Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65512.t1 | XP_013467108.1 | 97.7 | 556 | 9 | 1 | 1 | 552 | 54 | 609 | 6.50E-308 | 1065.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65512.t1 | A0A072VGG8 | 97.7 | 556 | 9 | 1 | 1 | 552 | 54 | 609 | 4.7e-308 | 1065.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049983 | MS.gene65512 | 0.806871 | 6.67E-50 | -1.69E-46 |
| MS.gene050689 | MS.gene65512 | 0.804765 | 1.85E-49 | -1.69E-46 |
| MS.gene053418 | MS.gene65512 | 0.859396 | 4.26E-63 | -1.69E-46 |
| MS.gene053427 | MS.gene65512 | 0.801462 | 8.90E-49 | -1.69E-46 |
| MS.gene056834 | MS.gene65512 | 0.826906 | 2.10E-54 | -1.69E-46 |
| MS.gene057034 | MS.gene65512 | 0.807514 | 4.87E-50 | -1.69E-46 |
| MS.gene058781 | MS.gene65512 | 0.831719 | 1.42E-55 | -1.69E-46 |
| MS.gene058783 | MS.gene65512 | 0.838429 | 2.89E-57 | -1.69E-46 |
| MS.gene060935 | MS.gene65512 | 0.807783 | 4.27E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65512.t1 | MTR_1g044135 | 97.662 | 556 | 9 | 1 | 1 | 552 | 54 | 609 | 0.0 | 1127 |
| MS.gene65512.t1 | MTR_1g073890 | 68.036 | 560 | 134 | 8 | 1 | 552 | 57 | 579 | 0.0 | 754 |
| MS.gene65512.t1 | MTR_3g091180 | 37.900 | 562 | 301 | 14 | 1 | 532 | 37 | 580 | 1.01e-106 | 332 |
| MS.gene65512.t1 | MTR_1g061540 | 33.022 | 536 | 290 | 12 | 1 | 521 | 40 | 521 | 1.67e-71 | 238 |
| MS.gene65512.t1 | MTR_7g103490 | 33.515 | 549 | 289 | 14 | 1 | 523 | 40 | 538 | 2.84e-70 | 236 |
| MS.gene65512.t1 | MTR_1g092540 | 58.929 | 168 | 63 | 3 | 362 | 526 | 414 | 578 | 4.78e-56 | 198 |
| MS.gene65512.t1 | MTR_1g092540 | 35.751 | 193 | 122 | 1 | 1 | 191 | 37 | 229 | 3.42e-36 | 142 |
| MS.gene65512.t1 | MTR_0318s0020 | 53.191 | 188 | 84 | 2 | 347 | 532 | 380 | 565 | 5.75e-56 | 197 |
| MS.gene65512.t1 | MTR_0318s0020 | 36.792 | 212 | 113 | 2 | 1 | 191 | 139 | 350 | 2.54e-34 | 137 |
| MS.gene65512.t1 | MTR_6g461910 | 51.479 | 169 | 77 | 4 | 371 | 535 | 343 | 510 | 1.33e-45 | 168 |
| MS.gene65512.t1 | MTR_6g461910 | 40.513 | 195 | 112 | 2 | 1 | 191 | 47 | 241 | 5.56e-37 | 144 |
| MS.gene65512.t1 | MTR_4g021260 | 40.000 | 220 | 115 | 3 | 1 | 203 | 30 | 249 | 2.73e-42 | 158 |
| MS.gene65512.t1 | MTR_4g021260 | 43.836 | 146 | 77 | 3 | 378 | 518 | 330 | 475 | 6.34e-32 | 129 |
| MS.gene65512.t1 | MTR_7g063040 | 40.404 | 198 | 111 | 1 | 1 | 191 | 36 | 233 | 5.77e-42 | 157 |
| MS.gene65512.t1 | MTR_7g063040 | 35.586 | 222 | 110 | 10 | 312 | 524 | 286 | 483 | 1.15e-26 | 113 |
| MS.gene65512.t1 | MTR_1g096970 | 31.633 | 196 | 124 | 3 | 3 | 191 | 64 | 256 | 9.82e-18 | 86.7 |
| MS.gene65512.t1 | MTR_1g096970 | 29.054 | 148 | 91 | 2 | 381 | 518 | 269 | 412 | 1.11e-13 | 73.9 |
| MS.gene65512.t1 | MTR_3g097240 | 29.187 | 209 | 136 | 3 | 3 | 209 | 154 | 352 | 5.74e-16 | 80.5 |
| MS.gene65512.t1 | MTR_3g097240 | 29.187 | 209 | 136 | 3 | 3 | 209 | 154 | 352 | 7.26e-16 | 80.5 |
| MS.gene65512.t1 | MTR_3g097240 | 29.187 | 209 | 136 | 3 | 3 | 209 | 154 | 352 | 1.15e-15 | 80.5 |
| MS.gene65512.t1 | MTR_3g097240 | 30.882 | 136 | 77 | 4 | 383 | 507 | 359 | 488 | 7.33e-12 | 68.6 |
| MS.gene65512.t1 | MTR_1g025340 | 30.500 | 200 | 119 | 3 | 7 | 194 | 144 | 335 | 1.48e-15 | 80.1 |
| MS.gene65512.t1 | MTR_1g025340 | 29.870 | 154 | 89 | 4 | 380 | 520 | 345 | 492 | 6.36e-12 | 68.6 |
