AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene63309

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene63309.t1 MTR_3g068035 97.125 313 4 3 1 308 1 313 0.0 596
MS.gene63309.t1 MTR_1g044155 52.396 313 132 3 1 305 55 358 2.81e-79 245
MS.gene63309.t1 MTR_5g091630 52.232 224 84 2 76 299 47 247 2.13e-69 216
MS.gene63309.t1 MTR_1g079810 51.136 264 119 3 1 257 1 261 3.28e-69 218
MS.gene63309.t1 MTR_1g073860 76.974 152 35 0 74 225 92 243 1.90e-68 216
MS.gene63309.t1 MTR_8g036060 57.727 220 76 1 27 229 20 239 3.46e-67 213
MS.gene63309.t1 MTR_5g080580 47.401 327 146 6 1 302 1 326 5.60e-66 210
MS.gene63309.t1 MTR_5g011520 61.780 191 61 1 52 230 64 254 3.28e-65 208
MS.gene63309.t1 MTR_7g080980 67.516 157 45 1 85 241 66 216 1.37e-61 197
MS.gene63309.t1 MTR_4g098450 53.226 248 101 4 53 298 51 285 2.13e-60 194
MS.gene63309.t1 MTR_3g100470 44.030 268 112 10 57 308 45 290 1.04e-57 187
MS.gene63309.t1 MTR_5g066020 49.171 181 82 4 85 255 60 240 7.16e-45 153
MS.gene63309.t1 MTR_5g089600 37.423 163 91 2 72 223 73 235 9.45e-30 113
MS.gene63309.t1 MTR_6g061670 43.966 116 62 1 103 215 125 240 7.09e-24 99.4
MS.gene63309.t1 MTR_6g061670 43.966 116 62 1 103 215 130 245 7.43e-24 99.4
MS.gene63309.t1 MTR_5g010330 36.416 173 103 4 76 242 104 275 9.37e-24 99.4
MS.gene63309.t1 MTR_5g089700 36.646 161 92 3 68 219 67 226 2.91e-23 95.9
MS.gene63309.t1 MTR_1g073870 46.053 152 35 4 74 225 50 154 3.88e-23 95.5
MS.gene63309.t1 MTR_4g057487 33.929 168 99 4 68 225 11 176 4.82e-23 95.5
MS.gene63309.t1 MTR_1g061530 42.982 114 61 2 106 215 144 257 1.16e-22 97.1
MS.gene63309.t1 MTR_1g079780 42.857 112 63 1 106 216 112 223 2.32e-22 95.5
MS.gene63309.t1 MTR_1g032910 32.530 166 96 5 72 223 97 260 1.85e-21 92.4
MS.gene63309.t1 MTR_1g053685 32.515 163 101 2 72 225 81 243 2.00e-21 92.0
MS.gene63309.t1 MTR_4g057525 33.333 162 98 3 72 223 32 193 2.77e-21 90.9
MS.gene63309.t1 MTR_5g089090 34.177 158 88 2 76 222 28 180 3.78e-21 89.7
MS.gene63309.t1 MTR_7g077120 38.514 148 85 2 74 215 125 272 8.23e-20 88.6
MS.gene63309.t1 MTR_8g098390 36.496 137 83 3 95 228 158 293 9.68e-19 85.9
MS.gene63309.t1 MTR_1g076710 43.966 116 61 2 103 215 134 248 1.04e-18 85.5
MS.gene63309.t1 MTR_8g036160 36.943 157 83 3 106 258 145 289 1.14e-18 85.5
MS.gene63309.t1 MTR_7g116320 42.982 114 63 2 103 215 143 255 3.01e-18 84.3
MS.gene63309.t1 MTR_4g098460 36.486 148 77 4 76 215 57 195 1.01e-17 82.0
MS.gene63309.t1 MTR_7g116320 42.063 126 67 3 103 223 143 267 1.22e-17 81.6
MS.gene63309.t1 MTR_7g074935 43.363 113 61 1 106 215 130 242 2.20e-17 81.6
MS.gene63309.t1 MTR_8g031100 41.096 146 80 3 76 215 125 270 2.74e-17 81.6
MS.gene63309.t1 MTR_5g011680 35.115 131 71 3 106 229 145 268 9.33e-17 79.3
MS.gene63309.t1 MTR_4g098460 36.667 150 79 4 76 215 57 200 2.41e-16 78.2
MS.gene63309.t1 MTR_8g078930 35.714 126 71 3 107 223 5 129 4.24e-16 74.7
MS.gene63309.t1 MTR_4g055813 37.903 124 75 2 106 227 22 145 4.26e-16 77.4
