Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72610.t1 | RHN52323.1 | 84.9 | 159 | 23 | 1 | 22 | 179 | 170 | 328 | 2.20E-71 | 278.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72610.t1 | Q84JT7 | 47.4 | 95 | 46 | 1 | 53 | 143 | 362 | 456 | 5.8e-20 | 99.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72610.t1 | A0A396HIA3 | 84.9 | 159 | 23 | 1 | 22 | 179 | 170 | 328 | 1.6e-71 | 278.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050799 | MS.gene72610 | 0.810647 | 1.04E-50 | -1.69E-46 |
| MS.gene052190 | MS.gene72610 | 0.819804 | 9.58E-53 | -1.69E-46 |
| MS.gene052519 | MS.gene72610 | 0.82652 | 2.59E-54 | -1.69E-46 |
| MS.gene052863 | MS.gene72610 | 0.800802 | 1.21E-48 | -1.69E-46 |
| MS.gene053145 | MS.gene72610 | 0.812598 | 3.91E-51 | -1.69E-46 |
| MS.gene053900 | MS.gene72610 | 0.836413 | 9.50E-57 | -1.69E-46 |
| MS.gene054651 | MS.gene72610 | 0.807715 | 4.42E-50 | -1.69E-46 |
| MS.gene055818 | MS.gene72610 | 0.815732 | 7.95E-52 | -1.69E-46 |
| MS.gene058037 | MS.gene72610 | 0.801923 | 7.16E-49 | -1.69E-46 |
| MS.gene058625 | MS.gene72610 | 0.814042 | 1.88E-51 | -1.69E-46 |
| MS.gene060352 | MS.gene72610 | 0.813001 | 3.19E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72610.t1 | MTR_6g071350 | 85.401 | 137 | 19 | 1 | 44 | 179 | 265 | 401 | 6.51e-80 | 243 |
| MS.gene72610.t1 | MTR_5g048010 | 69.444 | 180 | 40 | 2 | 1 | 179 | 151 | 316 | 1.33e-69 | 214 |
| MS.gene72610.t1 | MTR_1g073380 | 63.576 | 151 | 41 | 3 | 20 | 156 | 351 | 501 | 4.46e-52 | 175 |
| MS.gene72610.t1 | MTR_1g073530 | 65.185 | 135 | 38 | 2 | 31 | 156 | 357 | 491 | 1.85e-50 | 171 |
| MS.gene72610.t1 | MTR_1g073590 | 64.444 | 135 | 39 | 2 | 31 | 156 | 354 | 488 | 6.72e-50 | 169 |
| MS.gene72610.t1 | MTR_8g098645 | 63.043 | 138 | 39 | 2 | 31 | 156 | 112 | 249 | 7.27e-49 | 161 |
| MS.gene72610.t1 | MTR_8g098645 | 68.293 | 41 | 13 | 0 | 139 | 179 | 306 | 346 | 2.23e-13 | 67.4 |
| MS.gene72610.t1 | MTR_8g098635 | 80.208 | 96 | 18 | 1 | 58 | 152 | 101 | 196 | 3.12e-47 | 156 |
| MS.gene72610.t1 | MTR_8g098635 | 73.684 | 38 | 10 | 0 | 142 | 179 | 260 | 297 | 5.70e-12 | 63.2 |
| MS.gene72610.t1 | MTR_8g098635 | 80.208 | 96 | 18 | 1 | 58 | 152 | 184 | 279 | 2.44e-46 | 156 |
| MS.gene72610.t1 | MTR_8g098635 | 73.684 | 38 | 10 | 0 | 142 | 179 | 343 | 380 | 9.48e-12 | 62.8 |
| MS.gene72610.t1 | MTR_8g098605 | 66.667 | 123 | 29 | 3 | 35 | 145 | 366 | 488 | 2.23e-41 | 146 |
| MS.gene72610.t1 | MTR_8g098605 | 70.732 | 41 | 12 | 0 | 139 | 179 | 556 | 596 | 5.76e-13 | 66.6 |
