AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene63933

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene63933.t1 MTR_0402s0040 63.500 200 64 2 12 203 2 200 1.02e-74 251
MS.gene63933.t1 MTR_0402s0040 50.175 285 72 3 388 672 200 414 1.77e-70 239
MS.gene63933.t1 MTR_5g015480 66.667 165 51 1 1 165 25 185 1.96e-65 218
MS.gene63933.t1 MTR_6g017200 72.727 121 33 0 12 132 34 154 1.23e-52 179
MS.gene63933.t1 MTR_2g070590 71.552 116 33 0 24 139 5 120 8.93e-51 173
MS.gene63933.t1 MTR_0137s0040 93.182 88 6 0 1 88 1 88 2.14e-50 172
MS.gene63933.t1 MTR_1g057920 68.800 125 37 1 1 125 1 123 1.57e-49 170
MS.gene63933.t1 MTR_7g113010 57.500 160 52 2 1 160 424 567 5.98e-46 173
MS.gene63933.t1 MTR_1g086270 76.543 81 19 0 323 403 1 81 4.03e-39 140
MS.gene63933.t1 MTR_6g051425 30.272 294 175 7 43 309 24 314 5.74e-34 133
MS.gene63933.t1 MTR_0558s0020 32.389 247 140 6 50 269 50 296 1.04e-32 129
MS.gene63933.t1 MTR_8g446020 34.091 176 107 2 103 269 81 256 1.02e-26 110
MS.gene63933.t1 MTR_1g070355 32.365 241 149 6 35 263 71 309 2.46e-26 114
MS.gene63933.t1 MTR_6g090505 35.838 173 102 2 105 268 58 230 9.35e-25 107
MS.gene63933.t1 MTR_0137s0070 72.973 74 19 1 683 755 557 630 2.29e-24 108
MS.gene63933.t1 MTR_0137s0070 73.973 73 18 1 684 755 593 665 3.19e-24 108
MS.gene63933.t1 MTR_0291s0060 65.753 73 25 0 14 86 13 85 3.28e-24 97.8
MS.gene63933.t1 MTR_3g014080 70.270 74 21 1 683 755 1139 1212 2.45e-22 103
MS.gene63933.t1 MTR_3g014080 71.233 73 20 1 684 755 1175 1247 3.06e-22 103
MS.gene63933.t1 MTR_1g050985 71.667 60 17 0 106 165 39 98 2.18e-21 90.1
MS.gene63933.t1 MTR_0137s0010 60.976 82 31 1 675 755 657 738 8.24e-21 98.2
MS.gene63933.t1 MTR_3g014040 60.976 82 31 1 675 755 1135 1216 1.38e-20 97.8
MS.gene63933.t1 MTR_5g039760 34.872 195 107 6 32 224 6 182 4.84e-20 89.0
MS.gene63933.t1 MTR_4g067290 41.481 135 66 5 36 163 56 184 7.24e-20 89.7
MS.gene63933.t1 MTR_4g067290 41.481 135 66 5 36 163 74 202 8.63e-20 90.1
MS.gene63933.t1 MTR_4g067290 41.481 135 66 5 36 163 76 204 8.64e-20 90.1
MS.gene63933.t1 MTR_4g067290 44.860 107 53 3 36 140 56 158 1.07e-19 89.4
MS.gene63933.t1 MTR_4g067290 44.860 107 53 3 36 140 76 178 1.84e-19 89.4
MS.gene63933.t1 MTR_4g067290 44.860 107 53 3 36 140 116 218 2.59e-19 89.7
MS.gene63933.t1 MTR_1g083640 27.197 239 142 7 34 240 44 282 5.62e-19 88.6
MS.gene63933.t1 MTR_7g074790 36.723 177 78 7 33 198 7 160 1.86e-18 85.5
MS.gene63933.t1 MTR_4g035820 27.178 287 183 9 8 273 43 324 3.40e-18 89.7
MS.gene63933.t1 MTR_2g098920 37.500 136 71 5 36 163 80 209 5.83e-18 85.1
MS.gene63933.t1 MTR_7g446520 28.571 245 149 6 39 274 91 318 2.63e-17 84.3
MS.gene63933.t1 MTR_3g007190 48.936 94 37 2 672 754 975 1068 8.50e-17 85.5
MS.gene63933.t1 MTR_1g091013 52.564 78 26 1 27 104 3 69 1.15e-16 75.5
MS.gene63933.t1 MTR_4g102720 29.070 172 86 3 33 204 4 139 1.54e-16 79.7
MS.gene63933.t1 MTR_7g061980 40.171 117 66 3 30 145 4 117 6.96e-16 75.1
MS.gene63933.t1 MTR_3g006370 38.136 118 66 3 36 152 50 161 6.41e-14 72.4
MS.gene63933.t1 MTR_5g045210 38.462 91 56 0 560 650 20 110 9.79e-14 70.9
MS.gene63933.t1 MTR_3g035815 54.688 64 28 1 685 747 57 120 2.95e-13 67.4
MS.gene63933.t1 MTR_0337s0040 28.000 150 99 2 96 236 75 224 7.34e-13 69.3
MS.gene63933.t1 MTR_3g032340 35.036 137 71 4 620 754 1172 1292 7.83e-13 72.8
MS.gene63933.t1 MTR_3g014320 50.633 79 37 2 678 754 1263 1341 9.30e-13 72.8
MS.gene63933.t1 MTR_2g099430 39.506 81 47 1 59 139 26 104 1.43e-12 65.5
MS.gene63933.t1 MTR_5g076070 24.779 226 158 5 42 257 59 282 2.20e-12 68.9
MS.gene63933.t1 MTR_1g019850 30.460 174 110 4 4 174 36 201 2.93e-12 67.8
MS.gene63933.t1 MTR_2g098880 36.607 112 62 3 36 142 61 168 5.18e-12 67.0
MS.gene63933.t1 MTR_2g098880 36.607 112 62 3 36 142 73 180 5.82e-12 67.4
MS.gene63933.t1 MTR_0337s0060 26.190 168 95 2 419 583 21 162 7.37e-12 66.6
MS.gene63933.t1 MTR_4g019360 53.030 66 30 1 684 748 1077 1142 8.96e-12 69.3
MS.gene63933.t1 MTR_3g435220 49.296 71 35 1 685 754 1125 1195 1.74e-11 68.6
MS.gene63933.t1 MTR_1g045960 47.059 85 39 2 676 754 1117 1201 2.04e-11 68.2
MS.gene63933.t1 MTR_7g446480 28.977 176 114 3 112 277 140 314 2.12e-11 66.2
MS.gene63933.t1 MTR_7g446510 28.409 176 115 3 112 277 155 329 3.14e-11 65.9
MS.gene63933.t1 MTR_3g021100 54.688 64 28 1 685 747 1084 1147 4.65e-11 67.0
MS.gene63933.t1 MTR_3g022400 56.452 62 26 1 685 745 1045 1106 5.55e-11 66.6
MS.gene63933.t1 MTR_3g021380 53.846 65 29 1 685 748 1066 1130 5.59e-11 66.6
MS.gene63933.t1 MTR_3g032110 35.252 139 66 5 620 754 1169 1287 5.90e-11 66.6
MS.gene63933.t1 MTR_3g022400 56.452 62 26 1 685 745 1073 1134 6.10e-11 66.6
MS.gene63933.t1 MTR_3g033860 29.940 167 104 3 590 755 1098 1252 6.59e-11 66.6
MS.gene63933.t1 MTR_6g022070 27.273 176 119 3 323 496 1 169 7.21e-11 64.7
MS.gene63933.t1 MTR_3g435210 38.017 121 59 4 626 745 823 928 9.63e-11 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene63933.t1 AT4G38180 33.988 662 415 9 7 649 42 700 1.26e-115 367