MS.gene63309.t1 MTR_4g069110 36.634 101 64 0 70 170 28 128 6.42e-16 74.7
MS.gene63309.t1 MTR_5g011620 31.210 157 92 4 107 261 119 261 2.35e-15 77.0
MS.gene63309.t1 MTR_4g055803 39.655 116 64 2 106 215 128 243 2.87e-15 76.3
MS.gene63309.t1 MTR_4g055833 41.176 119 58 3 106 215 125 240 3.83e-15 75.5
MS.gene63309.t1 MTR_4g101990 42.982 114 61 1 106 215 147 260 1.23e-14 73.6
MS.gene63309.t1 MTR_5g011610 33.333 117 75 2 107 222 62 176 1.70e-14 73.2
MS.gene63309.t1 MTR_5g011610 36.559 93 59 0 102 194 211 303 9.18e-13 68.2
MS.gene63309.t1 MTR_6g060670 40.541 111 64 2 106 216 180 288 1.83e-14 72.8
MS.gene63309.t1 MTR_1g071850 33.113 151 73 3 84 234 38 160 2.47e-13 67.8
MS.gene63309.t1 MTR_1g038960 33.333 144 88 4 77 216 125 264 1.33e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene63309.t1 AT3G55560 52.647 340 99 10 1 308 1 310 2.89e-80 246
MS.gene63309.t1 AT4G14465 62.613 222 66 3 76 296 67 272 1.08e-75 233
MS.gene63309.t1 AT1G76500 60.541 185 63 2 52 227 49 232 3.38e-69 218
MS.gene63309.t1 AT2G42940 49.778 225 86 3 75 299 52 249 1.10e-64 204
MS.gene63309.t1 AT2G45430 56.170 235 87 6 76 302 89 315 7.73e-64 204
MS.gene63309.t1 AT3G04570 53.279 244 88 5 1 225 1 237 1.55e-63 203
MS.gene63309.t1 AT3G60870 52.423 227 86 4 76 302 59 263 2.86e-63 201
MS.gene63309.t1 AT2G35270 65.584 154 53 0 76 229 78 231 1.07e-62 200
MS.gene63309.t1 AT4G22810 70.064 157 44 1 76 229 105 261 7.21e-59 191
MS.gene63309.t1 AT4G12050 69.677 155 46 1 76 229 118 272 2.00e-58 191
MS.gene63309.t1 AT4G35390 59.296 199 79 1 27 223 14 212 3.85e-56 184
MS.gene63309.t1 AT5G49700 51.010 198 74 3 76 250 56 253 5.41e-56 182
MS.gene63309.t1 AT4G17800 66.207 145 49 0 85 229 91 235 2.34e-55 181
MS.gene63309.t1 AT1G20900 58.730 189 69 1 46 225 56 244 2.53e-53 177
MS.gene63309.t1 AT1G14490 50.877 171 75 3 66 229 14 182 1.28e-42 146
MS.gene63309.t1 AT1G14490 52.500 160 67 3 77 229 6 163 2.57e-42 145
MS.gene63309.t1 AT2G33620 43.243 148 80 3 71 215 132 278 7.41e-25 102
MS.gene63309.t1 AT2G33620 43.243 148 80 3 71 215 132 278 7.41e-25 102
MS.gene63309.t1 AT2G33620 43.243 148 80 3 71 215 132 278 7.41e-25 102
MS.gene63309.t1 AT2G33620 43.243 148 80 3 71 215 132 278 7.41e-25 102
MS.gene63309.t1 AT4G12080 44.538 119 59 2 106 219 173 289 1.12e-24 102
MS.gene63309.t1 AT4G25320 45.133 113 59 1 106 215 169 281 1.80e-23 99.8
MS.gene63309.t1 AT4G17950 42.759 145 76 3 76 215 196 338 1.96e-23 100
MS.gene63309.t1 AT5G51590 44.348 115 57 2 106 215 181 293 3.49e-23 99.0
MS.gene63309.t1 AT5G62260 38.710 124 73 1 103 223 160 283 6.05e-23 96.7
MS.gene63309.t1 AT2G45850 41.096 146 80 3 76 215 132 277 6.94e-23 97.4
MS.gene63309.t1 AT2G45850 41.096 146 80 3 76 215 132 277 6.94e-23 97.4
MS.gene63309.t1 AT1G63470 37.415 147 85 3 76 216 147 292 1.72e-22 96.7
MS.gene63309.t1 AT3G04590 42.727 110 63 0 106 215 174 283 2.90e-22 95.1
MS.gene63309.t1 AT3G04590 42.727 110 63 0 106 215 174 283 3.81e-22 96.3
MS.gene63309.t1 AT5G62260 39.655 116 67 1 103 215 160 275 7.56e-22 95.1
