Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64659.t1 | XP_003616397.1 | 98.5 | 391 | 6 | 0 | 1 | 391 | 1 | 391 | 1.00E-222 | 782.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64659.t1 | Q9Y312 | 28.8 | 400 | 245 | 9 | 1 | 386 | 6 | 379 | 4.3e-36 | 153.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64659.t1 | G7KBQ2 | 98.5 | 391 | 6 | 0 | 1 | 391 | 1 | 391 | 7.4e-223 | 782.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene05191 | MS.gene64659 | 0.801486 | 8.80E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene68979 | MS.gene64659 | PPI |
| MS.gene038800 | MS.gene64659 | PPI |
| MS.gene033017 | MS.gene64659 | PPI |
| MS.gene044498 | MS.gene64659 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64659.t1 | MTR_5g079770 | 98.465 | 391 | 6 | 0 | 1 | 391 | 1 | 391 | 0.0 | 798 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64659.t1 | AT1G66510 | 67.506 | 397 | 117 | 5 | 1 | 390 | 1 | 392 | 0.0 | 560 |
| MS.gene64659.t1 | AT1G66510 | 67.506 | 397 | 117 | 5 | 1 | 390 | 1 | 392 | 0.0 | 560 |
| MS.gene64659.t1 | AT1G66510 | 67.506 | 397 | 117 | 5 | 1 | 390 | 1 | 392 | 0.0 | 560 |
| MS.gene64659.t1 | AT1G66510 | 65.722 | 353 | 109 | 5 | 45 | 390 | 1 | 348 | 1.51e-172 | 486 |
Find 84 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCAGGTAATTGTTCGTAAA+TGG | 0.186934 | 5.2:+67807391 | MS.gene64659:intron |
| ACTTTACTGGCAAAAGATTA+TGG | 0.257738 | 5.2:+67809446 | MS.gene64659:CDS |
| CACTATTGTTGGATGATTCT+TGG | 0.258501 | 5.2:+67811025 | MS.gene64659:CDS |
| AAGCTATTAGAGAGCAATCT+AGG | 0.318940 | 5.2:+67811675 | MS.gene64659:CDS |
| GTTTGCATTTATCGCCCTTA+TGG | 0.327929 | 5.2:+67809502 | MS.gene64659:CDS |
| CAGCAGAACAACGCTGTTGA+TGG | 0.346353 | 5.2:+67811708 | MS.gene64659:CDS |
| TCCTTGGTTAGCCTTCTGTT+TGG | 0.383792 | 5.2:+67809638 | MS.gene64659:CDS |
| ATGGAATTTGACCGACAACT+TGG | 0.429503 | 5.2:+67808189 | MS.gene64659:CDS |
| CTAGATGGGTCTGTCATTGA+TGG | 0.429928 | 5.2:+67811462 | MS.gene64659:CDS |
| ACAATTGCTAGAGACTTTAC+TGG | 0.432701 | 5.2:+67809433 | MS.gene64659:CDS |
| TGTTTATAGGTGTTCTCTGT+GGG | 0.433030 | 5.2:+67804860 | MS.gene64659:intron |
| ATTGGATTCTTTATCGATGC+TGG | 0.440297 | 5.2:+67805895 | MS.gene64659:CDS |
| TGATTAGCCGTTCCTCCTGT+TGG | 0.446796 | 5.2:-67807416 | None:intergenic |
| ATCTTAGCCACTATGAAGAT+TGG | 0.447384 | 5.2:+67808220 | MS.gene64659:CDS |
| TAGTAGACAAAATGAGTTCC+CGG | 0.450062 | 5.2:-67804914 | None:intergenic |
| TGTTCGTAAATGGGACCAAC+AGG | 0.453079 | 5.2:+67807401 | MS.gene64659:CDS |
| GTTGGCAAGTTGCAGAGAAA+AGG | 0.455685 | 5.2:+67808486 | MS.gene64659:CDS |
