Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73434.t1 | AFK48706.1 | 93.6 | 173 | 7 | 1 | 23 | 195 | 48 | 216 | 1.20E-78 | 302.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73434.t1 | G8A288 | 93.6 | 173 | 7 | 1 | 23 | 195 | 48 | 216 | 8.6e-79 | 302.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049230 | MS.gene73434 | 0.808566 | 2.91E-50 | -1.69E-46 |
| MS.gene049378 | MS.gene73434 | 0.800622 | 1.32E-48 | -1.69E-46 |
| MS.gene050623 | MS.gene73434 | -0.816463 | 5.46E-52 | -1.69E-46 |
| MS.gene053381 | MS.gene73434 | 0.805186 | 1.51E-49 | -1.69E-46 |
| MS.gene058435 | MS.gene73434 | 0.809397 | 1.93E-50 | -1.69E-46 |
| MS.gene060084 | MS.gene73434 | 0.805543 | 1.27E-49 | -1.69E-46 |
| MS.gene060297 | MS.gene73434 | 0.817548 | 3.11E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73434.t1 | MTR_1g109370 | 93.642 | 173 | 7 | 1 | 23 | 195 | 48 | 216 | 1.40e-112 | 320 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73434.t1 | AT1G68185 | 46.452 | 155 | 81 | 2 | 42 | 195 | 62 | 215 | 4.32e-39 | 133 |
| MS.gene73434.t1 | AT1G68185 | 46.452 | 155 | 81 | 2 | 42 | 195 | 62 | 215 | 4.32e-39 | 133 |
| MS.gene73434.t1 | AT1G68185 | 46.452 | 155 | 81 | 2 | 42 | 195 | 108 | 261 | 1.87e-38 | 133 |
Find 45 sgRNAs with CRISPR-Local
Find 98 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCTCCTCATCAGTCTTCTTA+TGG | 0.228908 | 1.4:+83737141 | None:intergenic |
| ACCATCGAGGCACACGATAT+TGG | 0.263756 | 1.4:+83737537 | None:intergenic |
| AAAGCAGATTCGGATGTTTA+TGG | 0.264643 | 1.4:-83736455 | MS.gene73434:intron |
| CAAATTGTGTTGTCATTTGA+TGG | 0.307014 | 1.4:-83736128 | MS.gene73434:CDS |
| TATCATTATCTTCCATTTCA+AGG | 0.332434 | 1.4:+83736072 | None:intergenic |
| CACGGAAGGTTGCAATGAAT+TGG | 0.349044 | 1.4:+83736555 | None:intergenic |
| CCATCGAGGCACACGATATT+GGG | 0.373094 | 1.4:+83737538 | None:intergenic |
| AGTCTTCTTATGGGCCTTAC+CGG | 0.377421 | 1.4:+83737151 | None:intergenic |
| CTCCTCATCAGTCTTCTTAT+GGG | 0.387413 | 1.4:+83737142 | None:intergenic |
| AAACAAGGGTTCAAGTTCTT+CGG | 0.392297 | 1.4:+83737579 | None:intergenic |
| AACCCCTGCCAGCCTTGAAA+TGG | 0.394451 | 1.4:-83736084 | MS.gene73434:CDS |
| ATTCAACTTTGAAGAATTTG+AGG | 0.439470 | 1.4:-83737118 | MS.gene73434:intron |
| CCCAATATCGTGTGCCTCGA+TGG | 0.441844 | 1.4:-83737538 | MS.gene73434:intron |
| TTTGGGGTTTAGGTTGAGAA+TGG | 0.451301 | 1.4:-83737251 | MS.gene73434:intron |
| TCTTCCATTTCAAGGCTGGC+AGG | 0.456162 | 1.4:+83736080 | None:intergenic |
