Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65365.t1 | RHN54425.1 | 96 | 350 | 12 | 1 | 1 | 350 | 206 | 553 | 7.30E-196 | 693 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65365.t1 | F4HUK6 | 48.2 | 355 | 171 | 5 | 2 | 350 | 183 | 530 | 9.3e-91 | 335.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65365.t1 | G7K007 | 96.0 | 350 | 12 | 1 | 1 | 350 | 181 | 528 | 5.3e-196 | 693.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050148 | MS.gene65365 | 0.803658 | 3.14E-49 | -1.69E-46 |
| MS.gene050961 | MS.gene65365 | 0.830156 | 3.44E-55 | -1.69E-46 |
| MS.gene05156 | MS.gene65365 | 0.818365 | 2.04E-52 | -1.69E-46 |
| MS.gene052053 | MS.gene65365 | 0.812496 | 4.12E-51 | -1.69E-46 |
| MS.gene052559 | MS.gene65365 | 0.839748 | 1.32E-57 | -1.69E-46 |
| MS.gene052560 | MS.gene65365 | 0.812534 | 4.04E-51 | -1.69E-46 |
| MS.gene053601 | MS.gene65365 | 0.806445 | 8.20E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65365.t1 | MTR_5g023730 | 96.000 | 350 | 12 | 1 | 1 | 350 | 181 | 528 | 0.0 | 692 |
| MS.gene65365.t1 | MTR_5g008800 | 49.435 | 354 | 168 | 5 | 2 | 350 | 177 | 524 | 1.13e-112 | 338 |
| MS.gene65365.t1 | MTR_5g008820 | 47.875 | 353 | 173 | 4 | 2 | 350 | 177 | 522 | 2.55e-112 | 337 |
| MS.gene65365.t1 | MTR_8g074040 | 47.887 | 355 | 172 | 5 | 2 | 350 | 103 | 450 | 1.46e-110 | 331 |
| MS.gene65365.t1 | MTR_8g074120 | 48.442 | 353 | 173 | 4 | 2 | 350 | 132 | 479 | 8.96e-110 | 330 |
| MS.gene65365.t1 | MTR_8g074040 | 47.887 | 355 | 172 | 5 | 2 | 350 | 221 | 568 | 3.96e-109 | 331 |
| MS.gene65365.t1 | MTR_4g121600 | 44.290 | 359 | 185 | 5 | 2 | 350 | 190 | 543 | 5.28e-98 | 301 |
| MS.gene65365.t1 | MTR_2g044180 | 43.454 | 359 | 188 | 5 | 2 | 350 | 99 | 452 | 4.18e-97 | 296 |
| MS.gene65365.t1 | MTR_2g044180 | 43.454 | 359 | 188 | 5 | 2 | 350 | 190 | 543 | 7.03e-96 | 296 |
| MS.gene65365.t1 | MTR_3g064420 | 43.258 | 356 | 185 | 6 | 3 | 346 | 184 | 534 | 2.45e-89 | 279 |
| MS.gene65365.t1 | MTR_5g076240 | 41.292 | 356 | 196 | 6 | 2 | 350 | 183 | 532 | 2.22e-86 | 271 |
| MS.gene65365.t1 | MTR_5g076220 | 42.297 | 357 | 192 | 7 | 2 | 350 | 183 | 533 | 1.35e-81 | 259 |
| MS.gene65365.t1 | MTR_0161s0050 | 41.737 | 357 | 194 | 7 | 2 | 350 | 185 | 535 | 1.26e-79 | 254 |
| MS.gene65365.t1 | MTR_5g076200 | 40.336 | 357 | 199 | 7 | 2 | 350 | 183 | 533 | 2.11e-77 | 248 |
| MS.gene65365.t1 | MTR_0803s0020 | 27.168 | 346 | 230 | 9 | 13 | 350 | 399 | 730 | 2.51e-27 | 113 |
| MS.gene65365.t1 | MTR_8g039720 | 27.811 | 338 | 219 | 11 | 4 | 331 | 205 | 527 | 1.82e-24 | 104 |
| MS.gene65365.t1 | MTR_2g095510 | 27.954 | 347 | 227 | 11 | 4 | 342 | 183 | 514 | 1.02e-23 | 102 |
| MS.gene65365.t1 | MTR_4g077900 | 26.479 | 355 | 224 | 11 | 7 | 344 | 121 | 455 | 1.10e-22 | 99.0 |
| MS.gene65365.t1 | MTR_1g026310 | 24.650 | 357 | 245 | 8 | 4 | 350 | 202 | 544 | 1.24e-22 | 99.4 |
