Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72033.t1 | GAU14248.1 | 75.4 | 167 | 37 | 3 | 1 | 164 | 1 | 166 | 4.50E-69 | 270.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72033.t1 | A0A2K3NXU4 | 73.7 | 167 | 41 | 2 | 1 | 164 | 1 | 167 | 2.7e-68 | 267.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene055124 | MS.gene72033 | 0.801119 | 1.05E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene72033 | MS.gene064976 | PPI |
| MS.gene53620 | MS.gene72033 | PPI |
| MS.gene64289 | MS.gene72033 | PPI |
| MS.gene72033 | MS.gene94983 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72033.t1 | MTR_3g109880 | 60.335 | 179 | 55 | 5 | 1 | 169 | 1 | 173 | 5.68e-69 | 207 |
| MS.gene72033.t1 | MTR_6g088445 | 42.262 | 168 | 81 | 3 | 9 | 170 | 10 | 167 | 2.66e-38 | 129 |
| MS.gene72033.t1 | MTR_6g088475 | 43.038 | 158 | 74 | 3 | 9 | 160 | 10 | 157 | 4.41e-36 | 123 |
| MS.gene72033.t1 | MTR_1g046660 | 33.908 | 174 | 105 | 4 | 3 | 170 | 2 | 171 | 2.03e-24 | 94.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72033.t1 | AT4G32870 | 47.239 | 163 | 76 | 4 | 1 | 161 | 1 | 155 | 9.19e-49 | 155 |
| MS.gene72033.t1 | AT2G25770 | 36.242 | 149 | 81 | 4 | 1 | 144 | 1 | 140 | 7.08e-31 | 110 |
| MS.gene72033.t1 | AT2G25770 | 36.242 | 149 | 81 | 4 | 1 | 144 | 1 | 140 | 7.08e-31 | 110 |
Find 54 sgRNAs with CRISPR-Local
Find 57 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTCATTGGAAACGTTATTA+AGG | 0.203448 | 3.4:-95496438 | MS.gene72033:CDS |
| TGAAATTGTTGATAACAGTA+TGG | 0.310256 | 3.4:-95496606 | MS.gene72033:CDS |
| AGGAAAATAAGCCAACAAAA+TGG | 0.322964 | 3.4:-95496868 | MS.gene72033:CDS |
| TTCATTGGAAACGTTATTAA+GGG | 0.346776 | 3.4:-95496437 | MS.gene72033:CDS |
| CAAGATTTGAATTCTTTCAT+TGG | 0.349097 | 3.4:-95496452 | MS.gene72033:CDS |
| TATAGTATGGCAAGTGTTAG+AGG | 0.403359 | 3.4:-95496804 | MS.gene72033:CDS |
| GGAAAATAAGCCAACAAAAT+GGG | 0.403599 | 3.4:-95496867 | MS.gene72033:CDS |
| TCTTTAACCCACTCAGAAGT+TGG | 0.415580 | 3.4:+95496671 | None:intergenic |
| ATGGATTGTTATAAGCTTGA+AGG | 0.449145 | 3.4:-95496752 | MS.gene72033:CDS |
| TCATCATCAACGCCGCTGAT+TGG | 0.462447 | 3.4:+95496542 | None:intergenic |
| TCAAGCTTATAACAATCCAT+TGG | 0.469517 | 3.4:+95496755 | None:intergenic |
| GGTGTTTGTGATCCGGTGGA+AGG | 0.474182 | 3.4:-95496485 | MS.gene72033:CDS |
| ACTGTTATCAACAATTTCAT+AGG | 0.476144 | 3.4:+95496610 | None:intergenic |
| TATAAGCTTGAAGGGATTGA+AGG | 0.477132 | 3.4:-95496743 | MS.gene72033:CDS |
| CAAGCTTATAACAATCCATT+GGG | 0.479296 | 3.4:+95496756 | None:intergenic |
| GAAATTGTTGATAACAGTAT+GGG | 0.485941 | 3.4:-95496605 | MS.gene72033:CDS |
| ATGGGAGGGTGTTTGTGATC+CGG | 0.489610 | 3.4:-95496492 | MS.gene72033:CDS |
| GAACTGAGGAGGATATAGTA+TGG | 0.492546 | 3.4:-95496817 | MS.gene72033:CDS |
