Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64514.t1 | XP_003625515.1 | 100 | 143 | 0 | 0 | 1 | 143 | 1 | 143 | 4.90E-74 | 287 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64514.t1 | O82201 | 91.5 | 141 | 12 | 0 | 1 | 141 | 1 | 141 | 2.2e-70 | 266.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64514.t1 | G7L0L8 | 100.0 | 143 | 0 | 0 | 1 | 143 | 1 | 143 | 3.6e-74 | 287.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene64514 | MS.gene27073 | PPI |
| MS.gene64514 | MS.gene017498 | PPI |
| MS.gene64514 | MS.gene64216 | PPI |
| MS.gene64514 | MS.gene021005 | PPI |
| MS.gene64514 | MS.gene75781 | PPI |
| MS.gene64514 | MS.gene64013 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64514.t1 | MTR_7g100010 | 100.000 | 143 | 0 | 0 | 1 | 143 | 1 | 143 | 2.71e-104 | 294 |
| MS.gene64514.t1 | MTR_8g102180 | 100.000 | 143 | 0 | 0 | 1 | 143 | 1 | 143 | 2.71e-104 | 294 |
| MS.gene64514.t1 | MTR_5g098940 | 42.754 | 138 | 79 | 0 | 3 | 140 | 2 | 139 | 6.08e-39 | 129 |
| MS.gene64514.t1 | MTR_1g025190 | 46.763 | 139 | 74 | 0 | 3 | 141 | 2 | 140 | 2.03e-38 | 127 |
| MS.gene64514.t1 | MTR_5g087820 | 42.537 | 134 | 77 | 0 | 3 | 136 | 26 | 159 | 4.96e-35 | 120 |
| MS.gene64514.t1 | MTR_8g077600 | 37.838 | 148 | 79 | 3 | 3 | 141 | 2 | 145 | 7.50e-31 | 108 |
| MS.gene64514.t1 | MTR_8g077600 | 36.559 | 93 | 46 | 3 | 3 | 86 | 2 | 90 | 8.93e-12 | 58.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64514.t1 | AT2G19790 | 90.845 | 142 | 13 | 0 | 1 | 142 | 1 | 142 | 1.27e-96 | 275 |
| MS.gene64514.t1 | AT1G47830 | 47.482 | 139 | 73 | 0 | 3 | 141 | 2 | 140 | 3.80e-39 | 129 |
| MS.gene64514.t1 | AT2G17380 | 42.446 | 139 | 80 | 0 | 3 | 141 | 2 | 140 | 9.73e-39 | 129 |
| MS.gene64514.t1 | AT4G35410 | 42.446 | 139 | 80 | 0 | 3 | 141 | 2 | 140 | 1.94e-37 | 125 |
| MS.gene64514.t1 | AT3G50860 | 40.278 | 144 | 81 | 1 | 3 | 141 | 2 | 145 | 3.65e-35 | 120 |
| MS.gene64514.t1 | AT3G50860 | 40.278 | 144 | 81 | 1 | 3 | 141 | 2 | 145 | 3.72e-35 | 119 |
| MS.gene64514.t1 | AT4G35410 | 46.392 | 97 | 52 | 0 | 3 | 99 | 2 | 98 | 1.37e-25 | 94.0 |
Find 21 sgRNAs with CRISPR-Local
Find 98 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCAGAATGAGCTTGCTATTT+TGG | 0.125124 | 7.3:-12612762 | MS.gene64514:intron |
| GAAACCATGGATCGACATTT+TGG | 0.210891 | 7.3:-12612719 | MS.gene64514:CDS |
| TCTGACTCCTATTCAGCTTA+TGG | 0.236679 | 7.3:-12612412 | MS.gene64514:CDS |
| GGGGATCCGATTCGTGTTAA+TGG | 0.262612 | 7.3:-12614158 | MS.gene64514:CDS |
| TACGAACGATTTCGCCTTCA+AGG | 0.388788 | 7.3:+12614068 | None:intergenic |
| TGTTCACCATTAACACGAAT+CGG | 0.437301 | 7.3:+12614152 | None:intergenic |
| TATCATGTTTCATTTAGAAA+AGG | 0.473226 | 7.3:-12612499 | MS.gene64514:CDS |
| GAAGAACGACGGACCCTTGA+AGG | 0.487753 | 7.3:-12614082 | MS.gene64514:CDS |
