Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66273.t1 | XP_003620949.2 | 98.7 | 153 | 2 | 0 | 1 | 153 | 5 | 157 | 9.00E-82 | 312.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66273.t1 | Q8S8N6 | 65.6 | 151 | 45 | 1 | 3 | 153 | 5 | 148 | 2.0e-53 | 209.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66273.t1 | B7FGW1 | 98.7 | 153 | 2 | 0 | 1 | 153 | 5 | 157 | 6.5e-82 | 312.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049298 | MS.gene66273 | 0.807395 | 5.16E-50 | -1.69E-46 |
| MS.gene051439 | MS.gene66273 | 0.802343 | 5.87E-49 | -1.69E-46 |
| MS.gene052169 | MS.gene66273 | 0.869162 | 3.81E-66 | -1.69E-46 |
| MS.gene052209 | MS.gene66273 | 0.808515 | 2.98E-50 | -1.69E-46 |
| MS.gene054662 | MS.gene66273 | 0.826526 | 2.58E-54 | -1.69E-46 |
| MS.gene054941 | MS.gene66273 | 0.821269 | 4.42E-53 | -1.69E-46 |
| MS.gene05652 | MS.gene66273 | 0.801182 | 1.02E-48 | -1.69E-46 |
| MS.gene057367 | MS.gene66273 | 0.810504 | 1.12E-50 | -1.69E-46 |
| MS.gene057988 | MS.gene66273 | 0.81717 | 3.79E-52 | -1.69E-46 |
| MS.gene057990 | MS.gene66273 | 0.818256 | 2.16E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66273.t1 | MTR_6g092790 | 98.693 | 153 | 2 | 0 | 1 | 153 | 5 | 157 | 2.22e-112 | 315 |
| MS.gene66273.t1 | MTR_8g101550 | 62.676 | 142 | 53 | 0 | 8 | 149 | 4 | 145 | 6.67e-65 | 195 |
| MS.gene66273.t1 | MTR_6g091720 | 45.122 | 82 | 35 | 2 | 35 | 116 | 30 | 101 | 9.35e-18 | 75.1 |
| MS.gene66273.t1 | MTR_6g091750 | 36.364 | 110 | 54 | 3 | 42 | 145 | 52 | 151 | 7.45e-17 | 73.2 |
| MS.gene66273.t1 | MTR_6g091700 | 42.697 | 89 | 41 | 2 | 3 | 91 | 1 | 79 | 4.23e-15 | 68.2 |
| MS.gene66273.t1 | MTR_6g091730 | 41.538 | 65 | 36 | 1 | 63 | 127 | 36 | 98 | 3.17e-11 | 57.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66273.t1 | AT2G06925 | 65.563 | 151 | 45 | 1 | 3 | 153 | 5 | 148 | 1.71e-71 | 212 |
| MS.gene66273.t1 | AT2G06925 | 65.306 | 147 | 44 | 1 | 3 | 149 | 5 | 144 | 2.14e-68 | 204 |
| MS.gene66273.t1 | AT2G19690 | 54.839 | 62 | 27 | 1 | 35 | 96 | 23 | 83 | 2.98e-18 | 75.5 |
| MS.gene66273.t1 | AT2G19690 | 46.250 | 80 | 40 | 2 | 35 | 114 | 23 | 99 | 4.63e-18 | 75.9 |
| MS.gene66273.t1 | AT2G19690 | 46.250 | 80 | 40 | 2 | 35 | 114 | 23 | 99 | 5.39e-18 | 75.9 |
| MS.gene66273.t1 | AT4G29460 | 46.914 | 81 | 40 | 2 | 37 | 117 | 23 | 100 | 6.93e-18 | 76.6 |
| MS.gene66273.t1 | AT4G29470 | 42.268 | 97 | 52 | 3 | 21 | 117 | 8 | 100 | 5.65e-17 | 74.3 |
| MS.gene66273.t1 | AT4G29470 | 42.268 | 97 | 52 | 3 | 21 | 117 | 8 | 100 | 5.65e-17 | 74.3 |
Find 21 sgRNAs with CRISPR-Local
Find 73 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGTCATGGAAGCTGCTTTAC+TGG | 0.266457 | 6.4:+64192850 | MS.gene66273:CDS |
| CCATGCAGTCTATGAATGTT+TGG | 0.339978 | 6.4:-64192748 | None:intergenic |
| GTGCCTCCATTGTTGAGATA+TGG | 0.393871 | 6.4:+64191732 | MS.gene66273:intron |
| TGTGAGTCAAGTTTCTGTTC+AGG | 0.441225 | 6.4:+64191638 | MS.gene66273:CDS |
| CATGTGTTTCCTTTGAATGT+AGG | 0.457173 | 6.4:-64192794 | None:intergenic |
