Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70798.t1 | XP_003591035.2 | 94.5 | 273 | 15 | 0 | 4 | 276 | 51 | 323 | 5.50E-138 | 500.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70798.t1 | Q43317 | 75.1 | 273 | 68 | 0 | 4 | 276 | 52 | 324 | 9.6e-115 | 414.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70798.t1 | G7I7F0 | 94.5 | 273 | 15 | 0 | 4 | 276 | 51 | 323 | 4.0e-138 | 500.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04933 | MS.gene70798 | 0.800969 | 1.12E-48 | -1.69E-46 |
| MS.gene050820 | MS.gene70798 | 0.817174 | 3.78E-52 | -1.69E-46 |
| MS.gene052011 | MS.gene70798 | -0.829824 | 4.15E-55 | -1.69E-46 |
| MS.gene054183 | MS.gene70798 | 0.823378 | 1.43E-53 | -1.69E-46 |
| MS.gene054473 | MS.gene70798 | 0.809284 | 2.04E-50 | -1.69E-46 |
| MS.gene054920 | MS.gene70798 | 0.830943 | 2.21E-55 | -1.69E-46 |
| MS.gene05594 | MS.gene70798 | 0.815922 | 7.21E-52 | -1.69E-46 |
| MS.gene056723 | MS.gene70798 | 0.811322 | 7.42E-51 | -1.69E-46 |
| MS.gene057121 | MS.gene70798 | 0.819497 | 1.13E-52 | -1.69E-46 |
| MS.gene057476 | MS.gene70798 | 0.81678 | 4.63E-52 | -1.69E-46 |
| MS.gene058736 | MS.gene70798 | 0.846658 | 1.89E-59 | -1.69E-46 |
| MS.gene058738 | MS.gene70798 | 0.801167 | 1.02E-48 | -1.69E-46 |
| MS.gene058739 | MS.gene70798 | 0.908715 | 1.26E-81 | -1.69E-46 |
| MS.gene059420 | MS.gene70798 | 0.813206 | 2.88E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70798.t1 | MTR_1g081620 | 94.505 | 273 | 15 | 0 | 4 | 276 | 51 | 323 | 2.04e-177 | 491 |
| MS.gene70798.t1 | MTR_1g081625 | 88.278 | 273 | 32 | 0 | 4 | 276 | 51 | 323 | 2.08e-162 | 454 |
| MS.gene70798.t1 | MTR_1g081610 | 76.557 | 273 | 64 | 0 | 4 | 276 | 52 | 324 | 5.38e-133 | 379 |
| MS.gene70798.t1 | MTR_5g006340 | 75.458 | 273 | 67 | 0 | 4 | 276 | 52 | 324 | 8.56e-132 | 376 |
| MS.gene70798.t1 | MTR_1g081640 | 84.388 | 237 | 37 | 0 | 4 | 240 | 51 | 287 | 2.15e-131 | 375 |
| MS.gene70798.t1 | MTR_1g103690 | 74.359 | 273 | 70 | 0 | 4 | 276 | 52 | 324 | 1.16e-129 | 371 |
| MS.gene70798.t1 | MTR_4g087490 | 64.444 | 270 | 96 | 0 | 4 | 273 | 91 | 360 | 4.37e-129 | 371 |
| MS.gene70798.t1 | MTR_4g087510 | 61.481 | 270 | 96 | 1 | 4 | 273 | 90 | 351 | 6.74e-121 | 350 |
| MS.gene70798.t1 | MTR_7g078070 | 62.825 | 269 | 100 | 0 | 5 | 273 | 13 | 281 | 1.89e-119 | 343 |
| MS.gene70798.t1 | MTR_7g078070 | 62.825 | 269 | 100 | 0 | 5 | 273 | 46 | 314 | 5.11e-119 | 343 |
| MS.gene70798.t1 | MTR_7g078070 | 62.825 | 269 | 100 | 0 | 5 | 273 | 106 | 374 | 9.04e-119 | 345 |
| MS.gene70798.t1 | MTR_4g087520 | 66.917 | 266 | 88 | 0 | 8 | 273 | 1 | 266 | 3.97e-116 | 334 |
| MS.gene70798.t1 | MTR_7g087110 | 59.259 | 270 | 100 | 1 | 4 | 273 | 109 | 368 | 5.85e-99 | 294 |
