AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene05191


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene05191.t1 MTR_7g024300 50.000 80 37 1 1 77 28 107 1.79e-18 77.0
MS.gene05191.t1 MTR_7g024280 46.250 80 40 1 1 77 27 106 5.62e-15 68.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene05191.t1 AT1G65520 49.206 63 28 1 11 69 38 100 1.81e-11 58.5

Find 30 sgRNAs with CRISPR-Local

Find 115 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGTGTGAACTTGGGTAAAT+AGG 0.317284 6.4:+52260865 None:intergenic
GTATGTTACTTCAATATTCC+AGG 0.392807 6.4:+52260841 None:intergenic
ATTCCAGGATTGTGTGAACT+TGG 0.431318 6.4:+52260856 None:intergenic
CACCGTCACGCCTTCGCCGC+AGG 0.455545 6.4:-52262677 MS.gene05191:intron
GAAGGCGGCGATGGTTGGCA+TGG 0.457955 6.4:+52262699 None:intergenic
CGGCGAAGGCGTGACGGTGA+AGG 0.465129 6.4:+52262681 None:intergenic
ACGGTGAAGGCGGCGATGGT+TGG 0.471893 6.4:+52262694 None:intergenic
TTGATTGAATTGAGAAGTGT+GGG 0.494586 6.4:+52262863 None:intergenic
TACCCAAGTTCACACAATCC+TGG 0.508621 6.4:-52260859 MS.gene05191:CDS
AAGGCGGCGATGGTTGGCAT+GGG 0.512341 6.4:+52262700 None:intergenic
GTAACATACTACATGAGTTA+CGG 0.526123 6.4:-52260827 MS.gene05191:CDS
CGTGACGGTGAAGGCGGCGA+TGG 0.531005 6.4:+52262690 None:intergenic
CATGGGATGAGAGAGAAGTT+CGG 0.543922 6.4:+52262717 None:intergenic
TTCCAGGATTGTGTGAACTT+GGG 0.555975 6.4:+52260857 None:intergenic
GAGGATGTAACCTGCGGCGA+AGG 0.566868 6.4:+52262667 None:intergenic
TTTGATTGAATTGAGAAGTG+TGG 0.568432 6.4:+52262862 None:intergenic
CAACAGCTACGACATCGATC+GGG 0.568492 6.4:-52262797 MS.gene05191:CDS
CCGATCGATGTCGTAGCTGT+TGG 0.575849 6.4:+52262798 None:intergenic
CGAAGGCGTGACGGTGAAGG+CGG 0.577796 6.4:+52262684 None:intergenic
CCAACAGCTACGACATCGAT+CGG 0.588580 6.4:-52262798 MS.gene05191:CDS
GGTGATGAAGACGAGGAGGA+CGG 0.623893 6.4:+52262838 None:intergenic
AACCTGCGGCGAAGGCGTGA+CGG 0.631674 6.4:+52262675 None:intergenic
GTTCGGAGACAACAGAGCGG+AGG 0.635659 6.4:+52262734 None:intergenic
AGCGGAGGCGATAGTCGATG+AGG 0.674566 6.4:+52262749 None:intergenic
GCGCGGTGGTGATGAAGACG+AGG 0.682967 6.4:+52262831 None:intergenic
CGGTGGTGATGAAGACGAGG+AGG 0.690375 6.4:+52262834 None:intergenic
TGATTGAATTGAGAAGTGTG+GGG 0.704818 6.4:+52262864 None:intergenic
TTGGAGAAGAATTTGCGCGG+TGG 0.714981 6.4:+52262817 None:intergenic
CTGTTGGAGAAGAATTTGCG+CGG 0.716388 6.4:+52262814 None:intergenic