| GCAACTACAAAATTGTATAC+AGG | 0.518035 | 7.3:-12613159 | MS.gene64514:CDS |
| ACGAACGATTTCGCCTTCAA+GGG | 0.529007 | 7.3:+12614069 | None:intergenic |
| TTTCTGGTTGGAGTTGATGA+CGG | 0.545713 | 7.3:-12613118 | MS.gene64514:CDS |
| GGTCATGAATGGTTGTATTG+TGG | 0.573456 | 7.3:-12612454 | MS.gene64514:CDS |
| CCTCGCCCGTAATGAAAACC+AGG | 0.578527 | 7.3:-12614041 | MS.gene64514:intron |
| CCATGGTTTCGACTAAGAGA+TGG | 0.580792 | 7.3:+12612732 | None:intergenic |
| GTTGCCAAAATGTCGATCCA+TGG | 0.581731 | 7.3:+12612715 | None:intergenic |
| GTGTTAATGGTGAACAAACA+AGG | 0.585214 | 7.3:-12614145 | MS.gene64514:CDS |
| TTGGAGGAAATGGTCATGAA+TGG | 0.600198 | 7.3:-12612465 | MS.gene64514:CDS |
| CCATCTCTTAGTCGAAACCA+TGG | 0.604583 | 7.3:-12612732 | MS.gene64514:CDS |
| ACCTCACTGTTGAAGAACGA+CGG | 0.647147 | 7.3:-12614093 | MS.gene64514:CDS |
| GGTTGGAGTTGATGACGGCG+AGG | 0.687099 | 7.3:-12613113 | MS.gene64514:intron |
| TCCGTCGTTCTTCAACAGTG+AGG | 0.722658 | 7.3:+12614092 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CTTAATAAAATAAAAAACTT+AGG | - | chr7.3:12613133-12613152 | MS.gene64514:CDS | 10.0% |
| !! | ATAAATAATAAACAATGACA+AGG | + | chr7.3:12612611-12612630 | None:intergenic | 15.0% |
| !! | ATGAAAATAAAAGAAAAGAA+TGG | + | chr7.3:12612550-12612569 | None:intergenic | 15.0% |
| !!! | TTTGAAATTTTTATCATAGA+CGG | - | chr7.3:12612999-12613018 | MS.gene64514:intron | 15.0% |
| !! | ACAATACTAATAATAACTTC+AGG | + | chr7.3:12613660-12613679 | None:intergenic | 20.0% |
| !! | ATTAGCTAAAATTGAATGTA+AGG | + | chr7.3:12612585-12612604 | None:intergenic | 20.0% |
| !! | CGATATCAATTTATTTACAA+TGG | + | chr7.3:12613723-12613742 | None:intergenic | 20.0% |
| !! | TAAATAAGATATAGATGTCT+TGG | - | chr7.3:12612966-12612985 | MS.gene64514:intron | 20.0% |
| !! | TATCATGTTTCATTTAGAAA+AGG | - | chr7.3:12614059-12614078 | MS.gene64514:CDS | 20.0% |
| !! | TCAAAATTGAAACAAAAACT+AGG | - | chr7.3:12612693-12612712 | MS.gene64514:intron | 20.0% |
| !! | TTTACAACAGAAAATTCATT+CGG | + | chr7.3:12613629-12613648 | None:intergenic | 20.0% |
| !!! | ATTCTAGTTGATTTATACAA+AGG | - | chr7.3:12613492-12613511 | MS.gene64514:intron | 20.0% |
| !!! | TTGAAATTTTTATCATAGAC+GGG | - | chr7.3:12613000-12613019 | MS.gene64514:intron | 20.0% |
| ! | AGAAACAAAAAATCAGTGTT+TGG | + | chr7.3:12614000-12614019 | None:intergenic | 25.0% |
| ! | ATAGTTGACAATAATAACAC+AGG | + | chr7.3:12613113-12613132 | None:intergenic | 25.0% |
| ! | GAAGAAAACAATAGACATTA+TGG | + | chr7.3:12613951-12613970 | None:intergenic | 25.0% |
| ! | GTTAGTTACCAAAAACATAT+AGG | - | chr7.3:12613289-12613308 | MS.gene64514:intron | 25.0% |
| ! | TACTGTATATCAATACAACA+TGG | - | chr7.3:12613176-12613195 | MS.gene64514:CDS | 25.0% |
| ! | TAGTTGACAATAATAACACA+GGG | + | chr7.3:12613112-12613131 | None:intergenic | 25.0% |