| MS.gene70798.t1 | MTR_5g095670 | 29.073 | 313 | 163 | 5 | 4 | 259 | 88 | 398 | 4.49e-34 | 128 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70798.t1 | AT4G14880 | 70.330 | 273 | 81 | 0 | 4 | 276 | 49 | 321 | 8.51e-128 | 366 |
| MS.gene70798.t1 | AT4G14880 | 70.330 | 273 | 81 | 0 | 4 | 276 | 49 | 321 | 8.51e-128 | 366 |
| MS.gene70798.t1 | AT4G14880 | 70.330 | 273 | 81 | 0 | 4 | 276 | 49 | 321 | 8.51e-128 | 366 |
| MS.gene70798.t1 | AT4G14880 | 70.330 | 273 | 81 | 0 | 4 | 276 | 49 | 321 | 8.51e-128 | 366 |
| MS.gene70798.t1 | AT4G14880 | 70.330 | 273 | 81 | 0 | 4 | 276 | 49 | 321 | 8.51e-128 | 366 |
| MS.gene70798.t1 | AT2G43750 | 72.593 | 270 | 74 | 0 | 4 | 273 | 119 | 388 | 1.57e-127 | 368 |
| MS.gene70798.t1 | AT2G43750 | 72.593 | 270 | 74 | 0 | 4 | 273 | 119 | 388 | 1.57e-127 | 368 |
| MS.gene70798.t1 | AT3G59760 | 71.111 | 270 | 78 | 0 | 4 | 273 | 157 | 426 | 1.03e-126 | 367 |
| MS.gene70798.t1 | AT3G59760 | 71.111 | 270 | 78 | 0 | 4 | 273 | 157 | 426 | 1.08e-126 | 367 |
| MS.gene70798.t1 | AT3G04940 | 70.000 | 270 | 81 | 0 | 4 | 273 | 52 | 321 | 3.75e-126 | 362 |
| MS.gene70798.t1 | AT3G04940 | 70.000 | 270 | 81 | 0 | 4 | 273 | 52 | 321 | 3.75e-126 | 362 |
| MS.gene70798.t1 | AT3G22460 | 67.658 | 269 | 67 | 1 | 8 | 276 | 1 | 249 | 2.04e-123 | 352 |
| MS.gene70798.t1 | AT5G28020 | 67.399 | 273 | 89 | 0 | 4 | 276 | 51 | 323 | 3.46e-116 | 337 |
| MS.gene70798.t1 | AT5G28020 | 67.399 | 273 | 89 | 0 | 4 | 276 | 51 | 323 | 3.46e-116 | 337 |
| MS.gene70798.t1 | AT5G28020 | 67.399 | 273 | 89 | 0 | 4 | 276 | 51 | 323 | 3.46e-116 | 337 |
| MS.gene70798.t1 | AT5G28020 | 67.399 | 273 | 89 | 0 | 4 | 276 | 51 | 323 | 3.46e-116 | 337 |
| MS.gene70798.t1 | AT5G28020 | 67.399 | 273 | 89 | 0 | 4 | 276 | 51 | 323 | 3.46e-116 | 337 |
| MS.gene70798.t1 | AT5G28030 | 68.132 | 273 | 87 | 0 | 4 | 276 | 51 | 323 | 6.59e-115 | 333 |
| MS.gene70798.t1 | AT5G28030 | 68.132 | 273 | 87 | 0 | 4 | 276 | 51 | 323 | 6.59e-115 | 333 |
| MS.gene70798.t1 | AT5G28030 | 68.132 | 273 | 87 | 0 | 4 | 276 | 51 | 323 | 6.59e-115 | 333 |
| MS.gene70798.t1 | AT5G28030 | 68.132 | 273 | 87 | 0 | 4 | 276 | 51 | 323 | 6.59e-115 | 333 |
| MS.gene70798.t1 | AT3G61440 | 62.030 | 266 | 101 | 0 | 8 | 273 | 1 | 266 | 4.07e-114 | 329 |
| MS.gene70798.t1 | AT3G61440 | 61.338 | 269 | 104 | 0 | 5 | 273 | 94 | 362 | 6.71e-114 | 332 |
| MS.gene70798.t1 | AT3G59760 | 66.920 | 263 | 86 | 1 | 4 | 266 | 157 | 418 | 2.84e-109 | 323 |
| MS.gene70798.t1 | AT3G03630 | 61.029 | 272 | 96 | 1 | 4 | 275 | 143 | 404 | 3.43e-100 | 299 |
| MS.gene70798.t1 | AT3G61440 | 60.833 | 240 | 94 | 0 | 34 | 273 | 2 | 241 | 3.78e-100 | 293 |
