Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058908.t1 | XP_013446712.1 | 98.1 | 104 | 2 | 0 | 1 | 104 | 40 | 143 | 2.90E-47 | 197.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058908.t1 | A0A072TT93 | 98.1 | 104 | 2 | 0 | 1 | 104 | 40 | 143 | 2.1e-47 | 197.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049873 | MS.gene058908 | 0.803032 | 4.23E-49 | -1.69E-46 |
| MS.gene052376 | MS.gene058908 | 0.804502 | 2.09E-49 | -1.69E-46 |
| MS.gene055808 | MS.gene058908 | 0.82065 | 6.13E-53 | -1.69E-46 |
| MS.gene056091 | MS.gene058908 | 0.820754 | 5.80E-53 | -1.69E-46 |
| MS.gene056403 | MS.gene058908 | 0.808453 | 3.07E-50 | -1.69E-46 |
| MS.gene056605 | MS.gene058908 | 0.809655 | 1.70E-50 | -1.69E-46 |
| MS.gene058037 | MS.gene058908 | 0.806999 | 6.26E-50 | -1.69E-46 |
| MS.gene058908 | MS.gene058911 | 0.98149 | 1.02E-152 | -1.69E-46 |
| MS.gene058908 | MS.gene05954 | 0.803304 | 3.72E-49 | -1.69E-46 |
| MS.gene058908 | MS.gene059935 | 0.801675 | 8.05E-49 | -1.69E-46 |
| MS.gene058908 | MS.gene060429 | 0.812146 | 4.91E-51 | -1.69E-46 |
| MS.gene058908 | MS.gene062672 | 0.812265 | 4.63E-51 | -1.69E-46 |
| MS.gene058908 | MS.gene064038 | 0.809789 | 1.59E-50 | -1.69E-46 |
| MS.gene058908 | MS.gene066194 | 0.801938 | 7.11E-49 | -1.69E-46 |
| MS.gene058908 | MS.gene071987 | 0.834518 | 2.86E-56 | -1.69E-46 |
| MS.gene058908 | MS.gene072280 | 0.807023 | 6.19E-50 | -1.69E-46 |
| MS.gene058908 | MS.gene07719 | 0.811615 | 6.41E-51 | -1.69E-46 |
| MS.gene058908 | MS.gene09952 | 0.814685 | 1.36E-51 | -1.69E-46 |
| MS.gene058908 | MS.gene21149 | 0.80148 | 8.83E-49 | -1.69E-46 |
| MS.gene058908 | MS.gene21700 | 0.826694 | 2.36E-54 | -1.69E-46 |
| MS.gene058908 | MS.gene21926 | 0.829415 | 5.21E-55 | -1.69E-46 |
| MS.gene058908 | MS.gene21928 | 0.828951 | 6.76E-55 | -1.69E-46 |
| MS.gene058908 | MS.gene22270 | 0.83958 | 1.46E-57 | -1.69E-46 |
| MS.gene058908 | MS.gene24260 | 0.813103 | 3.03E-51 | -1.69E-46 |
| MS.gene058908 | MS.gene24492 | 0.804767 | 1.84E-49 | -1.69E-46 |
| MS.gene058908 | MS.gene24692 | 0.809697 | 1.66E-50 | -1.69E-46 |
| MS.gene058908 | MS.gene26979 | 0.805252 | 1.46E-49 | -1.69E-46 |
| MS.gene058908 | MS.gene27041 | 0.806278 | 8.89E-50 | -1.69E-46 |
| MS.gene058908 | MS.gene28319 | 0.818852 | 1.58E-52 | -1.69E-46 |
| MS.gene058908 | MS.gene28751 | 0.805972 | 1.03E-49 | -1.69E-46 |
| MS.gene058908 | MS.gene28944 | 0.804026 | 2.63E-49 | -1.69E-46 |
| MS.gene058908 | MS.gene31468 | 0.821007 | 5.08E-53 | -1.69E-46 |
| MS.gene058908 | MS.gene33131 | 0.8014 | 9.17E-49 | -1.69E-46 |
| MS.gene058908 | MS.gene33935 | -0.80191 | 7.20E-49 | -1.69E-46 |
