Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66857.t1 | XP_027344974.1 | 100 | 91 | 0 | 0 | 1 | 91 | 185 | 275 | 8.90E-45 | 189.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66857.t1 | Q9SJF0 | 92.3 | 91 | 7 | 0 | 1 | 91 | 204 | 294 | 2.0e-45 | 182.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66857.t1 | G7JET4 | 100.0 | 91 | 0 | 0 | 1 | 91 | 188 | 278 | 6.4e-45 | 189.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050084 | MS.gene66857 | 0.810222 | 1.28E-50 | -1.69E-46 |
| MS.gene050085 | MS.gene66857 | 0.810544 | 1.09E-50 | -1.69E-46 |
| MS.gene050213 | MS.gene66857 | 0.805385 | 1.37E-49 | -1.69E-46 |
| MS.gene050646 | MS.gene66857 | 0.827678 | 1.37E-54 | -1.69E-46 |
| MS.gene051209 | MS.gene66857 | 0.817409 | 3.35E-52 | -1.69E-46 |
| MS.gene052609 | MS.gene66857 | 0.817847 | 2.67E-52 | -1.69E-46 |
| MS.gene052776 | MS.gene66857 | 0.806012 | 1.01E-49 | -1.69E-46 |
| MS.gene053486 | MS.gene66857 | 0.802456 | 5.56E-49 | -1.69E-46 |
| MS.gene056097 | MS.gene66857 | 0.810433 | 1.16E-50 | -1.69E-46 |
| MS.gene057174 | MS.gene66857 | 0.800837 | 1.19E-48 | -1.69E-46 |
| MS.gene059007 | MS.gene66857 | 0.830603 | 2.68E-55 | -1.69E-46 |
| MS.gene060933 | MS.gene66857 | 0.822637 | 2.13E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66857.t1 | MTR_4g050540 | 100.000 | 91 | 0 | 0 | 1 | 91 | 188 | 278 | 2.63e-58 | 192 |
| MS.gene66857.t1 | MTR_2g087530 | 95.604 | 91 | 4 | 0 | 1 | 91 | 71 | 161 | 1.73e-54 | 181 |
| MS.gene66857.t1 | MTR_2g087530 | 95.604 | 91 | 4 | 0 | 1 | 91 | 183 | 273 | 2.56e-54 | 181 |
| MS.gene66857.t1 | MTR_1g089370 | 67.045 | 88 | 29 | 0 | 1 | 88 | 115 | 202 | 3.66e-36 | 129 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66857.t1 | AT2G27210 | 97.802 | 91 | 2 | 0 | 1 | 91 | 193 | 283 | 2.11e-56 | 186 |
| MS.gene66857.t1 | AT2G27210 | 97.802 | 91 | 2 | 0 | 1 | 91 | 193 | 283 | 2.40e-56 | 186 |
| MS.gene66857.t1 | AT1G08420 | 97.802 | 91 | 2 | 0 | 1 | 91 | 204 | 294 | 4.20e-56 | 186 |
| MS.gene66857.t1 | AT1G08420 | 97.802 | 91 | 2 | 0 | 1 | 91 | 204 | 294 | 4.27e-56 | 186 |
| MS.gene66857.t1 | AT4G03080 | 65.909 | 88 | 30 | 0 | 1 | 88 | 115 | 202 | 1.05e-35 | 127 |
| MS.gene66857.t1 | AT1G03445 | 46.591 | 88 | 44 | 2 | 1 | 88 | 115 | 199 | 1.18e-19 | 82.0 |
| MS.gene66857.t1 | AT1G03445 | 46.591 | 88 | 44 | 2 | 1 | 88 | 115 | 199 | 1.26e-19 | 82.0 |
Find 46 sgRNAs with CRISPR-Local
Find 54 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACCTTATGAATGGCGCAAAT+TGG | 0.297374 | 4.4:-51850985 | MS.gene66857:CDS |
| GGAGGCGGGCCCTCTCCTTC+TGG | 0.300169 | 4.4:+51850960 | None:intergenic |
| TTATGTAGAGTTGGTGTTCC+TGG | 0.310667 | 4.4:-51851229 | MS.gene66857:intron |
| GGTGTTCCTGGGCCTGGACC+AGG | 0.320390 | 4.4:-51851217 | MS.gene66857:CDS |
| AGGTTTGGCAGCTGTGTCTA+AGG | 0.367352 | 4.4:+51851004 | None:intergenic |
| TCCAATTTGCGCCATTCATA+AGG | 0.372311 | 4.4:+51850984 | None:intergenic |
