Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65108.t1 | XP_003622954.1 | 90.5 | 179 | 17 | 0 | 5 | 183 | 40 | 218 | 9.70E-81 | 309.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65108.t1 | G7L1B6 | 90.5 | 179 | 17 | 0 | 5 | 183 | 40 | 218 | 7.0e-81 | 309.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049758 | MS.gene65108 | 0.805892 | 1.07E-49 | -1.69E-46 |
| MS.gene050301 | MS.gene65108 | 0.823718 | 1.19E-53 | -1.69E-46 |
| MS.gene050558 | MS.gene65108 | 0.801877 | 7.32E-49 | -1.69E-46 |
| MS.gene05101 | MS.gene65108 | 0.810035 | 1.41E-50 | -1.69E-46 |
| MS.gene051662 | MS.gene65108 | 0.801763 | 7.72E-49 | -1.69E-46 |
| MS.gene051663 | MS.gene65108 | 0.844386 | 7.81E-59 | -1.69E-46 |
| MS.gene053836 | MS.gene65108 | 0.845814 | 3.21E-59 | -1.69E-46 |
| MS.gene05455 | MS.gene65108 | 0.822899 | 1.85E-53 | -1.69E-46 |
| MS.gene055390 | MS.gene65108 | 0.802221 | 6.22E-49 | -1.69E-46 |
| MS.gene055422 | MS.gene65108 | 0.812307 | 4.53E-51 | -1.69E-46 |
| MS.gene055428 | MS.gene65108 | 0.825396 | 4.79E-54 | -1.69E-46 |
| MS.gene055712 | MS.gene65108 | 0.810085 | 1.37E-50 | -1.69E-46 |
| MS.gene056407 | MS.gene65108 | 0.812166 | 4.86E-51 | -1.69E-46 |
| MS.gene056875 | MS.gene65108 | 0.809767 | 1.61E-50 | -1.69E-46 |
| MS.gene057448 | MS.gene65108 | 0.806384 | 8.45E-50 | -1.69E-46 |
| MS.gene057450 | MS.gene65108 | 0.814966 | 1.18E-51 | -1.69E-46 |
| MS.gene058002 | MS.gene65108 | 0.831091 | 2.03E-55 | -1.69E-46 |
| MS.gene05811 | MS.gene65108 | 0.832442 | 9.43E-56 | -1.69E-46 |
| MS.gene05907 | MS.gene65108 | 0.849594 | 2.92E-60 | -1.69E-46 |
| MS.gene059597 | MS.gene65108 | 0.808321 | 3.28E-50 | -1.69E-46 |
| MS.gene059676 | MS.gene65108 | 0.810496 | 1.12E-50 | -1.69E-46 |
| MS.gene059894 | MS.gene65108 | 0.805668 | 1.19E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65108.t1 | MTR_7g058760 | 90.503 | 179 | 17 | 0 | 5 | 183 | 40 | 218 | 3.11e-111 | 327 |
| MS.gene65108.t1 | MTR_7g058760 | 88.827 | 179 | 17 | 1 | 5 | 183 | 40 | 215 | 5.43e-107 | 316 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65108.t1 | AT5G48830 | 39.437 | 142 | 82 | 1 | 44 | 181 | 72 | 213 | 2.28e-22 | 94.0 |
| MS.gene65108.t1 | AT5G48830 | 39.437 | 142 | 82 | 1 | 44 | 181 | 72 | 213 | 2.73e-22 | 94.0 |
Find 37 sgRNAs with CRISPR-Local
Find 64 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAATTCAAGCCGAGTGTATT+TGG | 0.164191 | 7.1:+42815014 | MS.gene65108:CDS |