MS.gene63933.t1 AT4G15090 30.062 642 424 9 18 645 36 666 2.25e-92 307
MS.gene63933.t1 AT4G15090 30.062 642 424 9 18 645 36 666 2.25e-92 307
MS.gene63933.t1 AT4G15090 30.062 642 424 9 18 645 36 666 2.25e-92 307
MS.gene63933.t1 AT4G15090 30.062 642 424 9 18 645 36 666 2.25e-92 307
MS.gene63933.t1 AT4G15090 30.062 642 424 9 18 645 36 666 2.25e-92 307
MS.gene63933.t1 AT3G22170 31.703 634 374 15 24 628 54 657 2.29e-90 301
MS.gene63933.t1 AT3G22170 31.703 634 374 15 24 628 54 657 2.29e-90 301
MS.gene63933.t1 AT1G76320 31.553 618 384 15 40 640 1 596 4.17e-85 283
MS.gene63933.t1 AT1G76320 31.553 618 384 15 40 640 1 596 1.83e-84 283
MS.gene63933.t1 AT1G76320 31.553 618 384 15 40 640 1 596 2.11e-84 283
MS.gene63933.t1 AT1G76320 31.553 618 384 15 40 640 1 596 2.11e-84 283
MS.gene63933.t1 AT2G27110 29.584 649 415 13 11 652 30 643 2.74e-81 277
MS.gene63933.t1 AT2G27110 29.584 649 415 13 11 652 30 643 2.74e-81 277
MS.gene63933.t1 AT3G06250 28.943 615 381 14 36 640 190 758 5.97e-76 261
MS.gene63933.t1 AT3G06250 28.943 615 381 14 36 640 190 758 5.97e-76 261
MS.gene63933.t1 AT3G06250 28.943 615 381 14 36 640 190 758 5.97e-76 261
MS.gene63933.t1 AT5G18960 28.800 625 386 15 29 640 204 782 8.83e-72 250
MS.gene63933.t1 AT5G18960 30.921 152 89 3 24 173 31 168 3.90e-12 70.5
MS.gene63933.t1 AT5G18960 28.800 625 386 15 29 640 204 782 8.83e-72 250
MS.gene63933.t1 AT5G18960 30.921 152 89 3 24 173 31 168 3.90e-12 70.5
MS.gene63933.t1 AT2G32250 27.586 609 388 12 40 640 42 605 1.61e-71 249
MS.gene63933.t1 AT2G27110 32.671 453 289 8 207 652 55 498 2.78e-71 247
MS.gene63933.t1 AT2G32250 27.586 609 388 12 40 640 1 564 3.62e-71 248
MS.gene63933.t1 AT2G32250 27.586 609 388 12 40 640 1 564 3.68e-71 248
MS.gene63933.t1 AT2G32250 27.586 609 388 12 40 640 42 605 4.25e-71 248
MS.gene63933.t1 AT2G32250 27.586 609 388 12 40 640 42 605 4.25e-71 248
MS.gene63933.t1 AT2G32250 27.586 609 388 12 40 640 42 605 4.25e-71 248
MS.gene63933.t1 AT2G32250 27.586 609 388 12 40 640 42 605 4.25e-71 248
MS.gene63933.t1 AT2G32250 27.586 609 388 12 40 640 42 605 4.25e-71 248
MS.gene63933.t1 AT1G10240 28.130 583 386 14 17 580 36 604 1.42e-65 231
MS.gene63933.t1 AT1G10240 28.130 583 386 14 17 580 36 604 1.42e-65 231
MS.gene63933.t1 AT4G19990 28.367 698 422 17 24 682 15 673 1.51e-64 229
MS.gene63933.t1 AT4G19990 28.125 672 415 14 41 682 26 659 1.63e-64 229
MS.gene63933.t1 AT4G19990 28.125 672 415 14 41 682 26 659 1.65e-64 229
MS.gene63933.t1 AT4G19990 28.125 672 415 14 41 682 26 659 1.65e-64 229
MS.gene63933.t1 AT4G38170 28.195 493 325 9 212 692 27 502 2.30e-64 224
MS.gene63933.t1 AT4G19990 28.060 670 418 13 41 682 26 659 2.83e-64 229
MS.gene63933.t1 AT4G19990 28.060 670 418 13 41 682 26 659 2.83e-64 229
MS.gene63933.t1 AT4G19990 27.673 636 396 13 75 682 3 602 1.09e-56 206
MS.gene63933.t1 AT5G28530 26.131 597 402 13 34 603 53 637 2.62e-55 202
MS.gene63933.t1 AT5G28530 26.131 597 402 13 34 603 53 637 2.62e-55 202
MS.gene63933.t1 AT5G28530 25.667 600 405 12 34 603 53 641 6.48e-55 201
MS.gene63933.t1 AT1G80010 26.678 596 376 15 28 583 87 661 7.20e-54 199
MS.gene63933.t1 AT1G52520 28.448 580 355 17 35 581 84 636 7.37e-54 198
MS.gene63933.t1 AT5G28530 24.957 581 366 17 34 603 53 574 1.08e-37 150
MS.gene63933.t1 AT5G28530 24.486 584 369 16 34 603 53 578 2.46e-37 149
MS.gene63933.t1 AT3G59470 40.441 136 71 4 36 165 70 201 4.19e-20 90.9
MS.gene63933.t1 AT3G59470 40.441 136 71 4 36 165 70 201 4.19e-20 90.9
MS.gene63933.t1 AT3G59470 40.441 136 71 4 36 165 82 213 5.65e-20 90.9
MS.gene63933.t1 AT3G07500 36.429 140 85 3 1 140 1 136 2.21e-15 76.3
MS.gene63933.t1 AT2G43280 42.202 109 57 4 42 146 28 134 3.02e-14 72.8
MS.gene63933.t1 AT4G12850 33.077 130 81 3 36 163 11 136 3.19e-12 65.1
MS.gene63933.t1 AT4G12850 33.645 107 67 2 36 142 11 113 4.82e-12 64.7
MS.gene63933.t1 AT2G43280 40.000 100 54 4 51 146 2 99 2.00e-11 63.5
MS.gene63933.t1 AT4G12850 33.645 107 67 2 36 142 11 113 2.05e-11 63.9

Find 136 sgRNAs with CRISPR-Local

Find 222 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAAAATAACAAATGTATTT+TGG 0.095455 3.4:+10089245 MS.gene63933:CDS
TTCTCTACACCTTCAACTAT+TGG 0.269471 3.4:-10088575 None:intergenic
TTTGGTATTCGTCGAGAATA+TGG 0.287007 3.4:+10088737 MS.gene63933:CDS
CACAATCCATGAAATGTTTC+CGG 0.296465 3.4:-10089550 None:intergenic
GCACAAATGATAAACGATTA+TGG 0.303139 3.4:+10089274 MS.gene63933:CDS
TTGAACTCTCTTGATATAGA+TGG 0.314695 3.4:-10089612 None:intergenic
AGTTGAAGGTGTAGAGAAAT+TGG 0.324040 3.4:+10088580 MS.gene63933:CDS
AATAAGTATAGTAGGAGAAT+TGG 0.329631 3.4:+10088713 MS.gene63933:CDS
TTGCATGTTGATCAGTTAGA+AGG 0.329715 3.4:-10089498 None:intergenic
AATAAGGAGCGGACGGTAAT+GGG 0.340111 3.4:+10090132 MS.gene63933:CDS
CATTTCATGGATTGTGCACT+TGG 0.341154 3.4:+10089557 MS.gene63933:CDS
ATTGGGAAGTAATCAAGATT+TGG 0.341191 3.4:+10090305 MS.gene63933:CDS
ATAATGTTTCTGAAGATTCA+TGG 0.342573 3.4:+10089722 MS.gene63933:CDS
CGCCCGTTGGGAGTATTTGT+TGG 0.343808 3.4:+10089352 MS.gene63933:CDS
