Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63809.t1 | AET00594.2 | 91.2 | 272 | 23 | 1 | 279 | 549 | 40 | 311 | 9.70E-102 | 380.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63809.t1 | Q9FLP3 | 64.4 | 180 | 64 | 0 | 365 | 544 | 114 | 293 | 3.2e-53 | 211.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63809.t1 | G7K8P1 | 91.2 | 272 | 23 | 1 | 279 | 549 | 40 | 311 | 7.0e-102 | 380.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049824 | MS.gene63809 | 0.811432 | 7.02E-51 | -1.69E-46 |
| MS.gene049825 | MS.gene63809 | 0.8263 | 2.93E-54 | -1.69E-46 |
| MS.gene049873 | MS.gene63809 | 0.818781 | 1.64E-52 | -1.69E-46 |
| MS.gene052584 | MS.gene63809 | 0.811638 | 6.33E-51 | -1.69E-46 |
| MS.gene053226 | MS.gene63809 | 0.815691 | 8.12E-52 | -1.69E-46 |
| MS.gene05365 | MS.gene63809 | 0.803964 | 2.71E-49 | -1.69E-46 |
| MS.gene055589 | MS.gene63809 | 0.800701 | 1.27E-48 | -1.69E-46 |
| MS.gene055778 | MS.gene63809 | 0.802537 | 5.35E-49 | -1.69E-46 |
| MS.gene058908 | MS.gene63809 | 0.80814 | 3.59E-50 | -1.69E-46 |
| MS.gene058911 | MS.gene63809 | 0.804628 | 1.97E-49 | -1.69E-46 |
| MS.gene05954 | MS.gene63809 | 0.812226 | 4.72E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 107 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTCACTTGATGTATCAATTT+TGG | 0.095462 | 5.3:-75770380 | None:intergenic |
| TTCAAGAGCTTCAACAAATT+TGG | 0.143820 | 5.3:+75761531 | MS.gene63809:CDS |
| ACTACTTCCCATTTATTAAA+AGG | 0.156322 | 5.3:-75764027 | None:intergenic |
| ACTTGATGTTGCGGATAATT+TGG | 0.165016 | 5.3:+75770324 | MS.gene63809:CDS |
| CGTTTCGACTTCAACATATT+TGG | 0.197420 | 5.3:+75764326 | MS.gene63809:CDS |
| AGAGTTAAATTTACCTGATT+TGG | 0.208283 | 5.3:-75761702 | None:intergenic |
| TTGGAAGAACTAGAGAAATT+TGG | 0.233939 | 5.3:+75761550 | MS.gene63809:CDS |
| CCCTCACGCTGGATTTGATA+TGG | 0.285390 | 5.3:+75761663 | MS.gene63809:CDS |
| AAGAACCAACATCAAACTTT+TGG | 0.299666 | 5.3:-75764155 | None:intergenic |
| TAAATGGGAAGTAGTGAAAT+TGG | 0.303632 | 5.3:+75764035 | MS.gene63809:CDS |
| AGCACAGCTGCAACAGTGCC+TGG | 0.313047 | 5.3:-75771601 | None:intergenic |
| AAAGAGGATTCGGATCTAAT+TGG | 0.315457 | 5.3:+75764130 | MS.gene63809:CDS |
| TTGGGTGCTTGTGGGCACTA+TGG | 0.322723 | 5.3:-75761600 | None:intergenic |
| AGCAGAGAGAAGCGAGGTTC+GGG | 0.323559 | 5.3:-75766210 | None:intergenic |
| CTTGATGTTGCGGATAATTT+GGG | 0.353085 | 5.3:+75770325 | MS.gene63809:CDS |
| TGCTTCATGATCGTGTTATT+CGG | 0.355131 | 5.3:+75772183 | MS.gene63809:CDS |
| GAAACTCCTGAAGACACTTC+TGG | 0.368193 | 5.3:+75770417 | MS.gene63809:CDS |
| CTATCTTTAGCAAATGCATT+TGG | 0.372236 | 5.3:+75761335 | None:intergenic |
| TTCAAGGAAATTCGCTATAC+AGG | 0.393442 | 5.3:+75770211 | MS.gene63809:CDS |
| GGATCAAAGGGTTCATTTGT+AGG | 0.395749 | 5.3:-75771532 | None:intergenic |
