AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene63856


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene63856.t1 MTR_5g094340 93.046 417 28 1 1 416 1 417 0.0 766
MS.gene63856.t1 MTR_3g055960 71.560 436 98 8 1 416 3 432 0.0 591
MS.gene63856.t1 MTR_5g089720 38.795 415 194 10 53 416 32 437 1.01e-84 265
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene63856.t1 AT5G55530 55.607 428 155 12 1 416 1 405 2.21e-145 419
MS.gene63856.t1 AT5G55530 55.607 428 155 12 1 416 1 405 2.21e-145 419
MS.gene63856.t1 AT5G55530 55.607 428 155 12 1 416 1 405 2.21e-145 419
MS.gene63856.t1 AT1G50570 51.765 425 159 11 1 416 1 388 2.42e-125 368
MS.gene63856.t1 AT1G50570 51.765 425 159 11 1 416 1 388 2.42e-125 368
MS.gene63856.t1 AT5G12300 47.000 200 97 4 54 247 19 215 6.34e-53 181

Find 71 sgRNAs with CRISPR-Local

Find 86 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTAAATGAAGACCACTTAA+TGG 0.202475 5.1:+75602654 MS.gene63856:CDS
TCCAATCAAGAGCAAATTTC+AGG 0.248354 5.1:+75602883 MS.gene63856:CDS
TTCACACTCACAACAGGTTT+TGG 0.272566 5.1:-75602979 None:intergenic
TCTAATCCAGAGGAATTTAT+TGG 0.280624 5.1:+75602109 MS.gene63856:CDS
TAGCATAAACATCTTGCTTA+TGG 0.294667 5.1:-75602186 None:intergenic
GAAAATCTTAGTTCAGAAAA+AGG 0.307362 5.1:+75602721 MS.gene63856:CDS
TTTCTGTCCAATTCAGTATT+AGG 0.324501 5.1:-75602553 None:intergenic
ATATCCTGCTGCACCATCTT+TGG 0.325154 5.1:-75603015 None:intergenic
TCCTCCACCATTGATAATCT+TGG 0.340540 5.1:-75602248 None:intergenic
ACCTGAAATTTGCTCTTGAT+TGG 0.358532 5.1:-75602884 None:intergenic
GAAAGTGGACCAACTAGTTC+AGG 0.360152 5.1:+75603111 MS.gene63856:CDS
GCCATTCAAGAGTTCTATCT+TGG 0.368720 5.1:+75601991 MS.gene63856:CDS
AGCAATGCCTAATACTGAAT+TGG 0.385474 5.1:+75602546 MS.gene63856:CDS
TGCTGCACCATCTTTGGCTC+TGG 0.397036 5.1:-75603009 None:intergenic
TGGCGCTTCACCCGATGTTA+TGG 0.403343 5.1:+75602516 MS.gene63856:CDS
ATAACCTTCGTCTAAGTGTT+AGG 0.404097 5.1:+75602290 MS.gene63856:CDS
TCTAAAGACATTGAGGTCAA+TGG 0.413522 5.1:+75602067 MS.gene63856:CDS
GTCAATACCAAGATTATCAA+TGG 0.416963 5.1:+75602241 MS.gene63856:CDS
TCAATATTCACACTCACAAC+AGG 0.440373 5.1:-75602985 None:intergenic
GATGGTGCAGCAGGATATAG+TGG 0.440448 5.1:+75603020 MS.gene63856:CDS
GTGAGATCTGGATGCTTAGC+AGG 0.442573 5.1:+75602335 MS.gene63856:CDS
CTTCTCTCAAATGTGAGATC+TGG 0.446006 5.1:+75602323 MS.gene63856:CDS
TATCTTGAAGACCAATTGCT+TGG 0.469813 5.1:+75602367 MS.gene63856:CDS