| MS.gene72610.t1 | MTR_8g028905 | 51.042 | 96 | 45 | 1 | 53 | 146 | 410 | 505 | 5.05e-25 | 101 |
| MS.gene72610.t1 | MTR_7g078120 | 52.632 | 95 | 44 | 1 | 53 | 146 | 389 | 483 | 6.27e-25 | 100 |
| MS.gene72610.t1 | MTR_7g078120 | 52.632 | 95 | 44 | 1 | 53 | 146 | 389 | 483 | 7.30e-25 | 100 |
| MS.gene72610.t1 | MTR_0259s0060 | 43.182 | 132 | 47 | 3 | 52 | 155 | 17 | 148 | 7.19e-24 | 92.4 |
| MS.gene72610.t1 | MTR_7g066890 | 37.374 | 99 | 55 | 3 | 53 | 145 | 196 | 293 | 1.53e-11 | 62.0 |
| MS.gene72610.t1 | MTR_7g066890 | 37.374 | 99 | 55 | 3 | 53 | 145 | 298 | 395 | 1.94e-11 | 62.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72610.t1 | AT2G46040 | 47.368 | 95 | 46 | 1 | 53 | 143 | 362 | 456 | 9.23e-24 | 97.4 |
| MS.gene72610.t1 | AT2G46040 | 47.368 | 95 | 46 | 1 | 53 | 143 | 362 | 456 | 9.23e-24 | 97.4 |
| MS.gene72610.t1 | AT2G46040 | 47.368 | 95 | 46 | 1 | 53 | 143 | 376 | 470 | 9.89e-24 | 97.4 |
| MS.gene72610.t1 | AT4G11400 | 44.792 | 96 | 50 | 1 | 53 | 145 | 376 | 471 | 2.86e-20 | 87.4 |
| MS.gene72610.t1 | AT4G11400 | 44.792 | 96 | 50 | 1 | 53 | 145 | 376 | 471 | 2.86e-20 | 87.4 |
| MS.gene72610.t1 | AT5G04110 | 33.566 | 143 | 82 | 5 | 27 | 156 | 334 | 476 | 5.91e-13 | 66.6 |
| MS.gene72610.t1 | AT5G04110 | 33.566 | 143 | 82 | 5 | 27 | 156 | 334 | 476 | 5.91e-13 | 66.6 |
| MS.gene72610.t1 | AT5G04110 | 33.566 | 143 | 82 | 5 | 27 | 156 | 335 | 477 | 6.57e-13 | 66.2 |
| MS.gene72610.t1 | AT5G04110 | 33.566 | 143 | 82 | 5 | 27 | 156 | 334 | 476 | 7.00e-13 | 66.2 |
Find 34 sgRNAs with CRISPR-Local
Find 60 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGTCAACTGCAGTGCTAATT+AGG | 0.136677 | 6.4:+48809670 | None:intergenic |
| CCTTTGGGGATTTGACAAAA+TGG | 0.297533 | 6.4:-48808930 | MS.gene72610:CDS |
| GAAGAAACTGAAGCTTGAAT+TGG | 0.300868 | 6.4:-48808966 | MS.gene72610:CDS |
| ACAAGTTCTGTTTCTGTAGT+AGG | 0.308191 | 6.4:+48809070 | None:intergenic |
| ATTAGGGCTACAAGGGTTAT+AGG | 0.320256 | 6.4:+48809687 | None:intergenic |
| ATAACAAATGGGTTGTGTTC+AGG | 0.333650 | 6.4:-48809715 | None:intergenic |
| GAAGTTAAGTTTGGATCATT+TGG | 0.345699 | 6.4:-48808902 | MS.gene72610:CDS |
| AATGCTTAGGCACACAAGTA+TGG | 0.365629 | 6.4:-48809111 | MS.gene72610:CDS |
| GCATCCATCTGTGGATTTCA+TGG | 0.379025 | 6.4:-48808852 | MS.gene72610:CDS |
| ATGGGCGAGGAAGTTAAGTT+TGG | 0.380276 | 6.4:-48808911 | MS.gene72610:CDS |
| GTCAACTGCAGTGCTAATTA+GGG | 0.401546 | 6.4:+48809671 | None:intergenic |
| CTTTGGGGATTTGACAAAAT+GGG | 0.434572 | 6.4:-48808929 | MS.gene72610:CDS |
| GATGTCAAATTGCTGCAAAC+AGG | 0.434612 | 6.4:-48808988 | MS.gene72610:CDS |