MS.gene63309.t1 AT5G62260 39.655 116 67 1 103 215 160 275 7.56e-22 95.1
MS.gene63309.t1 AT2G45850 40.136 147 82 3 76 216 132 278 3.39e-21 91.7
MS.gene63309.t1 AT4G00200 43.103 116 63 1 106 218 126 241 8.17e-18 82.8
MS.gene63309.t1 AT4G00200 43.103 116 63 1 106 218 126 241 8.17e-18 82.8
MS.gene63309.t1 AT4G22770 43.333 120 65 1 106 222 153 272 1.73e-17 82.0
MS.gene63309.t1 AT4G22770 43.333 120 65 1 106 222 153 272 1.73e-17 82.0
MS.gene63309.t1 AT5G46640 40.972 144 80 2 76 216 155 296 1.65e-16 79.7
MS.gene63309.t1 AT1G63480 32.051 156 94 4 66 215 125 274 1.92e-16 79.3
MS.gene63309.t1 AT1G63480 32.051 156 94 4 66 215 125 274 1.92e-16 79.3
MS.gene63309.t1 AT3G61310 36.986 146 86 2 76 215 134 279 2.12e-16 79.0
MS.gene63309.t1 AT1G63480 32.051 156 94 4 66 215 119 268 2.33e-16 79.0
MS.gene63309.t1 AT1G63480 32.051 156 94 4 66 215 119 268 2.33e-16 79.0
MS.gene63309.t1 AT1G63480 32.051 156 94 4 66 215 119 268 2.33e-16 79.0
MS.gene63309.t1 AT1G63480 32.051 156 94 4 66 215 119 268 2.33e-16 79.0
MS.gene63309.t1 AT1G63480 32.051 156 94 4 66 215 119 268 2.33e-16 79.0
MS.gene63309.t1 AT2G36560 33.043 115 72 2 106 215 108 222 9.76e-11 62.8

Find 101 sgRNAs with CRISPR-Local

Find 106 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCGAGATACTTTCGCTTTC+GGG 0.112808 3.3:-60280138 MS.gene63309:CDS
GCAGGTCCTTTGGTTGCTTC+TGG 0.205503 3.3:-60280024 MS.gene63309:CDS
GTACCATTATGCAACAAATT+TGG 0.220914 3.3:+60279775 None:intergenic
CAGGAGCAGCCGGCTGTCTA+AGG 0.232707 3.3:+60280187 None:intergenic
GTTGTTGGTGGTTCTGTTGC+AGG 0.265821 3.3:-60280042 MS.gene63309:CDS
CCTTCTCCGGCTGGAGCTAC+TGG 0.276244 3.3:-60280099 MS.gene63309:CDS
TTCTTCTCAAGGTTTGGAAT+TGG 0.279897 3.3:-60279851 MS.gene63309:CDS
GGTTATAACAATGGGTGGTT+TGG 0.288461 3.3:+60280353 None:intergenic
TTGCTGTTGCTGCTGTTTGT+TGG 0.296688 3.3:+60280489 None:intergenic
GAAGGAGGAGGACGAGGAGG+TGG 0.303163 3.3:+60279721 None:intergenic
GTTATAACAATGGGTGGTTT+GGG 0.305692 3.3:+60280354 None:intergenic
AAATGCCTCATGATGTGTTT+TGG 0.306421 3.3:-60279750 MS.gene63309:CDS
CCTCCAAATTTGTTGCATAA+TGG 0.306945 3.3:-60279778 MS.gene63309:CDS
TCTTCTCAAGGTTTGGAATT+GGG 0.310019 3.3:-60279850 MS.gene63309:CDS
GGTTGCTTCTGGACCTGTTA+TGG 0.315696 3.3:-60280013 MS.gene63309:CDS
TGTTTATTTAGCTGGTGGTC+AGG 0.322450 3.3:-60280070 MS.gene63309:CDS
GGTGGTCAGGGGCAGGTTGT+TGG 0.325929 3.3:-60280057 MS.gene63309:CDS
GGTGGTGTTATTACTCTTCA+TGG 0.329783 3.3:-60280165 MS.gene63309:CDS
ATTCGAGATACTTTCGCTTT+CGG 0.341376 3.3:-60280139 MS.gene63309:CDS
CTACCTCCGCCTTCTCCGGC+TGG 0.357947 3.3:-60280108 MS.gene63309:CDS
ACATGGCTTCTTAGCGCGTT+GGG 0.359556 3.3:+60280321 None:intergenic
GAAAAGAGAGCAAGAGTTGA+TGG 0.369566 3.3:-60280535 MS.gene63309:CDS
TAGTTCTTCTTCTCAAGGTT+TGG 0.373138 3.3:-60279857 MS.gene63309:CDS
GGATTAACTGTTTATTTAGC+TGG 0.378249 3.3:-60280078 MS.gene63309:CDS
GTAGCAGCAATAACCATAAC+AGG 0.415453 3.3:+60280000 None:intergenic
