AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene67063

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene67063.t1 MTR_3g107280 97.348 641 17 0 1 641 1 641 0.0 1250
MS.gene67063.t1 MTR_1g012520 66.667 645 207 4 1 640 1 642 0.0 847
MS.gene67063.t1 MTR_4g114640 55.167 600 265 3 44 640 34 632 0.0 601
MS.gene67063.t1 MTR_4g114650 50.912 603 255 5 44 641 33 599 0.0 551
MS.gene67063.t1 MTR_4g114650 50.912 603 255 5 44 641 37 603 0.0 551
MS.gene67063.t1 MTR_4g114650 50.912 603 255 5 44 641 88 654 0.0 551
MS.gene67063.t1 MTR_2g021030 25.316 553 356 15 71 579 11 550 1.66e-35 142
MS.gene67063.t1 MTR_2g021030 27.574 272 172 3 172 419 305 575 2.79e-23 105
MS.gene67063.t1 MTR_2g021030 26.296 270 178 5 143 406 334 588 1.13e-18 90.5
MS.gene67063.t1 MTR_4g013255 27.565 497 302 17 137 605 133 599 1.10e-28 122
MS.gene67063.t1 MTR_4g013255 25.460 326 208 8 174 499 320 610 1.46e-20 96.7
MS.gene67063.t1 MTR_4g013255 26.199 271 164 6 135 404 336 571 9.52e-16 81.3
MS.gene67063.t1 MTR_1g114610 27.411 394 256 12 69 445 18 398 3.15e-27 115
MS.gene67063.t1 MTR_1g114610 23.913 322 213 5 304 623 95 386 1.46e-16 82.8
MS.gene67063.t1 MTR_1g114610 28.352 261 153 7 140 372 145 399 3.20e-15 78.6
MS.gene67063.t1 MTR_1g114610 28.409 176 123 3 456 630 113 286 7.38e-13 71.2
MS.gene67063.t1 MTR_2g021030 27.488 422 261 15 71 466 11 413 4.94e-25 108
MS.gene67063.t1 MTR_2g021030 25.786 318 204 9 330 622 127 437 1.79e-20 95.1
MS.gene67063.t1 MTR_2g021030 25.523 239 148 5 174 412 231 439 3.34e-16 82.0
MS.gene67063.t1 MTR_2g021030 36.522 115 73 0 172 286 305 419 1.26e-15 80.1
MS.gene67063.t1 MTR_7g112570 25.909 440 288 13 169 595 190 604 8.58e-24 106
MS.gene67063.t1 MTR_7g112570 25.316 474 263 17 205 623 94 531 6.64e-20 94.4
MS.gene67063.t1 MTR_7g112570 26.450 431 277 12 169 571 190 608 8.80e-24 106
MS.gene67063.t1 MTR_7g112570 23.473 311 207 5 174 459 350 654 3.37e-16 82.8
MS.gene67063.t1 MTR_7g112570 24.403 377 233 13 266 606 103 463 3.51e-14 76.3
MS.gene67063.t1 MTR_7g112570 26.303 422 276 12 169 577 190 589 1.74e-23 105
MS.gene67063.t1 MTR_7g112570 25.316 474 263 17 205 623 94 531 6.74e-20 94.4
MS.gene67063.t1 MTR_1g054625 30.769 247 154 9 343 585 107 340 2.61e-20 93.2
MS.gene67063.t1 MTR_1g054625 27.119 236 159 5 142 372 89 316 9.98e-14 73.6
MS.gene67063.t1 MTR_1g054625 26.016 246 163 6 172 407 90 326 1.25e-12 70.1
MS.gene67063.t1 MTR_1g054625 22.924 301 173 7 213 484 79 349 8.42e-11 64.3
MS.gene67063.t1 MTR_8g028140 26.984 378 238 10 66 423 17 376 5.25e-20 93.2
MS.gene67063.t1 MTR_8g028140 21.858 366 216 7 191 549 66 368 2.14e-11 66.6
MS.gene67063.t1 MTR_1g054625 30.453 243 152 9 347 585 14 243 5.56e-19 87.4
MS.gene67063.t1 MTR_1g054625 23.904 251 158 5 160 407 9 229 2.73e-14 73.6
MS.gene67063.t1 MTR_1g054625 25.668 187 131 3 330 515 51 230 3.52e-11 64.3
MS.gene67063.t1 MTR_8g028140 28.058 278 174 6 159 423 32 296 5.92e-18 85.9
MS.gene67063.t1 MTR_8g028140 24.671 304 178 8 266 549 16 288 6.76e-11 64.3
MS.gene67063.t1 MTR_3g088895 25.926 216 107 3 196 411 111 273 2.96e-16 81.3
MS.gene67063.t1 MTR_1g015450 24.494 445 226 15 174 580 327 699 2.99e-16 83.2
MS.gene67063.t1 MTR_4g019320 20.755 424 292 8 178 577 122 525 5.15e-16 82.0
MS.gene67063.t1 MTR_1g029500 23.404 282 147 4 196 461 98 326 1.05e-15 79.3
MS.gene67063.t1 MTR_1g029500 31.507 146 73 3 141 262 127 269 1.01e-11 67.4
MS.gene67063.t1 MTR_4g009030 25.236 424 272 16 173 571 127 530 2.63e-15 79.7
MS.gene67063.t1 MTR_4g009030 22.432 584 361 20 68 632 3 513 4.38e-13 72.8
MS.gene67063.t1 MTR_1g015480 23.557 433 221 14 186 580 157 517 1.58e-14 77.8
MS.gene67063.t1 MTR_1g015480 23.256 473 274 16 173 577 164 615 1.31e-11 68.2
MS.gene67063.t1 MTR_4g130620 30.726 179 108 5 227 403 102 266 1.49e-13 72.4
MS.gene67063.t1 MTR_5g095830 22.440 459 268 13 178 593 149 562 7.27e-13 72.0
MS.gene67063.t1 MTR_4g133640 21.769 441 289 11 178 576 135 561 2.23e-12 70.5
MS.gene67063.t1 MTR_4g133640 23.892 406 239 15 160 517 172 555 4.76e-11 66.2
MS.gene67063.t1 MTR_1g018790 23.978 367 199 12 225 580 137 434 1.46e-11 67.8
MS.gene67063.t1 MTR_3g006050 24.332 374 214 11 200 514 192 555 6.16e-11 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene67063.t1 AT2G25490 56.875 640 263 6 1 640 1 627 0.0 679
MS.gene67063.t1 AT5G25350 52.100 643 286 6 1 641 1 623 0.0 611
MS.gene67063.t1 AT4G15475 28.007 582 378 19 71 641 11 562 4.45e-48 178
MS.gene67063.t1 AT4G15475 27.762 353 247 6 174 526 231 575 2.23e-31 130
MS.gene67063.t1 AT4G15475 26.531 343 241 6 123 460 252 588 1.28e-29 124
