AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene053145


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene053145.t1 MTR_4g008180 88.235 119 14 0 8 126 56 174 3.78e-72 213
MS.gene053145.t1 MTR_1g116085 52.688 93 33 2 8 97 17 101 3.05e-25 92.0
MS.gene053145.t1 MTR_4g007210 44.068 118 38 2 8 125 237 326 8.12e-22 89.0
MS.gene053145.t1 MTR_8g060700 52.113 71 31 1 9 76 669 739 3.50e-16 73.6
MS.gene053145.t1 MTR_1g092460 35.833 120 32 3 8 124 343 420 1.82e-13 65.9
MS.gene053145.t1 MTR_1g092440 48.387 62 29 1 8 66 547 608 3.41e-13 65.1
MS.gene053145.t1 MTR_0274s0030 41.026 78 43 1 1 75 273 350 2.56e-12 62.4
MS.gene053145.t1 MTR_2g079270 33.884 121 35 3 8 125 473 551 1.03e-11 60.8
MS.gene053145.t1 MTR_2g079280 51.786 56 24 1 1 53 255 310 1.78e-11 60.1
MS.gene053145.t1 MTR_2g018540 46.575 73 36 1 8 77 275 347 6.52e-11 58.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene053145.t1 AT1G53025 42.254 71 38 1 8 75 440 510 5.36e-11 58.9
MS.gene053145.t1 AT1G53025 42.254 71 38 1 8 75 440 510 5.38e-11 58.9

