Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68852.t1 | XP_003610465.2 | 97.9 | 233 | 5 | 0 | 1 | 233 | 102 | 334 | 6.70E-137 | 496.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68852.t1 | A0A0C3X8T8 | 97.9 | 233 | 5 | 0 | 1 | 233 | 102 | 334 | 4.8e-137 | 496.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049418 | MS.gene68852 | 0.824569 | 7.52E-54 | -1.69E-46 |
| MS.gene050310 | MS.gene68852 | 0.801353 | 9.37E-49 | -1.69E-46 |
| MS.gene051018 | MS.gene68852 | 0.830487 | 2.86E-55 | -1.69E-46 |
| MS.gene051478 | MS.gene68852 | 0.807212 | 5.65E-50 | -1.69E-46 |
| MS.gene051720 | MS.gene68852 | 0.811613 | 6.41E-51 | -1.69E-46 |
| MS.gene053144 | MS.gene68852 | 0.827034 | 1.95E-54 | -1.69E-46 |
| MS.gene053538 | MS.gene68852 | 0.801471 | 8.87E-49 | -1.69E-46 |
| MS.gene054584 | MS.gene68852 | 0.829477 | 5.04E-55 | -1.69E-46 |
| MS.gene055466 | MS.gene68852 | 0.800305 | 1.53E-48 | -1.69E-46 |
| MS.gene055537 | MS.gene68852 | 0.821035 | 5.00E-53 | -1.69E-46 |
| MS.gene056169 | MS.gene68852 | 0.801934 | 7.12E-49 | -1.69E-46 |
| MS.gene058909 | MS.gene68852 | 0.803101 | 4.09E-49 | -1.69E-46 |
| MS.gene060519 | MS.gene68852 | 0.815801 | 7.67E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68852.t1 | MTR_4g132450 | 97.854 | 233 | 5 | 0 | 1 | 233 | 102 | 334 | 7.54e-175 | 484 |
| MS.gene68852.t1 | MTR_2g023970 | 78.112 | 233 | 50 | 1 | 1 | 233 | 89 | 320 | 7.71e-133 | 377 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68852.t1 | AT1G11915 | 64.831 | 236 | 78 | 5 | 1 | 233 | 96 | 329 | 4.30e-95 | 281 |
Find 39 sgRNAs with CRISPR-Local
Find 69 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTTACTGCTATTTCAGCTTT+TGG | 0.228291 | 8.4:-1575883 | MS.gene68852:CDS |
| TTGGAATTGGAGGTGGTATT+TGG | 0.228294 | 8.4:-1575855 | MS.gene68852:CDS |
| CAAGTTATGCTAGTGTTTAT+TGG | 0.239276 | 8.4:-1576593 | MS.gene68852:CDS |
| TATGATCAAGTTCCTCAATA+TGG | 0.240179 | 8.4:-1576546 | MS.gene68852:CDS |
| CTTTGAACTCCACATGTTAC+TGG | 0.265726 | 8.4:+1575805 | None:intergenic |
| CTCAAAGATTCAGTAGCTTT+AGG | 0.294315 | 8.4:+1576636 | None:intergenic |
| ACAAGTTCCACCACCACCTT+TGG | 0.367403 | 8.4:+1576464 | None:intergenic |
| TGTTCACAAAACACAGGCTT+TGG | 0.373458 | 8.4:+1576765 | None:intergenic |
| CAAGATGAATCACAGTGTTC+AGG | 0.400798 | 8.4:+1576738 | None:intergenic |
| GTGCTTGTGTCTAAACTAAA+TGG | 0.403773 | 8.4:+1576858 | None:intergenic |
| AGGAATGTCCATGTAATTGC+AGG | 0.403998 | 8.4:-1576035 | MS.gene68852:intron |
| CTAAAGCTACTGAATCTTTG+AGG | 0.411355 | 8.4:-1576635 | MS.gene68852:CDS |
| ATGATCAAGTTCCTCAATAT+GGG | 0.412810 | 8.4:-1576545 | MS.gene68852:CDS |
| TTCAATCACCTGCAATTACA+TGG | 0.419145 | 8.4:+1576027 | None:intergenic |
| TGAAGACACCGCGTCGTCAC+TGG | 0.426939 | 8.4:+1576501 | None:intergenic |
| GAGTTTCTTGTGTTTATGTA+AGG | 0.427897 | 8.4:-1576421 | MS.gene68852:CDS |
| TATTGAGGAACTTGATCATA+AGG | 0.446946 | 8.4:+1576549 | None:intergenic |