MS.gene67063.t1 AT5G27920 29.083 447 275 16 173 604 158 577 1.88e-28 121
MS.gene67063.t1 AT5G27920 26.351 296 185 7 200 495 338 600 5.13e-14 75.9
MS.gene67063.t1 AT5G27920 25.381 394 251 13 265 627 65 446 4.20e-12 69.7
MS.gene67063.t1 AT5G23340 28.881 277 189 6 304 577 85 356 4.07e-25 108
MS.gene67063.t1 AT5G23340 25.201 373 261 11 69 433 8 370 4.94e-23 102
MS.gene67063.t1 AT5G23340 25.442 283 181 3 349 630 76 329 1.28e-16 82.8
MS.gene67063.t1 AT5G23340 29.213 178 123 3 456 632 103 278 3.22e-14 75.5
MS.gene67063.t1 AT5G01720 23.232 396 249 6 176 548 241 604 2.43e-23 105
MS.gene67063.t1 AT5G01720 26.096 479 310 15 174 623 85 548 4.95e-19 92.0
MS.gene67063.t1 AT5G01720 22.593 270 175 6 173 415 315 577 8.59e-14 75.1
MS.gene67063.t1 AT1G77000 25.000 216 109 2 196 411 100 262 9.23e-16 79.7
MS.gene67063.t1 AT1G77000 25.000 216 109 2 196 411 100 262 9.23e-16 79.7
MS.gene67063.t1 AT1G21410 24.537 216 110 2 196 411 100 262 9.31e-16 79.7
MS.gene67063.t1 AT1G21410 24.537 216 110 2 196 411 100 262 9.31e-16 79.7
MS.gene67063.t1 AT1G55590 23.767 223 165 3 172 392 296 515 2.38e-14 77.0
MS.gene67063.t1 AT3G58530 26.866 268 159 7 343 607 109 342 3.42e-14 75.1
MS.gene67063.t1 AT3G58530 23.140 242 148 4 152 393 102 305 2.66e-12 69.3
MS.gene67063.t1 AT4G33210 24.015 533 301 20 128 616 321 793 5.57e-14 76.3
MS.gene67063.t1 AT1G47056 28.028 289 148 9 345 580 130 411 3.58e-13 72.8
MS.gene67063.t1 AT1G47056 33.523 176 100 8 117 286 244 408 7.72e-13 71.6
MS.gene67063.t1 AT1G47056 23.210 461 292 17 43 490 15 426 2.67e-11 67.0
MS.gene67063.t1 AT3G07550 25.193 389 242 9 61 423 8 373 4.32e-13 72.0
MS.gene67063.t1 AT3G07550 25.193 389 242 9 61 423 8 373 4.32e-13 72.0
MS.gene67063.t1 AT4G07400 38.211 123 73 3 160 282 344 463 8.59e-12 68.6
MS.gene67063.t1 AT1G80630 23.000 400 239 12 198 567 148 508 2.98e-11 67.0
MS.gene67063.t1 AT3G07550 24.936 389 216 11 61 423 8 346 3.02e-11 65.9

Find 158 sgRNAs with CRISPR-Local

Find 198 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCTGAAGTCTTTCACTCTT+TGG 0.100387 3.4:-93225494 MS.gene67063:CDS
GATTACTCACTAGCAGTTAT+TGG 0.193905 3.4:-93225141 MS.gene67063:CDS
TGCCATAGGATTACCCAATT+TGG 0.212481 3.4:-93224838 MS.gene67063:CDS
TGACACCGGCAACCCCAAAT+TGG 0.224942 3.4:+93224824 None:intergenic
TCTGAAGTCTTTCACTCTTT+GGG 0.233451 3.4:-93225493 MS.gene67063:CDS
CAATCTGGATGTACTTTCTT+TGG 0.236470 3.4:-93224308 MS.gene67063:CDS
CTTGGAGTGCTAAACCTTAA+TGG 0.242738 3.4:-93224460 MS.gene67063:CDS
TCGAAGCTTGGAGGGGAAAA+AGG 0.250888 3.4:-93225655 MS.gene67063:CDS
GTTGCTGCAGATTTCATTCT+TGG 0.268042 3.4:+93225737 None:intergenic
GCCATAGGATTACCCAATTT+GGG 0.271797 3.4:-93224837 MS.gene67063:CDS
GTCCTTCAGGCAACCTTCTA+AGG 0.271825 3.4:+93225820 None:intergenic
TGCCCTGGAGTAACAGATAT+TGG 0.295544 3.4:-93224994 MS.gene67063:CDS
ACACAATCTCGAGGAGGATT+AGG 0.296188 3.4:-93225597 MS.gene67063:CDS
AAACCTTAATGGTTGTAAAA+AGG 0.296888 3.4:-93224449 MS.gene67063:CDS
ATCTGCAGCAACGCAAGTTC+TGG 0.313753 3.4:-93225726 MS.gene67063:CDS
GACGGTGACAATCAAGAATT+TGG 0.313887 3.4:-93225696 MS.gene67063:CDS
ATTGATACGGACCTTAGATT+TGG 0.325991 3.4:+93225264 None:intergenic
TTCACTGCTTCAGAGGTCTA+AGG 0.330025 3.4:-93224569 MS.gene67063:CDS
CAACTAACGTCGCTCACAAT+TGG 0.335014 3.4:-93225021 MS.gene67063:CDS
TAATGGATGCTGAACATAAT+AGG 0.341815 3.4:+93225202 None:intergenic
AATTGTAGGCTCACAATTGA+TGG 0.346716 3.4:+93224868 None:intergenic
CTTCAGTGTCTTGAATTGAT+TGG 0.348304 3.4:-93224622 MS.gene67063:CDS
TCAGAGGTCTAAGGCCAGTT+TGG 0.348388 3.4:-93224560 MS.gene67063:CDS
TTCTTTGGCAGGTTGCTCAT+TGG 0.352209 3.4:-93224293 MS.gene67063:CDS
ATTACCCAATTTGGGGTTGC+CGG 0.354313 3.4:-93224829 MS.gene67063:CDS
ATGTCACCGAGAAAGGATTC+TGG 0.362806 3.4:-93225071 MS.gene67063:CDS
TAGATTAACATTACCCAAAC+TGG 0.367200 3.4:+93224546 None:intergenic
TTAGGGAAGCTTTCAATTCA+TGG 0.367968 3.4:-93225579 MS.gene67063:CDS
AAGCTCCTATCTTGTCCTTC+AGG 0.374082 3.4:+93225807 None:intergenic
AATCCTTTCTCGGTGACATT+TGG 0.374117 3.4:+93225075 None:intergenic
CTAGCAGTTATTGGACAATA+TGG 0.374694 3.4:-93225132 MS.gene67063:CDS
AGAAGAGACCCGCCTCCTTC+GGG 0.379204 3.4:+93225991 None:intergenic
GAAAGTTGTGTCCAAATCTA+AGG 0.379486 3.4:-93225275 MS.gene67063:CDS
CTCACAATTGGGCTGTGCCC+TGG 0.383441 3.4:-93225009 MS.gene67063:CDS
AAGTTGCATTGTTCGACTCT+TGG 0.384757 3.4:-93224478 MS.gene67063:CDS
GACCCGCCTCCTTCGGGTTA+AGG 0.385671 3.4:+93225997 None:intergenic
GCGGGTCTCTTCTTGTCTCT+TGG 0.388125 3.4:-93225981 MS.gene67063:CDS
TCTAAAGTTCTCAGCTTTAC+CGG 0.389616 3.4:-93226665 MS.gene67063:intron