| MS.gene65365.t1 | MTR_1g063110 | 26.437 | 348 | 235 | 9 | 4 | 342 | 188 | 523 | 1.34e-22 | 99.0 |
| MS.gene65365.t1 | MTR_1569s0010 | 27.954 | 347 | 226 | 9 | 13 | 350 | 188 | 519 | 1.56e-22 | 99.0 |
| MS.gene65365.t1 | MTR_2g105570 | 28.530 | 347 | 216 | 12 | 7 | 342 | 190 | 515 | 1.97e-22 | 98.6 |
| MS.gene65365.t1 | MTR_5g007640 | 26.902 | 368 | 222 | 12 | 1 | 339 | 220 | 569 | 2.33e-22 | 98.6 |
| MS.gene65365.t1 | MTR_4g057730 | 26.136 | 352 | 236 | 10 | 1 | 342 | 180 | 517 | 1.19e-21 | 96.3 |
| MS.gene65365.t1 | MTR_1g063120 | 25.429 | 350 | 236 | 11 | 4 | 342 | 192 | 527 | 2.32e-21 | 95.5 |
| MS.gene65365.t1 | MTR_4g005750 | 28.286 | 350 | 213 | 12 | 7 | 342 | 183 | 508 | 8.82e-21 | 93.6 |
| MS.gene65365.t1 | MTR_5g027500 | 26.503 | 366 | 229 | 14 | 4 | 350 | 208 | 552 | 1.42e-20 | 93.2 |
| MS.gene65365.t1 | MTR_3g460770 | 26.176 | 340 | 217 | 11 | 2 | 326 | 180 | 500 | 1.60e-20 | 93.2 |
| MS.gene65365.t1 | MTR_1g090737 | 26.111 | 360 | 228 | 11 | 1 | 344 | 206 | 543 | 1.99e-20 | 92.8 |
| MS.gene65365.t1 | MTR_5g027480 | 27.606 | 355 | 220 | 16 | 7 | 344 | 198 | 532 | 4.31e-20 | 91.7 |
| MS.gene65365.t1 | MTR_4g128337 | 25.364 | 343 | 223 | 11 | 4 | 331 | 188 | 512 | 3.34e-19 | 89.0 |
| MS.gene65365.t1 | MTR_4g057730 | 25.914 | 301 | 203 | 9 | 1 | 291 | 180 | 470 | 6.04e-18 | 85.1 |
| MS.gene65365.t1 | MTR_3g088880 | 23.446 | 354 | 235 | 11 | 4 | 342 | 199 | 531 | 7.44e-18 | 85.1 |
| MS.gene65365.t1 | MTR_3g035130 | 23.988 | 346 | 239 | 9 | 13 | 350 | 170 | 499 | 3.00e-17 | 83.2 |
| MS.gene65365.t1 | MTR_3g088870 | 22.689 | 357 | 237 | 11 | 4 | 342 | 198 | 533 | 9.13e-16 | 78.6 |
| MS.gene65365.t1 | MTR_1g073180 | 26.184 | 359 | 232 | 11 | 4 | 350 | 181 | 518 | 5.91e-15 | 76.3 |
| MS.gene65365.t1 | MTR_1586s0010 | 23.874 | 222 | 161 | 4 | 2 | 216 | 201 | 421 | 1.92e-14 | 74.3 |
| MS.gene65365.t1 | MTR_1g086600 | 33.793 | 145 | 86 | 4 | 149 | 292 | 438 | 573 | 4.55e-14 | 73.6 |
| MS.gene65365.t1 | MTR_7g109830 | 27.551 | 196 | 130 | 4 | 100 | 292 | 363 | 549 | 4.63e-14 | 73.6 |
| MS.gene65365.t1 | MTR_4g057730 | 25.352 | 284 | 192 | 9 | 1 | 274 | 180 | 453 | 5.95e-14 | 72.8 |
| MS.gene65365.t1 | MTR_5g009360 | 25.356 | 351 | 183 | 13 | 3 | 292 | 219 | 551 | 6.78e-14 | 73.2 |
| MS.gene65365.t1 | MTR_5g009360 | 25.356 | 351 | 183 | 13 | 3 | 292 | 93 | 425 | 8.16e-14 | 72.8 |
| MS.gene65365.t1 | MTR_1g073180 | 25.714 | 350 | 228 | 10 | 4 | 342 | 203 | 531 | 2.67e-13 | 71.2 |
| MS.gene65365.t1 | MTR_4g068670 | 26.638 | 229 | 129 | 7 | 71 | 292 | 352 | 548 | 3.55e-13 | 70.9 |
| MS.gene65365.t1 | MTR_5g009350 | 25.215 | 349 | 186 | 12 | 3 | 292 | 219 | 551 | 3.68e-13 | 70.9 |
| MS.gene65365.t1 | MTR_5g009350 | 25.215 | 349 | 186 | 12 | 3 | 292 | 219 | 551 | 3.85e-13 | 70.9 |
| MS.gene65365.t1 | MTR_1054s0020 | 25.753 | 299 | 189 | 8 | 10 | 300 | 433 | 706 | 7.35e-13 | 70.1 |
| MS.gene65365.t1 | MTR_2g030240 | 24.352 | 386 | 227 | 14 | 1 | 338 | 55 | 423 | 1.80e-12 | 68.6 |