| MS.gene65512.t1 | MTR_3g114010 | 29.596 | 223 | 140 | 5 | 1 | 213 | 133 | 348 | 3.32e-15 | 79.0 |
| MS.gene65512.t1 | MTR_3g114010 | 31.646 | 158 | 93 | 3 | 380 | 526 | 340 | 493 | 7.18e-15 | 78.2 |
| MS.gene65512.t1 | MTR_8g095080 | 29.104 | 134 | 82 | 3 | 383 | 507 | 373 | 502 | 1.07e-11 | 68.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65512.t1 | AT3G23160 | 56.937 | 555 | 159 | 9 | 1 | 552 | 54 | 531 | 0.0 | 610 |
| MS.gene65512.t1 | AT5G04550 | 34.975 | 589 | 306 | 12 | 1 | 541 | 37 | 596 | 1.27e-101 | 319 |
| MS.gene65512.t1 | AT5G04550 | 34.975 | 589 | 306 | 12 | 1 | 541 | 37 | 596 | 1.27e-101 | 319 |
| MS.gene65512.t1 | AT5G51670 | 49.425 | 174 | 82 | 2 | 357 | 526 | 289 | 460 | 5.76e-48 | 174 |
| MS.gene65512.t1 | AT5G51670 | 32.212 | 208 | 127 | 1 | 1 | 194 | 48 | 255 | 1.30e-25 | 110 |
| MS.gene65512.t1 | AT5G51670 | 49.425 | 174 | 82 | 2 | 357 | 526 | 289 | 460 | 5.76e-48 | 174 |
| MS.gene65512.t1 | AT5G51670 | 32.212 | 208 | 127 | 1 | 1 | 194 | 48 | 255 | 1.30e-25 | 110 |
| MS.gene65512.t1 | AT1G34320 | 30.303 | 198 | 126 | 4 | 3 | 191 | 168 | 362 | 5.33e-17 | 84.7 |
| MS.gene65512.t1 | AT1G34320 | 31.579 | 152 | 88 | 4 | 379 | 518 | 373 | 520 | 1.51e-14 | 77.0 |
| MS.gene65512.t1 | AT1G34320 | 30.303 | 198 | 126 | 4 | 3 | 191 | 168 | 362 | 5.33e-17 | 84.7 |
| MS.gene65512.t1 | AT1G34320 | 31.579 | 152 | 88 | 4 | 379 | 518 | 373 | 520 | 1.51e-14 | 77.0 |
| MS.gene65512.t1 | AT5G08660 | 31.973 | 147 | 83 | 4 | 383 | 518 | 372 | 512 | 5.60e-14 | 75.5 |
| MS.gene65512.t1 | AT5G08660 | 31.973 | 147 | 83 | 4 | 383 | 518 | 372 | 512 | 5.60e-14 | 75.5 |
| MS.gene65512.t1 | AT5G08660 | 31.973 | 147 | 83 | 4 | 383 | 518 | 372 | 512 | 5.60e-14 | 75.5 |
| MS.gene65512.t1 | AT1G30755 | 27.317 | 205 | 139 | 2 | 1 | 198 | 145 | 346 | 4.94e-13 | 71.6 |
| MS.gene65512.t1 | AT1G30755 | 27.317 | 205 | 139 | 2 | 1 | 198 | 145 | 346 | 6.28e-13 | 72.0 |
| MS.gene65512.t1 | AT1G30755 | 27.317 | 205 | 139 | 2 | 1 | 198 | 145 | 346 | 6.28e-13 | 72.0 |
| MS.gene65512.t1 | AT1G30755 | 27.317 | 205 | 139 | 2 | 1 | 198 | 145 | 346 | 6.28e-13 | 72.0 |
| MS.gene65512.t1 | AT1G30755 | 27.317 | 205 | 139 | 2 | 1 | 198 | 145 | 346 | 6.56e-13 | 71.2 |
| MS.gene65512.t1 | AT1G30755 | 27.317 | 205 | 139 | 2 | 1 | 198 | 145 | 346 | 6.56e-13 | 71.2 |
Find 109 sgRNAs with CRISPR-Local
Find 128 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CACTGAAGGTGTTAAGAATT+TGG | 0.207485 | 1.1:-27479302 | MS.gene65512:CDS |
| TCTCTTCGGCCTGAGGATTT+TGG | 0.208197 | 1.1:-27478572 | MS.gene65512:CDS |
| GAAGTTGTATGTCAAGAATT+TGG | 0.251959 | 1.1:-27478078 | MS.gene65512:CDS |
| CAAAGTGGCGTTTCGTTTAC+CGG | 0.272203 | 1.1:-27478341 | MS.gene65512:CDS |
| GTTATTGATGTGAAGGAGTT+AGG | 0.272849 | 1.1:-27479124 | MS.gene65512:CDS |
| ATTAGTGTTCAGACGAATTC+AGG | 0.287770 | 1.1:-27478725 | MS.gene65512:CDS |
| AGGGAGCATTTGTTCCATTC+AGG | 0.298803 | 1.1:-27478380 | MS.gene65512:CDS |
| GTGTTGCTAGTGTTGTTTCT+AGG | 0.315502 | 1.1:-27479216 | MS.gene65512:CDS |
| GCTAGTGTTGTTTCTAGGTT+AGG | 0.325266 | 1.1:-27479211 | MS.gene65512:CDS |
| ATTTGAAGGATGTTTCTCTT+TGG | 0.330959 | 1.1:-27478907 | MS.gene65512:CDS |
| GGAAGGAATGGTGAGGAAAA+TGG | 0.336491 | 1.1:-27479083 | MS.gene65512:CDS |