CAGAGGTCTAAGGCCAGTTT+GGG 0.390240 3.4:-93224559 MS.gene67063:CDS
AATGAAGGTCTACATGCTAT+TGG 0.392573 3.4:-93225297 MS.gene67063:CDS
CTTCCAAATGTCACCGAGAA+AGG 0.393704 3.4:-93225078 MS.gene67063:CDS
GCCCTGGAGTAACAGATATT+GGG 0.394824 3.4:-93224993 MS.gene67063:CDS
GCCTCTTTGAGATCCTTAGA+AGG 0.399791 3.4:-93225833 MS.gene67063:CDS
CTGTCCTGGAGTTCGTGATC+AGG 0.403623 3.4:-93225235 MS.gene67063:CDS
CACAATCTCGAGGAGGATTA+GGG 0.405866 3.4:-93225596 MS.gene67063:CDS
CTGGATGTACTTTCTTTGGC+AGG 0.406723 3.4:-93224304 MS.gene67063:CDS
AAGAAGAGACCCGCCTCCTT+CGG 0.411237 3.4:+93225990 None:intergenic
CTTGAATTGATTGGACTTGA+GGG 0.412726 3.4:-93224613 MS.gene67063:CDS
ATCAATTGTGAGCCTACAAT+TGG 0.415296 3.4:-93224866 MS.gene67063:CDS
TACCAACTCCAGGGCAGTTA+CGG 0.416240 3.4:+93224683 None:intergenic
AAAATTGAAGGCTCTTACTC+TGG 0.417528 3.4:-93224785 MS.gene67063:CDS
TATGGCACTCTTCCAATTGT+AGG 0.418212 3.4:+93224854 None:intergenic
ACAAGCTTGTGGAACGTCTC+TGG 0.423025 3.4:-93224174 MS.gene67063:CDS
CACAATTGATGGAGCAGCCT+TGG 0.432002 3.4:+93224879 None:intergenic
AACTAACGTCGCTCACAATT+GGG 0.438220 3.4:-93225020 MS.gene67063:CDS
CTTACTCTGGTAAGCTGCTA+CGG 0.439956 3.4:-93224772 MS.gene67063:CDS
GTTCGTGATCAGGGAATTGC+TGG 0.441466 3.4:-93225225 MS.gene67063:CDS
CATCACCCAGAATCCTTTCT+CGG 0.442853 3.4:+93225065 None:intergenic
GGCTTGATCGAGATTGCTAA+TGG 0.449362 3.4:-93225447 MS.gene67063:CDS
TGCATCGGTGATTGCACACT+CGG 0.454668 3.4:+93224363 None:intergenic
ATCGATAACTCAGTCAGATT+CGG 0.457316 3.4:+93225342 None:intergenic
ACATCAGTCAAGGCACGATC+TGG 0.458394 3.4:+93225549 None:intergenic
AAACCGTGGTGCAAAATTGA+AGG 0.459034 3.4:-93224797 MS.gene67063:CDS
AATCAAGAATTTGGTGATGA+AGG 0.460069 3.4:-93225687 MS.gene67063:CDS
TGAACAGGACTCCACGAGTA+AGG 0.461905 3.4:+93224329 None:intergenic
GAGTGTGCAATCACCGATGC+AGG 0.462800 3.4:-93224361 MS.gene67063:CDS
GACACCGGCAACCCCAAATT+GGG 0.463002 3.4:+93224825 None:intergenic
ATCTCAAAGAGGCATTCATC+AGG 0.464081 3.4:+93225843 None:intergenic
GATCAAGCCTTTGTCACTAA+TGG 0.465229 3.4:+93225461 None:intergenic
TCGTGGAGTCCTGTTCAATC+TGG 0.467985 3.4:-93224323 MS.gene67063:CDS
TATTGGACAATATGGCTTTG+TGG 0.469637 3.4:-93225124 MS.gene67063:CDS
GGTTGCCTGAAGGACAAGAT+AGG 0.472680 3.4:-93225812 MS.gene67063:CDS
AGCCCAATATCTGTTACTCC+AGG 0.474848 3.4:+93224991 None:intergenic
GGATTCTGGGTGATGGGTAA+TGG 0.476172 3.4:-93225057 MS.gene67063:CDS
AATGTCCCATTTGACTTCAG+TGG 0.477222 3.4:-93225912 MS.gene67063:CDS
TTTCTCCACTGAAGTCAAAT+GGG 0.478494 3.4:+93225907 None:intergenic
TAGAAGCGTGAGCCAGCGCT+TGG 0.480253 3.4:+93225770 None:intergenic
AGGCTATCTTTCTCGAAGCT+TGG 0.481683 3.4:-93225667 MS.gene67063:CDS
TTATCAATCCGTAACTGCCC+TGG 0.487556 3.4:-93224691 MS.gene67063:CDS
TTGGGTAATGTTAATCTAAG+TGG 0.488310 3.4:-93224541 MS.gene67063:CDS
AAAGTACATCCAGATTGAAC+AGG 0.488581 3.4:+93224314 None:intergenic
GTCTGTAAGCATATTCTTGA+CGG 0.492252 3.4:+93225958 None:intergenic
CTATCTTTCTCGAAGCTTGG+AGG 0.492264 3.4:-93225664 MS.gene67063:CDS
GGGCTTGAAGCTGTAGGAAA+GGG 0.497092 3.4:-93224973 MS.gene67063:CDS
GGTCTCAAGGCAGTTGCTCA+TGG 0.502544 3.4:-93225525 MS.gene67063:CDS
CCCCAAATTGGGTAATCCTA+TGG 0.504344 3.4:+93224836 None:intergenic
TACCTTAACCCGAAGGAGGC+GGG 0.504347 3.4:-93225999 MS.gene67063:CDS
TGTCACCGAGAAAGGATTCT+GGG 0.505645 3.4:-93225070 MS.gene67063:CDS
ACGTTAGTTGCTGCAGTGCA+TGG 0.507163 3.4:+93225034 None:intergenic
AAAGCCTTGTCAGAAATTGT+TGG 0.508372 3.4:+93225387 None:intergenic
ATCCGTAACTGCCCTGGAGT+TGG 0.514000 3.4:-93224685 MS.gene67063:CDS
CGAGAAAGGATTCTGGGTGA+TGG 0.515420 3.4:-93225064 MS.gene67063:CDS
GTGCTTCTGTATCCAAGCGC+TGG 0.517996 3.4:-93225782 MS.gene67063:CDS
CTTTCTCGGTGACATTTGGA+AGG 0.518084 3.4:+93225079 None:intergenic
AGAGTAAAATTACCAACTCC+AGG 0.518751 3.4:+93224673 None:intergenic
GGGATTTCTGCCCTTACTCG+TGG 0.522818 3.4:-93224340 MS.gene67063:CDS
GGTTTATTTCACTGCTTCAG+AGG 0.535734 3.4:-93224576 MS.gene67063:CDS
TGAAGCAGTGAAATAAACCC+TGG 0.539869 3.4:+93224580 None:intergenic
AGACTAGCTGCCATTGCTGT+TGG 0.540189 3.4:-93225621 MS.gene67063:CDS
TGTCCTAGCATCGGTAATGA+AGG 0.540249 3.4:-93225312 MS.gene67063:CDS
TATCTTTCTCGAAGCTTGGA+GGG 0.544169 3.4:-93225663 MS.gene67063:CDS
AAATTGTTGGCAACTTGCAT+AGG 0.545096 3.4:+93225400 None:intergenic
GTCTGACATGGAGATACTGC+TGG 0.549571 3.4:+93224724 None:intergenic
CAACGCAAGTTCTGGAAACA+AGG 0.552191 3.4:-93225718 MS.gene67063:CDS
TCTTGAATTGATTGGACTTG+AGG 0.552643 3.4:-93224614 MS.gene67063:CDS
TTACTCTGGTAAGCTGCTAC+GGG 0.552800 3.4:-93224771 MS.gene67063:CDS