| TTTGGAGCACTTGGAATTGG+AGG | 0.470180 | 8.4:-1575865 | MS.gene68852:CDS |
| AATTTCACTACTCATTGTAA+TGG | 0.476094 | 8.4:-1576393 | MS.gene68852:intron |
| TCTAAACTAAATGGTCTTGT+TGG | 0.485018 | 8.4:+1576867 | None:intergenic |
| GACGCGGTGTCTTCAATGTC+AGG | 0.485220 | 8.4:-1576493 | MS.gene68852:CDS |
| GAGTTCAAAGCAATGAGAAC+AGG | 0.485646 | 8.4:-1575792 | MS.gene68852:CDS |
| AGTGTTTATTGGAGAAATGT+TGG | 0.491654 | 8.4:-1576582 | MS.gene68852:CDS |
| TTCTTGTGTTTATGTAAGGA+AGG | 0.498390 | 8.4:-1576417 | MS.gene68852:CDS |
| GATCAATCCAAAGGTGGTGG+TGG | 0.501256 | 8.4:-1576471 | MS.gene68852:CDS |
| ATGATAATGCAAGGCACAGT+AGG | 0.505376 | 8.4:-1576055 | MS.gene68852:CDS |
| GGAGCACTTGGAATTGGAGG+TGG | 0.523403 | 8.4:-1575862 | MS.gene68852:CDS |
| AAAGGTGGTGGTGGAACTTG+TGG | 0.581771 | 8.4:-1576462 | MS.gene68852:CDS |
| CAGGATCAATCCAAAGGTGG+TGG | 0.583668 | 8.4:-1576474 | MS.gene68852:CDS |
| TCAACTCTAATCCCATATTG+AGG | 0.585217 | 8.4:+1576534 | None:intergenic |
| GGAAATTGTTCACAAAACAC+AGG | 0.597733 | 8.4:+1576759 | None:intergenic |
| TTGATATTCCAGTGACGACG+CGG | 0.620201 | 8.4:-1576509 | MS.gene68852:CDS |
| TGGCAGATCATGATAATGCA+AGG | 0.621886 | 8.4:-1576064 | MS.gene68852:intron |
| GGAACTTGATCATAAGGCTG+AGG | 0.624162 | 8.4:+1576555 | None:intergenic |
| GCTAAAGAACCAGTAACATG+TGG | 0.630947 | 8.4:-1575814 | MS.gene68852:CDS |
| CTCATAGCAAAAGAACACTC+TGG | 0.643337 | 8.4:+1576684 | None:intergenic |
| CAATGTCAGGATCAATCCAA+AGG | 0.643953 | 8.4:-1576480 | MS.gene68852:CDS |
| TTCATGTGGAACTGTGAAGA+CGG | 0.666043 | 8.4:-1576903 | MS.gene68852:CDS |
| TGTCAGGATCAATCCAAAGG+TGG | 0.681857 | 8.4:-1576477 | MS.gene68852:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TGATTTTGAATTAATTTAAT+TGG | - | chr8.4:1576582-1576601 | MS.gene68852:CDS | 10.0% |
| !! | AATTGGATTTATTATTTGTT+TGG | - | chr8.4:1576599-1576618 | MS.gene68852:CDS | 15.0% |
| !!! | TACTTTTTTAATCATGTTAT+TGG | - | chr8.4:1576378-1576397 | MS.gene68852:intron | 15.0% |
| !!! | AACTATTGAGTAACAATTTT+TGG | - | chr8.4:1576558-1576577 | MS.gene68852:CDS | 20.0% |
| ! | AATTAACATTCACAAAGTCA+GGG | - | chr8.4:1576702-1576721 | MS.gene68852:CDS | 25.0% |
| ! | AATTTCACTACTCATTGTAA+TGG | - | chr8.4:1576290-1576309 | MS.gene68852:intron | 25.0% |
| ! | TAATTAACATTCACAAAGTC+AGG | - | chr8.4:1576701-1576720 | MS.gene68852:CDS | 25.0% |
| ! | TCACAATCACAAGATAAAAT+AGG | + | chr8.4:1576337-1576356 | None:intergenic | 25.0% |
| !!! | GGTTTTTGAAGTTGAAAATT+AGG | - | chr8.4:1576723-1576742 | MS.gene68852:CDS | 25.0% |
| AGTGTTTATTGGAGAAATGT+TGG | - | chr8.4:1576101-1576120 | MS.gene68852:intron | 30.0% | |
| ATGATCAAGTTCCTCAATAT+GGG | - | chr8.4:1576138-1576157 | MS.gene68852:intron | 30.0% | |
| CAAGTTATGCTAGTGTTTAT+TGG | - | chr8.4:1576090-1576109 | MS.gene68852:intron | 30.0% | |
| GAAAATTAGGCTTATGTTTG+TGG | - | chr8.4:1576736-1576755 | MS.gene68852:CDS | 30.0% | |