| CCTCCCCTTCTCTGCACTGC+AGG | 0.343424 | 1.1:+27478641 | None:intergenic |
| TATGAGTTTGTATAGTGAAT+TGG | 0.345304 | 1.1:-27479041 | MS.gene65512:CDS |
| GGCAAGAGGGGCGTCATATA+TGG | 0.352595 | 1.1:+27478055 | None:intergenic |
| TCGATTTGCGATCTCCTTGT+TGG | 0.379995 | 1.1:-27477861 | MS.gene65512:CDS |
| CTTCGCTATCCTCATTTAGT+TGG | 0.380532 | 1.1:-27478170 | MS.gene65512:CDS |
| TGAGATCCATTTCTCTTGCT+TGG | 0.390123 | 1.1:+27478686 | None:intergenic |
| TTATTGATGTGAAGGAGTTA+GGG | 0.393838 | 1.1:-27479123 | MS.gene65512:CDS |
| ACGCCCCTCTTGCCCATGAT+TGG | 0.396048 | 1.1:-27478046 | MS.gene65512:CDS |
| CATTTGTCCTGCTAACTATC+TGG | 0.399209 | 1.1:+27477934 | None:intergenic |
| CGATTTGCGATCTCCTTGTT+GGG | 0.400087 | 1.1:-27477860 | MS.gene65512:CDS |
| ATTTAGTTGGCGAGGAAGCC+AGG | 0.413244 | 1.1:-27478157 | MS.gene65512:CDS |
| GCTTTGAGGAACAATAGTTT+TGG | 0.418981 | 1.1:-27478797 | MS.gene65512:CDS |
| TTGGAATCAGACATTTGATA+AGG | 0.418982 | 1.1:-27478888 | MS.gene65512:CDS |
| GCTAGAATTTCTGTGATCTT+CGG | 0.421113 | 1.1:-27478827 | MS.gene65512:CDS |
| ACAACAGCAAAATGCATTGT+TGG | 0.424680 | 1.1:-27477814 | MS.gene65512:CDS |
| CGCAATCCAAGCAAGAGAAA+TGG | 0.425439 | 1.1:-27478692 | MS.gene65512:CDS |
| GCTGAGCCACCAAGTGTTGA+AGG | 0.440558 | 1.1:+27478239 | None:intergenic |
| GATTGGAAAGAAAATCTTGA+TGG | 0.444872 | 1.1:-27478029 | MS.gene65512:CDS |
| TGATAAGGTTGTTGAGTTGT+TGG | 0.446823 | 1.1:-27478873 | MS.gene65512:CDS |
| AGAGATAACCTTAGGCTTGT+TGG | 0.449081 | 1.1:+27478113 | None:intergenic |
| TTTAGCTTGTCTTAGTTCTC+CGG | 0.450690 | 1.1:+27478322 | None:intergenic |
| TTTCCTTGTGGAACAAGTCC+GGG | 0.463603 | 1.1:-27478548 | MS.gene65512:CDS |
| TGTTCTGAGCCTGCTTTACA+AGG | 0.464946 | 1.1:-27479181 | MS.gene65512:CDS |
| TCGCCTTGAGAGATAACCTT+AGG | 0.467390 | 1.1:+27478105 | None:intergenic |
| TGTTGAAGGAGGAGCATAAA+CGG | 0.478993 | 1.1:+27478253 | None:intergenic |
| TGCTCAAACCCTTGTAAAGC+AGG | 0.479272 | 1.1:+27479172 | None:intergenic |
| AAAATCTTGATGGGATGCTT+CGG | 0.481002 | 1.1:-27478019 | MS.gene65512:CDS |
| CTCTATGACAATTATGACAT+TGG | 0.483229 | 1.1:+27478199 | None:intergenic |
| TGCGAATAGTAGCGTGAAAA+GGG | 0.485834 | 1.1:-27478399 | MS.gene65512:CDS |
| TATGCTCCTCCTTCAACACT+TGG | 0.488945 | 1.1:-27478248 | MS.gene65512:CDS |
| TTGCGAATAGTAGCGTGAAA+AGG | 0.489301 | 1.1:-27478400 | MS.gene65512:CDS |
| GCTCCTCCTTCAACACTTGG+TGG | 0.489813 | 1.1:-27478245 | MS.gene65512:CDS |
| GTTTATAGTGACATTGTTGG+TGG | 0.490426 | 1.1:-27479148 | MS.gene65512:CDS |
| CAACGTTTAGTCCGAAAAGT+AGG | 0.494561 | 1.1:-27478280 | MS.gene65512:CDS |
| GGTTAAGCATATGGAAGGAA+TGG | 0.497345 | 1.1:-27479095 | MS.gene65512:CDS |
| TCTTCGGTGAAACCGCTTTG+AGG | 0.497670 | 1.1:-27478811 | MS.gene65512:CDS |
| CAAGAGAAATGGATCTCAGC+CGG | 0.499012 | 1.1:-27478681 | MS.gene65512:CDS |
| AATTTGGTTTCATCTGATGA+AGG | 0.501783 | 1.1:-27479286 | MS.gene65512:CDS |
| GAATGCCTGCAGTGCAGAGA+AGG | 0.502514 | 1.1:-27478646 | MS.gene65512:CDS |
| CGCCACTTTGAACATGACTT+AGG | 0.513191 | 1.1:+27478354 | None:intergenic |
| CTTTCCAATCATGGGCAAGA+GGG | 0.515040 | 1.1:+27478042 | None:intergenic |
| TCGACTCCTCGGTCTTCTCA+CGG | 0.516141 | 1.1:+27477880 | None:intergenic |
| CATGTTTATAGTGACATTGT+TGG | 0.516499 | 1.1:-27479151 | MS.gene65512:CDS |