GAAGTTCGGAGACAACAGAG+CGG 0.757254 6.4:+52262731 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTGTATTTTTTTTTCAGTT+GGG + chr6.4:52261184-52261203 None:intergenic 15.0%
!!! TTTTGTATTTTTTTTTCAGT+TGG + chr6.4:52261185-52261204 None:intergenic 15.0%
!! CAATTATCTTCAGTTAAATA+TGG + chr6.4:52261773-52261792 None:intergenic 20.0%
!! TTATATATGTCAATCCAAAA+AGG + chr6.4:52261304-52261323 None:intergenic 20.0%
!! TTCAAATTATTATGTTGTTG+AGG - chr6.4:52262290-52262309 MS.gene05191:intron 20.0%
!!! ATTTCTCTTATTGTGTTTTT+TGG - chr6.4:52261446-52261465 MS.gene05191:intron 20.0%
!!! CTTTTTTCTTTTATATTCTG+TGG - chr6.4:52262558-52262577 MS.gene05191:intron 20.0%
!!! TATATAAACAGACATGTTTT+TGG - chr6.4:52261375-52261394 MS.gene05191:intron 20.0%
!!! TGTTTCTTTTGATCAAATTT+AGG - chr6.4:52261884-52261903 MS.gene05191:intron 20.0%
!!! TTCTGATTATTTTTTGTTGT+AGG - chr6.4:52262791-52262810 MS.gene05191:CDS 20.0%
! AAAGAAACAAATCTTTCCAT+TGG + chr6.4:52261873-52261892 None:intergenic 25.0%
! AAAGAAATAGATAAGACTGA+AGG - chr6.4:52261577-52261596 MS.gene05191:intron 25.0%
! AAGCACAAACTTAAACATAT+TGG + chr6.4:52262533-52262552 None:intergenic 25.0%
! AAGTTCATATAAAAGTTTGC+AGG - chr6.4:52262201-52262220 MS.gene05191:intron 25.0%
! AATTGATATACCAAGAATTG+TGG + chr6.4:52262679-52262698 None:intergenic 25.0%
! ACAAATTGCATTACTATAGT+TGG + chr6.4:52261731-52261750 None:intergenic 25.0%
! ACATGAAAAAGTTACAGATA+TGG + chr6.4:52261075-52261094 None:intergenic 25.0%
! CAAGAAATCATGAATTCAAA+AGG + chr6.4:52261111-52261130 None:intergenic 25.0%
! CAATCATAAAAAACTGCATT+TGG + chr6.4:52261613-52261632 None:intergenic 25.0%
! CTACTTCAATTTACAATACA+AGG - chr6.4:52261956-52261975 MS.gene05191:intron 25.0%
! TCAATTTACAATACAAGGTT+GGG - chr6.4:52261961-52261980 MS.gene05191:intron 25.0%
! TGTTGTTCTGTTATATTGAT+TGG - chr6.4:52261519-52261538 MS.gene05191:intron 25.0%
! TTCAATTTACAATACAAGGT+TGG - chr6.4:52261960-52261979 MS.gene05191:intron 25.0%
!! AAATCCTAACTCTGAAAAAT+TGG + chr6.4:52261221-52261240 None:intergenic 25.0%
!! CTTTTGAATTCATGATTTCT+TGG - chr6.4:52261109-52261128 MS.gene05191:intron 25.0%
!! TTTTGGTATTTAATAGCCAA+TGG - chr6.4:52261854-52261873 MS.gene05191:intron 25.0%
!!! ATTTTTTGTTGTAGGCATTT+CGG - chr6.4:52262799-52262818 MS.gene05191:CDS 25.0%
!!! CAGTTTTTTCTACATGAAAT+TGG + chr6.4:52262434-52262453 None:intergenic 25.0%
!!! TTCTGTGTGATTTTTTTAGA+AGG - chr6.4:52261336-52261355 MS.gene05191:intron 25.0%
