Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74337.t1 | XP_013443645.1 | 88.9 | 315 | 34 | 1 | 1 | 315 | 1 | 314 | 2.50E-142 | 515 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74337.t1 | Q337C0 | 24.0 | 175 | 115 | 4 | 59 | 217 | 56 | 228 | 4.9e-06 | 53.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74337.t1 | A0A072TVX0 | 88.9 | 315 | 34 | 1 | 1 | 315 | 1 | 314 | 1.8e-142 | 515.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050379 | MS.gene74337 | 0.821521 | 3.86E-53 | -1.69E-46 |
| MS.gene051959 | MS.gene74337 | 0.826922 | 2.08E-54 | -1.69E-46 |
| MS.gene051960 | MS.gene74337 | 0.827533 | 1.48E-54 | -1.69E-46 |
| MS.gene052053 | MS.gene74337 | 0.839238 | 1.79E-57 | -1.69E-46 |
| MS.gene053932 | MS.gene74337 | 0.815953 | 7.10E-52 | -1.69E-46 |
| MS.gene055150 | MS.gene74337 | 0.813519 | 2.46E-51 | -1.69E-46 |
| MS.gene056530 | MS.gene74337 | 0.82546 | 4.63E-54 | -1.69E-46 |
| MS.gene057001 | MS.gene74337 | 0.812458 | 4.20E-51 | -1.69E-46 |
| MS.gene060237 | MS.gene74337 | 0.839983 | 1.14E-57 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74337.t1 | MTR_0003s0580 | 90.476 | 315 | 29 | 1 | 1 | 315 | 1 | 314 | 0.0 | 547 |
| MS.gene74337.t1 | MTR_3g028060 | 51.020 | 294 | 140 | 4 | 21 | 312 | 15 | 306 | 5.19e-80 | 246 |
| MS.gene74337.t1 | MTR_3g034370 | 49.780 | 227 | 112 | 2 | 86 | 312 | 48 | 272 | 1.99e-69 | 217 |
| MS.gene74337.t1 | MTR_3g034410 | 41.581 | 291 | 124 | 7 | 21 | 309 | 15 | 261 | 1.56e-53 | 176 |
| MS.gene74337.t1 | MTR_3g034540 | 44.628 | 121 | 66 | 1 | 192 | 312 | 1 | 120 | 6.10e-26 | 100 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 49 sgRNAs with CRISPR-Local
Find 59 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGGAAATCCTATCCTTATTT+TGG | 0.090774 | 4.3:+60101022 | MS.gene74337:CDS |
| TTCGGCTCGGAAAACCTTTA+AGG | 0.152443 | 4.3:-60100953 | None:intergenic |
| AGTTGTTTGAAAACAAAATT+TGG | 0.202695 | 4.3:+60101318 | MS.gene74337:CDS |
| TAGATTATCCAATGAGTAAA+TGG | 0.228369 | 4.3:+60101109 | MS.gene74337:CDS |
| AAATGAAGATCATTGGCTTC+TGG | 0.247620 | 4.3:+60101418 | MS.gene74337:CDS |
| TCCAGCTTGTTGTAGAGTTT+TGG | 0.249224 | 4.3:-60100874 | None:intergenic |
| TGAGTTATCAGCATCCTTAA+AGG | 0.251066 | 4.3:+60100939 | MS.gene74337:CDS |
| GATCAATCAACTCAGTTCTA+AGG | 0.257308 | 4.3:-60101752 | None:intergenic |
| GAGGTTAAGAAAATGGTAAA+TGG | 0.279558 | 4.3:+60101492 | MS.gene74337:CDS |
| CTGATGCTATTTCGTTAATC+AGG | 0.307616 | 4.3:-60100984 | None:intergenic |
| TTATCCATGGTAATACTCTT+AGG | 0.320589 | 4.3:+60100841 | None:intergenic |
| ATATATTGAGGTTAAGAAAA+TGG | 0.326568 | 4.3:+60101485 | MS.gene74337:CDS |
| AATGAAGATCATTGGCTTCT+GGG | 0.329863 | 4.3:+60101419 | MS.gene74337:CDS |
| AAATGATGCAGCAAAGGAAT+TGG | 0.358292 | 4.3:+60101650 | MS.gene74337:CDS |