| ! | TATACAGTAATGAAAGAAAG+CGG | + | chr7.3:12613165-12613184 | None:intergenic | 25.0% |
| !!! | AGAAAAGGTACATTTTATGT+TGG | - | chr7.3:12614074-12614093 | MS.gene64514:CDS | 25.0% |
| !!! | ATTTATTCAACCCTAGTTTT+AGG | - | chr7.3:12612625-12612644 | MS.gene64514:intron | 25.0% |
| !!! | TTTATTCAACCCTAGTTTTA+GGG | - | chr7.3:12612626-12612645 | MS.gene64514:intron | 25.0% |
| AATCATTGACCTAATACCTT+AGG | + | chr7.3:12613202-12613221 | None:intergenic | 30.0% | |
| AATCTATCATGGAGAACTTA+TGG | + | chr7.3:12612813-12612832 | None:intergenic | 30.0% | |
| AATTGATATCGCTTCAAATC+TGG | - | chr7.3:12613732-12613751 | MS.gene64514:intron | 30.0% | |
| AGTTGACAATAATAACACAG+GGG | + | chr7.3:12613111-12613130 | None:intergenic | 30.0% | |
| ATTGTTCATTCTAACTTCTG+TGG | - | chr7.3:12613765-12613784 | MS.gene64514:intron | 30.0% | |
| CTAAAGCCAATTTCAGAATA+TGG | + | chr7.3:12613977-12613996 | None:intergenic | 30.0% | |
| GACATTATGGAAGTAATGTA+AGG | + | chr7.3:12613938-12613957 | None:intergenic | 30.0% | |
| GCAACTACAAAATTGTATAC+AGG | - | chr7.3:12613399-12613418 | MS.gene64514:intron | 30.0% | |
| TAAGATATAGATGTCTTGGA+AGG | - | chr7.3:12612970-12612989 | MS.gene64514:intron | 30.0% | |
| TTACAATGGAAGTGATACAT+GGG | + | chr7.3:12613709-12613728 | None:intergenic | 30.0% | |
| TTTACAATGGAAGTGATACA+TGG | + | chr7.3:12613710-12613729 | None:intergenic | 30.0% | |
| ! | ACATTTTATGTTGGAGGAAA+TGG | - | chr7.3:12614083-12614102 | MS.gene64514:CDS | 30.0% |
| ! | CAACTGAACCTATATGTTTT+TGG | + | chr7.3:12613300-12613319 | None:intergenic | 30.0% |
| ! | CTAGTTGATTTATACAAAGG+TGG | - | chr7.3:12613495-12613514 | MS.gene64514:intron | 30.0% |
| ! | GCTGATTTCATAATCTATCA+TGG | + | chr7.3:12612824-12612843 | None:intergenic | 30.0% |
| ! | GTTTTATCCATAAGCTGAAT+AGG | + | chr7.3:12614156-12614175 | None:intergenic | 30.0% |
| !! | AAAGGTACATTTTATGTTGG+AGG | - | chr7.3:12614077-12614096 | MS.gene64514:CDS | 30.0% |
| !! | TTCCTTCCATATTCTGAAAT+TGG | - | chr7.3:12613968-12613987 | MS.gene64514:intron | 30.0% |
| !!! | CTATGCATCTTTGTTTTTTC+TGG | - | chr7.3:12613424-12613443 | MS.gene64514:intron | 30.0% |
| AGCCAATTTCAGAATATGGA+AGG | + | chr7.3:12613973-12613992 | None:intergenic | 35.0% | |
| CATAAACACTTGTGAGACTA+TGG | - | chr7.3:12612745-12612764 | MS.gene64514:CDS | 35.0% | |
| GGAAAAGAAAGTAGTTAGTG+AGG | - | chr7.3:12613062-12613081 | MS.gene64514:intron | 35.0% | |
| GTGTTAATGGTGAACAAACA+AGG | - | chr7.3:12612413-12612432 | MS.gene64514:CDS | 35.0% | |
| TATCAATACAACATGGCCTA+AGG | - | chr7.3:12613183-12613202 | MS.gene64514:intron | 35.0% | |
| TGTTCACCATTAACACGAAT+CGG | + | chr7.3:12612409-12612428 | None:intergenic | 35.0% | |
| ! | AGGTTCAGTTGTCTTATTGT+TGG | - | chr7.3:12613309-12613328 | MS.gene64514:intron | 35.0% |
