Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73333.t1 | XP_003594956.1 | 95.5 | 179 | 8 | 0 | 1 | 179 | 1 | 179 | 1.30E-87 | 332.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73333.t1 | Q9SUM5 | 34.1 | 173 | 92 | 4 | 7 | 179 | 8 | 158 | 5.0e-16 | 85.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73333.t1 | G7IN04 | 95.5 | 179 | 8 | 0 | 1 | 179 | 1 | 179 | 9.3e-88 | 332.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050226 | MS.gene73333 | 0.819372 | 1.20E-52 | -1.69E-46 |
| MS.gene050227 | MS.gene73333 | 0.81816 | 2.27E-52 | -1.69E-46 |
| MS.gene050228 | MS.gene73333 | 0.823325 | 1.47E-53 | -1.69E-46 |
| MS.gene050586 | MS.gene73333 | 0.815354 | 9.65E-52 | -1.69E-46 |
| MS.gene050763 | MS.gene73333 | 0.802132 | 6.49E-49 | -1.69E-46 |
| MS.gene052665 | MS.gene73333 | 0.801719 | 7.89E-49 | -1.69E-46 |
| MS.gene053836 | MS.gene73333 | 0.820035 | 8.48E-53 | -1.69E-46 |
| MS.gene054379 | MS.gene73333 | 0.801084 | 1.06E-48 | -1.69E-46 |
| MS.gene056875 | MS.gene73333 | 0.80289 | 4.53E-49 | -1.69E-46 |
| MS.gene058168 | MS.gene73333 | 0.838001 | 3.73E-57 | -1.69E-46 |
| MS.gene058182 | MS.gene73333 | 0.80294 | 4.42E-49 | -1.69E-46 |
| MS.gene059124 | MS.gene73333 | 0.80307 | 4.15E-49 | -1.69E-46 |
| MS.gene060404 | MS.gene73333 | 0.996137 | 7.83E-224 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73333.t1 | MTR_2g036670 | 95.531 | 179 | 8 | 0 | 1 | 179 | 1 | 179 | 4.88e-126 | 352 |
| MS.gene73333.t1 | MTR_4g127700 | 47.024 | 168 | 60 | 6 | 2 | 169 | 3 | 141 | 9.61e-42 | 138 |
| MS.gene73333.t1 | MTR_2g083470 | 52.174 | 69 | 31 | 1 | 20 | 86 | 6 | 74 | 3.24e-14 | 66.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73333.t1 | AT2G18969 | 34.857 | 175 | 98 | 4 | 7 | 176 | 8 | 171 | 1.06e-26 | 100 |
| MS.gene73333.t1 | AT4G30180 | 34.091 | 176 | 88 | 4 | 7 | 179 | 8 | 158 | 1.36e-20 | 83.6 |
Find 24 sgRNAs with CRISPR-Local
Find 31 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATCTTATTAGCCTCAGATTT+TGG | 0.172199 | 2.4:+52797477 | None:intergenic |
| ATCTGAGGCTAATAAGATTT+TGG | 0.186879 | 2.4:-52797472 | MS.gene73333:CDS |
| GACAATACTTTCAACATTCT+TGG | 0.219614 | 2.4:+52797659 | None:intergenic |
| TTTGCTTGCACTACTTTGTT+TGG | 0.314099 | 2.4:+52797756 | None:intergenic |
| TCCTTGATCTTCATCAAAGC+TGG | 0.404897 | 2.4:+52797702 | None:intergenic |
| TTGGTTGAAGAAAATATTGA+TGG | 0.430271 | 2.4:-52797453 | MS.gene73333:CDS |
| GATGGTATGTGATGAGGAAA+TGG | 0.466333 | 2.4:-52797364 | MS.gene73333:CDS |
| CTTCAAAAGGATGGTGATGA+AGG | 0.470664 | 2.4:-52797564 | MS.gene73333:CDS |
| TAATTATCAACAAAATGAAA+TGG | 0.471422 | 2.4:-52797529 | MS.gene73333:CDS |
| TGTAAGTTGTTTGCAAATGC+AGG | 0.519711 | 2.4:-52797322 | MS.gene73333:CDS |
| CATACCATCTCTTCTCCTCC+TGG | 0.531281 | 2.4:+52797378 | None:intergenic |
| AGAAGAGATGGTATGTGATG+AGG | 0.532629 | 2.4:-52797370 | MS.gene73333:CDS |
| ACCAGCTTTGATGAAGATCA+AGG | 0.546207 | 2.4:-52797703 | MS.gene73333:CDS |
| AAAATGAAGCTTCAAAAGGA+TGG | 0.553863 | 2.4:-52797573 | MS.gene73333:CDS |
| TGGTGATGAAGATGAGATTG+TGG | 0.568954 | 2.4:-52797433 | MS.gene73333:CDS |
| AAGTATTGTCAAGTATGAAG+TGG | 0.579515 | 2.4:-52797646 | MS.gene73333:CDS |
| GTATGAAGTGGACATGGCAA+TGG | 0.582971 | 2.4:-52797634 | MS.gene73333:CDS |
| GATACCAGGAGGAGAAGAGA+TGG | 0.597097 | 2.4:-52797382 | MS.gene73333:CDS |
| TGTCTTAGAAAATTGATACC+AGG | 0.622054 | 2.4:-52797396 | MS.gene73333:CDS |
| TGTCAAGTATGAAGTGGACA+TGG | 0.630889 | 2.4:-52797640 | MS.gene73333:CDS |
| ATGGTGATGAAGGTTCATCA+AGG | 0.637436 | 2.4:-52797554 | MS.gene73333:CDS |