| MS.gene70798.t1 | AT5G28020 | 66.245 | 237 | 80 | 0 | 40 | 276 | 1 | 237 | 3.58e-95 | 280 |
| MS.gene70798.t1 | AT3G61440 | 59.361 | 219 | 89 | 0 | 5 | 223 | 94 | 312 | 7.64e-89 | 267 |
| MS.gene70798.t1 | AT5G28030 | 66.995 | 203 | 67 | 0 | 4 | 206 | 51 | 253 | 3.65e-84 | 253 |
| MS.gene70798.t1 | AT3G22460 | 72.993 | 137 | 37 | 0 | 4 | 140 | 52 | 188 | 4.57e-69 | 212 |
| MS.gene70798.t1 | AT1G55880 | 27.707 | 314 | 167 | 7 | 4 | 259 | 84 | 395 | 1.17e-29 | 116 |
| MS.gene70798.t1 | AT1G55880 | 31.401 | 207 | 100 | 5 | 4 | 169 | 84 | 289 | 2.64e-24 | 100 |
Find 71 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTACTAGATGAAGTTATTC+AGG | 0.259370 | 1.2:-60985094 | MS.gene70798:intron |
| TGCCTTGAAAGAAGGTTTAC+TGG | 0.283658 | 1.2:-60984738 | MS.gene70798:intron |
| TTGGTTGCAGGGATAGGAAC+TGG | 0.301174 | 1.2:-60986119 | MS.gene70798:CDS |
| ATTTGACTAATTACCTTTCC+AGG | 0.303581 | 1.2:+60986922 | None:intergenic |
| AGAGTTGATGCTTTGGTTGC+AGG | 0.314791 | 1.2:-60986131 | MS.gene70798:CDS |
| AGACCACTGGCCCTGAGATT+TGG | 0.324065 | 1.2:-60986169 | MS.gene70798:CDS |
| TGGAGGGAGAGTTGATGCTT+TGG | 0.358657 | 1.2:-60986138 | MS.gene70798:CDS |
| ACTATTCATACCTTGATCTC+TGG | 0.360280 | 1.2:+60986053 | None:intergenic |
| CCAGAGTCTTTCCAAATCTC+AGG | 0.373679 | 1.2:+60986158 | None:intergenic |
| CATCTAGTAGATCAACTTCC+AGG | 0.383555 | 1.2:+60985106 | None:intergenic |
| AAAATTATACTGCGAGCTTT+CGG | 0.393798 | 1.2:-60986411 | MS.gene70798:CDS |
| TGATTGAGCATATAACTATT+AGG | 0.407097 | 1.2:+60986309 | None:intergenic |
| TACCAGTAAACCTTCTTTCA+AGG | 0.409533 | 1.2:+60984736 | None:intergenic |
| ATGCCGGTGTTACCACTTGT+TGG | 0.412993 | 1.2:+60986510 | None:intergenic |
| TTGCAGATGGGAATTTCATC+AGG | 0.417954 | 1.2:-60945549 | MS.gene70798:intron |
| TTCTTCAGCTTCACGCCTGA+TGG | 0.420313 | 1.2:+60945254 | None:intergenic |
| CAGCACCGATCCCTTGGATT+AGG | 0.432171 | 1.2:+60985145 | None:intergenic |
| CTATTCATACCTTGATCTCT+GGG | 0.435238 | 1.2:+60986054 | None:intergenic |
| CTTTCAAGACTCACATAAGC+TGG | 0.442324 | 1.2:+60986435 | None:intergenic |
| CGACACCAGCACCGATCCCT+TGG | 0.442965 | 1.2:+60985139 | None:intergenic |
| GACCCTGCCAAAGGAATTGA+TGG | 0.448526 | 1.2:-60986369 | MS.gene70798:CDS |
| GAGTTGATGCTTTGGTTGCA+GGG | 0.455243 | 1.2:-60986130 | MS.gene70798:CDS |
| ACACCATCAATTCCTTTGGC+AGG | 0.457831 | 1.2:+60986366 | None:intergenic |
| GTAGATCAACTTCCAGGATA+GGG | 0.464520 | 1.2:+60985112 | None:intergenic |
| TGTAAATAACACCTTTGGAT+TGG | 0.472231 | 1.2:+60986272 | None:intergenic |
| AAGCTGCTTGCCTTGAAAGA+AGG | 0.474497 | 1.2:-60984746 | MS.gene70798:CDS |
| TGGTTGCAGGGATAGGAACT+GGG | 0.484341 | 1.2:-60986118 | MS.gene70798:CDS |