| AGGATGAAACAAAGCAGATT+CGG | 0.459454 | 1.4:-83736465 | MS.gene73434:CDS |
| TTCCATTTCAAGGCTGGCAG+GGG | 0.465493 | 1.4:+83736082 | None:intergenic |
| GCCTTACCGGTGACCCTTGG+TGG | 0.466237 | 1.4:+83737164 | None:intergenic |
| TGGGCCTTACCGGTGACCCT+TGG | 0.476360 | 1.4:+83737161 | None:intergenic |
| CAATTCATTGCAACCTTCCG+TGG | 0.476932 | 1.4:-83736554 | MS.gene73434:CDS |
| ATTATCTTCCATTTCAAGGC+TGG | 0.485099 | 1.4:+83736076 | None:intergenic |
| GCCACCAAGGGTCACCGGTA+AGG | 0.490738 | 1.4:-83737165 | MS.gene73434:CDS |
| CTTCCATTTCAAGGCTGGCA+GGG | 0.494291 | 1.4:+83736081 | None:intergenic |
| TTGGGGTTTAGGTTGAGAAT+GGG | 0.505216 | 1.4:-83737250 | MS.gene73434:intron |
| AGAATCTTTGCTCGTTCAGA+AGG | 0.520871 | 1.4:+83736505 | None:intergenic |
| TTTGTATAGATTGAAGAAGC+AGG | 0.529052 | 1.4:-83736650 | MS.gene73434:intron |
| TCACTTACGTCGTCCACGGA+AGG | 0.541348 | 1.4:+83736541 | None:intergenic |
| CCGGTGACCCTTGGTGGCGA+AGG | 0.548861 | 1.4:+83737170 | None:intergenic |
| TGTAGGATGACCAGTTTGAA+AGG | 0.550107 | 1.4:-83736193 | MS.gene73434:intron |
| CCTTCGCCACCAAGGGTCAC+CGG | 0.569098 | 1.4:-83737170 | MS.gene73434:CDS |
| TGAGAATGGGAAGAATACTA+AGG | 0.569816 | 1.4:-83737237 | MS.gene73434:CDS |
| TTGGTTACCTTCGCCACCAA+GGG | 0.579619 | 1.4:-83737177 | MS.gene73434:CDS |
| AACGCGACTGTAATCAAACA+AGG | 0.602179 | 1.4:+83737564 | None:intergenic |
| GAATGGGAAGAATACTAAGG+TGG | 0.613980 | 1.4:-83737234 | MS.gene73434:CDS |
| TGGACCTCGAGGATGATGAT+TGG | 0.623297 | 1.4:-83737196 | MS.gene73434:CDS |
| TCTCATCTCTGTTCAAGACA+AGG | 0.626807 | 1.4:-83736485 | MS.gene73434:CDS |
| CCATAAGAAGACTGATGAGG+AGG | 0.627181 | 1.4:-83737141 | MS.gene73434:CDS |
| ATTGGTTACCTTCGCCACCA+AGG | 0.629138 | 1.4:-83737178 | MS.gene73434:CDS |
| TAAGGTGGAAACAGTGAATG+TGG | 0.687527 | 1.4:-83737219 | MS.gene73434:CDS |
| GGCCCATAAGAAGACTGATG+AGG | 0.692280 | 1.4:-83737144 | MS.gene73434:CDS |
| AGTGAATGTGGTGGACCTCG+AGG | 0.707436 | 1.4:-83737207 | MS.gene73434:CDS |
| GGTGGAAACAGTGAATGTGG+TGG | 0.711849 | 1.4:-83737216 | MS.gene73434:CDS |
| TTAAGGAGTGCTAACCATCG+AGG | 0.734889 | 1.4:+83737524 | None:intergenic |
| ACGCGACTGTAATCAAACAA+GGG | 0.742183 | 1.4:+83737565 | None:intergenic |
| GTAACCAATCATCATCCTCG+AGG | 0.759157 | 1.4:+83737192 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAACAAAAAATTAAACATT+TGG | + | chr1.4:83736163-83736182 | None:intergenic | 10.0% |
| !! | AAACAAAAAATTAAACATTT+GGG | + | chr1.4:83736162-83736181 | None:intergenic | 10.0% |