TCTGAAGTATTGATGAAAGA+TGG 0.470375 5.1:+75602409 MS.gene63856:CDS
CTCATTCAGCAACGTATTCG+AGG 0.473738 5.1:-75602950 None:intergenic
TGAATCTGTTCAACCTCCTA+AGG 0.483694 5.1:+75602780 MS.gene63856:CDS
GAACAAAACCTGCTGGTGAA+TGG 0.486115 5.1:-75602474 None:intergenic
ATGTTTACATATATCAAGCT+AGG 0.486965 5.1:+75602140 MS.gene63856:CDS
CCAAAGCAAAACCAAGCAAT+TGG 0.489137 5.1:-75602378 None:intergenic
CTAGGTGGAGATGCAACCTT+AGG 0.492862 5.1:-75602796 None:intergenic
ATACTTTGGTACTGATAGCT+TGG 0.493813 5.1:+75602684 MS.gene63856:CDS
AGAGCCAAAGATGGTGCAGC+AGG 0.494831 5.1:+75603011 MS.gene63856:CDS
GTTGAGTTTCCTGAACTAGT+TGG 0.500780 5.1:-75603120 None:intergenic
ACTACTCCAATAAATTCCTC+TGG 0.500999 5.1:-75602115 None:intergenic
GATCTATTCCATTCACCAGC+AGG 0.502121 5.1:+75602466 MS.gene63856:CDS
TCCTTTGTCTAAAGACATTG+AGG 0.512174 5.1:+75602060 MS.gene63856:CDS
CCATTCAAGAGTTCTATCTT+GGG 0.515014 5.1:+75601992 MS.gene63856:CDS
CTTCCTATGGACTTTGAAAG+TGG 0.518348 5.1:+75603096 MS.gene63856:CDS
GCTGAAATTGCCATAACATC+GGG 0.522915 5.1:-75602526 None:intergenic
GCATCTCCACCTAGCAGTAA+TGG 0.534582 5.1:+75602805 MS.gene63856:CDS
TTAATGGTTTCAGAATACTT+TGG 0.535977 5.1:+75602670 MS.gene63856:CDS
AGTATTCTGAAACCATTAAG+TGG 0.538501 5.1:-75602666 None:intergenic
GGTGGAGATGCAACCTTAGG+AGG 0.554345 5.1:-75602793 None:intergenic
AATACTGAATTGGACAGAAA+CGG 0.555790 5.1:+75602556 MS.gene63856:CDS
ACCAAGATTATCAATGGTGG+AGG 0.556066 5.1:+75602247 MS.gene63856:CDS
TGGTGCTGTTTCCTCTTCAG+TGG 0.556475 5.1:+75602825 MS.gene63856:CDS
ACATTTGAGAGAAGAATCAA+TGG 0.559219 5.1:-75602314 None:intergenic
CAATTGTCACTTGCTTACAA+TGG 0.566767 5.1:+75602496 MS.gene63856:CDS
AGATTGTAGTTTCTGTTCAG+AGG 0.567533 5.1:-75603142 None:intergenic
AATGGCGACACAACAGATTG+TGG 0.569154 5.1:-75601974 None:intergenic
CCCAAGATAGAACTCTTGAA+TGG 0.571896 5.1:-75601992 None:intergenic
TGCTGAAATTGCCATAACAT+CGG 0.572348 5.1:-75602527 None:intergenic
TGTGCTTGCAGCCACTGAAG+AGG 0.573218 5.1:-75602836 None:intergenic
GGTCCACTTTCAAAGTCCAT+AGG 0.582924 5.1:-75603099 None:intergenic
CGAAGGTTATCGTTAAACAC+GGG 0.594871 5.1:-75602277 None:intergenic
TTCATCAATACTTCAGACAA+AGG 0.596605 5.1:-75602403 None:intergenic
AAAAGAGTTGATCTCAAAGA+CGG 0.598346 5.1:+75602916 MS.gene63856:CDS
TGTTTACATATATCAAGCTA+GGG 0.599701 5.1:+75602141 MS.gene63856:CDS
AAGAGTTCTATCTTGGGACA+TGG 0.602172 5.1:+75601998 MS.gene63856:CDS