| CACCTTCCAGAAGTGTCTTC+AGG | 0.396210 | 5.3:-75770423 | None:intergenic |
| GAGCAGAGAGAAGCGAGGTT+CGG | 0.396337 | 5.3:-75766211 | None:intergenic |
| TCTCCGTACTTACGCTGAAA+TGG | 0.407067 | 5.3:+75770151 | MS.gene63809:CDS |
| TCTTCAGACTTGGCTTGTCC+AGG | 0.407793 | 5.3:-75769918 | None:intergenic |
| AGAATAACTTGAACCAAATC+AGG | 0.409983 | 5.3:+75761689 | MS.gene63809:CDS |
| AGAGAAAGCTGGTTTGATAT+TGG | 0.414884 | 5.3:+75764059 | MS.gene63809:CDS |
| AATGTAGAAATGAACCTTGT+TGG | 0.417880 | 5.3:-75766303 | None:intergenic |
| GAGAAGCGAGGTTCGGGAAA+TGG | 0.422213 | 5.3:-75766204 | None:intergenic |
| CATCACCCGAAGTTGGTGTT+GGG | 0.427313 | 5.3:+75763934 | MS.gene63809:CDS |
| GAAGATCAAACAGAAGAATC+AGG | 0.432003 | 5.3:+75769936 | MS.gene63809:CDS |
| TGGGTGCTTGTGGGCACTAT+GGG | 0.434155 | 5.3:-75761599 | None:intergenic |
| GACGTGAAGCAGAGAATTCA+AGG | 0.434720 | 5.3:+75770195 | MS.gene63809:CDS |
| GATGAGGGAATGTAATTGAT+TGG | 0.436632 | 5.3:-75766267 | None:intergenic |
| TTCTGTTTGATCTTCAGACT+TGG | 0.446854 | 5.3:-75769928 | None:intergenic |
| ACAGTGAATCGGATCTATCC+AGG | 0.450640 | 5.3:+75770045 | MS.gene63809:CDS |
| CTACTTCCCATTTATTAAAA+GGG | 0.451717 | 5.3:-75764026 | None:intergenic |
| TACGCAAATAAAAGAGGATT+CGG | 0.452936 | 5.3:+75764120 | MS.gene63809:CDS |
| ATCATTGAATGAAGATCATA+TGG | 0.457962 | 5.3:+75764299 | MS.gene63809:CDS |
| GAAGGGAATAATGTGCATAA+TGG | 0.461273 | 5.3:+75769837 | MS.gene63809:CDS |
| CACATTCTCAAACTGTCAAA+AGG | 0.467135 | 5.3:+75769976 | MS.gene63809:CDS |
| GCACAGCTGCAACAGTGCCT+GGG | 0.468538 | 5.3:-75771600 | None:intergenic |
| GTGAAATTGGCAGAGAAAGC+TGG | 0.471686 | 5.3:+75764048 | MS.gene63809:CDS |
| AGAAAACCCAACACCAACTT+CGG | 0.472302 | 5.3:-75763940 | None:intergenic |
| AACACATTCAAATTCTCAAA+AGG | 0.473855 | 5.3:+75764180 | MS.gene63809:CDS |
| GCATCACCCGAAGTTGGTGT+TGG | 0.474874 | 5.3:+75763933 | MS.gene63809:CDS |
| TTGTAACATTGGGTGCTTGT+GGG | 0.483636 | 5.3:-75761608 | None:intergenic |
| AAAAGAGATTGAGAAAATGC+AGG | 0.485423 | 5.3:+75770121 | MS.gene63809:CDS |
| ATAACAATCCAGCTGAATGA+TGG | 0.497132 | 5.3:+75772240 | MS.gene63809:CDS |
| ACAAAGAATGAACCGAGTCT+TGG | 0.498768 | 5.3:-75764362 | None:intergenic |
| TGGAACCAAAAGTTTGATGT+TGG | 0.504024 | 5.3:+75764150 | MS.gene63809:CDS |
| GTTATTCGGCCAGCGGAAGT+TGG | 0.504351 | 5.3:+75772197 | MS.gene63809:CDS |
| GAAAACCCAACACCAACTTC+GGG | 0.508541 | 5.3:-75763939 | None:intergenic |
| CACAGCTGCAACAGTGCCTG+GGG | 0.509586 | 5.3:-75771599 | None:intergenic |
| TCTCCATTTCAGCGTAAGTA+CGG | 0.510972 | 5.3:-75770154 | None:intergenic |
| ACGAGAAGTTGCTACTATGT+TGG | 0.513026 | 5.3:-75761366 | None:intergenic |
| GATCAAAGGGTTCATTTGTA+GGG | 0.515825 | 5.3:-75771531 | None:intergenic |
| TGGTTTGATATTGGTTGAGA+AGG | 0.517079 | 5.3:+75764068 | MS.gene63809:CDS |