| CGTGACAGCTCAGACATTTC+TGG | 0.224603 | 7.1:+42815263 | MS.gene65108:CDS |
| CCTATGCCAATGCAATTTAA+AGG | 0.247757 | 7.1:-42815077 | None:intergenic |
| AATTCAAGCCGAGTGTATTT+GGG | 0.298989 | 7.1:+42815015 | MS.gene65108:CDS |
| AAGTATATTGCTCACTTATA+TGG | 0.317130 | 7.1:+42815172 | MS.gene65108:CDS |
| TCCAAAGTTAAGGTTACTTT+GGG | 0.362833 | 7.1:+42815546 | MS.gene65108:CDS |
| GTCCAAAGTTAAGGTTACTT+TGG | 0.374599 | 7.1:+42815545 | MS.gene65108:CDS |
| TTGCATTGGCATAGGAGCTT+TGG | 0.387344 | 7.1:+42815085 | MS.gene65108:CDS |
| TCCACTGGTTGTACAGAAAC+AGG | 0.392162 | 7.1:-42814863 | None:intergenic |
| TCTTTGTTGCTTCTATTGCC+TGG | 0.403195 | 7.1:+42814887 | MS.gene65108:CDS |
| TTGCCGAGGGTCCAAAGTTA+AGG | 0.405993 | 7.1:+42815536 | MS.gene65108:CDS |
| TCTAAATGCATTAGAGAAAG+AGG | 0.418471 | 7.1:+42815456 | MS.gene65108:CDS |
| CGGGTATAAGATTTGTCAAC+AGG | 0.422732 | 7.1:-42815206 | None:intergenic |
| CTATGCCAATGCAATTTAAA+GGG | 0.428572 | 7.1:-42815076 | None:intergenic |
| TGGTGGAACAGCATCTAATT+TGG | 0.431361 | 7.1:+42815118 | MS.gene65108:CDS |
| TTGCAGTGCGGTGCAGCAAA+AGG | 0.455706 | 7.1:+42815378 | MS.gene65108:intron |
| GCCCAAAGTAACCTTAACTT+TGG | 0.458602 | 7.1:-42815547 | None:intergenic |
| AAAAGAAACTGATTAACAAC+TGG | 0.461741 | 7.1:-42815143 | None:intergenic |
| GCATTCCCTTTAAATTGCAT+TGG | 0.484750 | 7.1:+42815071 | MS.gene65108:CDS |
| ATATTGCTCACTTATATGGC+TGG | 0.502407 | 7.1:+42815176 | MS.gene65108:CDS |
| GGAGCTTTGGTTGACTCTGA+TGG | 0.508159 | 7.1:+42815098 | MS.gene65108:CDS |
| CCTTTAAATTGCATTGGCAT+AGG | 0.508640 | 7.1:+42815077 | MS.gene65108:CDS |
| TAACCTTAACTTTGGACCCT+CGG | 0.519078 | 7.1:-42815539 | None:intergenic |
| TTAGAGAAAGAGGCTTATGC+GGG | 0.522119 | 7.1:+42815466 | MS.gene65108:CDS |
| TCCTGTTTCTGTACAACCAG+TGG | 0.523707 | 7.1:+42814862 | MS.gene65108:CDS |
| AGTTTAAAAGCTGTTGCCGA+GGG | 0.560847 | 7.1:+42815523 | MS.gene65108:CDS |
| ATTCAAGCCGAGTGTATTTG+GGG | 0.587572 | 7.1:+42815016 | MS.gene65108:CDS |
| TTAAAGGGAATGCCCGAAGA+CGG | 0.596553 | 7.1:-42815061 | None:intergenic |
| AGAAGCAACAAAGATTCCAC+TGG | 0.596892 | 7.1:-42814878 | None:intergenic |
| ATTAGAGAAAGAGGCTTATG+CGG | 0.602745 | 7.1:+42815465 | MS.gene65108:CDS |
| GAGTTTAAAAGCTGTTGCCG+AGG | 0.616763 | 7.1:+42815522 | MS.gene65108:CDS |
| GCTTTGGTTGACTCTGATGG+TGG | 0.626948 | 7.1:+42815101 | MS.gene65108:CDS |
| TCTGCAGCCCCAAATACACT+CGG | 0.654793 | 7.1:-42815023 | None:intergenic |