ACCTCAATGTCTTTAGACAA+AGG 0.602535 5.1:-75602061 None:intergenic
TATTGAGCCAGAGCCAAAGA+TGG 0.602654 5.1:+75603002 MS.gene63856:CDS
TGCTGGTGAATGGAATAGAT+CGG 0.604836 5.1:-75602464 None:intergenic
AATACCAAGATTATCAATGG+TGG 0.611628 5.1:+75602244 MS.gene63856:CDS
GACAATTGAACAAAACCTGC+TGG 0.612847 5.1:-75602481 None:intergenic
GAAACAGCACCATTACTGCT+AGG 0.628325 5.1:-75602814 None:intergenic
TGGTCCTAACACTTAGACGA+AGG 0.635127 5.1:-75602294 None:intergenic
ACGAAGGTTATCGTTAAACA+CGG 0.658274 5.1:-75602278 None:intergenic
TGTTGCAGTGTCTAATCCAG+AGG 0.663929 5.1:+75602099 MS.gene63856:CDS
TGAGATCTGGATGCTTAGCA+GGG 0.697112 5.1:+75602336 MS.gene63856:CDS
ACAGCACCATTACTGCTAGG+TGG 0.706083 5.1:-75602811 None:intergenic
GAAGGTTATCGTTAAACACG+GGG 0.724753 5.1:-75602276 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CATTTACAATTTTTGGATTT+GGG - chr5.1:75602642-75602661 None:intergenic 20.0%
!!! TCATTTACAATTTTTGGATT+TGG - chr5.1:75602643-75602662 None:intergenic 20.0%
! ATGTTTACATATATCAAGCT+AGG + chr5.1:75602140-75602159 MS.gene63856:CDS 25.0%
! GAAAATCTTAGTTCAGAAAA+AGG + chr5.1:75602721-75602740 MS.gene63856:CDS 25.0%
! TGTTTACATATATCAAGCTA+GGG + chr5.1:75602141-75602160 MS.gene63856:CDS 25.0%
! TTAATGGTTTCAGAATACTT+TGG + chr5.1:75602670-75602689 MS.gene63856:CDS 25.0%
!! TGGTCTTCATTTACAATTTT+TGG - chr5.1:75602649-75602668 None:intergenic 25.0%
!!! TTGGTATTGACAAAATTTTC+AGG - chr5.1:75602232-75602251 None:intergenic 25.0%
AATACCAAGATTATCAATGG+TGG + chr5.1:75602244-75602263 MS.gene63856:CDS 30.0%
AATACTGAATTGGACAGAAA+CGG + chr5.1:75602556-75602575 MS.gene63856:CDS 30.0%
ACATTTGAGAGAAGAATCAA+TGG - chr5.1:75602317-75602336 None:intergenic 30.0%
GTCAATACCAAGATTATCAA+TGG + chr5.1:75602241-75602260 MS.gene63856:CDS 30.0%
TAGCATAAACATCTTGCTTA+TGG - chr5.1:75602189-75602208 None:intergenic 30.0%
TCTAATCCAGAGGAATTTAT+TGG + chr5.1:75602109-75602128 MS.gene63856:CDS 30.0%
TGTAAATGAAGACCACTTAA+TGG + chr5.1:75602654-75602673 MS.gene63856:CDS 30.0%
TTCATCAATACTTCAGACAA+AGG - chr5.1:75602406-75602425 None:intergenic 30.0%
TTTCTGTCCAATTCAGTATT+AGG - chr5.1:75602556-75602575 None:intergenic 30.0%
! AAAAGAGTTGATCTCAAAGA+CGG + chr5.1:75602916-75602935 MS.gene63856:CDS 30.0%
! AAGATTTTCAGTATCAGTAG+TGG - chr5.1:75602710-75602729 None:intergenic 30.0%
!! AGTATTCTGAAACCATTAAG+TGG - chr5.1:75602669-75602688 None:intergenic 30.0%