| TTTGGCTGCACTGCTGACTT+AGG | 0.479097 | 6.4:-64192730 | None:intergenic |
| CATGTTTATGCACTTAACAT+AGG | 0.509197 | 6.4:+64191470 | MS.gene66273:CDS |
| ATGGAAGCTGCTTTACTGGC+TGG | 0.510582 | 6.4:+64192854 | MS.gene66273:CDS |
| ACAAAGGCTCCTACATTCAA+AGG | 0.530546 | 6.4:+64192785 | MS.gene66273:CDS |
| TACTGTGGACTATTGTATAG+TGG | 0.545854 | 6.4:+64191759 | MS.gene66273:CDS |
| CTTCAATAACATCATCTGCT+TGG | 0.548099 | 6.4:-64192817 | None:intergenic |
| TGAAGTTATCAAGATTGTCA+TGG | 0.550055 | 6.4:+64192835 | MS.gene66273:CDS |
| CCAAACATTCATAGACTGCA+TGG | 0.586577 | 6.4:+64192748 | MS.gene66273:CDS |
| CTATTGTATAGTGGATGCCC+TGG | 0.587699 | 6.4:+64191768 | MS.gene66273:CDS |
| AACATAGGTGCTGAAACCAC+TGG | 0.598513 | 6.4:+64191485 | MS.gene66273:CDS |
| TACTTTCCATATCTCAACAA+TGG | 0.617587 | 6.4:-64191738 | None:intergenic |
| GTTAAGTGCATAAACATGAG+TGG | 0.652688 | 6.4:-64191465 | None:intergenic |
| AACTGAAACAGCTACTCCAG+TGG | 0.671963 | 6.4:-64191501 | None:intergenic |
| TTTCCATATCTCAACAATGG+AGG | 0.673712 | 6.4:-64191735 | None:intergenic |
| TTGAGATATGGAAAGTACTG+TGG | 0.689516 | 6.4:+64191744 | MS.gene66273:CDS |
| CAAGCATCTAGACCATCACA+AGG | 0.698237 | 6.4:-64191798 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AACAATTTAACATATATATT+AGG | + | chr6.4:64192113-64192132 | MS.gene66273:intron | 10.0% |
| !!! | TTAATTAATATGTTTAGAAT+TGG | - | chr6.4:64191552-64191571 | None:intergenic | 10.0% |
| !! | AAATAAAATAAAGATATGGT+AGG | + | chr6.4:64192195-64192214 | MS.gene66273:intron | 15.0% |
| !! | ACACAAATAAAATAAAGATA+TGG | + | chr6.4:64192191-64192210 | MS.gene66273:intron | 15.0% |
| !!! | AAATTCTAAAATATGTTATC+AGG | - | chr6.4:64192046-64192065 | None:intergenic | 15.0% |
| !! | AAATGAAGTAATTAGATGAA+GGG | - | chr6.4:64192329-64192348 | None:intergenic | 20.0% |
| !! | ACTAGTAAATAGTATTAGTT+TGG | - | chr6.4:64192433-64192452 | None:intergenic | 20.0% |
| !! | ATTATCAAATGTAATGGTTA+TGG | - | chr6.4:64192475-64192494 | None:intergenic | 20.0% |
| !! | CATAAGATTAATAGTTACTA+AGG | - | chr6.4:64192539-64192558 | None:intergenic | 20.0% |
| !! | TATGGATAGTCAAAAAATTT+AGG | + | chr6.4:64192017-64192036 | MS.gene66273:intron | 20.0% |
| !! | TTATCAAATGTAATGGTTAT+GGG | - | chr6.4:64192474-64192493 | None:intergenic | 20.0% |
| !!! | ATCTAACATTGTTATTGATT+AGG | + | chr6.4:64191954-64191973 | MS.gene66273:intron | 20.0% |
| !!! | ATTGGTTAGTCTTTATTTAT+GGG | - | chr6.4:64192304-64192323 | None:intergenic | 20.0% |
| !!! | TTTTTAACCAGTATACTAAT+TGG | + | chr6.4:64192251-64192270 | MS.gene66273:intron | 20.0% |
| !!! | TTTTTTGCTATACTTTATGT+TGG | + | chr6.4:64192509-64192528 | MS.gene66273:intron | 20.0% |
| ! | ATTCAATCCTAAAAGCTATA+TGG | + | chr6.4:64191999-64192018 | MS.gene66273:intron | 25.0% |
| ! | GAAATGAAGTAATTAGATGA+AGG | - | chr6.4:64192330-64192349 | None:intergenic | 25.0% |
| ! | GAATCAGATGATACTAAAAA+TGG | - | chr6.4:64191928-64191947 | None:intergenic | 25.0% |
| ! | TAAAATAAAGATATGGTAGG+TGG | + | chr6.4:64192198-64192217 | MS.gene66273:intron | 25.0% |