| MS.gene058908 | MS.gene35135 | 0.801427 | 9.05E-49 | -1.69E-46 |
| MS.gene058908 | MS.gene39207 | 0.809421 | 1.91E-50 | -1.69E-46 |
| MS.gene058908 | MS.gene40513 | 0.80101 | 1.10E-48 | -1.69E-46 |
| MS.gene058908 | MS.gene40811 | 0.813825 | 2.10E-51 | -1.69E-46 |
| MS.gene058908 | MS.gene43970 | 0.809163 | 2.17E-50 | -1.69E-46 |
| MS.gene058908 | MS.gene44165 | 0.804267 | 2.34E-49 | -1.69E-46 |
| MS.gene058908 | MS.gene47957 | 0.998914 | 1.19E-281 | -1.69E-46 |
| MS.gene058908 | MS.gene47959 | 0.986014 | 2.16E-165 | -1.69E-46 |
| MS.gene058908 | MS.gene47962 | 0.85512 | 7.81E-62 | -1.69E-46 |
| MS.gene058908 | MS.gene55732 | 0.802597 | 5.20E-49 | -1.69E-46 |
| MS.gene058908 | MS.gene56253 | 0.820976 | 5.16E-53 | -1.69E-46 |
| MS.gene058908 | MS.gene60781 | -0.805959 | 1.04E-49 | -1.69E-46 |
| MS.gene058908 | MS.gene61288 | 0.805975 | 1.03E-49 | -1.69E-46 |
| MS.gene058908 | MS.gene62558 | 0.81904 | 1.43E-52 | -1.69E-46 |
| MS.gene058908 | MS.gene62563 | 0.820398 | 7.00E-53 | -1.69E-46 |
| MS.gene058908 | MS.gene63809 | 0.80814 | 3.59E-50 | -1.69E-46 |
| MS.gene058908 | MS.gene63811 | 0.827984 | 1.16E-54 | -1.69E-46 |
| MS.gene058908 | MS.gene66339 | 0.80901 | 2.34E-50 | -1.69E-46 |
| MS.gene058908 | MS.gene71825 | 0.831057 | 2.07E-55 | -1.69E-46 |
| MS.gene058908 | MS.gene71826 | 0.819975 | 8.76E-53 | -1.69E-46 |
| MS.gene058908 | MS.gene71949 | 0.807783 | 4.27E-50 | -1.69E-46 |
| MS.gene058908 | MS.gene71988 | 0.81875 | 1.67E-52 | -1.69E-46 |
| MS.gene058908 | MS.gene72347 | 0.812304 | 4.54E-51 | -1.69E-46 |
| MS.gene058908 | MS.gene74503 | 0.841999 | 3.38E-58 | -1.69E-46 |
| MS.gene058908 | MS.gene75060 | 0.819763 | 9.79E-53 | -1.69E-46 |
| MS.gene058908 | MS.gene75062 | 0.818771 | 1.65E-52 | -1.69E-46 |
| MS.gene058908 | MS.gene75187 | 0.818659 | 1.75E-52 | -1.69E-46 |
| MS.gene058908 | MS.gene77588 | 0.811668 | 6.24E-51 | -1.69E-46 |
| MS.gene058908 | MS.gene88495 | 0.800113 | 1.68E-48 | -1.69E-46 |
| MS.gene058908 | MS.gene89721 | 0.812646 | 3.82E-51 | -1.69E-46 |
| MS.gene058908 | MS.gene91310 | 0.804663 | 1.94E-49 | -1.69E-46 |
| MS.gene058908 | MS.gene91622 | 0.817219 | 3.69E-52 | -1.69E-46 |
| MS.gene058908 | MS.gene92186 | 0.803777 | 2.97E-49 | -1.69E-46 |
| MS.gene058908 | MS.gene97008 | 0.80281 | 4.70E-49 | -1.69E-46 |
| MS.gene058908 | MS.gene98436 | 0.816175 | 6.33E-52 | -1.69E-46 |
| MS.gene058908 | MS.gene99657 | 0.803765 | 2.98E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058908.t1 | MTR_8g089655 | 98.077 | 104 | 2 | 0 | 1 | 104 | 43 | 146 | 1.53e-71 | 209 |