!! CTTCATTTTTGCTAATGACT+CGG - chr5.1:75603076-75603095 None:intergenic 30.0%
!! TCTGAAGTATTGATGAAAGA+TGG + chr5.1:75602409-75602428 MS.gene63856:CDS 30.0%
ACCTCAATGTCTTTAGACAA+AGG - chr5.1:75602064-75602083 None:intergenic 35.0%
ACGAAGGTTATCGTTAAACA+CGG - chr5.1:75602281-75602300 None:intergenic 35.0%
ACTACTCCAATAAATTCCTC+TGG - chr5.1:75602118-75602137 None:intergenic 35.0%
AGATTGTAGTTTCTGTTCAG+AGG - chr5.1:75603145-75603164 None:intergenic 35.0%
AGCAAAAATGAAGCTTCCTA+TGG + chr5.1:75603083-75603102 MS.gene63856:CDS 35.0%
AGCAATGCCTAATACTGAAT+TGG + chr5.1:75602546-75602565 MS.gene63856:CDS 35.0%
ATAACCTTCGTCTAAGTGTT+AGG + chr5.1:75602290-75602309 MS.gene63856:CDS 35.0%
CCATTCAAGAGTTCTATCTT+GGG + chr5.1:75601992-75602011 MS.gene63856:CDS 35.0%
TATCTTGAAGACCAATTGCT+TGG + chr5.1:75602367-75602386 MS.gene63856:CDS 35.0%
TCAATATTCACACTCACAAC+AGG - chr5.1:75602988-75603007 None:intergenic 35.0%
TCCAATCAAGAGCAAATTTC+AGG + chr5.1:75602883-75602902 MS.gene63856:CDS 35.0%
TCCTTTGTCTAAAGACATTG+AGG + chr5.1:75602060-75602079 MS.gene63856:CDS 35.0%
TCTAAAGACATTGAGGTCAA+TGG + chr5.1:75602067-75602086 MS.gene63856:CDS 35.0%
TGCTGAAATTGCCATAACAT+CGG - chr5.1:75602530-75602549 None:intergenic 35.0%
! ACCTGAAATTTGCTCTTGAT+TGG - chr5.1:75602887-75602906 None:intergenic 35.0%
! AGATCAACTCTTTTCTCTTC+TGG - chr5.1:75602910-75602929 None:intergenic 35.0%
! AGTAACTCACGTGTGTTTTA+TGG + chr5.1:75603174-75603193 MS.gene63856:CDS 35.0%
! CAATTGTCACTTGCTTACAA+TGG + chr5.1:75602496-75602515 MS.gene63856:CDS 35.0%
!! AGAAAAAGGTGTTCAACTTG+TGG + chr5.1:75602735-75602754 MS.gene63856:CDS 35.0%
!! ATACTTTGGTACTGATAGCT+TGG + chr5.1:75602684-75602703 MS.gene63856:CDS 35.0%
AAGAGTTCTATCTTGGGACA+TGG + chr5.1:75601998-75602017 MS.gene63856:CDS 40.0%
ACCAAGATTATCAATGGTGG+AGG + chr5.1:75602247-75602266 MS.gene63856:CDS 40.0%
CCAAAGCAAAACCAAGCAAT+TGG - chr5.1:75602381-75602400 None:intergenic 40.0%
CCCAAGATAGAACTCTTGAA+TGG - chr5.1:75601995-75602014 None:intergenic 40.0%
CGAAGGTTATCGTTAAACAC+GGG - chr5.1:75602280-75602299 None:intergenic 40.0%
CTTCTCTCAAATGTGAGATC+TGG + chr5.1:75602323-75602342 MS.gene63856:CDS 40.0%
GAAGGTTATCGTTAAACACG+GGG - chr5.1:75602279-75602298 None:intergenic 40.0%
GACAATTGAACAAAACCTGC+TGG - chr5.1:75602484-75602503 None:intergenic 40.0%
GCCATTCAAGAGTTCTATCT+TGG + chr5.1:75601991-75602010 MS.gene63856:CDS 40.0%
