Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67242.t1 | XP_003615940.1 | 88.8 | 160 | 18 | 0 | 1 | 160 | 1 | 160 | 2.30E-67 | 265 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67242.t1 | G7K5S3 | 88.8 | 160 | 18 | 0 | 1 | 160 | 1 | 160 | 1.6e-67 | 265.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050945 | MS.gene67242 | 0.836089 | 1.15E-56 | -1.69E-46 |
| MS.gene054004 | MS.gene67242 | 0.806178 | 9.33E-50 | -1.69E-46 |
| MS.gene054413 | MS.gene67242 | 0.810735 | 9.94E-51 | -1.69E-46 |
| MS.gene055150 | MS.gene67242 | 0.814638 | 1.39E-51 | -1.69E-46 |
| MS.gene055886 | MS.gene67242 | 0.815424 | 9.31E-52 | -1.69E-46 |
| MS.gene060244 | MS.gene67242 | 0.88033 | 6.00E-70 | -1.69E-46 |
| MS.gene060975 | MS.gene67242 | 0.81461 | 1.41E-51 | -1.69E-46 |
| MS.gene060977 | MS.gene67242 | 0.824984 | 6.00E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene67242 | MS.gene002580 | PPI |
| MS.gene004251 | MS.gene67242 | PPI |
| MS.gene67242 | MS.gene01564 | PPI |
| MS.gene54297 | MS.gene67242 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67242.t1 | MTR_5g074290 | 98.125 | 160 | 3 | 0 | 1 | 160 | 1 | 160 | 1.21e-112 | 316 |
| MS.gene67242.t1 | MTR_3g031140 | 65.116 | 172 | 44 | 4 | 1 | 158 | 1 | 170 | 2.44e-67 | 202 |
| MS.gene67242.t1 | MTR_2g016150 | 42.778 | 180 | 80 | 5 | 1 | 157 | 1 | 180 | 7.64e-26 | 97.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67242.t1 | AT2G30230 | 56.425 | 179 | 55 | 4 | 1 | 159 | 1 | 176 | 4.75e-58 | 179 |
| MS.gene67242.t1 | AT1G06980 | 59.302 | 172 | 53 | 4 | 1 | 159 | 1 | 168 | 8.55e-58 | 178 |
| MS.gene67242.t1 | AT1G29195 | 39.683 | 189 | 81 | 5 | 1 | 157 | 1 | 188 | 1.78e-34 | 119 |
Find 32 sgRNAs with CRISPR-Local
Find 36 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATCCGTATAGTTCATTTAAA+TGG | 0.184956 | 5.1:+56972584 | MS.gene67242:CDS |
| AGAACTTCCCCAGCTGAAAT+TGG | 0.250090 | 5.1:-56972629 | None:intergenic |
| GAATAGCTTAAGGTGTTGTT+TGG | 0.353238 | 5.1:+56972529 | MS.gene67242:CDS |
| GATCCCATCCACGTCGTTAC+CGG | 0.355538 | 5.1:+56972763 | MS.gene67242:CDS |
| GGTTTGCTTAGAACATGATT+TGG | 0.371952 | 5.1:-56972662 | None:intergenic |
| CGAACGACTCCTTCAGAGCT+TGG | 0.393027 | 5.1:-56972683 | None:intergenic |
| ACAGAAACATTGTGAGTCTA+AGG | 0.406561 | 5.1:+56972901 | MS.gene67242:CDS |
| AGCCATTTAAATGAACTATA+CGG | 0.418439 | 5.1:-56972586 | None:intergenic |
| GCTTAGAACATGATTTGGAT+TGG | 0.460869 | 5.1:-56972657 | None:intergenic |
| CAATAATGGGGAATAGCTTA+AGG | 0.463109 | 5.1:+56972519 | None:intergenic |
| TTCTCCGGTAACGACGTGGA+TGG | 0.466777 | 5.1:-56972767 | None:intergenic |