TGTCCTGGAGTTCGTGATCA+GGG 0.555690 3.4:-93225234 MS.gene67063:CDS
ATCTTTCTCGAAGCTTGGAG+GGG 0.555765 3.4:-93225662 MS.gene67063:CDS
GTACCTTAACCCGAAGGAGG+CGG 0.556569 3.4:-93226000 MS.gene67063:CDS
GTGCATTTCTGTCAGACAAT+GGG 0.557102 3.4:-93224915 MS.gene67063:CDS
TAATGGTTGTCATCAGATAG+AGG 0.558248 3.4:-93225430 MS.gene67063:CDS
TGGGCTTGAAGCTGTAGGAA+AGG 0.558947 3.4:-93224974 MS.gene67063:CDS
CTTGACTGATGTAGGTCTCA+AGG 0.565875 3.4:-93225538 MS.gene67063:CDS
AATTCAAGACACTGAAGCAT+AGG 0.567525 3.4:+93224628 None:intergenic
GAGTGAAAGACTTCAGAGAA+GGG 0.568710 3.4:+93225499 None:intergenic
GAGAAAGGATTCTGGGTGAT+GGG 0.569849 3.4:-93225063 MS.gene67063:CDS
TGCATCAATCTGACAGATGT+AGG 0.573775 3.4:-93224517 MS.gene67063:CDS
ATTCCCTGATCACGAACTCC+AGG 0.574036 3.4:+93225231 None:intergenic
GATATTGGGCTTGAAGCTGT+AGG 0.576495 3.4:-93224979 MS.gene67063:CDS
AGAGTGAAAGACTTCAGAGA+AGG 0.580309 3.4:+93225498 None:intergenic
TGTGCATTTCTGTCAGACAA+TGG 0.584416 3.4:-93224916 MS.gene67063:CDS
AAATTGGGTGATTCCCTCGA+GGG 0.585090 3.4:-93224241 MS.gene67063:CDS
ATTGTCTGCAATTGCTGTCA+AGG 0.587916 3.4:+93224414 None:intergenic
AGATTGTGTGCCAACAGCAA+TGG 0.591886 3.4:+93225611 None:intergenic
GAACAGGACTCCACGAGTAA+GGG 0.593363 3.4:+93224330 None:intergenic
TTTCTCGGTGACATTTGGAA+GGG 0.597962 3.4:+93225080 None:intergenic
GTTGCTACCATTAGTGACAA+AGG 0.599127 3.4:-93225468 MS.gene67063:CDS
CAAGGCTACCTGCTCAGAAG+AGG 0.600220 3.4:+93224140 None:intergenic
CCGCACAGTTAACAAGCTTG+TGG 0.604170 3.4:-93224185 MS.gene67063:CDS
GATCGTGCCTTGACTGATGT+AGG 0.605270 3.4:-93225546 MS.gene67063:CDS
CCATAGGATTACCCAATTTG+GGG 0.605745 3.4:-93224836 MS.gene67063:CDS
GTTGCCAACAATTTCTGACA+AGG 0.608947 3.4:-93225391 MS.gene67063:CDS
AAGGGCAGAAATCCCTGCAT+CGG 0.611779 3.4:+93224348 None:intergenic
ATTTCTGTCAGACAATGGGC+TGG 0.612009 3.4:-93224911 MS.gene67063:CDS
TACAATTGGAAGAGTGCCAT+AGG 0.616497 3.4:-93224852 MS.gene67063:CDS
CTTGAGGGAATAACAGACCC+AGG 0.616547 3.4:-93224598 MS.gene67063:CDS
ATCCTTAGAAGGTTGCCTGA+AGG 0.616856 3.4:-93225822 MS.gene67063:CDS
ATAGAGTCATGTCCTAGCAT+CGG 0.621372 3.4:-93225321 MS.gene67063:CDS
CGAACAATGCAACTTGACCA+TGG 0.623330 3.4:+93224486 None:intergenic
GAAGCAGTGAAATAAACCCT+GGG 0.624828 3.4:+93224581 None:intergenic
ACCACGGTTTAAAATGACAC+CGG 0.630252 3.4:+93224810 None:intergenic
ACCTTCTAAGGATCTCAAAG+AGG 0.631936 3.4:+93225832 None:intergenic
AGTGTGCAATCACCGATGCA+GGG 0.633058 3.4:-93224360 MS.gene67063:CDS
GCCCAATATCTGTTACTCCA+GGG 0.638985 3.4:+93224992 None:intergenic
AATGACGAAATTGTCTGACA+TGG 0.645410 3.4:+93224712 None:intergenic
GTCGATGTACCTTAACCCGA+AGG 0.647280 3.4:-93226006 MS.gene67063:CDS
CTTGAGACCTACATCAGTCA+AGG 0.649066 3.4:+93225539 None:intergenic
AGACCTTCATTACCGATGCT+AGG 0.654284 3.4:+93225309 None:intergenic
CCACAAGCTTGTTAACTGTG+CGG 0.656307 3.4:+93224185 None:intergenic
GAGTAAAATTACCAACTCCA+GGG 0.662405 3.4:+93224674 None:intergenic
GCAAGTTCTGGAAACAAGGA+CGG 0.662723 3.4:-93225714 MS.gene67063:CDS
GCATAGGACAGAGCTTCCCA+AGG 0.666207 3.4:+93224644 None:intergenic
TTGAGGGAATAACAGACCCA+GGG 0.669478 3.4:-93224597 MS.gene67063:CDS
GATGTACCTTAACCCGAAGG+AGG 0.670839 3.4:-93226003 MS.gene67063:CDS
GCTGTTGGCACACAATCTCG+AGG 0.687326 3.4:-93225606 MS.gene67063:CDS
AAAATTGGGTGATTCCCTCG+AGG 0.689356 3.4:-93224242 MS.gene67063:CDS
GTTGGCACACAATCTCGAGG+AGG 0.726557 3.4:-93225603 MS.gene67063:CDS
TGGGCTGGTATCGTTCACCA+AGG 0.727978 3.4:-93224896 MS.gene67063:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GTCAATTTAATTTTTTTGTT+TGG - chr3.4:93224647-93224666 MS.gene67063:CDS 15.0%
!!! TATGATTTCTATTTTAGTTT+TGG - chr3.4:93224320-93224339 MS.gene67063:CDS 15.0%
!!! TTTTGAGATAGTATATAATT+TGG - chr3.4:93224403-93224422 MS.gene67063:CDS 15.0%
!! TTATATAATACTCATTCATG+TGG - chr3.4:93224694-93224713 MS.gene67063:CDS 20.0%
!!! AATTAAATCTACTTTTTTGG+TGG - chr3.4:93224344-93224363 MS.gene67063:CDS 20.0%
!!! ACGATAAAAGATAATTTTGT+TGG - chr3.4:93224458-93224477 MS.gene67063:CDS 20.0%
!!! AGTTGTTATATGTATTTTGT+GGG - chr3.4:93224482-93224501 MS.gene67063:CDS 20.0%
!!! GGTAATTAAATCTACTTTTT+TGG - chr3.4:93224341-93224360 MS.gene67063:CDS 20.0%
!!! TAGTTGTTATATGTATTTTG+TGG - chr3.4:93224481-93224500 MS.gene67063:CDS 20.0%
! AAACCTTAATGGTTGTAAAA+AGG - chr3.4:93226372-93226391 MS.gene67063:intron 25.0%
! TTCATGTTTCATCTGATTAT+TGG - chr3.4:93224195-93224214 MS.gene67063:CDS 25.0%
!! ATATGTCTAGTTATGTGTTT+TGG - chr3.4:93224625-93224644 MS.gene67063:CDS 25.0%
!! TTTTATATTTAATCCCTCGA+GGG + chr3.4:93226596-93226615 None:intergenic 25.0%