| TTCAACTTGTCTGATGAGTG+AGG | 0.643602 | 2.4:+52797283 | None:intergenic |
| AGATTGTGGATGACCAATTG+AGG | 0.657789 | 2.4:-52797419 | MS.gene73333:CDS |
| CTTAGAAAATTGATACCAGG+AGG | 0.680984 | 2.4:-52797393 | MS.gene73333:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CTTGATTTCTATAAAAAAAA+TGG | - | chr2.4:52797584-52797603 | MS.gene73333:CDS | 15.0% |
| !! | TAATTATCAACAAAATGAAA+TGG | - | chr2.4:52797556-52797575 | MS.gene73333:CDS | 15.0% |
| !!! | TTTTTTTATAGAAATCAAGT+GGG | + | chr2.4:52797583-52797602 | None:intergenic | 15.0% |
| !!! | TTTTTTTTATAGAAATCAAG+TGG | + | chr2.4:52797584-52797603 | None:intergenic | 15.0% |
| ! | TCTAAAAATGAAGCTTCAAA+AGG | - | chr2.4:52797508-52797527 | MS.gene73333:CDS | 25.0% |
| ! | TTGGTTGAAGAAAATATTGA+TGG | - | chr2.4:52797632-52797651 | MS.gene73333:CDS | 25.0% |
| AAAAAATGGTCCAAAATCTG+AGG | - | chr2.4:52797598-52797617 | MS.gene73333:CDS | 30.0% | |
| AAAATGAAGCTTCAAAAGGA+TGG | - | chr2.4:52797512-52797531 | MS.gene73333:CDS | 30.0% | |
| GACAATACTTTCAACATTCT+TGG | + | chr2.4:52797429-52797448 | None:intergenic | 30.0% | |
| TGTCTTAGAAAATTGATACC+AGG | - | chr2.4:52797689-52797708 | MS.gene73333:CDS | 30.0% | |
| ! | AAGTATTGTCAAGTATGAAG+TGG | - | chr2.4:52797439-52797458 | MS.gene73333:CDS | 30.0% |
| !! | ATCTGAGGCTAATAAGATTT+TGG | - | chr2.4:52797613-52797632 | MS.gene73333:CDS | 30.0% |
| !! | ATCTTATTAGCCTCAGATTT+TGG | + | chr2.4:52797611-52797630 | None:intergenic | 30.0% |
| !!! | ATTTTCTAAGACACCTCAAT+TGG | + | chr2.4:52797682-52797701 | None:intergenic | 30.0% |
| CTTAGAAAATTGATACCAGG+AGG | - | chr2.4:52797692-52797711 | MS.gene73333:CDS | 35.0% | |
| TGTAAGTTGTTTGCAAATGC+AGG | - | chr2.4:52797763-52797782 | MS.gene73333:CDS | 35.0% | |
| TTTGCTTGCACTACTTTGTT+TGG | + | chr2.4:52797332-52797351 | None:intergenic | 35.0% | |
| ! | TTTCATCTCAAGGTTTTGCA+TGG | - | chr2.4:52797477-52797496 | MS.gene73333:CDS | 35.0% |
| !!! | GCAATGGTTTTTTCATCTCA+AGG | - | chr2.4:52797467-52797486 | MS.gene73333:CDS | 35.0% |
| AGAAGAGATGGTATGTGATG+AGG | - | chr2.4:52797715-52797734 | MS.gene73333:CDS | 40.0% | |
| AGATTGTGGATGACCAATTG+AGG | - | chr2.4:52797666-52797685 | MS.gene73333:CDS | 40.0% | |
| GATGGTATGTGATGAGGAAA+TGG | - | chr2.4:52797721-52797740 | MS.gene73333:CDS | 40.0% | |
| TCCTTGATCTTCATCAAAGC+TGG | + | chr2.4:52797386-52797405 | None:intergenic | 40.0% | |
| TGTCAAGTATGAAGTGGACA+TGG | - | chr2.4:52797445-52797464 | MS.gene73333:CDS | 40.0% | |
| ! | ACCAGCTTTGATGAAGATCA+AGG | - | chr2.4:52797382-52797401 | MS.gene73333:CDS | 40.0% |
| ! | ATGGTGATGAAGGTTCATCA+AGG | - | chr2.4:52797531-52797550 | MS.gene73333:CDS | 40.0% |
| ! | CTTCAAAAGGATGGTGATGA+AGG | - | chr2.4:52797521-52797540 | MS.gene73333:CDS | 40.0% |
| ! | TGGTGATGAAGATGAGATTG+TGG | - | chr2.4:52797652-52797671 | MS.gene73333:CDS | 40.0% |
| GTATGAAGTGGACATGGCAA+TGG | - | chr2.4:52797451-52797470 | MS.gene73333:CDS | 45.0% | |
| CATACCATCTCTTCTCCTCC+TGG | + | chr2.4:52797710-52797729 | None:intergenic | 50.0% | |
| GATACCAGGAGGAGAAGAGA+TGG | - | chr2.4:52797703-52797722 | MS.gene73333:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.4 | gene | 52797284 | 52797823 | 52797284 | ID=MS.gene73333 |
| chr2.4 | mRNA | 52797284 | 52797823 | 52797284 | ID=MS.gene73333.t1;Parent=MS.gene73333 |
| chr2.4 | exon | 52797284 | 52797823 | 52797284 | ID=MS.gene73333.t1.exon1;Parent=MS.gene73333.t1 |
| chr2.4 | CDS | 52797284 | 52797823 | 52797284 | ID=cds.MS.gene73333.t1;Parent=MS.gene73333.t1 |
| Gene Sequence |
| Protein sequence |