| GTTGAAACAGAAGGGAAAGA+AGG | 0.519141 | 5.3:+75769819 | MS.gene63809:CDS |
| GATTAAGCTTGTAGCTGAGA+AGG | 0.524422 | 5.3:+75770076 | MS.gene63809:CDS |
| CCATGTTACGCAAATAAAAG+AGG | 0.525629 | 5.3:+75764114 | MS.gene63809:CDS |
| AAGCTTAATCAAATCATCCC+TGG | 0.527767 | 5.3:-75770063 | None:intergenic |
| TGGGTTACACCAACTTCCGC+TGG | 0.528802 | 5.3:-75772206 | None:intergenic |
| GTCATGCCTATGTGGATCAA+AGG | 0.531278 | 5.3:-75771545 | None:intergenic |
| TTGAAACAGAAGGGAAAGAA+GGG | 0.534188 | 5.3:+75769820 | MS.gene63809:CDS |
| GCAGAGGAAATTGATCAGCC+TGG | 0.537322 | 5.3:+75769900 | MS.gene63809:CDS |
| TTTGTAACATTGGGTGCTTG+TGG | 0.537585 | 5.3:-75761609 | None:intergenic |
| AGGAAGATGTTAAGTTGTAA+TGG | 0.539279 | 5.3:+75763973 | MS.gene63809:CDS |
| GTCTATAATTTCCCTCACGC+TGG | 0.541608 | 5.3:+75761652 | MS.gene63809:CDS |
| AAATGGAGAATGTCATGGAC+AGG | 0.541642 | 5.3:+75770168 | MS.gene63809:CDS |
| CTCAAAAGGAAGAGATATTG+TGG | 0.543556 | 5.3:+75764194 | MS.gene63809:CDS |
| AAAGAGTTTACTTGATGTTG+CGG | 0.544338 | 5.3:+75770315 | MS.gene63809:CDS |
| AGCTGTGGAAGTGTTGTTGA+GGG | 0.545466 | 5.3:-75766241 | None:intergenic |
| TTCAGATTCAGACAGTGAAT+CGG | 0.546821 | 5.3:+75770034 | MS.gene63809:CDS |
| TCATGCCTATGTGGATCAAA+GGG | 0.547596 | 5.3:-75771544 | None:intergenic |
| TTTCCCTTCTGTTTCAACTG+AGG | 0.548286 | 5.3:-75769814 | None:intergenic |
| AAGTGTTGTTGAGGGATTCG+CGG | 0.548610 | 5.3:-75766233 | None:intergenic |
| CGCTGAAATGGAGAATGTCA+TGG | 0.553186 | 5.3:+75770163 | MS.gene63809:CDS |
| TGAACCTTGTTGGTAGAATG+AGG | 0.554586 | 5.3:-75766293 | None:intergenic |
| ATCAAGTGACTCCGCTGAAG+CGG | 0.555608 | 5.3:+75770393 | MS.gene63809:CDS |
| CATTCCTCATTCTACCAACA+AGG | 0.561352 | 5.3:+75766289 | MS.gene63809:CDS |
| CTCAAACTGTCAAAAGGAGG+AGG | 0.568785 | 5.3:+75769982 | MS.gene63809:CDS |
| AAACTGTCAAAAGGAGGAGG+AGG | 0.571090 | 5.3:+75769985 | MS.gene63809:CDS |
| CACTGTTGCAGCTGTGCTGA+AGG | 0.572042 | 5.3:+75771606 | MS.gene63809:CDS |
| TCAGCCTCAGTTGAAACAGA+AGG | 0.575095 | 5.3:+75769810 | MS.gene63809:intron |
| GTGCAGGCATCACCCGAAGT+TGG | 0.577764 | 5.3:+75763927 | MS.gene63809:intron |
| CAGGAGTTTCACCGCTTCAG+CGG | 0.583821 | 5.3:-75770404 | None:intergenic |
| AAGCTGTGGAAGTGTTGTTG+AGG | 0.585156 | 5.3:-75766242 | None:intergenic |
| TCAAAATACGATCCAAGACT+CGG | 0.591630 | 5.3:+75764350 | MS.gene63809:CDS |
| TAGTCGACCCAAACTCACTT+CGG | 0.594497 | 5.3:+75764244 | MS.gene63809:CDS |
| GCAGTCTTCGAAGTTCCTGA+TGG | 0.602698 | 5.3:+75771568 | MS.gene63809:CDS |
| GGTAGAATGAGGAATGATGA+GGG | 0.603929 | 5.3:-75766282 | None:intergenic |
| CAGCCTCAGTTGAAACAGAA+GGG | 0.605878 | 5.3:+75769811 | MS.gene63809:intron |
| GACTGAGAAACAACTTTCAG+AGG | 0.609291 | 5.3:+75770453 | MS.gene63809:CDS |
| AAGACTGCGTCATGCCTATG+TGG | 0.609345 | 5.3:-75771553 | None:intergenic |