!! TTTTTATATTTAATCCCTCG+AGG + chr3.4:93226597-93226616 None:intergenic 25.0%
!!! AGGACAGTTTTTAATTGATA+CGG + chr3.4:93225573-93225592 None:intergenic 25.0%
!!! TGTCAGCTTTTTTAGAATAA+TGG + chr3.4:93225639-93225658 None:intergenic 25.0%
!!! TTGTCTGCTTTGAAAAAATT+GGG - chr3.4:93226565-93226584 MS.gene67063:intron 25.0%
CTTAATGGTTGTAAAAAGGT+CGG - chr3.4:93226376-93226395 MS.gene67063:intron 30.0%
GTATCAATTAAAAACTGTCC+TGG - chr3.4:93225572-93225591 MS.gene67063:CDS 30.0%
TAGATTAACATTACCCAAAC+TGG + chr3.4:93226278-93226297 None:intergenic 30.0%
TGCTCAAAATTCATTTGTGT+TGG - chr3.4:93224593-93224612 MS.gene67063:CDS 30.0%
TTAATGGTTGTAAAAAGGTC+GGG - chr3.4:93226377-93226396 MS.gene67063:intron 30.0%
TTGGGTAATGTTAATCTAAG+TGG - chr3.4:93226280-93226299 MS.gene67063:intron 30.0%
! AGCTGAAAGTTTTTCACATT+TGG + chr3.4:93225878-93225897 None:intergenic 30.0%
! TAATGGATGCTGAACATAAT+AGG + chr3.4:93225622-93225641 None:intergenic 30.0%
!! AATCAAGAATTTGGTGATGA+AGG - chr3.4:93225134-93225153 MS.gene67063:CDS 30.0%
!! CTTGTCTGCTTTGAAAAAAT+TGG - chr3.4:93226564-93226583 MS.gene67063:intron 30.0%
AAAATTGAAGGCTCTTACTC+TGG - chr3.4:93226036-93226055 MS.gene67063:intron 35.0%
AAAGTACATCCAGATTGAAC+AGG + chr3.4:93226510-93226529 None:intergenic 35.0%
AAATTGTTGGCAACTTGCAT+AGG + chr3.4:93225424-93225443 None:intergenic 35.0%
AATGAAGGTCTACATGCTAT+TGG - chr3.4:93225524-93225543 MS.gene67063:CDS 35.0%
AATGACGAAATTGTCTGACA+TGG + chr3.4:93226112-93226131 None:intergenic 35.0%
AATTCAAGACACTGAAGCAT+AGG + chr3.4:93226196-93226215 None:intergenic 35.0%
AATTGTAGGCTCACAATTGA+TGG + chr3.4:93225956-93225975 None:intergenic 35.0%
AGAGTAAAATTACCAACTCC+AGG + chr3.4:93226151-93226170 None:intergenic 35.0%
ATCAATTGTGAGCCTACAAT+TGG - chr3.4:93225955-93225974 MS.gene67063:CDS 35.0%
ATCGATAACTCAGTCAGATT+CGG + chr3.4:93225482-93225501 None:intergenic 35.0%
ATTGATACGGACCTTAGATT+TGG + chr3.4:93225560-93225579 None:intergenic 35.0%
CAATCTGGATGTACTTTCTT+TGG - chr3.4:93226513-93226532 MS.gene67063:intron 35.0%
CTAGCAGTTATTGGACAATA+TGG - chr3.4:93225689-93225708 MS.gene67063:CDS 35.0%
CTTCAGTGTCTTGAATTGAT+TGG - chr3.4:93226199-93226218 MS.gene67063:intron 35.0%
CTTGAATTGATTGGACTTGA+GGG - chr3.4:93226208-93226227 MS.gene67063:intron 35.0%
GAAAGTTGTGTCCAAATCTA+AGG - chr3.4:93225546-93225565 MS.gene67063:CDS 35.0%
GAACCAAATCAAAAGCTTAC+CGG + chr3.4:93224178-93224197 None:intergenic 35.0%
GAGTAAAATTACCAACTCCA+GGG + chr3.4:93226150-93226169 None:intergenic 35.0%
GATTACTCACTAGCAGTTAT+TGG - chr3.4:93225680-93225699 MS.gene67063:CDS 35.0%
GTCTGTAAGCATATTCTTGA+CGG + chr3.4:93224866-93224885 None:intergenic 35.0%
TAATGGTTGTAAAAAGGTCG+GGG - chr3.4:93226378-93226397 MS.gene67063:intron 35.0%
TAATGGTTGTCATCAGATAG+AGG - chr3.4:93225391-93225410 MS.gene67063:CDS 35.0%
TCTTGAATTGATTGGACTTG+AGG - chr3.4:93226207-93226226 MS.gene67063:intron 35.0%
TGAAAAACTTTCAGCTTCGT+AGG - chr3.4:93225882-93225901 MS.gene67063:CDS 35.0%
TGAGAAATCAAACACACACA+AGG + chr3.4:93224769-93224788 None:intergenic 35.0%
TTAGGGAAGCTTTCAATTCA+TGG - chr3.4:93225242-93225261 MS.gene67063:CDS 35.0%
TTGACTTCAGTGGAGAAAAA+TGG - chr3.4:93224919-93224938 MS.gene67063:CDS 35.0%
TTTCTCCACTGAAGTCAAAT+GGG + chr3.4:93224917-93224936 None:intergenic 35.0%
! AAAGCCTTGTCAGAAATTGT+TGG + chr3.4:93225437-93225456 None:intergenic 35.0%
! AATTTTACTCTCAACGTCCT+TGG - chr3.4:93226160-93226179 MS.gene67063:intron 35.0%
! ATTTTACTCTCAACGTCCTT+GGG - chr3.4:93226161-93226180 MS.gene67063:intron 35.0%
! CGACCTTTTTACAACCATTA+AGG + chr3.4:93226378-93226397 None:intergenic 35.0%
! GTGATGATTTTTGTACTGGA+AGG - chr3.4:93224793-93224812 MS.gene67063:CDS 35.0%
! TATTGGACAATATGGCTTTG+TGG - chr3.4:93225697-93225716 MS.gene67063:CDS 35.0%
! TCTAAAGTTCTCAGCTTTAC+CGG - chr3.4:93224156-93224175 MS.gene67063:CDS 35.0%
! TCTGAAGTCTTTCACTCTTT+GGG - chr3.4:93225328-93225347 MS.gene67063:CDS 35.0%
! TTGTGTGTGTTTGATTTCTC+AGG - chr3.4:93224768-93224787 MS.gene67063:CDS 35.0%
! TTTTCTCCACTGAAGTCAAA+TGG + chr3.4:93224918-93224937 None:intergenic 35.0%
!! ACTCATTCATGTGGTTTTAC+TGG - chr3.4:93224703-93224722 MS.gene67063:CDS 35.0%
!! TTACCGGTAAGCTTTTGATT+TGG - chr3.4:93224172-93224191 MS.gene67063:CDS 35.0%
AAACCGTGGTGCAAAATTGA+AGG - chr3.4:93226024-93226043 MS.gene67063:CDS 40.0%
AACTAACGTCGCTCACAATT+GGG - chr3.4:93225801-93225820 MS.gene67063:CDS 40.0%
AAGTTGCATTGTTCGACTCT+TGG - chr3.4:93226343-93226362 MS.gene67063:intron 40.0%
AATCCTTTCTCGGTGACATT+TGG + chr3.4:93225749-93225768 None:intergenic 40.0%
AGAGTGAAAGACTTCAGAGA+AGG + chr3.4:93225326-93225345 None:intergenic 40.0%