GCTGTAGCGGCTGACGAATC+TGG 0.349940 3.4:+10089037 MS.gene63933:CDS
ATTTCACTTGATCGAAAGAT+TGG 0.360362 3.4:+10088902 MS.gene63933:CDS
ACCTTGAGATTTCAGATTGT+CGG 0.364901 3.4:+10091457 MS.gene63933:CDS
GACGGTAATGGGGAATCTTA+TGG 0.365249 3.4:+10090143 MS.gene63933:CDS
TACAAATTATGATAATAATA+AGG 0.368461 3.4:+10090116 MS.gene63933:CDS
TTGATCAATTTAACCATTTG+AGG 0.371275 3.4:-10090390 None:intergenic
GGTGTGGATATTGTGCAAAA+TGG 0.375424 3.4:+10088614 MS.gene63933:CDS
CCCTCCTTGCTCTGACAGCT+TGG 0.376183 3.4:-10091556 None:intergenic
TCAAATAACCATTGAAAAGA+AGG 0.380370 3.4:-10089436 None:intergenic
TACGACATACATGACAAATA+AGG 0.385236 3.4:+10089324 MS.gene63933:CDS
CGTGCTTTAAAGAGGGTAAA+CGG 0.389596 3.4:+10088804 MS.gene63933:CDS
GAGAAATTGGTGAACAATAT+TGG 0.394648 3.4:+10088593 MS.gene63933:CDS
ACGCGCATCACATCCCGTTC+TGG 0.397818 3.4:-10088873 None:intergenic
GGCTATTTCTTTAGTTATGC+CGG 0.399841 3.4:+10089531 MS.gene63933:CDS
ATAAGTATAGTAGGAGAATT+GGG 0.404314 3.4:+10088714 MS.gene63933:CDS
GTGTGGATATTGTGCAAAAT+GGG 0.408834 3.4:+10088615 MS.gene63933:CDS
TCTGGATGTTGGTGGGGAGC+AGG 0.410929 3.4:+10090584 MS.gene63933:CDS
ATGGACAAGAGATGCAAGAT+TGG 0.411222 3.4:+10090287 MS.gene63933:CDS
GTTATGCCGGAAACATTTCA+TGG 0.413868 3.4:+10089544 MS.gene63933:CDS
TGCCTAAATTGAAAATGAAA+AGG 0.417192 3.4:+10089971 MS.gene63933:CDS
TTATACACCACACTTGGAAA+TGG 0.423013 3.4:+10088655 MS.gene63933:CDS
GTGCTTTAAAGAGGGTAAAC+GGG 0.424178 3.4:+10088805 MS.gene63933:CDS
GAATATGTGTACAAGCAAAA+TGG 0.424444 3.4:+10089082 MS.gene63933:CDS
GAGGAAGGCAGTCCTTCTTC+TGG 0.424904 3.4:-10091495 None:intergenic
GGAATTTCAAGAAGAATATG+AGG 0.425849 3.4:+10090044 MS.gene63933:CDS
TAATAAGGAGCGGACGGTAA+TGG 0.433456 3.4:+10090131 MS.gene63933:CDS
CTCATGTCTTGACTCATAAT+CGG 0.441200 3.4:-10089947 None:intergenic
GGAAGGGCAACCTATTGAAC+TGG 0.450560 3.4:+10090326 MS.gene63933:CDS
CTGGATGTTGGTGGGGAGCA+GGG 0.451018 3.4:+10090585 MS.gene63933:CDS
GGGGAATCTTATGGATCAAA+AGG 0.451072 3.4:+10090152 MS.gene63933:CDS
AACGGGATGTGATGCGCGTA+TGG 0.457812 3.4:+10088877 MS.gene63933:CDS
CATTGTTGATGCATTTCAAA+CGG 0.460056 3.4:+10089173 MS.gene63933:CDS
GAAGAATATGAGGAATATCT+AGG 0.460765 3.4:+10090054 MS.gene63933:CDS
TAAAATAATTGATGATATGT+TGG 0.463062 3.4:+10090473 MS.gene63933:CDS
TGCGTAAAGAATTTGAAAGA+AGG 0.475897 3.4:+10090081 MS.gene63933:CDS
TTGGTTATCTTCAAACTAGA+AGG 0.476088 3.4:+10089119 MS.gene63933:CDS
TGGAAGTCATAAAAGAATGA+AGG 0.477032 3.4:-10089208 None:intergenic
TGGACAAGAGATGCAAGATT+GGG 0.479430 3.4:+10090288 MS.gene63933:CDS
AGGCAGTCCTTCTTCTGGTA+AGG 0.487880 3.4:-10091490 None:intergenic
CAACGAGTTATGTCCTCAAA+TGG 0.488180 3.4:+10090377 MS.gene63933:CDS
ATTCATTGTTCCGTATTACA+TGG 0.489301 3.4:-10089918 None:intergenic
AATAAGGATTATCGCCCGTT+GGG 0.491675 3.4:+10089340 MS.gene63933:CDS
CAAATAACCATTGAAAAGAA+GGG 0.495588 3.4:-10089435 None:intergenic
AAAGGGTTGTCCATGTAATA+CGG 0.496762 3.4:+10089908 MS.gene63933:CDS
CGAGATCACTTGACAAAAGA+GGG 0.498904 3.4:+10088839 MS.gene63933:CDS
GTCCAACAAATACTCCCAAC+GGG 0.502839 3.4:-10089354 None:intergenic
GCCTAAATTGAAAATGAAAA+GGG 0.504520 3.4:+10089972 MS.gene63933:CDS
AAGATTCACGTGCTTTAAAG+AGG 0.507154 3.4:+10088796 MS.gene63933:CDS
TGTTGATTATACACCACACT+TGG 0.511127 3.4:+10088649 MS.gene63933:CDS
GCGTAAAGAATTTGAAAGAA+GGG 0.512208 3.4:+10090082 MS.gene63933:CDS
AATCATCTTCTTGAACCGCC+AGG 0.519049 3.4:+10088959 MS.gene63933:CDS
TTGTTATCAGTAATTATGCA+TGG 0.520487 3.4:-10091606 None:intergenic
GTTCGAAAACATTTCACTGC+AGG 0.524411 3.4:+10089790 MS.gene63933:CDS
TATAGTAGGAGAATTGGGTT+TGG 0.529308 3.4:+10088719 MS.gene63933:CDS
ACGAGATCACTTGACAAAAG+AGG 0.533209 3.4:+10088838 MS.gene63933:CDS
GGAGTTTGAATCCGAGGCAG+CGG 0.541901 3.4:+10088676 MS.gene63933:CDS
TCAACAATTCCAATAGTTGA+AGG 0.542316 3.4:+10088566 MS.gene63933:CDS
CTTGAATCCTTACCAGAAGA+AGG 0.545255 3.4:+10091483 MS.gene63933:CDS
TTTCCGGCTTGTACAAGCAT+AGG 0.546185 3.4:-10089997 None:intergenic
TCGAAAGATTGGTAAGTACA+AGG 0.548048 3.4:+10088913 MS.gene63933:CDS
AAATTCATATGCCGCTGCCT+CGG 0.550925 3.4:-10088687 None:intergenic
ATGCATTGATCTACATGAAG+AGG 0.551822 3.4:+10089657 MS.gene63933:CDS
AATGGTGGGATAGATGTTGT+TGG 0.552825 3.4:+10089100 MS.gene63933:CDS
AGGATGCGGTCACTAATGTA+TGG 0.554537 3.4:+10089139 MS.gene63933:CDS
TGCATGTTGATCAGTTAGAA+GGG 0.557107 3.4:-10089497 None:intergenic
GGAAGTAATCAAGATTTGGA+AGG 0.559886 3.4:+10090309 MS.gene63933:CDS
GTCACTAATGTATGGTGAAG+TGG 0.561332 3.4:+10089147 MS.gene63933:CDS
TTCTTCTTGAAATTCCTCAA+AGG 0.565966 3.4:-10090037 None:intergenic
CGCTTCAGTATATAGTGTTG+AGG 0.567827 3.4:-10090264 None:intergenic
TCGTTGTATCGCCTCATGAA+AGG 0.568522 3.4:-10090360 None:intergenic
AAATAAGGATTATCGCCCGT+TGG 0.568611 3.4:+10089339 MS.gene63933:CDS
GTCACTACTGAAAGAGAGGA+AGG 0.569317 3.4:-10091510 None:intergenic
GCTCCTATGCTTGTACAAGC+CGG 0.569380 3.4:+10089994 MS.gene63933:CDS
AACTGATCAACATGCAACAA+TGG 0.569412 3.4:+10089504 MS.gene63933:CDS
AACACTATATACTGAAGCGA+TGG 0.569686 3.4:+10090268 MS.gene63933:CDS