| AGAGACCTTTGGCTGATGTA+TGG | 0.418614 | 4.4:-51851028 | MS.gene66857:CDS |
| CAGAGGTATCTCATGGCAAT+TGG | 0.419005 | 4.4:-51851160 | MS.gene66857:CDS |
| CTATGGACATGTTATGGCTT+TGG | 0.433630 | 4.4:-51851188 | MS.gene66857:CDS |
| TTTGCGCCATTCATAAGGTT+TGG | 0.438102 | 4.4:+51850989 | None:intergenic |
| CCATAGCGTGGCCCTGGTCC+AGG | 0.439130 | 4.4:+51851205 | None:intergenic |
| TGGACATGTTATGGCTTTGG+TGG | 0.440355 | 4.4:-51851185 | MS.gene66857:CDS |
| AGAGTTGGTGTTCCTGGGCC+TGG | 0.445637 | 4.4:-51851223 | MS.gene66857:CDS |
| TAATCTAACCTATGCCATCT+TGG | 0.448300 | 4.4:+51851306 | None:intergenic |
| GCCACGCTATGGACATGTTA+TGG | 0.458411 | 4.4:-51851194 | MS.gene66857:CDS |
| GGACATGTTATGGCTTTGGT+GGG | 0.459488 | 4.4:-51851184 | MS.gene66857:CDS |
| CAGGGTGGCATTGGTCCTGC+TGG | 0.463685 | 4.4:-51851375 | MS.gene66857:CDS |
| TTATGGCTTTGGTGGGGCAG+AGG | 0.470740 | 4.4:-51851177 | MS.gene66857:CDS |
| CTCAGTACATGCATGGAGGC+GGG | 0.482872 | 4.4:+51850946 | None:intergenic |
| TATGTAGAGTTGGTGTTCCT+GGG | 0.489689 | 4.4:-51851228 | MS.gene66857:intron |
| CCTGGACCAGGGCCACGCTA+TGG | 0.497047 | 4.4:-51851205 | MS.gene66857:CDS |
| CGTGGCCCTGGTCCAGGCCC+AGG | 0.497591 | 4.4:+51851211 | None:intergenic |
| ACATGTCCATAGCGTGGCCC+TGG | 0.498792 | 4.4:+51851199 | None:intergenic |
| GTGTTCCTGGGCCTGGACCA+GGG | 0.499449 | 4.4:-51851216 | MS.gene66857:CDS |
| ATGGCAATTGGAGGCAATGA+TGG | 0.502919 | 4.4:-51851148 | MS.gene66857:intron |
| ATTGTAAAACAGGGTGGCAT+TGG | 0.507068 | 4.4:-51851384 | None:intergenic |
| CTTAACAGGAAAGAGACCTT+TGG | 0.511121 | 4.4:-51851039 | MS.gene66857:intron |
| GAGACCTTTGGCTGATGTAT+GGG | 0.516196 | 4.4:-51851027 | MS.gene66857:CDS |
| CAGCTGCCAAACCTTATGAA+TGG | 0.521832 | 4.4:-51850995 | MS.gene66857:CDS |
| GCTCAGTACATGCATGGAGG+CGG | 0.528690 | 4.4:+51850945 | None:intergenic |
| AGGTATCTCATGGCAATTGG+AGG | 0.544755 | 4.4:-51851157 | MS.gene66857:CDS |
| GACATGTTATGGCTTTGGTG+GGG | 0.566818 | 4.4:-51851183 | MS.gene66857:CDS |
| CAAATTGGAGCCAGAAGGAG+AGG | 0.568572 | 4.4:-51850970 | MS.gene66857:CDS |
| TCCTCCGCTGACAATCCAGC+AGG | 0.585453 | 4.4:+51851360 | None:intergenic |
| TCATGAGCTCAGTACATGCA+TGG | 0.591658 | 4.4:+51850939 | None:intergenic |
| GGTGGGGCAGAGGTATCTCA+TGG | 0.616684 | 4.4:-51851167 | MS.gene66857:CDS |
| TGGCGCAAATTGGAGCCAGA+AGG | 0.626127 | 4.4:-51850975 | MS.gene66857:CDS |
| TCCTGCTGGATTGTCAGCGG+AGG | 0.627103 | 4.4:-51851361 | MS.gene66857:CDS |
| GCCATAACATGTCCATAGCG+TGG | 0.636565 | 4.4:+51851193 | None:intergenic |
| AAATTGGAGCCAGAAGGAGA+GGG | 0.650187 | 4.4:-51850969 | MS.gene66857:CDS |
| TGGTCCTGCTGGATTGTCAG+CGG | 0.650828 | 4.4:-51851364 | MS.gene66857:CDS |
| TTACACAGCAACGACCAAGA+TGG | 0.657159 | 4.4:-51851320 | MS.gene66857:CDS |
| AAGGCCCATACATCAGCCAA+AGG | 0.663227 | 4.4:+51851023 | None:intergenic |
| GTGTAAGATCAAGAACGTGA+AGG | 0.669769 | 4.4:+51851337 | None:intergenic |
| AGCAACGACCAAGATGGCAT+AGG | 0.705668 | 4.4:-51851314 | MS.gene66857:intron |