ATAGAGTCATGTCCTAGCAT+CGG - chr3.4:93225500-93225519 MS.gene67063:CDS 40.0%
ATTGTCTGCAATTGCTGTCA+AGG + chr3.4:93226410-93226429 None:intergenic 40.0%
CCATAGGATTACCCAATTTG+GGG - chr3.4:93225985-93226004 MS.gene67063:CDS 40.0%
GAAGCAGTGAAATAAACCCT+GGG + chr3.4:93226243-93226262 None:intergenic 40.0%
GACGGTGACAATCAAGAATT+TGG - chr3.4:93225125-93225144 MS.gene67063:CDS 40.0%
GAGTGAAAGACTTCAGAGAA+GGG + chr3.4:93225325-93225344 None:intergenic 40.0%
GATCAAGCCTTTGTCACTAA+TGG + chr3.4:93225363-93225382 None:intergenic 40.0%
GCCATAGGATTACCCAATTT+GGG - chr3.4:93225984-93226003 MS.gene67063:CDS 40.0%
GGTTTATTTCACTGCTTCAG+AGG - chr3.4:93226245-93226264 MS.gene67063:intron 40.0%
GTGCATTTCTGTCAGACAAT+GGG - chr3.4:93225906-93225925 MS.gene67063:CDS 40.0%
GTTGCCAACAATTTCTGACA+AGG - chr3.4:93225430-93225449 MS.gene67063:CDS 40.0%
GTTGCTACCATTAGTGACAA+AGG - chr3.4:93225353-93225372 MS.gene67063:CDS 40.0%
GTTGCTGCAGATTTCATTCT+TGG + chr3.4:93225087-93225106 None:intergenic 40.0%
TATCTTTCTCGAAGCTTGGA+GGG - chr3.4:93225158-93225177 MS.gene67063:CDS 40.0%
TATGGCACTCTTCCAATTGT+AGG + chr3.4:93225970-93225989 None:intergenic 40.0%
TGAAGCAGTGAAATAAACCC+TGG + chr3.4:93226244-93226263 None:intergenic 40.0%
TGCATCAATCTGACAGATGT+AGG - chr3.4:93226304-93226323 MS.gene67063:intron 40.0%
TGCCATAGGATTACCCAATT+TGG - chr3.4:93225983-93226002 MS.gene67063:CDS 40.0%
TGTGCATTTCTGTCAGACAA+TGG - chr3.4:93225905-93225924 MS.gene67063:CDS 40.0%
TTTCTCGGTGACATTTGGAA+GGG + chr3.4:93225744-93225763 None:intergenic 40.0%
! AATGTCCCATTTGACTTCAG+TGG - chr3.4:93224909-93224928 MS.gene67063:CDS 40.0%
! ACCACGGTTTAAAATGACAC+CGG + chr3.4:93226014-93226033 None:intergenic 40.0%
! ACCTTCTAAGGATCTCAAAG+AGG + chr3.4:93224992-93225011 None:intergenic 40.0%
! CTTGGAGTGCTAAACCTTAA+TGG - chr3.4:93226361-93226380 MS.gene67063:intron 40.0%
! GGAGGTGATGATTTTTGTAC+TGG - chr3.4:93224789-93224808 MS.gene67063:CDS 40.0%
! TGTGTGTTTGATTTCTCAGG+AGG - chr3.4:93224771-93224790 MS.gene67063:CDS 40.0%
!! AAGCGAGATCTCTTTTGAAG+AGG + chr3.4:93224888-93224907 None:intergenic 40.0%
!! AGATGTAGGAGTTTTGTCCA+TGG - chr3.4:93226318-93226337 MS.gene67063:intron 40.0%
!! ATCTCAAAGAGGCATTCATC+AGG + chr3.4:93224981-93225000 None:intergenic 40.0%
!! CTCTGAAGTCTTTCACTCTT+TGG - chr3.4:93225327-93225346 MS.gene67063:CDS 40.0%
!! TACAATTGGAAGAGTGCCAT+AGG - chr3.4:93225969-93225988 MS.gene67063:CDS 40.0%
AAAATTGGGTGATTCCCTCG+AGG - chr3.4:93226579-93226598 MS.gene67063:intron 45.0%
AAATTGGGTGATTCCCTCGA+GGG - chr3.4:93226580-93226599 MS.gene67063:intron 45.0%
AAGCTCCTATCTTGTCCTTC+AGG + chr3.4:93225017-93225036 None:intergenic 45.0%
ACACAATCTCGAGGAGGATT+AGG - chr3.4:93225224-93225243 MS.gene67063:CDS 45.0%
AGACCTTCATTACCGATGCT+AGG + chr3.4:93225515-93225534 None:intergenic 45.0%
AGATTGTGTGCCAACAGCAA+TGG + chr3.4:93225213-93225232 None:intergenic 45.0%
AGCCCAATATCTGTTACTCC+AGG + chr3.4:93225833-93225852 None:intergenic 45.0%
AGGCTATCTTTCTCGAAGCT+TGG - chr3.4:93225154-93225173 MS.gene67063:CDS 45.0%
ATCCTTAGAAGGTTGCCTGA+AGG - chr3.4:93224999-93225018 MS.gene67063:CDS 45.0%
ATCTTTCTCGAAGCTTGGAG+GGG - chr3.4:93225159-93225178 MS.gene67063:CDS 45.0%
ATGTCACCGAGAAAGGATTC+TGG - chr3.4:93225750-93225769 MS.gene67063:CDS 45.0%
ATTTCTGTCAGACAATGGGC+TGG - chr3.4:93225910-93225929 MS.gene67063:CDS 45.0%
CAACTAACGTCGCTCACAAT+TGG - chr3.4:93225800-93225819 MS.gene67063:CDS 45.0%
CACAATCTCGAGGAGGATTA+GGG - chr3.4:93225225-93225244 MS.gene67063:CDS 45.0%
CATCACCCAGAATCCTTTCT+CGG + chr3.4:93225759-93225778 None:intergenic 45.0%
CCCCAAATTGGGTAATCCTA+TGG + chr3.4:93225988-93226007 None:intergenic 45.0%
CGAACAATGCAACTTGACCA+TGG + chr3.4:93226338-93226357 None:intergenic 45.0%
CTATCTTTCTCGAAGCTTGG+AGG - chr3.4:93225157-93225176 MS.gene67063:CDS 45.0%
CTGGATGTACTTTCTTTGGC+AGG - chr3.4:93226517-93226536 MS.gene67063:intron 45.0%
CTTACTCTGGTAAGCTGCTA+CGG - chr3.4:93226049-93226068 MS.gene67063:intron 45.0%
CTTCCAAATGTCACCGAGAA+AGG - chr3.4:93225743-93225762 MS.gene67063:CDS 45.0%
CTTGACTGATGTAGGTCTCA+AGG - chr3.4:93225283-93225302 MS.gene67063:CDS 45.0%
CTTGAGACCTACATCAGTCA+AGG + chr3.4:93225285-93225304 None:intergenic 45.0%
CTTTCTCGGTGACATTTGGA+AGG + chr3.4:93225745-93225764 None:intergenic 45.0%
GAGAAAGGATTCTGGGTGAT+GGG - chr3.4:93225758-93225777 MS.gene67063:CDS 45.0%
GATATTGGGCTTGAAGCTGT+AGG - chr3.4:93225842-93225861 MS.gene67063:CDS 45.0%
GCCCAATATCTGTTACTCCA+GGG + chr3.4:93225832-93225851 None:intergenic 45.0%
GCCCTGGAGTAACAGATATT+GGG - chr3.4:93225828-93225847 MS.gene67063:CDS 45.0%
