Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63438.t1 | XP_013462517.1 | 81.1 | 74 | 14 | 0 | 16 | 89 | 262 | 335 | 1.00E-22 | 115.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63438.t1 | A0A072VEE6 | 81.1 | 74 | 14 | 0 | 16 | 89 | 283 | 356 | 7.5e-23 | 115.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051744 | MS.gene63438 | 0.81105 | 8.50E-51 | -1.69E-46 |
| MS.gene052020 | MS.gene63438 | 0.806097 | 9.70E-50 | -1.69E-46 |
| MS.gene052519 | MS.gene63438 | 0.80981 | 1.57E-50 | -1.69E-46 |
| MS.gene052863 | MS.gene63438 | 0.806629 | 7.50E-50 | -1.69E-46 |
| MS.gene053900 | MS.gene63438 | 0.800411 | 1.46E-48 | -1.69E-46 |
| MS.gene054236 | MS.gene63438 | 0.801885 | 7.29E-49 | -1.69E-46 |
| MS.gene054651 | MS.gene63438 | 0.801453 | 8.94E-49 | -1.69E-46 |
| MS.gene055818 | MS.gene63438 | 0.830085 | 3.58E-55 | -1.69E-46 |
| MS.gene056050 | MS.gene63438 | 0.809314 | 2.01E-50 | -1.69E-46 |
| MS.gene056366 | MS.gene63438 | 0.811466 | 6.90E-51 | -1.69E-46 |
| MS.gene058625 | MS.gene63438 | 0.801242 | 9.88E-49 | -1.69E-46 |
| MS.gene058979 | MS.gene63438 | 0.808977 | 2.38E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63438.t1 | MTR_2g013980 | 81.081 | 74 | 14 | 0 | 16 | 89 | 287 | 360 | 2.15e-32 | 118 |
| MS.gene63438.t1 | MTR_2g013980 | 81.081 | 74 | 14 | 0 | 16 | 89 | 262 | 335 | 2.19e-32 | 118 |
| MS.gene63438.t1 | MTR_2g013980 | 81.081 | 74 | 14 | 0 | 16 | 89 | 262 | 335 | 2.27e-32 | 118 |
| MS.gene63438.t1 | MTR_2g013980 | 81.081 | 74 | 14 | 0 | 16 | 89 | 283 | 356 | 2.32e-32 | 118 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 18 sgRNAs with CRISPR-Local
Find 78 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCCAAAGCTATGGTCTCTTA+TGG | 0.377011 | 6.3:+78956357 | MS.gene63438:CDS |
| TACCGTCAAATCGCAACCTT+TGG | 0.397124 | 6.3:-78955563 | None:intergenic |
| ACCATAAGAGACCATAGCTT+TGG | 0.399739 | 6.3:-78956358 | None:intergenic |
| AGCCAAAGGTTGCGATTTGA+CGG | 0.449820 | 6.3:+78955561 | MS.gene63438:CDS |
| GCAGGCAAGAAGGTTTCTAC+AGG | 0.468697 | 6.3:+78956958 | MS.gene63438:intron |
| CCATAAGAGACCATAGCTTT+GGG | 0.474083 | 6.3:-78956357 | None:intergenic |
| AAGAATCAGTGAAGAGAGGA+AGG | 0.520264 | 6.3:+78956270 | MS.gene63438:CDS |
| GTTTCCTCTTCATAACATCA+AGG | 0.539667 | 6.3:+78956392 | MS.gene63438:CDS |
| CTACAGGGTTATATTTGTGA+GGG | 0.565052 | 6.3:+78956974 | MS.gene63438:CDS |
| ATGGTATACTGCAGGCAAGA+AGG | 0.565571 | 6.3:+78956948 | MS.gene63438:intron |
| TCACTTGAAACATATTTCAG+AGG | 0.566829 | 6.3:-78956217 | None:intergenic |
| TCTACAGGGTTATATTTGTG+AGG | 0.566932 | 6.3:+78956973 | MS.gene63438:CDS |
| ATTTCACTGACCCAAAGCTA+TGG | 0.570148 | 6.3:+78956347 | MS.gene63438:CDS |
| ACGGCTCATGGCGGAAAATG+TGG | 0.592325 | 6.3:+78955531 | None:intergenic |