GTGGGCTGGAAATGTGGCTA+TGG 0.419505 3.3:-60280604 MS.gene63309:CDS
GGAGGAGGACGAGGAGGTGG+AGG 0.420358 3.3:+60279724 None:intergenic
TTTAGCTGGTGGTCAGGGGC+AGG 0.423340 3.3:-60280064 MS.gene63309:CDS
CTTAGACAGCCGGCTGCTCC+TGG 0.434867 3.3:-60280186 MS.gene63309:CDS
GCATCATCAACAACTTCAAC+AGG 0.441442 3.3:-60279824 MS.gene63309:CDS
TGGTTCTGTTGCAGGTCCTT+TGG 0.443762 3.3:-60280034 MS.gene63309:CDS
GGTCAGGGGCAGGTTGTTGG+TGG 0.444022 3.3:-60280054 MS.gene63309:CDS
CCACTGCTACCTGGCTCAGA+TGG 0.446968 3.3:+60280420 None:intergenic
CTCCGACCACCACTGCTACC+TGG 0.451310 3.3:+60280411 None:intergenic
CGCGCTAAGAAGCCATGTCT+TGG 0.453833 3.3:-60280316 MS.gene63309:CDS
GTTACTAGTTCTTCTTCTCA+AGG 0.454048 3.3:-60279862 MS.gene63309:CDS
ATGGCAAATCGTTGGTGGGC+TGG 0.456372 3.3:-60280618 MS.gene63309:CDS
CTAAAACTCTTTCAGAAAGA+AGG 0.460091 3.3:+60279703 None:intergenic
GACATGGCTTCTTAGCGCGT+TGG 0.461821 3.3:+60280320 None:intergenic
GTTTATTTAGCTGGTGGTCA+GGG 0.471442 3.3:-60280069 MS.gene63309:CDS
CACTGCTACCTGGCTCAGAT+GGG 0.484019 3.3:+60280421 None:intergenic
GTGACGGCGGTTAGCAAAGT+TGG 0.484913 3.3:+60280257 None:intergenic
CAGAAAGAAGGAGGAGGACG+AGG 0.494395 3.3:+60279715 None:intergenic
ACCACCTTGTTCATCTTCCA+AGG 0.497250 3.3:+60279948 None:intergenic
TTTGGAGGCAAATTATACAT+AGG 0.498673 3.3:+60279793 None:intergenic
GAAATGCAAGTTCAACAATC+TGG 0.502411 3.3:-60279913 MS.gene63309:CDS
TTCAGATGTTCAAGTGATGA+TGG 0.505534 3.3:+60280555 None:intergenic
GATTCTTTGGTTATAACAAT+GGG 0.518538 3.3:+60280345 None:intergenic
GGATTCTTTGGTTATAACAA+TGG 0.520979 3.3:+60280344 None:intergenic
TACTTATGAGAGGTTGCCCT+TGG 0.521945 3.3:-60279965 MS.gene63309:CDS
TGGGCTGGAAATGTGGCTAT+GGG 0.522347 3.3:-60280603 MS.gene63309:CDS
CCGCCGTCACCGTGGTGTGT+CGG 0.526432 3.3:-60280244 MS.gene63309:CDS
GTAGCTCCAGCCGGAGAAGG+CGG 0.528688 3.3:+60280102 None:intergenic
CCAGTAGCTCCAGCCGGAGA+AGG 0.532784 3.3:+60280099 None:intergenic
CACTGCTGATTTCCAAGACA+TGG 0.534335 3.3:+60280304 None:intergenic
GAACAAAACCCATCTGAGCC+AGG 0.536402 3.3:-60280429 MS.gene63309:CDS
AGGCCACGTGGGAGACCACC+AGG 0.537133 3.3:-60280390 MS.gene63309:CDS
GCTCCAGCCGGAGAAGGCGG+AGG 0.543492 3.3:+60280105 None:intergenic
TTAACTGTTTATTTAGCTGG+TGG 0.548179 3.3:-60280075 MS.gene63309:CDS
AAACCGACACACCACGGTGA+CGG 0.553487 3.3:+60280241 None:intergenic
CCATTATGCAACAAATTTGG+AGG 0.558327 3.3:+60279778 None:intergenic
TAATGTAACCCTTAGACAGC+CGG 0.563997 3.3:-60280196 MS.gene63309:CDS
AGATTATGGCAAATCGTTGG+TGG 0.564432 3.3:-60280623 None:intergenic
ATAACACCACCAGGAGCAGC+CGG 0.567921 3.3:+60280177 None:intergenic
TTTATTTAGCTGGTGGTCAG+GGG 0.570136 3.3:-60280068 MS.gene63309:CDS
AGGAGCAGCCGGCTGTCTAA+GGG 0.570747 3.3:+60280188 None:intergenic
CATGGCTTCTTAGCGCGTTG+GGG 0.572748 3.3:+60280322 None:intergenic
TCAAGTGATGATGGTGATGA+AGG 0.576683 3.3:+60280564 None:intergenic
AGAAGATTATGGCAAATCGT+TGG 0.586611 3.3:-60280626 None:intergenic
TTGCTAATGCTACTTATGAG+AGG 0.586891 3.3:-60279975 MS.gene63309:CDS
TCTTTGGTTATAACAATGGG+TGG 0.587176 3.3:+60280348 None:intergenic