| MS.gene65365.t1 | MTR_5g009350 | 25.072 | 347 | 185 | 12 | 3 | 290 | 219 | 549 | 1.92e-12 | 68.6 |
| MS.gene65365.t1 | MTR_2g030240 | 24.352 | 386 | 227 | 14 | 1 | 338 | 149 | 517 | 2.04e-12 | 68.6 |
| MS.gene65365.t1 | MTR_5g009350 | 25.146 | 342 | 181 | 12 | 3 | 285 | 219 | 544 | 2.52e-12 | 68.2 |
| MS.gene65365.t1 | MTR_1g034360 | 28.481 | 158 | 91 | 6 | 142 | 292 | 408 | 550 | 1.01e-11 | 66.6 |
| MS.gene65365.t1 | MTR_7g084850 | 25.485 | 361 | 221 | 13 | 7 | 340 | 358 | 697 | 1.21e-11 | 66.2 |
| MS.gene65365.t1 | MTR_8g099420 | 25.070 | 355 | 179 | 16 | 3 | 292 | 220 | 552 | 8.59e-11 | 63.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65365.t1 | AT1G20560 | 48.169 | 355 | 171 | 5 | 2 | 350 | 105 | 452 | 1.40e-112 | 336 |
| MS.gene65365.t1 | AT1G20560 | 48.169 | 355 | 171 | 5 | 2 | 350 | 183 | 530 | 1.06e-111 | 336 |
| MS.gene65365.t1 | AT2G17650 | 48.876 | 356 | 168 | 6 | 2 | 350 | 237 | 585 | 2.67e-108 | 329 |
| MS.gene65365.t1 | AT3G16910 | 43.213 | 361 | 186 | 6 | 2 | 350 | 193 | 546 | 1.50e-92 | 287 |
| MS.gene65365.t1 | AT1G76290 | 43.575 | 358 | 185 | 7 | 2 | 350 | 173 | 522 | 2.38e-88 | 276 |
| MS.gene65365.t1 | AT1G65890 | 40.443 | 361 | 195 | 6 | 3 | 348 | 183 | 538 | 1.19e-86 | 272 |
| MS.gene65365.t1 | AT1G66120 | 41.808 | 354 | 193 | 6 | 4 | 349 | 184 | 532 | 2.89e-86 | 271 |
| MS.gene65365.t1 | AT1G65880 | 40.055 | 362 | 196 | 6 | 3 | 348 | 183 | 539 | 6.54e-83 | 263 |
| MS.gene65365.t1 | AT1G75960 | 41.457 | 357 | 194 | 7 | 2 | 350 | 180 | 529 | 1.59e-82 | 261 |
| MS.gene65365.t1 | AT5G16340 | 40.169 | 356 | 199 | 6 | 2 | 350 | 181 | 529 | 1.90e-82 | 261 |
| MS.gene65365.t1 | AT5G16370 | 39.326 | 356 | 202 | 6 | 2 | 350 | 181 | 529 | 2.05e-81 | 258 |
| MS.gene65365.t1 | AT1G77240 | 39.437 | 355 | 203 | 7 | 2 | 350 | 181 | 529 | 2.36e-81 | 258 |
| MS.gene65365.t1 | AT1G21540 | 38.936 | 357 | 205 | 6 | 2 | 350 | 183 | 534 | 3.00e-81 | 258 |
| MS.gene65365.t1 | AT1G21530 | 38.997 | 359 | 204 | 7 | 2 | 350 | 180 | 533 | 7.49e-81 | 256 |
| MS.gene65365.t1 | AT1G21530 | 38.997 | 359 | 202 | 8 | 2 | 350 | 180 | 531 | 9.68e-79 | 251 |
| MS.gene65365.t1 | AT1G68270 | 34.819 | 359 | 188 | 8 | 3 | 348 | 183 | 508 | 1.73e-58 | 197 |
| MS.gene65365.t1 | AT5G63380 | 28.161 | 348 | 225 | 12 | 4 | 342 | 202 | 533 | 3.61e-27 | 112 |
| MS.gene65365.t1 | AT1G20500 | 28.571 | 350 | 220 | 12 | 4 | 342 | 195 | 525 | 8.89e-24 | 102 |
| MS.gene65365.t1 | AT3G21240 | 27.350 | 351 | 227 | 11 | 7 | 344 | 196 | 531 | 2.88e-22 | 98.2 |
| MS.gene65365.t1 | AT4G05160 | 28.329 | 353 | 224 | 11 | 4 | 345 | 188 | 522 | 3.43e-22 | 97.8 |
| MS.gene65365.t1 | AT1G20510 | 28.011 | 357 | 232 | 12 | 4 | 350 | 187 | 528 | 1.92e-21 | 95.9 |
| MS.gene65365.t1 | AT4G19010 | 25.278 | 360 | 235 | 14 | 7 | 350 | 205 | 546 | 2.13e-20 | 92.8 |
