Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053418.t1 | XP_013467108.1 | 97.7 | 609 | 10 | 1 | 1 | 605 | 1 | 609 | 0.00E+00 | 1166.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053418.t1 | A0A072VGG8 | 97.7 | 609 | 10 | 1 | 1 | 605 | 1 | 609 | 0.0e+00 | 1166.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene053418 | MS.gene058781 | 0.800108 | 1.68E-48 | -1.69E-46 |
| MS.gene053418 | MS.gene060522 | 0.804209 | 2.41E-49 | -1.69E-46 |
| MS.gene053418 | MS.gene062870 | 0.86022 | 2.40E-63 | -1.69E-46 |
| MS.gene053418 | MS.gene069289 | 0.800662 | 1.30E-48 | -1.69E-46 |
| MS.gene053418 | MS.gene65512 | 0.859396 | 4.26E-63 | -1.69E-46 |
| MS.gene053418 | MS.gene65513 | 0.801944 | 7.09E-49 | -1.69E-46 |
| MS.gene053418 | MS.gene74383 | 0.804864 | 1.76E-49 | -1.69E-46 |
| MS.gene053418 | MS.gene84733 | 0.815124 | 1.09E-51 | -1.69E-46 |
| MS.gene053418 | MS.gene84734 | 0.820259 | 7.54E-53 | -1.69E-46 |
| MS.gene053418 | MS.gene84866 | 0.818669 | 1.74E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053418.t1 | MTR_1g044135 | 97.701 | 609 | 10 | 1 | 1 | 605 | 1 | 609 | 0.0 | 1232 |
| MS.gene053418.t1 | MTR_1g073890 | 69.156 | 616 | 142 | 10 | 1 | 605 | 1 | 579 | 0.0 | 833 |
| MS.gene053418.t1 | MTR_3g091180 | 37.236 | 615 | 321 | 16 | 1 | 585 | 1 | 580 | 4.42e-114 | 353 |
| MS.gene053418.t1 | MTR_1g061540 | 33.877 | 552 | 295 | 13 | 38 | 574 | 25 | 521 | 3.03e-77 | 254 |
| MS.gene053418.t1 | MTR_7g103490 | 33.862 | 567 | 295 | 12 | 38 | 576 | 24 | 538 | 5.40e-76 | 252 |
| MS.gene053418.t1 | MTR_0318s0020 | 53.191 | 188 | 84 | 2 | 400 | 585 | 380 | 565 | 1.29e-55 | 198 |
| MS.gene053418.t1 | MTR_0318s0020 | 35.094 | 265 | 146 | 4 | 1 | 244 | 91 | 350 | 7.80e-40 | 154 |
| MS.gene053418.t1 | MTR_1g092540 | 58.929 | 168 | 63 | 3 | 415 | 579 | 414 | 578 | 1.42e-55 | 198 |
| MS.gene053418.t1 | MTR_1g092540 | 33.740 | 246 | 144 | 2 | 1 | 244 | 1 | 229 | 1.38e-41 | 159 |
| MS.gene053418.t1 | MTR_4g021260 | 39.749 | 239 | 127 | 3 | 35 | 256 | 11 | 249 | 2.47e-47 | 173 |
| MS.gene053418.t1 | MTR_4g021260 | 43.836 | 146 | 77 | 3 | 431 | 571 | 330 | 475 | 7.70e-32 | 129 |
| MS.gene053418.t1 | MTR_7g063040 | 40.758 | 211 | 118 | 1 | 41 | 244 | 23 | 233 | 1.37e-46 | 171 |
| MS.gene053418.t1 | MTR_7g063040 | 38.065 | 155 | 86 | 3 | 431 | 577 | 331 | 483 | 1.67e-26 | 113 |
| MS.gene053418.t1 | MTR_6g461910 | 51.479 | 169 | 77 | 4 | 424 | 588 | 343 | 510 | 1.96e-45 | 169 |
| MS.gene053418.t1 | MTR_6g461910 | 41.429 | 210 | 119 | 2 | 39 | 244 | 32 | 241 | 2.78e-42 | 160 |
| MS.gene053418.t1 | MTR_1g096970 | 33.493 | 209 | 129 | 3 | 43 | 244 | 51 | 256 | 1.14e-22 | 102 |
| MS.gene053418.t1 | MTR_1g096970 | 29.054 | 148 | 91 | 2 | 434 | 571 | 269 | 412 | 1.41e-13 | 73.9 |
| MS.gene053418.t1 | MTR_3g097240 | 31.081 | 222 | 141 | 3 | 43 | 262 | 141 | 352 | 9.32e-21 | 95.5 |
| MS.gene053418.t1 | MTR_3g097240 | 31.081 | 222 | 141 | 3 | 43 | 262 | 141 | 352 | 9.50e-21 | 95.9 |
| MS.gene053418.t1 | MTR_3g097240 | 31.081 | 222 | 141 | 3 | 43 | 262 | 141 | 352 | 2.24e-20 | 95.9 |
| MS.gene053418.t1 | MTR_3g097240 | 30.882 | 136 | 77 | 4 | 436 | 560 | 359 | 488 | 9.25e-12 | 68.6 |
| MS.gene053418.t1 | MTR_3g114010 | 31.624 | 234 | 143 | 5 | 43 | 266 | 122 | 348 | 5.44e-20 | 94.4 |
| MS.gene053418.t1 | MTR_3g114010 | 31.646 | 158 | 93 | 3 | 433 | 579 | 340 | 493 | 9.54e-15 | 77.8 |
| MS.gene053418.t1 | MTR_1g025340 | 31.364 | 220 | 131 | 3 | 40 | 247 | 124 | 335 | 5.77e-20 | 94.4 |