| !! | TTAATTTCAATTTGAATAAT+CGG | + | chr1.4:83736135-83736154 | None:intergenic | 10.0% |
| !! | AACAAAAAATTAAACATTTG+GGG | + | chr1.4:83736161-83736180 | None:intergenic | 15.0% |
| !! | CTGATAAAGAAAATAAAAAA+AGG | + | chr1.4:83737332-83737351 | None:intergenic | 15.0% |
| !!! | GTAAATTTTGATAATGTTTT+GGG | - | chr1.4:83736360-83736379 | MS.gene73434:intron | 15.0% |
| !!! | TAAATTTTGATAATGTTTTG+GGG | - | chr1.4:83736361-83736380 | MS.gene73434:intron | 15.0% |
| !!! | TGTAAATTTTGATAATGTTT+TGG | - | chr1.4:83736359-83736378 | MS.gene73434:intron | 15.0% |
| !! | AATGGCAAAAAACAAAAATT+TGG | + | chr1.4:83736573-83736592 | None:intergenic | 20.0% |
| !! | ACAAAAAATTAAACATTTGG+GGG | + | chr1.4:83736160-83736179 | None:intergenic | 20.0% |
| !! | ATGAGTAGAATAACTTATAT+TGG | + | chr1.4:83736887-83736906 | None:intergenic | 20.0% |
| !! | CAAAACAAAAAACACAAATT+TGG | + | chr1.4:83736312-83736331 | None:intergenic | 20.0% |
| !! | TAAAATAACATGCTCATAAA+GGG | - | chr1.4:83736754-83736773 | MS.gene73434:intron | 20.0% |
| !! | TAAAGAAAATAAAAAAAGGC+AGG | + | chr1.4:83737328-83737347 | None:intergenic | 20.0% |
| !! | TTAAAATAACATGCTCATAA+AGG | - | chr1.4:83736753-83736772 | MS.gene73434:intron | 20.0% |
| !!! | TTGTATAAAAAAAGTGTGTT+GGG | - | chr1.4:83736802-83736821 | MS.gene73434:intron | 20.0% |
| ! | ACATCTTATAAGAAACTGTA+TGG | + | chr1.4:83736952-83736971 | None:intergenic | 25.0% |
| ! | TAACTAATTAAGCCTATTCT+TGG | - | chr1.4:83736849-83736868 | MS.gene73434:intron | 25.0% |
| ! | TAAGTAAACAAACAATGCAA+TGG | + | chr1.4:83736591-83736610 | None:intergenic | 25.0% |
| ! | TATCATTATCTTCCATTTCA+AGG | + | chr1.4:83737559-83737578 | None:intergenic | 25.0% |
| ! | TGTTGATGTTCTTAATATTG+TGG | - | chr1.4:83737227-83737246 | MS.gene73434:CDS | 25.0% |
| ! | TTGAATAATCGGAATAGTTA+AGG | + | chr1.4:83736124-83736143 | None:intergenic | 25.0% |
| !! | AGTTTGTTTGAAATGGTATA+AGG | - | chr1.4:83737285-83737304 | MS.gene73434:intron | 25.0% |
| !! | ATTCAACTTTGAAGAATTTG+AGG | - | chr1.4:83736510-83736529 | MS.gene73434:CDS | 25.0% |
| !! | CTTGTATAAAAAAAGTGTGT+TGG | - | chr1.4:83736801-83736820 | MS.gene73434:intron | 25.0% |
| !! | GTTTGTTTGAAATGGTATAA+GGG | - | chr1.4:83737286-83737305 | MS.gene73434:intron | 25.0% |
| !!! | TAGTTGTAGTTTGTTTGAAA+TGG | - | chr1.4:83737278-83737297 | MS.gene73434:intron | 25.0% |
| !!! | TGTAGCAATGTTTTAAAAAC+CGG | + | chr1.4:83736719-83736738 | None:intergenic | 25.0% |