| MS.gene65365.t1 | AT3G21240 | 28.428 | 299 | 190 | 10 | 7 | 292 | 196 | 483 | 5.21e-20 | 91.3 |
| MS.gene65365.t1 | AT3G21240 | 28.428 | 299 | 190 | 10 | 7 | 292 | 196 | 483 | 5.21e-20 | 91.3 |
| MS.gene65365.t1 | AT1G51680 | 27.941 | 340 | 217 | 12 | 7 | 333 | 203 | 527 | 7.28e-20 | 90.9 |
| MS.gene65365.t1 | AT1G51680 | 28.107 | 338 | 215 | 12 | 7 | 331 | 203 | 525 | 8.27e-20 | 90.9 |
| MS.gene65365.t1 | AT3G21230 | 27.221 | 349 | 225 | 10 | 7 | 342 | 211 | 543 | 6.80e-19 | 88.2 |
| MS.gene65365.t1 | AT3G21230 | 27.219 | 338 | 217 | 10 | 7 | 331 | 211 | 532 | 9.38e-19 | 87.8 |
| MS.gene65365.t1 | AT1G65060 | 27.083 | 336 | 221 | 11 | 7 | 331 | 206 | 528 | 1.80e-18 | 87.0 |
| MS.gene65365.t1 | AT1G20510 | 29.252 | 294 | 188 | 10 | 7 | 291 | 190 | 472 | 1.83e-18 | 86.7 |
| MS.gene65365.t1 | AT1G51680 | 28.763 | 299 | 189 | 10 | 7 | 292 | 203 | 490 | 2.26e-18 | 86.7 |
| MS.gene65365.t1 | AT5G38120 | 26.163 | 344 | 220 | 9 | 13 | 344 | 206 | 527 | 4.88e-18 | 85.5 |
| MS.gene65365.t1 | AT1G62940 | 23.932 | 351 | 243 | 7 | 5 | 344 | 180 | 517 | 1.83e-17 | 84.0 |
| MS.gene65365.t1 | AT2G47240 | 26.331 | 338 | 182 | 12 | 10 | 292 | 165 | 490 | 1.97e-17 | 84.0 |
| MS.gene65365.t1 | AT2G47240 | 26.331 | 338 | 182 | 12 | 10 | 292 | 224 | 549 | 2.15e-17 | 84.0 |
| MS.gene65365.t1 | AT2G47240 | 26.331 | 338 | 182 | 12 | 10 | 292 | 224 | 549 | 2.15e-17 | 84.0 |
| MS.gene65365.t1 | AT2G47240 | 26.331 | 338 | 182 | 12 | 10 | 292 | 224 | 549 | 2.15e-17 | 84.0 |
| MS.gene65365.t1 | AT5G38120 | 26.284 | 331 | 210 | 9 | 13 | 331 | 206 | 514 | 5.12e-17 | 82.4 |
| MS.gene65365.t1 | AT1G20480 | 25.978 | 358 | 235 | 13 | 4 | 350 | 211 | 549 | 5.56e-17 | 82.4 |
| MS.gene65365.t1 | AT3G48990 | 26.087 | 368 | 191 | 14 | 13 | 344 | 169 | 491 | 2.67e-16 | 80.5 |
| MS.gene65365.t1 | AT5G38120 | 27.148 | 291 | 182 | 8 | 13 | 291 | 206 | 478 | 4.14e-16 | 79.7 |
| MS.gene65365.t1 | AT5G38120 | 27.148 | 291 | 182 | 8 | 13 | 291 | 206 | 478 | 4.14e-16 | 79.7 |
| MS.gene65365.t1 | AT3G21230 | 27.759 | 299 | 191 | 9 | 7 | 292 | 211 | 497 | 1.56e-15 | 77.8 |
| MS.gene65365.t1 | AT1G30520 | 25.000 | 332 | 207 | 10 | 7 | 314 | 173 | 486 | 3.27e-12 | 67.8 |
| MS.gene65365.t1 | AT1G30520 | 25.000 | 332 | 207 | 10 | 7 | 314 | 173 | 486 | 3.27e-12 | 67.8 |
| MS.gene65365.t1 | AT1G20510 | 27.148 | 291 | 185 | 11 | 4 | 278 | 187 | 466 | 3.42e-12 | 67.8 |
| MS.gene65365.t1 | AT4G11030 | 26.872 | 227 | 134 | 10 | 82 | 292 | 342 | 552 | 6.55e-11 | 63.9 |
| MS.gene65365.t1 | AT1G65060 | 26.471 | 272 | 180 | 10 | 7 | 267 | 206 | 468 | 7.90e-11 | 63.5 |
Find 60 sgRNAs with CRISPR-Local
Find 75 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGAACACTCTTCATTGTATT+TGG | 0.117189 | 5.1:+14389829 | None:intergenic |
| CTGAATTATGTTGATCAAAA+AGG | 0.196037 | 5.1:-14389892 | MS.gene65365:CDS |
| AAAAGATATTATTTATTCTA+AGG | 0.210086 | 5.1:-14389638 | MS.gene65365:CDS |