| MS.gene053418.t1 | MTR_1g025340 | 29.870 | 154 | 89 | 4 | 433 | 573 | 345 | 492 | 8.02e-12 | 68.6 |
| MS.gene053418.t1 | MTR_8g095080 | 29.665 | 209 | 137 | 2 | 43 | 244 | 150 | 355 | 9.81e-14 | 74.3 |
| MS.gene053418.t1 | MTR_8g095080 | 29.665 | 209 | 137 | 2 | 43 | 244 | 150 | 355 | 3.09e-13 | 73.2 |
| MS.gene053418.t1 | MTR_8g095080 | 29.104 | 134 | 82 | 3 | 436 | 560 | 373 | 502 | 1.49e-11 | 67.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053418.t1 | AT3G23160 | 58.224 | 608 | 174 | 9 | 1 | 605 | 1 | 531 | 0.0 | 680 |
| MS.gene053418.t1 | AT5G04550 | 54.438 | 169 | 72 | 2 | 431 | 594 | 428 | 596 | 2.21e-56 | 201 |
| MS.gene053418.t1 | AT5G04550 | 39.352 | 216 | 128 | 2 | 30 | 244 | 15 | 228 | 1.97e-43 | 164 |
| MS.gene053418.t1 | AT5G04550 | 54.438 | 169 | 72 | 2 | 431 | 594 | 428 | 596 | 2.21e-56 | 201 |
| MS.gene053418.t1 | AT5G04550 | 39.352 | 216 | 128 | 2 | 30 | 244 | 15 | 228 | 1.97e-43 | 164 |
| MS.gene053418.t1 | AT5G51670 | 49.425 | 174 | 82 | 2 | 410 | 579 | 289 | 460 | 9.69e-48 | 174 |
| MS.gene053418.t1 | AT5G51670 | 31.179 | 263 | 157 | 3 | 1 | 247 | 1 | 255 | 9.49e-33 | 132 |
| MS.gene053418.t1 | AT5G51670 | 49.425 | 174 | 82 | 2 | 410 | 579 | 289 | 460 | 9.69e-48 | 174 |
| MS.gene053418.t1 | AT5G51670 | 31.179 | 263 | 157 | 3 | 1 | 247 | 1 | 255 | 9.49e-33 | 132 |
| MS.gene053418.t1 | AT1G34320 | 32.701 | 211 | 130 | 4 | 43 | 244 | 155 | 362 | 3.61e-22 | 101 |
| MS.gene053418.t1 | AT1G34320 | 31.579 | 152 | 88 | 4 | 432 | 571 | 373 | 520 | 1.91e-14 | 77.0 |
| MS.gene053418.t1 | AT1G34320 | 32.701 | 211 | 130 | 4 | 43 | 244 | 155 | 362 | 3.61e-22 | 101 |
| MS.gene053418.t1 | AT1G34320 | 31.579 | 152 | 88 | 4 | 432 | 571 | 373 | 520 | 1.91e-14 | 77.0 |
| MS.gene053418.t1 | AT1G30755 | 29.167 | 216 | 143 | 2 | 43 | 251 | 134 | 346 | 1.56e-17 | 85.9 |
| MS.gene053418.t1 | AT1G30755 | 29.167 | 216 | 143 | 2 | 43 | 251 | 134 | 346 | 2.00e-17 | 85.5 |
| MS.gene053418.t1 | AT1G30755 | 29.167 | 216 | 143 | 2 | 43 | 251 | 134 | 346 | 2.00e-17 | 85.5 |
| MS.gene053418.t1 | AT1G30755 | 29.167 | 216 | 143 | 2 | 43 | 251 | 134 | 346 | 2.18e-17 | 86.3 |
| MS.gene053418.t1 | AT1G30755 | 29.167 | 216 | 143 | 2 | 43 | 251 | 134 | 346 | 2.18e-17 | 86.3 |
| MS.gene053418.t1 | AT1G30755 | 29.167 | 216 | 143 | 2 | 43 | 251 | 134 | 346 | 2.18e-17 | 86.3 |
| MS.gene053418.t1 | AT5G08660 | 34.434 | 212 | 123 | 4 | 43 | 244 | 149 | 354 | 2.08e-14 | 77.0 |
| MS.gene053418.t1 | AT5G08660 | 31.973 | 147 | 83 | 4 | 436 | 571 | 372 | 512 | 8.58e-14 | 75.1 |
| MS.gene053418.t1 | AT5G08660 | 34.434 | 212 | 123 | 4 | 43 | 244 | 149 | 354 | 2.08e-14 | 77.0 |
| MS.gene053418.t1 | AT5G08660 | 31.973 | 147 | 83 | 4 | 436 | 571 | 372 | 512 | 8.58e-14 | 75.1 |
| MS.gene053418.t1 | AT5G08660 | 34.434 | 212 | 123 | 4 | 43 | 244 | 149 | 354 | 2.08e-14 | 77.0 |
| MS.gene053418.t1 | AT5G08660 | 31.973 | 147 | 83 | 4 | 436 | 571 | 372 | 512 | 8.58e-14 | 75.1 |
| MS.gene053418.t1 | AT5G08660 | 34.434 | 212 | 123 | 4 | 43 | 244 | 149 | 354 | 2.13e-14 | 76.3 |
Find 111 sgRNAs with CRISPR-Local
Find 131 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTAATGTTCAGACGAATTC+AGG | 0.187275 | 1.4:-28363920 | MS.gene053418:CDS |
| CACTGAAGGTGTTAAGAATT+TGG | 0.207485 | 1.4:-28364497 | MS.gene053418:CDS |
| TCTCTTCGGCCTGAGGATTT+TGG | 0.208197 | 1.4:-28363767 | MS.gene053418:CDS |
| GAAGTTGTATGTCAAGAATT+TGG | 0.251959 | 1.4:-28363273 | MS.gene053418:CDS |