| ACATTTATGAATCCAAAATA+AGG | 0.368400 | 4.3:-60101034 | None:intergenic |
| ATCATTGGCTTCTGGGTTTG+TGG | 0.371095 | 4.3:+60101426 | MS.gene74337:CDS |
| AAAATGGTAAATGGGTTTGA+TGG | 0.372096 | 4.3:+60101501 | MS.gene74337:CDS |
| AGCAAAGGAATTGGAGATAA+AGG | 0.392312 | 4.3:+60101659 | MS.gene74337:CDS |
| TCAACCTCCCATTTACTCAT+TGG | 0.398793 | 4.3:-60101117 | None:intergenic |
| TGTAAAAGCCTTGTTGATCA+AGG | 0.403671 | 4.3:-60101070 | None:intergenic |
| AGGTTAAGAAAATGGTAAAT+GGG | 0.412672 | 4.3:+60101493 | MS.gene74337:CDS |
| GTTAATCAGGTTCGAAACTT+CGG | 0.433919 | 4.3:-60100971 | None:intergenic |
| GTTTGTGGAATCTTATTGTC+TGG | 0.447627 | 4.3:+60101441 | MS.gene74337:CDS |
| CTGATAACTCATGAGATTCA+TGG | 0.455666 | 4.3:-60100927 | None:intergenic |
| GGTGATGATTGTTGTGGTGG+TGG | 0.459006 | 4.3:-60100906 | None:intergenic |
| CTGAGTTGATTGATCAACTA+AGG | 0.462243 | 4.3:+60101760 | MS.gene74337:CDS |
| AAGGATAGGATTTCCGATCC+TGG | 0.467137 | 4.3:-60101015 | None:intergenic |
| AAGTTTGTGAAGGTATTTGA+TGG | 0.483946 | 4.3:+60101687 | MS.gene74337:CDS |
| ACTGATCTTGAAGGAGGTAA+AGG | 0.496235 | 4.3:+60101578 | MS.gene74337:CDS |
| GTAGTTGAGATACTCTTCAA+TGG | 0.502635 | 4.3:-60101142 | None:intergenic |
| AGATTATCCAATGAGTAAAT+GGG | 0.511538 | 4.3:+60101110 | MS.gene74337:CDS |
| GTGAATGAAATGAAGATCAT+TGG | 0.513061 | 4.3:+60101411 | MS.gene74337:CDS |
| GTAAAAGCCTTGTTGATCAA+GGG | 0.513853 | 4.3:-60101069 | None:intergenic |
| TATGAATCCAAAATAAGGAT+AGG | 0.519500 | 4.3:-60101029 | None:intergenic |
| TGATGTTAAGCCATATATTG+AGG | 0.527762 | 4.3:+60101473 | MS.gene74337:CDS |
| CATGGTGATGATTGTTGTGG+TGG | 0.538232 | 4.3:-60100909 | None:intergenic |
| ATTGATTCTAAAATCAGTGA+AGG | 0.542847 | 4.3:+60101540 | MS.gene74337:CDS |
| TCCAAAACTCTACAACAAGC+TGG | 0.546418 | 4.3:+60100873 | MS.gene74337:CDS |
| ATCAATCAACTCAGTTCTAA+GGG | 0.562320 | 4.3:-60101751 | None:intergenic |
| TTGATTCTAAAATCAGTGAA+GGG | 0.585790 | 4.3:+60101541 | MS.gene74337:CDS |
| ATAGCATCAGATTCGAAACC+AGG | 0.598679 | 4.3:+60100997 | MS.gene74337:CDS |
| ATCAGATTCGAAACCAGGAT+CGG | 0.606501 | 4.3:+60101002 | MS.gene74337:CDS |
| AAAGGTGAACAAGTTTGTGA+AGG | 0.611343 | 4.3:+60101677 | MS.gene74337:CDS |
| ATTTGATGGTGTAAAAGCAG+AGG | 0.615999 | 4.3:+60101701 | MS.gene74337:CDS |
| ATTCATGGTGATGATTGTTG+TGG | 0.621498 | 4.3:-60100912 | None:intergenic |
| AATCTTACCCTTGATCAACA+AGG | 0.636503 | 4.3:+60101062 | MS.gene74337:CDS |
| TCAGGTTCGAAACTTCGGCT+CGG | 0.644764 | 4.3:-60100966 | None:intergenic |
| GTGTCCTAAGAGTATTACCA+TGG | 0.645337 | 4.3:-60100845 | None:intergenic |
| TTATCCAATGAGTAAATGGG+AGG | 0.684960 | 4.3:+60101113 | MS.gene74337:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | CATAAAATAAGGTTTTTAAA+AGG | + | chr4.3:60101363-60101382 | MS.gene74337:CDS | 15.0% |
| !! | ACATTTATGAATCCAAAATA+AGG | - | chr4.3:60101037-60101056 | None:intergenic | 20.0% |