AGCCAGGTAGCAGTGGTGGT+CGG 0.589179 3.3:-60280413 MS.gene63309:CDS
AGTGGTGGTCGGAGGCCACG+TGG 0.591936 3.3:-60280402 MS.gene63309:CDS
GCAAGTTCAACAATCTGGTG+TGG 0.593311 3.3:-60279908 MS.gene63309:CDS
ATGGCTTCTTAGCGCGTTGG+GGG 0.597936 3.3:+60280323 None:intergenic
GATTATGGCAAATCGTTGGT+GGG 0.599720 3.3:-60280622 None:intergenic
TCTGAGCCAGGTAGCAGTGG+TGG 0.601683 3.3:-60280417 MS.gene63309:CDS
CCATCTGAGCCAGGTAGCAG+TGG 0.602057 3.3:-60280420 MS.gene63309:CDS
CTCTTTCAGAAAGAAGGAGG+AGG 0.605004 3.3:+60279709 None:intergenic
AGACAGCCGGCTGCTCCTGG+TGG 0.608408 3.3:-60280183 MS.gene63309:CDS
AAAGAAGGAGGAGGACGAGG+AGG 0.613275 3.3:+60279718 None:intergenic
CCGACACACCACGGTGACGG+CGG 0.613394 3.3:+60280244 None:intergenic
CAGGTAGCAGTGGTGGTCGG+AGG 0.616953 3.3:-60280410 MS.gene63309:CDS
GTTAATCCAGTAGCTCCAGC+CGG 0.618525 3.3:+60280093 None:intergenic
ACAAGGTGGTGGTGATCAAG+AGG 0.619032 3.3:-60279935 MS.gene63309:CDS
CCACCTTGTTCATCTTCCAA+GGG 0.619149 3.3:+60279949 None:intergenic
GTGGTGGTCGGAGGCCACGT+GGG 0.622577 3.3:-60280401 MS.gene63309:CDS
TCGTTGGTGGGCTGGAAATG+TGG 0.623949 3.3:-60280610 MS.gene63309:CDS
CAGCAGTGGTAGTGATGTGG+CGG 0.628263 3.3:-60280289 MS.gene63309:CDS
CACTCAAAACCGACACACCA+CGG 0.631096 3.3:+60280235 None:intergenic
AAACTCTTTCAGAAAGAAGG+AGG 0.632824 3.3:+60279706 None:intergenic
TTTGCTAACCGCCGTCACCG+TGG 0.638394 3.3:-60280252 MS.gene63309:CDS
GGACCCCAAAACACATCATG+AGG 0.638475 3.3:+60279745 None:intergenic
TTGGAAGATGAACAAGGTGG+TGG 0.639490 3.3:-60279946 MS.gene63309:CDS
GATCCTGGTGGTCTCCCACG+TGG 0.640272 3.3:+60280387 None:intergenic
TTGCCCTTGGAAGATGAACA+AGG 0.667444 3.3:-60279952 MS.gene63309:CDS
TGAAGAGTAATAACACCACC+AGG 0.674054 3.3:+60280168 None:intergenic
CATGTCTTGGAAATCAGCAG+TGG 0.685401 3.3:-60280303 MS.gene63309:CDS
ACAGGTCCAGAAGCAACCAA+AGG 0.692630 3.3:+60280018 None:intergenic
AATCAGCAGTGGTAGTGATG+TGG 0.709338 3.3:-60280292 MS.gene63309:CDS
CCCTTGGAAGATGAACAAGG+TGG 0.848232 3.3:-60279949 MS.gene63309:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! GATTCTTTGGTTATAACAAT+GGG + chr3.3:60279990-60280009 None:intergenic 25.0%
GGATTAACTGTTTATTTAGC+TGG - chr3.3:60280254-60280273 MS.gene63309:CDS 30.0%
GGATTCTTTGGTTATAACAA+TGG + chr3.3:60279991-60280010 None:intergenic 30.0%
GTACCATTATGCAACAAATT+TGG + chr3.3:60280560-60280579 None:intergenic 30.0%
TTAACTGTTTATTTAGCTGG+TGG - chr3.3:60280257-60280276 MS.gene63309:CDS 30.0%
TTTGGAGGCAAATTATACAT+AGG + chr3.3:60280542-60280561 None:intergenic 30.0%
ATTCGAGATACTTTCGCTTT+CGG - chr3.3:60280193-60280212 MS.gene63309:CDS 35.0%
CCATTATGCAACAAATTTGG+AGG + chr3.3:60280557-60280576 None:intergenic 35.0%
GAAATGCAAGTTCAACAATC+TGG - chr3.3:60280419-60280438 MS.gene63309:CDS 35.0%
GTTATAACAATGGGTGGTTT+GGG + chr3.3:60279981-60280000 None:intergenic 35.0%
TAGTTCTTCTTCTCAAGGTT+TGG - chr3.3:60280475-60280494 MS.gene63309:CDS 35.0%
TCTTTGGTTATAACAATGGG+TGG + chr3.3:60279987-60280006 None:intergenic 35.0%
