Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72205.t1 | AFK35673.1 | 81.8 | 77 | 2 | 1 | 1 | 65 | 16 | 92 | 1.80E-24 | 122.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72205.t1 | Q43207 | 66.7 | 75 | 12 | 2 | 3 | 65 | 21 | 94 | 2.3e-17 | 89.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72205.t1 | G7LF61 | 81.8 | 77 | 2 | 1 | 1 | 65 | 16 | 92 | 1.3e-24 | 122.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049262 | MS.gene72205 | 0.819878 | 9.22E-53 | -1.69E-46 |
| MS.gene049587 | MS.gene72205 | 0.807009 | 6.23E-50 | -1.69E-46 |
| MS.gene049853 | MS.gene72205 | 0.808351 | 3.23E-50 | -1.69E-46 |
| MS.gene050611 | MS.gene72205 | 0.827742 | 1.32E-54 | -1.69E-46 |
| MS.gene058515 | MS.gene72205 | 0.806792 | 6.93E-50 | -1.69E-46 |
| MS.gene059276 | MS.gene72205 | 0.817641 | 2.97E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 29 sgRNAs with CRISPR-Local
Find 53 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTATGTGTAGTTCATTATAC+TGG | 0.313269 | 5.2:-62023073 | MS.gene72205:intron |
| ACTACTTTCAATTTCACTCT+TGG | 0.361943 | 5.2:-62023004 | MS.gene72205:CDS |
| AGTTTGATTCTAGCAGAGAT+AGG | 0.388381 | 5.2:-62023030 | MS.gene72205:CDS |
| TCCCACATCGTACTATTATC+AGG | 0.413728 | 5.2:-62022431 | MS.gene72205:CDS |
| TATACTGGAACATTGCTTGA+TGG | 0.417512 | 5.2:-62023058 | MS.gene72205:CDS |
| CTTCAAGAATAATAATAGAT+AGG | 0.425201 | 5.2:+62022807 | None:intergenic |
| AAGAAGAAGCTTCTCAAAGA+AGG | 0.467998 | 5.2:-62023191 | MS.gene72205:CDS |
| GTTTGATTCTAGCAGAGATA+GGG | 0.475863 | 5.2:-62023029 | MS.gene72205:CDS |
| TAGGCAGATGGAAGGTGATA+AGG | 0.476180 | 5.2:+62022826 | None:intergenic |
| ATCTATTATTATTCTTGAAG+AGG | 0.490271 | 5.2:-62022804 | MS.gene72205:intron |
| GACTTCGACTTCGCCGGTGC+CGG | 0.516399 | 5.2:-62023269 | MS.gene72205:CDS |
| AATGAAATTAAAAGCATGGC+AGG | 0.519602 | 5.2:-62022463 | MS.gene72205:CDS |
| AATTGGATTATCGCCGGCAC+CGG | 0.525961 | 5.2:+62023256 | None:intergenic |
| GTGGGTACTAGAGAATTCAT+AGG | 0.534544 | 5.2:-62022405 | MS.gene72205:CDS |
| TTCAATTTCACTCTTGGACA+AGG | 0.538952 | 5.2:-62022998 | MS.gene72205:intron |
| GTCAAATGAAATTAAAAGCA+TGG | 0.545516 | 5.2:-62022467 | MS.gene72205:CDS |
| AATAATAATAGATAGGCAGA+TGG | 0.568838 | 5.2:+62022814 | None:intergenic |
| CTTGAGAATTGGATTATCGC+CGG | 0.576084 | 5.2:+62023250 | None:intergenic |
| CATATGTAGACACTGTCATA+GGG | 0.583382 | 5.2:+62022374 | None:intergenic |
| TTTGATTCTAGCAGAGATAG+GGG | 0.593989 | 5.2:-62023028 | MS.gene72205:CDS |
| TCGTACTATTATCAGGCAAG+TGG | 0.597519 | 5.2:-62022424 | MS.gene72205:CDS |
| AAAGACGACTTCGACTTCGC+CGG | 0.605504 | 5.2:-62023275 | None:intergenic |
| AAGCTTCTCAAAGAAGGTGA+AGG | 0.613170 | 5.2:-62023185 | MS.gene72205:intron |
| CGGCGATAATCCAATTCTCA+AGG | 0.616014 | 5.2:-62023249 | MS.gene72205:CDS |
| TGCCTGATAATAGTACGATG+TGG | 0.623695 | 5.2:+62022429 | None:intergenic |
| ATAATAGATAGGCAGATGGA+AGG | 0.627317 | 5.2:+62022818 | None:intergenic |
| CGTACTATTATCAGGCAAGT+GGG | 0.636740 | 5.2:-62022423 | MS.gene72205:CDS |