| TTCCACTTTCTTACAATAAC+TGG | 0.223827 | 5.1:+14389943 | None:intergenic |
| TGATTATAAACTGCTCCTTT+TGG | 0.243384 | 5.1:+14390354 | None:intergenic |
| TTGCAAACTGTGACATTATT+AGG | 0.258350 | 5.1:-14390089 | MS.gene65365:CDS |
| ATAGGTGAAATCATGTTTAA+AGG | 0.270805 | 5.1:-14389790 | MS.gene65365:CDS |
| CTTGGATTTGATGTTAATAT+TGG | 0.274603 | 5.1:-14389991 | MS.gene65365:CDS |
| ATAGGATCACATTCATTTCT+AGG | 0.276426 | 5.1:+14390411 | None:intergenic |
| TCACCAAACACCACACTCTT+AGG | 0.297868 | 5.1:+14389406 | None:intergenic |
| TAATAGTCGCGAGTGAATTA+AGG | 0.299417 | 5.1:+14390319 | None:intergenic |
| GTGTGGTGCACCTACCCTTC+TGG | 0.311889 | 5.1:-14390118 | MS.gene65365:CDS |
| AACTACACATCAGGCTCAAC+TGG | 0.349825 | 5.1:-14390381 | MS.gene65365:CDS |
| TGAGTTTGTGAGTTCATTAG+AGG | 0.375347 | 5.1:-14389614 | MS.gene65365:CDS |
| TAGGTGAAATCATGTTTAAA+GGG | 0.386467 | 5.1:-14389789 | MS.gene65365:CDS |
| TGAAGCAATTCATGTTCATA+AGG | 0.387735 | 5.1:-14390151 | MS.gene65365:CDS |
| TTTGATGTTAATATTGGTTA+TGG | 0.391504 | 5.1:-14389985 | MS.gene65365:CDS |
| AAAGGGAATACTTCAATGTC+AGG | 0.397558 | 5.1:-14389772 | MS.gene65365:CDS |
| ACCTTACCAGTTGAATTAAG+AGG | 0.398367 | 5.1:+14389379 | None:intergenic |
| TTTAAACATGATTTCACCTA+TGG | 0.398368 | 5.1:+14389792 | None:intergenic |
| AAGGTGAGTGTCACAGTTGC+TGG | 0.399927 | 5.1:-14390054 | MS.gene65365:CDS |
| TTCAAATTGAAAGAACCATT+AGG | 0.416142 | 5.1:+14389670 | None:intergenic |
| GATTTGCCTCTTAATTCAAC+TGG | 0.421617 | 5.1:-14389385 | None:intergenic |
| ATTTGGTTAATGCCTGCTCT+TGG | 0.425029 | 5.1:-14390222 | MS.gene65365:CDS |
| GCAATGGATGGTGTTTCATT+TGG | 0.447289 | 5.1:-14390239 | MS.gene65365:CDS |
| TGATGATGGTGACGAGTGTT+TGG | 0.449095 | 5.1:-14389530 | MS.gene65365:CDS |
| ATGAAGAGTGTTCCATATGA+TGG | 0.450784 | 5.1:-14389820 | MS.gene65365:CDS |
| CTTAACAAAGTGGCAGAGCT+TGG | 0.451298 | 5.1:-14390009 | MS.gene65365:CDS |
| TGGTTAATGCCTGCTCTTGG+TGG | 0.459143 | 5.1:-14390219 | MS.gene65365:CDS |
| CTAGGTGTTAGGTTACCTAA+TGG | 0.459759 | 5.1:-14389685 | MS.gene65365:CDS |
| TATGGTATGACTGAAACATT+AGG | 0.461838 | 5.1:-14389967 | MS.gene65365:CDS |
| GGACTAGGGACCTAGGTGTT+AGG | 0.465511 | 5.1:-14389696 | MS.gene65365:CDS |
| GTTCCTAAGAGTGTGGTGTT+TGG | 0.467445 | 5.1:-14389409 | MS.gene65365:CDS |
| GTCCAGTTATTGTAAGAAAG+TGG | 0.471102 | 5.1:-14389945 | MS.gene65365:CDS |
| AGATCAATTAGATGCTCATA+TGG | 0.476810 | 5.1:-14389431 | MS.gene65365:CDS |
| AAAGATATTATTTATTCTAA+GGG | 0.478681 | 5.1:-14389637 | MS.gene65365:CDS |
| AGGGGTTATTGACTTTATGA+TGG | 0.478800 | 5.1:-14389872 | MS.gene65365:CDS |
| GGGATGGTTGGTATAGGACT+AGG | 0.479682 | 5.1:-14389711 | MS.gene65365:CDS |
| ACAGCTACTTTCAAAACCAT+TGG | 0.487308 | 5.1:+14389568 | None:intergenic |
| GTTTGTGAGTTCATTAGAGG+TGG | 0.501370 | 5.1:-14389611 | MS.gene65365:CDS |
| GATGTGTAGTTCACTGATAT+AGG | 0.502126 | 5.1:+14390393 | None:intergenic |
| GCAATAATTTCCAGAAGGGT+AGG | 0.508940 | 5.1:+14390108 | None:intergenic |