ACAACATCAATGACAAAATG+AGG + chr6.4:52262353-52262372 None:intergenic 30.0%
ACATAAAGAAAAGATATGCG+AGG + chr6.4:52261049-52261068 None:intergenic 30.0%
ATAACAGAACAACACTTCTT+TGG + chr6.4:52261513-52261532 None:intergenic 30.0%
CAAACTTAAACATATTGGCA+TGG + chr6.4:52262528-52262547 None:intergenic 30.0%
CTGAAAAATTGGAAAGAGAA+TGG + chr6.4:52261210-52261229 None:intergenic 30.0%
CTTGAAAAGAACTCAATTGA+AGG + chr6.4:52262629-52262648 None:intergenic 30.0%
GTAACATACTACATGAGTTA+CGG - chr6.4:52262867-52262886 MS.gene05191:CDS 30.0%
TAAAAAACTGCATTTGGTTG+TGG + chr6.4:52261607-52261626 None:intergenic 30.0%
TCATGTAGAAAAAACTGATG+TGG - chr6.4:52262437-52262456 MS.gene05191:intron 30.0%
TGAGAGTTGTAATGCATTAT+AGG - chr6.4:52261702-52261721 MS.gene05191:intron 30.0%
TGCGTATATATGAAAGTTCA+AGG + chr6.4:52261643-52261662 None:intergenic 30.0%
TTAGTGAACCAAAACAGATA+AGG + chr6.4:52261821-52261840 None:intergenic 30.0%
TTTGATTGAATTGAGAAGTG+TGG + chr6.4:52260835-52260854 None:intergenic 30.0%
! ATTTTTCCTAAACGTTGCTT+TGG - chr6.4:52262653-52262672 MS.gene05191:intron 30.0%
! GTATGTTACTTCAATATTCC+AGG + chr6.4:52262856-52262875 None:intergenic 30.0%
! TAATGTTGCCTTATCTGTTT+TGG - chr6.4:52261810-52261829 MS.gene05191:intron 30.0%
! TTGATTGAATTGAGAAGTGT+GGG + chr6.4:52260834-52260853 None:intergenic 30.0%
!! AAGTTTGTTGAATTTAACGC+AGG - chr6.4:52261277-52261296 MS.gene05191:intron 30.0%
!! ACTCATTTTAATACCAAGCT+AGG + chr6.4:52261676-52261695 None:intergenic 30.0%
!! AGTTTGTTGAATTTAACGCA+GGG - chr6.4:52261278-52261297 MS.gene05191:intron 30.0%
!! CAAGTTAAAGTATTTTCTCC+AGG + chr6.4:52261417-52261436 None:intergenic 30.0%
!! CAGGTAAAAAGTTCTAAGAA+TGG - chr6.4:52262220-52262239 MS.gene05191:intron 30.0%
!! CTTTCCAATTTTTCAGAGTT+AGG - chr6.4:52261214-52261233 MS.gene05191:intron 30.0%
!! GTCATTGTGTTTGATTTGTA+TGG - chr6.4:52261922-52261941 MS.gene05191:intron 30.0%
!!! CTAATTCTCTGGTTTTGTTT+TGG - chr6.4:52261837-52261856 MS.gene05191:intron 30.0%
!!! GTGTTTTTTGGACATTTGAT+TGG - chr6.4:52261458-52261477 MS.gene05191:intron 30.0%
AAGGCAACATTAGTCATTTG+TGG + chr6.4:52261802-52261821 None:intergenic 35.0%
CATTCTTCAGCCAAAATCAA+TGG + chr6.4:52261548-52261567 None:intergenic 35.0%
GAACAGAGAGTAATAAGCTT+TGG + chr6.4:52262331-52262350 None:intergenic 35.0%
TCAGAGTTAGGATTTGAAGA+TGG - chr6.4:52261226-52261245 MS.gene05191:intron 35.0%
TTGTGTGAACTTGGGTAAAT+AGG + chr6.4:52262832-52262851 None:intergenic 35.0%