TCAAACTCCATTTCCAAGTG+TGG 0.572468 3.4:-10088662 None:intergenic
GGGAGAGTAGAAACCAGAAC+GGG 0.572783 3.4:+10088860 MS.gene63933:CDS
ACGATTATCTCAAGCTGTAG+CGG 0.579272 3.4:+10089024 MS.gene63933:CDS
CCAAGCTGTCAGAGCAAGGA+GGG 0.581065 3.4:+10091556 MS.gene63933:CDS
TGTCATGTATGTCGTATCAA+AGG 0.583944 3.4:-10089317 None:intergenic
GCCGACAATCTGAAATCTCA+AGG 0.586350 3.4:-10091458 None:intergenic
TCAACATGCAACAATGGCTA+AGG 0.587790 3.4:+10089510 MS.gene63933:CDS
TCACTAATGTATGGTGAAGT+GGG 0.590425 3.4:+10089148 MS.gene63933:CDS
ACTAAATAATCACAAGCAGA+TGG 0.591874 3.4:+10089375 MS.gene63933:CDS
GAAGTAATCAAGATTTGGAA+GGG 0.592932 3.4:+10090310 MS.gene63933:CDS
ATCATCTTCTTGAACCGCCA+GGG 0.601235 3.4:+10088960 MS.gene63933:CDS
GGAAATGGAGTTTGAATCCG+AGG 0.601453 3.4:+10088670 MS.gene63933:CDS
TGACAACAATGTCAACACTC+TGG 0.602258 3.4:+10090566 MS.gene63933:CDS
ATAAACGATTATGGATACTT+CGG 0.603159 3.4:+10089283 MS.gene63933:CDS
GATAGTCACTACTGAAAGAG+AGG 0.606713 3.4:-10091514 None:intergenic
TGATAATAATAAGGAGCGGA+CGG 0.606864 3.4:+10090125 MS.gene63933:CDS
ATCTTCAAACTAGAAGGATG+CGG 0.607370 3.4:+10089125 MS.gene63933:CDS
GTGACTCGGATATTTGTCGA+TGG 0.607546 3.4:-10089003 None:intergenic
TCTATCCATACCAATTGCCA+AGG 0.609778 3.4:+10090536 MS.gene63933:CDS
AGATTCACGTGCTTTAAAGA+GGG 0.609935 3.4:+10088797 MS.gene63933:CDS
ATGTGTACAAGCAAAATGGT+GGG 0.610110 3.4:+10089086 MS.gene63933:CDS
TGTCGTAAATTTGAGACACA+TGG 0.610330 3.4:+10090186 MS.gene63933:CDS
TCAACACTCTGGATGTTGGT+GGG 0.611788 3.4:+10090577 MS.gene63933:CDS
GGGGAGAGTAGAAACCAGAA+CGG 0.611921 3.4:+10088859 MS.gene63933:CDS
GATATTTGTCGATGGGAGCG+AGG 0.612184 3.4:-10088995 None:intergenic
AGCTTGAGATAATCGTGACT+CGG 0.618453 3.4:-10089017 None:intergenic
ACAACCAAGCTGTCAGAGCA+AGG 0.623605 3.4:+10091552 MS.gene63933:CDS
AGTCCAACAAATACTCCCAA+CGG 0.624240 3.4:-10089355 None:intergenic
CAACACTCTGGATGTTGGTG+GGG 0.627596 3.4:+10090578 MS.gene63933:CDS
TGACTCGGATATTTGTCGAT+GGG 0.631650 3.4:-10089002 None:intergenic
GTCAACACTCTGGATGTTGG+TGG 0.637024 3.4:+10090576 MS.gene63933:CDS
CAGAGCAAGGAGGGAACGAG+TGG 0.638796 3.4:+10091565 MS.gene63933:CDS
ATATAAAAGCTCCTTTCATG+AGG 0.642620 3.4:+10090349 MS.gene63933:CDS
TATGTGTACAAGCAAAATGG+TGG 0.642995 3.4:+10089085 MS.gene63933:CDS
TTGAGATTTCAGATTGTCGG+CGG 0.649583 3.4:+10091460 MS.gene63933:CDS
ATAAGGAGCGGACGGTAATG+GGG 0.649896 3.4:+10090133 MS.gene63933:CDS
AATGTCAACACTCTGGATGT+TGG 0.652365 3.4:+10090573 MS.gene63933:CDS
ATTGATCTACATGAAGAGGA+AGG 0.663372 3.4:+10089661 MS.gene63933:CDS
ATTGGTGAACAATATTGGTG+TGG 0.663839 3.4:+10088598 MS.gene63933:CDS
TGCTTTAAAGAGGGTAAACG+GGG 0.679692 3.4:+10088806 MS.gene63933:CDS
AATACGGAACAATGAATCAG+AGG 0.680081 3.4:+10089924 MS.gene63933:CDS
CGAGGAGTATGAAAATACCC+TGG 0.683837 3.4:-10088977 None:intergenic
ATTATGATAATAATAAGGAG+CGG 0.687685 3.4:+10090121 MS.gene63933:CDS
TGTGGATATTGTGCAAAATG+GGG 0.687726 3.4:+10088616 MS.gene63933:CDS
ACCAAGCTGTCAGAGCAAGG+AGG 0.707203 3.4:+10091555 MS.gene63933:CDS
GAGATCACTTGACAAAAGAG+GGG 0.708310 3.4:+10088840 MS.gene63933:CDS
GGAGTATGAAAATACCCTGG+CGG 0.746615 3.4:-10088974 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAATAATAAAGATGAAAAT+TGG - chr3.4:10091391-10091410 None:intergenic 10.0%
!! TACAAATTATGATAATAATA+AGG + chr3.4:10090116-10090135 MS.gene63933:CDS 10.0%
!!! AATTTTATAATAAGTATAGT+AGG + chr3.4:10088705-10088724 MS.gene63933:CDS 10.0%
!!! ATTATTTAAAATGCTTAAAA+GGG + chr3.4:10091403-10091422 MS.gene63933:intron 10.0%
!!! TATTATTTAAAATGCTTAAA+AGG + chr3.4:10091402-10091421 MS.gene63933:intron 10.0%
!! GAAAATTTACAATGAATTTA+AGG + chr3.4:10091190-10091209 MS.gene63933:intron 15.0%
!!! AGAAAATAACAAATGTATTT+TGG + chr3.4:10089245-10089264 MS.gene63933:CDS 15.0%
!!! GAAAATAACAAATGTATTTT+GGG + chr3.4:10089246-10089265 MS.gene63933:CDS 15.0%
!!! TAAAATAATTGATGATATGT+TGG + chr3.4:10090473-10090492 MS.gene63933:CDS 15.0%
!! ATTATGATAATAATAAGGAG+CGG + chr3.4:10090121-10090140 MS.gene63933:CDS 20.0%
!!! AAAATCTTTTTCTTTTTCAG+AGG + chr3.4:10091004-10091023 MS.gene63933:intron 20.0%
!!! TTTTCAATTGAAGGAAAAAT+GGG + chr3.4:10089756-10089775 MS.gene63933:CDS 20.0%
!!! TTTTTCAATTGAAGGAAAAA+TGG + chr3.4:10089755-10089774 MS.gene63933:CDS 20.0%
! AATAAGTATAGTAGGAGAAT+TGG + chr3.4:10088713-10088732 MS.gene63933:CDS 25.0%
! ATAAACGATTATGGATACTT+CGG + chr3.4:10089283-10089302 MS.gene63933:CDS 25.0%
! ATAAGTATAGTAGGAGAATT+GGG + chr3.4:10088714-10088733 MS.gene63933:CDS 25.0%
! ATGAATTCCTTGATGAAAAT+TGG + chr3.4:10091317-10091336 MS.gene63933:intron 25.0%
! ATGGATAAAGTTTGAAGAAA+GGG - chr3.4:10091047-10091066 None:intergenic 25.0%
! ATTAGACTCTTCAAAAACTT+TGG - chr3.4:10090454-10090473 None:intergenic 25.0%
! CAAATAACCATTGAAAAGAA+GGG - chr3.4:10089438-10089457 None:intergenic 25.0%
! CCTTCAATTGAAAAATCATA+CGG - chr3.4:10089750-10089769 None:intergenic 25.0%
! CTCAAATAATGCTAAAAAAC+GGG + chr3.4:10090627-10090646 MS.gene63933:intron 25.0%
! GCCTAAATTGAAAATGAAAA+GGG + chr3.4:10089972-10089991 MS.gene63933:CDS 25.0%