| GGCTCAACTGGAATTCCAAA+AGG | 0.522211 | 5.1:-14390369 | MS.gene65365:CDS |
| GCAATTGTGAAATTGAAAGA+TGG | 0.524748 | 5.1:-14389493 | MS.gene65365:CDS |
| GTTGACATGTTTAGATGCAA+TGG | 0.528978 | 5.1:-14390255 | MS.gene65365:CDS |
| ATATCAGTGAACTACACATC+AGG | 0.529465 | 5.1:-14390390 | MS.gene65365:CDS |
| TTTCGATGTGAATCCTATGC+TGG | 0.531354 | 5.1:-14390292 | MS.gene65365:CDS |
| ATGTTTGTGCCACCAAGAGC+AGG | 0.540220 | 5.1:+14390210 | None:intergenic |
| ATTCAAAATTCTTAACAAAG+TGG | 0.541410 | 5.1:-14390019 | MS.gene65365:CDS |
| GTTTGCAATAATTTCCAGAA+GGG | 0.591302 | 5.1:+14390104 | None:intergenic |
| TTCAATTTCACAATTGCACA+AGG | 0.596592 | 5.1:+14389499 | None:intergenic |
| AAGGGAATACTTCAATGTCA+GGG | 0.598415 | 5.1:-14389771 | MS.gene65365:CDS |
| AGTTTGCAATAATTTCCAGA+AGG | 0.600004 | 5.1:+14390103 | None:intergenic |
| TGGTATAGGACTAGGGACCT+AGG | 0.607960 | 5.1:-14389703 | MS.gene65365:CDS |
| CATTAGGTAACCTAACACCT+AGG | 0.611239 | 5.1:+14389686 | None:intergenic |
| GTGGGGAGATATGATGATGA+TGG | 0.634328 | 5.1:-14389544 | MS.gene65365:CDS |
| GGATGGTTGGTATAGGACTA+GGG | 0.648496 | 5.1:-14389710 | MS.gene65365:CDS |
| ACATGTTTAGATGCAATGGA+TGG | 0.657007 | 5.1:-14390251 | MS.gene65365:CDS |
| CATAAGGTAACACATTTGTG+TGG | 0.685677 | 5.1:-14390135 | MS.gene65365:CDS |
| TCATATGGTTCCTAAGAGTG+TGG | 0.716052 | 5.1:-14389416 | MS.gene65365:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAGATATTATTTATTCTA+AGG | - | chr5.1:14390162-14390181 | MS.gene65365:CDS | 10.0% |
| !! | AAAGATATTATTTATTCTAA+GGG | - | chr5.1:14390163-14390182 | MS.gene65365:CDS | 10.0% |
| !! | ATTCAAAATTCTTAACAAAG+TGG | - | chr5.1:14389781-14389800 | MS.gene65365:CDS | 20.0% |
| !! | TGAATTATGTTGATCAAAAA+GGG | - | chr5.1:14389909-14389928 | MS.gene65365:CDS | 20.0% |
| !!! | TTGTTAAGAATTTTGAATGA+TGG | + | chr5.1:14389779-14389798 | None:intergenic | 20.0% |
| !!! | TTTGATGTTAATATTGGTTA+TGG | - | chr5.1:14389815-14389834 | MS.gene65365:CDS | 20.0% |
| ! | ATAGGTGAAATCATGTTTAA+AGG | - | chr5.1:14390010-14390029 | MS.gene65365:CDS | 25.0% |
| ! | CTGAATTATGTTGATCAAAA+AGG | - | chr5.1:14389908-14389927 | MS.gene65365:CDS | 25.0% |
| ! | CTTGGATTTGATGTTAATAT+TGG | - | chr5.1:14389809-14389828 | MS.gene65365:CDS | 25.0% |
| ! | GAATTATGTTGATCAAAAAG+GGG | - | chr5.1:14389910-14389929 | MS.gene65365:CDS | 25.0% |
| ! | TAGGTGAAATCATGTTTAAA+GGG | - | chr5.1:14390011-14390030 | MS.gene65365:CDS | 25.0% |
| ! | TTCAAATTGAAAGAACCATT+AGG | + | chr5.1:14390133-14390152 | None:intergenic | 25.0% |
| ! | TTTAAACATGATTTCACCTA+TGG | + | chr5.1:14390011-14390030 | None:intergenic | 25.0% |
| !! | TATTAGGCATTTTTCTCATA+AGG | - | chr5.1:14389727-14389746 | MS.gene65365:CDS | 25.0% |
| AGATCAATTAGATGCTCATA+TGG | - | chr5.1:14390369-14390388 | MS.gene65365:CDS | 30.0% | |
| AGTTTGCAATAATTTCCAGA+AGG | + | chr5.1:14389700-14389719 | None:intergenic | 30.0% | |