| GTCAAATTATGTATTAGATG+TGG | 0.662552 | 7.1:+42815411 | MS.gene65108:CDS |
| ACAGCTCAGACATTTCTGGT+AGG | 0.677195 | 7.1:+42815267 | MS.gene65108:CDS |
| TAGAGAAAGAGGCTTATGCG+GGG | 0.683596 | 7.1:+42815467 | MS.gene65108:CDS |
| AGAGAAAGAGGCTTATGCGG+GGG | 0.688620 | 7.1:+42815468 | MS.gene65108:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTTATTTTTGACATATGA+TGG | + | chr7.1:42815320-42815339 | MS.gene65108:intron | 15.0% |
| !!! | TATGTTATTTTGGTATGATT+TGG | + | chr7.1:42815642-42815661 | MS.gene65108:intron | 20.0% |
| ! | AAAAGAAACTGATTAACAAC+TGG | - | chr7.1:42815146-42815165 | None:intergenic | 25.0% |
| ! | AAGTATATTGCTCACTTATA+TGG | + | chr7.1:42815172-42815191 | MS.gene65108:CDS | 25.0% |
| ! | AGCAAAAGGAAAATATAGAA+AGG | - | chr7.1:42814941-42814960 | None:intergenic | 25.0% |
| ! | GTCAAATTATGTATTAGATG+TGG | + | chr7.1:42815411-42815430 | MS.gene65108:CDS | 25.0% |
| ! | TAAATGTTTCAAATGACTTC+TGG | + | chr7.1:42815695-42815714 | MS.gene65108:intron | 25.0% |
| !! | GTTAATCAGTTTCTTTTGAT+GGG | + | chr7.1:42815149-42815168 | MS.gene65108:CDS | 25.0% |
| !! | TGTTAATCAGTTTCTTTTGA+TGG | + | chr7.1:42815148-42815167 | MS.gene65108:CDS | 25.0% |
| !!! | AATTTTGACAAGTTGTAACT+TGG | + | chr7.1:42815665-42815684 | MS.gene65108:intron | 25.0% |
| CTATGCCAATGCAATTTAAA+GGG | - | chr7.1:42815079-42815098 | None:intergenic | 30.0% | |
| TAAAAATTACTCACCGTCTT+CGG | + | chr7.1:42815048-42815067 | MS.gene65108:CDS | 30.0% | |
| TCTAAATGCATTAGAGAAAG+AGG | + | chr7.1:42815456-42815475 | MS.gene65108:CDS | 30.0% | |
| ! | AGAATTGTTCTTTTGATCAC+GGG | - | chr7.1:42815228-42815247 | None:intergenic | 30.0% |
| ! | CAGAATTGTTCTTTTGATCA+CGG | - | chr7.1:42815229-42815248 | None:intergenic | 30.0% |
| ! | TCCAAAGTTAAGGTTACTTT+GGG | + | chr7.1:42815546-42815565 | MS.gene65108:CDS | 30.0% |
| !! | CTGCACATGTTATGTTATTT+TGG | + | chr7.1:42815632-42815651 | MS.gene65108:intron | 30.0% |
| !!! | GCTTGAATTTTTTCTGTGTA+AGG | - | chr7.1:42815004-42815023 | None:intergenic | 30.0% |
| AAAAATTACTCACCGTCTTC+GGG | + | chr7.1:42815049-42815068 | MS.gene65108:CDS | 35.0% | |
| AAATTCAAGCCGAGTGTATT+TGG | + | chr7.1:42815014-42815033 | MS.gene65108:CDS | 35.0% | |
| AATTCAAGCCGAGTGTATTT+GGG | + | chr7.1:42815015-42815034 | MS.gene65108:CDS | 35.0% | |
| ACAACCAAATTCATGATACC+AGG | - | chr7.1:42814908-42814927 | None:intergenic | 35.0% | |