| MS.gene058908.t1 | MTR_8g089655 | 98.077 | 104 | 2 | 0 | 1 | 104 | 40 | 143 | 1.89e-71 | 209 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058908.t1 | AT1G16790 | 55.670 | 97 | 40 | 2 | 1 | 95 | 39 | 134 | 2.72e-33 | 113 |
Find 0 sgRNAs with CRISPR-Local
Find 24 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATGAATCTTCTAGAAAGA+TGG | + | 57395:20649-20668 | MS.gene058908:CDS | 25.0% |
| TTCATCTGGTAAAGGTAAAA+AGG | + | 57395:20616-20635 | MS.gene058908:CDS | 30.0% | |
| ! | ATCTTCTAGAAAGATGGATT+CGG | + | 57395:20655-20674 | MS.gene058908:CDS | 30.0% |
| ! | TTGTTTGGAGTAATTTCATC+TGG | + | 57395:20602-20621 | MS.gene058908:CDS | 30.0% |
| CTGGTAAAGGTAAAAAGGAT+AGG | + | 57395:20621-20640 | MS.gene058908:CDS | 35.0% | |
| GAATTCCAAATCTGAGCAAT+AGG | - | 57395:20812-20831 | None:intergenic | 35.0% | |
| GGAGTAATTTCATCTGGTAA+AGG | + | 57395:20608-20627 | MS.gene058908:CDS | 35.0% | |
| TTGTTGAGACGTTTGCAATT+GGG | + | 57395:20729-20748 | MS.gene058908:CDS | 35.0% | |
| TTTCACCTATTGCTCAGATT+TGG | + | 57395:20804-20823 | MS.gene058908:CDS | 35.0% | |
| TTTGTTGAGACGTTTGCAAT+TGG | + | 57395:20728-20747 | MS.gene058908:CDS | 35.0% | |
| ATCCTCTATGACCTTCAATG+AGG | - | 57395:20570-20589 | None:intergenic | 40.0% | |
| ATGAAGCTTGATACTAAGCC+TGG | + | 57395:20704-20723 | MS.gene058908:CDS | 40.0% | |
| GCAATAGGTGAAAGAATGTG+AGG | - | 57395:20797-20816 | None:intergenic | 40.0% | |
| GGTCATAGAGGATTTACAGA+AGG | + | 57395:20577-20596 | MS.gene058908:CDS | 40.0% | |
| TCATTGAGGGGTAGATTTAG+TGG | - | 57395:20846-20865 | None:intergenic | 40.0% | |
| TGTTGAGACGTTTGCAATTG+GGG | + | 57395:20730-20749 | MS.gene058908:CDS | 40.0% | |
| TTCACAAGAAGCCTCATTGA+AGG | + | 57395:20556-20575 | MS.gene058908:CDS | 40.0% | |
| ! | TTTGCAATTGGGGTTGTTTC+TGG | + | 57395:20740-20759 | MS.gene058908:CDS | 40.0% |
| !! | GATTTACAGAAGGCGTTGTT+TGG | + | 57395:20587-20606 | MS.gene058908:CDS | 40.0% |
| AGCCTCATTGAAGGTCATAG+AGG | + | 57395:20565-20584 | MS.gene058908:CDS | 45.0% | |
| GCAAACGTCTCAACAAATCC+AGG | - | 57395:20725-20744 | None:intergenic | 45.0% | |
| !! | GGTGCTGCTCTTAATGGAAT+TGG | + | 57395:20761-20780 | MS.gene058908:CDS | 45.0% |
| !! | GTTTCTGGTGCTGCTCTTAA+TGG | + | 57395:20755-20774 | MS.gene058908:CDS | 45.0% |
| AGGGGTAGATTTAGTGGCTG+AGG | - | 57395:20840-20859 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 57395 | gene | 20551 | 20865 | 20551 | ID=MS.gene058908 |
| 57395 | mRNA | 20551 | 20865 | 20551 | ID=MS.gene058908.t1;Parent=MS.gene058908 |
| 57395 | exon | 20551 | 20865 | 20551 | ID=MS.gene058908.t1.exon1;Parent=MS.gene058908.t1 |
| 57395 | CDS | 20551 | 20865 | 20551 | ID=cds.MS.gene058908.t1;Parent=MS.gene058908.t1 |
| Gene Sequence |
| Protein sequence |
