Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73520.t1 | XP_003624883.1 | 97.3 | 110 | 3 | 0 | 7 | 116 | 94 | 203 | 3.80E-48 | 200.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73520.t1 | Q9M612 | 79.1 | 110 | 22 | 1 | 7 | 115 | 95 | 204 | 5.2e-38 | 158.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73520.t1 | G7L4T7 | 97.3 | 110 | 3 | 0 | 7 | 116 | 94 | 203 | 2.7e-48 | 200.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049850 | MS.gene73520 | 0.805576 | 1.25E-49 | -1.69E-46 |
| MS.gene050807 | MS.gene73520 | 0.804899 | 1.73E-49 | -1.69E-46 |
| MS.gene05157 | MS.gene73520 | 0.802704 | 4.94E-49 | -1.69E-46 |
| MS.gene051710 | MS.gene73520 | 0.802791 | 4.74E-49 | -1.69E-46 |
| MS.gene051744 | MS.gene73520 | 0.805831 | 1.10E-49 | -1.69E-46 |
| MS.gene052611 | MS.gene73520 | 0.801914 | 7.19E-49 | -1.69E-46 |
| MS.gene052863 | MS.gene73520 | 0.819232 | 1.29E-52 | -1.69E-46 |
| MS.gene054651 | MS.gene73520 | 0.800736 | 1.25E-48 | -1.69E-46 |
| MS.gene054775 | MS.gene73520 | 0.809929 | 1.48E-50 | -1.69E-46 |
| MS.gene054801 | MS.gene73520 | 0.829347 | 5.42E-55 | -1.69E-46 |
| MS.gene055466 | MS.gene73520 | 0.814874 | 1.23E-51 | -1.69E-46 |
| MS.gene055818 | MS.gene73520 | 0.814505 | 1.49E-51 | -1.69E-46 |
| MS.gene056553 | MS.gene73520 | 0.808941 | 2.42E-50 | -1.69E-46 |
| MS.gene057915 | MS.gene73520 | 0.819962 | 8.82E-53 | -1.69E-46 |
| MS.gene058010 | MS.gene73520 | 0.810999 | 8.72E-51 | -1.69E-46 |
| MS.gene058625 | MS.gene73520 | 0.808022 | 3.80E-50 | -1.69E-46 |
| MS.gene059439 | MS.gene73520 | 0.805438 | 1.33E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73520.t1 | MTR_7g088680 | 97.273 | 110 | 3 | 0 | 7 | 116 | 94 | 203 | 2.14e-73 | 217 |
| MS.gene73520.t1 | MTR_3g092970 | 66.379 | 116 | 33 | 2 | 7 | 116 | 112 | 227 | 7.31e-43 | 140 |
| MS.gene73520.t1 | MTR_3g092980 | 55.285 | 123 | 34 | 2 | 7 | 116 | 104 | 218 | 3.65e-36 | 123 |
| MS.gene73520.t1 | MTR_3g092980 | 55.285 | 123 | 34 | 2 | 7 | 116 | 106 | 220 | 4.02e-36 | 122 |
| MS.gene73520.t1 | MTR_3g093750 | 54.464 | 112 | 40 | 3 | 7 | 116 | 425 | 527 | 1.04e-25 | 100 |
| MS.gene73520.t1 | MTR_3g093720 | 76.744 | 43 | 10 | 0 | 74 | 116 | 215 | 257 | 4.19e-15 | 69.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73520.t1 | AT3G12390 | 74.775 | 111 | 26 | 1 | 7 | 115 | 92 | 202 | 3.52e-43 | 140 |
| MS.gene73520.t1 | AT5G13850 | 70.690 | 116 | 27 | 1 | 7 | 115 | 88 | 203 | 2.21e-41 | 135 |
| MS.gene73520.t1 | AT3G49470 | 60.345 | 116 | 40 | 1 | 7 | 116 | 102 | 217 | 5.33e-39 | 130 |
| MS.gene73520.t1 | AT1G33040 | 59.130 | 115 | 41 | 2 | 7 | 115 | 94 | 208 | 7.62e-37 | 124 |
| MS.gene73520.t1 | AT4G10480 | 62.393 | 117 | 34 | 2 | 7 | 115 | 96 | 210 | 2.48e-36 | 123 |
| MS.gene73520.t1 | AT4G10480 | 62.393 | 117 | 34 | 2 | 7 | 115 | 97 | 211 | 2.87e-36 | 123 |