| GTTATTGATGTGAAGGAGTT+AGG | 0.272849 | 1.4:-28364319 | MS.gene053418:CDS |
| AGGGAGCATTTGTTCCATTC+AGG | 0.298803 | 1.4:-28363575 | MS.gene053418:CDS |
| GTGTTGCTAGTGTTGTTTCT+AGG | 0.315502 | 1.4:-28364411 | MS.gene053418:CDS |
| GCTAGTGTTGTTTCTAGGTT+AGG | 0.325266 | 1.4:-28364406 | MS.gene053418:CDS |
| ATTTGAAGGATGTTTCTCTT+TGG | 0.330959 | 1.4:-28364102 | MS.gene053418:CDS |
| GGAAGGAATGGTGAGGAAAA+TGG | 0.336491 | 1.4:-28364278 | MS.gene053418:CDS |
| TATGAGTTTGTATAGTGAAT+TGG | 0.345304 | 1.4:-28364236 | MS.gene053418:CDS |
| GGCAAGAGGGGCGTCATATA+TGG | 0.352595 | 1.4:+28363250 | None:intergenic |
| TGAGATCCATTTCTCTTGCT+TGG | 0.363534 | 1.4:+28363881 | None:intergenic |
| TCGATTTGCGATCTCCTTGT+TGG | 0.379995 | 1.4:-28363056 | MS.gene053418:CDS |
| CTTCGCTATCCTCATTTAGT+TGG | 0.380532 | 1.4:-28363365 | MS.gene053418:CDS |
| GCAACTTTGTACGCCCGATT+TGG | 0.390794 | 1.4:+28363499 | None:intergenic |
| TTATTGATGTGAAGGAGTTA+GGG | 0.393838 | 1.4:-28364318 | MS.gene053418:CDS |
| GTTTCAGATTTGAACTTACT+TGG | 0.395296 | 1.4:+28364695 | None:intergenic |
| ACGCCCCTCTTGCCCATGAT+TGG | 0.396048 | 1.4:-28363241 | MS.gene053418:CDS |
| CATTTGTCCTGCTAACTATC+TGG | 0.399209 | 1.4:+28363129 | None:intergenic |
| CGATTTGCGATCTCCTTGTT+GGG | 0.400087 | 1.4:-28363055 | MS.gene053418:CDS |
| TTTGGCTTGTCTTAGTTCTC+TGG | 0.401729 | 1.4:+28363517 | None:intergenic |
| ATTTAGTTGGCGAGGAAGCC+AGG | 0.413244 | 1.4:-28363352 | MS.gene053418:CDS |
| GCTTTGAGGAACAATAGTTT+TGG | 0.418981 | 1.4:-28363992 | MS.gene053418:CDS |
| TTGGAATCAGACATTTGATA+AGG | 0.418982 | 1.4:-28364083 | MS.gene053418:CDS |
| GCTAGAATTTCTGTGATCTT+CGG | 0.421113 | 1.4:-28364022 | MS.gene053418:CDS |
| TTTCCTTGCGGAACAAGTCC+GGG | 0.424521 | 1.4:-28363743 | MS.gene053418:CDS |
| ACAACAGCAAAATGCATTGT+TGG | 0.424680 | 1.4:-28363009 | MS.gene053418:CDS |
| CGCAATCCAAGCAAGAGAAA+TGG | 0.425439 | 1.4:-28363887 | MS.gene053418:CDS |
| AGAACTAAGACAAGCCAAAT+CGG | 0.434804 | 1.4:-28363513 | MS.gene053418:CDS |
| CAAGAGAAATGGATCTCATC+CGG | 0.438574 | 1.4:-28363876 | MS.gene053418:CDS |
| GCTGAGCCACCAAGTGTTGA+AGG | 0.440558 | 1.4:+28363434 | None:intergenic |
| CCTCCCCTTCTCTGCACGAC+AGG | 0.443784 | 1.4:+28363836 | None:intergenic |
| GATTGGAAAGAAAATCTTGA+TGG | 0.444872 | 1.4:-28363224 | MS.gene053418:CDS |
| TGATAAGGTTGTTGAGTTGT+TGG | 0.446823 | 1.4:-28364068 | MS.gene053418:CDS |
| AGAGATAACCTTAGGCTTGT+TGG | 0.449081 | 1.4:+28363308 | None:intergenic |
| ACAGAGAATAAAGAAACTAT+AGG | 0.464560 | 1.4:-28364622 | MS.gene053418:CDS |
| TGTTCTGAGCCTGCTTTACA+AGG | 0.464946 | 1.4:-28364376 | MS.gene053418:CDS |
| TCGCCTTGAGAGATAACCTT+AGG | 0.467390 | 1.4:+28363300 | None:intergenic |
| TGTTGAAGGAGGAGCATAAA+CGG | 0.478993 | 1.4:+28363448 | None:intergenic |
| TGCTCAAACCCTTGTAAAGC+AGG | 0.479272 | 1.4:+28364367 | None:intergenic |
| AAAATCTTGATGGGATGCTT+CGG | 0.481002 | 1.4:-28363214 | MS.gene053418:CDS |
| CTCTATGACAATTATGACAT+TGG | 0.483229 | 1.4:+28363394 | None:intergenic |
| TGCGAATAGTAGCGTGAAAA+GGG | 0.485834 | 1.4:-28363594 | MS.gene053418:CDS |
| TATGCTCCTCCTTCAACACT+TGG | 0.488945 | 1.4:-28363443 | MS.gene053418:CDS |
| TTGCGAATAGTAGCGTGAAA+AGG | 0.489301 | 1.4:-28363595 | MS.gene053418:CDS |
| GCTCCTCCTTCAACACTTGG+TGG | 0.489813 | 1.4:-28363440 | MS.gene053418:CDS |
| GTTTATAGTGACATTGTTGG+TGG | 0.490426 | 1.4:-28364343 | MS.gene053418:CDS |
| CAACGTTTAGTCCGAAAAGT+AGG | 0.494561 | 1.4:-28363475 | MS.gene053418:CDS |