| CAGTGCTAATTAGGGCTACA+AGG | 0.444572 | 6.4:+48809679 | None:intergenic |
| CTACAGAAACAGAACTTGTT+GGG | 0.459894 | 6.4:-48809066 | MS.gene72610:CDS |
| AAGAAACTGAAGCTTGAATT+GGG | 0.460548 | 6.4:-48808965 | MS.gene72610:CDS |
| AAACTACACCAGTACATTGC+GGG | 0.464290 | 6.4:+48809149 | None:intergenic |
| ACTACAGAAACAGAACTTGT+TGG | 0.476498 | 6.4:-48809067 | MS.gene72610:CDS |
| GTGGAACTCCCGCAATGTAC+TGG | 0.476663 | 6.4:-48809157 | MS.gene72610:CDS |
| GAGTGTAGCTGCAACGTTCA+AGG | 0.494639 | 6.4:-48809028 | MS.gene72610:CDS |
| GAGAGTGACTCTAAATGCTT+AGG | 0.519975 | 6.4:-48809124 | MS.gene72610:CDS |
| AAGTTTGGATCATTTGGTGA+TGG | 0.523787 | 6.4:-48808896 | MS.gene72610:CDS |
| AATTAGCACTGCAGTTGACC+TGG | 0.533682 | 6.4:-48809667 | MS.gene72610:CDS |
| GTTGCAGGAAATAAAGTGCA+CGG | 0.596234 | 6.4:-48809268 | MS.gene72610:intron |
| CACTCCATGAAATCCACAGA+TGG | 0.598826 | 6.4:+48808848 | None:intergenic |
| GGGATTTGACAAAATGGGCG+AGG | 0.602480 | 6.4:-48808924 | MS.gene72610:CDS |
| TGAAAACGATGACCTACAGA+TGG | 0.625658 | 6.4:+48809181 | None:intergenic |
| CATTTGGTGATGGTTTCGAG+AGG | 0.630151 | 6.4:-48808886 | MS.gene72610:CDS |
| AGCTATCAAGCATCCATCTG+TGG | 0.633077 | 6.4:-48808861 | MS.gene72610:CDS |
| GAACATGTTAACGGATAACC+AGG | 0.641619 | 6.4:+48809649 | None:intergenic |
| ATTTGGTGATGGTTTCGAGA+GGG | 0.668136 | 6.4:-48808885 | MS.gene72610:CDS |
| AGTGCTAATTAGGGCTACAA+GGG | 0.673726 | 6.4:+48809680 | None:intergenic |
| TAAACTACACCAGTACATTG+CGG | 0.676923 | 6.4:+48809148 | None:intergenic |
| CTTGTTGGGAGAGAAAGACG+AGG | 0.678364 | 6.4:-48809052 | MS.gene72610:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTTTTGAAGAAAATATCA+TGG | + | chr6.4:48809090-48809109 | None:intergenic | 15.0% |
| ! | AAAAAATTTAAGCCATCTGT+AGG | - | chr6.4:48809329-48809348 | MS.gene72610:intron | 25.0% |
| AAATCATGCAGTTCAAATTC+AGG | + | chr6.4:48808915-48808934 | None:intergenic | 30.0% | |
| AAGAAACTGAAGCTTGAATT+GGG | - | chr6.4:48809557-48809576 | MS.gene72610:intron | 30.0% | |
| GAAGTTAAGTTTGGATCATT+TGG | - | chr6.4:48809620-48809639 | MS.gene72610:intron | 30.0% | |
| TAACATGTTCAACTTGAACT+GGG | - | chr6.4:48808886-48808905 | MS.gene72610:CDS | 30.0% | |
| TTAACATGTTCAACTTGAAC+TGG | - | chr6.4:48808885-48808904 | MS.gene72610:CDS | 30.0% | |
| ! | CTTTTTATTCACAACTGCTT+TGG | + | chr6.4:48809309-48809328 | None:intergenic | 30.0% |
| !! | GTTCAAGTTGAACATGTTAA+CGG | + | chr6.4:48808885-48808904 | None:intergenic | 30.0% |
| ACTACAGAAACAGAACTTGT+TGG | - | chr6.4:48809455-48809474 | MS.gene72610:intron | 35.0% | |
| ATCTTCTTCACCTTCTGTAA+AGG | + | chr6.4:48809134-48809153 | None:intergenic | 35.0% | |
