AlfalfaGEDB | MS.gene74975

M. sativa cultivar XinJiangDaYe / MS.gene74975

KEGG: K02639
GO: GO:0003674 GO:0003824 GO:0005488 GO:0006091 GO:0008150 GO:0008152 GO:0009055 GO:0009987 GO:0016491 GO:0022900 GO:0044237 GO:0048037 GO:0051536 GO:0051537 GO:0051540 GO:0055114
PFAM: PF00111.30

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene74975.t1	XP_013470269.1	97.4	154	4	0	1	154	1	154	3.30E-76	294.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene74975.t1	P27788	61.7	154	55	2	1	153	1	151	4.6e-42	172.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene74975.t1	I3SU95	97.4	154	4	0	1	154	1	154	2.4e-76	294.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene051255	MS.gene74975	-0.828225	1.01E-54	-1.69E-46
MS.gene051525	MS.gene74975	-0.81161	6.42E-51	-1.69E-46
MS.gene053169	MS.gene74975	-0.832681	8.23E-56	-1.69E-46
MS.gene05335	MS.gene74975	-0.802047	6.75E-49	-1.69E-46
MS.gene054010	MS.gene74975	-0.817257	3.62E-52	-1.69E-46
MS.gene054012	MS.gene74975	-0.803243	3.83E-49	-1.69E-46
MS.gene054556	MS.gene74975	-0.846267	2.42E-59	-1.69E-46
MS.gene056346	MS.gene74975	-0.808742	2.67E-50	-1.69E-46
MS.gene056902	MS.gene74975	-0.805571	1.25E-49	-1.69E-46
MS.gene058700	MS.gene74975	-0.823555	1.30E-53	-1.69E-46
MS.gene058948	MS.gene74975	-0.82448	7.89E-54	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene74975.t1	MTR_1g112050	97.403	154	4	0	1	154	1	154	2.88e-110	310
MS.gene74975.t1	MTR_1g112030	92.157	153	12	0	1	153	7	159	1.55e-101	288
MS.gene74975.t1	MTR_1g112030	92.157	153	12	0	1	153	3	155	1.64e-101	288
MS.gene74975.t1	MTR_2g086510	72.519	131	35	1	24	153	24	154	1.14e-64	194
MS.gene74975.t1	MTR_4g053120	63.636	154	52	2	1	153	4	154	4.45e-63	191
MS.gene74975.t1	MTR_2g006290	56.164	146	58	3	13	153	4	148	8.00e-50	157
MS.gene74975.t1	MTR_8g088720	55.782	147	62	3	9	153	1	146	1.62e-49	156
MS.gene74975.t1	MTR_5g006320	35.200	125	79	2	29	153	21	143	1.71e-19	79.7
MS.gene74975.t1	MTR_4g098490	38.824	85	52	0	69	153	74	158	2.73e-17	75.1

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene74975.t1	AT2G27510	59.740	154	56	3	6	153	1	154	6.72e-59	180
MS.gene74975.t1	AT1G60950	61.417	127	43	3	32	153	22	147	1.56e-50	159
MS.gene74975.t1	AT1G10960	59.690	129	46	3	30	153	20	147	6.37e-48	152
MS.gene74975.t1	AT5G10000	54.701	117	50	2	40	153	31	147	7.22e-39	129
MS.gene74975.t1	AT4G14890	35.135	148	92	4	6	153	1	144	1.01e-19	80.5
MS.gene74975.t1	AT1G32550	40.000	85	51	0	69	153	69	153	3.41e-17	74.7
MS.gene74975.t1	AT1G32550	37.624	101	44	3	69	153	69	166	8.95e-12	60.5