| AACCCACTCAGAAGTTGGAT+TGG | 0.500294 | 3.4:+95496676 | None:intergenic |
| AAAGCCACCGCTGAGGTAAG+AGG | 0.507470 | 3.4:-95496839 | MS.gene72033:CDS |
| TGGATTGTTATAAGCTTGAA+GGG | 0.510009 | 3.4:-95496751 | MS.gene72033:CDS |
| ATAGGTCAAACAACGTTGGA+TGG | 0.513283 | 3.4:+95496628 | None:intergenic |
| TAGCTAGTCAAAGTTTGTGT+GGG | 0.515225 | 3.4:-95496382 | MS.gene72033:CDS |
| GTGTAGGTTGTATGATCGAA+TGG | 0.523159 | 3.4:-95496511 | MS.gene72033:CDS |
| GTGTTTGTGATCCGGTGGAA+GGG | 0.541416 | 3.4:-95496484 | MS.gene72033:CDS |
| TGGAAACGTTATTAAGGGCA+TGG | 0.549534 | 3.4:-95496432 | MS.gene72033:CDS |
| AGCGAGATCAATAATGGGAG+AGG | 0.553005 | 3.4:-95496888 | None:intergenic |
| TTTGTGATCCGGTGGAAGGG+TGG | 0.560676 | 3.4:-95496481 | MS.gene72033:CDS |
| TGAACTGTCCACCCTTCCAC+CGG | 0.560925 | 3.4:+95496473 | None:intergenic |
| GAAGGGATTGAAGGTCAACC+TGG | 0.564797 | 3.4:-95496734 | MS.gene72033:CDS |
| TCAACGCCGCTGATTGGCAA+TGG | 0.566877 | 3.4:+95496548 | None:intergenic |
| TGTAGGTTGTATGATCGAAT+GGG | 0.567289 | 3.4:-95496510 | MS.gene72033:CDS |
| CCATTGGGAGCCACTTGTGT+AGG | 0.567437 | 3.4:+95496771 | None:intergenic |
| TTAGCTAGTCAAAGTTTGTG+TGG | 0.568989 | 3.4:-95496383 | MS.gene72033:CDS |
| TAGGTCAAACAACGTTGGAT+GGG | 0.573010 | 3.4:+95496629 | None:intergenic |
| GCACAATAACGAACGAGACC+AGG | 0.573329 | 3.4:+95496716 | None:intergenic |
| AAATTGTTGATAACAGTATG+GGG | 0.598578 | 3.4:-95496604 | MS.gene72033:CDS |
| TTTCATAGGTCAAACAACGT+TGG | 0.600620 | 3.4:+95496624 | None:intergenic |
| TGCCAATCCAACTTCTGAGT+GGG | 0.606140 | 3.4:-95496678 | MS.gene72033:CDS |
| AGTTCCTCTTACCTCAGCGG+TGG | 0.616369 | 3.4:+95496835 | None:intergenic |
| CCTACACAAGTGGCTCCCAA+TGG | 0.622824 | 3.4:-95496771 | MS.gene72033:CDS |
| AATAAGCCAACAAAATGGGA+AGG | 0.626979 | 3.4:-95496863 | MS.gene72033:CDS |
| GGAGGGTGTTTGTGATCCGG+TGG | 0.630744 | 3.4:-95496489 | MS.gene72033:CDS |
| CGCTGAGGTAAGAGGAACTG+AGG | 0.632981 | 3.4:-95496831 | MS.gene72033:CDS |
| CTCAGTTCCTCTTACCTCAG+CGG | 0.641075 | 3.4:+95496832 | None:intergenic |
| AGGTTGTATGATCGAATGGG+AGG | 0.648114 | 3.4:-95496507 | MS.gene72033:CDS |
| GATGATGATGCTAAACGTGT+AGG | 0.650720 | 3.4:-95496527 | MS.gene72033:CDS |
| ATTTCTGTAACCTACACAAG+TGG | 0.658716 | 3.4:-95496781 | MS.gene72033:CDS |
| TGAGGTAAGAGGAACTGAGG+AGG | 0.667990 | 3.4:-95496828 | MS.gene72033:CDS |
| GGTTGTATGATCGAATGGGA+GGG | 0.676731 | 3.4:-95496506 | MS.gene72033:CDS |
| GTGCCAATCCAACTTCTGAG+TGG | 0.684174 | 3.4:-95496679 | MS.gene72033:CDS |
| GTATTGCCATTGCCAATCAG+CGG | 0.692711 | 3.4:-95496554 | MS.gene72033:CDS |
| TATGGGGTTTAAGAATTACG+TGG | 0.696481 | 3.4:-95496588 | MS.gene72033:CDS |
| GGAAGGTAAAGCCACCGCTG+AGG | 0.701441 | 3.4:-95496846 | MS.gene72033:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ACTGTTATCAACAATTTCAT+AGG | + | chr3.4:95496641-95496660 | None:intergenic | 25.0% |