| GGTTAAGCATATGGAAGGAA+TGG | 0.497345 | 1.4:-28364290 | MS.gene053418:CDS |
| TCTTCGGTGAAACCGCTTTG+AGG | 0.497670 | 1.4:-28364006 | MS.gene053418:CDS |
| GAATGCCTGTCGTGCAGAGA+AGG | 0.502514 | 1.4:-28363841 | MS.gene053418:CDS |
| GAACTAAGACAAGCCAAATC+GGG | 0.502976 | 1.4:-28363512 | MS.gene053418:CDS |
| CAGATATGGTAGCAGAAGCT+TGG | 0.506045 | 1.4:-28364735 | None:intergenic |
| AATTTGGTTTCGTCTGATGA+AGG | 0.508794 | 1.4:-28364481 | MS.gene053418:CDS |
| CGCCACTTTGAACATGACTT+AGG | 0.513191 | 1.4:+28363549 | None:intergenic |
| CTTTCCAATCATGGGCAAGA+GGG | 0.515040 | 1.4:+28363237 | None:intergenic |
| TCGACTCCTCGGTCTTCTCA+CGG | 0.516141 | 1.4:+28363075 | None:intergenic |
| CATGTTTATAGTGACATTGT+TGG | 0.516499 | 1.4:-28364346 | MS.gene053418:CDS |
| AGCAGCACCAGATAGTTAGC+AGG | 0.517386 | 1.4:-28363136 | MS.gene053418:CDS |
| GTGGCGAATTAGCTTCTCTT+CGG | 0.521215 | 1.4:-28363781 | MS.gene053418:CDS |
| TTAATGTTCAGACGAATTCA+GGG | 0.523346 | 1.4:-28363919 | MS.gene053418:CDS |
| TTTCAGAGTCTGAGATCTCA+AGG | 0.524223 | 1.4:-28364540 | MS.gene053418:CDS |
| TTATCTTCTTGAGCTTGCTA+TGG | 0.527061 | 1.4:-28364458 | MS.gene053418:CDS |
| ATCTTGATGGGATGCTTCGG+TGG | 0.527086 | 1.4:-28363211 | MS.gene053418:CDS |
| GGCATTCTTGCAATCGAACC+CGG | 0.533040 | 1.4:+28363857 | None:intergenic |
| TGGTGGTGTTATTGATGTGA+AGG | 0.536993 | 1.4:-28364326 | MS.gene053418:CDS |
| GCTTCCTCGCCAACTAAATG+AGG | 0.537140 | 1.4:+28363356 | None:intergenic |
| AATGCCTGTCGTGCAGAGAA+GGG | 0.539677 | 1.4:-28363840 | MS.gene053418:CDS |
| CAAGGAAAACCAAAATCCTC+AGG | 0.540929 | 1.4:+28363758 | None:intergenic |
| ATTGGAGGTGTTGAATGAGT+TGG | 0.544992 | 1.4:-28364218 | MS.gene053418:CDS |
| AAGCCTAAGGTTATCTCTCA+AGG | 0.550014 | 1.4:-28363303 | MS.gene053418:CDS |
| ATTAGCTTCTCTTCGGCCTG+AGG | 0.557655 | 1.4:-28363774 | MS.gene053418:CDS |
| GCAAGATGTGAGACATTTGA+AGG | 0.559806 | 1.4:-28364116 | MS.gene053418:CDS |
| CCGGTTATGCAAAATGAATG+TGG | 0.573410 | 1.4:-28363959 | MS.gene053418:CDS |
| AATGAGATCTTGAACACTGA+AGG | 0.573965 | 1.4:-28364511 | MS.gene053418:CDS |
| CTCATCATATTATGTGCAAG+AGG | 0.575455 | 1.4:+28363182 | None:intergenic |
| GAGATCGCAAATCGACTCCT+CGG | 0.578454 | 1.4:+28363064 | None:intergenic |
| TTGCACATAATATGATGAGA+TGG | 0.578599 | 1.4:-28363178 | MS.gene053418:CDS |
| CATGACTTAGGACACCTGAA+TGG | 0.579832 | 1.4:+28363561 | None:intergenic |
| AGATGCTACCAACAAGCCTA+AGG | 0.579945 | 1.4:-28363316 | MS.gene053418:CDS |
| ATGCCTGTCGTGCAGAGAAG+GGG | 0.582756 | 1.4:-28363839 | MS.gene053418:CDS |
| AGGTTGTTGAGTTGTTGGCT+AGG | 0.583359 | 1.4:-28364063 | MS.gene053418:CDS |
| AAGAGAAATGGATCTCATCC+GGG | 0.585608 | 1.4:-28363875 | MS.gene053418:CDS |
| GAATGGTGAGGAAAATGGAT+AGG | 0.586857 | 1.4:-28364273 | MS.gene053418:CDS |
| TTTAGTTGGCGAGGAAGCCA+GGG | 0.587573 | 1.4:-28363351 | MS.gene053418:CDS |
| CCACTTCTCATGTCAATCCG+AGG | 0.593000 | 1.4:+28363800 | None:intergenic |
| ATTGGAAAGAAAATCTTGAT+GGG | 0.593898 | 1.4:-28363223 | MS.gene053418:CDS |
| TTTCCAATCATGGGCAAGAG+GGG | 0.599297 | 1.4:+28363238 | None:intergenic |
| TCTTTCCAATCATGGGCAAG+AGG | 0.602666 | 1.4:+28363236 | None:intergenic |
| CATCTGATATAGATCATCCC+TGG | 0.602981 | 1.4:+28363334 | None:intergenic |
| GAGTTTGTATAGTGAATTGG+AGG | 0.605286 | 1.4:-28364233 | MS.gene053418:CDS |
| CTATCCTCATTTAGTTGGCG+AGG | 0.607619 | 1.4:-28363360 | MS.gene053418:CDS |
| CCTGTCGTGCAGAGAAGGGG+AGG | 0.610127 | 1.4:-28363836 | MS.gene053418:CDS |