| GCAGGTAATTGTTCGTAAAT+GGG | 0.457276 | 5.2:+67807392 | MS.gene64659:intron |
| ATCTATTATCAACTAAAATA+CGG | 0.457832 | 5.2:+67810970 | MS.gene64659:CDS |
| GGTTCTGAAGACTTGCTCCT+TGG | 0.470331 | 5.2:+67809470 | MS.gene64659:CDS |
| TCATAGTGGCTAAGATTGTA+AGG | 0.472984 | 5.2:-67808213 | None:intergenic |
| GTGTCAAGTGCTTTCTCCAT+TGG | 0.475193 | 5.2:-67808432 | None:intergenic |
| AACACTGACTTCTCCTCCAA+TGG | 0.477819 | 5.2:-67808370 | None:intergenic |
| AGAAAATATACTTACTGCTT+CGG | 0.477936 | 5.2:-67809666 | None:intergenic |
| GATAATTCTGCAACATCTGT+TGG | 0.478381 | 5.2:+67808468 | MS.gene64659:CDS |
| CTAGGATGAAAGTTATAGTC+TGG | 0.482454 | 5.2:+67808155 | MS.gene64659:intron |
| GGGTATTAAGATGATTCCTC+CGG | 0.487283 | 5.2:+67804895 | MS.gene64659:CDS |
| ATTTCTCCAGTGGAAATCCT+TGG | 0.497323 | 5.2:+67809622 | MS.gene64659:CDS |
| GATTGTAAGGCCCAAGTTGT+CGG | 0.506606 | 5.2:-67808200 | None:intergenic |
| TAGCAACAAGGGTGTATTGA+GGG | 0.510487 | 5.2:-67802916 | None:intergenic |
| AATCAAAGTATCTGAGGAAG+AGG | 0.515995 | 5.2:+67807434 | MS.gene64659:CDS |
| TAAATAATACCTTACAATGA+TGG | 0.519108 | 5.2:-67811071 | None:intergenic |
| TTGGCAAGTTGCAGAGAAAA+GGG | 0.521046 | 5.2:+67808487 | MS.gene64659:CDS |
| GATCAAATCAAACCTGGTTG+AGG | 0.524670 | 5.2:-67804942 | None:intergenic |
| TCATCTAACATCTCTACTAC+AGG | 0.528298 | 5.2:-67811851 | None:intergenic |
| CCTTGTTGCTATTGATACTC+AGG | 0.530604 | 5.2:+67802928 | MS.gene64659:CDS |
| TAACACAGGACCACTATTGT+TGG | 0.533398 | 5.2:+67811014 | MS.gene64659:CDS |
| TTGTTTATAGGTGTTCTCTG+TGG | 0.534093 | 5.2:+67804859 | MS.gene64659:intron |
| TGGAATTTGACCGACAACTT+GGG | 0.538353 | 5.2:+67808190 | MS.gene64659:CDS |
| ACACTGACTTCTCCTCCAAT+GGG | 0.539146 | 5.2:-67808369 | None:intergenic |
| CAAGAATCATCCAACAATAG+TGG | 0.539982 | 5.2:-67811024 | None:intergenic |
| GGCTTCCTGCAGAGACGGAA+AGG | 0.542700 | 5.2:+67805858 | MS.gene64659:intron |
| GGCTAACCAAGGATTTCCAC+TGG | 0.549600 | 5.2:-67809628 | None:intergenic |
| ATCTTAATACCCTTAAAAGA+AGG | 0.553188 | 5.2:-67804884 | None:intergenic |
| TATCGATGCTGGCCCTTCAG+AGG | 0.553264 | 5.2:+67805906 | MS.gene64659:CDS |
| TATTTATGAGCAGAGCCCAT+TGG | 0.553357 | 5.2:+67808354 | MS.gene64659:intron |
| TTATGAGCAGAGCCCATTGG+AGG | 0.557137 | 5.2:+67808357 | MS.gene64659:intron |
| ATGCAAGGGGATCAGTGGAC+AGG | 0.557605 | 5.2:+67808542 | MS.gene64659:CDS |
| AGCTATTAGAGAGCAATCTA+GGG | 0.559485 | 5.2:+67811676 | MS.gene64659:CDS |
| TCCACGTGTCGTAAAATGCA+AGG | 0.565062 | 5.2:+67808527 | MS.gene64659:CDS |
| ATCGTTTCCAATCTTCATAG+TGG | 0.569850 | 5.2:-67808227 | None:intergenic |
| AAGAGCATCATTGAACGGCT+TGG | 0.570048 | 5.2:+67808267 | MS.gene64659:CDS |
