Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65894.t1 | RHN55952.1 | 92.4 | 410 | 30 | 1 | 5 | 414 | 3 | 411 | 1.10E-219 | 772.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65894.t1 | Q99JW2 | 42.4 | 387 | 207 | 6 | 30 | 414 | 5 | 377 | 2.8e-86 | 320.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65894.t1 | A0A396HRI7 | 92.4 | 410 | 30 | 1 | 5 | 414 | 3 | 411 | 8.1e-220 | 772.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene65894 | MS.gene29220 | PPI |
| MS.gene65894 | MS.gene91122 | PPI |
| MS.gene65894 | MS.gene91126 | PPI |
| MS.gene026174 | MS.gene65894 | PPI |
| MS.gene88610 | MS.gene65894 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65894.t1 | MTR_5g058430 | 95.666 | 323 | 14 | 0 | 92 | 414 | 4 | 326 | 0.0 | 646 |
| MS.gene65894.t1 | MTR_1g039010 | 54.569 | 383 | 173 | 1 | 32 | 414 | 28 | 409 | 7.31e-156 | 447 |
| MS.gene65894.t1 | MTR_1g038920 | 54.497 | 378 | 171 | 1 | 37 | 414 | 36 | 412 | 4.22e-153 | 441 |
| MS.gene65894.t1 | MTR_1g038920 | 54.068 | 381 | 171 | 2 | 37 | 414 | 36 | 415 | 2.70e-151 | 436 |
| MS.gene65894.t1 | MTR_0400s0010 | 55.932 | 177 | 75 | 1 | 241 | 414 | 1 | 177 | 2.03e-70 | 220 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65894.t1 | AT1G44820 | 67.246 | 403 | 129 | 2 | 12 | 414 | 7 | 406 | 0.0 | 584 |
| MS.gene65894.t1 | AT1G44180 | 66.334 | 401 | 134 | 1 | 14 | 414 | 9 | 408 | 0.0 | 580 |
| MS.gene65894.t1 | AT1G44180 | 66.334 | 401 | 134 | 1 | 14 | 414 | 9 | 408 | 0.0 | 580 |
| MS.gene65894.t1 | AT1G44180 | 66.334 | 401 | 134 | 1 | 14 | 414 | 9 | 408 | 0.0 | 580 |
| MS.gene65894.t1 | AT4G38220 | 54.450 | 382 | 168 | 2 | 33 | 414 | 21 | 396 | 2.90e-156 | 448 |
| MS.gene65894.t1 | AT4G38220 | 54.569 | 383 | 169 | 3 | 33 | 414 | 21 | 399 | 3.83e-156 | 448 |
Find 135 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTCCTTCTCTTTACATGGCC+TGG | 0.205801 | 5.2:+49608862 | MS.gene65894:CDS |
| CTTCGGTTGGTGATGAGTTT+AGG | 0.214559 | 5.2:+49609217 | MS.gene65894:CDS |
| AGGACCCATCAGAGACTATT+TGG | 0.216846 | 5.2:+49615761 | MS.gene65894:CDS |
| GTTGTGGATTGAAGAATTTC+AGG | 0.246803 | 5.2:-49615876 | None:intergenic |
| TCATTAGTTGCAGTCATTAA+AGG | 0.282446 | 5.2:-49615789 | None:intergenic |
| TTCGGTTGGTGATGAGTTTA+GGG | 0.303395 | 5.2:+49609218 | MS.gene65894:CDS |
| TCCTTCTCTTTACATGGCCT+GGG | 0.318452 | 5.2:+49608863 | MS.gene65894:CDS |
| GCGGAGGTGTAATCTGGATT+TGG | 0.322659 | 5.2:-49608760 | None:intergenic |
| GATGAGGAAGTTGGTGGATT+TGG | 0.346416 | 5.2:+49609120 | MS.gene65894:CDS |
| ACTCCAGCCTTAACATAAAC+TGG | 0.359930 | 5.2:-49609438 | None:intergenic |