GCTGAAATTGCCATAACATC+GGG - chr5.1:75602529-75602548 None:intergenic 40.0%
GTTGAGTTTCCTGAACTAGT+TGG - chr5.1:75603123-75603142 None:intergenic 40.0%
TGAATCTGTTCAACCTCCTA+AGG + chr5.1:75602780-75602799 MS.gene63856:CDS 40.0%
TGCTGGTGAATGGAATAGAT+CGG - chr5.1:75602467-75602486 None:intergenic 40.0%
! CTTCCTATGGACTTTGAAAG+TGG + chr5.1:75603096-75603115 MS.gene63856:CDS 40.0%
! TCCTCCACCATTGATAATCT+TGG - chr5.1:75602251-75602270 None:intergenic 40.0%
! TTCACACTCACAACAGGTTT+TGG - chr5.1:75602982-75603001 None:intergenic 40.0%
!!! CCAATTGCTTGGTTTTGCTT+TGG + chr5.1:75602378-75602397 MS.gene63856:CDS 40.0%
!!! GCTTTTTTCGGAGTCTTTCT+TGG - chr5.1:75602584-75602603 None:intergenic 40.0%
AATGGCGACACAACAGATTG+TGG - chr5.1:75601977-75601996 None:intergenic 45.0%
ATATCCTGCTGCACCATCTT+TGG - chr5.1:75603018-75603037 None:intergenic 45.0%
CTCATTCAGCAACGTATTCG+AGG - chr5.1:75602953-75602972 None:intergenic 45.0%
GAAACAGCACCATTACTGCT+AGG - chr5.1:75602817-75602836 None:intergenic 45.0%
GAAAGTGGACCAACTAGTTC+AGG + chr5.1:75603111-75603130 MS.gene63856:CDS 45.0%
GAACAAAACCTGCTGGTGAA+TGG - chr5.1:75602477-75602496 None:intergenic 45.0%
GATCTATTCCATTCACCAGC+AGG + chr5.1:75602466-75602485 MS.gene63856:CDS 45.0%
GGTCCACTTTCAAAGTCCAT+AGG - chr5.1:75603102-75603121 None:intergenic 45.0%
TATTGAGCCAGAGCCAAAGA+TGG + chr5.1:75603002-75603021 MS.gene63856:CDS 45.0%
TGAGATCTGGATGCTTAGCA+GGG + chr5.1:75602336-75602355 MS.gene63856:CDS 45.0%
TGGTCCTAACACTTAGACGA+AGG - chr5.1:75602297-75602316 None:intergenic 45.0%
TGTTGCAGTGTCTAATCCAG+AGG + chr5.1:75602099-75602118 MS.gene63856:CDS 45.0%
AAAAAAGCGAGTCGCTCGTG+AGG + chr5.1:75602596-75602615 MS.gene63856:CDS 50.0%
AAAAAGCGAGTCGCTCGTGA+GGG + chr5.1:75602597-75602616 MS.gene63856:CDS 50.0%
ACAGCACCATTACTGCTAGG+TGG - chr5.1:75602814-75602833 None:intergenic 50.0%
CTAGGTGGAGATGCAACCTT+AGG - chr5.1:75602799-75602818 None:intergenic 50.0%
GATGGTGCAGCAGGATATAG+TGG + chr5.1:75603020-75603039 MS.gene63856:CDS 50.0%
GCATCTCCACCTAGCAGTAA+TGG + chr5.1:75602805-75602824 MS.gene63856:CDS 50.0%
GTGAGATCTGGATGCTTAGC+AGG + chr5.1:75602335-75602354 MS.gene63856:CDS 50.0%
!! TGGTGCTGTTTCCTCTTCAG+TGG + chr5.1:75602825-75602844 MS.gene63856:CDS 50.0%
!!! CACGAGCGACTCGCTTTTTT+CGG - chr5.1:75602596-75602615 None:intergenic 50.0%
AGAGCCAAAGATGGTGCAGC+AGG + chr5.1:75603011-75603030 MS.gene63856:CDS 55.0%