| CTCCTCGGTCTTCTCACGGT+CGG | 0.611073 | 1.4:+28363079 | None:intergenic |
| AAAACTATTGTTCCTCAAAG+CGG | 0.613283 | 1.4:+28363994 | None:intergenic |
| AGATTTGATGATGTCGACGA+CGG | 0.613968 | 1.4:-28363677 | MS.gene053418:CDS |
| CTCCCCGGACTTGTTCCGCA+AGG | 0.617582 | 1.4:+28363740 | None:intergenic |
| GAGGAGCTACTAGTAAGCCT+CGG | 0.623895 | 1.4:-28363817 | MS.gene053418:CDS |
| CCTCGGATTGACATGAGAAG+TGG | 0.624882 | 1.4:-28363800 | MS.gene053418:CDS |
| TTCCTTGCGGAACAAGTCCG+GGG | 0.632704 | 1.4:-28363742 | MS.gene053418:CDS |
| GTTCTGAGCCTGCTTTACAA+GGG | 0.634634 | 1.4:-28364375 | MS.gene053418:CDS |
| AGCATATGGAAGGAATGGTG+AGG | 0.646265 | 1.4:-28364285 | MS.gene053418:CDS |
| GAGCCACCAAGTGTTGAAGG+AGG | 0.655057 | 1.4:+28363437 | None:intergenic |
| GTCCTAAGTCATGTTCAAAG+TGG | 0.658478 | 1.4:-28363551 | MS.gene053418:CDS |
| ATATGTAATTCAACCCAACA+AGG | 0.673128 | 1.4:+28363042 | None:intergenic |
| TAGAAACAACACTAGCAACA+CGG | 0.673276 | 1.4:+28364413 | None:intergenic |
| CGGTTATGTTATCAATCACG+AGG | 0.683171 | 1.4:-28363657 | MS.gene053418:CDS |
| CGCCGACCGTGAGAAGACCG+AGG | 0.715006 | 1.4:-28363081 | MS.gene053418:CDS |
| CATGTATCTAGCAGTCACAG+TGG | 0.766775 | 1.4:-28363623 | MS.gene053418:CDS |
| ATGGAGTGGCAATTACAATG+TGG | 0.799944 | 1.4:-28362954 | MS.gene053418:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTGATTTTGAAGATTGTA+TGG | - | chr1.4:28364692-28364711 | MS.gene053418:CDS | 20.0% |
| ! | ACAGAGAATAAAGAAACTAT+AGG | - | chr1.4:28363043-28363062 | MS.gene053418:CDS | 25.0% |
| ! | ATTGGAAAGAAAATCTTGAT+GGG | - | chr1.4:28364442-28364461 | MS.gene053418:CDS | 25.0% |
| ! | TATGAGTTTGTATAGTGAAT+TGG | - | chr1.4:28363429-28363448 | MS.gene053418:CDS | 25.0% |
| !! | AAGATTTTCTTTCCAATCAT+GGG | + | chr1.4:28364439-28364458 | None:intergenic | 25.0% |
| !!! | AATGAATGTGGATTTTTTTC+TGG | - | chr1.4:28363718-28363737 | MS.gene053418:CDS | 25.0% |
| AAAACTATTGTTCCTCAAAG+CGG | + | chr1.4:28363674-28363693 | None:intergenic | 30.0% | |
| AGAAGCTTGGATTGTAAAAA+TGG | - | chr1.4:28362943-28362962 | MS.gene053418:CDS | 30.0% | |
| ATATGTAATTCAACCCAACA+AGG | + | chr1.4:28364626-28364645 | None:intergenic | 30.0% | |
| ATTAATGTTCAGACGAATTC+AGG | - | chr1.4:28363745-28363764 | MS.gene053418:CDS | 30.0% | |
| ATTTGAAGGATGTTTCTCTT+TGG | - | chr1.4:28363563-28363582 | MS.gene053418:CDS | 30.0% | |
| CATGTTTATAGTGACATTGT+TGG | - | chr1.4:28363319-28363338 | MS.gene053418:CDS | 30.0% | |
| CTCTATGACAATTATGACAT+TGG | + | chr1.4:28364274-28364293 | None:intergenic | 30.0% | |
| GAAGCTTGGATTGTAAAAAT+GGG | - | chr1.4:28362944-28362963 | MS.gene053418:CDS | 30.0% | |
| GAAGTTGTATGTCAAGAATT+TGG | - | chr1.4:28364392-28364411 | MS.gene053418:CDS | 30.0% | |
| GATTGGAAAGAAAATCTTGA+TGG | - | chr1.4:28364441-28364460 | MS.gene053418:CDS | 30.0% | |
| GTTTCAGATTTGAACTTACT+TGG | + | chr1.4:28362973-28362992 | None:intergenic | 30.0% | |
| TTAATGTTCAGACGAATTCA+GGG | - | chr1.4:28363746-28363765 | MS.gene053418:CDS | 30.0% | |
| TTATTGATGTGAAGGAGTTA+GGG | - | chr1.4:28363347-28363366 | MS.gene053418:CDS | 30.0% | |
| TTGCACATAATATGATGAGA+TGG | - | chr1.4:28364487-28364506 | MS.gene053418:CDS | 30.0% | |
| TTGGAATCAGACATTTGATA+AGG | - | chr1.4:28363582-28363601 | MS.gene053418:CDS | 30.0% | |
| ! | CAAGATTTTCTTTCCAATCA+TGG | + | chr1.4:28364440-28364459 | None:intergenic | 30.0% |
| ! | CTTTTGAGCAGAAACTTTTA+TGG | - | chr1.4:28363521-28363540 | MS.gene053418:CDS | 30.0% |