| ATAGGATCACATTCATTTCT+AGG | + | chr5.1:14389392-14389411 | None:intergenic | 30.0% | |
| GCAATTGTGAAATTGAAAGA+TGG | - | chr5.1:14390307-14390326 | MS.gene65365:CDS | 30.0% | |
| GTTTGCAATAATTTCCAGAA+GGG | + | chr5.1:14389699-14389718 | None:intergenic | 30.0% | |
| TATGGTATGACTGAAACATT+AGG | - | chr5.1:14389833-14389852 | MS.gene65365:CDS | 30.0% | |
| TGAAGCAATTCATGTTCATA+AGG | - | chr5.1:14389649-14389668 | MS.gene65365:CDS | 30.0% | |
| TTCAATTTCACAATTGCACA+AGG | + | chr5.1:14390304-14390323 | None:intergenic | 30.0% | |
| TTCCACTTTCTTACAATAAC+TGG | + | chr5.1:14389860-14389879 | None:intergenic | 30.0% | |
| TTGCAAACTGTGACATTATT+AGG | - | chr5.1:14389711-14389730 | MS.gene65365:CDS | 30.0% | |
| ! | TGATTATAAACTGCTCCTTT+TGG | + | chr5.1:14389449-14389468 | None:intergenic | 30.0% |
| !! | TGTTTTGTTGAATCATCCAA+TGG | - | chr5.1:14390216-14390235 | MS.gene65365:CDS | 30.0% |
| AAAGGGAATACTTCAATGTC+AGG | - | chr5.1:14390028-14390047 | MS.gene65365:CDS | 35.0% | |
| AAGGGAATACTTCAATGTCA+GGG | - | chr5.1:14390029-14390048 | MS.gene65365:CDS | 35.0% | |
| ACATGTTTAGATGCAATGGA+TGG | - | chr5.1:14389549-14389568 | MS.gene65365:CDS | 35.0% | |
| ATATCAGTGAACTACACATC+AGG | - | chr5.1:14389410-14389429 | MS.gene65365:CDS | 35.0% | |
| CATAAGGTAACACATTTGTG+TGG | - | chr5.1:14389665-14389684 | MS.gene65365:CDS | 35.0% | |
| GATGTGTAGTTCACTGATAT+AGG | + | chr5.1:14389410-14389429 | None:intergenic | 35.0% | |
| GGAACACTCTTCATTGTATT+TGG | + | chr5.1:14389974-14389993 | None:intergenic | 35.0% | |
| GTCCAGTTATTGTAAGAAAG+TGG | - | chr5.1:14389855-14389874 | MS.gene65365:CDS | 35.0% | |
| GTTGACATGTTTAGATGCAA+TGG | - | chr5.1:14389545-14389564 | MS.gene65365:CDS | 35.0% | |
| TAATAGTCGCGAGTGAATTA+AGG | + | chr5.1:14389484-14389503 | None:intergenic | 35.0% | |
| TGAGTTTGTGAGTTCATTAG+AGG | - | chr5.1:14390186-14390205 | MS.gene65365:CDS | 35.0% | |
| ! | AAGTGAGTCATGAAGCTTTT+AGG | - | chr5.1:14390068-14390087 | MS.gene65365:CDS | 35.0% |
| ! | ACAGCTACTTTCAAAACCAT+TGG | + | chr5.1:14390235-14390254 | None:intergenic | 35.0% |
| ! | AGTGAGTCATGAAGCTTTTA+GGG | - | chr5.1:14390069-14390088 | MS.gene65365:CDS | 35.0% |
| ! | CCATATGATGGAAAAACCAT+AGG | - | chr5.1:14389992-14390011 | MS.gene65365:CDS | 35.0% |
| ! | CCTATGGTTTTTCCATCATA+TGG | + | chr5.1:14389995-14390014 | None:intergenic | 35.0% |
| !! | AGGGGTTATTGACTTTATGA+TGG | - | chr5.1:14389928-14389947 | MS.gene65365:CDS | 35.0% |
| !! | ATCCTATGCTGGTGTTTTTA+TGG | - | chr5.1:14389519-14389538 | MS.gene65365:CDS | 35.0% |
| !! | ATGAAGAGTGTTCCATATGA+TGG | - | chr5.1:14389980-14389999 | MS.gene65365:CDS | 35.0% |
| !! | TTTTGAAAGTAGCTGTTGTG+GGG | - | chr5.1:14390239-14390258 | MS.gene65365:CDS | 35.0% |
| !!! | GTTTTGAAAGTAGCTGTTGT+GGG | - | chr5.1:14390238-14390257 | MS.gene65365:CDS | 35.0% |
| ATTTGGTTAATGCCTGCTCT+TGG | - | chr5.1:14389578-14389597 | MS.gene65365:CDS | 40.0% | |