| AGCAGCACCAGATAGTTAGC+AGG | 0.517386 | 1.1:-27477941 | MS.gene65512:CDS |
| TTTCAGAGTCTGAGATCTCA+AGG | 0.524223 | 1.1:-27479345 | MS.gene65512:CDS |
| GGCATTCTTGCAACTGAACC+CGG | 0.525118 | 1.1:+27478662 | None:intergenic |
| TTATCTTCTTGAGCTTGCTA+TGG | 0.527061 | 1.1:-27479263 | MS.gene65512:CDS |
| ATCTTGATGGGATGCTTCGG+TGG | 0.527086 | 1.1:-27478016 | MS.gene65512:CDS |
| GAACTAAGACAAGCTAAATC+CGG | 0.530523 | 1.1:-27478317 | MS.gene65512:CDS |
| GTGGTGAATTAGCTTCTCTT+CGG | 0.532173 | 1.1:-27478586 | MS.gene65512:CDS |
| TTAGTGTTCAGACGAATTCA+GGG | 0.532405 | 1.1:-27478724 | MS.gene65512:CDS |
| AATGCCTGCAGTGCAGAGAA+GGG | 0.534248 | 1.1:-27478645 | MS.gene65512:CDS |
| TGGTGGTGTTATTGATGTGA+AGG | 0.536993 | 1.1:-27479131 | MS.gene65512:CDS |
| GCTTCCTCGCCAACTAAATG+AGG | 0.537140 | 1.1:+27478161 | None:intergenic |
| ATTGGAGGTGTTGAATGAGT+TGG | 0.544992 | 1.1:-27479023 | MS.gene65512:CDS |
| AAGCCTAAGGTTATCTCTCA+AGG | 0.550014 | 1.1:-27478108 | MS.gene65512:CDS |
| CAAGGAAAACCAAAATCCTC+AGG | 0.557554 | 1.1:+27478563 | None:intergenic |
| GCAAGATGTGAGACATTTGA+AGG | 0.559806 | 1.1:-27478921 | MS.gene65512:CDS |
| CCTGCAGTGCAGAGAAGGGG+AGG | 0.563007 | 1.1:-27478641 | MS.gene65512:CDS |
| CCGGTTATGCAAAATGAATG+TGG | 0.573410 | 1.1:-27478764 | MS.gene65512:CDS |
| AATGAGATCTTGAACACTGA+AGG | 0.573965 | 1.1:-27479316 | MS.gene65512:CDS |
| ATTAGCTTCTCTTCGGCCTG+AGG | 0.574077 | 1.1:-27478579 | MS.gene65512:CDS |
| AAGAGAAATGGATCTCAGCC+GGG | 0.575402 | 1.1:-27478680 | MS.gene65512:CDS |
| CTCATCATATTATGTGCAAG+AGG | 0.575455 | 1.1:+27477987 | None:intergenic |
| GAGATCGCAAATCGACTCCT+CGG | 0.578454 | 1.1:+27477869 | None:intergenic |
| TTGCACATAATATGATGAGA+TGG | 0.578599 | 1.1:-27477983 | MS.gene65512:CDS |
| CATGACTTAGGACACCTGAA+TGG | 0.579832 | 1.1:+27478366 | None:intergenic |
| AGATGCTACCAACAAGCCTA+AGG | 0.579945 | 1.1:-27478121 | MS.gene65512:CDS |
| AGGTTGTTGAGTTGTTGGCT+AGG | 0.583359 | 1.1:-27478868 | MS.gene65512:CDS |
| GAATGGTGAGGAAAATGGAT+AGG | 0.586857 | 1.1:-27479078 | MS.gene65512:CDS |
| TTTAGTTGGCGAGGAAGCCA+GGG | 0.587573 | 1.1:-27478156 | MS.gene65512:CDS |
| GAGGAGCTGCTAGTAAGCCT+CGG | 0.589855 | 1.1:-27478622 | MS.gene65512:CDS |
| ATTGGAAAGAAAATCTTGAT+GGG | 0.593898 | 1.1:-27478028 | MS.gene65512:CDS |
| TTTCCAATCATGGGCAAGAG+GGG | 0.599297 | 1.1:+27478043 | None:intergenic |
| TCTTTCCAATCATGGGCAAG+AGG | 0.602666 | 1.1:+27478041 | None:intergenic |
| CATCTGATATAGATCATCCC+TGG | 0.602981 | 1.1:+27478139 | None:intergenic |
| GAGTTTGTATAGTGAATTGG+AGG | 0.605286 | 1.1:-27479038 | MS.gene65512:CDS |
| CTATCCTCATTTAGTTGGCG+AGG | 0.607619 | 1.1:-27478165 | MS.gene65512:CDS |
| CTCCTCGGTCTTCTCACGGT+CGG | 0.611073 | 1.1:+27477884 | None:intergenic |
| AAAACTATTGTTCCTCAAAG+CGG | 0.613283 | 1.1:+27478799 | None:intergenic |
| AGATTTGATGATGTCGACGA+CGG | 0.613968 | 1.1:-27478482 | MS.gene65512:CDS |
| ATGCCTGCAGTGCAGAGAAG+GGG | 0.616055 | 1.1:-27478644 | MS.gene65512:CDS |
| CCACTTCTCATGTCAATCCG+AGG | 0.616958 | 1.1:+27478605 | None:intergenic |
| CCTCGGATTGACATGAGAAG+TGG | 0.627500 | 1.1:-27478605 | MS.gene65512:CDS |
| TTCCTTGTGGAACAAGTCCG+GGG | 0.632704 | 1.1:-27478547 | MS.gene65512:CDS |
| GTTCTGAGCCTGCTTTACAA+GGG | 0.634634 | 1.1:-27479180 | MS.gene65512:CDS |