GGCTTGATCGAGATTGCTAA+TGG - chr3.4:93225374-93225393 MS.gene67063:CDS 45.0%
TGCCCTGGAGTAACAGATAT+TGG - chr3.4:93225827-93225846 MS.gene67063:CDS 45.0%
TGTCACCGAGAAAGGATTCT+GGG - chr3.4:93225751-93225770 MS.gene67063:CDS 45.0%
TTACTCTGGTAAGCTGCTAC+GGG - chr3.4:93226050-93226069 MS.gene67063:intron 45.0%
TTATCAATCCGTAACTGCCC+TGG - chr3.4:93226130-93226149 MS.gene67063:intron 45.0%
TTCACTGCTTCAGAGGTCTA+AGG - chr3.4:93226252-93226271 MS.gene67063:intron 45.0%
TTCTTTGGCAGGTTGCTCAT+TGG - chr3.4:93226528-93226547 MS.gene67063:intron 45.0%
TTGAGGGAATAACAGACCCA+GGG - chr3.4:93226224-93226243 MS.gene67063:intron 45.0%
! ATTACCCAATTTGGGGTTGC+CGG - chr3.4:93225992-93226011 MS.gene67063:CDS 45.0%
! GAGCCTTCAATTTTGCACCA+CGG + chr3.4:93226030-93226049 None:intergenic 45.0%
!! CAACGCAAGTTCTGGAAACA+AGG - chr3.4:93225103-93225122 MS.gene67063:CDS 45.0%
!! CCACAAGCTTGTTAACTGTG+CGG + chr3.4:93226639-93226658 None:intergenic 45.0%
!! GCAAGTTCTGGAAACAAGGA+CGG - chr3.4:93225107-93225126 MS.gene67063:CDS 45.0%
!! GCCTCTTTGAGATCCTTAGA+AGG - chr3.4:93224988-93225007 MS.gene67063:CDS 45.0%
!! TGTCCTAGCATCGGTAATGA+AGG - chr3.4:93225509-93225528 MS.gene67063:CDS 45.0%
AAGGGCAGAAATCCCTGCAT+CGG + chr3.4:93226476-93226495 None:intergenic 50.0%
ACAAGCTTGTGGAACGTCTC+TGG - chr3.4:93226647-93226666 MS.gene67063:intron 50.0%
ACATCAGTCAAGGCACGATC+TGG + chr3.4:93225275-93225294 None:intergenic 50.0%
ACGTTAGTTGCTGCAGTGCA+TGG + chr3.4:93225790-93225809 None:intergenic 50.0%
AGTGTGCAATCACCGATGCA+GGG - chr3.4:93226461-93226480 MS.gene67063:intron 50.0%
ATCTGCAGCAACGCAAGTTC+TGG - chr3.4:93225095-93225114 MS.gene67063:CDS 50.0%
ATTCCCTGATCACGAACTCC+AGG + chr3.4:93225593-93225612 None:intergenic 50.0%
CACAATTGATGGAGCAGCCT+TGG + chr3.4:93225945-93225964 None:intergenic 50.0%
CCGCACAGTTAACAAGCTTG+TGG - chr3.4:93226636-93226655 MS.gene67063:intron 50.0%
CGAGAAAGGATTCTGGGTGA+TGG - chr3.4:93225757-93225776 MS.gene67063:CDS 50.0%
CTTGAGGGAATAACAGACCC+AGG - chr3.4:93226223-93226242 MS.gene67063:intron 50.0%
GAACAGGACTCCACGAGTAA+GGG + chr3.4:93226494-93226513 None:intergenic 50.0%
GATCGTGCCTTGACTGATGT+AGG - chr3.4:93225275-93225294 MS.gene67063:CDS 50.0%
GATGTACCTTAACCCGAAGG+AGG - chr3.4:93224818-93224837 MS.gene67063:CDS 50.0%
GGGCTTGAAGCTGTAGGAAA+GGG - chr3.4:93225848-93225867 MS.gene67063:CDS 50.0%
GGTTGCCTGAAGGACAAGAT+AGG - chr3.4:93225009-93225028 MS.gene67063:CDS 50.0%
GTCCTTCAGGCAACCTTCTA+AGG + chr3.4:93225004-93225023 None:intergenic 50.0%
GTCGATGTACCTTAACCCGA+AGG - chr3.4:93224815-93224834 MS.gene67063:CDS 50.0%
GTCTGACATGGAGATACTGC+TGG + chr3.4:93226100-93226119 None:intergenic 50.0%
GTTCGTGATCAGGGAATTGC+TGG - chr3.4:93225596-93225615 MS.gene67063:CDS 50.0%
TACCAACTCCAGGGCAGTTA+CGG + chr3.4:93226141-93226160 None:intergenic 50.0%
TCGAAGCTTGGAGGGGAAAA+AGG - chr3.4:93225166-93225185 MS.gene67063:CDS 50.0%
TCGTGGAGTCCTGTTCAATC+TGG - chr3.4:93226498-93226517 MS.gene67063:intron 50.0%
TGAACAGGACTCCACGAGTA+AGG + chr3.4:93226495-93226514 None:intergenic 50.0%
TGGGCTTGAAGCTGTAGGAA+AGG - chr3.4:93225847-93225866 MS.gene67063:CDS 50.0%
TGTCCTGGAGTTCGTGATCA+GGG - chr3.4:93225587-93225606 MS.gene67063:CDS 50.0%
! AGACTAGCTGCCATTGCTGT+TGG - chr3.4:93225200-93225219 MS.gene67063:CDS 50.0%
! CAGAGGTCTAAGGCCAGTTT+GGG - chr3.4:93226262-93226281 MS.gene67063:intron 50.0%
! TCAGAGGTCTAAGGCCAGTT+TGG - chr3.4:93226261-93226280 MS.gene67063:intron 50.0%
! TGCATCGGTGATTGCACACT+CGG + chr3.4:93226461-93226480 None:intergenic 50.0%
!! GGATTCTGGGTGATGGGTAA+TGG - chr3.4:93225764-93225783 MS.gene67063:CDS 50.0%
!!! GCCGGTGTCATTTTAAACCG+TGG - chr3.4:93226010-93226029 MS.gene67063:CDS 50.0%
AAGAAGAGACCCGCCTCCTT+CGG + chr3.4:93224834-93224853 None:intergenic 55.0%
ATCCGTAACTGCCCTGGAGT+TGG - chr3.4:93226136-93226155 MS.gene67063:intron 55.0%
CTGTCCTGGAGTTCGTGATC+AGG - chr3.4:93225586-93225605 MS.gene67063:CDS 55.0%
GACACCGGCAACCCCAAATT+GGG + chr3.4:93225999-93226018 None:intergenic 55.0%
GAGTGTGCAATCACCGATGC+AGG - chr3.4:93226460-93226479 MS.gene67063:intron 55.0%
GCATAGGACAGAGCTTCCCA+AGG + chr3.4:93226180-93226199 None:intergenic 55.0%
GCGGGTCTCTTCTTGTCTCT+TGG - chr3.4:93224840-93224859 MS.gene67063:CDS 55.0%
GCTGTTGGCACACAATCTCG+AGG - chr3.4:93225215-93225234 MS.gene67063:CDS 55.0%
GGGATTTCTGCCCTTACTCG+TGG - chr3.4:93226481-93226500 MS.gene67063:intron 55.0%
GGTCTCAAGGCAGTTGCTCA+TGG - chr3.4:93225296-93225315 MS.gene67063:CDS 55.0%
GTACCTTAACCCGAAGGAGG+CGG - chr3.4:93224821-93224840 MS.gene67063:CDS 55.0%