| CGTTAGAAGCATTTCTCCAG+TGG | 0.577996 | 5.2:+67809612 | MS.gene64659:CDS |
| TCTACTATAGTTCCTCAACC+AGG | 0.581273 | 5.2:+67804930 | MS.gene64659:CDS |
| CCTGAGTATCAATAGCAACA+AGG | 0.581885 | 5.2:-67802928 | None:intergenic |
| CCACGTGTCGTAAAATGCAA+GGG | 0.583315 | 5.2:+67808528 | MS.gene64659:CDS |
| ATAGCAACAAGGGTGTATTG+AGG | 0.586557 | 5.2:-67802917 | None:intergenic |
| TTACAAAGAGCATCATTGAA+CGG | 0.590572 | 5.2:+67808262 | MS.gene64659:CDS |
| AGCACTTGACACGCAGCTGA+AGG | 0.595933 | 5.2:+67808443 | MS.gene64659:CDS |
| CTTCGGTGCAGCCAAACAGA+AGG | 0.598687 | 5.2:-67809649 | None:intergenic |
| AAACGCTCCTAAAACACCAA+TGG | 0.600774 | 5.2:+67808416 | MS.gene64659:CDS |
| TATTAGAGAGCAATCTAGGG+TGG | 0.601228 | 5.2:+67811679 | MS.gene64659:CDS |
| TCGATAAAGAATCCAATCAT+CGG | 0.602243 | 5.2:-67805889 | None:intergenic |
| AATGGGACCAACAGGAGGAA+CGG | 0.609015 | 5.2:+67807409 | MS.gene64659:CDS |
| GGTATTAAGATGATTCCTCC+GGG | 0.612119 | 5.2:+67804896 | MS.gene64659:CDS |
| GTAAAATGCAAGGGGATCAG+TGG | 0.612809 | 5.2:+67808537 | MS.gene64659:CDS |
| ATTAGAGAGCAATCTAGGGT+GGG | 0.617313 | 5.2:+67811680 | MS.gene64659:CDS |
| ATGCAAACTGCAGTTCTCCA+AGG | 0.619224 | 5.2:-67809487 | None:intergenic |
| ACGGCTAATCAAAGTATCTG+AGG | 0.620148 | 5.2:+67807428 | MS.gene64659:CDS |
| AGCCGTGTTCGTGTGTGGAA+AGG | 0.621192 | 5.2:-67810397 | None:intergenic |
| CTGAGTATCAATAGCAACAA+GGG | 0.621644 | 5.2:-67802927 | None:intergenic |
| AGCCTTTCCACACACGAACA+CGG | 0.635124 | 5.2:+67810395 | MS.gene64659:intron |
| TGAATAGCCGTGTTCGTGTG+TGG | 0.635232 | 5.2:-67810402 | None:intergenic |
| CGGACTACAGAAAGATCGCA+AGG | 0.640325 | 5.2:+67810990 | MS.gene64659:CDS |
| TACTTCTTTGAATCTTGACA+AGG | 0.647721 | 5.2:+67808569 | MS.gene64659:CDS |
| CACGTGTCGTAAAATGCAAG+GGG | 0.651470 | 5.2:+67808529 | MS.gene64659:CDS |
| GCCAAACAGAAGGCTAACCA+AGG | 0.675388 | 5.2:-67809639 | None:intergenic |
| GTACTTTGATGAGAACGATG+AGG | 0.679398 | 5.2:+67811734 | MS.gene64659:CDS |
| TCGTAAATGGGACCAACAGG+AGG | 0.680154 | 5.2:+67807404 | MS.gene64659:CDS |
| AAAGATCGCAAGGATAACAC+AGG | 0.694703 | 5.2:+67811000 | MS.gene64659:CDS |
| TTACTGGCAAAAGATTATGG+AGG | 0.700059 | 5.2:+67809449 | MS.gene64659:CDS |
| GGGTGTATTGAGGGACATCG+AGG | 0.700381 | 5.2:-67802907 | None:intergenic |
| ACGAACACGGCTATTCACCA+AGG | 0.734563 | 5.2:+67810408 | MS.gene64659:CDS |
| TAGACAAAATGAGTTCCCGG+AGG | 0.766673 | 5.2:-67804911 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.2 | gene | 67802854 | 67811892 | 67802854 | ID=MS.gene64659 |
| chr5.2 | mRNA | 67802854 | 67811892 | 67802854 | ID=MS.gene64659.t1;Parent=MS.gene64659 |