| ATTACACGTCCAATTTCAGC+TGG | 0.481683 | 5.1:+56972620 | MS.gene67242:CDS |
| TCTCCGGTAACGACGTGGAT+GGG | 0.485747 | 5.1:-56972766 | None:intergenic |
| ATCGCCGGCATAGCCGTAGC+TGG | 0.494180 | 5.1:+56972945 | MS.gene67242:CDS |
| CAAGATGAGGTTGCCAGCTA+CGG | 0.497711 | 5.1:-56972958 | None:intergenic |
| GTTGCCAGCTACGGCTATGC+CGG | 0.501915 | 5.1:-56972949 | None:intergenic |
| CTAAGCAAACCAAGCTCTGA+AGG | 0.503856 | 5.1:+56972674 | MS.gene67242:CDS |
| CCGGCGAAAGGATGAGAATC+CGG | 0.510917 | 5.1:-56972706 | None:intergenic |
| CTTCTTGCTCTTCTCTTCAG+AGG | 0.524828 | 5.1:-56972852 | None:intergenic |
| CCGGATTCTCATCCTTTCGC+CGG | 0.527436 | 5.1:+56972706 | MS.gene67242:CDS |
| AAAGCTCGAGGCGAGATCGC+CGG | 0.545398 | 5.1:+56972930 | MS.gene67242:CDS |
| TCTATGATACTTTCAAGATG+AGG | 0.552754 | 5.1:-56972971 | None:intergenic |
| GCTCTGAAGGAGTCGTTCGC+CGG | 0.557154 | 5.1:+56972687 | MS.gene67242:CDS |
| TGAGCTCAGTCTCCGGCGAA+AGG | 0.557541 | 5.1:-56972718 | None:intergenic |
| CCTCTCTTGAGCTCAGTCTC+CGG | 0.558760 | 5.1:-56972725 | None:intergenic |
| TTGGCTTGTGTTCTTCCATG+TGG | 0.602379 | 5.1:+56972548 | MS.gene67242:CDS |
| CCGGAGACTGAGCTCAAGAG+AGG | 0.612896 | 5.1:+56972725 | MS.gene67242:CDS |
| TACACGTCCAATTTCAGCTG+GGG | 0.613892 | 5.1:+56972622 | MS.gene67242:CDS |
| TTACACGTCCAATTTCAGCT+GGG | 0.627095 | 5.1:+56972621 | MS.gene67242:CDS |
| ATCAAGTCTAGTGCTCCACA+TGG | 0.636272 | 5.1:-56972563 | None:intergenic |
| CTAAGGAGATGAAAAGCTCG+AGG | 0.669226 | 5.1:+56972918 | MS.gene67242:CDS |
| GAGGGTCAGAAGAAGCGTCG+CGG | 0.726452 | 5.1:+56972796 | MS.gene67242:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AGCCATTTAAATGAACTATA+CGG | - | chr5.1:56972589-56972608 | None:intergenic | 25.0% |
| ! | ATCCGTATAGTTCATTTAAA+TGG | + | chr5.1:56972584-56972603 | MS.gene67242:CDS | 25.0% |
| TCTATGATACTTTCAAGATG+AGG | - | chr5.1:56972974-56972993 | None:intergenic | 30.0% | |
| ACAGAAACATTGTGAGTCTA+AGG | + | chr5.1:56972901-56972920 | MS.gene67242:CDS | 35.0% | |
| ! | GCTTAGAACATGATTTGGAT+TGG | - | chr5.1:56972660-56972679 | None:intergenic | 35.0% |
| ! | GGTTTGCTTAGAACATGATT+TGG | - | chr5.1:56972665-56972684 | None:intergenic | 35.0% |
| !! | GAATAGCTTAAGGTGTTGTT+TGG | + | chr5.1:56972529-56972548 | MS.gene67242:CDS | 35.0% |
| ATTACACGTCCAATTTCAGC+TGG | + | chr5.1:56972620-56972639 | MS.gene67242:CDS | 40.0% | |
| TTACACGTCCAATTTCAGCT+GGG | + | chr5.1:56972621-56972640 | MS.gene67242:CDS | 40.0% | |
| AGAACTTCCCCAGCTGAAAT+TGG | - | chr5.1:56972632-56972651 | None:intergenic | 45.0% | |
| ATCAAGTCTAGTGCTCCACA+TGG | - | chr5.1:56972566-56972585 | None:intergenic | 45.0% | |