| GGGGAACAGTAACAGGTGTT+GGG | 0.494810 | 1.2:-60986097 | MS.gene70798:CDS |
| GATGCTTTGGTTGCAGGGAT+AGG | 0.501960 | 1.2:-60986125 | MS.gene70798:CDS |
| AGTAGATCAACTTCCAGGAT+AGG | 0.502056 | 1.2:+60985111 | None:intergenic |
| GCAGCAGCTGCAATAAAACT+AGG | 0.508019 | 1.2:-60945519 | MS.gene70798:CDS |
| TCTGAAAGTGCAGTTCTGAA+TGG | 0.514085 | 1.2:-60985948 | MS.gene70798:CDS |
| ATTGAAGATGCCGAGAGTAA+AGG | 0.514802 | 1.2:-60986955 | MS.gene70798:CDS |
| AAGTAACGCTCTCCAAAACT+AGG | 0.515786 | 1.2:+60945297 | None:intergenic |
| CTAATCCAAGGGATCGGTGC+TGG | 0.516701 | 1.2:-60985144 | MS.gene70798:CDS |
| TTACCTTTCCAGGTGTAATG+AGG | 0.523442 | 1.2:+60986932 | None:intergenic |
| TTCAGAACTGCACTTTCAGA+TGG | 0.538348 | 1.2:+60985951 | None:intergenic |
| CCTGAGATTTGGAAAGACTC+TGG | 0.544001 | 1.2:-60986158 | MS.gene70798:CDS |
| ACAAGTGGTAACACCGGCAT+AGG | 0.544602 | 1.2:-60986507 | MS.gene70798:CDS |
| GTTCTGAATGGAGGAAAGCC+AGG | 0.546295 | 1.2:-60985936 | MS.gene70798:intron |
| TAGATTCATTACGAGACCAC+TGG | 0.548191 | 1.2:-60986182 | MS.gene70798:CDS |
| TGTAATGAGGCCTTTACTCT+CGG | 0.551658 | 1.2:+60986945 | None:intergenic |
| CACCATCAATTCCTTTGGCA+GGG | 0.555618 | 1.2:+60986367 | None:intergenic |
| GGCGTCGATAGCAGCAATGA+GGG | 0.558283 | 1.2:-60986481 | MS.gene70798:CDS |
| TGGTAACACCGGCATAGGAT+TGG | 0.559661 | 1.2:-60986502 | MS.gene70798:CDS |
| AGGCCTCATTACACCTGGAA+AGG | 0.563587 | 1.2:-60986935 | MS.gene70798:intron |
| GGGAAGAGACAAGAAAATGC+AGG | 0.574537 | 1.2:-60945498 | MS.gene70798:CDS |
| AAAGGCAAACACCTAATCCA+AGG | 0.576831 | 1.2:-60985156 | MS.gene70798:intron |
| TAGATCAACTTCCAGGATAG+GGG | 0.577360 | 1.2:+60985113 | None:intergenic |
| CAGCAGCTGCAATAAAACTA+GGG | 0.584186 | 1.2:-60945518 | MS.gene70798:CDS |
| AGTAAAGGCCTCATTACACC+TGG | 0.589255 | 1.2:-60986940 | MS.gene70798:CDS |
| GTTCTTGTTGAGCCAACAAG+TGG | 0.593333 | 1.2:-60986522 | MS.gene70798:CDS |
| TTTCCAAATCTCAGGGCCAG+TGG | 0.596982 | 1.2:+60986166 | None:intergenic |
| TATCGACGCCAATCCTATGC+CGG | 0.597404 | 1.2:+60986494 | None:intergenic |
| AGATTTGGAAAGACTCTGGA+GGG | 0.598831 | 1.2:-60986154 | MS.gene70798:CDS |
| CAGAGTCTTTCCAAATCTCA+GGG | 0.601201 | 1.2:+60986159 | None:intergenic |
| TGAAAACTCTGCCAATCCAA+AGG | 0.611620 | 1.2:-60986283 | MS.gene70798:intron |
| GAGATTTGGAAAGACTCTGG+AGG | 0.616942 | 1.2:-60986155 | MS.gene70798:CDS |
| ACTGCGAGCTTTCGGAGCCG+AGG | 0.618497 | 1.2:-60986403 | MS.gene70798:CDS |
| TGGCGTCGATAGCAGCAATG+AGG | 0.618801 | 1.2:-60986482 | MS.gene70798:CDS |
| AAACACCTAATCCAAGGGAT+CGG | 0.628864 | 1.2:-60985150 | MS.gene70798:CDS |
| AGATCAACTTCCAGGATAGG+GGG | 0.642892 | 1.2:+60985114 | None:intergenic |
| AAGGCAAACACCTAATCCAA+GGG | 0.643306 | 1.2:-60985155 | MS.gene70798:CDS |