| GTAATTGATTGGAGAAGCTG+TGG | 0.610688 | 5.3:-75766256 | None:intergenic |
| GCCATATCAAATCCAGCGTG+AGG | 0.617235 | 5.3:-75761664 | None:intergenic |
| CCATATCAAATCCAGCGTGA+GGG | 0.626856 | 5.3:-75761663 | None:intergenic |
| TGATCGTGTTATTCGGCCAG+CGG | 0.642674 | 5.3:+75772190 | MS.gene63809:CDS |
| CTCCTGAAGACACTTCTGGA+AGG | 0.644196 | 5.3:+75770421 | MS.gene63809:CDS |
| CAGTGAATCGGATCTATCCA+GGG | 0.646702 | 5.3:+75770046 | MS.gene63809:CDS |
| TGGTAGAATGAGGAATGATG+AGG | 0.653665 | 5.3:-75766283 | None:intergenic |
| GCAACTTCTCGTGATTCCAA+AGG | 0.663324 | 5.3:+75761377 | MS.gene63809:CDS |
| ATTCTCAAACTGTCAAAAGG+AGG | 0.665800 | 5.3:+75769979 | MS.gene63809:CDS |
| TCGCGGAGCAGAGAGAAGCG+AGG | 0.670556 | 5.3:-75766216 | None:intergenic |
| ATGAACCCTTTGATCCACAT+AGG | 0.670564 | 5.3:+75771539 | MS.gene63809:CDS |
| ACAGCTGCAACAGTGCCTGG+GGG | 0.671502 | 5.3:-75771598 | None:intergenic |
| TCACGCTGGATTTGATATGG+CGG | 0.689213 | 5.3:+75761666 | MS.gene63809:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 75761345 | 75772259 | 75761345 | ID=MS.gene63809 |
| chr5.3 | mRNA | 75761345 | 75772259 | 75761345 | ID=MS.gene63809.t1;Parent=MS.gene63809 |
| chr5.3 | exon | 75761345 | 75761398 | 75761345 | ID=MS.gene63809.t1.exon1;Parent=MS.gene63809.t1 |
| chr5.3 | CDS | 75761345 | 75761398 | 75761345 | ID=cds.MS.gene63809.t1;Parent=MS.gene63809.t1 |
| chr5.3 | exon | 75761512 | 75761710 | 75761512 | ID=MS.gene63809.t1.exon2;Parent=MS.gene63809.t1 |
| chr5.3 | CDS | 75761512 | 75761710 | 75761512 | ID=cds.MS.gene63809.t1;Parent=MS.gene63809.t1 |
| chr5.3 | exon | 75763933 | 75764418 | 75763933 | ID=MS.gene63809.t1.exon3;Parent=MS.gene63809.t1 |
| chr5.3 | CDS | 75763933 | 75764418 | 75763933 | ID=cds.MS.gene63809.t1;Parent=MS.gene63809.t1 |
| chr5.3 | exon | 75766201 | 75766310 | 75766201 | ID=MS.gene63809.t1.exon4;Parent=MS.gene63809.t1 |
| chr5.3 | CDS | 75766201 | 75766310 | 75766201 | ID=cds.MS.gene63809.t1;Parent=MS.gene63809.t1 |
| chr5.3 | exon | 75769814 | 75770232 | 75769814 | ID=MS.gene63809.t1.exon5;Parent=MS.gene63809.t1 |
| chr5.3 | CDS | 75769814 | 75770232 | 75769814 | ID=cds.MS.gene63809.t1;Parent=MS.gene63809.t1 |
| chr5.3 | exon | 75770307 | 75770474 | 75770307 | ID=MS.gene63809.t1.exon6;Parent=MS.gene63809.t1 |
| chr5.3 | CDS | 75770307 | 75770474 | 75770307 | ID=cds.MS.gene63809.t1;Parent=MS.gene63809.t1 |
| chr5.3 | exon | 75771499 | 75771627 | 75771499 | ID=MS.gene63809.t1.exon7;Parent=MS.gene63809.t1 |
| chr5.3 | CDS | 75771499 | 75771627 | 75771499 | ID=cds.MS.gene63809.t1;Parent=MS.gene63809.t1 |
| chr5.3 | exon | 75772173 | 75772259 | 75772173 | ID=MS.gene63809.t1.exon8;Parent=MS.gene63809.t1 |
| chr5.3 | CDS | 75772173 | 75772259 | 75772173 | ID=cds.MS.gene63809.t1;Parent=MS.gene63809.t1 |
| Gene Sequence |
| Protein sequence |