| GTTGAATTCCGAGGTTGTTT+CGG | 0.366528 | 5.2:+49609410 | MS.gene65894:CDS |
| GAAATAGCGGAGGTGTAATC+TGG | 0.382097 | 5.2:-49608766 | None:intergenic |
| GGAAGGGATGGAGTTGGATT+GGG | 0.382893 | 5.2:-49608800 | None:intergenic |
| CAGTTTGATGTTGTTAAGGC+TGG | 0.384823 | 5.2:+49609381 | MS.gene65894:CDS |
| TCCCTGCTGAACCTTCTAAA+TGG | 0.394541 | 5.2:+49608935 | MS.gene65894:CDS |
| GGACCCATCAGAGACTATTT+GGG | 0.396497 | 5.2:+49615762 | MS.gene65894:CDS |
| ATTCTCCTCTTCATTTCGTC+AGG | 0.396666 | 5.2:-49609850 | None:intergenic |
| TGGTGGTTGGCAGTTGTTTG+TGG | 0.402438 | 5.2:+49609140 | MS.gene65894:CDS |
| AGCCTCTCCTTCTCTTTACA+TGG | 0.413508 | 5.2:+49608857 | MS.gene65894:CDS |
| CGGAGGTGTAATCTGGATTT+GGG | 0.414688 | 5.2:-49608759 | None:intergenic |
| GAGTCAGTTTGATGTTGTTA+AGG | 0.415690 | 5.2:+49609377 | MS.gene65894:CDS |
| CAGAAAGGGATTCCTGTACT+TGG | 0.416073 | 5.2:+49615918 | MS.gene65894:CDS |
| GGAGAATGGAAGGGAGAGAA+GGG | 0.423705 | 5.2:-49608891 | None:intergenic |
| GGAGGATGGAGCCATTTAGA+AGG | 0.426494 | 5.2:-49608946 | None:intergenic |
| GCTTCCCTGGTGAGAATTCA+AGG | 0.428504 | 5.2:-49608837 | None:intergenic |
| GATTAAAGCGAAAGGGCAAC+CGG | 0.433872 | 5.2:+49609275 | MS.gene65894:CDS |
| AGGTTGTTGTTGTTGAGAGA+AGG | 0.435970 | 5.2:-49608669 | None:intergenic |
| CGCATCGCGAACAGCGGAGA+AGG | 0.437222 | 5.2:-49608968 | None:intergenic |
| TGCAATACCTCGAAGCAATT+CGG | 0.438262 | 5.2:+49609040 | MS.gene65894:CDS |
| TTGTGGTCATGAAGCAAGAT+AGG | 0.439411 | 5.2:-49615963 | None:intergenic |
| GGTTAATCCAGTTTATGTTA+AGG | 0.441495 | 5.2:+49609431 | MS.gene65894:CDS |
| AAGTTGGTGGATTTGGTGGT+TGG | 0.442213 | 5.2:+49609127 | MS.gene65894:CDS |
| AGGAGAATGGAAGGGAGAGA+AGG | 0.443082 | 5.2:-49608892 | None:intergenic |
| GAGGAAGTTGGTGGATTTGG+TGG | 0.443100 | 5.2:+49609123 | MS.gene65894:CDS |
| GAATGGAAGGGAGAGAAGGG+TGG | 0.443594 | 5.2:-49608888 | None:intergenic |
| TGGAAGGGATGGAGTTGGAT+TGG | 0.447611 | 5.2:-49608801 | None:intergenic |
| GGGAGAGAAGGGTGGGACCC+AGG | 0.448707 | 5.2:-49608880 | None:intergenic |
| CAACTTCCTCATCAGGAACT+AGG | 0.449328 | 5.2:-49609110 | None:intergenic |
| GAGAAGTGTGAGTGAGAGAA+TGG | 0.450138 | 5.2:-49608641 | None:intergenic |
| GTTGGATTTGCGTTAGATGA+AGG | 0.463575 | 5.2:+49609189 | MS.gene65894:CDS |
| CAACCTTCGGAGGCAGAAGC+TGG | 0.469578 | 5.2:+49609796 | MS.gene65894:CDS |
| GAATTTGAAAAGATGAATGT+TGG | 0.470089 | 5.2:+49609171 | MS.gene65894:CDS |
| GTTGGATTGGGAGGTGAGGT+AGG | 0.470149 | 5.2:-49608788 | None:intergenic |
| GAGATATGAACCGTCCTAAC+CGG | 0.472738 | 5.2:-49609087 | None:intergenic |