| MS.gene73520.t1 | AT3G49470 | 58.879 | 107 | 38 | 1 | 7 | 107 | 102 | 208 | 1.47e-33 | 116 |
Find 27 sgRNAs with CRISPR-Local
Find 30 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTGACACCTACATTATATT+TGG | 0.098409 | 4.4:+64690697 | MS.gene73520:CDS |
| GGAGCAATACCAGAGGATTC+TGG | 0.324111 | 4.4:-64690811 | None:intergenic |
| TCGTCTACATCCTCATCTTC+TGG | 0.324398 | 4.4:-64690832 | None:intergenic |
| CTGACACCTACATTATATTT+GGG | 0.330139 | 4.4:+64690698 | MS.gene73520:CDS |
| GCACCCGCATTGGTCAAATT+AGG | 0.353452 | 4.4:-64690784 | None:intergenic |
| GCTTTAACAGCTCTTGATCT+TGG | 0.356165 | 4.4:-64690907 | None:intergenic |
| AGACATTGTTGCTGCCATTA+TGG | 0.359211 | 4.4:+64690948 | MS.gene73520:CDS |
| GTTGGACTCTTGAAAACATC+TGG | 0.370830 | 4.4:-64690673 | None:intergenic |
| TTAATTTGTTAGCTCCATAA+TGG | 0.425574 | 4.4:-64690962 | None:intergenic |
| CACCACCAACTCAATATCCT+TGG | 0.437037 | 4.4:-64690869 | None:intergenic |
| TTCTGGCTTCGCACCCGCAT+TGG | 0.469966 | 4.4:-64690794 | None:intergenic |
| TCAGGCTGCAGAGCAGTTCA+AGG | 0.470685 | 4.4:+64690759 | MS.gene73520:CDS |
| AAAGCTCTCAAAGCTGTCAA+TGG | 0.508744 | 4.4:+64690925 | MS.gene73520:CDS |
| ATAATGTAGGTGTCAGAAGT+TGG | 0.511639 | 4.4:-64690691 | None:intergenic |
| TCCCAAGGATATTGAGTTGG+TGG | 0.515624 | 4.4:+64690867 | MS.gene73520:CDS |
| GCTCCTAATTTGACCAATGC+GGG | 0.519514 | 4.4:+64690781 | MS.gene73520:CDS |
| ACCACCAACTCAATATCCTT+GGG | 0.531886 | 4.4:-64690868 | None:intergenic |
| GGTGCGAAGCCAGAATCCTC+TGG | 0.535151 | 4.4:+64690802 | MS.gene73520:CDS |
| GATGAGGATGTAGACGAGAC+TGG | 0.538671 | 4.4:+64690838 | MS.gene73520:CDS |
| GAGTTGGTGGTGACTCAAGC+TGG | 0.556994 | 4.4:+64690880 | MS.gene73520:CDS |
| AGCTTTCCCAAATATAATGT+AGG | 0.558527 | 4.4:-64690704 | None:intergenic |
| GAGCTCACAGCTACAAACTC+AGG | 0.597975 | 4.4:+64690741 | MS.gene73520:CDS |
| CGAGACTGGTGTAGATCCCA+AGG | 0.607645 | 4.4:+64690852 | MS.gene73520:CDS |
| TGGTATTGCTCCAGAAGATG+AGG | 0.607813 | 4.4:+64690822 | MS.gene73520:CDS |
| AGATCCCAAGGATATTGAGT+TGG | 0.629225 | 4.4:+64690864 | MS.gene73520:CDS |
| GGCTCCTAATTTGACCAATG+CGG | 0.643666 | 4.4:+64690780 | MS.gene73520:CDS |
| ATCTTCTGGAGCAATACCAG+AGG | 0.670950 | 4.4:-64690818 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATATTTTTTTCTTCTTCTTT+TGG | + | chr4.4:64690587-64690606 | MS.gene73520:intron | 15.0% |
| !!! | TTAATTTGTTAGCTCCATAA+TGG | - | chr4.4:64690965-64690984 | None:intergenic | 25.0% |
| !!! | TTTATTTTTTGCATGAACTG+AGG | + | chr4.4:64690547-64690566 | MS.gene73520:intron | 25.0% |
| AGCTTTCCCAAATATAATGT+AGG | - | chr4.4:64690707-64690726 | None:intergenic | 30.0% | |
| CTGACACCTACATTATATTT+GGG | + | chr4.4:64690698-64690717 | MS.gene73520:CDS | 30.0% | |
| TCTGACACCTACATTATATT+TGG | + | chr4.4:64690697-64690716 | MS.gene73520:CDS | 30.0% | |