| AGGGTCTGTCAAATATACCT+CGG | 0.651115 | 1.2:+60986386 | None:intergenic |
| TATTTGACAGACCCTGCCAA+AGG | 0.662474 | 1.2:-60986378 | MS.gene70798:CDS |
| AGGTGTTGGGAAGTTCCTCA+AGG | 0.665059 | 1.2:-60986084 | MS.gene70798:CDS |
| GAAAGTGCAGTTCTGAATGG+AGG | 0.666727 | 1.2:-60985945 | MS.gene70798:CDS |
| GGTTGCAGGGATAGGAACTG+GGG | 0.673121 | 1.2:-60986117 | MS.gene70798:CDS |
| GAGCCAACAAGTGGTAACAC+CGG | 0.677684 | 1.2:-60986513 | MS.gene70798:CDS |
| GCGTCGATAGCAGCAATGAG+GGG | 0.705710 | 1.2:-60986480 | MS.gene70798:CDS |
| GTTGCAGGGATAGGAACTGG+GGG | 0.729118 | 1.2:-60986116 | MS.gene70798:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.2 | gene | 60945239 | 60988729 | 60945239 | ID=MS.gene70798 |
| chr1.2 | mRNA | 60945239 | 60988729 | 60945239 | ID=MS.gene70798.t1;Parent=MS.gene70798 |
| chr1.2 | exon | 60988722 | 60988729 | 60988722 | ID=MS.gene70798.t1.exon1;Parent=MS.gene70798.t1 |
| chr1.2 | CDS | 60988722 | 60988729 | 60988722 | ID=cds.MS.gene70798.t1;Parent=MS.gene70798.t1 |
| chr1.2 | exon | 60986936 | 60986993 | 60986936 | ID=MS.gene70798.t1.exon2;Parent=MS.gene70798.t1 |
| chr1.2 | CDS | 60986936 | 60986993 | 60986936 | ID=cds.MS.gene70798.t1;Parent=MS.gene70798.t1 |
| chr1.2 | exon | 60986284 | 60986547 | 60986284 | ID=MS.gene70798.t1.exon3;Parent=MS.gene70798.t1 |
| chr1.2 | CDS | 60986284 | 60986547 | 60986284 | ID=cds.MS.gene70798.t1;Parent=MS.gene70798.t1 |
| chr1.2 | exon | 60986064 | 60986201 | 60986064 | ID=MS.gene70798.t1.exon4;Parent=MS.gene70798.t1 |
| chr1.2 | CDS | 60986064 | 60986201 | 60986064 | ID=cds.MS.gene70798.t1;Parent=MS.gene70798.t1 |
| chr1.2 | exon | 60985937 | 60985988 | 60985937 | ID=MS.gene70798.t1.exon5;Parent=MS.gene70798.t1 |
| chr1.2 | CDS | 60985937 | 60985988 | 60985937 | ID=cds.MS.gene70798.t1;Parent=MS.gene70798.t1 |
| chr1.2 | exon | 60985095 | 60985174 | 60985095 | ID=MS.gene70798.t1.exon6;Parent=MS.gene70798.t1 |
| chr1.2 | CDS | 60985095 | 60985174 | 60985095 | ID=cds.MS.gene70798.t1;Parent=MS.gene70798.t1 |
| chr1.2 | exon | 60984739 | 60984798 | 60984739 | ID=MS.gene70798.t1.exon7;Parent=MS.gene70798.t1 |
| chr1.2 | CDS | 60984739 | 60984798 | 60984739 | ID=cds.MS.gene70798.t1;Parent=MS.gene70798.t1 |
| chr1.2 | exon | 60945485 | 60945565 | 60945485 | ID=MS.gene70798.t1.exon8;Parent=MS.gene70798.t1 |
| chr1.2 | CDS | 60945485 | 60945565 | 60945485 | ID=cds.MS.gene70798.t1;Parent=MS.gene70798.t1 |
| chr1.2 | exon | 60945239 | 60945328 | 60945239 | ID=MS.gene70798.t1.exon9;Parent=MS.gene70798.t1 |
| chr1.2 | CDS | 60945239 | 60945328 | 60945239 | ID=cds.MS.gene70798.t1;Parent=MS.gene70798.t1 |
| Gene Sequence |
| Protein sequence |