| !!! | TGTGTTTTTTGTTTTGTACA+AGG | - | chr1.4:83736316-83736335 | MS.gene73434:intron | 25.0% |
| !!! | TTTTGATAATCCTTTCAAAC+TGG | + | chr1.4:83737448-83737467 | None:intergenic | 25.0% |
| AATTAAGCCTATTCTTGGTT+TGG | - | chr1.4:83736854-83736873 | MS.gene73434:intron | 30.0% | |
| ATCCTACAGAGAATTGAAAA+AGG | + | chr1.4:83737423-83737442 | None:intergenic | 30.0% | |
| GAATTCTTCTTTCTCTTCTT+AGG | + | chr1.4:83736258-83736277 | None:intergenic | 30.0% | |
| TAAAGTTCCAAACCAAGAAT+AGG | + | chr1.4:83736864-83736883 | None:intergenic | 30.0% | |
| TTTGTATAGATTGAAGAAGC+AGG | - | chr1.4:83736978-83736997 | MS.gene73434:intron | 30.0% | |
| TTTGTTTACTTAACACTCCT+AGG | - | chr1.4:83736599-83736618 | MS.gene73434:CDS | 30.0% | |
| ! | AACTTTGAAGAATTTGAGGT+TGG | - | chr1.4:83736514-83736533 | MS.gene73434:CDS | 30.0% |
| ! | CAAATTGTGTTGTCATTTGA+TGG | - | chr1.4:83737500-83737519 | MS.gene73434:intron | 30.0% |
| ! | TCAACATGTAGTGAGTATTT+TGG | - | chr1.4:83736284-83736303 | MS.gene73434:intron | 30.0% |
| !! | TATTTGTCACTTTATCTAGC+TGG | - | chr1.4:83737385-83737404 | MS.gene73434:intron | 30.0% |
| !! | TTGTTTTGTACAAGGATGTT+TGG | - | chr1.4:83736324-83736343 | MS.gene73434:intron | 30.0% |
| !! | TTTTCCGATTCGTTTGATTT+CGG | + | chr1.4:83736690-83736709 | None:intergenic | 30.0% |
| !!! | GTAGCAATGTTTTAAAAACC+GGG | + | chr1.4:83736718-83736737 | None:intergenic | 30.0% |
| !!! | TTGATAATGTTTTGGGGTTT+AGG | - | chr1.4:83736367-83736386 | MS.gene73434:intron | 30.0% |
| AAAGCAGATTCGGATGTTTA+TGG | - | chr1.4:83737173-83737192 | MS.gene73434:CDS | 35.0% | |
| ACAATGTAGTCTGATTGAAC+TGG | + | chr1.4:83736665-83736684 | None:intergenic | 35.0% | |
| AGGATGAAACAAAGCAGATT+CGG | - | chr1.4:83737163-83737182 | MS.gene73434:CDS | 35.0% | |
| ATTATCTTCCATTTCAAGGC+TGG | + | chr1.4:83737555-83737574 | None:intergenic | 35.0% | |
| GTAACCGAAATCAAACGAAT+CGG | - | chr1.4:83736683-83736702 | MS.gene73434:intron | 35.0% | |
| TATCAGAGCTGCATTTCTTT+AGG | - | chr1.4:83737346-83737365 | MS.gene73434:intron | 35.0% | |
| TGAGAATGGGAAGAATACTA+AGG | - | chr1.4:83736391-83736410 | MS.gene73434:intron | 35.0% | |
| ! | AAACAAGGGTTCAAGTTCTT+CGG | + | chr1.4:83736052-83736071 | None:intergenic | 35.0% |
| !! | CGGTTTAACTTGTTAAACCT+AGG | + | chr1.4:83736619-83736638 | None:intergenic | 35.0% |
| !!! | CTCCTTTTTCAATTCTCTGT+AGG | - | chr1.4:83737418-83737437 | MS.gene73434:intron | 35.0% |
| !!! | GGTTGTTTTTATTAGTGCTC+TGG | - | chr1.4:83737248-83737267 | MS.gene73434:intron | 35.0% |