| GAAGAAACTGAAGCTTGAAT+TGG | - | chr6.4:48809556-48809575 | MS.gene72610:intron | 35.0% | |
| TAAACTACACCAGTACATTG+CGG | + | chr6.4:48809377-48809396 | None:intergenic | 35.0% | |
| TGAAATTGAGATGCAGTGAA+AGG | - | chr6.4:48809183-48809202 | MS.gene72610:CDS | 35.0% | |
| TTGTAAGTGTCTCACTTTGA+CGG | + | chr6.4:48808952-48808971 | None:intergenic | 35.0% | |
| ! | AAGTTTGGATCATTTGGTGA+TGG | - | chr6.4:48809626-48809645 | MS.gene72610:intron | 35.0% |
| ! | CTACAGAAACAGAACTTGTT+GGG | - | chr6.4:48809456-48809475 | MS.gene72610:intron | 35.0% |
| ! | CTTTGGGGATTTGACAAAAT+GGG | - | chr6.4:48809593-48809612 | MS.gene72610:intron | 35.0% |
| !! | ACAAGTTCTGTTTCTGTAGT+AGG | + | chr6.4:48809455-48809474 | None:intergenic | 35.0% |
| !! | TTCTGAAACTCCTTTACAGA+AGG | - | chr6.4:48809121-48809140 | MS.gene72610:CDS | 35.0% |
| !!! | GTAGGTTTTGAATCATCAAC+AGG | + | chr6.4:48809437-48809456 | None:intergenic | 35.0% |
| AAACTACACCAGTACATTGC+GGG | + | chr6.4:48809376-48809395 | None:intergenic | 40.0% | |
| AATGCTTAGGCACACAAGTA+TGG | - | chr6.4:48809411-48809430 | MS.gene72610:intron | 40.0% | |
| AATTGAGATGCAGTGAAAGG+CGG | - | chr6.4:48809186-48809205 | MS.gene72610:CDS | 40.0% | |
| AGTGCTAATTAGGGCTACAA+GGG | + | chr6.4:48808845-48808864 | None:intergenic | 40.0% | |
| ATTAGGGCTACAAGGGTTAT+AGG | + | chr6.4:48808838-48808857 | None:intergenic | 40.0% | |
| CTGCATCTCAATTTCACTAC+TGG | + | chr6.4:48809179-48809198 | None:intergenic | 40.0% | |
| GAGAGTGACTCTAAATGCTT+AGG | - | chr6.4:48809398-48809417 | MS.gene72610:intron | 40.0% | |
| GATGTCAAATTGCTGCAAAC+AGG | - | chr6.4:48809534-48809553 | MS.gene72610:intron | 40.0% | |
| GTCAACTGCAGTGCTAATTA+GGG | + | chr6.4:48808854-48808873 | None:intergenic | 40.0% | |
| GTTGCAGGAAATAAAGTGCA+CGG | - | chr6.4:48809254-48809273 | MS.gene72610:CDS | 40.0% | |
| TGAAAACGATGACCTACAGA+TGG | + | chr6.4:48809344-48809363 | None:intergenic | 40.0% | |
| TGCATTTCACTTTCTGTTGC+AGG | - | chr6.4:48809239-48809258 | MS.gene72610:CDS | 40.0% | |
| ! | CCATTTTGTCAAATCCCCAA+AGG | + | chr6.4:48809595-48809614 | None:intergenic | 40.0% |
| ! | CCTTTGGGGATTTGACAAAA+TGG | - | chr6.4:48809592-48809611 | MS.gene72610:intron | 40.0% |
| ! | GAACATGTTAACGGATAACC+AGG | + | chr6.4:48808876-48808895 | None:intergenic | 40.0% |
| ! | GGGTTCTGCATTTTACCTTT+GGG | - | chr6.4:48809577-48809596 | MS.gene72610:intron | 40.0% |
| ! | GGTTCTGCATTTTACCTTTG+GGG | - | chr6.4:48809578-48809597 | MS.gene72610:intron | 40.0% |
| ! | TGGGTTCTGCATTTTACCTT+TGG | - | chr6.4:48809576-48809595 | MS.gene72610:intron | 40.0% |