! GCTCAAATAATGCTAAAAAA+CGG + chr3.4:10090626-10090645 MS.gene63933:intron 25.0%
! GGAAATAAAAGCAAAAAAGA+TGG + chr3.4:10088758-10088777 MS.gene63933:CDS 25.0%
! TATGGATAAAGTTTGAAGAA+AGG - chr3.4:10091048-10091067 None:intergenic 25.0%
! TCAAATAACCATTGAAAAGA+AGG - chr3.4:10089439-10089458 None:intergenic 25.0%
! TCAAATAATGCTAAAAAACG+GGG + chr3.4:10090628-10090647 MS.gene63933:intron 25.0%
! TGCCTAAATTGAAAATGAAA+AGG + chr3.4:10089971-10089990 MS.gene63933:CDS 25.0%
! TTGATCAATTTAACCATTTG+AGG - chr3.4:10090393-10090412 None:intergenic 25.0%
! TTGTTATCAGTAATTATGCA+TGG - chr3.4:10091609-10091628 None:intergenic 25.0%
!! AATTTTTCACACACATTGAA+AGG + chr3.4:10089890-10089909 MS.gene63933:CDS 25.0%
!! ATAATGTTTCTGAAGATTCA+TGG + chr3.4:10089722-10089741 MS.gene63933:CDS 25.0%
!! ATTCTTTTATGACTTCCAAA+TGG + chr3.4:10089213-10089232 MS.gene63933:CDS 25.0%
!! ATTTTTCACACACATTGAAA+GGG + chr3.4:10089891-10089910 MS.gene63933:CDS 25.0%
ACTAAATAATCACAAGCAGA+TGG + chr3.4:10089375-10089394 MS.gene63933:CDS 30.0%
AGTAGACAAACTCTCAAAAT+AGG - chr3.4:10091229-10091248 None:intergenic 30.0%
ATATAAAAGCTCCTTTCATG+AGG + chr3.4:10090349-10090368 MS.gene63933:CDS 30.0%
ATTCATTGTTCCGTATTACA+TGG - chr3.4:10089921-10089940 None:intergenic 30.0%
ATTGGGAAGTAATCAAGATT+TGG + chr3.4:10090305-10090324 MS.gene63933:CDS 30.0%
ATTTCACTTGATCGAAAGAT+TGG + chr3.4:10088902-10088921 MS.gene63933:CDS 30.0%
CAATATGGTAAAGATTCATG+AGG - chr3.4:10090874-10090893 None:intergenic 30.0%
CATACACACTCAATTGATAA+AGG - chr3.4:10091141-10091160 None:intergenic 30.0%
CATTGTTGATGCATTTCAAA+CGG + chr3.4:10089173-10089192 MS.gene63933:CDS 30.0%
CTTTATCAATTGAGTGTGTA+TGG + chr3.4:10091139-10091158 MS.gene63933:intron 30.0%
GAAGAATATGAGGAATATCT+AGG + chr3.4:10090054-10090073 MS.gene63933:CDS 30.0%
GAAGTAATCAAGATTTGGAA+GGG + chr3.4:10090310-10090329 MS.gene63933:CDS 30.0%
GAATATGTGTACAAGCAAAA+TGG + chr3.4:10089082-10089101 MS.gene63933:CDS 30.0%
GAGAAATTGGTGAACAATAT+TGG + chr3.4:10088593-10088612 MS.gene63933:CDS 30.0%
GATTCTCACAAGAAAGAAAA+AGG - chr3.4:10090840-10090859 None:intergenic 30.0%
GCACAAATGATAAACGATTA+TGG + chr3.4:10089274-10089293 MS.gene63933:CDS 30.0%
GCGTAAAGAATTTGAAAGAA+GGG + chr3.4:10090082-10090101 MS.gene63933:CDS 30.0%
GGAATTTCAAGAAGAATATG+AGG + chr3.4:10090044-10090063 MS.gene63933:CDS 30.0%
TACGACATACATGACAAATA+AGG + chr3.4:10089324-10089343 MS.gene63933:CDS 30.0%
TCAACAATTCCAATAGTTGA+AGG + chr3.4:10088566-10088585 MS.gene63933:CDS 30.0%
TGCGTAAAGAATTTGAAAGA+AGG + chr3.4:10090081-10090100 MS.gene63933:CDS 30.0%
TGGAAGTCATAAAAGAATGA+AGG - chr3.4:10089211-10089230 None:intergenic 30.0%
TTCTTCTTGAAATTCCTCAA+AGG - chr3.4:10090040-10090059 None:intergenic 30.0%
TTGAACTCTCTTGATATAGA+TGG - chr3.4:10089615-10089634 None:intergenic 30.0%
TTGGTTATCTTCAAACTAGA+AGG + chr3.4:10089119-10089138 MS.gene63933:CDS 30.0%
! AAACAATCCCTTCTTTTCAA+TGG + chr3.4:10089428-10089447 MS.gene63933:CDS 30.0%
! GAAACTTTTCTCAAAGCAAT+GGG + chr3.4:10089457-10089476 MS.gene63933:CDS 30.0%
! GCCCTTTTCATTTTCAATTT+AGG - chr3.4:10089976-10089995 None:intergenic 30.0%
! GCTTTTATATCCAGTTCAAT+AGG - chr3.4:10090339-10090358 None:intergenic 30.0%
! TGAAACTTTTCTCAAAGCAA+TGG + chr3.4:10089456-10089475 MS.gene63933:CDS 30.0%
! TGAAATTCCTCAAAGGTTTT+TGG - chr3.4:10090033-10090052 None:intergenic 30.0%
! TTCAAAAGTCTTTTCATCTC+AGG + chr3.4:10090969-10090988 MS.gene63933:intron 30.0%
! TTTTCTCTTCAACATCCATT+TGG - chr3.4:10089231-10089250 None:intergenic 30.0%
!! ATGAAAAGACTTTTGAAGAC+TGG - chr3.4:10090966-10090985 None:intergenic 30.0%
!! CCGTATGATTTTTCAATTGA+AGG + chr3.4:10089747-10089766 MS.gene63933:CDS 30.0%
!! TGAAAAGACTTTTGAAGACT+GGG - chr3.4:10090965-10090984 None:intergenic 30.0%
!!! TTTTGGAGTGTAAATGTTTC+CGG - chr3.4:10090016-10090035 None:intergenic 30.0%
AAAGGGTTGTCCATGTAATA+CGG + chr3.4:10089908-10089927 MS.gene63933:CDS 35.0%
AACACTATATACTGAAGCGA+TGG + chr3.4:10090268-10090287 MS.gene63933:CDS 35.0%
AACTGATCAACATGCAACAA+TGG + chr3.4:10089504-10089523 MS.gene63933:CDS 35.0%
AAGATTGGGACAAGCATATA+TGG - chr3.4:10091066-10091085 None:intergenic 35.0%
AAGGAGAGATTGTAATGTTG+AGG - chr3.4:10090821-10090840 None:intergenic 35.0%
AATACGGAACAATGAATCAG+AGG + chr3.4:10089924-10089943 MS.gene63933:CDS 35.0%
AATTGAAGGAAAAATGGGCA+TGG + chr3.4:10089761-10089780 MS.gene63933:CDS 35.0%
ACCTTGAGATTTCAGATTGT+CGG + chr3.4:10091457-10091476 MS.gene63933:CDS 35.0%
AGGAGAGATTGTAATGTTGA+GGG - chr3.4:10090820-10090839 None:intergenic 35.0%
ATAATGCTAAAAAACGGGGT+TGG + chr3.4:10090632-10090651 MS.gene63933:intron 35.0%
ATCTTCAAACTAGAAGGATG+CGG + chr3.4:10089125-10089144 MS.gene63933:CDS 35.0%
ATGTGTACAAGCAAAATGGT+GGG + chr3.4:10089086-10089105 MS.gene63933:CDS 35.0%
ATTCACAACTCTGTAAGACA+AGG - chr3.4:10091281-10091300 None:intergenic 35.0%
ATTGAAGGAAAAATGGGCAT+GGG + chr3.4:10089762-10089781 MS.gene63933:CDS 35.0%
ATTGATCTACATGAAGAGGA+AGG + chr3.4:10089661-10089680 MS.gene63933:CDS 35.0%