| GCCTGATAATAGTACGATGT+GGG | 0.647015 | 5.2:+62022430 | None:intergenic |
| GATAATCCAATTCTCAAGGT+CGG | 0.697498 | 5.2:-62023245 | MS.gene72205:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTGATTTTGTTATTTTAATT+GGG | - | chr5.2:62022709-62022728 | MS.gene72205:intron | 10.0% |
| !!! | TTTGATTTTGTTATTTTAAT+TGG | - | chr5.2:62022708-62022727 | MS.gene72205:intron | 10.0% |
| !! | AAATCAAACATAGAAATTAA+CGG | + | chr5.2:62022736-62022755 | None:intergenic | 15.0% |
| !!! | TTAATTTCTATGTTTGATTT+AGG | - | chr5.2:62022736-62022755 | MS.gene72205:intron | 15.0% |
| !! | ATCTATTATTATTCTTGAAG+AGG | - | chr5.2:62022856-62022875 | MS.gene72205:intron | 20.0% |
| !! | CTTCAAGAATAATAATAGAT+AGG | + | chr5.2:62022856-62022875 | None:intergenic | 20.0% |
| !!! | TCTTTTATTTTCTTCACAAA+TGG | + | chr5.2:62023098-62023117 | None:intergenic | 20.0% |
| ! | AATAATAATAGATAGGCAGA+TGG | + | chr5.2:62022849-62022868 | None:intergenic | 25.0% |
| ! | GATTTCAAACAATAAAAAGC+AGG | - | chr5.2:62022946-62022965 | MS.gene72205:intron | 25.0% |
| ! | GTCAAATGAAATTAAAAGCA+TGG | - | chr5.2:62023193-62023212 | MS.gene72205:CDS | 25.0% |
| ! | TTATGTGTAGTTCATTATAC+TGG | - | chr5.2:62022587-62022606 | MS.gene72205:intron | 25.0% |
| !! | ACCTTTAATTTCCTCATAAT+AGG | + | chr5.2:62023039-62023058 | None:intergenic | 25.0% |
| !! | CCTTTAATTTCCTCATAATA+GGG | + | chr5.2:62023038-62023057 | None:intergenic | 25.0% |
| !! | TGTTTGATTTAGGTTATGTT+TGG | - | chr5.2:62022746-62022765 | MS.gene72205:intron | 25.0% |
| !!! | GAGGTAAATTTTTAAAAGTC+AGG | - | chr5.2:62022875-62022894 | MS.gene72205:intron | 25.0% |
| AATCAACGAAGAAAGAACAT+AGG | + | chr5.2:62022527-62022546 | None:intergenic | 30.0% | |
| ACTACTTTCAATTTCACTCT+TGG | - | chr5.2:62022656-62022675 | MS.gene72205:intron | 30.0% | |
| CCCTATTATGAGGAAATTAA+AGG | - | chr5.2:62023035-62023054 | MS.gene72205:CDS | 30.0% | |
| TAGTTATCAGCAAATGAGAT+AGG | - | chr5.2:62023010-62023029 | MS.gene72205:CDS | 30.0% | |
| ! | AATGAAATTAAAAGCATGGC+AGG | - | chr5.2:62023197-62023216 | MS.gene72205:CDS | 30.0% |
| ! | CATTTGCTGATAACTACAAA+AGG | + | chr5.2:62023006-62023025 | None:intergenic | 30.0% |
| !! | TGAAAAGTGCTTATCATATC+AGG | - | chr5.2:62022800-62022819 | MS.gene72205:intron | 30.0% |
| AAAAAGGAAATCGGAAAGCA+AGG | - | chr5.2:62022442-62022461 | MS.gene72205:CDS | 35.0% | |
| AAGAAGAAGCTTCTCAAAGA+AGG | - | chr5.2:62022469-62022488 | MS.gene72205:CDS | 35.0% | |
| AGTTTGATTCTAGCAGAGAT+AGG | - | chr5.2:62022630-62022649 | MS.gene72205:intron | 35.0% | |
| ATAATAGATAGGCAGATGGA+AGG | + | chr5.2:62022845-62022864 | None:intergenic | 35.0% | |
| GATAATCCAATTCTCAAGGT+CGG | - | chr5.2:62022415-62022434 | MS.gene72205:CDS | 35.0% | |
| GTCGGTGAAAAAAAGGAAAT+CGG | - | chr5.2:62022433-62022452 | MS.gene72205:CDS | 35.0% | |
| GTTTGATTCTAGCAGAGATA+GGG | - | chr5.2:62022631-62022650 | MS.gene72205:intron | 35.0% | |
| TATACTGGAACATTGCTTGA+TGG | - | chr5.2:62022602-62022621 | MS.gene72205:intron | 35.0% | |
| TTCAATTTCACTCTTGGACA+AGG | - | chr5.2:62022662-62022681 | MS.gene72205:intron | 35.0% | |