| CTCCCCGGACTTGTTCCACA+AGG | 0.636189 | 1.1:+27478545 | None:intergenic |
| AGCATATGGAAGGAATGGTG+AGG | 0.646265 | 1.1:-27479090 | MS.gene65512:CDS |
| CGTTGAGCAACTTTGTACGC+CGG | 0.648077 | 1.1:+27478298 | None:intergenic |
| GAGCCACCAAGTGTTGAAGG+AGG | 0.655057 | 1.1:+27478242 | None:intergenic |
| GTCCTAAGTCATGTTCAAAG+TGG | 0.658478 | 1.1:-27478356 | MS.gene65512:CDS |
| GTTTCAGAATAATCAGCATG+TGG | 0.663349 | 1.1:-27478984 | MS.gene65512:CDS |
| ATATGTAATTCAACCCAACA+AGG | 0.673128 | 1.1:+27477847 | None:intergenic |
| TAGAAACAACACTAGCAACA+CGG | 0.673276 | 1.1:+27479218 | None:intergenic |
| CGGTTATGTTATCAATCACG+AGG | 0.683171 | 1.1:-27478462 | MS.gene65512:CDS |
| CATGTATCTAGCAGTCGCAG+TGG | 0.692119 | 1.1:-27478428 | MS.gene65512:CDS |
| CGCCGACCGTGAGAAGACCG+AGG | 0.715006 | 1.1:-27477886 | MS.gene65512:CDS |
| ATGGAGTGGCAATTACAATG+TGG | 0.791540 | 1.1:-27477759 | MS.gene65512:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTGATTTTGAAGATTGTA+TGG | - | chr1.1:27479338-27479357 | MS.gene65512:CDS | 20.0% |
| ! | ATTGGAAAGAAAATCTTGAT+GGG | - | chr1.1:27479088-27479107 | MS.gene65512:CDS | 25.0% |
| ! | TATGAGTTTGTATAGTGAAT+TGG | - | chr1.1:27478075-27478094 | MS.gene65512:CDS | 25.0% |
| !! | AAGATTTTCTTTCCAATCAT+GGG | + | chr1.1:27479085-27479104 | None:intergenic | 25.0% |
| !!! | AATGAATGTGGATTTTTTTC+TGG | - | chr1.1:27478364-27478383 | MS.gene65512:CDS | 25.0% |
| AAAACTATTGTTCCTCAAAG+CGG | + | chr1.1:27478320-27478339 | None:intergenic | 30.0% | |
| ATATGTAATTCAACCCAACA+AGG | + | chr1.1:27479272-27479291 | None:intergenic | 30.0% | |
| ATTTGAAGGATGTTTCTCTT+TGG | - | chr1.1:27478209-27478228 | MS.gene65512:CDS | 30.0% | |
| CATGTTTATAGTGACATTGT+TGG | - | chr1.1:27477965-27477984 | MS.gene65512:CDS | 30.0% | |
| CTCTATGACAATTATGACAT+TGG | + | chr1.1:27478920-27478939 | None:intergenic | 30.0% | |
| GAAGTTGTATGTCAAGAATT+TGG | - | chr1.1:27479038-27479057 | MS.gene65512:CDS | 30.0% | |
| GATTGGAAAGAAAATCTTGA+TGG | - | chr1.1:27479087-27479106 | MS.gene65512:CDS | 30.0% | |
| TTATTGATGTGAAGGAGTTA+GGG | - | chr1.1:27477993-27478012 | MS.gene65512:CDS | 30.0% | |
| TTGCACATAATATGATGAGA+TGG | - | chr1.1:27479133-27479152 | MS.gene65512:CDS | 30.0% | |
| TTGGAATCAGACATTTGATA+AGG | - | chr1.1:27478228-27478247 | MS.gene65512:CDS | 30.0% | |
| ! | AATTTGGTTTCATCTGATGA+AGG | - | chr1.1:27477830-27477849 | MS.gene65512:CDS | 30.0% |
| ! | CAAGATTTTCTTTCCAATCA+TGG | + | chr1.1:27479086-27479105 | None:intergenic | 30.0% |
| ! | CTTTTGAGCAGAAACTTTTA+TGG | - | chr1.1:27478167-27478186 | MS.gene65512:CDS | 30.0% |
| !! | ATTTTGAAGATTGTATGGAG+TGG | - | chr1.1:27479343-27479362 | MS.gene65512:CDS | 30.0% |
| !!! | TTTTTGGTTAAGCATATGGA+AGG | - | chr1.1:27478016-27478035 | MS.gene65512:CDS | 30.0% |
| AAAATCTTGATGGGATGCTT+CGG | - | chr1.1:27479097-27479116 | MS.gene65512:CDS | 35.0% | |
| AATGAGATCTTGAACACTGA+AGG | - | chr1.1:27477800-27477819 | MS.gene65512:CDS | 35.0% | |
| ACAACAGCAAAATGCATTGT+TGG | - | chr1.1:27479302-27479321 | MS.gene65512:CDS | 35.0% | |
| ATTAGTGTTCAGACGAATTC+AGG | - | chr1.1:27478391-27478410 | MS.gene65512:CDS | 35.0% | |
| CTCATCATATTATGTGCAAG+AGG | + | chr1.1:27479132-27479151 | None:intergenic | 35.0% | |
