AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene72195

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene72195.t1 MTR_2g042370 96.296 270 10 0 1 270 1 270 0.0 521
MS.gene72195.t1 MTR_2g042420 92.395 263 16 2 8 270 4 262 5.05e-179 496
MS.gene72195.t1 MTR_2g042430 88.973 263 28 1 8 270 20 281 3.40e-169 473
MS.gene72195.t1 MTR_8g074270 36.981 265 157 8 10 268 22 282 1.03e-52 176
MS.gene72195.t1 MTR_8g074170 36.364 264 160 7 10 268 22 282 7.63e-50 168
MS.gene72195.t1 MTR_8g074210 36.090 266 158 7 10 268 22 282 3.02e-48 164
MS.gene72195.t1 MTR_8g074320 37.584 149 87 3 81 224 73 220 2.51e-29 110
MS.gene72195.t1 MTR_4g050190 37.209 172 106 2 98 268 128 298 7.16e-29 113
MS.gene72195.t1 MTR_2g099030 33.166 199 125 5 77 268 117 314 5.29e-28 111
MS.gene72195.t1 MTR_7g090310 37.342 158 95 3 114 268 136 292 3.19e-27 108
MS.gene72195.t1 MTR_7g090320 36.709 158 96 3 114 268 137 293 3.79e-26 105
MS.gene72195.t1 MTR_1g075865 42.718 103 58 1 155 257 10 111 3.58e-25 97.4
MS.gene72195.t1 MTR_7g490350 28.966 290 172 10 1 268 1 278 1.39e-24 101
MS.gene72195.t1 MTR_7g490330 37.107 159 97 3 112 268 115 272 2.68e-24 99.8
MS.gene72195.t1 MTR_2g087780 34.810 158 101 2 112 268 146 302 3.27e-24 100
MS.gene72195.t1 MTR_4g112670 33.962 159 102 3 112 268 110 267 3.64e-22 94.4
MS.gene72195.t1 MTR_5g091980 33.663 202 123 4 77 268 73 273 3.91e-22 94.0
MS.gene72195.t1 MTR_7g090300 29.123 285 162 9 10 268 8 278 4.26e-21 91.7
MS.gene72195.t1 MTR_2g058450 33.333 159 100 5 114 268 113 269 6.62e-19 85.1
MS.gene72195.t1 MTR_0003s0530 34.146 123 79 2 147 268 1 122 2.41e-18 81.3
MS.gene72195.t1 MTR_4g112630 32.099 162 104 4 112 268 109 269 2.56e-18 83.6
MS.gene72195.t1 MTR_4g112650 32.484 157 101 4 116 268 113 268 7.35e-18 82.4
MS.gene72195.t1 MTR_2g058410 33.121 157 99 5 116 268 12 166 5.05e-16 75.5
MS.gene72195.t1 MTR_4g112840 34.545 110 69 3 161 268 42 150 9.50e-13 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene72195.t1 AT3G51430 33.588 262 159 6 19 269 20 277 2.31e-44 154
MS.gene72195.t1 AT3G51430 33.716 261 158 6 19 268 20 276 6.10e-44 153
MS.gene72195.t1 AT3G51440 34.100 261 157 6 19 268 20 276 5.74e-43 150
MS.gene72195.t1 AT3G51420 32.593 270 169 7 10 270 13 278 1.56e-41 146
MS.gene72195.t1 AT3G51450 32.616 279 167 8 5 268 4 276 3.00e-41 145
MS.gene72195.t1 AT3G59530 31.544 298 163 11 11 268 20 316 2.24e-31 120
MS.gene72195.t1 AT3G59530 31.544 298 163 11 11 268 20 316 2.24e-31 120
MS.gene72195.t1 AT3G59530 31.544 298 163 11 11 268 20 316 2.24e-31 120
MS.gene72195.t1 AT3G57020 32.381 210 134 7 65 268 54 261 1.04e-26 107
MS.gene72195.t1 AT5G22020 29.924 264 155 6 29 268 45 302 9.27e-26 105
MS.gene72195.t1 AT5G22020 29.924 264 155 6 29 268 45 302 9.27e-26 105
MS.gene72195.t1 AT5G22020 29.924 264 155 6 29 268 45 302 9.27e-26 105
MS.gene72195.t1 AT3G57030 37.107 159 97 3 112 268 124 281 5.97e-25 102
MS.gene72195.t1 AT3G57020 34.731 167 103 5 106 268 111 275 6.11e-25 102
MS.gene72195.t1 AT1G08470 34.177 158 102 2 112 268 140 296 8.17e-25 102
MS.gene72195.t1 AT3G57010 33.125 160 102 4 112 268 119 276 1.14e-22 96.3
MS.gene72195.t1 AT2G41290 35.673 171 101 5 106 268 113 282 1.21e-21 93.2
MS.gene72195.t1 AT2G41300 33.133 166 107 4 106 268 136 300 5.83e-21 91.7
MS.gene72195.t1 AT1G74010 28.947 228 138 6 65 269 38 264 8.58e-19 84.7
MS.gene72195.t1 AT1G74000 25.764 229 145 7 65 269 41 268 2.61e-15 75.1
MS.gene72195.t1 AT1G74020 27.329 161 113 4 112 269 107 266 4.32e-13 68.6

Find 58 sgRNAs with CRISPR-Local

Find 214 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCAATTGTGCATACCAAATT+TGG 0.147851 2.1:-50705147 None:intergenic