| ! | CAAGATTTGAATTCTTTCAT+TGG | - | chr3.4:95496796-95496815 | MS.gene72033:CDS | 25.0% |
| ! | TATTGTGCTTTCACAAAAAA+GGG | - | chr3.4:95496547-95496566 | MS.gene72033:CDS | 25.0% |
| ! | TTATTGTGCTTTCACAAAAA+AGG | - | chr3.4:95496546-95496565 | MS.gene72033:CDS | 25.0% |
| ! | TTCATTGGAAACGTTATTAA+GGG | - | chr3.4:95496811-95496830 | MS.gene72033:CDS | 25.0% |
| ! | TTTCATTGGAAACGTTATTA+AGG | - | chr3.4:95496810-95496829 | MS.gene72033:CDS | 25.0% |
| !! | ATATGTAAGCTCAATTTTGT+TGG | + | chr3.4:95496842-95496861 | None:intergenic | 25.0% |
| !!! | AAATTGTTGATAACAGTATG+GGG | - | chr3.4:95496644-95496663 | MS.gene72033:CDS | 25.0% |
| !!! | GAAATTGTTGATAACAGTAT+GGG | - | chr3.4:95496643-95496662 | MS.gene72033:CDS | 25.0% |
| !!! | TGAAATTGTTGATAACAGTA+TGG | - | chr3.4:95496642-95496661 | MS.gene72033:CDS | 25.0% |
| AGGAAAATAAGCCAACAAAA+TGG | - | chr3.4:95496380-95496399 | MS.gene72033:CDS | 30.0% | |
| CAAGCTTATAACAATCCATT+GGG | + | chr3.4:95496495-95496514 | None:intergenic | 30.0% | |
| GGAAAATAAGCCAACAAAAT+GGG | - | chr3.4:95496381-95496400 | MS.gene72033:CDS | 30.0% | |
| TCAAGCTTATAACAATCCAT+TGG | + | chr3.4:95496496-95496515 | None:intergenic | 30.0% | |
| TGGATTGTTATAAGCTTGAA+GGG | - | chr3.4:95496497-95496516 | MS.gene72033:CDS | 30.0% | |
| ! | ATGGATTGTTATAAGCTTGA+AGG | - | chr3.4:95496496-95496515 | MS.gene72033:CDS | 30.0% |
| AATAAGCCAACAAAATGGGA+AGG | - | chr3.4:95496385-95496404 | MS.gene72033:CDS | 35.0% | |
| ATTTCTGTAACCTACACAAG+TGG | - | chr3.4:95496467-95496486 | MS.gene72033:CDS | 35.0% | |
| TAGCTAGTCAAAGTTTGTGT+GGG | - | chr3.4:95496866-95496885 | MS.gene72033:CDS | 35.0% | |
| TATAAGCTTGAAGGGATTGA+AGG | - | chr3.4:95496505-95496524 | MS.gene72033:CDS | 35.0% | |
| TATAGTATGGCAAGTGTTAG+AGG | - | chr3.4:95496444-95496463 | MS.gene72033:CDS | 35.0% | |
| TATGGGGTTTAAGAATTACG+TGG | - | chr3.4:95496660-95496679 | MS.gene72033:CDS | 35.0% | |
| TGTAGGTTGTATGATCGAAT+GGG | - | chr3.4:95496738-95496757 | MS.gene72033:CDS | 35.0% | |
| TTAGCTAGTCAAAGTTTGTG+TGG | - | chr3.4:95496865-95496884 | MS.gene72033:CDS | 35.0% | |
| TTTCATAGGTCAAACAACGT+TGG | + | chr3.4:95496627-95496646 | None:intergenic | 35.0% | |
| ATAGGTCAAACAACGTTGGA+TGG | + | chr3.4:95496623-95496642 | None:intergenic | 40.0% | |
| GAACTGAGGAGGATATAGTA+TGG | - | chr3.4:95496431-95496450 | MS.gene72033:CDS | 40.0% | |
| GTGTAGGTTGTATGATCGAA+TGG | - | chr3.4:95496737-95496756 | MS.gene72033:CDS | 40.0% | |
| TAGGTCAAACAACGTTGGAT+GGG | + | chr3.4:95496622-95496641 | None:intergenic | 40.0% | |
| TCTTTAACCCACTCAGAAGT+TGG | + | chr3.4:95496580-95496599 | None:intergenic | 40.0% | |
| TGGAAACGTTATTAAGGGCA+TGG | - | chr3.4:95496816-95496835 | MS.gene72033:CDS | 40.0% | |