| !! | ATTTGGTGATGGTTTCGAGA+GGG | - | chr6.4:48809637-48809656 | MS.gene72610:intron | 40.0% |
| !! | TGTAGGTCATCGTTTTCAAG+TGG | - | chr6.4:48809346-48809365 | MS.gene72610:intron | 40.0% |
| AATTAGCACTGCAGTTGACC+TGG | - | chr6.4:48808855-48808874 | MS.gene72610:CDS | 45.0% | |
| AGCTATCAAGCATCCATCTG+TGG | - | chr6.4:48809661-48809680 | MS.gene72610:CDS | 45.0% | |
| ATGGGCGAGGAAGTTAAGTT+TGG | - | chr6.4:48809611-48809630 | MS.gene72610:intron | 45.0% | |
| CACTACTGGATCCTCATACA+TGG | + | chr6.4:48809165-48809184 | None:intergenic | 45.0% | |
| CACTCCATGAAATCCACAGA+TGG | + | chr6.4:48809677-48809696 | None:intergenic | 45.0% | |
| CAGTGCTAATTAGGGCTACA+AGG | + | chr6.4:48808846-48808865 | None:intergenic | 45.0% | |
| GCATCCATCTGTGGATTTCA+TGG | - | chr6.4:48809670-48809689 | MS.gene72610:CDS | 45.0% | |
| GGTCAACTGCAGTGCTAATT+AGG | + | chr6.4:48808855-48808874 | None:intergenic | 45.0% | |
| !! | AAGAACAGCGCGGTTTTGTA+GGG | + | chr6.4:48809217-48809236 | None:intergenic | 45.0% |
| !! | CATTTGGTGATGGTTTCGAG+AGG | - | chr6.4:48809636-48809655 | MS.gene72610:intron | 45.0% |
| ACTGGATCCTCATACATGGC+AGG | + | chr6.4:48809161-48809180 | None:intergenic | 50.0% | |
| CTTGTTGGGAGAGAAAGACG+AGG | - | chr6.4:48809470-48809489 | MS.gene72610:intron | 50.0% | |
| GAAATGCAGCAAGAACAGCG+CGG | + | chr6.4:48809227-48809246 | None:intergenic | 50.0% | |
| GAGTGTAGCTGCAACGTTCA+AGG | - | chr6.4:48809494-48809513 | MS.gene72610:intron | 50.0% | |
| GATGCATCCTGCCATGTATG+AGG | - | chr6.4:48809151-48809170 | MS.gene72610:CDS | 50.0% | |
| GGGATTTGACAAAATGGGCG+AGG | - | chr6.4:48809598-48809617 | MS.gene72610:intron | 50.0% | |
| !! | CAAGAACAGCGCGGTTTTGT+AGG | + | chr6.4:48809218-48809237 | None:intergenic | 50.0% |
| GTGGAACTCCCGCAATGTAC+TGG | - | chr6.4:48809365-48809384 | MS.gene72610:intron | 55.0% | |
| !! | CAGCGCGGTTTTGTAGGGAC+AGG | + | chr6.4:48809212-48809231 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.4 | gene | 48808814 | 48809730 | 48808814 | ID=MS.gene72610 |
| chr6.4 | mRNA | 48808814 | 48809730 | 48808814 | ID=MS.gene72610.t1;Parent=MS.gene72610 |
| chr6.4 | exon | 48809661 | 48809730 | 48809661 | ID=MS.gene72610.t1.exon1;Parent=MS.gene72610.t1 |
| chr6.4 | CDS | 48809661 | 48809730 | 48809661 | ID=cds.MS.gene72610.t1;Parent=MS.gene72610.t1 |
| chr6.4 | exon | 48808814 | 48809283 | 48808814 | ID=MS.gene72610.t1.exon2;Parent=MS.gene72610.t1 |
| chr6.4 | CDS | 48808814 | 48809283 | 48808814 | ID=cds.MS.gene72610.t1;Parent=MS.gene72610.t1 |
| Gene Sequence |
| Protein sequence |