| ATATTGCTCACTTATATGGC+TGG | + | chr7.1:42815176-42815195 | MS.gene65108:CDS | 35.0% | |
| ATTAGAGAAAGAGGCTTATG+CGG | + | chr7.1:42815465-42815484 | MS.gene65108:CDS | 35.0% | |
| ATTGCCTGGTATCATGAATT+TGG | + | chr7.1:42814901-42814920 | MS.gene65108:intron | 35.0% | |
| CCTATGCCAATGCAATTTAA+AGG | - | chr7.1:42815080-42815099 | None:intergenic | 35.0% | |
| GAAATACTTTCTGCAGCAAA+AGG | - | chr7.1:42814955-42814974 | None:intergenic | 35.0% | |
| GCATTCCCTTTAAATTGCAT+TGG | + | chr7.1:42815071-42815090 | MS.gene65108:CDS | 35.0% | |
| GTAAGGGTAAGAAAGTCAAA+TGG | + | chr7.1:42815289-42815308 | MS.gene65108:intron | 35.0% | |
| GTCCAAAGTTAAGGTTACTT+TGG | + | chr7.1:42815545-42815564 | MS.gene65108:CDS | 35.0% | |
| ! | CCTTTAAATTGCATTGGCAT+AGG | + | chr7.1:42815077-42815096 | MS.gene65108:CDS | 35.0% |
| ! | GCAACAGCTTTTAAACTCAA+GGG | - | chr7.1:42815520-42815539 | None:intergenic | 35.0% |
| AGAAGCAACAAAGATTCCAC+TGG | - | chr7.1:42814881-42814900 | None:intergenic | 40.0% | |
| AGTTTAAAAGCTGTTGCCGA+GGG | + | chr7.1:42815523-42815542 | MS.gene65108:CDS | 40.0% | |
| ATTCAAGCCGAGTGTATTTG+GGG | + | chr7.1:42815016-42815035 | MS.gene65108:CDS | 40.0% | |
| CGGGTATAAGATTTGTCAAC+AGG | - | chr7.1:42815209-42815228 | None:intergenic | 40.0% | |
| GCCCAAAGTAACCTTAACTT+TGG | - | chr7.1:42815550-42815569 | None:intergenic | 40.0% | |
| TAACCTTAACTTTGGACCCT+CGG | - | chr7.1:42815542-42815561 | None:intergenic | 40.0% | |
| TGGTGGAACAGCATCTAATT+TGG | + | chr7.1:42815118-42815137 | MS.gene65108:CDS | 40.0% | |
| TTAGAGAAAGAGGCTTATGC+GGG | + | chr7.1:42815466-42815485 | MS.gene65108:CDS | 40.0% | |
| ! | AGGGTCTTTTTGCAGATTGA+AGG | - | chr7.1:42815501-42815520 | None:intergenic | 40.0% |
| ! | CTTTTGTCTCTTTTGCAGTG+CGG | + | chr7.1:42815366-42815385 | MS.gene65108:intron | 40.0% |
| ! | GGCAACAGCTTTTAAACTCA+AGG | - | chr7.1:42815521-42815540 | None:intergenic | 40.0% |
| ! | TCTTTGTTGCTTCTATTGCC+TGG | + | chr7.1:42814887-42814906 | MS.gene65108:CDS | 40.0% |
| !! | CAGACATTTCTGGTAGGTAA+GGG | + | chr7.1:42815273-42815292 | MS.gene65108:intron | 40.0% |
| !! | TCAGACATTTCTGGTAGGTA+AGG | + | chr7.1:42815272-42815291 | MS.gene65108:intron | 40.0% |
| !! | TTTTCTGCAAGTTGAGGAAG+AGG | + | chr7.1:42815573-42815592 | MS.gene65108:CDS | 40.0% |
| GAGTTTAAAAGCTGTTGCCG+AGG | + | chr7.1:42815522-42815541 | MS.gene65108:CDS | 45.0% | |
| TAGAGAAAGAGGCTTATGCG+GGG | + | chr7.1:42815467-42815486 | MS.gene65108:CDS | 45.0% | |