ATTGGTGAACAATATTGGTG+TGG + chr3.4:10088598-10088617 MS.gene63933:CDS 35.0%
CACAATCCATGAAATGTTTC+CGG - chr3.4:10089553-10089572 None:intergenic 35.0%
CTCATGTCTTGACTCATAAT+CGG - chr3.4:10089950-10089969 None:intergenic 35.0%
GGAAGTAATCAAGATTTGGA+AGG + chr3.4:10090309-10090328 MS.gene63933:CDS 35.0%
GTGTGGATATTGTGCAAAAT+GGG + chr3.4:10088615-10088634 MS.gene63933:CDS 35.0%
TATAGTAGGAGAATTGGGTT+TGG + chr3.4:10088719-10088738 MS.gene63933:CDS 35.0%
TATATGCTTGTCCCAATCTT+CGG + chr3.4:10091066-10091085 MS.gene63933:intron 35.0%
TATGTGTACAAGCAAAATGG+TGG + chr3.4:10089085-10089104 MS.gene63933:CDS 35.0%
TCACTAATGTATGGTGAAGT+GGG + chr3.4:10089148-10089167 MS.gene63933:CDS 35.0%
TCGAAAGATTGGTAAGTACA+AGG + chr3.4:10088913-10088932 MS.gene63933:CDS 35.0%
TGATAATAATAAGGAGCGGA+CGG + chr3.4:10090125-10090144 MS.gene63933:CDS 35.0%
TGCATGTTGATCAGTTAGAA+GGG - chr3.4:10089500-10089519 None:intergenic 35.0%
TGTCATGTATGTCGTATCAA+AGG - chr3.4:10089320-10089339 None:intergenic 35.0%
TGTGGATATTGTGCAAAATG+GGG + chr3.4:10088616-10088635 MS.gene63933:CDS 35.0%
TTACACTCCAAAAACCTTTG+AGG + chr3.4:10090023-10090042 MS.gene63933:CDS 35.0%
TTATACACCACACTTGGAAA+TGG + chr3.4:10088655-10088674 MS.gene63933:CDS 35.0%
TTCACAACTCTGTAAGACAA+GGG - chr3.4:10091280-10091299 None:intergenic 35.0%
TTCTCAAGTGAAGTGCAATA+TGG - chr3.4:10090889-10090908 None:intergenic 35.0%
TTCTCTACACCTTCAACTAT+TGG - chr3.4:10088578-10088597 None:intergenic 35.0%
TTGCATGTTGATCAGTTAGA+AGG - chr3.4:10089501-10089520 None:intergenic 35.0%
TTTGGTATTCGTCGAGAATA+TGG + chr3.4:10088737-10088756 MS.gene63933:CDS 35.0%
! AGTTGAAGGTGTAGAGAAAT+TGG + chr3.4:10088580-10088599 MS.gene63933:CDS 35.0%
! ATGCATTGATCTACATGAAG+AGG + chr3.4:10089657-10089676 MS.gene63933:CDS 35.0%
! ATGTTGGCTAAAAAGTTCGT+GGG + chr3.4:10090489-10090508 MS.gene63933:CDS 35.0%
! ATTGTTGTCACGTTTTTCCT+TGG - chr3.4:10090556-10090575 None:intergenic 35.0%
! TATGTTGGCTAAAAAGTTCG+TGG + chr3.4:10090488-10090507 MS.gene63933:CDS 35.0%
! TGACTCTCCAATTTTCATCA+AGG - chr3.4:10091327-10091346 None:intergenic 35.0%
! TGATCAGTTAGAAGGGTTTT+AGG - chr3.4:10089493-10089512 None:intergenic 35.0%
! TGTCGTAAATTTGAGACACA+TGG + chr3.4:10090186-10090205 MS.gene63933:CDS 35.0%
! TGTTGATTATACACCACACT+TGG + chr3.4:10088649-10088668 MS.gene63933:CDS 35.0%
! TTTTTCCTTGGCAATTGGTA+TGG - chr3.4:10090544-10090563 None:intergenic 35.0%
!! AAGATTCACGTGCTTTAAAG+AGG + chr3.4:10088796-10088815 MS.gene63933:CDS 35.0%
!! AGATTCACGTGCTTTAAAGA+GGG + chr3.4:10088797-10088816 MS.gene63933:CDS 35.0%
!! GGCTATTTCTTTAGTTATGC+CGG + chr3.4:10089531-10089550 MS.gene63933:CDS 35.0%
AAATAAGGATTATCGCCCGT+TGG + chr3.4:10089339-10089358 MS.gene63933:CDS 40.0%
AAGTGGAAATGACCGAAGAT+TGG - chr3.4:10091081-10091100 None:intergenic 40.0%
AATAAGGATTATCGCCCGTT+GGG + chr3.4:10089340-10089359 MS.gene63933:CDS 40.0%
AATGTCAACACTCTGGATGT+TGG + chr3.4:10090573-10090592 MS.gene63933:CDS 40.0%
ACGAGATCACTTGACAAAAG+AGG + chr3.4:10088838-10088857 MS.gene63933:CDS 40.0%
ACGATTATCTCAAGCTGTAG+CGG + chr3.4:10089024-10089043 MS.gene63933:CDS 40.0%
AGCTTGAGATAATCGTGACT+CGG - chr3.4:10089020-10089039 None:intergenic 40.0%
AGTCCAACAAATACTCCCAA+CGG - chr3.4:10089358-10089377 None:intergenic 40.0%
AGTGGAAATGACCGAAGATT+GGG - chr3.4:10091080-10091099 None:intergenic 40.0%
ATGGACAAGAGATGCAAGAT+TGG + chr3.4:10090287-10090306 MS.gene63933:CDS 40.0%
CAACGAGTTATGTCCTCAAA+TGG + chr3.4:10090377-10090396 MS.gene63933:CDS 40.0%
CGAGATCACTTGACAAAAGA+GGG + chr3.4:10088839-10088858 MS.gene63933:CDS 40.0%
CGCTTCAGTATATAGTGTTG+AGG - chr3.4:10090267-10090286 None:intergenic 40.0%
CTTGAATCCTTACCAGAAGA+AGG + chr3.4:10091483-10091502 MS.gene63933:CDS 40.0%
GAGATCACTTGACAAAAGAG+GGG + chr3.4:10088840-10088859 MS.gene63933:CDS 40.0%
GATAGTCACTACTGAAAGAG+AGG - chr3.4:10091517-10091536 None:intergenic 40.0%
GGAGAGATTGTAATGTTGAG+GGG - chr3.4:10090819-10090838 None:intergenic 40.0%
GGGGAATCTTATGGATCAAA+AGG + chr3.4:10090152-10090171 MS.gene63933:CDS 40.0%
GGTGTGGATATTGTGCAAAA+TGG + chr3.4:10088614-10088633 MS.gene63933:CDS 40.0%
GTCACTAATGTATGGTGAAG+TGG + chr3.4:10089147-10089166 MS.gene63933:CDS 40.0%
GTGATTCATTGACATCCTCT+CGG + chr3.4:10090929-10090948 MS.gene63933:intron 40.0%
GTTATGCCGGAAACATTTCA+TGG + chr3.4:10089544-10089563 MS.gene63933:CDS 40.0%
GTTCGAAAACATTTCACTGC+AGG + chr3.4:10089790-10089809 MS.gene63933:CDS 40.0%
TCAAACTCCATTTCCAAGTG+TGG - chr3.4:10088665-10088684 None:intergenic 40.0%
TCAACATGCAACAATGGCTA+AGG + chr3.4:10089510-10089529 MS.gene63933:CDS 40.0%
TCCGACTATATGAGAGAAGT+CGG - chr3.4:10091356-10091375 None:intergenic 40.0%
TCCGACTTCTCTCATATAGT+CGG + chr3.4:10091352-10091371 MS.gene63933:intron 40.0%
TCTATCCATACCAATTGCCA+AGG + chr3.4:10090536-10090555 MS.gene63933:CDS 40.0%
TGACAACAATGTCAACACTC+TGG + chr3.4:10090566-10090585 MS.gene63933:CDS 40.0%
TGAGCATTCAGTCTATGAAG+TGG - chr3.4:10091098-10091117 None:intergenic 40.0%
TGGACAAGAGATGCAAGATT+GGG + chr3.4:10090288-10090307 MS.gene63933:CDS 40.0%