| ! | CTACTTTCTTTTCCCCAATT+TGG | + | chr7.3:12613056-12613075 | None:intergenic | 35.0% |
| ! | TGGCTGATACATTTTTGTCA+TGG | - | chr7.3:12613353-12613372 | MS.gene64514:intron | 35.0% |
| !! | AGCATACCTGGTTTTCATTA+CGG | + | chr7.3:12612526-12612545 | None:intergenic | 35.0% |
| !!! | GCATCTTTGTTTTTTCTGGT+TGG | - | chr7.3:12613428-12613447 | MS.gene64514:intron | 35.0% |
| ATTACGCTGAAGCTGAAATG+TGG | + | chr7.3:12613684-12613703 | None:intergenic | 40.0% | |
| CATAGACGGGAGAAAATAGT+TGG | - | chr7.3:12613013-12613032 | MS.gene64514:intron | 40.0% | |
| GGTCATGAATGGTTGTATTG+TGG | - | chr7.3:12614104-12614123 | MS.gene64514:CDS | 40.0% | |
| GTCATGGCTTCAAATGTGAT+CGG | - | chr7.3:12613579-12613598 | MS.gene64514:intron | 40.0% | |
| TCGGTTTCTTTAGACAAGCA+AGG | + | chr7.3:12613610-12613629 | None:intergenic | 40.0% | |
| TCTGACTCCTATTCAGCTTA+TGG | - | chr7.3:12614146-12614165 | MS.gene64514:CDS | 40.0% | |
| TGTATCAGCCAGATCAATCA+AGG | + | chr7.3:12613344-12613363 | None:intergenic | 40.0% | |
| TTGGAGGAAATGGTCATGAA+TGG | - | chr7.3:12614093-12614112 | MS.gene64514:CDS | 40.0% | |
| ! | ACAACATGGCCTAAGGTATT+AGG | - | chr7.3:12613190-12613209 | MS.gene64514:intron | 40.0% |
| ! | CTTTCTTTTCCCCAATTTGG+CGG | + | chr7.3:12613053-12613072 | None:intergenic | 40.0% |
| ! | GAAAAGAATGGAAGCATACC+TGG | + | chr7.3:12612538-12612557 | None:intergenic | 40.0% |
| ! | GAAACCATGGATCGACATTT+TGG | - | chr7.3:12613839-12613858 | MS.gene64514:intron | 40.0% |
| ! | TTTCTTTTCCCCAATTTGGC+GGG | + | chr7.3:12613052-12613071 | None:intergenic | 40.0% |
| !! | GCATACCTGGTTTTCATTAC+GGG | + | chr7.3:12612525-12612544 | None:intergenic | 40.0% |
| !! | GTTTTGATCTAACTGACCAG+TGG | - | chr7.3:12613557-12613576 | MS.gene64514:intron | 40.0% |
| !! | TTTCTGGTTGGAGTTGATGA+CGG | - | chr7.3:12613440-12613459 | MS.gene64514:intron | 40.0% |
| !!! | GCAGAATGAGCTTGCTATTT+TGG | - | chr7.3:12613796-12613815 | MS.gene64514:intron | 40.0% |
| ACCTCACTGTTGAAGAACGA+CGG | - | chr7.3:12612465-12612484 | MS.gene64514:CDS | 45.0% | |
| ACGAACGATTTCGCCTTCAA+GGG | + | chr7.3:12612492-12612511 | None:intergenic | 45.0% | |
| ATCTAACTGACCAGTGGTCA+TGG | - | chr7.3:12613563-12613582 | MS.gene64514:intron | 45.0% | |
| CCAAGCATGCCCTAAAACTA+GGG | + | chr7.3:12612638-12612657 | None:intergenic | 45.0% | |
| CCATCTCTTAGTCGAAACCA+TGG | - | chr7.3:12613826-12613845 | MS.gene64514:intron | 45.0% | |
| CCATGGTTTCGACTAAGAGA+TGG | + | chr7.3:12613829-12613848 | None:intergenic | 45.0% | |
| CTGCTGTGACTAAGAGTCTT+TGG | - | chr7.3:12613876-12613895 | MS.gene64514:intron | 45.0% | |
| GCCAGATCAATCAAGGTGAA+CGG | + | chr7.3:12613337-12613356 | None:intergenic | 45.0% | |
| GTTGCCAAAATGTCGATCCA+TGG | + | chr7.3:12613846-12613865 | None:intergenic | 45.0% | |