| TATACCTTGATGTTATGAAG+AGG | 0.596127 | 6.3:-78956396 | None:intergenic |
| AATGTGGCTCTTACAGCCAA+AGG | 0.613003 | 6.3:+78955547 | MS.gene63438:CDS |
| CTACAAGAATCAGTGAAGAG+AGG | 0.631009 | 6.3:+78956266 | MS.gene63438:CDS |
| CAGGCAAGAAGGTTTCTACA+GGG | 0.650713 | 6.3:+78956959 | MS.gene63438:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATAATAAATATTAGAATAGT+AGG | + | chr6.3:78955930-78955949 | MS.gene63438:intron | 10.0% |
| !! | AATAAATTGTCGTAAAATTA+AGG | - | chr6.3:78956611-78956630 | None:intergenic | 15.0% |
| !!! | ATAATTTTTTTACTCATAAG+TGG | + | chr6.3:78956085-78956104 | MS.gene63438:intron | 15.0% |
| !! | AATTGTTGTAATTAAAACCT+TGG | - | chr6.3:78956645-78956664 | None:intergenic | 20.0% |
| !! | ATGAGTAAAAAAATTATCTG+AGG | - | chr6.3:78956082-78956101 | None:intergenic | 20.0% |
| !! | TAGAATGATAAATTAGTAAG+AGG | - | chr6.3:78955893-78955912 | None:intergenic | 20.0% |
| !! | TCTTACTAATTTATCATTCT+AGG | + | chr6.3:78955892-78955911 | MS.gene63438:intron | 20.0% |
| !! | TGAGTAAAAAAATTATCTGA+GGG | - | chr6.3:78956081-78956100 | None:intergenic | 20.0% |
| !! | TTTACGACAATTTATTAGAT+AGG | + | chr6.3:78956615-78956634 | MS.gene63438:intron | 20.0% |
| !!! | GTTAAGTTTTTTATTGCTAT+GGG | + | chr6.3:78955652-78955671 | MS.gene63438:intron | 20.0% |
| ! | AGTATACCATAATAGCTTAA+AGG | - | chr6.3:78956938-78956957 | None:intergenic | 25.0% |
| ! | ATAAAAAACTTAACCATCAC+AGG | - | chr6.3:78955646-78955665 | None:intergenic | 25.0% |
| ! | GGAAATTCAAACAGTAAAAA+AGG | - | chr6.3:78956476-78956495 | None:intergenic | 25.0% |
| ! | GTATACCATAATAGCTTAAA+GGG | - | chr6.3:78956937-78956956 | None:intergenic | 25.0% |
| ! | TTTCATAATCACACAATCTA+GGG | - | chr6.3:78955698-78955717 | None:intergenic | 25.0% |
| !! | ACTTTTAAGCATACCTATTA+TGG | + | chr6.3:78956876-78956895 | MS.gene63438:intron | 25.0% |
| !! | GGTTAAATTTGAAATGAGAA+TGG | + | chr6.3:78955718-78955737 | MS.gene63438:intron | 25.0% |
| !! | TTTTTACTCATAAGTGGAAA+TGG | + | chr6.3:78956091-78956110 | MS.gene63438:intron | 25.0% |
| !!! | GGTTAAGTTTTTTATTGCTA+TGG | + | chr6.3:78955651-78955670 | MS.gene63438:intron | 25.0% |
| !!! | GTTTTGTTCTAGAATTTTCT+TGG | + | chr6.3:78956438-78956457 | MS.gene63438:intron | 25.0% |
| !!! | TGATATCTGAATTAGTTTTG+AGG | + | chr6.3:78956742-78956761 | MS.gene63438:intron | 25.0% |
| AAGATCAAAAACAGCACTTA+AGG | - | chr6.3:78956181-78956200 | None:intergenic | 30.0% | |
| AAGCTCAAAAGAGTTAGAAT+GGG | + | chr6.3:78956025-78956044 | MS.gene63438:intron | 30.0% | |
| AAGGACATGATGATATCTAT+TGG | + | chr6.3:78955786-78955805 | MS.gene63438:intron | 30.0% | |
| AATATGAAGAGCAGGAAATA+AGG | - | chr6.3:78955614-78955633 | None:intergenic | 30.0% | |
| AATGTCCCTTTAAGCTATTA+TGG | + | chr6.3:78956929-78956948 | MS.gene63438:intron | 30.0% | |