| !!! | TTTTGCTGATTCAGCAAAAG+CGG | + | chr1.4:83737010-83737029 | None:intergenic | 35.0% |
| AACGCGACTGTAATCAAACA+AGG | + | chr1.4:83736067-83736086 | None:intergenic | 40.0% | |
| ACGCGACTGTAATCAAACAA+GGG | + | chr1.4:83736066-83736085 | None:intergenic | 40.0% | |
| AGAATCTTTGCTCGTTCAGA+AGG | + | chr1.4:83737126-83737145 | None:intergenic | 40.0% | |
| CTCCTCATCAGTCTTCTTAT+GGG | + | chr1.4:83736489-83736508 | None:intergenic | 40.0% | |
| GAATGGGAAGAATACTAAGG+TGG | - | chr1.4:83736394-83736413 | MS.gene73434:intron | 40.0% | |
| GAGTTGAATCAGTTGAATCG+CGG | + | chr1.4:83736643-83736662 | None:intergenic | 40.0% | |
| TAAGGTGGAAACAGTGAATG+TGG | - | chr1.4:83736409-83736428 | MS.gene73434:intron | 40.0% | |
| TCTCATCTCTGTTCAAGACA+AGG | - | chr1.4:83737143-83737162 | MS.gene73434:CDS | 40.0% | |
| TGTAGGATGACCAGTTTGAA+AGG | - | chr1.4:83737435-83737454 | MS.gene73434:intron | 40.0% | |
| ! | TTTTTCACTTACGTCGTCCA+CGG | + | chr1.4:83737094-83737113 | None:intergenic | 40.0% |
| !! | TTGGGGTTTAGGTTGAGAAT+GGG | - | chr1.4:83736378-83736397 | MS.gene73434:intron | 40.0% |
| !! | TTTGGGGTTTAGGTTGAGAA+TGG | - | chr1.4:83736377-83736396 | MS.gene73434:intron | 40.0% |
| ACGAATCGGAAAACTGAACC+CGG | - | chr1.4:83736697-83736716 | MS.gene73434:intron | 45.0% | |
| AGTCTTCTTATGGGCCTTAC+CGG | + | chr1.4:83736480-83736499 | None:intergenic | 45.0% | |
| CAATTCATTGCAACCTTCCG+TGG | - | chr1.4:83737074-83737093 | MS.gene73434:intron | 45.0% | |
| CACGGAAGGTTGCAATGAAT+TGG | + | chr1.4:83737076-83737095 | None:intergenic | 45.0% | |
| CCATAAGAAGACTGATGAGG+AGG | - | chr1.4:83736487-83736506 | MS.gene73434:CDS | 45.0% | |
| CCTCCTCATCAGTCTTCTTA+TGG | + | chr1.4:83736490-83736509 | None:intergenic | 45.0% | |
| GTAACCAATCATCATCCTCG+AGG | + | chr1.4:83736439-83736458 | None:intergenic | 45.0% | |
| TGAATCAGTTGAATCGCGGT+CGG | + | chr1.4:83736639-83736658 | None:intergenic | 45.0% | |
| !! | TTAAGGAGTGCTAACCATCG+AGG | + | chr1.4:83736107-83736126 | None:intergenic | 45.0% |
| !!! | TATTTTAGAATAAAAATAAA+AGG | + | chr1.4:83736186-83736205 | None:intergenic | 5.0% |
| ACCATCGAGGCACACGATAT+TGG | + | chr1.4:83736094-83736113 | None:intergenic | 50.0% | |
| AGGCAGGCAGATCAGAAACA+TGG | + | chr1.4:83737312-83737331 | None:intergenic | 50.0% | |
| ATTGGTTACCTTCGCCACCA+AGG | - | chr1.4:83736450-83736469 | MS.gene73434:intron | 50.0% | |
| CCATCGAGGCACACGATATT+GGG | + | chr1.4:83736093-83736112 | None:intergenic | 50.0% | |