TGTCTACTTCAGCTATCACT+AGG + chr3.4:10091241-10091260 MS.gene63933:intron 40.0%
TTGAGATTTCAGATTGTCGG+CGG + chr3.4:10091460-10091479 MS.gene63933:CDS 40.0%
TTGAGGGGAGAACATTTCTT+GGG - chr3.4:10090804-10090823 None:intergenic 40.0%
! AATGGTGGGATAGATGTTGT+TGG + chr3.4:10089100-10089119 MS.gene63933:CDS 40.0%
! ACTTTTCTCAAAGCAATGGG+TGG + chr3.4:10089460-10089479 MS.gene63933:CDS 40.0%
! CATTTCATGGATTGTGCACT+TGG + chr3.4:10089557-10089576 MS.gene63933:CDS 40.0%
! TCACGTTTTTCCTTGGCAAT+TGG - chr3.4:10090549-10090568 None:intergenic 40.0%
! TGACTCGGATATTTGTCGAT+GGG - chr3.4:10089005-10089024 None:intergenic 40.0%
! TGTTGGCTAAAAAGTTCGTG+GGG + chr3.4:10090490-10090509 MS.gene63933:CDS 40.0%
!! AAGGTGTGTTTTTGAACGCA+TGG + chr3.4:10089680-10089699 MS.gene63933:CDS 40.0%
!! CACAAGCAGATGGTTGTTTT+TGG + chr3.4:10089385-10089404 MS.gene63933:CDS 40.0%
!! CGTGCTTTAAAGAGGGTAAA+CGG + chr3.4:10088804-10088823 MS.gene63933:CDS 40.0%
!! GTGCTTTAAAGAGGGTAAAC+GGG + chr3.4:10088805-10088824 MS.gene63933:CDS 40.0%
!! TGCTTTAAAGAGGGTAAACG+GGG + chr3.4:10088806-10088825 MS.gene63933:CDS 40.0%
AAAGACCATCACATCCTGAG+TGG + chr3.4:10090726-10090745 MS.gene63933:intron 45.0%
AAATTCATATGCCGCTGCCT+CGG - chr3.4:10088690-10088709 None:intergenic 45.0%
AATAAGGAGCGGACGGTAAT+GGG + chr3.4:10090132-10090151 MS.gene63933:CDS 45.0%
AATCATCTTCTTGAACCGCC+AGG + chr3.4:10088959-10088978 MS.gene63933:CDS 45.0%
AGGATGCGGTCACTAATGTA+TGG + chr3.4:10089139-10089158 MS.gene63933:CDS 45.0%
AGGCTAGACAGACTCTGAAT+TGG - chr3.4:10091121-10091140 None:intergenic 45.0%
AGTCTAATCACCATCCACTC+AGG - chr3.4:10090743-10090762 None:intergenic 45.0%
ATCATCTTCTTGAACCGCCA+GGG + chr3.4:10088960-10088979 MS.gene63933:CDS 45.0%
CTACTTCAGCTATCACTAGG+CGG + chr3.4:10091244-10091263 MS.gene63933:intron 45.0%
GAGTTGTGAATTCGAGACGA+TGG + chr3.4:10091290-10091309 MS.gene63933:intron 45.0%
GGAAATGGAGTTTGAATCCG+AGG + chr3.4:10088670-10088689 MS.gene63933:CDS 45.0%
GGAGTATGAAAATACCCTGG+CGG - chr3.4:10088977-10088996 None:intergenic 45.0%
GTCACTACTGAAAGAGAGGA+AGG - chr3.4:10091513-10091532 None:intergenic 45.0%
GTCCAACAAATACTCCCAAC+GGG - chr3.4:10089357-10089376 None:intergenic 45.0%
GTTGAGGGGAGAACATTTCT+TGG - chr3.4:10090805-10090824 None:intergenic 45.0%
GTTGGCTAAAAAGTTCGTGG+GGG + chr3.4:10090491-10090510 MS.gene63933:CDS 45.0%
TAATAAGGAGCGGACGGTAA+TGG + chr3.4:10090131-10090150 MS.gene63933:CDS 45.0%
TACTTCAGCTATCACTAGGC+GGG + chr3.4:10091245-10091264 MS.gene63933:intron 45.0%
TCAACACTCTGGATGTTGGT+GGG + chr3.4:10090577-10090596 MS.gene63933:CDS 45.0%
TCGTTGTATCGCCTCATGAA+AGG - chr3.4:10090363-10090382 None:intergenic 45.0%
! CGAGGAGTATGAAAATACCC+TGG - chr3.4:10088980-10088999 None:intergenic 45.0%
! GCCGACAATCTGAAATCTCA+AGG - chr3.4:10091461-10091480 None:intergenic 45.0%
! GTGACTCGGATATTTGTCGA+TGG - chr3.4:10089006-10089025 None:intergenic 45.0%
! TTTCCGGCTTGTACAAGCAT+AGG - chr3.4:10090000-10090019 None:intergenic 45.0%
!! GACGGTAATGGGGAATCTTA+TGG + chr3.4:10090143-10090162 MS.gene63933:CDS 45.0%
ACAACCAAGCTGTCAGAGCA+AGG + chr3.4:10091552-10091571 MS.gene63933:CDS 50.0%
ACCATCACATCCTGAGTGGA+TGG + chr3.4:10090730-10090749 MS.gene63933:intron 50.0%
ACCATCCACTCAGGATGTGA+TGG - chr3.4:10090734-10090753 None:intergenic 50.0%
ACTTCAGCTATCACTAGGCG+GGG + chr3.4:10091246-10091265 MS.gene63933:intron 50.0%
AGGCAGTCCTTCTTCTGGTA+AGG - chr3.4:10091493-10091512 None:intergenic 50.0%
ATAAGGAGCGGACGGTAATG+GGG + chr3.4:10090133-10090152 MS.gene63933:CDS 50.0%
CAACACTCTGGATGTTGGTG+GGG + chr3.4:10090578-10090597 MS.gene63933:CDS 50.0%
GATATTTGTCGATGGGAGCG+AGG - chr3.4:10088998-10089017 None:intergenic 50.0%
GCTCCTATGCTTGTACAAGC+CGG + chr3.4:10089994-10090013 MS.gene63933:CDS 50.0%
GGAAGGGCAACCTATTGAAC+TGG + chr3.4:10090326-10090345 MS.gene63933:CDS 50.0%
GGGAGAGTAGAAACCAGAAC+GGG + chr3.4:10088860-10088879 MS.gene63933:CDS 50.0%
GGGGAGAACATTTCTTGGGT+AGG - chr3.4:10090800-10090819 None:intergenic 50.0%
GGGGAGAGTAGAAACCAGAA+CGG + chr3.4:10088859-10088878 MS.gene63933:CDS 50.0%
GTAAATGACACTGCGACGAC+TGG - chr3.4:10090665-10090684 None:intergenic 50.0%
GTATGGACGTCGAAAACTGC+AGG + chr3.4:10091156-10091175 MS.gene63933:intron 50.0%
GTCAACACTCTGGATGTTGG+TGG + chr3.4:10090576-10090595 MS.gene63933:CDS 50.0%
! CAGGTCACAGCATCGAAAAG+AGG + chr3.4:10090686-10090705 MS.gene63933:intron 50.0%
!! CGTCGCAGTGTCATTTACTC+AGG + chr3.4:10090667-10090686 MS.gene63933:intron 50.0%
AACGGGATGTGATGCGCGTA+TGG + chr3.4:10088877-10088896 MS.gene63933:CDS 55.0%
ACCAAGCTGTCAGAGCAAGG+AGG + chr3.4:10091555-10091574 MS.gene63933:CDS 55.0%
CCAAGCTGTCAGAGCAAGGA+GGG + chr3.4:10091556-10091575 MS.gene63933:CDS 55.0%
GAGGAAGGCAGTCCTTCTTC+TGG - chr3.4:10091498-10091517 None:intergenic 55.0%
GGAGTTTGAATCCGAGGCAG+CGG + chr3.4:10088676-10088695 MS.gene63933:CDS 55.0%
!! CGCCCGTTGGGAGTATTTGT+TGG + chr3.4:10089352-10089371 MS.gene63933:CDS 55.0%
ACGCGCATCACATCCCGTTC+TGG - chr3.4:10088876-10088895 None:intergenic 60.0%