GAATGGTTAGGAATGGAAAT+TGG 0.237351 2.1:+50702510 MS.gene72195:CDS
TTCCAGTATAACTGGATCTA+TGG 0.248409 2.1:-50702251 None:intergenic
GTTATCCTTTCACAAGTAAA+TGG 0.270520 2.1:+50702757 MS.gene72195:CDS
CGTTATTGTCCATGATGATT+TGG 0.285126 2.1:+50705238 MS.gene72195:CDS
AAGAAGATAATCTTCATCTT+TGG 0.300360 2.1:-50705285 None:intergenic
TTATCCACACAAACATCTTC+TGG 0.304876 2.1:-50702443 None:intergenic
TTGCTCCTGATGGCTCATTC+TGG 0.309987 2.1:+50705557 MS.gene72195:CDS
AGTGTTCCAAGTACCAAATT+TGG 0.315709 2.1:+50705134 MS.gene72195:CDS
TGTCTGAAACATTGGCTAAA+AGG 0.318273 2.1:+50705469 MS.gene72195:CDS
GAAGGAATTTCCAGTATAAC+TGG 0.333326 2.1:-50702259 None:intergenic
GGAATGGAAATTGGGAGAAT+TGG 0.337340 2.1:+50702519 MS.gene72195:CDS
CCAAATTTGGTATGCACAAT+TGG 0.339387 2.1:+50705147 MS.gene72195:CDS
TGCATACCAAATTTGGTACT+TGG 0.353785 2.1:-50705140 None:intergenic
ACCCATAGATCCAGTTATAC+TGG 0.369921 2.1:+50702249 MS.gene72195:CDS
ATTCTGGATCGCTTTAATTC+AGG 0.374953 2.1:+50705573 MS.gene72195:CDS
TGGTATGCACAATTGGTATC+TGG 0.390581 2.1:+50705154 MS.gene72195:CDS
CATCTCTTGTAGCTGTATAT+AGG 0.396008 2.1:-50702475 None:intergenic
ATAGATAGCTCTTCTTTGCT+TGG 0.400675 2.1:+50702548 MS.gene72195:CDS
GATTCAGGTGTCTGAAACAT+TGG 0.407166 2.1:+50705461 MS.gene72195:intron
GGAGCAAGATTAATGTTATC+AGG 0.407899 2.1:-50705541 None:intergenic
ATCTTCTTGTCTGCGAAACT+TGG 0.421361 2.1:+50705300 MS.gene72195:CDS
AATGGTTAGGAATGGAAATT+GGG 0.437047 2.1:+50702511 MS.gene72195:CDS
AGATGGTTGGATTAAAAGAA+TGG 0.447747 2.1:+50702493 MS.gene72195:CDS
GTTGGATTAAAAGAATGGTT+AGG 0.454450 2.1:+50702498 MS.gene72195:CDS
CTAAAGGTTACAGAAGAAGA+TGG 0.472505 2.1:+50702727 MS.gene72195:CDS
TTTATTGAAAACCTTCCAGC+TGG 0.481363 2.1:+50705517 MS.gene72195:CDS
AACATTAATCTTGCTCCTGA+TGG 0.489893 2.1:+50705547 MS.gene72195:CDS
GAGAACCATTTACTTGTGAA+AGG 0.496653 2.1:-50702762 None:intergenic
ATTAAAAGAATGGTTAGGAA+TGG 0.506704 2.1:+50702503 MS.gene72195:CDS
GCGATCCAGAATGAGCCATC+AGG 0.509038 2.1:-50705562 None:intergenic
TCCAGTATAACTGGATCTAT+GGG 0.509524 2.1:-50702250 None:intergenic
TAACAGAAAGTGATTAAACT+TGG 0.522162 2.1:+50702401 MS.gene72195:intron
TTTGATCAAAACCTCAAGCT+TGG 0.522434 2.1:+50702180 MS.gene72195:CDS
CAAATCATCATGGACAATAA+CGG 0.527432 2.1:-50705237 None:intergenic
GATGATTTGGCCTTTGCCAA+TGG 0.528631 2.1:+50705251 MS.gene72195:CDS
TTTGGACAGTGCAACACCAT+TGG 0.529499 2.1:-50705267 None:intergenic
GGAATCACAACATCTAAAGA+TGG 0.552529 2.1:+50702569 MS.gene72195:CDS
AAAGTGATTAAACTTGGAGA+AGG 0.557723 2.1:+50702407 MS.gene72195:CDS
TGGCAAAGGCCAAATCATCA+TGG 0.559095 2.1:-50705247 None:intergenic
GAAGTTGAAGATGCAGCAGA+AGG 0.562477 2.1:-50702277 None:intergenic
AGCTGCTAGCACCAAGCTTG+AGG 0.565489 2.1:-50702191 None:intergenic
TGTTATCAGGTCCAGCTGGA+AGG 0.568452 2.1:-50705528 None:intergenic
ATACAGCTACAAGAGATGGT+TGG 0.570015 2.1:+50702480 MS.gene72195:CDS
TTAATGTTATCAGGTCCAGC+TGG 0.572564 2.1:-50705532 None:intergenic
CATTGTCTGTGATGCTACCA+AGG 0.585980 2.1:+50702598 MS.gene72195:CDS
CTATATACAGCTACAAGAGA+TGG 0.586350 2.1:+50702476 MS.gene72195:CDS
GTGCTTGAGGCAAGACCACA+TGG 0.588367 2.1:+50705179 MS.gene72195:CDS
GATGTAATAGAAGCATCAGA+TGG 0.591553 2.1:+50705098 MS.gene72195:CDS
ATAACGGTTTCATTTGACAT+GGG 0.603716 2.1:-50705221 None:intergenic
AATAACGGTTTCATTTGACA+TGG 0.605627 2.1:-50705222 None:intergenic
GATGTTTGTGTGGATAAAGA+TGG 0.610609 2.1:+50702449 MS.gene72195:CDS
CAGTGCAACACCATTGGCAA+AGG 0.613191 2.1:-50705261 None:intergenic
TACCTTAAAAGTTGTCCATG+TGG 0.628210 2.1:-50705194 None:intergenic
ATCACAACATCTAAAGATGG+TGG 0.643963 2.1:+50702572 MS.gene72195:CDS
TGGCTAAAAGGAATAAACAA+AGG 0.651228 2.1:+50705481 MS.gene72195:CDS