! GGTTTGTTTACTTGAAAACC+TGG - chr6.4:52261396-52261415 MS.gene05191:intron 35.0%
! TGATTGAATTGAGAAGTGTG+GGG + chr6.4:52260833-52260852 None:intergenic 35.0%
!!! GTTTTGGTTCACTAATTCTC+TGG - chr6.4:52261826-52261845 MS.gene05191:intron 35.0%
!!! TGATTGGAAGCCATTGATTT+TGG - chr6.4:52261535-52261554 MS.gene05191:intron 35.0%
ATTCCAGGATTGTGTGAACT+TGG + chr6.4:52262841-52262860 None:intergenic 40.0%
CTGTGGTTCATGAATCAAAC+TGG - chr6.4:52262575-52262594 MS.gene05191:intron 40.0%
TTCCAGGATTGTGTGAACTT+GGG + chr6.4:52262840-52262859 None:intergenic 40.0%
! AATTTAACGCAGGGCCTTTT+TGG - chr6.4:52261287-52261306 MS.gene05191:intron 40.0%
! TGTCATGTGCTGTTATCTGT+GGG - chr6.4:52262110-52262129 MS.gene05191:intron 40.0%
!! TGCTGCTGTTTGTGTTTTGT+GGG - chr6.4:52262136-52262155 MS.gene05191:intron 40.0%
ATATGCGAGGATGTAACCTG+CGG + chr6.4:52261036-52261055 None:intergenic 45.0%
CATGGGATGAGAGAGAAGTT+CGG + chr6.4:52260980-52260999 None:intergenic 45.0%
CTGTTGGAGAAGAATTTGCG+CGG + chr6.4:52260883-52260902 None:intergenic 45.0%
GGAGTGGTCGACTGTTAATT+TGG - chr6.4:52262169-52262188 MS.gene05191:intron 45.0%
TACCCAAGTTCACACAATCC+TGG - chr6.4:52262835-52262854 MS.gene05191:CDS 45.0%
TTGTGGCCAAAGCAACGTTT+AGG + chr6.4:52262662-52262681 None:intergenic 45.0%
! CTGTCATGTGCTGTTATCTG+TGG - chr6.4:52262109-52262128 MS.gene05191:intron 45.0%
! GTGTTTTGTGGGGACAGTTA+GGG - chr6.4:52262147-52262166 MS.gene05191:intron 45.0%
! GTTGCTTTGGCCACAATTCT+TGG - chr6.4:52262666-52262685 MS.gene05191:intron 45.0%
! TGTGTTTTGTGGGGACAGTT+AGG - chr6.4:52262146-52262165 MS.gene05191:intron 45.0%
! TGTTTTGTGGGGACAGTTAG+GGG - chr6.4:52262148-52262167 MS.gene05191:intron 45.0%
!! CTGCTGCTGTTTGTGTTTTG+TGG - chr6.4:52262135-52262154 MS.gene05191:intron 45.0%
!! GCTGCTGTTTGTGTTTTGTG+GGG - chr6.4:52262137-52262156 MS.gene05191:intron 45.0%
!! GTCGACTGTTAATTTGGTGC+TGG - chr6.4:52262175-52262194 MS.gene05191:intron 45.0%
AAAAACGCAGACAGCAGCAC+AGG + chr6.4:52262047-52262066 None:intergenic 50.0%
CAACAGCTACGACATCGATC+GGG - chr6.4:52260897-52260916 MS.gene05191:intron 50.0%
CCAACAGCTACGACATCGAT+CGG - chr6.4:52260896-52260915 MS.gene05191:intron 50.0%
CTGTTGTGAGCTGTTTGTGC+AGG - chr6.4:52262021-52262040 MS.gene05191:intron 50.0%
GAAGTTCGGAGACAACAGAG+CGG + chr6.4:52260966-52260985 None:intergenic 50.0%
GCACAAACAGCTCACAACAG+CGG + chr6.4:52262021-52262040 None:intergenic 50.0%