| TTTGATTCTAGCAGAGATAG+GGG | - | chr5.2:62022632-62022651 | MS.gene72205:intron | 35.0% | |
| !! | GCTGCTTACTTTTGATATTG+TGG | - | chr5.2:62023124-62023143 | MS.gene72205:intron | 35.0% |
| AAGCTTCTCAAAGAAGGTGA+AGG | - | chr5.2:62022475-62022494 | MS.gene72205:CDS | 40.0% | |
| CGTACTATTATCAGGCAAGT+GGG | - | chr5.2:62023237-62023256 | MS.gene72205:CDS | 40.0% | |
| CTTGAGAATTGGATTATCGC+CGG | + | chr5.2:62022413-62022432 | None:intergenic | 40.0% | |
| GAGATAGGATCCCTATTATG+AGG | - | chr5.2:62023025-62023044 | MS.gene72205:CDS | 40.0% | |
| GCCTGATAATAGTACGATGT+GGG | + | chr5.2:62023233-62023252 | None:intergenic | 40.0% | |
| GTGGGTACTAGAGAATTCAT+AGG | - | chr5.2:62023255-62023274 | MS.gene72205:CDS | 40.0% | |
| TCCCACATCGTACTATTATC+AGG | - | chr5.2:62023229-62023248 | MS.gene72205:CDS | 40.0% | |
| TCGTACTATTATCAGGCAAG+TGG | - | chr5.2:62023236-62023255 | MS.gene72205:CDS | 40.0% | |
| TCTCAAAGAAGGTGAAGGTT+GGG | - | chr5.2:62022480-62022499 | MS.gene72205:CDS | 40.0% | |
| TCTCAAGGTCGGTGAAAAAA+AGG | - | chr5.2:62022426-62022445 | MS.gene72205:CDS | 40.0% | |
| TGCCTGATAATAGTACGATG+TGG | + | chr5.2:62023234-62023253 | None:intergenic | 40.0% | |
| TTCTCAAAGAAGGTGAAGGT+TGG | - | chr5.2:62022479-62022498 | MS.gene72205:CDS | 40.0% | |
| ! | TTTTCACCGACCTTGAGAAT+TGG | + | chr5.2:62022424-62022443 | None:intergenic | 40.0% |
| AGGTTAGTGCATTGGAACGA+CGG | - | chr5.2:62022682-62022701 | MS.gene72205:intron | 45.0% | |
| CGGCGATAATCCAATTCTCA+AGG | - | chr5.2:62022411-62022430 | MS.gene72205:CDS | 45.0% | |
| CTTGGACAAGGTTAGTGCAT+TGG | - | chr5.2:62022674-62022693 | MS.gene72205:intron | 45.0% | |
| TAGGCAGATGGAAGGTGATA+AGG | + | chr5.2:62022837-62022856 | None:intergenic | 45.0% | |
| AATTGGATTATCGCCGGCAC+CGG | + | chr5.2:62022407-62022426 | None:intergenic | 50.0% | |
| GGTTGGGATGCTCATGATGT+TGG | - | chr5.2:62022496-62022515 | MS.gene72205:intron | 50.0% | |
| GACTTCGACTTCGCCGGTGC+CGG | - | chr5.2:62022391-62022410 | MS.gene72205:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.2 | gene | 62022389 | 62023293 | 62022389 | ID=MS.gene72205 |
| chr5.2 | mRNA | 62022389 | 62023293 | 62022389 | ID=MS.gene72205.t1;Parent=MS.gene72205 |
| chr5.2 | exon | 62023186 | 62023293 | 62023186 | ID=MS.gene72205.t1.exon1;Parent=MS.gene72205.t1 |
| chr5.2 | CDS | 62023186 | 62023293 | 62023186 | ID=cds.MS.gene72205.t1;Parent=MS.gene72205.t1 |
| chr5.2 | exon | 62022999 | 62023085 | 62022999 | ID=MS.gene72205.t1.exon2;Parent=MS.gene72205.t1 |
| chr5.2 | CDS | 62022999 | 62023085 | 62022999 | ID=cds.MS.gene72205.t1;Parent=MS.gene72205.t1 |
| chr5.2 | exon | 62022805 | 62022860 | 62022805 | ID=MS.gene72205.t1.exon3;Parent=MS.gene72205.t1 |
| chr5.2 | CDS | 62022805 | 62022860 | 62022805 | ID=cds.MS.gene72205.t1;Parent=MS.gene72205.t1 |
| chr5.2 | exon | 62022389 | 62022502 | 62022389 | ID=MS.gene72205.t1.exon4;Parent=MS.gene72205.t1 |
| chr5.2 | CDS | 62022389 | 62022502 | 62022389 | ID=cds.MS.gene72205.t1;Parent=MS.gene72205.t1 |
| Gene Sequence |
| Protein sequence |