TTCAGATGTTCAAGTGATGA+TGG + chr3.3:60279780-60279799 None:intergenic 35.0%
! AAATGCCTCATGATGTGTTT+TGG - chr3.3:60280582-60280601 MS.gene63309:CDS 35.0%
! AATGCCTCATGATGTGTTTT+GGG - chr3.3:60280583-60280602 MS.gene63309:CDS 35.0%
! GTTACTAGTTCTTCTTCTCA+AGG - chr3.3:60280470-60280489 MS.gene63309:CDS 35.0%
! TCTTCTCAAGGTTTGGAATT+GGG - chr3.3:60280482-60280501 MS.gene63309:CDS 35.0%
! TTCTTCTCAAGGTTTGGAAT+TGG - chr3.3:60280481-60280500 MS.gene63309:CDS 35.0%
! TTGCTAATGCTACTTATGAG+AGG - chr3.3:60280357-60280376 MS.gene63309:CDS 35.0%
!! CCTCCAAATTTGTTGCATAA+TGG - chr3.3:60280554-60280573 MS.gene63309:CDS 35.0%
CAACAACAACAACAACGAAG+AGG - chr3.3:60279863-60279882 MS.gene63309:CDS 40.0%
GAAAAGAGAGCAAGAGTTGA+TGG - chr3.3:60279797-60279816 MS.gene63309:CDS 40.0%
GCATCATCAACAACTTCAAC+AGG - chr3.3:60280508-60280527 MS.gene63309:CDS 40.0%
GGTTATAACAATGGGTGGTT+TGG + chr3.3:60279982-60280001 None:intergenic 40.0%
GTAGCAGCAATAACCATAAC+AGG + chr3.3:60280335-60280354 None:intergenic 40.0%
GTTTATTTAGCTGGTGGTCA+GGG - chr3.3:60280263-60280282 MS.gene63309:CDS 40.0%
TAATGTAACCCTTAGACAGC+CGG - chr3.3:60280136-60280155 MS.gene63309:CDS 40.0%
TGAAGAGTAATAACACCACC+AGG + chr3.3:60280167-60280186 None:intergenic 40.0%
TGTTTATTTAGCTGGTGGTC+AGG - chr3.3:60280262-60280281 MS.gene63309:CDS 40.0%
TTCGAGATACTTTCGCTTTC+GGG - chr3.3:60280194-60280213 MS.gene63309:CDS 40.0%
TTTATTTAGCTGGTGGTCAG+GGG - chr3.3:60280264-60280283 MS.gene63309:CDS 40.0%
! ATGCCTCATGATGTGTTTTG+GGG - chr3.3:60280584-60280603 MS.gene63309:CDS 40.0%
!! GGTGGTGTTATTACTCTTCA+TGG - chr3.3:60280167-60280186 MS.gene63309:CDS 40.0%
!! TCAAGTGATGATGGTGATGA+AGG + chr3.3:60279771-60279790 None:intergenic 40.0%
!!! TTGGGTTTGTTTTTGGATCC+TGG + chr3.3:60279963-60279982 None:intergenic 40.0%
ACCACCTTGTTCATCTTCCA+AGG + chr3.3:60280387-60280406 None:intergenic 45.0%
CATGTCTTGGAAATCAGCAG+TGG - chr3.3:60280029-60280048 MS.gene63309:CDS 45.0%
CCACCTTGTTCATCTTCCAA+GGG + chr3.3:60280386-60280405 None:intergenic 45.0%
GCAAGTTCAACAATCTGGTG+TGG - chr3.3:60280424-60280443 MS.gene63309:CDS 45.0%
TACTTATGAGAGGTTGCCCT+TGG - chr3.3:60280367-60280386 MS.gene63309:CDS 45.0%
TTGCCCTTGGAAGATGAACA+AGG - chr3.3:60280380-60280399 MS.gene63309:CDS 45.0%
TTGGAAGATGAACAAGGTGG+TGG - chr3.3:60280386-60280405 MS.gene63309:CDS 45.0%
! AATCAGCAGTGGTAGTGATG+TGG - chr3.3:60280040-60280059 MS.gene63309:CDS 45.0%
! TTGCTGTTGCTGCTGTTTGT+TGG + chr3.3:60279846-60279865 None:intergenic 45.0%
!! CACTGCTGATTTCCAAGACA+TGG + chr3.3:60280031-60280050 None:intergenic 45.0%
!! GGGTGGTTTGGGTTTGTTTT+TGG + chr3.3:60279970-60279989 None:intergenic 45.0%
!!! GGTTTGTTTTTGGATCCTGG+TGG + chr3.3:60279960-60279979 None:intergenic 45.0%
ACAGGTCCAGAAGCAACCAA+AGG + chr3.3:60280317-60280336 None:intergenic 50.0%
ACATGGCTTCTTAGCGCGTT+GGG + chr3.3:60280014-60280033 None:intergenic 50.0%
CACTCAAAACCGACACACCA+CGG + chr3.3:60280100-60280119 None:intergenic 50.0%