| ATAATGTAGGTGTCAGAAGT+TGG | - | chr4.4:64690694-64690713 | None:intergenic | 35.0% | |
| AAAGCTCTCAAAGCTGTCAA+TGG | + | chr4.4:64690925-64690944 | MS.gene73520:CDS | 40.0% | |
| ACCACCAACTCAATATCCTT+GGG | - | chr4.4:64690871-64690890 | None:intergenic | 40.0% | |
| AGACATTGTTGCTGCCATTA+TGG | + | chr4.4:64690948-64690967 | MS.gene73520:CDS | 40.0% | |
| AGATCCCAAGGATATTGAGT+TGG | + | chr4.4:64690864-64690883 | MS.gene73520:CDS | 40.0% | |
| ATGAACTGAGGCTACAATGA+TGG | + | chr4.4:64690559-64690578 | MS.gene73520:intron | 40.0% | |
| GCTTTAACAGCTCTTGATCT+TGG | - | chr4.4:64690910-64690929 | None:intergenic | 40.0% | |
| GTTGGACTCTTGAAAACATC+TGG | - | chr4.4:64690676-64690695 | None:intergenic | 40.0% | |
| CACCACCAACTCAATATCCT+TGG | - | chr4.4:64690872-64690891 | None:intergenic | 45.0% | |
| GCTCCTAATTTGACCAATGC+GGG | + | chr4.4:64690781-64690800 | MS.gene73520:CDS | 45.0% | |
| GGCTCCTAATTTGACCAATG+CGG | + | chr4.4:64690780-64690799 | MS.gene73520:CDS | 45.0% | |
| TCGTCTACATCCTCATCTTC+TGG | - | chr4.4:64690835-64690854 | None:intergenic | 45.0% | |
| TGGTATTGCTCCAGAAGATG+AGG | + | chr4.4:64690822-64690841 | MS.gene73520:CDS | 45.0% | |
| !! | ATCTTCTGGAGCAATACCAG+AGG | - | chr4.4:64690821-64690840 | None:intergenic | 45.0% |
| !! | TCCCAAGGATATTGAGTTGG+TGG | + | chr4.4:64690867-64690886 | MS.gene73520:CDS | 45.0% |
| GAGCTCACAGCTACAAACTC+AGG | + | chr4.4:64690741-64690760 | MS.gene73520:CDS | 50.0% | |
| GATGAGGATGTAGACGAGAC+TGG | + | chr4.4:64690838-64690857 | MS.gene73520:CDS | 50.0% | |
| GGAGCAATACCAGAGGATTC+TGG | - | chr4.4:64690814-64690833 | None:intergenic | 50.0% | |
| ! | GCACCCGCATTGGTCAAATT+AGG | - | chr4.4:64690787-64690806 | None:intergenic | 50.0% |
| CGAGACTGGTGTAGATCCCA+AGG | + | chr4.4:64690852-64690871 | MS.gene73520:CDS | 55.0% | |
| TCAGGCTGCAGAGCAGTTCA+AGG | + | chr4.4:64690759-64690778 | MS.gene73520:CDS | 55.0% | |
| !! | GAGTTGGTGGTGACTCAAGC+TGG | + | chr4.4:64690880-64690899 | MS.gene73520:CDS | 55.0% |
| GGTGCGAAGCCAGAATCCTC+TGG | + | chr4.4:64690802-64690821 | MS.gene73520:CDS | 60.0% | |
| !! | TTCTGGCTTCGCACCCGCAT+TGG | - | chr4.4:64690797-64690816 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 64690523 | 64690984 | 64690523 | ID=MS.gene73520 |
| chr4.4 | mRNA | 64690523 | 64690984 | 64690523 | ID=MS.gene73520.t1;Parent=MS.gene73520 |
| chr4.4 | exon | 64690523 | 64690540 | 64690523 | ID=MS.gene73520.t1.exon1;Parent=MS.gene73520.t1 |
| chr4.4 | CDS | 64690523 | 64690540 | 64690523 | ID=cds.MS.gene73520.t1;Parent=MS.gene73520.t1 |
| chr4.4 | exon | 64690652 | 64690984 | 64690652 | ID=MS.gene73520.t1.exon2;Parent=MS.gene73520.t1 |
| chr4.4 | CDS | 64690652 | 64690984 | 64690652 | ID=cds.MS.gene73520.t1;Parent=MS.gene73520.t1 |
| Gene Sequence |
| Protein sequence |