GTGCTTCTGTATCCAAGCGC+TGG - chr3.4:93225039-93225058 MS.gene67063:CDS 55.0%
GTTGGCACACAATCTCGAGG+AGG - chr3.4:93225218-93225237 MS.gene67063:CDS 55.0%
TACCTTAACCCGAAGGAGGC+GGG - chr3.4:93224822-93224841 MS.gene67063:CDS 55.0%
TGACACCGGCAACCCCAAAT+TGG + chr3.4:93226000-93226019 None:intergenic 55.0%
!! TGGGCTGGTATCGTTCACCA+AGG - chr3.4:93225925-93225944 MS.gene67063:CDS 55.0%
AGAAGAGACCCGCCTCCTTC+GGG + chr3.4:93224833-93224852 None:intergenic 60.0%
CTCACAATTGGGCTGTGCCC+TGG - chr3.4:93225812-93225831 MS.gene67063:CDS 60.0%
! TAGAAGCGTGAGCCAGCGCT+TGG + chr3.4:93225054-93225073 None:intergenic 60.0%
! GACCCGCCTCCTTCGGGTTA+AGG + chr3.4:93224827-93224846 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr3.4 gene 93224153 93226690 93224153 ID=MS.gene67063
chr3.4 mRNA 93224153 93226690 93224153 ID=MS.gene67063.t1;Parent=MS.gene67063
chr3.4 exon 93226666 93226690 93226666 ID=MS.gene67063.t1.exon1;Parent=MS.gene67063.t1
chr3.4 CDS 93226666 93226690 93226666 ID=cds.MS.gene67063.t1;Parent=MS.gene67063.t1
chr3.4 exon 93224153 93226053 93224153 ID=MS.gene67063.t1.exon2;Parent=MS.gene67063.t1
chr3.4 CDS 93224153 93226053 93224153 ID=cds.MS.gene67063.t1;Parent=MS.gene67063.t1
Gene Sequence

>MS.gene67063

ATGTCTAAAGTTCTCAGCTTTACCGGTAAGCTTTTGATTTGGTTCATGTTTCATCTGATTATTGGTTTTAGCTGTTGAGTTTTGATTCTGTTATTTTATAAAGTTTGTTATCATAGATATGAATTTGATGTTTTGATTTTAGTTTTCTGTGTTCTATATTGATTTGTTATGATTTCTATTTTAGTTTTGGTAATTAAATCTACTTTTTTGGTGGATCTGATTAGTGATATAGACAATTTAATTTTATTTATTTTGAGATAGTATATAATTTGGCATGAAATTGTTTTAATAATTTGCTTTTAGTGACGATAAAAGATAATTTTGTTGGTAGTTGTTATATGTATTTTGTGGGAAATGAATCATGTTCACATATATGATAGATAGTTTTGTATGCATATGAGATCAATAATTTTGCTCAAAATTGATTTGATCAGTAATTTTGCTCAAAATTCATTTGTGTTGGATTTCTTTAATATGTCTAGTTATGTGTTTTGGTCAATTTAATTTTTTTGTTTGGTTGTGTTCAAATCTTTTACTCAGATTATATAATACTCATTCATGTGGTTTTACTGGTAGAGTTTTTATGTTTCTCTAATTTTATTTTTTTTATTTACCTTGTGTGTGTTTGATTTCTCAGGAGGTGATGATTTTTGTACTGGAAGGTCGATGTACCTTAACCCGAAGGAGGCGGGTCTCTTCTTGTCTCTTGGCCGTCAAGAATATGCTTACAGACCTCTTCAAAAGAGATCTCGCTTCAATGTCCCATTTGACTTCAGTGGAGAAAAATGGTTTGAGCAGAAGCTAGAGACATCTATTGAAACATTGCCTGATGAATGCCTCTTTGAGATCCTTAGAAGGTTGCCTGAAGGACAAGATAGGAGCTTATGTGCTTCTGTATCCAAGCGCTGGCTCACGCTTCTAAGCAGTATTTCCAAGAATGAAATCTGCAGCAACGCAAGTTCTGGAAACAAGGACGGTGACAATCAAGAATTTGGTGATGAAGGCTATCTTTCTCGAAGCTTGGAGGGGAAAAAGGCAACAGATGTCAGACTAGCTGCCATTGCTGTTGGCACACAATCTCGAGGAGGATTAGGGAAGCTTTCAATTCATGGAAGCAATCCAGATCGTGCCTTGACTGATGTAGGTCTCAAGGCAGTTGCTCATGGCTGCCCTTCTCTGAAGTCTTTCACTCTTTGGGATGTTGCTACCATTAGTGACAAAGGCTTGATCGAGATTGCTAATGGTTGTCATCAGATAGAGGAGCTTGACCTATGCAAGTTGCCAACAATTTCTGACAAGGCTTTGATTGCAGTTGCAAAGCACTGTCCGAATCTGACTGAGTTATCGATAGAGTCATGTCCTAGCATCGGTAATGAAGGTCTACATGCTATTGGAAAGTTGTGTCCAAATCTAAGGTCCGTATCAATTAAAAACTGTCCTGGAGTTCGTGATCAGGGAATTGCTGGCCTATTATGTTCAGCATCCATTATTCTAAAAAAGCTGACACTTGAATCGCTAGCTGTTTCTGATTACTCACTAGCAGTTATTGGACAATATGGCTTTGTGGTTACTGATCTTGTCTTAAATTCCCTTCCAAATGTCACCGAGAAAGGATTCTGGGTGATGGGTAATGGCCATGCACTGCAGCAACTAACGTCGCTCACAATTGGGCTGTGCCCTGGAGTAACAGATATTGGGCTTGAAGCTGTAGGAAAGGGTTGTCCAAATGTGAAAAACTTTCAGCTTCGTAGGTGTGCATTTCTGTCAGACAATGGGCTGGTATCGTTCACCAAGGCTGCTCCATCAATTGTGAGCCTACAATTGGAAGAGTGCCATAGGATTACCCAATTTGGGGTTGCCGGTGTCATTTTAAACCGTGGTGCAAAATTGAAGGCTCTTACTCTGGTAAGCTGCTACGGGATCAAAGATCTGAATCTCAATTTGCCAGCAGTATCTCCATGTCAGACAATTTCGTCATTATCAATCCGTAACTGCCCTGGAGTTGGTAATTTTACTCTCAACGTCCTTGGGAAGCTCTGTCCTATGCTTCAGTGTCTTGAATTGATTGGACTTGAGGGAATAACAGACCCAGGGTTTATTTCACTGCTTCAGAGGTCTAAGGCCAGTTTGGGTAATGTTAATCTAAGTGGTTGCATCAATCTGACAGATGTAGGAGTTTTGTCCATGGTCAAGTTGCATTGTTCGACTCTTGGAGTGCTAAACCTTAATGGTTGTAAAAAGGTCGGGGATGCTTCCTTGACAGCAATTGCAGACAATTGCATTGTCTTGAGTGATCTTGATGTTTCCGAGTGTGCAATCACCGATGCAGGGATTTCTGCCCTTACTCGTGGAGTCCTGTTCAATCTGGATGTACTTTCTTTGGCAGGTTGCTCATTGGTTTCAAACAAGAGCTTGTCTGCTTTGAAAAAATTGGGTGATTCCCTCGAGGGATTAAATATAAAAAATTGCAATTCGATCAGCAGCCGCACAGTTAACAAGCTTGTGGAACGTCTCTGGATGTGTGACATCCTCTTCTGA
Protein sequence

>MS.gene67063.t1

MSKVLSFTGGDDFCTGRSMYLNPKEAGLFLSLGRQEYAYRPLQKRSRFNVPFDFSGEKWFEQKLETSIETLPDECLFEILRRLPEGQDRSLCASVSKRWLTLLSSISKNEICSNASSGNKDGDNQEFGDEGYLSRSLEGKKATDVRLAAIAVGTQSRGGLGKLSIHGSNPDRALTDVGLKAVAHGCPSLKSFTLWDVATISDKGLIEIANGCHQIEELDLCKLPTISDKALIAVAKHCPNLTELSIESCPSIGNEGLHAIGKLCPNLRSVSIKNCPGVRDQGIAGLLCSASIILKKLTLESLAVSDYSLAVIGQYGFVVTDLVLNSLPNVTEKGFWVMGNGHALQQLTSLTIGLCPGVTDIGLEAVGKGCPNVKNFQLRRCAFLSDNGLVSFTKAAPSIVSLQLEECHRITQFGVAGVILNRGAKLKALTLVSCYGIKDLNLNLPAVSPCQTISSLSIRNCPGVGNFTLNVLGKLCPMLQCLELIGLEGITDPGFISLLQRSKASLGNVNLSGCINLTDVGVLSMVKLHCSTLGVLNLNGCKKVGDASLTAIADNCIVLSDLDVSECAITDAGISALTRGVLFNLDVLSLAGCSLVSNKSLSALKKLGDSLEGLNIKNCNSISSRTVNKLVERLWMCDILF