GGTGGAGATGCAACCTTAGG+AGG - chr5.1:75602796-75602815 None:intergenic 55.0%
TGGCGCTTCACCCGATGTTA+TGG + chr5.1:75602516-75602535 MS.gene63856:CDS 55.0%
TGTGCTTGCAGCCACTGAAG+AGG - chr5.1:75602839-75602858 None:intergenic 55.0%
! TGCTGCACCATCTTTGGCTC+TGG - chr5.1:75603012-75603031 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 75601965 75603215 75601965 ID=MS.gene63856
chr5.1 mRNA 75601965 75603215 75601965 ID=MS.gene63856.t1;Parent=MS.gene63856
chr5.1 exon 75601965 75603215 75601965 ID=MS.gene63856.t1.exon1;Parent=MS.gene63856.t1
chr5.1 CDS 75601965 75603215 75601965 ID=cds.MS.gene63856.t1;Parent=MS.gene63856.t1
Gene Sequence

>MS.gene63856

ATGGAATCTCCACAATCTGTTGTGTCGCCATTCAAGAGTTCTATCTTGGGACATGGTGAGAAGTATAATTCTGATGTTTTAACACAAAGCTCTAGTCCTTTGTCTAAAGACATTGAGGTCAATGGAAAAGAAGCTGTTGCAGTGTCTAATCCAGAGGAATTTATTGGAGTAGTTGATGTTTACATATATCAAGCTAGGGACATTCATAACATCTGCATATACCATAAGCAAGATGTTTATGCTAAGATTTCTTTGACTAGTGATCCTGAAAATTTTGTCAATACCAAGATTATCAATGGTGGAGGAAGAAACCCCGTGTTTAACGATAACCTTCGTCTAAGTGTTAGGACCATTGATTCTTCTCTCAAATGTGAGATCTGGATGCTTAGCAGGGTGAAGAATTATCTTGAAGACCAATTGCTTGGTTTTGCTTTGGTACCTTTGTCTGAAGTATTGATGAAAGATGGAAAACTAGAGAAAGAGTTCTCTCTTTCTTCGACCGATCTATTCCATTCACCAGCAGGTTTTGTTCAATTGTCACTTGCTTACAATGGCGCTTCACCCGATGTTATGGCAATTTCAGCAATGCCTAATACTGAATTGGACAGAAACGGCACCAAGAAAGACTCCGAAAAAAGCGAGTCGCTCGTGAGGGATTTAGATAAAATAGAGTTCCCAAATCCAAAAATTGTAAATGAAGACCACTTAATGGTTTCAGAATACTTTGGTACTGATAGCTTGGCCACTACTGATACTGAAAATCTTAGTTCAGAAAAAGGTGTTCAACTTGTGGAAAGCTTCTCAGCATGTAGTGTTGAATCTGTTCAACCTCCTAAGGTTGCATCTCCACCTAGCAGTAATGGTGCTGTTTCCTCTTCAGTGGCTGCAAGCACAGAATCACATGCTGCTACTAAATCTTCCAATCAAGAGCAAATTTCAGGTCCAGAAGAGAAAAGAGTTGATCTCAAAGACGGCGAGAGTGATTCCTCGAATACGTTGCTGAATGAGTCATTTCCAAAACCTGTTGTGAGTGTGAATATTGAGCCAGAGCCAAAGATGGTGCAGCAGGATATAGTGGACATGTACTTGAAAAGTATGCAGCAATTTACCGAGTCATTAGCAAAAATGAAGCTTCCTATGGACTTTGAAAGTGGACCAACTAGTTCAGGAAACTCAACCTCTGAACAGAAACTACAATCTTCAAAGAGTAGTAACTCACGTGTGTTTTATGGTAGCAGAGCTTTCTTCTGA

Protein sequence

>MS.gene63856.t1

MESPQSVVSPFKSSILGHGEKYNSDVLTQSSSPLSKDIEVNGKEAVAVSNPEEFIGVVDVYIYQARDIHNICIYHKQDVYAKISLTSDPENFVNTKIINGGGRNPVFNDNLRLSVRTIDSSLKCEIWMLSRVKNYLEDQLLGFALVPLSEVLMKDGKLEKEFSLSSTDLFHSPAGFVQLSLAYNGASPDVMAISAMPNTELDRNGTKKDSEKSESLVRDLDKIEFPNPKIVNEDHLMVSEYFGTDSLATTDTENLSSEKGVQLVESFSACSVESVQPPKVASPPSSNGAVSSSVAASTESHAATKSSNQEQISGPEEKRVDLKDGESDSSNTLLNESFPKPVVSVNIEPEPKMVQQDIVDMYLKSMQQFTESLAKMKLPMDFESGPTSSGNSTSEQKLQSSKSSNSRVFYGSRAFF