Find 36 sgRNAs with CRISPR-Local

Find 37 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTGTGCCGGCCAAGTGGTTT+CGG	0.236759	1.3:-75981557	MS.gene74975:CDS
TAAATCACAGTATCTGATTT+CGG	0.282556	1.3:+75981454	None:intergenic
TTCACAGATCTCAAACTTGA+TGG	0.283462	1.3:+75981775	None:intergenic
AAATCACAGTATCTGATTTC+GGG	0.297921	1.3:+75981455	None:intergenic
TGTGCCGGCCAAGTGGTTTC+GGG	0.331646	1.3:-75981556	MS.gene74975:CDS
AAGAATGTGTCCAAAAGATT+TGG	0.383547	1.3:-75981754	MS.gene74975:CDS
CCCCGATGACTATTACATTC+TGG	0.413512	1.3:-75981641	MS.gene74975:CDS
TCCAGAATGTAATAGTCATC+GGG	0.445497	1.3:+75981640	None:intergenic
AATTCGTTCTCTGTACCATC+TGG	0.467589	1.3:+75981670	None:intergenic
CTACCTTACTCATGCAGAGC+TGG	0.470258	1.3:-75981592	MS.gene74975:CDS
TGCGCGGAACGGCTCCCTCT+AGG	0.480640	1.3:-75981822	MS.gene74975:CDS
CGAAACCACTTGGCCGGCAC+AGG	0.489725	1.3:+75981558	None:intergenic
CTGGATTCAGCTGAAGAAGC+TGG	0.504908	1.3:-75981622	MS.gene74975:CDS
GCACCAGCTCTGCATGAGTA+AGG	0.507611	1.3:+75981589	None:intergenic
AGAACCCGAAACCACTTGGC+CGG	0.523396	1.3:+75981552	None:intergenic
CGACGAGCAGCAAATTGAGA+AGG	0.535435	1.3:-75981503	MS.gene74975:CDS
CAACAGAACCCGAAACCACT+TGG	0.542417	1.3:+75981548	None:intergenic
GACAGCTTCTTTGCGCGGAA+CGG	0.550384	1.3:-75981833	MS.gene74975:CDS
ATCCAGAATGTAATAGTCAT+CGG	0.565321	1.3:+75981639	None:intergenic
CTCATTTAGAATCTCAGCAA+TGG	0.565752	1.3:-75981719	MS.gene74975:CDS
CATTGCTGAGATTCTAAATG+AGG	0.566187	1.3:+75981720	None:intergenic
TTCTACCTGTGCCGGCCAAG+TGG	0.569026	1.3:-75981563	MS.gene74975:CDS
GGTGCATGTTCTACCTGTGC+CGG	0.574784	1.3:-75981571	MS.gene74975:CDS
TTCAGCTGAAGAAGCTGGAG+TGG	0.581167	1.3:-75981617	MS.gene74975:CDS
CTTCCGACAGCTTCTTTGCG+CGG	0.592710	1.3:-75981838	MS.gene74975:CDS
AGAAGCTGTCGGAAGTCTCA+TGG	0.605132	1.3:+75981846	None:intergenic
AAGAGCACAAGATGTCCTAG+AGG	0.621822	1.3:+75981807	None:intergenic
GTGAAACTGATTCAACCAGA+TGG	0.628301	1.3:-75981685	MS.gene74975:CDS
GACGAGCAGCAAATTGAGAA+GGG	0.638971	1.3:-75981502	MS.gene74975:CDS
AGAGCACAAGATGTCCTAGA+GGG	0.645453	1.3:+75981808	None:intergenic
TACTGTGATTTACACACACA+AGG	0.647153	1.3:-75981443	MS.gene74975:CDS
GTTCCGCGCAAAGAAGCTGT+CGG	0.660034	1.3:+75981835	None:intergenic
CCAGAATGTAATAGTCATCG+GGG	0.687496	1.3:+75981641	None:intergenic
AGCTGTCGGAAGTCTCATGG+TGG	0.714654	1.3:+75981849	None:intergenic
CAGAATGTAATAGTCATCGG+GGG	0.715828	1.3:+75981642	None:intergenic
TGTGATTTACACACACAAGG+AGG	0.721823	1.3:-75981440	MS.gene74975:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	TAAATCACAGTATCTGATTT+CGG	+	chr1.3:75981843-75981862	None:intergenic	25.0%
!!!	AGATTTTAGTCCAAATCTTT+TGG	+	chr1.3:75981553-75981572	None:intergenic	25.0%
	AAATCACAGTATCTGATTTC+GGG	+	chr1.3:75981842-75981861	None:intergenic	30.0%
	AAGAATGTGTCCAAAAGATT+TGG	-	chr1.3:75981540-75981559	MS.gene74975:CDS	30.0%
	ATCCAGAATGTAATAGTCAT+CGG	+	chr1.3:75981658-75981677	None:intergenic	30.0%
	CATTGCTGAGATTCTAAATG+AGG	+	chr1.3:75981577-75981596	None:intergenic	35.0%
	TACTGTGATTTACACACACA+AGG	-	chr1.3:75981851-75981870	MS.gene74975:CDS	35.0%
	TCCAGAATGTAATAGTCATC+GGG	+	chr1.3:75981657-75981676	None:intergenic	35.0%
	TTCACAGATCTCAAACTTGA+TGG	+	chr1.3:75981522-75981541	None:intergenic	35.0%
!	CTCATTTAGAATCTCAGCAA+TGG	-	chr1.3:75981575-75981594	MS.gene74975:CDS	35.0%
	AATTCGTTCTCTGTACCATC+TGG	+	chr1.3:75981627-75981646	None:intergenic	40.0%
	CAGAATGTAATAGTCATCGG+GGG	+	chr1.3:75981655-75981674	None:intergenic	40.0%
	CCAGAATGTAATAGTCATCG+GGG	+	chr1.3:75981656-75981675	None:intergenic	40.0%
	TGTGATTTACACACACAAGG+AGG	-	chr1.3:75981854-75981873	MS.gene74975:CDS	40.0%
!	GTGAAACTGATTCAACCAGA+TGG	-	chr1.3:75981609-75981628	MS.gene74975:CDS	40.0%
	AGAGCACAAGATGTCCTAGA+GGG	+	chr1.3:75981489-75981508	None:intergenic	45.0%
	CCCCGATGACTATTACATTC+TGG	-	chr1.3:75981653-75981672	MS.gene74975:CDS	45.0%
	GACGAGCAGCAAATTGAGAA+GGG	-	chr1.3:75981792-75981811	MS.gene74975:CDS	45.0%
!	AAGAGCACAAGATGTCCTAG+AGG	+	chr1.3:75981490-75981509	None:intergenic	45.0%
	AGAAGCTGTCGGAAGTCTCA+TGG	+	chr1.3:75981451-75981470	None:intergenic	50.0%
	CAACAGAACCCGAAACCACT+TGG	+	chr1.3:75981749-75981768	None:intergenic	50.0%
	CGACGAGCAGCAAATTGAGA+AGG	-	chr1.3:75981791-75981810	MS.gene74975:CDS	50.0%
	CTACCTTACTCATGCAGAGC+TGG	-	chr1.3:75981702-75981721	MS.gene74975:CDS	50.0%
	CTGGATTCAGCTGAAGAAGC+TGG	-	chr1.3:75981672-75981691	MS.gene74975:CDS	50.0%
	TTCAGCTGAAGAAGCTGGAG+TGG	-	chr1.3:75981677-75981696	MS.gene74975:CDS	50.0%
	AGAACCCGAAACCACTTGGC+CGG	+	chr1.3:75981745-75981764	None:intergenic	55.0%
	AGCTGTCGGAAGTCTCATGG+TGG	+	chr1.3:75981448-75981467	None:intergenic	55.0%
	CTTCCGACAGCTTCTTTGCG+CGG	-	chr1.3:75981456-75981475	MS.gene74975:CDS	55.0%
	GACAGCTTCTTTGCGCGGAA+CGG	-	chr1.3:75981461-75981480	MS.gene74975:CDS	55.0%
	GCACCAGCTCTGCATGAGTA+AGG	+	chr1.3:75981708-75981727	None:intergenic	55.0%
	GGTGCATGTTCTACCTGTGC+CGG	-	chr1.3:75981723-75981742	MS.gene74975:CDS	55.0%
	GTTCCGCGCAAAGAAGCTGT+CGG	+	chr1.3:75981462-75981481	None:intergenic	55.0%
!	CTGTGCCGGCCAAGTGGTTT+CGG	-	chr1.3:75981737-75981756	MS.gene74975:CDS	60.0%
!	TGTGCCGGCCAAGTGGTTTC+GGG	-	chr1.3:75981738-75981757	MS.gene74975:CDS	60.0%
!	TTCTACCTGTGCCGGCCAAG+TGG	-	chr1.3:75981731-75981750	MS.gene74975:CDS	60.0%
	CGAAACCACTTGGCCGGCAC+AGG	+	chr1.3:75981739-75981758	None:intergenic	65.0%
	TGCGCGGAACGGCTCCCTCT+AGG	-	chr1.3:75981472-75981491	MS.gene74975:CDS	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.3	gene	75981426	75981890	75981426	ID=MS.gene74975
chr1.3	mRNA	75981426	75981890	75981426	ID=MS.gene74975.t1;Parent=MS.gene74975
chr1.3	exon	75981426	75981890	75981426	ID=MS.gene74975.t1.exon1;Parent=MS.gene74975.t1
chr1.3	CDS	75981426	75981890	75981426	ID=cds.MS.gene74975.t1;Parent=MS.gene74975.t1