| !! | ATTTTGAAGATTGTATGGAG+TGG | - | chr1.4:28364697-28364716 | MS.gene053418:CDS | 30.0% |
| !!! | TTTTTGGTTAAGCATATGGA+AGG | - | chr1.4:28363370-28363389 | MS.gene053418:CDS | 30.0% |
| AAAATCTTGATGGGATGCTT+CGG | - | chr1.4:28364451-28364470 | MS.gene053418:CDS | 35.0% | |
| AATGAGATCTTGAACACTGA+AGG | - | chr1.4:28363154-28363173 | MS.gene053418:CDS | 35.0% | |
| ACAACAGCAAAATGCATTGT+TGG | - | chr1.4:28364656-28364675 | MS.gene053418:CDS | 35.0% | |
| AGAACTAAGACAAGCCAAAT+CGG | - | chr1.4:28364152-28364171 | MS.gene053418:CDS | 35.0% | |
| CTCATCATATTATGTGCAAG+AGG | + | chr1.4:28364486-28364505 | None:intergenic | 35.0% | |
| GAGTTTGTATAGTGAATTGG+AGG | - | chr1.4:28363432-28363451 | MS.gene053418:CDS | 35.0% | |
| GCTAGAATTTCTGTGATCTT+CGG | - | chr1.4:28363643-28363662 | MS.gene053418:CDS | 35.0% | |
| GTTATTGATGTGAAGGAGTT+AGG | - | chr1.4:28363346-28363365 | MS.gene053418:CDS | 35.0% | |
| GTTTATAGTGACATTGTTGG+TGG | - | chr1.4:28363322-28363341 | MS.gene053418:CDS | 35.0% | |
| TAGAAACAACACTAGCAACA+CGG | + | chr1.4:28363255-28363274 | None:intergenic | 35.0% | |
| ! | AATTTGGTTTCGTCTGATGA+AGG | - | chr1.4:28363184-28363203 | MS.gene053418:CDS | 35.0% |
| ! | CCACATTCATTTTGCATAAC+CGG | + | chr1.4:28363709-28363728 | None:intergenic | 35.0% |
| ! | TGATAAGGTTGTTGAGTTGT+TGG | - | chr1.4:28363597-28363616 | MS.gene053418:CDS | 35.0% |
| !! | CACTGAAGGTGTTAAGAATT+TGG | - | chr1.4:28363168-28363187 | MS.gene053418:CDS | 35.0% |
| !! | TAAACGGCTAACCTACTTTT+CGG | + | chr1.4:28364204-28364223 | None:intergenic | 35.0% |
| !! | TTATCTTCTTGAGCTTGCTA+TGG | - | chr1.4:28363207-28363226 | MS.gene053418:CDS | 35.0% |
| !!! | AGGGTTTTTGGTTAAGCATA+TGG | - | chr1.4:28363366-28363385 | MS.gene053418:CDS | 35.0% |
| !!! | GCTTTGAGGAACAATAGTTT+TGG | - | chr1.4:28363673-28363692 | MS.gene053418:CDS | 35.0% |
| !!! | TTGAGGAACAATAGTTTTGG+TGG | - | chr1.4:28363676-28363695 | MS.gene053418:CDS | 35.0% |
| AAGAGAAATGGATCTCATCC+GGG | - | chr1.4:28363790-28363809 | MS.gene053418:CDS | 40.0% | |
| AAGCCTAAGGTTATCTCTCA+AGG | - | chr1.4:28364362-28364381 | MS.gene053418:CDS | 40.0% | |
| AGAGATAACCTTAGGCTTGT+TGG | + | chr1.4:28364360-28364379 | None:intergenic | 40.0% | |
| ATGGAGTGGCAATTACAATG+TGG | - | chr1.4:28364711-28364730 | MS.gene053418:CDS | 40.0% | |
| CAACGTTTAGTCCGAAAAGT+AGG | - | chr1.4:28364190-28364209 | MS.gene053418:CDS | 40.0% | |
| CAAGAGAAATGGATCTCATC+CGG | - | chr1.4:28363789-28363808 | MS.gene053418:CDS | 40.0% | |
| CAAGGAAAACCAAAATCCTC+AGG | + | chr1.4:28363910-28363929 | None:intergenic | 40.0% | |
| CATCTGATATAGATCATCCC+TGG | + | chr1.4:28364334-28364353 | None:intergenic | 40.0% | |
| CATTTGTCCTGCTAACTATC+TGG | + | chr1.4:28364539-28364558 | None:intergenic | 40.0% | |
| CCGGTTATGCAAAATGAATG+TGG | - | chr1.4:28363706-28363725 | MS.gene053418:CDS | 40.0% | |
| CGGTTATGTTATCAATCACG+AGG | - | chr1.4:28364008-28364027 | MS.gene053418:CDS | 40.0% | |
| CTTCGCTATCCTCATTTAGT+TGG | - | chr1.4:28364300-28364319 | MS.gene053418:CDS | 40.0% | |
| GAACTAAGACAAGCCAAATC+GGG | - | chr1.4:28364153-28364172 | MS.gene053418:CDS | 40.0% | |
| GAATGGTGAGGAAAATGGAT+AGG | - | chr1.4:28363392-28363411 | MS.gene053418:CDS | 40.0% | |
| GCAAGATGTGAGACATTTGA+AGG | - | chr1.4:28363549-28363568 | MS.gene053418:CDS | 40.0% | |
| GGTTAAGCATATGGAAGGAA+TGG | - | chr1.4:28363375-28363394 | MS.gene053418:CDS | 40.0% | |
| GTCCTAAGTCATGTTCAAAG+TGG | - | chr1.4:28364114-28364133 | MS.gene053418:CDS | 40.0% | |