| TGGGAATTGAGGAGAATGGA+AGG | 0.475415 | 5.2:-49608901 | None:intergenic |
| AATCCAGTTTATGTTAAGGC+TGG | 0.478162 | 5.2:+49609435 | MS.gene65894:CDS |
| GTGCACCACGGGCGAAGATA+AGG | 0.479507 | 5.2:-49608996 | None:intergenic |
| GCTTTAATCTTCAAATGCCA+TGG | 0.489025 | 5.2:-49609261 | None:intergenic |
| TATCTTCGCCCGTGGTGCAC+AGG | 0.489257 | 5.2:+49608999 | MS.gene65894:CDS |
| AATCCACCAACTTCCTCATC+AGG | 0.490507 | 5.2:-49609117 | None:intergenic |
| TAGCGTTGGAAACGACTGAT+TGG | 0.490663 | 5.2:-49608715 | None:intergenic |
| TGTGATGAATGTGCAACCTT+CGG | 0.491674 | 5.2:+49609783 | MS.gene65894:CDS |
| CTAGTTCCTGATGAGGAAGT+TGG | 0.492909 | 5.2:+49609111 | MS.gene65894:CDS |
| AGCCATTTAGAAGGTTCAGC+AGG | 0.495114 | 5.2:-49608937 | None:intergenic |
| AATATATCGAGCATCGGTTG+TGG | 0.496361 | 5.2:-49615892 | None:intergenic |
| TTTGTGATGGATGGAAGGGA+TGG | 0.496852 | 5.2:-49608812 | None:intergenic |
| TTTATCATCCTGTGCACCAC+GGG | 0.498318 | 5.2:-49609007 | None:intergenic |
| CAGAATCAAGATGGGAATTG+AGG | 0.498462 | 5.2:-49608912 | None:intergenic |
| ATGGATGGAAGGGATGGAGT+TGG | 0.501034 | 5.2:-49608806 | None:intergenic |
| GTTAGATGAAGGACAAGCTT+CGG | 0.501465 | 5.2:+49609200 | MS.gene65894:CDS |
| GAAAACTAACCACCATGGAT+TGG | 0.501858 | 5.2:-49615814 | None:intergenic |
| GAGGAGAATTGCTGCTGAAT+GGG | 0.503044 | 5.2:+49609864 | MS.gene65894:CDS |
| GCTCGATATATTCGACAGAA+AGG | 0.510109 | 5.2:+49615903 | MS.gene65894:CDS |
| TGTTAGAAATATGTCATATG+AGG | 0.518886 | 5.2:+49609894 | MS.gene65894:CDS |
| TACAATCTCGAACCATGCCC+CGG | 0.520996 | 5.2:-49609294 | None:intergenic |
| GGAGGTGAGGTAGGAAATAG+CGG | 0.521837 | 5.2:-49608779 | None:intergenic |
| AGAGGAGAATTGCTGCTGAA+TGG | 0.522314 | 5.2:+49609863 | MS.gene65894:CDS |
| TAAGATTCCGAATTGCTTCG+AGG | 0.522352 | 5.2:-49609047 | None:intergenic |
| TGTTTATGCGAAGGTAGCGT+TGG | 0.524429 | 5.2:-49608729 | None:intergenic |
| AAGCAGGCTATAGCCTCAGC+TGG | 0.528719 | 5.2:+49615837 | MS.gene65894:CDS |
| AAGAGAAGGAGAGGCTTCCC+TGG | 0.528914 | 5.2:-49608850 | None:intergenic |
| GTTCCTGATGAGGAAGTTGG+TGG | 0.535928 | 5.2:+49609114 | MS.gene65894:CDS |
| CTCGATATATTCGACAGAAA+GGG | 0.537271 | 5.2:+49615904 | MS.gene65894:CDS |
| TCCGCTGTTCGCGATGCGGA+CGG | 0.538698 | 5.2:+49608973 | MS.gene65894:CDS |
| CAGCAGGGACAGAATCAAGA+TGG | 0.540176 | 5.2:-49608921 | None:intergenic |
| TTTATGCGGATCGGTTTCCA+TGG | 0.541080 | 5.2:+49609244 | MS.gene65894:CDS |
| TGATGTTGTTAAGGCTGGGA+AGG | 0.542641 | 5.2:+49609386 | MS.gene65894:CDS |
| AAAACCCTTGAATTCTCACC+AGG | 0.543917 | 5.2:+49608832 | MS.gene65894:CDS |