Gene Sequence

>MS.gene74975

ATGTCAGCTGTGAATATGTCCACCATGAGACTTCCGACAGCTTCTTTGCGCGGAACGGCTCCCTCTAGGACATCTTGTGCTCTTCTGAAGAATCCATCAAGTTTGAGATCTGTGAAGAATGTGTCCAAAAGATTTGGACTAAAATCTTCCTCATTTAGAATCTCAGCAATGGCAGTATACAAAGTGAAACTGATTCAACCAGATGGTACAGAGAACGAATTTGATGCCCCCGATGACTATTACATTCTGGATTCAGCTGAAGAAGCTGGAGTGGAGCTACCTTACTCATGCAGAGCTGGTGCATGTTCTACCTGTGCCGGCCAAGTGGTTTCGGGTTCTGTTGATCAGTCTGATCAATCTTTTCTCGACGAGCAGCAAATTGAGAAGGGTTATCTTCTCACATGTGTCTCATACCCGAAATCAGATACTGTGATTTACACACACAAGGAGGAAGAACTCTGCTAA

Protein sequence

>MS.gene74975.t1

MSAVNMSTMRLPTASLRGTAPSRTSCALLKNPSSLRSVKNVSKRFGLKSSSFRISAMAVYKVKLIQPDGTENEFDAPDDYYILDSAEEAGVELPYSCRAGACSTCAGQVVSGSVDQSDQSFLDEQQIEKGYLLTCVSYPKSDTVIYTHKEEELC