-514211 MS.gene62614:CDS 55.0%
!! CCGCCGAGAGTGTTTGTGAA+GGG + chr4.2:514347-514366 MS.gene62614:CDS 55.0%
!! GGAAGAGGGTTTGTTGGGAG+AGG + chr4.2:514409-514428 MS.gene62614:CDS 55.0%
!! GGAGGTGTTTGTGAGGGTTG+CGG + chr4.2:514464-514483 MS.gene62614:CDS 55.0%
!! GTGGATCGAGGGTGTTGAGA+AGG - chr4.2:513908-513927 None:intergenic 55.0%
!! TCCGCCGAGAGTGTTTGTGA+AGG + chr4.2:514346-514365 MS.gene62614:CDS 55.0%
CCGATCCGAAAGAGCCGAAC+CGG - chr4.2:514120-514139 None:intergenic 60.0%
CGATCCGAAAGAGCCGAACC+GGG - chr4.2:514119-514138 None:intergenic 60.0%
CTGCGAGCGTGACGTTTCGA+TGG + chr4.2:514271-514290 MS.gene62614:CDS 60.0%
GAGTTCGGCACCTCAGCACT+CGG + chr4.2:513840-513859 MS.gene62614:CDS 60.0%
TGGAGGAGTGGGTGGATCGA+GGG - chr4.2:513919-513938 None:intergenic 60.0%
! TTCAGGTGGTTCGGAGCGGA+GGG - chr4.2:513967-513986 None:intergenic 60.0%
! TTTCAGGTGGTTCGGAGCGG+AGG - chr4.2:513968-513987 None:intergenic 60.0%
!! ACGAGAAGGTGGTGGAGGAG+TGG - chr4.2:513931-513950 None:intergenic 60.0%
!! AGCGGAAGCTTTCGGTGTCG+AGG - chr4.2:514019-514038 None:intergenic 60.0%
!! CCGGTTCGGCTCTTTCGGAT+CGG + chr4.2:514117-514136 MS.gene62614:CDS 60.0%
!! CGAGAAGGTGGTGGAGGAGT+GGG - chr4.2:513930-513949 None:intergenic 60.0%
GTGGAGGAGTGGGTGGATCG+AGG - chr4.2:513920-513939 None:intergenic 65.0%
! TCAGGTGGTTCGGAGCGGAG+GGG - chr4.2:513966-513985 None:intergenic 65.0%
!! GAAGGTGGTGGAGGAGTGGG+TGG - chr4.2:513927-513946 None:intergenic 65.0%
!! GGCACCTCAGCACTCGGTGT+CGG + chr4.2:513846-513865 MS.gene62614:CDS 65.0%
! GTGGCCGACACCGAGTGCTG+AGG - chr4.2:513853-513872 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 513801 514625 513801 ID=MS.gene62614
chr4.2 mRNA 513801 514625 513801 ID=MS.gene62614.t1;Parent=MS.gene62614
chr4.2 exon 513801 514625 513801 ID=MS.gene62614.t1.exon1;Parent=MS.gene62614.t1
chr4.2 CDS 513801 514625 513801 ID=cds.MS.gene62614.t1;Parent=MS.gene62614.t1
Gene Sequence

>MS.gene62614

ATGGGTTGCGTGTCTTCACATCTGTTGAACCATGAAGACGAGTTCGGCACCTCAGCACTCGGTGTCGGCCACCACATCGTCTCTTTAACTTCCACCACTTACGGCCTTCTCAACACCCTCGATCCACCCACTCCTCCACCACCTTCTCGTTTCACTCTCAAACCCCTCCGCTCCGAACCACCTGAAATCATTAACTCTTGGGAACTCATGGAAGGCCTCGACACCGAAAGCTTCCGCTTCTCTCCCCTTCTTCCAAAACCATCCAACAAAGAAAACACCGACCCAAACCTAAACCTAAACCCTGCTTTGAAAAAACCCGGTTCGGCTCTTTCGGATCGGTTTGAGAGGATATGTCCGCCGAACGGAGAGAAGAAAGTGGTGATCTATACAACAACGTTGAGAGGTGTTCGGAGGACATTTGAGGCTTGCAATGCGGTGAGAGCAGCTTTTGATGCATTTGGTGTACAAATCTGCGAGCGTGACGTTTCGATGGATAGTGGGTTTAAAGAGGAACTGAGGGAGCTTCTGAAAGAGAAGACGGTGGTTCCGCCGAGAGTGTTTGTGAAGGGATACTATATTGGCGGTGCTGAAGAGATGTTGAAGGTTGTGGAAGAGGGTTTGTTGGGAGAGGTTCTTCAGGGTTTGCCAAGAAAAGCTGTTGGTGGAGGTGTTTGTGAGGGTTGCGGTGATATGAGATTCTTGCCTTGTTTTCGATGTAACGGAAGCTGTAAGATGGTGAGTAAACAGAAACAGGGGAACACTGTTGTTGTTAAGTGTAGTGATTGTAATGAAAATGGGTTAGTTCTGTGCCCACTTTGTAGTTAG
Protein sequence

>MS.gene62614.t1

MGCVSSHLLNHEDEFGTSALGVGHHIVSLTSTTYGLLNTLDPPTPPPPSRFTLKPLRSEPPEIINSWELMEGLDTESFRFSPLLPKPSNKENTDPNLNLNPALKKPGSALSDRFERICPPNGEKKVVIYTTTLRGVRRTFEACNAVRAAFDAFGVQICERDVSMDSGFKEELRELLKEKTVVPPRVFVKGYYIGGAEEMLKVVEEGLLGEVLQGLPRKAVGGGVCEGCGDMRFLPCFRCNGSCKMVSKQKQGNTVVVKCSDCNENGLVLCPLCS