GCACACAACACAACCTAGCT+TGG - chr6.4:52261660-52261679 MS.gene05191:intron 50.0%
TGTCCAAAAACAGCCTCTGC+AGG + chr6.4:52262075-52262094 None:intergenic 50.0%
TTGGAGAAGAATTTGCGCGG+TGG + chr6.4:52260880-52260899 None:intergenic 50.0%
! CTCGACGAGGAGATGTTTTG+AGG - chr6.4:52261986-52262005 MS.gene05191:intron 50.0%
!!! GTGCTGCTGTCTGCGTTTTT+TGG - chr6.4:52262047-52262066 MS.gene05191:intron 50.0%
CCGATCGATGTCGTAGCTGT+TGG + chr6.4:52260899-52260918 None:intergenic 55.0%
GGTGATGAAGACGAGGAGGA+CGG + chr6.4:52260859-52260878 None:intergenic 55.0%
!! GCACCTGCAGAGGCTGTTTT+TGG - chr6.4:52262069-52262088 MS.gene05191:intron 55.0%
!!! CGAGGAGATGTTTTGAGGCG+TGG - chr6.4:52261991-52262010 MS.gene05191:intron 55.0%
!!! GCGTTTTTTGGCACCTGCAG+AGG - chr6.4:52262059-52262078 MS.gene05191:intron 55.0%
AAGGCGGCGATGGTTGGCAT+GGG + chr6.4:52260997-52261016 None:intergenic 60.0%
ACAAGGTTGGGTGCTCGACG+AGG - chr6.4:52261973-52261992 MS.gene05191:intron 60.0%
AGCGGAGGCGATAGTCGATG+AGG + chr6.4:52260948-52260967 None:intergenic 60.0%
GAGGATGTAACCTGCGGCGA+AGG + chr6.4:52261030-52261049 None:intergenic 60.0%
GTTCGGAGACAACAGAGCGG+AGG + chr6.4:52260963-52260982 None:intergenic 60.0%
TGTGGGGACAGTTAGGGGAG+TGG - chr6.4:52262153-52262172 MS.gene05191:intron 60.0%
! CGGTGGTGATGAAGACGAGG+AGG + chr6.4:52260863-52260882 None:intergenic 60.0%
ACGGTGAAGGCGGCGATGGT+TGG + chr6.4:52261003-52261022 None:intergenic 65.0%
GAAGGCGGCGATGGTTGGCA+TGG + chr6.4:52260998-52261017 None:intergenic 65.0%
! AACCTGCGGCGAAGGCGTGA+CGG + chr6.4:52261022-52261041 None:intergenic 65.0%
! CGAAGGCGTGACGGTGAAGG+CGG + chr6.4:52261013-52261032 None:intergenic 65.0%
! GCGCGGTGGTGATGAAGACG+AGG + chr6.4:52260866-52260885 None:intergenic 65.0%
CGTGACGGTGAAGGCGGCGA+TGG + chr6.4:52261007-52261026 None:intergenic 70.0%
! CGGCGAAGGCGTGACGGTGA+AGG + chr6.4:52261016-52261035 None:intergenic 70.0%
CACCGTCACGCCTTCGCCGC+AGG - chr6.4:52261017-52261036 MS.gene05191:intron 75.0%


Chromosome Type Strat End Strand Name
chr6.4 gene 52260814 52262902 52260814 ID=MS.gene05191
chr6.4 mRNA 52260814 52262902 52260814 ID=MS.gene05191.t1;Parent=MS.gene05191
chr6.4 exon 52262678 52262902 52262678 ID=MS.gene05191.t1.exon1;Parent=MS.gene05191.t1
chr6.4 CDS 52262678 52262902 52262678 ID=cds.MS.gene05191.t1;Parent=MS.gene05191.t1
chr6.4 exon 52260814 52260903 52260814 ID=MS.gene05191.t1.exon2;Parent=MS.gene05191.t1