| TGAGATCCATTTCTCTTGCT+TGG | + | chr1.4:28363787-28363806 | None:intergenic | 40.0% | |
| TGCGAATAGTAGCGTGAAAA+GGG | - | chr1.4:28364071-28364090 | MS.gene053418:CDS | 40.0% | |
| TTGCGAATAGTAGCGTGAAA+AGG | - | chr1.4:28364070-28364089 | MS.gene053418:CDS | 40.0% | |
| ! | AGCAGAAACTTTTATGGCAG+AGG | - | chr1.4:28363527-28363546 | MS.gene053418:CDS | 40.0% |
| ! | TGTGAAGGAGTTAGGGTTTT+TGG | - | chr1.4:28363354-28363373 | MS.gene053418:CDS | 40.0% |
| ! | TGTTGAAGGAGGAGCATAAA+CGG | + | chr1.4:28364220-28364239 | None:intergenic | 40.0% |
| ! | TTTCAGAGTCTGAGATCTCA+AGG | - | chr1.4:28363125-28363144 | MS.gene053418:CDS | 40.0% |
| !! | AGATTTGATGATGTCGACGA+CGG | - | chr1.4:28363988-28364007 | MS.gene053418:CDS | 40.0% |
| !! | ATTGGAGGTGTTGAATGAGT+TGG | - | chr1.4:28363447-28363466 | MS.gene053418:CDS | 40.0% |
| !! | GCTAGTGTTGTTTCTAGGTT+AGG | - | chr1.4:28363259-28363278 | MS.gene053418:CDS | 40.0% |
| !! | GTGTTGCTAGTGTTGTTTCT+AGG | - | chr1.4:28363254-28363273 | MS.gene053418:CDS | 40.0% |
| !! | TGGTGGTGTTATTGATGTGA+AGG | - | chr1.4:28363339-28363358 | MS.gene053418:CDS | 40.0% |
| !! | TTTGGCTTGTCTTAGTTCTC+TGG | + | chr1.4:28364151-28364170 | None:intergenic | 40.0% |
| !!! | AGGAACAATAGTTTTGGTGG+TGG | - | chr1.4:28363679-28363698 | MS.gene053418:CDS | 40.0% |
| !!! | GAGGATTTTGGTTTTCCTTG+CGG | - | chr1.4:28363910-28363929 | MS.gene053418:CDS | 40.0% |
| AGATGCTACCAACAAGCCTA+AGG | - | chr1.4:28364349-28364368 | MS.gene053418:CDS | 45.0% | |
| AGCATATGGAAGGAATGGTG+AGG | - | chr1.4:28363380-28363399 | MS.gene053418:CDS | 45.0% | |
| CACTCCATAAAAAGTCTCCC+CGG | + | chr1.4:28363943-28363962 | None:intergenic | 45.0% | |
| CATGACTTAGGACACCTGAA+TGG | + | chr1.4:28364107-28364126 | None:intergenic | 45.0% | |
| CATGTATCTAGCAGTCACAG+TGG | - | chr1.4:28364042-28364061 | MS.gene053418:CDS | 45.0% | |
| CGATTTGCGATCTCCTTGTT+GGG | - | chr1.4:28364610-28364629 | MS.gene053418:CDS | 45.0% | |
| CGCAATCCAAGCAAGAGAAA+TGG | - | chr1.4:28363778-28363797 | MS.gene053418:CDS | 45.0% | |
| CTATCCTCATTTAGTTGGCG+AGG | - | chr1.4:28364305-28364324 | MS.gene053418:CDS | 45.0% | |
| CTTTCCAATCATGGGCAAGA+GGG | + | chr1.4:28364431-28364450 | None:intergenic | 45.0% | |
| GGAAGGAATGGTGAGGAAAA+TGG | - | chr1.4:28363387-28363406 | MS.gene053418:CDS | 45.0% | |
| TATGCTCCTCCTTCAACACT+TGG | - | chr1.4:28364222-28364241 | MS.gene053418:CDS | 45.0% | |
| TCGCCTTGAGAGATAACCTT+AGG | + | chr1.4:28364368-28364387 | None:intergenic | 45.0% | |
| TCTTTCCAATCATGGGCAAG+AGG | + | chr1.4:28364432-28364451 | None:intergenic | 45.0% | |
| TGCTCAAACCCTTGTAAAGC+AGG | + | chr1.4:28363301-28363320 | None:intergenic | 45.0% | |
| TTTCCAATCATGGGCAAGAG+GGG | + | chr1.4:28364430-28364449 | None:intergenic | 45.0% | |
| ! | AAGTCCGGGGAGACTTTTTA+TGG | - | chr1.4:28363936-28363955 | MS.gene053418:CDS | 45.0% |
| ! | AGGTTGTTGAGTTGTTGGCT+AGG | - | chr1.4:28363602-28363621 | MS.gene053418:CDS | 45.0% |
| ! | CGCCACTTTGAACATGACTT+AGG | + | chr1.4:28364119-28364138 | None:intergenic | 45.0% |
| ! | GTGGCGAATTAGCTTCTCTT+CGG | - | chr1.4:28363884-28363903 | MS.gene053418:CDS | 45.0% |
| ! | GTTCTGAGCCTGCTTTACAA+GGG | - | chr1.4:28363290-28363309 | MS.gene053418:CDS | 45.0% |
| ! | TCGATTTGCGATCTCCTTGT+TGG | - | chr1.4:28364609-28364628 | MS.gene053418:CDS | 45.0% |
| ! | TGTTCTGAGCCTGCTTTACA+AGG | - | chr1.4:28363289-28363308 | MS.gene053418:CDS | 45.0% |