| GAACTAAGACAAGCTAAATC+CGG | - | chr1.1:27478799-27478818 | MS.gene65512:CDS | 35.0% | |
| GAGTTTGTATAGTGAATTGG+AGG | - | chr1.1:27478078-27478097 | MS.gene65512:CDS | 35.0% | |
| GCTAGAATTTCTGTGATCTT+CGG | - | chr1.1:27478289-27478308 | MS.gene65512:CDS | 35.0% | |
| GTTATTGATGTGAAGGAGTT+AGG | - | chr1.1:27477992-27478011 | MS.gene65512:CDS | 35.0% | |
| GTTTATAGTGACATTGTTGG+TGG | - | chr1.1:27477968-27477987 | MS.gene65512:CDS | 35.0% | |
| GTTTCAGAATAATCAGCATG+TGG | - | chr1.1:27478132-27478151 | MS.gene65512:CDS | 35.0% | |
| TAGAAACAACACTAGCAACA+CGG | + | chr1.1:27477901-27477920 | None:intergenic | 35.0% | |
| TTAGTGTTCAGACGAATTCA+GGG | - | chr1.1:27478392-27478411 | MS.gene65512:CDS | 35.0% | |
| ! | CCACATTCATTTTGCATAAC+CGG | + | chr1.1:27478355-27478374 | None:intergenic | 35.0% |
| ! | TGATAAGGTTGTTGAGTTGT+TGG | - | chr1.1:27478243-27478262 | MS.gene65512:CDS | 35.0% |
| !! | CACTGAAGGTGTTAAGAATT+TGG | - | chr1.1:27477814-27477833 | MS.gene65512:CDS | 35.0% |
| !! | TAAACGGCTAACCTACTTTT+CGG | + | chr1.1:27478850-27478869 | None:intergenic | 35.0% |
| !! | TTATCTTCTTGAGCTTGCTA+TGG | - | chr1.1:27477853-27477872 | MS.gene65512:CDS | 35.0% |
| !! | TTTAGCTTGTCTTAGTTCTC+CGG | + | chr1.1:27478797-27478816 | None:intergenic | 35.0% |
| !!! | AGGGTTTTTGGTTAAGCATA+TGG | - | chr1.1:27478012-27478031 | MS.gene65512:CDS | 35.0% |
| !!! | GCTTTGAGGAACAATAGTTT+TGG | - | chr1.1:27478319-27478338 | MS.gene65512:CDS | 35.0% |
| !!! | TTGAGGAACAATAGTTTTGG+TGG | - | chr1.1:27478322-27478341 | MS.gene65512:CDS | 35.0% |
| AAGCCTAAGGTTATCTCTCA+AGG | - | chr1.1:27479008-27479027 | MS.gene65512:CDS | 40.0% | |
| AGAGATAACCTTAGGCTTGT+TGG | + | chr1.1:27479006-27479025 | None:intergenic | 40.0% | |
| ATGGAGTGGCAATTACAATG+TGG | - | chr1.1:27479357-27479376 | MS.gene65512:CDS | 40.0% | |
| CAACGTTTAGTCCGAAAAGT+AGG | - | chr1.1:27478836-27478855 | MS.gene65512:CDS | 40.0% | |
| CAAGGAAAACCAAAATCCTC+AGG | + | chr1.1:27478556-27478575 | None:intergenic | 40.0% | |
| CATCTGATATAGATCATCCC+TGG | + | chr1.1:27478980-27478999 | None:intergenic | 40.0% | |
| CATTTGTCCTGCTAACTATC+TGG | + | chr1.1:27479185-27479204 | None:intergenic | 40.0% | |
| CCGGTTATGCAAAATGAATG+TGG | - | chr1.1:27478352-27478371 | MS.gene65512:CDS | 40.0% | |
| CGGTTATGTTATCAATCACG+AGG | - | chr1.1:27478654-27478673 | MS.gene65512:CDS | 40.0% | |
| CTTCGCTATCCTCATTTAGT+TGG | - | chr1.1:27478946-27478965 | MS.gene65512:CDS | 40.0% | |
| GAATGGTGAGGAAAATGGAT+AGG | - | chr1.1:27478038-27478057 | MS.gene65512:CDS | 40.0% | |
| GCAAGATGTGAGACATTTGA+AGG | - | chr1.1:27478195-27478214 | MS.gene65512:CDS | 40.0% | |
| GGTTAAGCATATGGAAGGAA+TGG | - | chr1.1:27478021-27478040 | MS.gene65512:CDS | 40.0% | |
| GTCCTAAGTCATGTTCAAAG+TGG | - | chr1.1:27478760-27478779 | MS.gene65512:CDS | 40.0% | |
| TGAGATCCATTTCTCTTGCT+TGG | + | chr1.1:27478433-27478452 | None:intergenic | 40.0% | |
| TGCGAATAGTAGCGTGAAAA+GGG | - | chr1.1:27478717-27478736 | MS.gene65512:CDS | 40.0% | |
| TTGCGAATAGTAGCGTGAAA+AGG | - | chr1.1:27478716-27478735 | MS.gene65512:CDS | 40.0% | |
| ! | AGCAGAAACTTTTATGGCAG+AGG | - | chr1.1:27478173-27478192 | MS.gene65512:CDS | 40.0% |
| ! | GTGGTGAATTAGCTTCTCTT+CGG | - | chr1.1:27478530-27478549 | MS.gene65512:CDS | 40.0% |