| CTTCCATTTCAAGGCTGGCA+GGG | + | chr1.4:83737550-83737569 | None:intergenic | 50.0% | |
| GGCCCATAAGAAGACTGATG+AGG | - | chr1.4:83736484-83736503 | MS.gene73434:CDS | 50.0% | |
| GGTGGAAACAGTGAATGTGG+TGG | - | chr1.4:83736412-83736431 | MS.gene73434:intron | 50.0% | |
| TCTTCCATTTCAAGGCTGGC+AGG | + | chr1.4:83737551-83737570 | None:intergenic | 50.0% | |
| TGGACCTCGAGGATGATGAT+TGG | - | chr1.4:83736432-83736451 | MS.gene73434:intron | 50.0% | |
| TTCCATTTCAAGGCTGGCAG+GGG | + | chr1.4:83737549-83737568 | None:intergenic | 50.0% | |
| TTGGTTACCTTCGCCACCAA+GGG | - | chr1.4:83736451-83736470 | MS.gene73434:intron | 50.0% | |
| AACCCCTGCCAGCCTTGAAA+TGG | - | chr1.4:83737544-83737563 | MS.gene73434:CDS | 55.0% | |
| CCCAATATCGTGTGCCTCGA+TGG | - | chr1.4:83736090-83736109 | MS.gene73434:CDS | 55.0% | |
| TCACTTACGTCGTCCACGGA+AGG | + | chr1.4:83737090-83737109 | None:intergenic | 55.0% | |
| !! | AGTGAATGTGGTGGACCTCG+AGG | - | chr1.4:83736421-83736440 | MS.gene73434:intron | 55.0% |
| CCTTCGCCACCAAGGGTCAC+CGG | - | chr1.4:83736458-83736477 | MS.gene73434:CDS | 65.0% | |
| GCCACCAAGGGTCACCGGTA+AGG | - | chr1.4:83736463-83736482 | MS.gene73434:CDS | 65.0% | |
| GCCTTACCGGTGACCCTTGG+TGG | + | chr1.4:83736467-83736486 | None:intergenic | 65.0% | |
| TGGGCCTTACCGGTGACCCT+TGG | + | chr1.4:83736470-83736489 | None:intergenic | 65.0% | |
| !! | CCGGTGACCCTTGGTGGCGA+AGG | + | chr1.4:83736461-83736480 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 83736043 | 83737607 | 83736043 | ID=MS.gene73434 |
| chr1.4 | mRNA | 83736043 | 83737607 | 83736043 | ID=MS.gene73434.t1;Parent=MS.gene73434 |
| chr1.4 | exon | 83737539 | 83737607 | 83737539 | ID=MS.gene73434.t1.exon1;Parent=MS.gene73434.t1 |
| chr1.4 | CDS | 83737539 | 83737607 | 83737539 | ID=cds.MS.gene73434.t1;Parent=MS.gene73434.t1 |
| chr1.4 | exon | 83737119 | 83737261 | 83737119 | ID=MS.gene73434.t1.exon2;Parent=MS.gene73434.t1 |
| chr1.4 | CDS | 83737119 | 83737261 | 83737119 | ID=cds.MS.gene73434.t1;Parent=MS.gene73434.t1 |
| chr1.4 | exon | 83736456 | 83736663 | 83736456 | ID=MS.gene73434.t1.exon3;Parent=MS.gene73434.t1 |
| chr1.4 | CDS | 83736456 | 83736663 | 83736456 | ID=cds.MS.gene73434.t1;Parent=MS.gene73434.t1 |
| chr1.4 | exon | 83736043 | 83736210 | 83736043 | ID=MS.gene73434.t1.exon4;Parent=MS.gene73434.t1 |
| chr1.4 | CDS | 83736043 | 83736210 | 83736043 | ID=cds.MS.gene73434.t1;Parent=MS.gene73434.t1 |
| Gene Sequence |
| Protein sequence |