Find 30 sgRNAs with CRISPR-Local

Find 45 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTTTAAATCTTTATGTTTC+AGG 0.144505 4.2:-91654394 None:intergenic
CTCCAGTTCCTGTAAAATTT+AGG 0.153751 4.2:+91653841 None:intergenic
GTCAAAGAGTTTATAAGAAT+TGG 0.253351 4.2:-91653717 MS.gene053145:CDS
TCCAGTTCCTGTAAAATTTA+GGG 0.279949 4.2:+91653842 None:intergenic
AAAATGAGGTGTTTGGTTAA+AGG 0.326826 4.2:-91653939 MS.gene053145:CDS
GAGGGTGGCGAAAGCTGCTC+GGG 0.357354 4.2:-91653906 MS.gene053145:CDS
TTTGGTTAAAGGTGCGGTTC+AGG 0.382438 4.2:-91653928 MS.gene053145:CDS
CGTTTCTTGTTGCTCAATCA+AGG 0.383537 4.2:+91653673 None:intergenic
AGTGATTTATAATAGTTTGT+TGG 0.399724 4.2:-91653760 MS.gene053145:CDS
ATCTTTATGTTTCAGGATTG+TGG 0.420666 4.2:-91654387 None:intergenic
GGAACTGGAGCTGTGTACTC+TGG 0.427956 4.2:-91653828 MS.gene053145:CDS
GGAGGGTGGCGAAAGCTGCT+CGG 0.443819 4.2:-91653907 MS.gene053145:CDS
GAACTGGAGCTGTGTACTCT+GGG 0.467680 4.2:-91653827 MS.gene053145:CDS
GACAAAAGCAAGAGATCAAA+CGG 0.506852 4.2:-91653787 MS.gene053145:CDS
CAGTCGTGCTCATGATATCA+TGG 0.515430 4.2:-91653991 MS.gene053145:CDS
TTTAGCTAGAAACATGAAAG+TGG 0.518708 4.2:-91653868 MS.gene053145:CDS
GGTTAAAGGTGCGGTTCAGG+AGG 0.522848 4.2:-91653925 MS.gene053145:CDS
TATGTTTCAGGATTGTGGCA+TGG 0.529360 4.2:-91654382 MS.gene053145:intron
AGGTGCTAAAATGAGGTGTT+TGG 0.540651 4.2:-91653946 MS.gene053145:CDS
GGTGGCATGCAATGCATACA+TGG 0.545214 4.2:-91653970 MS.gene053145:CDS
AAAGGTGCGGTTCAGGAGGG+TGG 0.560811 4.2:-91653921 MS.gene053145:CDS
GTTAAAGGTGCGGTTCAGGA+GGG 0.572705 4.2:-91653924 MS.gene053145:CDS
ACATGGAAGGTGCTAAAATG+AGG 0.582197 4.2:-91653953 MS.gene053145:CDS
AGGGTGGCGAAAGCTGCTCG+GGG 0.616613 4.2:-91653905 MS.gene053145:CDS
GAGGTGTTTGGTTAAAGGTG+CGG 0.636534 4.2:-91653934 MS.gene053145:CDS
AAGGAATTTCTCGCAGCACG+TGG 0.659585 4.2:+91653692 None:intergenic
AACTGGAGCTGTGTACTCTG+GGG 0.660138 4.2:-91653826 MS.gene053145:CDS
GCATGCAATGCATACATGGA+AGG 0.663626 4.2:-91653966 MS.gene053145:CDS
ACTGGAGCTGTGTACTCTGG+GGG 0.665298 4.2:-91653825 MS.gene053145:CDS
TCGTGCTCATGATATCATGG+TGG 0.752987 4.2:-91653988 MS.gene053145:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGTATTTATTAGTAAAGTAA+TGG + chr4.2:91653732-91653751 None:intergenic 15.0%
!! TTGTTGAATTTATGTATATA+TGG - chr4.2:91653753-91653772 MS.gene053145:CDS 15.0%
!!! AGTGATTTATAATAGTTTGT+TGG - chr4.2:91654307-91654326 MS.gene053145:intron 20.0%
!!! TTATTAGTAAAGTAATGGAA+AGG + chr4.2:91653727-91653746 None:intergenic 20.0%
! AATGTTACCTTTCAAATTGT+TGG - chr4.2:91653981-91654000 MS.gene053145:CDS 25.0%
! CAATATACTGATATATAGAG+AGG - chr4.2:91653910-91653929 MS.gene053145:CDS 25.0%
! CTATAAACCAACAATTTGAA+AGG + chr4.2:91653991-91654010 None:intergenic 25.0%
! GTCAAAGAGTTTATAAGAAT+TGG - chr4.2:91654350-91654369 MS.gene053145:intron 25.0%
!!! TCAAATTGTTGGTTTATAGA+TGG - chr4.2:91653992-91654011 MS.gene053145:CDS 25.0%
ATCTCCTATCAACAAAACAT+TGG + chr4.2:91653830-91653849 None:intergenic 30.0%
TCCAGTTCCTGTAAAATTTA+GGG + chr4.2:91654228-91654247 None:intergenic 30.0%
TTTAGCTAGAAACATGAAAG+TGG - chr4.2:91654199-91654218 MS.gene053145:intron 30.0%
! AAAATGAGGTGTTTGGTTAA+AGG - chr4.2:91654128-91654147 MS.gene053145:intron 30.0%
!! GTAACATTTGTTATGTAGCA+TGG + chr4.2:91653969-91653988 None:intergenic 30.0%
AAAGCACAAACAAACCAATC+AGG + chr4.2:91653794-91653813 None:intergenic 35.0%
AAGCACAAACAAACCAATCA+GGG + chr4.2:91653793-91653812 None:intergenic 35.0%
CTCCAGTTCCTGTAAAATTT+AGG + chr4.2:91654229-91654248 None:intergenic 35.0%
GACAAAAGCAAGAGATCAAA+CGG - chr4.2:91654280-91654299 MS.gene053145:intron 35.0%
GTAGATAGTATATCCCTGAT+TGG - chr4.2:91653777-91653796 MS.gene053145:CDS 35.0%
!! GCAACCAATGTTTTGTTGAT+AGG - chr4.2:91653823-91653842 MS.gene053145:CDS 35.0%
!! TCTCTTGCTTTTGTCATCAT+AGG + chr4.2:91654275-91654294 None:intergenic 35.0%
! AGGTGCTAAAATGAGGTGTT+TGG - chr4.2:91654121-91654140 MS.gene053145:intron 40.0%
! GCCCTAAATTTTACAGGAAC+TGG - chr4.2:91654224-91654243 MS.gene053145:intron 40.0%
!! ACATGGAAGGTGCTAAAATG+AGG - chr4.2:91654114-91654133 MS.gene053145:intron 40.0%
CAGTCGTGCTCATGATATCA+TGG - chr4.2:91654076-91654095 MS.gene053145:intron 45.0%
CGACGAGGTCAACTTAATCA+AGG + chr4.2:91653943-91653962 None:intergenic 45.0%
GCATGCAATGCATACATGGA+AGG - chr4.2:91654101-91654120 MS.gene053145:intron 45.0%
TCGTGCTCATGATATCATGG+TGG - chr4.2:91654079-91654098 MS.gene053145:intron 45.0%
! GAGGTGTTTGGTTAAAGGTG+CGG - chr4.2:91654133-91654152 MS.gene053145:intron 45.0%
! GTGGCTGCCCTAAATTTTAC+AGG - chr4.2:91654218-91654237 MS.gene053145:intron 45.0%
! TTTGGTTAAAGGTGCGGTTC+AGG - chr4.2:91654139-91654158 MS.gene053145:intron 45.0%
!! TGATTAAGTTGACCTCGTCG+TGG - chr4.2:91653943-91653962 MS.gene053145:CDS 45.0%
AACTGGAGCTGTGTACTCTG+GGG - chr4.2:91654241-91654260 MS.gene053145:intron 50.0%
AAGGAATTTCTCGCAGCACG+TGG + chr4.2:91654378-91654397 None:intergenic 50.0%
GAACTGGAGCTGTGTACTCT+GGG - chr4.2:91654240-91654259 MS.gene053145:intron 50.0%
GGTGGCATGCAATGCATACA+TGG - chr4.2:91654097-91654116 MS.gene053145:intron 50.0%
! GTTAAAGGTGCGGTTCAGGA+GGG - chr4.2:91654143-91654162 MS.gene053145:intron 50.0%
ACTGGAGCTGTGTACTCTGG+GGG - chr4.2:91654242-91654261 MS.gene053145:intron 55.0%
GGAACTGGAGCTGTGTACTC+TGG - chr4.2:91654239-91654258 MS.gene053145:intron 55.0%
TATGTAGCATGGCCACGACG+AGG + chr4.2:91653958-91653977 None:intergenic 55.0%
! GGTTAAAGGTGCGGTTCAGG+AGG - chr4.2:91654142-91654161 MS.gene053145:intron 55.0%
! AAAGGTGCGGTTCAGGAGGG+TGG - chr4.2:91654146-91654165 MS.gene053145:intron 60.0%
AGGGTGGCGAAAGCTGCTCG+GGG - chr4.2:91654162-91654181 MS.gene053145:intron 65.0%
GAGGGTGGCGAAAGCTGCTC+GGG - chr4.2:91654161-91654180 MS.gene053145:intron 65.0%
GGAGGGTGGCGAAAGCTGCT+CGG - chr4.2:91654160-91654179 MS.gene053145:intron 65.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 91653686 91654403 91653686 ID=MS.gene053145
chr4.2 mRNA 91653686 91654403 91653686 ID=MS.gene053145.t1;Parent=MS.gene053145
chr4.2 exon 91654383 91654403 91654383 ID=MS.gene053145.t1.exon1;Parent=MS.gene053145.t1
chr4.2 CDS 91654383 91654403 91654383 ID=cds.MS.gene053145.t1;Parent=MS.gene053145.t1
chr4.2 exon 91653686 91654045 91653686 ID=MS.gene053145.t1.exon2;Parent=MS.gene053145.t1
chr4.2 CDS 91653686 91654045 91653686 ID=cds.MS.gene053145.t1;Parent=MS.gene053145.t1
Gene Sequence