| ATTTGAAGATTAAAGCGAAA+GGG | 0.543919 | 5.2:+49609268 | MS.gene65894:CDS |
| ACGCCCCAAATAGTCTCTGA+TGG | 0.548595 | 5.2:-49615766 | None:intergenic |
| AAGATGGGAATTGAGGAGAA+TGG | 0.548911 | 5.2:-49608905 | None:intergenic |
| GCAGTTGTTTGTGGAGTCTG+AGG | 0.551332 | 5.2:+49609149 | MS.gene65894:CDS |
| ATTAAAGCGAAAGGGCAACC+GGG | 0.555182 | 5.2:+49609276 | MS.gene65894:CDS |
| AACTTCCTCATCAGGAACTA+GGG | 0.558763 | 5.2:-49609109 | None:intergenic |
| AATGGAAGGGAGAGAAGGGT+GGG | 0.559746 | 5.2:-49608887 | None:intergenic |
| GAACAGCGGAGAAGGGAGGA+TGG | 0.561070 | 5.2:-49608960 | None:intergenic |
| ATTGGGGAGAAACCAAGTAC+AGG | 0.562420 | 5.2:-49615930 | None:intergenic |
| CTTCCCTGGTGAGAATTCAA+GGG | 0.569218 | 5.2:-49608836 | None:intergenic |
| AAACCAGCTTCTGCCTCCGA+AGG | 0.573740 | 5.2:-49609799 | None:intergenic |
| GCGAAAGGGCAACCGGGGCA+TGG | 0.576892 | 5.2:+49609282 | MS.gene65894:CDS |
| GGCCATGTAAAGAGAAGGAG+AGG | 0.579297 | 5.2:-49608859 | None:intergenic |
| TGGAGTTGGATTGGGAGGTG+AGG | 0.579366 | 5.2:-49608792 | None:intergenic |
| GATGAAGGACAAGCTTCGGT+TGG | 0.580873 | 5.2:+49609204 | MS.gene65894:CDS |
| CGCCCCAAATAGTCTCTGAT+GGG | 0.582098 | 5.2:-49615765 | None:intergenic |
| CAACTAATGATTCCAATCCA+TGG | 0.582221 | 5.2:+49615802 | MS.gene65894:CDS |
| GGGAATTGAGGAGAATGGAA+GGG | 0.583024 | 5.2:-49608900 | None:intergenic |
| AGTTTGATGTTGTTAAGGCT+GGG | 0.586632 | 5.2:+49609382 | MS.gene65894:CDS |
| GCATCGCGAACAGCGGAGAA+GGG | 0.589130 | 5.2:-49608967 | None:intergenic |
| CTGTCGAATATATCGAGCAT+CGG | 0.589926 | 5.2:-49615898 | None:intergenic |
| ATTTCGTCAGGATCTGTCGT+AGG | 0.590363 | 5.2:-49609838 | None:intergenic |
| CAGATCCTGACGAAATGAAG+AGG | 0.592088 | 5.2:+49609845 | MS.gene65894:CDS |
| CTTCTCCGCTGTTCGCGATG+CGG | 0.593617 | 5.2:+49608969 | MS.gene65894:CDS |
| ATTGTATGATGATAGTGCTA+TGG | 0.595056 | 5.2:+49609311 | MS.gene65894:CDS |
| ATATTGCAGATCATAGAGAA+AGG | 0.596427 | 5.2:+49615741 | MS.gene65894:intron |
| ATTCGGAATCTTAACAACAA+GGG | 0.596614 | 5.2:+49609057 | MS.gene65894:CDS |
| TGCCAAACTCACATCATGAG+TGG | 0.600194 | 5.2:-49609463 | None:intergenic |
| GAATTTGATGAAGAGTGTTG+AGG | 0.601037 | 5.2:+49609335 | MS.gene65894:CDS |
| ACCCAGGCCATGTAAAGAGA+AGG | 0.604148 | 5.2:-49608864 | None:intergenic |
| AGAGTGTTGAGGTTGTGAGT+AGG | 0.605145 | 5.2:+49609346 | MS.gene65894:CDS |
| TGGATTAACCGAAACAACCT+CGG | 0.607325 | 5.2:-49609418 | None:intergenic |
| AGCAGGGACAGAATCAAGAT+GGG | 0.608862 | 5.2:-49608920 | None:intergenic |
| CTTGCTTCATGACCACAATG+AGG | 0.609150 | 5.2:+49615968 | MS.gene65894:CDS |