| ! | TGTGAAGGAGTTAGGGTTTT+TGG | - | chr1.1:27478000-27478019 | MS.gene65512:CDS | 40.0% |
| ! | TGTTGAAGGAGGAGCATAAA+CGG | + | chr1.1:27478866-27478885 | None:intergenic | 40.0% |
| ! | TTTCAGAGTCTGAGATCTCA+AGG | - | chr1.1:27477771-27477790 | MS.gene65512:CDS | 40.0% |
| ! | TTTTCCTTGTGGAACAAGTC+CGG | - | chr1.1:27478567-27478586 | MS.gene65512:CDS | 40.0% |
| !! | AGATTTGATGATGTCGACGA+CGG | - | chr1.1:27478634-27478653 | MS.gene65512:CDS | 40.0% |
| !! | ATTGGAGGTGTTGAATGAGT+TGG | - | chr1.1:27478093-27478112 | MS.gene65512:CDS | 40.0% |
| !! | GCTAGTGTTGTTTCTAGGTT+AGG | - | chr1.1:27477905-27477924 | MS.gene65512:CDS | 40.0% |
| !! | GTGTTGCTAGTGTTGTTTCT+AGG | - | chr1.1:27477900-27477919 | MS.gene65512:CDS | 40.0% |
| !! | TGGTGGTGTTATTGATGTGA+AGG | - | chr1.1:27477985-27478004 | MS.gene65512:CDS | 40.0% |
| !!! | AGGAACAATAGTTTTGGTGG+TGG | - | chr1.1:27478325-27478344 | MS.gene65512:CDS | 40.0% |
| !!! | GAGGATTTTGGTTTTCCTTG+TGG | - | chr1.1:27478556-27478575 | MS.gene65512:CDS | 40.0% |
| AAGAGAAATGGATCTCAGCC+GGG | - | chr1.1:27478436-27478455 | MS.gene65512:CDS | 45.0% | |
| AGATGCTACCAACAAGCCTA+AGG | - | chr1.1:27478995-27479014 | MS.gene65512:CDS | 45.0% | |
| AGCATATGGAAGGAATGGTG+AGG | - | chr1.1:27478026-27478045 | MS.gene65512:CDS | 45.0% | |
| CAAAGTGGCGTTTCGTTTAC+CGG | - | chr1.1:27478775-27478794 | MS.gene65512:CDS | 45.0% | |
| CAAGAGAAATGGATCTCAGC+CGG | - | chr1.1:27478435-27478454 | MS.gene65512:CDS | 45.0% | |
| CACTCCATAAAAAGTCTCCC+CGG | + | chr1.1:27478589-27478608 | None:intergenic | 45.0% | |
| CATGACTTAGGACACCTGAA+TGG | + | chr1.1:27478753-27478772 | None:intergenic | 45.0% | |
| CGATTTGCGATCTCCTTGTT+GGG | - | chr1.1:27479256-27479275 | MS.gene65512:CDS | 45.0% | |
| CGCAATCCAAGCAAGAGAAA+TGG | - | chr1.1:27478424-27478443 | MS.gene65512:CDS | 45.0% | |
| CTATCCTCATTTAGTTGGCG+AGG | - | chr1.1:27478951-27478970 | MS.gene65512:CDS | 45.0% | |
| CTTTCCAATCATGGGCAAGA+GGG | + | chr1.1:27479077-27479096 | None:intergenic | 45.0% | |
| GGAAGGAATGGTGAGGAAAA+TGG | - | chr1.1:27478033-27478052 | MS.gene65512:CDS | 45.0% | |
| TATGCTCCTCCTTCAACACT+TGG | - | chr1.1:27478868-27478887 | MS.gene65512:CDS | 45.0% | |
| TCGCCTTGAGAGATAACCTT+AGG | + | chr1.1:27479014-27479033 | None:intergenic | 45.0% | |
| TCTTTCCAATCATGGGCAAG+AGG | + | chr1.1:27479078-27479097 | None:intergenic | 45.0% | |
| TGCTCAAACCCTTGTAAAGC+AGG | + | chr1.1:27477947-27477966 | None:intergenic | 45.0% | |
| TTTCCAATCATGGGCAAGAG+GGG | + | chr1.1:27479076-27479095 | None:intergenic | 45.0% | |
| TTTCCTTGTGGAACAAGTCC+GGG | - | chr1.1:27478568-27478587 | MS.gene65512:CDS | 45.0% | |
| ! | AAGTCCGGGGAGACTTTTTA+TGG | - | chr1.1:27478582-27478601 | MS.gene65512:CDS | 45.0% |
| ! | AGGTTGTTGAGTTGTTGGCT+AGG | - | chr1.1:27478248-27478267 | MS.gene65512:CDS | 45.0% |
| ! | CGCCACTTTGAACATGACTT+AGG | + | chr1.1:27478765-27478784 | None:intergenic | 45.0% |
| ! | GTTCTGAGCCTGCTTTACAA+GGG | - | chr1.1:27477936-27477955 | MS.gene65512:CDS | 45.0% |
| ! | TCGATTTGCGATCTCCTTGT+TGG | - | chr1.1:27479255-27479274 | MS.gene65512:CDS | 45.0% |
| ! | TGTTCTGAGCCTGCTTTACA+AGG | - | chr1.1:27477935-27477954 | MS.gene65512:CDS | 45.0% |
| !! | AGGGAGCATTTGTTCCATTC+AGG | - | chr1.1:27478736-27478755 | MS.gene65512:CDS | 45.0% |