| TTAAAGGGAATGCCCGAAGA+CGG | - | chr7.1:42815064-42815083 | None:intergenic | 45.0% | |
| ! | TCCACTGGTTGTACAGAAAC+AGG | - | chr7.1:42814866-42814885 | None:intergenic | 45.0% |
| ! | TGCAAGTTGAGGAAGAGGTA+GGG | + | chr7.1:42815578-42815597 | MS.gene65108:intron | 45.0% |
| ! | TTGCATTGGCATAGGAGCTT+TGG | + | chr7.1:42815085-42815104 | MS.gene65108:CDS | 45.0% |
| !! | ACAGCTCAGACATTTCTGGT+AGG | + | chr7.1:42815267-42815286 | MS.gene65108:CDS | 45.0% |
| !! | TCCTGTTTCTGTACAACCAG+TGG | + | chr7.1:42814862-42814881 | MS.gene65108:CDS | 45.0% |
| AGAGAAAGAGGCTTATGCGG+GGG | + | chr7.1:42815468-42815487 | MS.gene65108:CDS | 50.0% | |
| CGTGACAGCTCAGACATTTC+TGG | + | chr7.1:42815263-42815282 | MS.gene65108:CDS | 50.0% | |
| TCTGCAGCCCCAAATACACT+CGG | - | chr7.1:42815026-42815045 | None:intergenic | 50.0% | |
| TTGCCGAGGGTCCAAAGTTA+AGG | + | chr7.1:42815536-42815555 | MS.gene65108:CDS | 50.0% | |
| ! | CTGCAAGTTGAGGAAGAGGT+AGG | + | chr7.1:42815577-42815596 | MS.gene65108:intron | 50.0% |
| ! | GCTTTGGTTGACTCTGATGG+TGG | + | chr7.1:42815101-42815120 | MS.gene65108:CDS | 50.0% |
| ! | GGAGCTTTGGTTGACTCTGA+TGG | + | chr7.1:42815098-42815117 | MS.gene65108:CDS | 50.0% |
| !!! | GGCTGCTTTTCTGCAAGTTG+AGG | + | chr7.1:42815567-42815586 | MS.gene65108:CDS | 50.0% |
| TTGCAGTGCGGTGCAGCAAA+AGG | + | chr7.1:42815378-42815397 | MS.gene65108:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 42814855 | 42815774 | 42814855 | ID=MS.gene65108 |
| chr7.1 | mRNA | 42814855 | 42815774 | 42814855 | ID=MS.gene65108.t1;Parent=MS.gene65108 |
| chr7.1 | exon | 42814855 | 42814908 | 42814855 | ID=MS.gene65108.t1.exon1;Parent=MS.gene65108.t1 |
| chr7.1 | CDS | 42814855 | 42814908 | 42814855 | ID=cds.MS.gene65108.t1;Parent=MS.gene65108.t1 |
| chr7.1 | exon | 42815011 | 42815288 | 42815011 | ID=MS.gene65108.t1.exon2;Parent=MS.gene65108.t1 |
| chr7.1 | CDS | 42815011 | 42815288 | 42815011 | ID=cds.MS.gene65108.t1;Parent=MS.gene65108.t1 |
| chr7.1 | exon | 42815384 | 42815594 | 42815384 | ID=MS.gene65108.t1.exon3;Parent=MS.gene65108.t1 |
| chr7.1 | CDS | 42815384 | 42815594 | 42815384 | ID=cds.MS.gene65108.t1;Parent=MS.gene65108.t1 |
| chr7.1 | exon | 42815733 | 42815774 | 42815733 | ID=MS.gene65108.t1.exon4;Parent=MS.gene65108.t1 |
| chr7.1 | CDS | 42815733 | 42815774 | 42815733 | ID=cds.MS.gene65108.t1;Parent=MS.gene65108.t1 |
| Gene Sequence |
| Protein sequence |