| ! | TAGCAAAAAAAAATTAGTCC+TGG | - | chr6.4:64192499-64192518 | None:intergenic | 25.0% |
| ! | TATATGTGTCAATATCATGT+TGG | - | chr6.4:64192621-64192640 | None:intergenic | 25.0% |
| ! | TCATCTAATTACTTCATTTC+TGG | + | chr6.4:64192330-64192349 | MS.gene66273:intron | 25.0% |
| !!! | ATAGCTTTTAGGATTGAATT+AGG | - | chr6.4:64191998-64192017 | None:intergenic | 25.0% |
| !!! | CATTGGTTAGTCTTTATTTA+TGG | - | chr6.4:64192305-64192324 | None:intergenic | 25.0% |
| !!! | TTTTTGAAAATTGAGCTGAT+TGG | + | chr6.4:64191701-64191720 | MS.gene66273:intron | 25.0% |
| ATCAGTAAGTATTATTGCAG+TGG | + | chr6.4:64192143-64192162 | MS.gene66273:intron | 30.0% | |
| CATGTTTATGCACTTAACAT+AGG | + | chr6.4:64191470-64191489 | MS.gene66273:CDS | 30.0% | |
| CCTGGAATTATCAAATGTAA+TGG | - | chr6.4:64192481-64192500 | None:intergenic | 30.0% | |
| CTCTTTGCTCTACAAAAAAA+AGG | - | chr6.4:64191606-64191625 | None:intergenic | 30.0% | |
| GGAAAAATTTAGGAACACAA+AGG | + | chr6.4:64192769-64192788 | MS.gene66273:CDS | 30.0% | |
| TACTTTCCATATCTCAACAA+TGG | - | chr6.4:64191741-64191760 | None:intergenic | 30.0% | |
| TAGACTGCATGGAAAAATTT+AGG | + | chr6.4:64192759-64192778 | MS.gene66273:CDS | 30.0% | |
| TGAAGTTATCAAGATTGTCA+TGG | + | chr6.4:64192835-64192854 | MS.gene66273:CDS | 30.0% | |
| TGTCAATATCATGTTGGTAT+CGG | - | chr6.4:64192615-64192634 | None:intergenic | 30.0% | |
| ! | CCATTACATTTGATAATTCC+AGG | + | chr6.4:64192478-64192497 | MS.gene66273:intron | 30.0% |
| ! | TGACTATCCATATAGCTTTT+AGG | - | chr6.4:64192009-64192028 | None:intergenic | 30.0% |
| ! | TTTGCTATACTTTATGTTGG+TGG | + | chr6.4:64192512-64192531 | MS.gene66273:intron | 30.0% |
| !! | TATTTTTGACTGTGACACAT+TGG | - | chr6.4:64191839-64191858 | None:intergenic | 30.0% |
| AATCCTCTTAAGCATAGTCT+TGG | - | chr6.4:64192389-64192408 | None:intergenic | 35.0% | |
| AGCTAGCTAATGATTAATGC+TGG | + | chr6.4:64192688-64192707 | MS.gene66273:intron | 35.0% | |
| CATGTGTTTCCTTTGAATGT+AGG | - | chr6.4:64192797-64192816 | None:intergenic | 35.0% | |
| CTTCAATAACATCATCTGCT+TGG | - | chr6.4:64192820-64192839 | None:intergenic | 35.0% | |
| GTAATTAGATGAAGGGTCAT+TGG | - | chr6.4:64192322-64192341 | None:intergenic | 35.0% | |
| GTTAAGTGCATAAACATGAG+TGG | - | chr6.4:64191468-64191487 | None:intergenic | 35.0% | |
| TAAACTTCATAGCAGGTACA+AGG | - | chr6.4:64192277-64192296 | None:intergenic | 35.0% | |
| TACAAGGCCAATTAGTATAC+TGG | - | chr6.4:64192261-64192280 | None:intergenic | 35.0% | |
| TGTGTGTGTTTGATATCAAG+TGG | - | chr6.4:64192176-64192195 | None:intergenic | 35.0% | |
| TTTCAGACTGAATGTCTGTT+TGG | + | chr6.4:64192569-64192588 | MS.gene66273:intron | 35.0% | |
| TTTCCATATCTCAACAATGG+AGG | - | chr6.4:64191738-64191757 | None:intergenic | 35.0% | |
| ! | TACTGTGGACTATTGTATAG+TGG | + | chr6.4:64191759-64191778 | MS.gene66273:CDS | 35.0% |
| !! | TTGAGATATGGAAAGTACTG+TGG | + | chr6.4:64191744-64191763 | MS.gene66273:CDS | 35.0% |