| !! | AGTTGTTTGAAAACAAAATT+TGG | + | chr4.3:60101318-60101337 | MS.gene74337:CDS | 20.0% |
| !! | ATATATTGAGGTTAAGAAAA+TGG | + | chr4.3:60101485-60101504 | MS.gene74337:CDS | 20.0% |
| !! | GTGAAAAAAGTCATAAAATA+AGG | + | chr4.3:60101352-60101371 | MS.gene74337:CDS | 20.0% |
| !!! | AATAAGGTTTTTAAAAGGTA+AGG | + | chr4.3:60101368-60101387 | MS.gene74337:CDS | 20.0% |
| !!! | AATTTTGTTTTCAAACAACT+TGG | - | chr4.3:60101318-60101337 | None:intergenic | 20.0% |
| !!! | ATTTTCTTAACCTCAATATA+TGG | - | chr4.3:60101486-60101505 | None:intergenic | 20.0% |
| ! | AGATTATCCAATGAGTAAAT+GGG | + | chr4.3:60101110-60101129 | MS.gene74337:CDS | 25.0% |
| ! | AGGTTAAGAAAATGGTAAAT+GGG | + | chr4.3:60101493-60101512 | MS.gene74337:CDS | 25.0% |
| ! | TAGATTATCCAATGAGTAAA+TGG | + | chr4.3:60101109-60101128 | MS.gene74337:CDS | 25.0% |
| ! | TATGAATCCAAAATAAGGAT+AGG | - | chr4.3:60101032-60101051 | None:intergenic | 25.0% |
| !! | TTTTGGATTCATAAATGTCT+TGG | + | chr4.3:60101039-60101058 | MS.gene74337:CDS | 25.0% |
| !!! | ATTGATTCTAAAATCAGTGA+AGG | + | chr4.3:60101540-60101559 | MS.gene74337:CDS | 25.0% |
| !!! | TGATCTTTTTTCTAAGGTAA+TGG | + | chr4.3:60101728-60101747 | MS.gene74337:CDS | 25.0% |
| !!! | TTGATTCTAAAATCAGTGAA+GGG | + | chr4.3:60101541-60101560 | MS.gene74337:CDS | 25.0% |
| GAGGTTAAGAAAATGGTAAA+TGG | + | chr4.3:60101492-60101511 | MS.gene74337:CDS | 30.0% | |
| GTGAATGAAATGAAGATCAT+TGG | + | chr4.3:60101411-60101430 | MS.gene74337:CDS | 30.0% | |
| TGAGAAAAAACTGATCTTGA+AGG | + | chr4.3:60101569-60101588 | MS.gene74337:CDS | 30.0% | |
| TGATGTTAAGCCATATATTG+AGG | + | chr4.3:60101473-60101492 | MS.gene74337:CDS | 30.0% | |
| ! | AAAATGGTAAATGGGTTTGA+TGG | + | chr4.3:60101501-60101520 | MS.gene74337:CDS | 30.0% |
| ! | ATCAATCAACTCAGTTCTAA+GGG | - | chr4.3:60101754-60101773 | None:intergenic | 30.0% |
| ! | GGCTTTTACAAAGTTTACTT+TGG | + | chr4.3:60101083-60101102 | MS.gene74337:CDS | 30.0% |
| !! | AAGTTTGTGAAGGTATTTGA+TGG | + | chr4.3:60101687-60101706 | MS.gene74337:CDS | 30.0% |
| !!! | GGTAGATGATCTTTTTTCTA+AGG | + | chr4.3:60101722-60101741 | MS.gene74337:CDS | 30.0% |
| AAAGGTGAACAAGTTTGTGA+AGG | + | chr4.3:60101677-60101696 | MS.gene74337:CDS | 35.0% | |
| AAATGATGCAGCAAAGGAAT+TGG | + | chr4.3:60101650-60101669 | MS.gene74337:CDS | 35.0% | |
| AATCTTACCCTTGATCAACA+AGG | + | chr4.3:60101062-60101081 | MS.gene74337:CDS | 35.0% | |
| AGCAAAGGAATTGGAGATAA+AGG | + | chr4.3:60101659-60101678 | MS.gene74337:CDS | 35.0% | |
| AGTGAAAAATGATGCAGCAA+AGG | + | chr4.3:60101644-60101663 | MS.gene74337:CDS | 35.0% | |
| CTGATAACTCATGAGATTCA+TGG | - | chr4.3:60100930-60100949 | None:intergenic | 35.0% | |
| GAAAAAACTGATCTTGAAGG+AGG | + | chr4.3:60101572-60101591 | MS.gene74337:CDS | 35.0% | |
| GATCAATCAACTCAGTTCTA+AGG | - | chr4.3:60101755-60101774 | None:intergenic | 35.0% | |