CCCTTGGAAGATGAACAAGG+TGG - chr3.3:60280383-60280402 MS.gene63309:CDS 50.0%
GAACAAAACCCATCTGAGCC+AGG - chr3.3:60279903-60279922 MS.gene63309:CDS 50.0%
GGACCCCAAAACACATCATG+AGG + chr3.3:60280590-60280609 None:intergenic 50.0%
GGTTGCTTCTGGACCTGTTA+TGG - chr3.3:60280319-60280338 MS.gene63309:CDS 50.0%
GTTAATCCAGTAGCTCCAGC+CGG + chr3.3:60280242-60280261 None:intergenic 50.0%
! ACAAGGTGGTGGTGATCAAG+AGG - chr3.3:60280397-60280416 MS.gene63309:CDS 50.0%
! TGGGCTGGAAATGTGGCTAT+GGG - chr3.3:60279729-60279748 MS.gene63309:CDS 50.0%
! TGGTTCTGTTGCAGGTCCTT+TGG - chr3.3:60280298-60280317 MS.gene63309:CDS 50.0%
! TTTTCTACCTCCGCCTTCTC+CGG - chr3.3:60280220-60280239 MS.gene63309:CDS 50.0%
!! GTTGTTGGTGGTTCTGTTGC+AGG - chr3.3:60280290-60280309 MS.gene63309:CDS 50.0%
!!! GTGGTGTGTCGGTTTTGAGT+GGG - chr3.3:60280099-60280118 MS.gene63309:CDS 50.0%
AAACCGACACACCACGGTGA+CGG + chr3.3:60280094-60280113 None:intergenic 55.0%
AAAGAAGGAGGAGGACGAGG+AGG + chr3.3:60280617-60280636 None:intergenic 55.0%
ATAACACCACCAGGAGCAGC+CGG + chr3.3:60280158-60280177 None:intergenic 55.0%
ATGGCTTCTTAGCGCGTTGG+GGG + chr3.3:60280012-60280031 None:intergenic 55.0%
CACTGCTACCTGGCTCAGAT+GGG + chr3.3:60279914-60279933 None:intergenic 55.0%
CAGAAAGAAGGAGGAGGACG+AGG + chr3.3:60280620-60280639 None:intergenic 55.0%
CATGGCTTCTTAGCGCGTTG+GGG + chr3.3:60280013-60280032 None:intergenic 55.0%
CGCGCTAAGAAGCCATGTCT+TGG - chr3.3:60280016-60280035 MS.gene63309:CDS 55.0%
GACATGGCTTCTTAGCGCGT+TGG + chr3.3:60280015-60280034 None:intergenic 55.0%
GCAGGTCCTTTGGTTGCTTC+TGG - chr3.3:60280308-60280327 MS.gene63309:CDS 55.0%
GTGACGGCGGTTAGCAAAGT+TGG + chr3.3:60280078-60280097 None:intergenic 55.0%
TAGCGCGTTGGGGGATTCTT+TGG + chr3.3:60280003-60280022 None:intergenic 55.0%
! ATGGCAAATCGTTGGTGGGC+TGG - chr3.3:60279714-60279733 MS.gene63309:CDS 55.0%
! CAGCAGTGGTAGTGATGTGG+CGG - chr3.3:60280043-60280062 MS.gene63309:CDS 55.0%
!! GTGGGCTGGAAATGTGGCTA+TGG - chr3.3:60279728-60279747 MS.gene63309:CDS 55.0%
!! TCGTTGGTGGGCTGGAAATG+TGG - chr3.3:60279722-60279741 MS.gene63309:CDS 55.0%
!!! CGTGGTGTGTCGGTTTTGAG+TGG - chr3.3:60280098-60280117 MS.gene63309:CDS 55.0%
!!! GTGTCGGTTTTGAGTGGGAG+CGG - chr3.3:60280104-60280123 MS.gene63309:CDS 55.0%
AGCCAGGTAGCAGTGGTGGT+CGG - chr3.3:60279919-60279938 MS.gene63309:CDS 60.0%
AGGAGCAGCCGGCTGTCTAA+GGG + chr3.3:60280147-60280166 None:intergenic 60.0%
CCACTGCTACCTGGCTCAGA+TGG + chr3.3:60279915-60279934 None:intergenic 60.0%
CCATCTGAGCCAGGTAGCAG+TGG - chr3.3:60279912-60279931 MS.gene63309:CDS 60.0%
TCTGAGCCAGGTAGCAGTGG+TGG - chr3.3:60279915-60279934 MS.gene63309:CDS 60.0%
TTTAGCTGGTGGTCAGGGGC+AGG - chr3.3:60280268-60280287 MS.gene63309:CDS 60.0%
TTTGCTAACCGCCGTCACCG+TGG - chr3.3:60280080-60280099 MS.gene63309:CDS 60.0%
CAGGAGCAGCCGGCTGTCTA+AGG + chr3.3:60280148-60280167 None:intergenic 65.0%
CAGGTAGCAGTGGTGGTCGG+AGG - chr3.3:60279922-60279941 MS.gene63309:CDS 65.0%
CCAGTAGCTCCAGCCGGAGA+AGG + chr3.3:60280236-60280255 None:intergenic 65.0%