| chr5.2 | exon | 67802854 | 67802949 | 67802854 | ID=MS.gene64659.t1.exon1;Parent=MS.gene64659.t1 |
| chr5.2 | CDS | 67802854 | 67802949 | 67802854 | ID=cds.MS.gene64659.t1;Parent=MS.gene64659.t1 |
| chr5.2 | exon | 67804869 | 67804951 | 67804869 | ID=MS.gene64659.t1.exon2;Parent=MS.gene64659.t1 |
| chr5.2 | CDS | 67804869 | 67804951 | 67804869 | ID=cds.MS.gene64659.t1;Parent=MS.gene64659.t1 |
| chr5.2 | exon | 67805870 | 67805927 | 67805870 | ID=MS.gene64659.t1.exon3;Parent=MS.gene64659.t1 |
| chr5.2 | CDS | 67805870 | 67805927 | 67805870 | ID=cds.MS.gene64659.t1;Parent=MS.gene64659.t1 |
| chr5.2 | exon | 67807396 | 67807455 | 67807396 | ID=MS.gene64659.t1.exon4;Parent=MS.gene64659.t1 |
| chr5.2 | CDS | 67807396 | 67807455 | 67807396 | ID=cds.MS.gene64659.t1;Parent=MS.gene64659.t1 |
| chr5.2 | exon | 67808159 | 67808288 | 67808159 | ID=MS.gene64659.t1.exon5;Parent=MS.gene64659.t1 |
| chr5.2 | CDS | 67808159 | 67808288 | 67808159 | ID=cds.MS.gene64659.t1;Parent=MS.gene64659.t1 |
| chr5.2 | exon | 67808367 | 67808590 | 67808367 | ID=MS.gene64659.t1.exon6;Parent=MS.gene64659.t1 |
| chr5.2 | CDS | 67808367 | 67808590 | 67808367 | ID=cds.MS.gene64659.t1;Parent=MS.gene64659.t1 |
| chr5.2 | exon | 67809431 | 67809523 | 67809431 | ID=MS.gene64659.t1.exon7;Parent=MS.gene64659.t1 |
| chr5.2 | CDS | 67809431 | 67809523 | 67809431 | ID=cds.MS.gene64659.t1;Parent=MS.gene64659.t1 |
| chr5.2 | exon | 67809602 | 67809673 | 67809602 | ID=MS.gene64659.t1.exon8;Parent=MS.gene64659.t1 |
| chr5.2 | CDS | 67809602 | 67809673 | 67809602 | ID=cds.MS.gene64659.t1;Parent=MS.gene64659.t1 |
| chr5.2 | exon | 67810397 | 67810429 | 67810397 | ID=MS.gene64659.t1.exon9;Parent=MS.gene64659.t1 |
| chr5.2 | CDS | 67810397 | 67810429 | 67810397 | ID=cds.MS.gene64659.t1;Parent=MS.gene64659.t1 |
| chr5.2 | exon | 67810958 | 67811083 | 67810958 | ID=MS.gene64659.t1.exon10;Parent=MS.gene64659.t1 |
| chr5.2 | CDS | 67810958 | 67811083 | 67810958 | ID=cds.MS.gene64659.t1;Parent=MS.gene64659.t1 |
| chr5.2 | exon | 67811444 | 67811500 | 67811444 | ID=MS.gene64659.t1.exon11;Parent=MS.gene64659.t1 |
| chr5.2 | CDS | 67811444 | 67811500 | 67811444 | ID=cds.MS.gene64659.t1;Parent=MS.gene64659.t1 |
| chr5.2 | exon | 67811660 | 67811755 | 67811660 | ID=MS.gene64659.t1.exon12;Parent=MS.gene64659.t1 |
| chr5.2 | CDS | 67811660 | 67811755 | 67811660 | ID=cds.MS.gene64659.t1;Parent=MS.gene64659.t1 |
| chr5.2 | exon | 67811845 | 67811892 | 67811845 | ID=MS.gene64659.t1.exon13;Parent=MS.gene64659.t1 |
| chr5.2 | CDS | 67811845 | 67811892 | 67811845 | ID=cds.MS.gene64659.t1;Parent=MS.gene64659.t1 |
| Gene Sequence |
| Protein sequence |