| AGATCAGAAATATCAAACAC+AGG | - | chr6.3:78956142-78956161 | None:intergenic | 30.0% | |
| AGTAGGACTCTCATAATATT+AGG | + | chr6.3:78955947-78955966 | MS.gene63438:intron | 30.0% | |
| ATAGAACTAAAGACCATGTT+GGG | + | chr6.3:78956827-78956846 | MS.gene63438:intron | 30.0% | |
| CAAGTCTCATAAACCATAAT+AGG | - | chr6.3:78956892-78956911 | None:intergenic | 30.0% | |
| CATAACATCAAGGTATATCT+TGG | + | chr6.3:78956402-78956421 | MS.gene63438:intron | 30.0% | |
| CTAGATTGTGTGATTATGAA+AGG | + | chr6.3:78955697-78955716 | MS.gene63438:intron | 30.0% | |
| CTCTCATAATATTAGGACAT+AGG | + | chr6.3:78955954-78955973 | MS.gene63438:intron | 30.0% | |
| CTTTCATAATCACACAATCT+AGG | - | chr6.3:78955699-78955718 | None:intergenic | 30.0% | |
| TATACCTTGATGTTATGAAG+AGG | - | chr6.3:78956399-78956418 | None:intergenic | 30.0% | |
| TATAGAACTAAAGACCATGT+TGG | + | chr6.3:78956826-78956845 | MS.gene63438:intron | 30.0% | |
| TCACTTGAAACATATTTCAG+AGG | - | chr6.3:78956220-78956239 | None:intergenic | 30.0% | |
| TGAGGAATTCTTTATTGCAT+TGG | + | chr6.3:78956760-78956779 | MS.gene63438:intron | 30.0% | |
| TTTATGAGACTTGATTTCTG+TGG | + | chr6.3:78956899-78956918 | MS.gene63438:intron | 30.0% | |
| TTTATTAGATAGGAATGCCA+AGG | + | chr6.3:78956625-78956644 | MS.gene63438:intron | 30.0% | |
| ! | ATCATTCTAGGAAGTAATTG+AGG | + | chr6.3:78955904-78955923 | MS.gene63438:intron | 30.0% |
| !! | TGTTTTTGATCTTGCAGAAT+AGG | + | chr6.3:78956188-78956207 | MS.gene63438:intron | 30.0% |
| AACGATGTAGCTACTAGAAA+TGG | + | chr6.3:78955858-78955877 | MS.gene63438:intron | 35.0% | |
| AAGCTATTATGGTATACTGC+AGG | + | chr6.3:78956940-78956959 | MS.gene63438:intron | 35.0% | |
| ACGATGTAGCTACTAGAAAT+GGG | + | chr6.3:78955859-78955878 | MS.gene63438:intron | 35.0% | |
| AGAAGGATTGAAGCAAATCA+AGG | - | chr6.3:78955757-78955776 | None:intergenic | 35.0% | |
| AGACTTGATTTCTGTGGATT+TGG | + | chr6.3:78956905-78956924 | MS.gene63438:intron | 35.0% | |
| ATTCATTTGCAGTCTGTTCA+AGG | + | chr6.3:78955828-78955847 | MS.gene63438:intron | 35.0% | |
| CAAAAGAGTTAGAATGGGAT+AGG | + | chr6.3:78956030-78956049 | MS.gene63438:intron | 35.0% | |
| CACTAATCAATATGAAGAGC+AGG | - | chr6.3:78955622-78955641 | None:intergenic | 35.0% | |
| CTACAGGGTTATATTTGTGA+GGG | + | chr6.3:78956974-78956993 | MS.gene63438:CDS | 35.0% | |
| GAAGCTCAAAAGAGTTAGAA+TGG | + | chr6.3:78956024-78956043 | MS.gene63438:intron | 35.0% | |
| GTTTCCTCTTCATAACATCA+AGG | + | chr6.3:78956392-78956411 | MS.gene63438:CDS | 35.0% | |
| TCTACAGGGTTATATTTGTG+AGG | + | chr6.3:78956973-78956992 | MS.gene63438:CDS | 35.0% | |
| TTCATTTGCAGTCTGTTCAA+GGG | + | chr6.3:78955829-78955848 | MS.gene63438:intron | 35.0% | |
| ! | TATTGATTAGTGACCTGTGA+TGG | + | chr6.3:78955630-78955649 | MS.gene63438:intron | 35.0% |
| ! | TGACTTAATTTGACTGCAAG+AGG | - | chr6.3:78956497-78956516 | None:intergenic | 35.0% |