| TGAGCTCAGTACATGCATGG+AGG | 0.728339 | 4.4:+51850942 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TCAAATATGTAAAAGCATTT+GGG | + | chr4.4:51851210-51851229 | None:intergenic | 20.0% |
| ! | ATTACAAATGAAGTAGAGAT+AGG | + | chr4.4:51851050-51851069 | None:intergenic | 25.0% |
| ! | TTACAAATGAAGTAGAGATA+GGG | + | chr4.4:51851049-51851068 | None:intergenic | 25.0% |
| !! | ATGCTTTTACATATTTGACT+TGG | - | chr4.4:51851212-51851231 | MS.gene66857:CDS | 25.0% |
| !!! | GTCAAATATGTAAAAGCATT+TGG | + | chr4.4:51851211-51851230 | None:intergenic | 25.0% |
| !! | AATATGTAAAAGCATTTGGG+TGG | + | chr4.4:51851207-51851226 | None:intergenic | 30.0% |
| TAATCTAACCTATGCCATCT+TGG | + | chr4.4:51851023-51851042 | None:intergenic | 35.0% | |
| TTCTGGATGTTATGTAGAGT+TGG | - | chr4.4:51851088-51851107 | MS.gene66857:intron | 35.0% | |
| AAGGTCTCTTTCCTGTTAAG+TGG | + | chr4.4:51851287-51851306 | None:intergenic | 40.0% | |
| ACCTTATGAATGGCGCAAAT+TGG | - | chr4.4:51851341-51851360 | MS.gene66857:CDS | 40.0% | |
| AGGTCTCTTTCCTGTTAAGT+GGG | + | chr4.4:51851286-51851305 | None:intergenic | 40.0% | |
| CTATGGACATGTTATGGCTT+TGG | - | chr4.4:51851138-51851157 | MS.gene66857:intron | 40.0% | |
| CTTAACAGGAAAGAGACCTT+TGG | - | chr4.4:51851287-51851306 | MS.gene66857:intron | 40.0% | |
| GTGTAAGATCAAGAACGTGA+AGG | + | chr4.4:51850992-51851011 | None:intergenic | 40.0% | |
| TCCAATTTGCGCCATTCATA+AGG | + | chr4.4:51851345-51851364 | None:intergenic | 40.0% | |
| TTTGCGCCATTCATAAGGTT+TGG | + | chr4.4:51851340-51851359 | None:intergenic | 40.0% | |
| !! | TATGTAGAGTTGGTGTTCCT+GGG | - | chr4.4:51851098-51851117 | MS.gene66857:intron | 40.0% |
| !! | TTATGTAGAGTTGGTGTTCC+TGG | - | chr4.4:51851097-51851116 | MS.gene66857:intron | 40.0% |
| !! | TTGTTTTACCCCCACTTAAC+AGG | - | chr4.4:51851273-51851292 | MS.gene66857:intron | 40.0% |
| AAATTGGAGCCAGAAGGAGA+GGG | - | chr4.4:51851357-51851376 | MS.gene66857:CDS | 45.0% | |
| AGAGACCTTTGGCTGATGTA+TGG | - | chr4.4:51851298-51851317 | MS.gene66857:intron | 45.0% | |
| AGGTATCTCATGGCAATTGG+AGG | - | chr4.4:51851169-51851188 | MS.gene66857:CDS | 45.0% | |
| ATGGCAATTGGAGGCAATGA+TGG | - | chr4.4:51851178-51851197 | MS.gene66857:CDS | 45.0% | |
| CAGCTGCCAAACCTTATGAA+TGG | - | chr4.4:51851331-51851350 | MS.gene66857:CDS | 45.0% | |
| GAGACCTTTGGCTGATGTAT+GGG | - | chr4.4:51851299-51851318 | MS.gene66857:intron | 45.0% | |
| GCATTCATCTGACTCTGTTC+TGG | - | chr4.4:51851071-51851090 | MS.gene66857:intron | 45.0% | |
| GGTCTCTTTCCTGTTAAGTG+GGG | + | chr4.4:51851285-51851304 | None:intergenic | 45.0% | |
| GTCTCTTTCCTGTTAAGTGG+GGG | + | chr4.4:51851284-51851303 | None:intergenic | 45.0% | |
| TTACACAGCAACGACCAAGA+TGG | - | chr4.4:51851006-51851025 | MS.gene66857:CDS | 45.0% | |
| !! | CAGAGGTATCTCATGGCAAT+TGG | - | chr4.4:51851166-51851185 | MS.gene66857:CDS | 45.0% |
| !! | GACATGTTATGGCTTTGGTG+GGG | - | chr4.4:51851143-51851162 | MS.gene66857:intron | 45.0% |