| TACGAACGATTTCGCCTTCA+AGG | + | chr7.3:12612493-12612512 | None:intergenic | 45.0% | |
| TCCAAGCATGCCCTAAAACT+AGG | + | chr7.3:12612639-12612658 | None:intergenic | 45.0% | |
| TCCGTTCACCTTGATTGATC+TGG | - | chr7.3:12613333-12613352 | MS.gene64514:intron | 45.0% | |
| ! | ACATTTGAAGCCATGACCAC+TGG | + | chr7.3:12613576-12613595 | None:intergenic | 45.0% |
| ! | TTCTTTTCCCCAATTTGGCG+GGG | + | chr7.3:12613051-12613070 | None:intergenic | 45.0% |
| !! | TTAATAAAATAAAAAACTTA+GGG | - | chr7.3:12613134-12613153 | MS.gene64514:CDS | 5.0% |
| CTTTCACACCCCGCCAAATT+GGG | - | chr7.3:12613040-12613059 | MS.gene64514:intron | 50.0% | |
| TCCGTCGTTCTTCAACAGTG+AGG | + | chr7.3:12612469-12612488 | None:intergenic | 50.0% | |
| TTTCACACCCCGCCAAATTG+GGG | - | chr7.3:12613041-12613060 | MS.gene64514:intron | 50.0% | |
| ! | GGGGATCCGATTCGTGTTAA+TGG | - | chr7.3:12612400-12612419 | MS.gene64514:CDS | 50.0% |
| !! | GGATCGACATTTTGGCAACG+TGG | - | chr7.3:12613847-12613866 | MS.gene64514:intron | 50.0% |
| !!! | CCCTAGTTTTAGGGCATGCT+TGG | - | chr7.3:12612635-12612654 | MS.gene64514:intron | 50.0% |
| CCAATTTGGCGGGGTGTGAA+AGG | + | chr7.3:12613042-12613061 | None:intergenic | 55.0% | |
| CCTCGCCCGTAATGAAAACC+AGG | - | chr7.3:12612517-12612536 | MS.gene64514:intron | 55.0% | |
| CCTTTCACACCCCGCCAAAT+TGG | - | chr7.3:12613039-12613058 | MS.gene64514:intron | 55.0% | |
| GAAGAACGACGGACCCTTGA+AGG | - | chr7.3:12612476-12612495 | MS.gene64514:CDS | 55.0% | |
| GGGCATGCTTGGATCATCGA+AGG | - | chr7.3:12612646-12612665 | MS.gene64514:intron | 55.0% | |
| !! | CCTGGTTTTCATTACGGGCG+AGG | + | chr7.3:12612520-12612539 | None:intergenic | 55.0% |
| !! | GGTTGGAGTTGATGACGGCG+AGG | - | chr7.3:12613445-12613464 | MS.gene64514:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 12612398 | 12614182 | 12612398 | ID=MS.gene64514 |
| chr7.3 | mRNA | 12612398 | 12614182 | 12612398 | ID=MS.gene64514.t1;Parent=MS.gene64514 |
| chr7.3 | exon | 12614042 | 12614182 | 12614042 | ID=MS.gene64514.t1.exon1;Parent=MS.gene64514.t1 |
| chr7.3 | CDS | 12614042 | 12614182 | 12614042 | ID=cds.MS.gene64514.t1;Parent=MS.gene64514.t1 |
| chr7.3 | exon | 12613114 | 12613200 | 12613114 | ID=MS.gene64514.t1.exon2;Parent=MS.gene64514.t1 |
| chr7.3 | CDS | 12613114 | 12613200 | 12613114 | ID=cds.MS.gene64514.t1;Parent=MS.gene64514.t1 |
| chr7.3 | exon | 12612712 | 12612780 | 12612712 | ID=MS.gene64514.t1.exon3;Parent=MS.gene64514.t1 |
| chr7.3 | CDS | 12612712 | 12612780 | 12612712 | ID=cds.MS.gene64514.t1;Parent=MS.gene64514.t1 |
| chr7.3 | exon | 12612398 | 12612532 | 12612398 | ID=MS.gene64514.t1.exon4;Parent=MS.gene64514.t1 |
| chr7.3 | CDS | 12612398 | 12612532 | 12612398 | ID=cds.MS.gene64514.t1;Parent=MS.gene64514.t1 |
| Gene Sequence |
| Protein sequence |