CCTTCTCCGGCTGGAGCTAC+TGG - chr3.3:60280233-60280252 MS.gene63309:CDS 65.0%
CTCCGACCACCACTGCTACC+TGG + chr3.3:60279924-60279943 None:intergenic 65.0%
CTTAGACAGCCGGCTGCTCC+TGG - chr3.3:60280146-60280165 MS.gene63309:CDS 65.0%
GAAGGAGGAGGACGAGGAGG+TGG + chr3.3:60280614-60280633 None:intergenic 65.0%
GATCCTGGTGGTCTCCCACG+TGG + chr3.3:60279948-60279967 None:intergenic 65.0%
GGTCAGGGGCAGGTTGTTGG+TGG - chr3.3:60280278-60280297 MS.gene63309:CDS 65.0%
GGTGGTCAGGGGCAGGTTGT+TGG - chr3.3:60280275-60280294 MS.gene63309:CDS 65.0%
GTAGCTCCAGCCGGAGAAGG+CGG + chr3.3:60280233-60280252 None:intergenic 65.0%
AGACAGCCGGCTGCTCCTGG+TGG - chr3.3:60280149-60280168 MS.gene63309:CDS 70.0%
AGGCCACGTGGGAGACCACC+AGG - chr3.3:60279942-60279961 MS.gene63309:CDS 70.0%
AGTGGTGGTCGGAGGCCACG+TGG - chr3.3:60279930-60279949 MS.gene63309:CDS 70.0%
CCGCCGTCACCGTGGTGTGT+CGG - chr3.3:60280088-60280107 MS.gene63309:CDS 70.0%
CTACCTCCGCCTTCTCCGGC+TGG - chr3.3:60280224-60280243 MS.gene63309:CDS 70.0%
GGAGGAGGACGAGGAGGTGG+AGG + chr3.3:60280611-60280630 None:intergenic 70.0%
GTGGTGGTCGGAGGCCACGT+GGG - chr3.3:60279931-60279950 MS.gene63309:CDS 70.0%
! CCGACACACCACGGTGACGG+CGG + chr3.3:60280091-60280110 None:intergenic 70.0%
GCTCCAGCCGGAGAAGGCGG+AGG + chr3.3:60280230-60280249 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr3.3 gene 60279714 60280640 60279714 ID=MS.gene63309
chr3.3 mRNA 60279714 60280640 60279714 ID=MS.gene63309.t1;Parent=MS.gene63309
chr3.3 exon 60279714 60280640 60279714 ID=MS.gene63309.t1.exon1;Parent=MS.gene63309.t1
chr3.3 CDS 60279714 60280640 60279714 ID=cds.MS.gene63309.t1;Parent=MS.gene63309.t1
Gene Sequence

>MS.gene63309

ATGGCAAATCGTTGGTGGGCTGGAAATGTGGCTATGGGTCACAGAGAATCAATGCCTTCATCACCATCATCACTTGAACATCTGAAAAGAGAGCAAGAGTTGATGGAAAACAACAACGCGAACTCAACTCCAACAAACAGCAGCAACAGCAACAACAACAACAACGAAGAGGAAGACAACAACAACGAAGAACAAAACCCATCTGAGCCAGGTAGCAGTGGTGGTCGGAGGCCACGTGGGAGACCACCAGGATCCAAAAACAAACCCAAACCACCCATTGTTATAACCAAAGAATCCCCCAACGCGCTAAGAAGCCATGTCTTGGAAATCAGCAGTGGTAGTGATGTGGCGGAGAGTATTGCCAACTTTGCTAACCGCCGTCACCGTGGTGTGTCGGTTTTGAGTGGGAGCGGCATTGTAGCTAATGTAACCCTTAGACAGCCGGCTGCTCCTGGTGGTGTTATTACTCTTCATGGAAGATTCGAGATACTTTCGCTTTCGGGTGCTTTTCTACCTCCGCCTTCTCCGGCTGGAGCTACTGGATTAACTGTTTATTTAGCTGGTGGTCAGGGGCAGGTTGTTGGTGGTTCTGTTGCAGGTCCTTTGGTTGCTTCTGGACCTGTTATGGTTATTGCTGCTACTTTTGCTAATGCTACTTATGAGAGGTTGCCCTTGGAAGATGAACAAGGTGGTGGTGATCAAGAGGAAATGCAAGTTCAACAATCTGGTGTGGATTCAGCAACTGTTGCAGCTGCAGTTACTAGTTCTTCTTCTCAAGGTTTGGAATTGGGTGAGCATCATCAACAACTTCAACAGGTTTCAATGCCTATGTATAATTTGCCTCCAAATTTGTTGCATAATGGTACTCAAATGCCTCATGATGTGTTTTGGGGTCCTCCACCTCCTCGTCCTCCTCCTTCTTTCTGA
Protein sequence

>MS.gene63309.t1

MANRWWAGNVAMGHRESMPSSPSSLEHLKREQELMENNNANSTPTNSSNSNNNNNEEEDNNNEEQNPSEPGSSGGRRPRGRPPGSKNKPKPPIVITKESPNALRSHVLEISSGSDVAESIANFANRRHRGVSVLSGSGIVANVTLRQPAAPGGVITLHGRFEILSLSGAFLPPPSPAGATGLTVYLAGGQGQVVGGSVAGPLVASGPVMVIAATFANATYERLPLEDEQGGGDQEEMQVQQSGVDSATVAAAVTSSSSQGLELGEHHQQLQQVSMPMYNLPPNLLHNGTQMPHDVFWGPPPPRPPPSF