| ! | TTGAAGCAAATCAAGGTATC+AGG | - | chr6.3:78955750-78955769 | None:intergenic | 35.0% |
| AACTGATTGCGAACGTAGAT+AGG | - | chr6.3:78956855-78956874 | None:intergenic | 40.0% | |
| AAGAATCAGTGAAGAGAGGA+AGG | + | chr6.3:78956270-78956289 | MS.gene63438:CDS | 40.0% | |
| ACCATAAGAGACCATAGCTT+TGG | - | chr6.3:78956361-78956380 | None:intergenic | 40.0% | |
| ATGTCCTTAAGATCGACAGA+AGG | - | chr6.3:78955774-78955793 | None:intergenic | 40.0% | |
| ATTTCACTGACCCAAAGCTA+TGG | + | chr6.3:78956347-78956366 | MS.gene63438:CDS | 40.0% | |
| CAATCCTTCTGTCGATCTTA+AGG | + | chr6.3:78955767-78955786 | MS.gene63438:intron | 40.0% | |
| CCATAAGAGACCATAGCTTT+GGG | - | chr6.3:78956360-78956379 | None:intergenic | 40.0% | |
| CGATGTAGCTACTAGAAATG+GGG | + | chr6.3:78955860-78955879 | MS.gene63438:intron | 40.0% | |
| CTACAAGAATCAGTGAAGAG+AGG | + | chr6.3:78956266-78956285 | MS.gene63438:CDS | 40.0% | |
| GGCTTCTACAATTGTCAAGT+TGG | + | chr6.3:78956781-78956800 | MS.gene63438:intron | 40.0% | |
| ! | ACTCTTTTGAGCTTCAGTCT+TGG | - | chr6.3:78956019-78956038 | None:intergenic | 40.0% |
| AATGTGGCTCTTACAGCCAA+AGG | + | chr6.3:78955547-78955566 | MS.gene63438:CDS | 45.0% | |
| AGCCAAAGGTTGCGATTTGA+CGG | + | chr6.3:78955561-78955580 | MS.gene63438:CDS | 45.0% | |
| AGTTAGAATGGGATAGGCAC+AGG | + | chr6.3:78956036-78956055 | MS.gene63438:intron | 45.0% | |
| ATGGTATACTGCAGGCAAGA+AGG | + | chr6.3:78956948-78956967 | MS.gene63438:intron | 45.0% | |
| CAGGCAAGAAGGTTTCTACA+GGG | + | chr6.3:78956959-78956978 | MS.gene63438:intron | 45.0% | |
| CCCAAAGCTATGGTCTCTTA+TGG | + | chr6.3:78956357-78956376 | MS.gene63438:CDS | 45.0% | |
| TACCGTCAAATCGCAACCTT+TGG | - | chr6.3:78955566-78955585 | None:intergenic | 45.0% | |
| ACGTAGATAGGAGCCCAACA+TGG | - | chr6.3:78956843-78956862 | None:intergenic | 50.0% | |
| GCAGGCAAGAAGGTTTCTAC+AGG | + | chr6.3:78956958-78956977 | MS.gene63438:intron | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.3 | gene | 78955538 | 78957007 | 78955538 | ID=MS.gene63438 |
| chr6.3 | mRNA | 78955538 | 78957007 | 78955538 | ID=MS.gene63438.t1;Parent=MS.gene63438 |
| chr6.3 | exon | 78955538 | 78955582 | 78955538 | ID=MS.gene63438.t1.exon1;Parent=MS.gene63438.t1 |
| chr6.3 | CDS | 78955538 | 78955582 | 78955538 | ID=cds.MS.gene63438.t1;Parent=MS.gene63438.t1 |
| chr6.3 | exon | 78956205 | 78956413 | 78956205 | ID=MS.gene63438.t1.exon2;Parent=MS.gene63438.t1 |
| chr6.3 | CDS | 78956205 | 78956413 | 78956205 | ID=cds.MS.gene63438.t1;Parent=MS.gene63438.t1 |
| chr6.3 | exon | 78956962 | 78957007 | 78956962 | ID=MS.gene63438.t1.exon3;Parent=MS.gene63438.t1 |
| chr6.3 | CDS | 78956962 | 78957007 | 78956962 | ID=cds.MS.gene63438.t1;Parent=MS.gene63438.t1 |
| Gene Sequence |
| Protein sequence |