TTGGTATCTGGATGTGCTTG+AGG 0.671766 2.1:+50705166 MS.gene72195:CDS
GCAACCAGAAGATGTTTGTG+TGG 0.684744 2.1:+50702439 MS.gene72195:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTAATTTTATTATCCTTAA+AGG + chr2.1:50704431-50704450 MS.gene72195:intron 10.0%
!!! TAAATAATTTTTTTTACAGA+GGG - chr2.1:50704586-50704605 None:intergenic 10.0%
!!! TCTTTAAATATAATTTTAGA+TGG + chr2.1:50703744-50703763 MS.gene72195:intron 10.0%
!!! TTAATATTTTTTTGAAAACT+CGG - chr2.1:50703582-50703601 None:intergenic 10.0%
!!! TTCAAACAATATTATTTTAA+GGG + chr2.1:50704515-50704534 MS.gene72195:intron 10.0%
!! AAAAAAATTATGAAAGTTAC+AGG - chr2.1:50702637-50702656 None:intergenic 15.0%
!! AAAGTTATCAATTTAATTCT+AGG + chr2.1:50703094-50703113 MS.gene72195:intron 15.0%
!! AAGATTAAATTCCTAATTAA+AGG + chr2.1:50705349-50705368 MS.gene72195:intron 15.0%
!! ACACAAAACAATTAAAATAA+GGG - chr2.1:50704898-50704917 None:intergenic 15.0%
!! TACAATGATCATTAATAAAA+GGG + chr2.1:50704478-50704497 MS.gene72195:intron 15.0%
!! TATATTTAAAGAATGTTTCA+AGG - chr2.1:50703736-50703755 None:intergenic 15.0%
!!! AAAAAACAAACTTACTTTTT+TGG + chr2.1:50703663-50703682 MS.gene72195:intron 15.0%
!!! AAATAATTTTTTTTACAGAG+GGG - chr2.1:50704585-50704604 None:intergenic 15.0%
!!! AATTTTTTTTTTTTACCAAC+AGG + chr2.1:50702648-50702667 MS.gene72195:intron 15.0%
!!! ATTTGAAGATTTTTTGATTT+TGG - chr2.1:50704792-50704811 None:intergenic 15.0%
!!! CTAAATAATTTTTTTTACAG+AGG - chr2.1:50704587-50704606 None:intergenic 15.0%
!!! CTTCAAACAATATTATTTTA+AGG + chr2.1:50704514-50704533 MS.gene72195:intron 15.0%
!!! TAAACAATTTTTTTTACAGA+GGG + chr2.1:50704827-50704846 MS.gene72195:intron 15.0%
!! ACAAAAAATCTTCAAATTAC+AGG - chr2.1:50704618-50704637 None:intergenic 20.0%
!! ACAATGATCATTAATAAAAG+GGG + chr2.1:50704479-50704498 MS.gene72195:intron 20.0%
!! CAAAAAATCTTCAAATTACA+GGG + chr2.1:50704796-50704815 MS.gene72195:intron 20.0%
!! CAAAAAATCTTCAAATTACA+GGG - chr2.1:50704815-50704796 None:intergenic 20.0%
!! CTCATTATAATTATCCTTTA+AGG - chr2.1:50704448-50704467 None:intergenic 20.0%
!! GACACAAAACAATTAAAATA+AGG - chr2.1:50704899-50704918 None:intergenic 20.0%
!! GTACAATGATCATTAATAAA+AGG + chr2.1:50704477-50704496 MS.gene72195:intron 20.0%
!! TAGAGATGAATTATTTGTTT+GGG - chr2.1:50702801-50702820 None:intergenic 20.0%
!! TCAAAAAATCTTCAAATTAC+AGG + chr2.1:50704795-50704814 MS.gene72195:intron 20.0%
!!! AAACAATTTTTTTTACAGAG+GGG + chr2.1:50704828-50704847 MS.gene72195:intron 20.0%
!!! AATCATGCAGTATTTTATAT+TGG - chr2.1:50702675-50702694 None:intergenic 20.0%
!!! ATAAAATTTTCACCTGTAAT+TGG - chr2.1:50702318-50702337 None:intergenic 20.0%
!!! ATACATATTATTTTGAACAC+TGG - chr2.1:50702353-50702372 None:intergenic 20.0%
!!! CGTTTTATTTTTTTTTGAAG+TGG + chr2.1:50704283-50704302 MS.gene72195:intron 20.0%
!!! CTAAACAATTTTTTTTACAG+AGG + chr2.1:50704826-50704845 MS.gene72195:intron 20.0%
!!! GATCTACTAAACAAATTTTT+TGG - chr2.1:50703862-50703881 None:intergenic 20.0%
!!! TAACTGATAAATTTTTATGC+AGG + chr2.1:50703945-50703964 MS.gene72195:intron 20.0%
!!! TTGTTTTGTGTCAATAAATT+TGG + chr2.1:50704907-50704926 MS.gene72195:intron 20.0%
! AAAAGACCAAATATCTTTCA+TGG - chr2.1:50704128-50704147 None:intergenic 25.0%
! AACGATAATTTAAATGAGAG+AGG - chr2.1:50704267-50704286 None:intergenic 25.0%
! AAGAAGATAATCTTCATCTT+TGG - chr2.1:50705288-50705307 None:intergenic 25.0%
! AATGTTTCAAGGATATTCAT+TGG - chr2.1:50703725-50703744 None:intergenic 25.0%
! ACGATAATTTAAATGAGAGA+GGG - chr2.1:50704266-50704285 None:intergenic 25.0%
! ATCAAAGATCGAAAACAATT+AGG + chr2.1:50704366-50704385 MS.gene72195:intron 25.0%
! ATTAAAAGAATGGTTAGGAA+TGG + chr2.1:50702503-50702522 MS.gene72195:CDS 25.0%