| !! | GGACATGTTATGGCTTTGGT+GGG | - | chr4.4:51851142-51851161 | MS.gene66857:intron | 45.0% |
| !! | TGGACATGTTATGGCTTTGG+TGG | - | chr4.4:51851141-51851160 | MS.gene66857:intron | 45.0% |
| AAGGCCCATACATCAGCCAA+AGG | + | chr4.4:51851306-51851325 | None:intergenic | 50.0% | |
| AGCAACGACCAAGATGGCAT+AGG | - | chr4.4:51851012-51851031 | MS.gene66857:CDS | 50.0% | |
| AGGTTTGGCAGCTGTGTCTA+AGG | + | chr4.4:51851325-51851344 | None:intergenic | 50.0% | |
| CAAATTGGAGCCAGAAGGAG+AGG | - | chr4.4:51851356-51851375 | MS.gene66857:CDS | 50.0% | |
| GCCACGCTATGGACATGTTA+TGG | - | chr4.4:51851132-51851151 | MS.gene66857:intron | 50.0% | |
| GCCATAACATGTCCATAGCG+TGG | + | chr4.4:51851136-51851155 | None:intergenic | 50.0% | |
| TGGCGCAAATTGGAGCCAGA+AGG | - | chr4.4:51851351-51851370 | MS.gene66857:CDS | 55.0% | |
| TGGTCCTGCTGGATTGTCAG+CGG | - | chr4.4:51850962-51850981 | MS.gene66857:CDS | 55.0% | |
| !! | TTATGGCTTTGGTGGGGCAG+AGG | - | chr4.4:51851149-51851168 | MS.gene66857:CDS | 55.0% |
| ACATGTCCATAGCGTGGCCC+TGG | + | chr4.4:51851130-51851149 | None:intergenic | 60.0% | |
| TCCTCCGCTGACAATCCAGC+AGG | + | chr4.4:51850969-51850988 | None:intergenic | 60.0% | |
| TCCTGCTGGATTGTCAGCGG+AGG | - | chr4.4:51850965-51850984 | MS.gene66857:CDS | 60.0% | |
| !! | AGAGTTGGTGTTCCTGGGCC+TGG | - | chr4.4:51851103-51851122 | MS.gene66857:intron | 60.0% |
| !! | GGTGGGGCAGAGGTATCTCA+TGG | - | chr4.4:51851159-51851178 | MS.gene66857:CDS | 60.0% |
| GTGTTCCTGGGCCTGGACCA+GGG | - | chr4.4:51851110-51851129 | MS.gene66857:intron | 65.0% | |
| AGAGGGCCCGCCTCCATGCA+TGG | - | chr4.4:51851374-51851393 | MS.gene66857:CDS | 70.0% | |
| CCATAGCGTGGCCCTGGTCC+AGG | + | chr4.4:51851124-51851143 | None:intergenic | 70.0% | |
| CCTGGACCAGGGCCACGCTA+TGG | - | chr4.4:51851121-51851140 | MS.gene66857:intron | 70.0% | |
| GGTGTTCCTGGGCCTGGACC+AGG | - | chr4.4:51851109-51851128 | MS.gene66857:intron | 70.0% | |
| GGAGGCGGGCCCTCTCCTTC+TGG | + | chr4.4:51851369-51851388 | None:intergenic | 75.0% | |
| ! | CGTGGCCCTGGTCCAGGCCC+AGG | + | chr4.4:51851118-51851137 | None:intergenic | 80.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 51850954 | 51851394 | 51850954 | ID=MS.gene66857 |
| chr4.4 | mRNA | 51850954 | 51851394 | 51850954 | ID=MS.gene66857.t1;Parent=MS.gene66857 |
| chr4.4 | exon | 51851315 | 51851394 | 51851315 | ID=MS.gene66857.t1.exon1;Parent=MS.gene66857.t1 |
| chr4.4 | CDS | 51851315 | 51851394 | 51851315 | ID=cds.MS.gene66857.t1;Parent=MS.gene66857.t1 |
| chr4.4 | exon | 51851149 | 51851243 | 51851149 | ID=MS.gene66857.t1.exon2;Parent=MS.gene66857.t1 |
| chr4.4 | CDS | 51851149 | 51851243 | 51851149 | ID=cds.MS.gene66857.t1;Parent=MS.gene66857.t1 |
| chr4.4 | exon | 51850954 | 51851053 | 51850954 | ID=MS.gene66857.t1.exon3;Parent=MS.gene66857.t1 |
| chr4.4 | CDS | 51850954 | 51851053 | 51850954 | ID=cds.MS.gene66857.t1;Parent=MS.gene66857.t1 |
| Gene Sequence |
| Protein sequence |