CAGAGCAAGGAGGGAACGAG+TGG + chr3.4:10091565-10091584 MS.gene63933:CDS 60.0%
CCCTCCTTGCTCTGACAGCT+TGG - chr3.4:10091559-10091578 None:intergenic 60.0%
CTGGGAGAGAACTAGCCGAG+AGG - chr3.4:10090947-10090966 None:intergenic 60.0%
GCTGTAGCGGCTGACGAATC+TGG + chr3.4:10089037-10089056 MS.gene63933:CDS 60.0%
! CTGGATGTTGGTGGGGAGCA+GGG + chr3.4:10090585-10090604 MS.gene63933:CDS 60.0%
! TCTGGATGTTGGTGGGGAGC+AGG + chr3.4:10090584-10090603 MS.gene63933:CDS 60.0%


Chromosome Type Strat End Strand Name
chr3.4 gene 10088557 10091641 10088557 ID=MS.gene63933
chr3.4 mRNA 10088557 10091641 10088557 ID=MS.gene63933.t1;Parent=MS.gene63933
chr3.4 exon 10088557 10090606 10088557 ID=MS.gene63933.t1.exon1;Parent=MS.gene63933.t1
chr3.4 CDS 10088557 10090606 10088557 ID=cds.MS.gene63933.t1;Parent=MS.gene63933.t1
chr3.4 exon 10091424 10091641 10091424 ID=MS.gene63933.t1.exon2;Parent=MS.gene63933.t1
chr3.4 CDS 10091424 10091641 10091424 ID=cds.MS.gene63933.t1;Parent=MS.gene63933.t1
Gene Sequence

>MS.gene63933

ATGAATGGGTCAACAATTCCAATAGTTGAAGGTGTAGAGAAATTGGTGAACAATATTGGTGTGGATATTGTGCAAAATGGGGAAGAGAGTGTTGTTGATTATACACCACACTTGGAAATGGAGTTTGAATCCGAGGCAGCGGCATATGAATTTTATAATAAGTATAGTAGGAGAATTGGGTTTGGTATTCGTCGAGAATATGGAAATAAAAGCAAAAAAGATGGAATTCTAACTTCAAGAAGATTCACGTGCTTTAAAGAGGGTAAACGGGGTGTTGACAAACGAGATCACTTGACAAAAGAGGGGAGAGTAGAAACCAGAACGGGATGTGATGCGCGTATGGTTATTTCACTTGATCGAAAGATTGGTAAGTACAAGGTTGTTGACTTTGTAGCTCAACATAATCATCTTCTTGAACCGCCAGGGTATTTTCATACTCCTCGCTCCCATCGACAAATATCCGAGTCACGATTATCTCAAGCTGTAGCGGCTGACGAATCTGGATTAAAACGAAAAGATTTTCATGAATATGTGTACAAGCAAAATGGTGGGATAGATGTTGTTGGTTATCTTCAAACTAGAAGGATGCGGTCACTAATGTATGGTGAAGTGGGTGCATTGTTGATGCATTTCAAACGGCAAAGTGAAAATCCTTCATTCTTTTATGACTTCCAAATGGATGTTGAAGAGAAAATAACAAATGTATTTTGGGCAGATGCACAAATGATAAACGATTATGGATACTTCGGTGATGTCATCACCTTTGATACGACATACATGACAAATAAGGATTATCGCCCGTTGGGAGTATTTGTTGGACTAAATAATCACAAGCAGATGGTTGTTTTTGGTGCAACATTGCTATATGATGAAACAATCCCTTCTTTTCAATGGTTATTTGAAACTTTTCTCAAAGCAATGGGTGGAGAAAAGCCTAAAACCCTTCTAACTGATCAACATGCAACAATGGCTAAGGCTATTTCTTTAGTTATGCCGGAAACATTTCATGGATTGTGCACTTGGCGTATGAGAGAAAATGCACAGAGACATGTGAACCATCTATATCAAGAGAGTTCAAAATTCTGCTCAGATTTTGAAGCATGCATTGATCTACATGAAGAGGAAGGTGTGTTTTTGAACGCATGGAATGTTTTACTTGTCGAACATAATGTTTCTGAAGATTCATGGCTCCGTATGATTTTTCAATTGAAGGAAAAATGGGCATGGGCGTATGTTCGAAAACATTTCACTGCAGGTATGAGATCTACTCAAATAAGTGAGAGTTTTAATGCTGAGTTGAAAAATTATTTGAAGTCAGATTTAAATTTAGTTCAATTTTTCACACACATTGAAAGGGTTGTCCATGTAATACGGAACAATGAATCAGAGGCCGATTATGAGTCAAGACATGAGTTGCCTAAATTGAAAATGAAAAGGGCTCCTATGCTTGTACAAGCCGGAAACATTTACACTCCAAAAACCTTTGAGGAATTTCAAGAAGAATATGAGGAATATCTAGGCACTTGCGTAAAGAATTTGAAAGAAGGGTTGTATGCTGTTACAAATTATGATAATAATAAGGAGCGGACGGTAATGGGGAATCTTATGGATCAAAAGGTTGTCTGTGATTGTCGTAAATTTGAGACACATGGCATCTTATGCAGTCATGCTTTGAAAGTATTAGATGTTATGAATATTAAGCTGATTCCTCAACACTATATACTGAAGCGATGGACAAGAGATGCAAGATTGGGAAGTAATCAAGATTTGGAAGGGCAACCTATTGAACTGGATATAAAAGCTCCTTTCATGAGGCGATACAACGAGTTATGTCCTCAAATGGTTAAATTGATCAACAAAGCATCAAAATCTCATGAAACTTACACATTTTTATCCAAAGTTTTTGAAGAGTCTAATAAAATAATTGATGATATGTTGGCTAAAAAGTTCGTGGGGGCAGAATCACTTGAGCTGTGTCATCTATCCATACCAATTGCCAAGGAAAAACGTGACAACAATGTCAACACTCTGGATGTTGGTGGGGAGCAGGGTATAAAGAAAGCAAGATTGCTCAAATAATGCTAAAAAACGGGGTTGGACATAATCCAGTCGTCGCAGTGTCATTTACTCAGGTCACAGCATCGAAAAGAGGTGAATAGCAGCAGCATAAAAGACCATCACATCCTGAGTGGATGGTGATTAGACTTAATTCTATTAAAGAGTTCAAGTTTCTCTTAGATCCTACCCAAGAAATGTTCTCCCCTCAACATTACAATCTCTCCTTTTTCTTTCTTGTGAGAATCTAGAATTCTTACCTCATGAATCTTTACCATATTGCACTTCACTTGAGAAATTGAAAATATTCAATATCTGTGATTCATTGACATCCTCTCGGCTAGTTCTCTCCCAGTCTTCAAAAGTCTTTTCATCTCAGGTTGTAAAAATCTAAAATCTTTTTCTTTTTCAGAGGAAGCGTTGCATAGTCATCCCTTTCTTCAAACTTTATCCATATATGCTTGTCCCAATCTTCGGTCATTTCCACTTCATAGACTGAATGCTCACCAATTCAGAGTCTGTCTAGCCTTTATCAATTGAGTGTGTATGGACGTCGAAAACTGCAGGCTTTTGCTCAAGAAAATTTACAATGAATTTAAGGATACTTGAAGTGTCCTATTTTGAGAGTTTGTCTACTTCAGCTATCACTAGGCGGGGTTTGCATTCCCTTGTCTTACAGAGTTGTGAATTCGAGACGATGGTCGTATGAATTCCTTGATGAAAATTGGAGAGTCATTGCTTCCGACTTCTCTCATATAGTCGGAAGCAATGACTCTCCAATTTTCATCTTTATTATTTAAAATGCTTAAAAGGGAAGTTGCTTCAGCATCTCACATCTCTTGAAAACCTTGAGATTTCAGATTGTCGGCGGCTTGAATCCTTACCAGAAGAAGGACTGCCTTCCTCTCTTTCAGTAGTGACTATCAATGATGCTTTTACTACAACCAAGCTGTCAGAGCAAGGAGGGAACGAGTGGCATAAGATTGCACATAATCCATGCATAATTACTGATAACAAAGTGATAATCTGA
Protein sequence

>MS.gene63933.t1

MNGSTIPIVEGVEKLVNNIGVDIVQNGEESVVDYTPHLEMEFESEAAAYEFYNKYSRRIGFGIRREYGNKSKKDGILTSRRFTCFKEGKRGVDKRDHLTKEGRVETRTGCDARMVISLDRKIGKYKVVDFVAQHNHLLEPPGYFHTPRSHRQISESRLSQAVAADESGLKRKDFHEYVYKQNGGIDVVGYLQTRRMRSLMYGEVGALLMHFKRQSENPSFFYDFQMDVEEKITNVFWADAQMINDYGYFGDVITFDTTYMTNKDYRPLGVFVGLNNHKQMVVFGATLLYDETIPSFQWLFETFLKAMGGEKPKTLLTDQHATMAKAISLVMPETFHGLCTWRMRENAQRHVNHLYQESSKFCSDFEACIDLHEEEGVFLNAWNVLLVEHNVSEDSWLRMIFQLKEKWAWAYVRKHFTAGMRSTQISESFNAELKNYLKSDLNLVQFFTHIERVVHVIRNNESEADYESRHELPKLKMKRAPMLVQAGNIYTPKTFEEFQEEYEEYLGTCVKNLKEGLYAVTNYDNNKERTVMGNLMDQKVVCDCRKFETHGILCSHALKVLDVMNIKLIPQHYILKRWTRDARLGSNQDLEGQPIELDIKAPFMRRYNELCPQMVKLINKASKSHETYTFLSKVFEESNKIIDDMLAKKFVGAESLELCHLSIPIAKEKRDNNVNTLDVGGEQGKLLQHLTSLENLEISDCRRLESLPEEGLPSSLSVVTINDAFTTTKLSEQGGNEWHKIAHNPCIITDNKVII