! ATTACACAATATTGTTGATG+AGG + chr2.1:50702988-50703007 MS.gene72195:intron 25.0%
! GACGTATTTATCCTTTAATT+AGG - chr2.1:50705363-50705382 None:intergenic 25.0%
! GGCATCAAATTCAAAATTTA+AGG - chr2.1:50704761-50704780 None:intergenic 25.0%
! GTAGAGATGAATTATTTGTT+TGG - chr2.1:50702802-50702821 None:intergenic 25.0%
! TAACAGAAAGTGATTAAACT+TGG + chr2.1:50702401-50702420 MS.gene72195:intron 25.0%
! TTTCTAAACACTTATTGTAC+TGG - chr2.1:50704343-50704362 None:intergenic 25.0%
!! AATCACTTTCTGTTATGTTT+TGG - chr2.1:50702396-50702415 None:intergenic 25.0%
!! TATTGTGTAATGTTCTTTTG+AGG - chr2.1:50702979-50702998 None:intergenic 25.0%
!!! ATTTATTCTGAGATTCATGT+TGG - chr2.1:50703319-50703338 None:intergenic 25.0%
!!! GGAAGTAAGTAGAAATTTTT+TGG + chr2.1:50705321-50705340 MS.gene72195:intron 25.0%
!!! TATGCAGGAAAATTACTTTT+TGG + chr2.1:50703960-50703979 MS.gene72195:intron 25.0%
!!! TGAGTGTTTTAAAGACATTA+AGG + chr2.1:50703907-50703926 MS.gene72195:intron 25.0%
!!! TTCTAATTTTTTAGTGAACC+AGG + chr2.1:50705066-50705085 MS.gene72195:intron 25.0%
AAACCGAAATATCGATGATA+TGG + chr2.1:50704690-50704709 MS.gene72195:intron 30.0%
AAAGTGATTAAACTTGGAGA+AGG + chr2.1:50702407-50702426 MS.gene72195:CDS 30.0%
AAATATCGATGATATGGTAG+GGG + chr2.1:50704696-50704715 MS.gene72195:intron 30.0%
AAATCTTCAAATTACAGGGT+TGG + chr2.1:50704800-50704819 MS.gene72195:intron 30.0%
AAATCTTCAAATTACAGGGT+TGG - chr2.1:50704819-50704800 None:intergenic 30.0%
AATAACGGTTTCATTTGACA+TGG - chr2.1:50705225-50705244 None:intergenic 30.0%
AATGGTTAGGAATGGAAATT+GGG + chr2.1:50702511-50702530 MS.gene72195:CDS 30.0%
AGAGAAAACAAAAACTCTGA+AGG - chr2.1:50704184-50704203 None:intergenic 30.0%
AGATGGTTGGATTAAAAGAA+TGG + chr2.1:50702493-50702512 MS.gene72195:CDS 30.0%
ATAACGGTTTCATTTGACAT+GGG - chr2.1:50705224-50705243 None:intergenic 30.0%
ATCCAAAAACAACCAATTAC+AGG + chr2.1:50702303-50702322 MS.gene72195:CDS 30.0%
CAAATCATCATGGACAATAA+CGG - chr2.1:50705240-50705259 None:intergenic 30.0%
CCTGTAATATTAGTGATTTC+CGG + chr2.1:50704555-50704574 MS.gene72195:intron 30.0%
CCTGTAATTTGAGAGAATTT+CGG - chr2.1:50704728-50704747 None:intergenic 30.0%
CGAAATTCTCTCAAATTACA+GGG + chr2.1:50704726-50704745 MS.gene72195:intron 30.0%
CGATAATTTAAATGAGAGAG+GGG - chr2.1:50704265-50704284 None:intergenic 30.0%
CGGAAATCACTAATATTACA+GGG - chr2.1:50704557-50704576 None:intergenic 30.0%
CTACCATATCATCGATATTT+CGG - chr2.1:50704696-50704715 None:intergenic 30.0%
GAAATATCGATGATATGGTA+GGG + chr2.1:50704695-50704714 MS.gene72195:intron 30.0%
GAAATCACTAATATTACAGG+GGG - chr2.1:50704555-50704574 None:intergenic 30.0%
GAAATTCTCTCAAATTACAG+GGG + chr2.1:50704727-50704746 MS.gene72195:intron 30.0%
GGAAATCACTAATATTACAG+GGG - chr2.1:50704556-50704575 None:intergenic 30.0%
GTTATCCTTTCACAAGTAAA+TGG + chr2.1:50702757-50702776 MS.gene72195:CDS 30.0%
GTTGGATTAAAAGAATGGTT+AGG + chr2.1:50702498-50702517 MS.gene72195:CDS 30.0%
TGGCTAAAAGGAATAAACAA+AGG + chr2.1:50705481-50705500 MS.gene72195:CDS 30.0%
TGTACGGTAAAAATTCATGA+AGG - chr2.1:50704647-50704666 None:intergenic 30.0%
! AAAATTGATGATATGGCAGA+GGG + chr2.1:50704666-50704685 MS.gene72195:intron 30.0%
! AATTTGGTAGCCTTAAGAAA+AGG + chr2.1:50704923-50704942 MS.gene72195:intron 30.0%
! ACCGTACAAAATTGATGATA+TGG + chr2.1:50704659-50704678 MS.gene72195:intron 30.0%
! CTTTTACCAAGGAGTTAAAA+TGG + chr2.1:50702874-50702893 MS.gene72195:intron 30.0%
! GCCATATCATCAATTTTGTA+CGG - chr2.1:50704663-50704682 None:intergenic 30.0%
! GTATTTTATATTGGACCTGT+TGG - chr2.1:50702666-50702685 None:intergenic 30.0%
! TTCTCTTTTGTGTTAGAGAT+TGG - chr2.1:50703073-50703092 None:intergenic 30.0%
! TTTTCTTGTGAATCAGATTC+AGG + chr2.1:50705446-50705465 MS.gene72195:intron 30.0%