| GAGTTTCTTGTGTTTATGTA+AGG | - | chr8.4:1576262-1576281 | MS.gene68852:intron | 30.0% | |
| TATGATCAAGTTCCTCAATA+TGG | - | chr8.4:1576137-1576156 | MS.gene68852:intron | 30.0% | |
| TATTGAGGAACTTGATCATA+AGG | + | chr8.4:1576137-1576156 | None:intergenic | 30.0% | |
| TTATCTTGTGATTGTGACTT+AGG | - | chr8.4:1576340-1576359 | MS.gene68852:intron | 30.0% | |
| TTCTTGTGTTTATGTAAGGA+AGG | - | chr8.4:1576266-1576285 | MS.gene68852:intron | 30.0% | |
| !! | TCTAAACTAAATGGTCTTGT+TGG | + | chr8.4:1575819-1575838 | None:intergenic | 30.0% |
| ACTTGGATGATTACCTTTAG+TGG | - | chr8.4:1576417-1576436 | MS.gene68852:CDS | 35.0% | |
| CACTCGTACTAACATAGTAT+TGG | - | chr8.4:1576518-1576537 | MS.gene68852:CDS | 35.0% | |
| CTAAAGCTACTGAATCTTTG+AGG | - | chr8.4:1576048-1576067 | MS.gene68852:CDS | 35.0% | |
| CTCAAAGATTCAGTAGCTTT+AGG | + | chr8.4:1576050-1576069 | None:intergenic | 35.0% | |
| CTTGGATGATTACCTTTAGT+GGG | - | chr8.4:1576418-1576437 | MS.gene68852:CDS | 35.0% | |
| GGAAATTGTTCACAAAACAC+AGG | + | chr8.4:1575927-1575946 | None:intergenic | 35.0% | |
| GTGCTTGTGTCTAAACTAAA+TGG | + | chr8.4:1575828-1575847 | None:intergenic | 35.0% | |
| TATGTTAGTACGAGTGAAAC+TGG | + | chr8.4:1576514-1576533 | None:intergenic | 35.0% | |
| TCAACTCTAATCCCATATTG+AGG | + | chr8.4:1576152-1576171 | None:intergenic | 35.0% | |
| TTCAATCACCTGCAATTACA+TGG | + | chr8.4:1576659-1576678 | None:intergenic | 35.0% | |
| TTGGATGATTACCTTTAGTG+GGG | - | chr8.4:1576419-1576438 | MS.gene68852:CDS | 35.0% | |
| ! | GTTTTGCTTTGTGTTGGATT+TGG | - | chr8.4:1576758-1576777 | MS.gene68852:CDS | 35.0% |
| ! | TTGTGTTGGATTTGGAATTC+TGG | - | chr8.4:1576766-1576785 | MS.gene68852:CDS | 35.0% |
| !! | CGTACTAACATAGTATTGGT+AGG | - | chr8.4:1576522-1576541 | MS.gene68852:CDS | 35.0% |
| !! | TTTGTGGTTTTGCTTTGTGT+TGG | - | chr8.4:1576752-1576771 | MS.gene68852:CDS | 35.0% |
| !!! | GTTACTGCTATTTCAGCTTT+TGG | - | chr8.4:1576800-1576819 | MS.gene68852:CDS | 35.0% |
| AAGTTCCAAAACCCCACTAA+AGG | + | chr8.4:1576433-1576452 | None:intergenic | 40.0% | |
| AGGAATGTCCATGTAATTGC+AGG | - | chr8.4:1576648-1576667 | MS.gene68852:CDS | 40.0% | |
| CAAGATGAATCACAGTGTTC+AGG | + | chr8.4:1575948-1575967 | None:intergenic | 40.0% | |
| CAATGTCAGGATCAATCCAA+AGG | - | chr8.4:1576203-1576222 | MS.gene68852:intron | 40.0% | |
| CTCATAGCAAAAGAACACTC+TGG | + | chr8.4:1576002-1576021 | None:intergenic | 40.0% | |
| CTTTGAACTCCACATGTTAC+TGG | + | chr8.4:1576881-1576900 | None:intergenic | 40.0% | |
| GAACCTCAGATCTTGCTTAA+GGG | - | chr8.4:1576480-1576499 | MS.gene68852:CDS | 40.0% | |
| GAGTTCAAAGCAATGAGAAC+AGG | - | chr8.4:1576891-1576910 | MS.gene68852:CDS | 40.0% | |
| GCTAAAGAACCAGTAACATG+TGG | - | chr8.4:1576869-1576888 | MS.gene68852:CDS | 40.0% | |
| TGTTCACAAAACACAGGCTT+TGG | + | chr8.4:1575921-1575940 | None:intergenic | 40.0% | |
| TTGGAATTGGAGGTGGTATT+TGG | - | chr8.4:1576828-1576847 | MS.gene68852:CDS | 40.0% | |