| ! | TTTTCCTTGCGGAACAAGTC+CGG | - | chr1.4:28363921-28363940 | MS.gene053418:CDS | 45.0% |
| !! | AGGGAGCATTTGTTCCATTC+AGG | - | chr1.4:28364090-28364109 | MS.gene053418:CDS | 45.0% |
| AATGCCTGTCGTGCAGAGAA+GGG | - | chr1.4:28363825-28363844 | MS.gene053418:CDS | 50.0% | |
| AGCAGCACCAGATAGTTAGC+AGG | - | chr1.4:28364529-28364548 | MS.gene053418:CDS | 50.0% | |
| ATCTTGATGGGATGCTTCGG+TGG | - | chr1.4:28364454-28364473 | MS.gene053418:CDS | 50.0% | |
| ATTTAGTTGGCGAGGAAGCC+AGG | - | chr1.4:28364313-28364332 | MS.gene053418:CDS | 50.0% | |
| CCACTTCTCATGTCAATCCG+AGG | + | chr1.4:28363868-28363887 | None:intergenic | 50.0% | |
| CCTCGGATTGACATGAGAAG+TGG | - | chr1.4:28363865-28363884 | MS.gene053418:CDS | 50.0% | |
| GAGGAGCTACTAGTAAGCCT+CGG | - | chr1.4:28363848-28363867 | MS.gene053418:CDS | 50.0% | |
| GCAACTTTGTACGCCCGATT+TGG | + | chr1.4:28364169-28364188 | None:intergenic | 50.0% | |
| GCTTCCTCGCCAACTAAATG+AGG | + | chr1.4:28364312-28364331 | None:intergenic | 50.0% | |
| GGCATTCTTGCAATCGAACC+CGG | + | chr1.4:28363811-28363830 | None:intergenic | 50.0% | |
| TCTTCGGTGAAACCGCTTTG+AGG | - | chr1.4:28363659-28363678 | MS.gene053418:CDS | 50.0% | |
| TTTAGTTGGCGAGGAAGCCA+GGG | - | chr1.4:28364314-28364333 | MS.gene053418:CDS | 50.0% | |
| TTTCCTTGCGGAACAAGTCC+GGG | - | chr1.4:28363922-28363941 | MS.gene053418:CDS | 50.0% | |
| ! | ATTAGCTTCTCTTCGGCCTG+AGG | - | chr1.4:28363891-28363910 | MS.gene053418:CDS | 50.0% |
| ! | GAGATCGCAAATCGACTCCT+CGG | + | chr1.4:28364604-28364623 | None:intergenic | 50.0% |
| ! | TCTCTTCGGCCTGAGGATTT+TGG | - | chr1.4:28363898-28363917 | MS.gene053418:CDS | 50.0% |
| !!! | TAGTTTTGGTGGTGGTTCGC+CGG | - | chr1.4:28363687-28363706 | MS.gene053418:CDS | 50.0% |
| ATGCCTGTCGTGCAGAGAAG+GGG | - | chr1.4:28363826-28363845 | MS.gene053418:CDS | 55.0% | |
| GAATGCCTGTCGTGCAGAGA+AGG | - | chr1.4:28363824-28363843 | MS.gene053418:CDS | 55.0% | |
| GCTCCTCCTTCAACACTTGG+TGG | - | chr1.4:28364225-28364244 | MS.gene053418:CDS | 55.0% | |
| TCGACTCCTCGGTCTTCTCA+CGG | + | chr1.4:28364593-28364612 | None:intergenic | 55.0% | |
| TTCCTTGCGGAACAAGTCCG+GGG | - | chr1.4:28363923-28363942 | MS.gene053418:CDS | 55.0% | |
| !! | GAGCCACCAAGTGTTGAAGG+AGG | + | chr1.4:28364231-28364250 | None:intergenic | 55.0% |
| !! | GCTGAGCCACCAAGTGTTGA+AGG | + | chr1.4:28364234-28364253 | None:intergenic | 55.0% |
| !! | GGCAAGAGGGGCGTCATATA+TGG | + | chr1.4:28364418-28364437 | None:intergenic | 55.0% |
| ACGCCCCTCTTGCCCATGAT+TGG | - | chr1.4:28364424-28364443 | MS.gene053418:CDS | 60.0% | |
| CTCCTCGGTCTTCTCACGGT+CGG | + | chr1.4:28364589-28364608 | None:intergenic | 60.0% | |
| CCTCCCCTTCTCTGCACGAC+AGG | + | chr1.4:28363832-28363851 | None:intergenic | 65.0% | |
| CCTGTCGTGCAGAGAAGGGG+AGG | - | chr1.4:28363829-28363848 | MS.gene053418:CDS | 65.0% | |
| ! | CTCCCCGGACTTGTTCCGCA+AGG | + | chr1.4:28363928-28363947 | None:intergenic | 65.0% |
| CGCCGACCGTGAGAAGACCG+AGG | - | chr1.4:28364584-28364603 | MS.gene053418:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 28362935 | 28364752 | 28362935 | ID=MS.gene053418 |
| chr1.4 | mRNA | 28362935 | 28364752 | 28362935 | ID=MS.gene053418.t1;Parent=MS.gene053418 |
| chr1.4 | exon | 28362935 | 28364752 | 28362935 | ID=MS.gene053418.t1.exon1;Parent=MS.gene053418.t1 |
| chr1.4 | CDS | 28362935 | 28364752 | 28362935 | ID=cds.MS.gene053418.t1;Parent=MS.gene053418.t1 |
| Gene Sequence |
| Protein sequence |