! TTTTGAATTTGATGCCATGA+AGG + chr2.1:50704765-50704784 MS.gene72195:intron 30.0%
!! TTCTTTTGACGTATTTCTGT+AGG + chr2.1:50703155-50703174 MS.gene72195:intron 30.0%
!!! CATTTTAACTCCTTGGTAAA+AGG - chr2.1:50702876-50702895 None:intergenic 30.0%
!!! TTTTTGATTTTGGACCTTCA+TGG - chr2.1:50704782-50704801 None:intergenic 30.0%
AACATTAATCTTGCTCCTGA+TGG + chr2.1:50705547-50705566 MS.gene72195:CDS 35.0%
AATATCGATGATATGGTAGG+GGG + chr2.1:50704697-50704716 MS.gene72195:intron 35.0%
AGTGTTCCAAGTACCAAATT+TGG + chr2.1:50705134-50705153 MS.gene72195:CDS 35.0%
ATAGATAGCTCTTCTTTGCT+TGG + chr2.1:50702548-50702567 MS.gene72195:CDS 35.0%
ATCACAACATCTAAAGATGG+TGG + chr2.1:50702572-50702591 MS.gene72195:CDS 35.0%
ATGACGTTGTGACAATAAAG+AGG - chr2.1:50703027-50703046 None:intergenic 35.0%
CAATAATCGACTAATCCGAA+GGG - chr2.1:50703550-50703569 None:intergenic 35.0%
CACTGACCATGAAAGATATT+TGG + chr2.1:50704119-50704138 MS.gene72195:intron 35.0%
CAGGCTTGATTTAATTACCT+TGG - chr2.1:50702618-50702637 None:intergenic 35.0%
CATCTCTTGTAGCTGTATAT+AGG - chr2.1:50702478-50702497 None:intergenic 35.0%
CCAAATTTGGTATGCACAAT+TGG + chr2.1:50705147-50705166 MS.gene72195:CDS 35.0%
CCAATTGTGCATACCAAATT+TGG - chr2.1:50705150-50705169 None:intergenic 35.0%
CCGAAATTCTCTCAAATTAC+AGG + chr2.1:50704725-50704744 MS.gene72195:intron 35.0%
CCGGAAATCACTAATATTAC+AGG - chr2.1:50704558-50704577 None:intergenic 35.0%
CCTGTAATATTAGCGATTTC+CGG - chr2.1:50704858-50704877 None:intergenic 35.0%
CGAAATATCGATGATATGGT+AGG + chr2.1:50704694-50704713 MS.gene72195:intron 35.0%
CGGAAATCGCTAATATTACA+GGG + chr2.1:50704856-50704875 MS.gene72195:intron 35.0%
CGTTATTGTCCATGATGATT+TGG + chr2.1:50705238-50705257 MS.gene72195:CDS 35.0%
CTAAAGGTTACAGAAGAAGA+TGG + chr2.1:50702727-50702746 MS.gene72195:CDS 35.0%
CTATATACAGCTACAAGAGA+TGG + chr2.1:50702476-50702495 MS.gene72195:CDS 35.0%
GAAATCGCTAATATTACAGG+GGG + chr2.1:50704858-50704877 MS.gene72195:intron 35.0%
GAAGGAATTTCCAGTATAAC+TGG - chr2.1:50702262-50702281 None:intergenic 35.0%
GAATGGTTAGGAATGGAAAT+TGG + chr2.1:50702510-50702529 MS.gene72195:CDS 35.0%
GAGAACCATTTACTTGTGAA+AGG - chr2.1:50702765-50702784 None:intergenic 35.0%
GATGTTTGTGTGGATAAAGA+TGG + chr2.1:50702449-50702468 MS.gene72195:CDS 35.0%
GGAAATCGCTAATATTACAG+GGG + chr2.1:50704857-50704876 MS.gene72195:intron 35.0%
GGAATCACAACATCTAAAGA+TGG + chr2.1:50702569-50702588 MS.gene72195:CDS 35.0%
GGAGCAAGATTAATGTTATC+AGG - chr2.1:50705544-50705563 None:intergenic 35.0%
TACCTTAAAAGTTGTCCATG+TGG - chr2.1:50705197-50705216 None:intergenic 35.0%
TATTACATCATCTGCAAACC+TGG - chr2.1:50705087-50705106 None:intergenic 35.0%
TATTGTCACAACGTCATTCT+AGG + chr2.1:50703030-50703049 MS.gene72195:intron 35.0%
TCCAGTATAACTGGATCTAT+GGG - chr2.1:50702253-50702272 None:intergenic 35.0%
TGCATACCAAATTTGGTACT+TGG - chr2.1:50705143-50705162 None:intergenic 35.0%
TTATCCACACAAACATCTTC+TGG - chr2.1:50702446-50702465 None:intergenic 35.0%
TTCCAGTATAACTGGATCTA+TGG - chr2.1:50702254-50702273 None:intergenic 35.0%
TTTATTGAAAACCTTCCAGC+TGG + chr2.1:50705517-50705536 MS.gene72195:CDS 35.0%
TTTGATCAAAACCTCAAGCT+TGG + chr2.1:50702180-50702199 MS.gene72195:CDS 35.0%
! AAGTTGCATGGTGCATTTTA+CGG + chr2.1:50704207-50704226 MS.gene72195:intron 35.0%
! CAAAATTGATGATATGGCAG+AGG + chr2.1:50704665-50704684 MS.gene72195:intron 35.0%
! TGCACATTTACCTTTTACCA+AGG + chr2.1:50702863-50702882 MS.gene72195:intron 35.0%
! TGTCTGAAACATTGGCTAAA+AGG + chr2.1:50705469-50705488 MS.gene72195:CDS 35.0%
! TTGTGTTAGAGATTGGTTCA+AGG - chr2.1:50703066-50703085 None:intergenic 35.0%
!! CACCTGTAATTGGTTGTTTT+TGG - chr2.1:50702308-50702327 None:intergenic 35.0%