| ! | GATGATGATGCTAAACGTGT+AGG | - | chr3.4:95496721-95496740 | MS.gene72033:CDS | 40.0% |
| ! | GCTTTACCTTCCCATTTTGT+TGG | + | chr3.4:95496394-95496413 | None:intergenic | 40.0% |
| AACCCACTCAGAAGTTGGAT+TGG | + | chr3.4:95496575-95496594 | None:intergenic | 45.0% | |
| AGGTTGTATGATCGAATGGG+AGG | - | chr3.4:95496741-95496760 | MS.gene72033:CDS | 45.0% | |
| GGTTGTATGATCGAATGGGA+GGG | - | chr3.4:95496742-95496761 | MS.gene72033:CDS | 45.0% | |
| GTATTGCCATTGCCAATCAG+CGG | - | chr3.4:95496694-95496713 | MS.gene72033:CDS | 45.0% | |
| TGCCAATCCAACTTCTGAGT+GGG | - | chr3.4:95496570-95496589 | MS.gene72033:CDS | 45.0% | |
| CTCAGTTCCTCTTACCTCAG+CGG | + | chr3.4:95496419-95496438 | None:intergenic | 50.0% | |
| GAAGGGATTGAAGGTCAACC+TGG | - | chr3.4:95496514-95496533 | MS.gene72033:CDS | 50.0% | |
| GCACAATAACGAACGAGACC+AGG | + | chr3.4:95496535-95496554 | None:intergenic | 50.0% | |
| GTGCCAATCCAACTTCTGAG+TGG | - | chr3.4:95496569-95496588 | MS.gene72033:CDS | 50.0% | |
| GTGTTTGTGATCCGGTGGAA+GGG | - | chr3.4:95496764-95496783 | MS.gene72033:CDS | 50.0% | |
| TCATCATCAACGCCGCTGAT+TGG | + | chr3.4:95496709-95496728 | None:intergenic | 50.0% | |
| TGAGGTAAGAGGAACTGAGG+AGG | - | chr3.4:95496420-95496439 | MS.gene72033:CDS | 50.0% | |
| !! | ATGGGAGGGTGTTTGTGATC+CGG | - | chr3.4:95496756-95496775 | MS.gene72033:CDS | 50.0% |
| AAAGCCACCGCTGAGGTAAG+AGG | - | chr3.4:95496409-95496428 | MS.gene72033:CDS | 55.0% | |
| AGTTCCTCTTACCTCAGCGG+TGG | + | chr3.4:95496416-95496435 | None:intergenic | 55.0% | |
| CCATTGGGAGCCACTTGTGT+AGG | + | chr3.4:95496480-95496499 | None:intergenic | 55.0% | |
| CCTACACAAGTGGCTCCCAA+TGG | - | chr3.4:95496477-95496496 | MS.gene72033:CDS | 55.0% | |
| CGCTGAGGTAAGAGGAACTG+AGG | - | chr3.4:95496417-95496436 | MS.gene72033:CDS | 55.0% | |
| GGTGTTTGTGATCCGGTGGA+AGG | - | chr3.4:95496763-95496782 | MS.gene72033:CDS | 55.0% | |
| TGAACTGTCCACCCTTCCAC+CGG | + | chr3.4:95496778-95496797 | None:intergenic | 55.0% | |
| TTTGTGATCCGGTGGAAGGG+TGG | - | chr3.4:95496767-95496786 | MS.gene72033:CDS | 55.0% | |
| ! | TCAACGCCGCTGATTGGCAA+TGG | + | chr3.4:95496703-95496722 | None:intergenic | 55.0% |
| GGAAGGTAAAGCCACCGCTG+AGG | - | chr3.4:95496402-95496421 | MS.gene72033:CDS | 60.0% | |
| !! | GGAGGGTGTTTGTGATCCGG+TGG | - | chr3.4:95496759-95496778 | MS.gene72033:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 95496373 | 95496897 | 95496373 | ID=MS.gene72033 |
| chr3.4 | mRNA | 95496373 | 95496897 | 95496373 | ID=MS.gene72033.t1;Parent=MS.gene72033 |
| chr3.4 | exon | 95496373 | 95496897 | 95496373 | ID=MS.gene72033.t1.exon1;Parent=MS.gene72033.t1 |
| chr3.4 | CDS | 95496373 | 95496897 | 95496373 | ID=cds.MS.gene72033.t1;Parent=MS.gene72033.t1 |
| Gene Sequence |
| Protein sequence |