>MS.gene053145

ATGTTTCAGGATTGTGGCATGGTTAGTTTTCGATTTTTCCTTTCCATTACTTTACTAATAAATACACTTGTTGAATTTATGTATATATGGAGTAGATAGTATATCCCTGATTGGTTTGTTTGTGCTTTGTAATTTGAGCAACCAATGTTTTGTTGATAGGAGATGTGCACTTCTTTTCTCGAGCAACCAATGTTTTGTTGATAGGAGATGTACACTTCTTTTCTCAATATACTGATATATAGAGAGGTAACTAACCTTGATTAAGTTGACCTCGTCGTGGCCATGCTACATAACAAATGTTACCTTTCAAATTGTTGGTTTATAGATGGCAAAGTTAATTTTTTATTTTCTTTTTCAGCATTTTGAAGACTTTGTTGTGAGACATTTTATCAGTCGTGCTCATGATATCATGGTGGCATGCAATGCATACATGGAAGGTGCTAAAATGAGGTGTTTGGTTAAAGGTGCGGTTCAGGAGGGTGGCGAAAGCTGCTCGGGGCAGTCTAAGACTTCTTTAGCTAGAAACATGAAAGTGGCTGCCCTAAATTTTACAGGAACTGGAGCTGTGTACTCTGGGGGTATCATTCCTATGATGACAAAAGCAAGAGATCAAACGGTCTCAGTGATTTATAATAGTTTGTTGGCTGAACGTATGAAAACGATTGTCAAAGAGTTTATAAGAATTGGAGCCACGTGCTGCGAGAAATTCCTTGATTGA

Protein sequence

>MS.gene053145.t1

MFQDCGMHFEDFVVRHFISRAHDIMVACNAYMEGAKMRCLVKGAVQEGGESCSGQSKTSLARNMKVAALNFTGTGAVYSGGIIPMMTKARDQTVSVIYNSLLAERMKTIVKEFIRIGATCCEKFLD