!! GATGTAATAGAAGCATCAGA+TGG + chr2.1:50705098-50705117 MS.gene72195:CDS 35.0%
!! GCTTGAGGTTTTGATCAAAT+TGG - chr2.1:50702179-50702198 None:intergenic 35.0%
!! GGATTAGTCGATTATTGGAT+CGG + chr2.1:50703553-50703572 MS.gene72195:intron 35.0%
!! TTTCACAACTTGCTGAAAGT+TGG + chr2.1:50703274-50703293 MS.gene72195:intron 35.0%
!!! CTTTCTCCATTTTAACTCCT+TGG - chr2.1:50702883-50702902 None:intergenic 35.0%
!!! GCATAACCTTAGTTTTAACG+CGG - chr2.1:50703884-50703903 None:intergenic 35.0%
!!! TTTTTTACAGAGGGGTAAAC+CGG + chr2.1:50704836-50704855 MS.gene72195:intron 35.0%
!!! TTTTTTACAGAGGGGTAAAC+CGG - chr2.1:50704855-50704836 None:intergenic 35.0%
AACTCAAGCTCAACCCATTT+GGG + chr2.1:50703432-50703451 MS.gene72195:intron 40.0%
ACCCATAGATCCAGTTATAC+TGG + chr2.1:50702249-50702268 MS.gene72195:CDS 40.0%
AGTGTTTGCAACATTTGAGC+AGG - chr2.1:50704952-50704971 None:intergenic 40.0%
ATACAGCTACAAGAGATGGT+TGG + chr2.1:50702480-50702499 MS.gene72195:CDS 40.0%
ATCTTCTTGTCTGCGAAACT+TGG + chr2.1:50705300-50705319 MS.gene72195:CDS 40.0%
CAAGTAGTGTTCATATGTGG+TGG - chr2.1:50702910-50702929 None:intergenic 40.0%
CCAATAATCGACTAATCCGA+AGG - chr2.1:50703551-50703570 None:intergenic 40.0%
CCGGAAATCGCTAATATTAC+AGG + chr2.1:50704855-50704874 MS.gene72195:intron 40.0%
GCAAGTTGTGAAACGTTTGT+CGG - chr2.1:50703267-50703286 None:intergenic 40.0%
GCTCAAGTAGTGTTCATATG+TGG - chr2.1:50702913-50702932 None:intergenic 40.0%
GGAATGGAAATTGGGAGAAT+TGG + chr2.1:50702519-50702538 MS.gene72195:CDS 40.0%
GTAGATCCGCGTTAAAACTA+AGG + chr2.1:50703875-50703894 MS.gene72195:intron 40.0%
GTGTTTGCAACATTTGAGCA+GGG - chr2.1:50704951-50704970 None:intergenic 40.0%
TAACTCAAGCTCAACCCATT+TGG + chr2.1:50703431-50703450 MS.gene72195:intron 40.0%
TGCAAAATGTAACGACGTGT+CGG - chr2.1:50702700-50702719 None:intergenic 40.0%
TGGTATGCACAATTGGTATC+TGG + chr2.1:50705154-50705173 MS.gene72195:CDS 40.0%
TTAATGTTATCAGGTCCAGC+TGG - chr2.1:50705535-50705554 None:intergenic 40.0%
! ACACGTCGTTACATTTTGCA+GGG + chr2.1:50702700-50702719 MS.gene72195:intron 40.0%
! ATTCCATCTGGTGCCAATTT+CGG + chr2.1:50703514-50703533 MS.gene72195:intron 40.0%
! CTTGTGAAGTGTCTTGCTTA+AGG + chr2.1:50704079-50704098 MS.gene72195:intron 40.0%
! GACCACATGGACAACTTTTA+AGG + chr2.1:50705192-50705211 MS.gene72195:CDS 40.0%
! GATTCAGGTGTCTGAAACAT+TGG + chr2.1:50705461-50705480 MS.gene72195:intron 40.0%
! GTTTTCTCTTGCAAGTTGCA+TGG + chr2.1:50704195-50704214 MS.gene72195:intron 40.0%
! TGAAGTGTCTTGCTTAAGGT+GGG + chr2.1:50704083-50704102 MS.gene72195:intron 40.0%
!! CCTTCGGATTAGTCGATTAT+TGG + chr2.1:50703548-50703567 MS.gene72195:intron 40.0%
!!! ATCGGTTTTTGGGTGCATTT+CGG - chr2.1:50704151-50704170 None:intergenic 40.0%
!!! CAAAGCACTGATCGGTTTTT+GGG - chr2.1:50704161-50704180 None:intergenic 40.0%
!!! CATTTTGCAGGGTTTGCTAA+AGG + chr2.1:50702711-50702730 MS.gene72195:intron 40.0%
ACTCAAGCTCAACCCATTTG+GGG + chr2.1:50703433-50703452 MS.gene72195:intron 45.0%
CATTGTCTGTGATGCTACCA+AGG + chr2.1:50702598-50702617 MS.gene72195:CDS 45.0%
GAAGTTGAAGATGCAGCAGA+AGG - chr2.1:50702280-50702299 None:intergenic 45.0%
GCAACCAGAAGATGTTTGTG+TGG + chr2.1:50702439-50702458 MS.gene72195:CDS 45.0%
TGGCAAAGGCCAAATCATCA+TGG - chr2.1:50705250-50705269 None:intergenic 45.0%
TTGGTATCTGGATGTGCTTG+AGG + chr2.1:50705166-50705185 MS.gene72195:CDS 45.0%
TTTGGACAGTGCAACACCAT+TGG - chr2.1:50705270-50705289 None:intergenic 45.0%
! GACACGTCGTTACATTTTGC+AGG + chr2.1:50702699-50702718 MS.gene72195:intron 45.0%
! GATGATTTGGCCTTTGCCAA+TGG + chr2.1:50705251-50705270 MS.gene72195:CDS 45.0%
! GTGAAGTGTCTTGCTTAAGG+TGG + chr2.1:50704082-50704101 MS.gene72195:intron 45.0%