| CGTCGTTACCGGAGAAAAAA+CGG | + | chr5.1:56972774-56972793 | MS.gene67242:CDS | 45.0% | |
| CTAAGGAGATGAAAAGCTCG+AGG | + | chr5.1:56972918-56972937 | MS.gene67242:CDS | 45.0% | |
| CTTCTTGCTCTTCTCTTCAG+AGG | - | chr5.1:56972855-56972874 | None:intergenic | 45.0% | |
| GTTACCGGAGAAAAAACGGA+GGG | + | chr5.1:56972778-56972797 | MS.gene67242:CDS | 45.0% | |
| TACACGTCCAATTTCAGCTG+GGG | + | chr5.1:56972622-56972641 | MS.gene67242:CDS | 45.0% | |
| ! | CTAAGCAAACCAAGCTCTGA+AGG | + | chr5.1:56972674-56972693 | MS.gene67242:CDS | 45.0% |
| !! | TTGGCTTGTGTTCTTCCATG+TGG | + | chr5.1:56972548-56972567 | MS.gene67242:CDS | 45.0% |
| !!! | TTTTTTCTCCGGTAACGACG+TGG | - | chr5.1:56972774-56972793 | None:intergenic | 45.0% |
| CAAGATGAGGTTGCCAGCTA+CGG | - | chr5.1:56972961-56972980 | None:intergenic | 50.0% | |
| CGTTACCGGAGAAAAAACGG+AGG | + | chr5.1:56972777-56972796 | MS.gene67242:CDS | 50.0% | |
| !!! | CTGACCCTCCGTTTTTTCTC+CGG | - | chr5.1:56972785-56972804 | None:intergenic | 50.0% |
| CCGGATTCTCATCCTTTCGC+CGG | + | chr5.1:56972706-56972725 | MS.gene67242:CDS | 55.0% | |
| CCGGCGAAAGGATGAGAATC+CGG | - | chr5.1:56972709-56972728 | None:intergenic | 55.0% | |
| CCTCTCTTGAGCTCAGTCTC+CGG | - | chr5.1:56972728-56972747 | None:intergenic | 55.0% | |
| CGAACGACTCCTTCAGAGCT+TGG | - | chr5.1:56972686-56972705 | None:intergenic | 55.0% | |
| GATCCCATCCACGTCGTTAC+CGG | + | chr5.1:56972763-56972782 | MS.gene67242:CDS | 55.0% | |
| TCTCCGGTAACGACGTGGAT+GGG | - | chr5.1:56972769-56972788 | None:intergenic | 55.0% | |
| TTCTCCGGTAACGACGTGGA+TGG | - | chr5.1:56972770-56972789 | None:intergenic | 55.0% | |
| AAAGCTCGAGGCGAGATCGC+CGG | + | chr5.1:56972930-56972949 | MS.gene67242:CDS | 60.0% | |
| CCGGAGACTGAGCTCAAGAG+AGG | + | chr5.1:56972725-56972744 | MS.gene67242:CDS | 60.0% | |
| GTTGCCAGCTACGGCTATGC+CGG | - | chr5.1:56972952-56972971 | None:intergenic | 60.0% | |
| TGAGCTCAGTCTCCGGCGAA+AGG | - | chr5.1:56972721-56972740 | None:intergenic | 60.0% | |
| !! | GAGGGTCAGAAGAAGCGTCG+CGG | + | chr5.1:56972796-56972815 | MS.gene67242:CDS | 60.0% |
| !! | GCTCTGAAGGAGTCGTTCGC+CGG | + | chr5.1:56972687-56972706 | MS.gene67242:CDS | 60.0% |
| ATCGCCGGCATAGCCGTAGC+TGG | + | chr5.1:56972945-56972964 | MS.gene67242:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 56972524 | 56973006 | 56972524 | ID=MS.gene67242 |
| chr5.1 | mRNA | 56972524 | 56973006 | 56972524 | ID=MS.gene67242.t1;Parent=MS.gene67242 |
| chr5.1 | exon | 56972524 | 56973006 | 56972524 | ID=MS.gene67242.t1.exon1;Parent=MS.gene67242.t1 |
| chr5.1 | CDS | 56972524 | 56973006 | 56972524 | ID=cds.MS.gene67242.t1;Parent=MS.gene67242.t1 |
| Gene Sequence |
| Protein sequence |