| GTAGTTGAGATACTCTTCAA+TGG | - | chr4.3:60101145-60101164 | None:intergenic | 35.0% | |
| GTTAATCAGGTTCGAAACTT+CGG | - | chr4.3:60100974-60100993 | None:intergenic | 35.0% | |
| GTTTGTGGAATCTTATTGTC+TGG | + | chr4.3:60101441-60101460 | MS.gene74337:CDS | 35.0% | |
| TGAGTTATCAGCATCCTTAA+AGG | + | chr4.3:60100939-60100958 | MS.gene74337:CDS | 35.0% | |
| TTATCCAATGAGTAAATGGG+AGG | + | chr4.3:60101113-60101132 | MS.gene74337:CDS | 35.0% | |
| ! | ATTCATGGTGATGATTGTTG+TGG | - | chr4.3:60100915-60100934 | None:intergenic | 35.0% |
| !! | AAATGAAGATCATTGGCTTC+TGG | + | chr4.3:60101418-60101437 | MS.gene74337:CDS | 35.0% |
| !! | AATGAAGATCATTGGCTTCT+GGG | + | chr4.3:60101419-60101438 | MS.gene74337:CDS | 35.0% |
| !! | ATTTGATGGTGTAAAAGCAG+AGG | + | chr4.3:60101701-60101720 | MS.gene74337:CDS | 35.0% |
| !! | CGGAAATCCTATCCTTATTT+TGG | + | chr4.3:60101022-60101041 | MS.gene74337:CDS | 35.0% |
| !! | CTGAGTTGATTGATCAACTA+AGG | + | chr4.3:60101760-60101779 | MS.gene74337:CDS | 35.0% |
| !! | CTGATGCTATTTCGTTAATC+AGG | - | chr4.3:60100987-60101006 | None:intergenic | 35.0% |
| !! | GTAAAAGCCTTGTTGATCAA+GGG | - | chr4.3:60101072-60101091 | None:intergenic | 35.0% |
| !! | TGTAAAAGCCTTGTTGATCA+AGG | - | chr4.3:60101073-60101092 | None:intergenic | 35.0% |
| ACTGATCTTGAAGGAGGTAA+AGG | + | chr4.3:60101578-60101597 | MS.gene74337:CDS | 40.0% | |
| ATAGCATCAGATTCGAAACC+AGG | + | chr4.3:60100997-60101016 | MS.gene74337:CDS | 40.0% | |
| ATCAGATTCGAAACCAGGAT+CGG | + | chr4.3:60101002-60101021 | MS.gene74337:CDS | 40.0% | |
| TCAACCTCCCATTTACTCAT+TGG | - | chr4.3:60101120-60101139 | None:intergenic | 40.0% | |
| TCCAAAACTCTACAACAAGC+TGG | + | chr4.3:60100873-60100892 | MS.gene74337:CDS | 40.0% | |
| !!! | TCCAGCTTGTTGTAGAGTTT+TGG | - | chr4.3:60100877-60100896 | None:intergenic | 40.0% |
| AAGGATAGGATTTCCGATCC+TGG | - | chr4.3:60101018-60101037 | None:intergenic | 45.0% | |
| ! | CATGGTGATGATTGTTGTGG+TGG | - | chr4.3:60100912-60100931 | None:intergenic | 45.0% |
| !! | ATCATTGGCTTCTGGGTTTG+TGG | + | chr4.3:60101426-60101445 | MS.gene74337:CDS | 45.0% |
| !! | TTCGGCTCGGAAAACCTTTA+AGG | - | chr4.3:60100956-60100975 | None:intergenic | 45.0% |
| GGTGATGATTGTTGTGGTGG+TGG | - | chr4.3:60100909-60100928 | None:intergenic | 50.0% | |
| TCAGGTTCGAAACTTCGGCT+CGG | - | chr4.3:60100969-60100988 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 60100847 | 60101794 | 60100847 | ID=MS.gene74337 |
| chr4.3 | mRNA | 60100847 | 60101794 | 60100847 | ID=MS.gene74337.t1;Parent=MS.gene74337 |
| chr4.3 | exon | 60100847 | 60101794 | 60100847 | ID=MS.gene74337.t1.exon1;Parent=MS.gene74337.t1 |
| chr4.3 | CDS | 60100847 | 60101794 | 60100847 | ID=cds.MS.gene74337.t1;Parent=MS.gene74337.t1 |
| Gene Sequence |
| Protein sequence |