| TCGCGAACAGCGGAGAAGGG+AGG | 0.610664 | 5.2:-49608964 | None:intergenic |
| AATTCGGAATCTTAACAACA+AGG | 0.615006 | 5.2:+49609056 | MS.gene65894:CDS |
| GCCGTCCGCATCGCGAACAG+CGG | 0.615094 | 5.2:-49608974 | None:intergenic |
| TATGACATATTTCTAACAGA+GGG | 0.617026 | 5.2:-49609889 | None:intergenic |
| GACGGCCTTATCTTCGCCCG+TGG | 0.618435 | 5.2:+49608991 | MS.gene65894:CDS |
| GCCAAACTCACATCATGAGT+GGG | 0.619380 | 5.2:-49609462 | None:intergenic |
| CATTTGAAGATTAAAGCGAA+AGG | 0.620511 | 5.2:+49609267 | MS.gene65894:CDS |
| GCCATTTAGAAGGTTCAGCA+GGG | 0.636542 | 5.2:-49608936 | None:intergenic |
| TGGGAAGGCGTTGAATTCCG+AGG | 0.643000 | 5.2:+49609401 | MS.gene65894:CDS |
| TATCTCCCTAGTTCCTGATG+AGG | 0.655059 | 5.2:+49609104 | MS.gene65894:CDS |
| TTAAAGCGAAAGGGCAACCG+GGG | 0.660611 | 5.2:+49609277 | MS.gene65894:CDS |
| ATTTATCATCCTGTGCACCA+CGG | 0.661983 | 5.2:-49609008 | None:intergenic |
| TGCTTGAAAACTAACCACCA+TGG | 0.670201 | 5.2:-49615819 | None:intergenic |
| AGGGATGGAGTTGGATTGGG+AGG | 0.670881 | 5.2:-49608797 | None:intergenic |
| ATATGACATATTTCTAACAG+AGG | 0.671509 | 5.2:-49609890 | None:intergenic |
| GATGAATGTGCAACCTTCGG+AGG | 0.672443 | 5.2:+49609786 | MS.gene65894:CDS |
| GGTGAGGTAGGAAATAGCGG+AGG | 0.683458 | 5.2:-49608776 | None:intergenic |
| GACCCATCAGAGACTATTTG+GGG | 0.690098 | 5.2:+49615763 | MS.gene65894:CDS |
| GGTGTGCGGTGTTTATGCGA+AGG | 0.690804 | 5.2:-49608738 | None:intergenic |
| GTAATCTGGATTTGGGTGTG+CGG | 0.704552 | 5.2:-49608752 | None:intergenic |
| AAACCCTTGAATTCTCACCA+GGG | 0.729912 | 5.2:+49608833 | MS.gene65894:CDS |
| CTAATGATTCCAATCCATGG+TGG | 0.746783 | 5.2:+49615805 | MS.gene65894:CDS |
| AAAGTTGTGTAACCTCATTG+TGG | 0.788736 | 5.2:-49615980 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.2 | gene | 49608610 | 49615989 | 49608610 | ID=MS.gene65894 |
| chr5.2 | mRNA | 49608610 | 49615989 | 49608610 | ID=MS.gene65894.t1;Parent=MS.gene65894 |
| chr5.2 | exon | 49608610 | 49609473 | 49608610 | ID=MS.gene65894.t1.exon1;Parent=MS.gene65894.t1 |
| chr5.2 | CDS | 49608610 | 49609473 | 49608610 | ID=cds.MS.gene65894.t1;Parent=MS.gene65894.t1 |
| chr5.2 | exon | 49609778 | 49609915 | 49609778 | ID=MS.gene65894.t1.exon2;Parent=MS.gene65894.t1 |
| chr5.2 | CDS | 49609778 | 49609915 | 49609778 | ID=cds.MS.gene65894.t1;Parent=MS.gene65894.t1 |
| chr5.2 | exon | 49615750 | 49615989 | 49615750 | ID=MS.gene65894.t1.exon3;Parent=MS.gene65894.t1 |
| chr5.2 | CDS | 49615750 | 49615989 | 49615750 | ID=cds.MS.gene65894.t1;Parent=MS.gene65894.t1 |
| Gene Sequence |
| Protein sequence |