!! GAGCAGGGTACCTTTTCTTA+AGG - chr2.1:50704936-50704955 None:intergenic 45.0%
!! GTTTCAGGTTCGATTCCATC+TGG + chr2.1:50703502-50703521 MS.gene72195:intron 45.0%
!! TCTGAAGGCAAAGCACTGAT+CGG - chr2.1:50704169-50704188 None:intergenic 45.0%
!!! GCAAAGCACTGATCGGTTTT+TGG - chr2.1:50704162-50704181 None:intergenic 45.0%
!!! TTATTATATATATTTTTGTT+TGG - chr2.1:50702935-50702954 None:intergenic 5.0%
AAGATCCCAAGCCAACCACT+AGG - chr2.1:50703463-50703482 None:intergenic 50.0%
ATCGAACCTGAAACCTCTGG+AGG - chr2.1:50703496-50703515 None:intergenic 50.0%
CAGTGCAACACCATTGGCAA+AGG - chr2.1:50705264-50705283 None:intergenic 50.0%
GGAATCGAACCTGAAACCTC+TGG - chr2.1:50703499-50703518 None:intergenic 50.0%
GGGACCAATTCCACCGAAAT+TGG - chr2.1:50703530-50703549 None:intergenic 50.0%
GGTGCCAATTTCGGTGGAAT+TGG + chr2.1:50703523-50703542 MS.gene72195:intron 50.0%
TGTTATCAGGTCCAGCTGGA+AGG - chr2.1:50705531-50705550 None:intergenic 50.0%
TTAAGGTGGGACCAGAGAGT+AGG + chr2.1:50704096-50704115 MS.gene72195:intron 50.0%
TTCGGTGGAATTGGTCCCTT+CGG + chr2.1:50703532-50703551 MS.gene72195:intron 50.0%
TTGCTCCTGATGGCTCATTC+TGG + chr2.1:50705557-50705576 MS.gene72195:CDS 50.0%
TTTGCTCCTCCAGAGGTTTC+AGG + chr2.1:50703487-50703506 MS.gene72195:intron 50.0%
! AAGCTCAACCCATTTGGGGT+TGG + chr2.1:50703437-50703456 MS.gene72195:intron 50.0%
!! TAGTGGTTGGCTTGGGATCT+TGG + chr2.1:50703462-50703481 MS.gene72195:intron 50.0%
ACCACTAGGCCAACCCCAAA+TGG - chr2.1:50703449-50703468 None:intergenic 55.0%
AGCTGCTAGCACCAAGCTTG+AGG - chr2.1:50702194-50702213 None:intergenic 55.0%
CATGGTCAGTGCCTACTCTC+TGG - chr2.1:50704110-50704129 None:intergenic 55.0%
CCACCGAAATTGGCACCAGA+TGG - chr2.1:50703520-50703539 None:intergenic 55.0%
CCACTAGGCCAACCCCAAAT+GGG - chr2.1:50703448-50703467 None:intergenic 55.0%
GCGATCCAGAATGAGCCATC+AGG - chr2.1:50705565-50705584 None:intergenic 55.0%
GTGCTTGAGGCAAGACCACA+TGG + chr2.1:50705179-50705198 MS.gene72195:CDS 55.0%
! CCATCTGGTGCCAATTTCGG+TGG + chr2.1:50703517-50703536 MS.gene72195:intron 55.0%
! CTTGGAGTTTGCTCCTCCAG+AGG + chr2.1:50703480-50703499 MS.gene72195:intron 55.0%
!! GTTGGCCTAGTGGTTGGCTT+GGG + chr2.1:50703455-50703474 MS.gene72195:intron 55.0%
!! TTTGGGGTTGGCCTAGTGGT+TGG + chr2.1:50703449-50703468 MS.gene72195:intron 55.0%
!! CCCATTTGGGGTTGGCCTAG+TGG + chr2.1:50703445-50703464 MS.gene72195:intron 60.0%
!! GGTTGGCCTAGTGGTTGGCT+TGG + chr2.1:50703454-50703473 MS.gene72195:intron 60.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 50702169 50705594 50702169 ID=MS.gene72195
chr2.1 mRNA 50702169 50705594 50702169 ID=MS.gene72195.t1;Parent=MS.gene72195
chr2.1 exon 50702169 50702324 50702169 ID=MS.gene72195.t1.exon1;Parent=MS.gene72195.t1
chr2.1 CDS 50702169 50702324 50702169 ID=cds.MS.gene72195.t1;Parent=MS.gene72195.t1
chr2.1 exon 50702407 50702619 50702407 ID=MS.gene72195.t1.exon2;Parent=MS.gene72195.t1
chr2.1 CDS 50702407 50702619 50702407 ID=cds.MS.gene72195.t1;Parent=MS.gene72195.t1
chr2.1 exon 50702721 50702791 50702721 ID=MS.gene72195.t1.exon3;Parent=MS.gene72195.t1
chr2.1 CDS 50702721 50702791 50702721 ID=cds.MS.gene72195.t1;Parent=MS.gene72195.t1
chr2.1 exon 50705088 50705324 50705088 ID=MS.gene72195.t1.exon4;Parent=MS.gene72195.t1
chr2.1 CDS 50705088 50705324 50705088 ID=cds.MS.gene72195.t1;Parent=MS.gene72195.t1
chr2.1 exon 50705462 50705594 50705462 ID=MS.gene72195.t1.exon5;Parent=MS.gene72195.t1
chr2.1 CDS 50705462 50705594 50705462 ID=cds.MS.gene72195.t1;Parent=MS.gene72195.t1
Gene Sequence

>MS.gene72195