chr6.4 CDS 52260814 52260903 52260814 ID=cds.MS.gene05191.t1;Parent=MS.gene05191.t1
Gene Sequence

>MS.gene05191

ATGAACACTGTCTCAACCCCACACTTCTCAATTCAATCAAATCCGTCCTCCTCGTCTTCATCACCACCGCGCAAATTCTTCTCCAACAGCTACGACATCGATCGGGCACAAAACAACAGCTCTCGCATGATCCTCATCGACTATCGCCTCCGCTCTGTTGTCTCCGAACTTCTCTCTCATCCCATGCCAACCATCGCCGCCTTCACCGTCACGCCTTCGCCGCAGGTTACATCCTCGCATATCTTTTCTTTATGTTGTCCATATCTGTAACTTTTTCATGTTAGTATCTATAAACCTTTTGAATTCATGATTTCTTGGACTGCTACATGTATATTTTTCACATGTATTCTAACTGTTTGATGAAAAGCCCAACTGAAAAAAAAATACAAAAAACCATTCTCTTTCCAATTTTTCAGAGTTAGGATTTGAAGATGGTTTATTTTTTATTCGATTTGTTGAACTTAAGTTTGTTGAATTTAACGCAGGGCCTTTTTGGATTGACATATATAATATTCTATGTGATTCTGTGTGATTTTTTTAGAAGGAAATTATGTTTCTTAATATATAAACAGACATGTTTTTGGTTTGTTTACTTGAAAACCTGGAGAAAATACTTTAACTTGAAATTTTGTATTTCTCTTATTGTGTTTTTTGGACATTTGATTGGTAAGTGAATTGATATGTTGATCAAGATCTCCAAAGAAGTGTTGTTCTGTTATATTGATTGGAAGCCATTGATTTTGGCTGAAGAATGATTGTTGAGAAAGAAATAGATAAGACTGAAGGTGCACCACAACCAAATGCAGTTTTTTATGATTGTTTGAAGCCTTGAACTTTCATATATACGCACACAACACAACCTAGCTTGGTATTAAAATGAGTTTTTGATGAGAGTTGTAATGCATTATAGGAATCCAACTATAGTAATGCAATTTGTTATATCAAACTTGCTTTCTCCATATTTAACTGAAGATAATTGATGAATCCACAAATGACTAATGTTGCCTTATCTGTTTTGGTTCACTAATTCTCTGGTTTTGTTTTGGTATTTAATAGCCAATGGAAAGATTTGTTTCTTTTGATCAAATTTAGGTAGATTTTTGTTGTTGTCATTGTGTTTGATTTGTATGGTATTTAAATTTCTACTTCAATTTACAATACAAGGTTGGGTGCTCGACGAGGAGATGTTTTGAGGCGTGGTGTGCCGCTGTTGTGAGCTGTTTGTGCAGGCCTGTGCTGCTGTCTGCGTTTTTTGGCACCTGCAGAGGCTGTTTTTGGACAATCACGAGCTGCTGCTGTCATGTGCTGTTATCTGTGGGTGCTGCTGCTGTTTGTGTTTTGTGGGGACAGTTAGGGGAGTGGTCGACTGTTAATTTGGTGCTGGTTAAAGTTCATATAAAAGTTTGCAGGTAAAAAGTTCTAAGAATGGACGTTGATTGACTTCTAAAAAATATGATGAAGAAAATTTATTTTAGTTTCAAATTATTATGTTGTTGAGGAAGCTTATCACTCTTCCAAAGCTTATTACTCTCTGTTCCTCATTTTGTCATTGATGTTGTCTTAACAATTTTATATTCAGTTTCATTCTCTTTCTATTTGCTTTTTACTGAAGTGTCTCCAATTTCATGTAGAAAAAACTGATGTGGAGAACATGATAATCGAATGAGTAGCATATATTTGTTTGAATTTCAATTTATTATACGTCAAATATCCATGCCAATATGTTTAAGTTTGTGCTTTAATTCTTTTTTCTTTTATATTCTGTGGTTCATGAATCAAACTGGTGAAATATCAAAGCGATAGTACACAAATCCTTCAATTGAGTTCTTTTCAAGAGTTATTTTTCCTAAACGTTGCTTTGGCCACAATTCTTGGTATATCAATTTGAAGCTTCTGTGTTGTTGAATACATCATATATATAATTTAGATCAATATAATCAATCTATATAATCAATATAATCAATATAATTTGCAGTTTTCTGATTATTTTTTGTTGTAGGCATTTCGGATATGTACCTATTTACCCAAGTTCACACAATCCTGGAATATTGAAGTAACATACTACATGAGTTACGGCAAAAAAAATTAG

Protein sequence

>MS.gene05191.t1

MNTVSTPHFSIQSNPSSSSSSPPRKFFSNSYDIDRAQNNSSRMILIDYRLRSVVSELLSHPMPTIAAFTVTPSPQAFRICTYLPKFTQSWNIEVTYYMSYGKKN