| CATTAGGTAACCTAACACCT+AGG | + | chr5.1:14390117-14390136 | None:intergenic | 40.0% | |
| CTAGGTGTTAGGTTACCTAA+TGG | - | chr5.1:14390115-14390134 | MS.gene65365:CDS | 40.0% | |
| GCAATAATTTCCAGAAGGGT+AGG | + | chr5.1:14389695-14389714 | None:intergenic | 40.0% | |
| GCAATGGATGGTGTTTCATT+TGG | - | chr5.1:14389561-14389580 | MS.gene65365:CDS | 40.0% | |
| GTCCATAAAAACACCAGCAT+AGG | + | chr5.1:14389524-14389543 | None:intergenic | 40.0% | |
| GTTTGTGAGTTCATTAGAGG+TGG | - | chr5.1:14390189-14390208 | MS.gene65365:CDS | 40.0% | |
| TCATATGGTTCCTAAGAGTG+TGG | - | chr5.1:14390384-14390403 | MS.gene65365:CDS | 40.0% | |
| TTTCGATGTGAATCCTATGC+TGG | - | chr5.1:14389508-14389527 | MS.gene65365:CDS | 40.0% | |
| !! | AGTCATGAAGCTTTTAGGGA+TGG | - | chr5.1:14390073-14390092 | MS.gene65365:CDS | 40.0% |
| !! | ATGAAGCTTTTAGGGATGGT+TGG | - | chr5.1:14390077-14390096 | MS.gene65365:CDS | 40.0% |
| !! | CTTTTAGGGATGGTTGGTAT+AGG | - | chr5.1:14390083-14390102 | MS.gene65365:CDS | 40.0% |
| !!! | GGTTTTGAAAGTAGCTGTTG+TGG | - | chr5.1:14390237-14390256 | MS.gene65365:CDS | 40.0% |
| AACTACACATCAGGCTCAAC+TGG | - | chr5.1:14389419-14389438 | MS.gene65365:CDS | 45.0% | |
| CTTAACAAAGTGGCAGAGCT+TGG | - | chr5.1:14389791-14389810 | MS.gene65365:CDS | 45.0% | |
| GGCTCAACTGGAATTCCAAA+AGG | - | chr5.1:14389431-14389450 | MS.gene65365:CDS | 45.0% | |
| TCACCAAACACCACACTCTT+AGG | + | chr5.1:14390397-14390416 | None:intergenic | 45.0% | |
| ! | GGATGGTTGGTATAGGACTA+GGG | - | chr5.1:14390090-14390109 | MS.gene65365:CDS | 45.0% |
| ! | GTGGGGAGATATGATGATGA+TGG | - | chr5.1:14390256-14390275 | MS.gene65365:CDS | 45.0% |
| ! | GTTCCTAAGAGTGTGGTGTT+TGG | - | chr5.1:14390391-14390410 | MS.gene65365:CDS | 45.0% |
| !! | TGATGATGGTGACGAGTGTT+TGG | - | chr5.1:14390270-14390289 | MS.gene65365:CDS | 45.0% |
| AAGGTGAGTGTCACAGTTGC+TGG | - | chr5.1:14389746-14389765 | MS.gene65365:CDS | 50.0% | |
| ATGTTTGTGCCACCAAGAGC+AGG | + | chr5.1:14389593-14389612 | None:intergenic | 50.0% | |
| TGGTATAGGACTAGGGACCT+AGG | - | chr5.1:14390097-14390116 | MS.gene65365:CDS | 50.0% | |
| TGGTTAATGCCTGCTCTTGG+TGG | - | chr5.1:14389581-14389600 | MS.gene65365:CDS | 50.0% | |
| ! | GGGATGGTTGGTATAGGACT+AGG | - | chr5.1:14390089-14390108 | MS.gene65365:CDS | 50.0% |
| GGACTAGGGACCTAGGTGTT+AGG | - | chr5.1:14390104-14390123 | MS.gene65365:CDS | 55.0% | |
| GTGTGGTGCACCTACCCTTC+TGG | - | chr5.1:14389682-14389701 | MS.gene65365:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 14389386 | 14390436 | 14389386 | ID=MS.gene65365 |
| chr5.1 | mRNA | 14389386 | 14390436 | 14389386 | ID=MS.gene65365.t1;Parent=MS.gene65365 |
| chr5.1 | exon | 14389386 | 14390436 | 14389386 | ID=MS.gene65365.t1.exon1;Parent=MS.gene65365.t1 |
| chr5.1 | CDS | 14389386 | 14390436 | 14389386 | ID=cds.MS.gene65365.t1;Parent=MS.gene65365.t1 |
| Gene Sequence |
| Protein sequence |