| AATGCCTGCAGTGCAGAGAA+GGG | - | chr1.1:27478471-27478490 | MS.gene65512:CDS | 50.0% | |
| AGCAGCACCAGATAGTTAGC+AGG | - | chr1.1:27479175-27479194 | MS.gene65512:CDS | 50.0% | |
| ATCTTGATGGGATGCTTCGG+TGG | - | chr1.1:27479100-27479119 | MS.gene65512:CDS | 50.0% | |
| ATTTAGTTGGCGAGGAAGCC+AGG | - | chr1.1:27478959-27478978 | MS.gene65512:CDS | 50.0% | |
| CATGTATCTAGCAGTCGCAG+TGG | - | chr1.1:27478688-27478707 | MS.gene65512:CDS | 50.0% | |
| CCACTTCTCATGTCAATCCG+AGG | + | chr1.1:27478514-27478533 | None:intergenic | 50.0% | |
| CCTCGGATTGACATGAGAAG+TGG | - | chr1.1:27478511-27478530 | MS.gene65512:CDS | 50.0% | |
| CGTTGAGCAACTTTGTACGC+CGG | + | chr1.1:27478821-27478840 | None:intergenic | 50.0% | |
| GCTTCCTCGCCAACTAAATG+AGG | + | chr1.1:27478958-27478977 | None:intergenic | 50.0% | |
| GGCATTCTTGCAACTGAACC+CGG | + | chr1.1:27478457-27478476 | None:intergenic | 50.0% | |
| TCTTCGGTGAAACCGCTTTG+AGG | - | chr1.1:27478305-27478324 | MS.gene65512:CDS | 50.0% | |
| TTCCTTGTGGAACAAGTCCG+GGG | - | chr1.1:27478569-27478588 | MS.gene65512:CDS | 50.0% | |
| TTTAGTTGGCGAGGAAGCCA+GGG | - | chr1.1:27478960-27478979 | MS.gene65512:CDS | 50.0% | |
| ! | ATTAGCTTCTCTTCGGCCTG+AGG | - | chr1.1:27478537-27478556 | MS.gene65512:CDS | 50.0% |
| ! | GAGATCGCAAATCGACTCCT+CGG | + | chr1.1:27479250-27479269 | None:intergenic | 50.0% |
| ! | TCTCTTCGGCCTGAGGATTT+TGG | - | chr1.1:27478544-27478563 | MS.gene65512:CDS | 50.0% |
| !!! | TAGTTTTGGTGGTGGTTCGC+CGG | - | chr1.1:27478333-27478352 | MS.gene65512:CDS | 50.0% |
| ATGCCTGCAGTGCAGAGAAG+GGG | - | chr1.1:27478472-27478491 | MS.gene65512:CDS | 55.0% | |
| GAATGCCTGCAGTGCAGAGA+AGG | - | chr1.1:27478470-27478489 | MS.gene65512:CDS | 55.0% | |
| GAGGAGCTGCTAGTAAGCCT+CGG | - | chr1.1:27478494-27478513 | MS.gene65512:CDS | 55.0% | |
| GCTCCTCCTTCAACACTTGG+TGG | - | chr1.1:27478871-27478890 | MS.gene65512:CDS | 55.0% | |
| TCGACTCCTCGGTCTTCTCA+CGG | + | chr1.1:27479239-27479258 | None:intergenic | 55.0% | |
| !! | GAGCCACCAAGTGTTGAAGG+AGG | + | chr1.1:27478877-27478896 | None:intergenic | 55.0% |
| !! | GCTGAGCCACCAAGTGTTGA+AGG | + | chr1.1:27478880-27478899 | None:intergenic | 55.0% |
| !! | GGCAAGAGGGGCGTCATATA+TGG | + | chr1.1:27479064-27479083 | None:intergenic | 55.0% |
| ACGCCCCTCTTGCCCATGAT+TGG | - | chr1.1:27479070-27479089 | MS.gene65512:CDS | 60.0% | |
| CTCCTCGGTCTTCTCACGGT+CGG | + | chr1.1:27479235-27479254 | None:intergenic | 60.0% | |
| ! | CTCCCCGGACTTGTTCCACA+AGG | + | chr1.1:27478574-27478593 | None:intergenic | 60.0% |
| CCTCCCCTTCTCTGCACTGC+AGG | + | chr1.1:27478478-27478497 | None:intergenic | 65.0% | |
| CCTGCAGTGCAGAGAAGGGG+AGG | - | chr1.1:27478475-27478494 | MS.gene65512:CDS | 65.0% | |
| CGCCGACCGTGAGAAGACCG+AGG | - | chr1.1:27479230-27479249 | MS.gene65512:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 27477740 | 27479398 | 27477740 | ID=MS.gene65512 |
| chr1.1 | mRNA | 27477740 | 27479398 | 27477740 | ID=MS.gene65512.t1;Parent=MS.gene65512 |
| chr1.1 | exon | 27477740 | 27479398 | 27477740 | ID=MS.gene65512.t1.exon1;Parent=MS.gene65512.t1 |
| chr1.1 | CDS | 27477740 | 27479398 | 27477740 | ID=cds.MS.gene65512.t1;Parent=MS.gene65512.t1 |
| Gene Sequence |
| Protein sequence |