| ! | ATGATAATGCAAGGCACAGT+AGG | - | chr8.4:1576628-1576647 | MS.gene68852:CDS | 40.0% |
| ! | GATTACCTTTAGTGGGGTTT+TGG | - | chr8.4:1576425-1576444 | MS.gene68852:CDS | 40.0% |
| ! | GTGCAAAGTATCTATCGACT+TGG | - | chr8.4:1576400-1576419 | MS.gene68852:CDS | 40.0% |
| ! | TGGCAGATCATGATAATGCA+AGG | - | chr8.4:1576619-1576638 | MS.gene68852:CDS | 40.0% |
| !! | GAGTGTTCTTTTGCTATGAG+TGG | - | chr8.4:1576002-1576021 | MS.gene68852:intron | 40.0% |
| !!! | ATTTCAGCTTTTGGAGCACT+TGG | - | chr8.4:1576809-1576828 | MS.gene68852:CDS | 40.0% |
| CGAACCTCAGATCTTGCTTA+AGG | - | chr8.4:1576479-1576498 | MS.gene68852:CDS | 45.0% | |
| GATCCCTTAAGCAAGATCTG+AGG | + | chr8.4:1576486-1576505 | None:intergenic | 45.0% | |
| GGAACTTGATCATAAGGCTG+AGG | + | chr8.4:1576131-1576150 | None:intergenic | 45.0% | |
| TGAGGTTCGAACTTTGTCGA+TGG | + | chr8.4:1576468-1576487 | None:intergenic | 45.0% | |
| TGTCAGGATCAATCCAAAGG+TGG | - | chr8.4:1576206-1576225 | MS.gene68852:intron | 45.0% | |
| TTGATATTCCAGTGACGACG+CGG | - | chr8.4:1576174-1576193 | MS.gene68852:intron | 45.0% | |
| ! | GATTTGGAATTCTGGCTGCA+GGG | - | chr8.4:1576774-1576793 | MS.gene68852:CDS | 45.0% |
| ! | TTTGGAGCACTTGGAATTGG+AGG | - | chr8.4:1576818-1576837 | MS.gene68852:CDS | 45.0% |
| !!! | GCTTTTGGAGCACTTGGAAT+TGG | - | chr8.4:1576815-1576834 | MS.gene68852:CDS | 45.0% |
| ACAAGTTCCACCACCACCTT+TGG | + | chr8.4:1576222-1576241 | None:intergenic | 50.0% | |
| CAGGATCAATCCAAAGGTGG+TGG | - | chr8.4:1576209-1576228 | MS.gene68852:intron | 50.0% | |
| ! | GATCAATCCAAAGGTGGTGG+TGG | - | chr8.4:1576212-1576231 | MS.gene68852:intron | 50.0% |
| ! | GGATTTGGAATTCTGGCTGC+AGG | - | chr8.4:1576773-1576792 | MS.gene68852:CDS | 50.0% |
| !! | AAAGGTGGTGGTGGAACTTG+TGG | - | chr8.4:1576221-1576240 | MS.gene68852:intron | 50.0% |
| GACGCGGTGTCTTCAATGTC+AGG | - | chr8.4:1576190-1576209 | MS.gene68852:intron | 55.0% | |
| ! | GGAGCACTTGGAATTGGAGG+TGG | - | chr8.4:1576821-1576840 | MS.gene68852:CDS | 55.0% |
| TGAAGACACCGCGTCGTCAC+TGG | + | chr8.4:1576185-1576204 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 1575783 | 1576922 | 1575783 | ID=MS.gene68852 |
| chr8.4 | mRNA | 1575783 | 1576922 | 1575783 | ID=MS.gene68852.t1;Parent=MS.gene68852 |
| chr8.4 | exon | 1576394 | 1576922 | 1576394 | ID=MS.gene68852.t1.exon1;Parent=MS.gene68852.t1 |
| chr8.4 | CDS | 1576394 | 1576922 | 1576394 | ID=cds.MS.gene68852.t1;Parent=MS.gene68852.t1 |
| chr8.4 | exon | 1576036 | 1576080 | 1576036 | ID=MS.gene68852.t1.exon2;Parent=MS.gene68852.t1 |
| chr8.4 | CDS | 1576036 | 1576080 | 1576036 | ID=cds.MS.gene68852.t1;Parent=MS.gene68852.t1 |
| chr8.4 | exon | 1575783 | 1575910 | 1575783 | ID=MS.gene68852.t1.exon3;Parent=MS.gene68852.t1 |
| chr8.4 | CDS | 1575783 | 1575910 | 1575783 | ID=cds.MS.gene68852.t1;Parent=MS.gene68852.t1 |
| Gene Sequence |
| Protein sequence |