| ACAAAGGCTCCTACATTCAA+AGG | + | chr6.4:64192785-64192804 | MS.gene66273:CDS | 40.0% | |
| ACTCCAAGACTATGCTTAAG+AGG | + | chr6.4:64192383-64192402 | MS.gene66273:intron | 40.0% | |
| CCAAACATTCATAGACTGCA+TGG | + | chr6.4:64192748-64192767 | MS.gene66273:CDS | 40.0% | |
| CCATGCAGTCTATGAATGTT+TGG | - | chr6.4:64192751-64192770 | None:intergenic | 40.0% | |
| GGGTACATAAACTTCATAGC+AGG | - | chr6.4:64192284-64192303 | None:intergenic | 40.0% | |
| GTTTGGTATCCTACACATGT+TGG | + | chr6.4:64192586-64192605 | MS.gene66273:intron | 40.0% | |
| TGTGAGTCAAGTTTCTGTTC+AGG | + | chr6.4:64191638-64191657 | MS.gene66273:CDS | 40.0% | |
| ! | CATCACAAGGTTTTTCACCA+GGG | - | chr6.4:64191788-64191807 | None:intergenic | 40.0% |
| ! | TAAGCATAGTCTTGGAGTGA+AGG | - | chr6.4:64192381-64192400 | None:intergenic | 40.0% |
| AACTGAAACAGCTACTCCAG+TGG | - | chr6.4:64191504-64191523 | None:intergenic | 45.0% | |
| AGTCTTGGAGTGAAGGAGAA+AGG | - | chr6.4:64192374-64192393 | None:intergenic | 45.0% | |
| CAAGCATCTAGACCATCACA+AGG | - | chr6.4:64191801-64191820 | None:intergenic | 45.0% | |
| CCTGGTGAAAAACCTTGTGA+TGG | + | chr6.4:64191786-64191805 | MS.gene66273:CDS | 45.0% | |
| CTTAGGTAGTCATCTGCAGA+AGG | - | chr6.4:64192716-64192735 | None:intergenic | 45.0% | |
| GTGCCTCCATTGTTGAGATA+TGG | + | chr6.4:64191732-64191751 | MS.gene66273:intron | 45.0% | |
| TATCGGACACCAACATGTGT+AGG | - | chr6.4:64192598-64192617 | None:intergenic | 45.0% | |
| ! | CCATCACAAGGTTTTTCACC+AGG | - | chr6.4:64191789-64191808 | None:intergenic | 45.0% |
| ! | TGTCATGGAAGCTGCTTTAC+TGG | + | chr6.4:64192850-64192869 | MS.gene66273:CDS | 45.0% |
| !! | AACATAGGTGCTGAAACCAC+TGG | + | chr6.4:64191485-64191504 | MS.gene66273:CDS | 45.0% |
| !! | CTATTGTATAGTGGATGCCC+TGG | + | chr6.4:64191768-64191787 | MS.gene66273:CDS | 45.0% |
| ! | ATGGAAGCTGCTTTACTGGC+TGG | + | chr6.4:64192854-64192873 | MS.gene66273:CDS | 50.0% |
| !! | TTTGGCTGCACTGCTGACTT+AGG | - | chr6.4:64192733-64192752 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.4 | gene | 64191407 | 64192898 | 64191407 | ID=MS.gene66273 |
| chr6.4 | mRNA | 64191407 | 64192898 | 64191407 | ID=MS.gene66273.t1;Parent=MS.gene66273 |
| chr6.4 | exon | 64191407 | 64191523 | 64191407 | ID=MS.gene66273.t1.exon1;Parent=MS.gene66273.t1 |
| chr6.4 | CDS | 64191407 | 64191523 | 64191407 | ID=cds.MS.gene66273.t1;Parent=MS.gene66273.t1 |
| chr6.4 | exon | 64191617 | 64191659 | 64191617 | ID=MS.gene66273.t1.exon2;Parent=MS.gene66273.t1 |
| chr6.4 | CDS | 64191617 | 64191659 | 64191617 | ID=cds.MS.gene66273.t1;Parent=MS.gene66273.t1 |
| chr6.4 | exon | 64191733 | 64191858 | 64191733 | ID=MS.gene66273.t1.exon3;Parent=MS.gene66273.t1 |
| chr6.4 | CDS | 64191733 | 64191858 | 64191733 | ID=cds.MS.gene66273.t1;Parent=MS.gene66273.t1 |
| chr6.4 | exon | 64192723 | 64192898 | 64192723 | ID=MS.gene66273.t1.exon4;Parent=MS.gene66273.t1 |
| chr6.4 | CDS | 64192723 | 64192898 | 64192723 | ID=cds.MS.gene66273.t1;Parent=MS.gene66273.t1 |
| Gene Sequence |
| Protein sequence |