ATGGCTTCCAATTTGATCAAAACCTCAAGCTTGGTGCTAGCAGCTTTAGTTGCTCTCACAGTTCAAGTCTTCTACTTCTCACCCATAGATCCAGTTATACTGGAAATTCCTTCTGCTGCATCTTCAACTTCTTCATCCAAAAACAACCAATTACAGGTGAAAATTTTATCAACAATTATAGCCAGTGTTCAAAATAATATGTATTTAATTGTCTATGATTGTTTCCAAAACATAACAGAAAGTGATTAAACTTGGAGAAGGATTTCTGAAGCAACCAGAAGATGTTTGTGTGGATAAAGATGGTGTCCTATATACAGCTACAAGAGATGGTTGGATTAAAAGAATGGTTAGGAATGGAAATTGGGAGAATTGGAAGCATATAGATAGCTCTTCTTTGCTTGGAATCACAACATCTAAAGATGGTGGTCTCATTGTCTGTGATGCTACCAAGGTAATTAAATCAAGCCTGTAACTTTCATAATTTTTTTTTTTTACCAACAGGTCCAATATAAAATACTGCATGATTAACCGACACGTCGTTACATTTTGCAGGGTTTGCTAAAGGTTACAGAAGAAGATGGTTTCAGTGTTATCCTTTCACAAGTAAATGGTTCTCAATTAATGTGAGTCCCAAACAAATAATTCATCTCTACATTTCTTTGTTCTGTTCTTTCATGCTTTGAGACAATTTATTTGCACATTTACCTTTTACCAAGGAGTTAAAATGGAGAAAGTTTTCCACCACATATGAACACTACTTGAGCCAAACAAAAATATATATAATAACAAATTATTGAGTTTCAGTTTCCTCAAAAGAACATTACACAATATTGTTGATGAGGATCTCTAGTTATTCCTCTTTATTGTCACAACGTCATTCTAGGCAGTATACTTCCTTGAACCAATCTCTAACACAAAAGAGAAAAAAGTTATCAATTTAATTCTAGGCAATAACTTTTTCTCTTTCTTTAAATCAAACTAACACTTTCTTTTGACGTATTTCTGTAGGACACTTCTATAGTTATCATTTTTTTGTTGTTCAAATATTGTTACTTAGAAAAAGAAAAATACACACTTTTGAAGATAAAAATGATGCCGACAAACGTTTCACAACTTGCTGAAAGTTGGTGTCAAAAATGACTTCATTCCAACATGAATCTCAGAATAAATATCGCATCATATTAAACTTATGTGACAATAAAATCAACTTAAACTCTTCAATGATTTTTACAACAAAAAAAAATTGAACAAAACTAAAACATTAACTCAAGCTCAACCCATTTGGGGTTGGCCTAGTGGTTGGCTTGGGATCTTGGAGTTTGCTCCTCCAGAGGTTTCAGGTTCGATTCCATCTGGTGCCAATTTCGGTGGAATTGGTCCCTTCGGATTAGTCGATTATTGGATCGGATACCGAGTTTTCAAAAAAATATTAACTCAAGCTCTAAGAATTTTTTAATTAGAAATGAAAAGAAAGTGTAATTTATATATATAAAAAAAAAACAAACTTACTTTTTTGGTGAAAAAAGAAACTTGATTTTTTATAAAAGAAAATGCCAATGAATATCCTTGAAACATTCTTTAAATATAATTTTAGATGGTTTGTAAATTGTAATGTATTCAATACATTAAATAAAACGTAATATTTAATTTTAGAGAATAAATTTTAAATTTTGAGTTTTTAAAGAGTGCTCCAAAAAATTTGTTTAGTAGATCCGCGTTAAAACTAAGGTTATGCTAATGAGTGTTTTAAAGACATTAAGGATTTAAAAACAAAAATAACTGATAAATTTTTATGCAGGAAAATTACTTTTTGGTATTTAAATGTTTTGAATGCATAATTTTTAAGATAAATTTACACTTTAAACTTCTAAATTTGTTAGTATTCTCGTAAAACTAAAATATACAGAAATCTTGTGAAGTGTCTTGCTTAAGGTGGGACCAGAGAGTAGGCACTGACCATGAAAGATATTTGGTCTTTTCCGAAATGCACCCAAAAACCGATCAGTGCTTTGCCTTCAGAGTTTTTGTTTTCTCTTGCAAGTTGCATGGTGCATTTTACGGTTTCAGATAGAATCTTTTCAAATTAGTCTTTACCCCTCTCTCATTTAAATTATCGTTTTATTTTTTTTTGAAGTGGCTAAAATGAAATATATTATCATAATAAGTGTCAACCAGTACAATAAGTGTTTAGAAAGACATCAAAGATCGAAAACAATTAGGAAGACACTAACTACAATTCATATGAATGATTTAGTAAATGATATTAATTTTATTATCCTTAAAGGATAATTATAATGAGACGAATGAAGTACAATGATCATTAATAAAAGGGGATTAAAAAATTTCTTCAAACAATATTATTTTAAGGGTTAATATATGTTTACCCCCTGTAATATTAGTGATTTCCGGTTTACCCCTCTGTAAAAAAAATTATTTAGATTCCAACCCTGTAATTTGAAGATTTTTTGTTTTAGACCTTCATGAATTTTTACCGTACAAAATTGATGATATGGCAGAGGGTAAACCGAAATATCGATGATATGGTAGGGGGTTAAACCGAAATTCTCTCAAATTACAGGGGCAAAAATACCTTAAATTTTGAATTTGATGCCATGAAGGTCCAAAATCAAAAAATCTTCAAATTACAGGGTTGGAATCTAAACAATTTTTTTTACAGAGGGGTAAACCGGAAATCGCTAATATTACAGGGGGTAAACATATATTAACCCTTATTTTAATTGTTTTGTGTCAATAAATTTGGTAGCCTTAAGAAAAGGTACCCTGCTCAAATGTTGCAAACACTCACTAGCTATGAAACTCACAAGTTTGTAAACAAATATGAAAGTCATAAGCTATTATAACTATTTTTTTTTTTATTAAAAAACAAACTATGAGTTTTCTAATTTTTTAGTGAACCAGGTTTGCAGATGATGTAATAGAAGCATCAGATGGAAATATTTATTTCAGTGTTCCAAGTACCAAATTTGGTATGCACAATTGGTATCTGGATGTGCTTGAGGCAAGACCACATGGACAACTTTTAAGGTATAATCCCATGTCAAATGAAACCGTTATTGTCCATGATGATTTGGCCTTTGCCAATGGTGTTGCACTGTCCAAAGATGAAGATTATCTTCTTGTCTGCGAAACTTGGAAGTAAGTAGAAATTTTTTGGTAAAAAAGATTAAATTCCTAATTAAAGGATAAATACGTCAATTAGTTTGTCTTAATAATTTAATTAATATTGTCTTAACAAATTTCATTTTGTATGATTTGTTTTTCTTGTGAATCAGATTCAGGTGTCTGAAACATTGGCTAAAAGGAATAAACAAAGGTAAAACAAATATCTTTATTGAAAACCTTCCAGCTGGACCTGATAACATTAATCTTGCTCCTGATGGCTCATTCTGGATCGCTTTAATTCAG
Protein sequence

>MS.gene72195.t1

MASNLIKTSSLVLAALVALTVQVFYFSPIDPVILEIPSAASSTSSSKNNQLQKVIKLGEGFLKQPEDVCVDKDGVLYTATRDGWIKRMVRNGNWENWKHIDSSSLLGITTSKDGGLIVCDATKGLLKVTEEDGFSVILSQVNGSQLMFADDVIEASDGNIYFSVPSTKFGMHNWYLDVLEARPHGQLLRYNPMSNETVIVHDDLAFANGVALSKDEDYLLVCETWKFRCLKHWLKGINKGKTNIFIENLPAGPDNINLAPDGSFWIALIQ