AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene75277

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene75277.t1 MTR_7g096350 97.674 172 4 0 29 200 332 503 7.35e-120 349
MS.gene75277.t1 MTR_7g117670 53.804 184 66 3 32 199 382 562 3.18e-52 176
MS.gene75277.t1 MTR_8g027495 52.273 176 61 3 42 199 448 618 1.21e-49 170
MS.gene75277.t1 MTR_5g030430 41.791 201 78 2 33 198 474 670 1.20e-40 146
MS.gene75277.t1 MTR_8g067280 42.553 188 71 4 25 177 437 622 2.54e-38 139
MS.gene75277.t1 MTR_8g024790 38.953 172 80 3 32 178 271 442 8.47e-32 120
MS.gene75277.t1 MTR_8g024770 40.491 163 69 2 32 166 259 421 4.32e-31 117
MS.gene75277.t1 MTR_4g092700 54.688 64 29 0 50 113 156 219 4.56e-17 78.2
MS.gene75277.t1 MTR_5g014600 56.452 62 26 1 52 113 149 209 2.02e-16 76.3
MS.gene75277.t1 MTR_1g072320 50.769 65 32 0 52 116 470 534 1.77e-14 71.6
MS.gene75277.t1 MTR_4g097940 43.478 92 42 3 27 114 130 215 6.55e-14 69.3
MS.gene75277.t1 MTR_2g038040 48.101 79 30 3 53 120 53 131 1.15e-13 67.8
MS.gene75277.t1 MTR_4g097950 51.852 54 26 0 52 105 70 123 1.82e-13 67.4
MS.gene75277.t1 MTR_1g112680 46.053 76 36 1 32 107 302 372 1.82e-13 68.6
MS.gene75277.t1 MTR_2g010450 50.877 57 28 0 52 108 177 233 2.07e-13 68.2
MS.gene75277.t1 MTR_5g014520 42.466 73 40 1 35 107 142 212 1.35e-12 65.5
MS.gene75277.t1 MTR_3g498695 51.724 58 28 0 50 107 296 353 1.57e-12 65.9
MS.gene75277.t1 MTR_3g498695 51.724 58 28 0 50 107 296 353 1.57e-12 65.9
MS.gene75277.t1 MTR_7g083900 50.000 58 29 0 50 107 284 341 2.26e-12 65.5
MS.gene75277.t1 MTR_7g083900 50.000 58 29 0 50 107 284 341 2.30e-12 65.1
MS.gene75277.t1 MTR_4g097920 36.207 116 61 4 27 138 130 236 2.99e-12 64.7
MS.gene75277.t1 MTR_4g097920 36.207 116 61 4 27 138 115 221 3.00e-12 64.3
MS.gene75277.t1 MTR_8g098275 49.180 61 31 0 48 108 397 457 3.46e-12 64.7
MS.gene75277.t1 MTR_8g098275 49.180 61 31 0 48 108 240 300 3.53e-12 64.7
MS.gene75277.t1 MTR_8g098275 49.180 61 31 0 48 108 304 364 3.55e-12 64.7
MS.gene75277.t1 MTR_0246s0020 44.118 68 32 1 38 105 190 251 3.61e-12 64.3
MS.gene75277.t1 MTR_4g066380 45.205 73 36 1 33 105 138 206 3.62e-12 64.3
MS.gene75277.t1 MTR_4g067010 45.588 68 32 2 39 105 137 200 3.64e-12 64.3
MS.gene75277.t1 MTR_0246s0020 44.118 68 32 1 38 105 190 251 3.72e-12 64.3
MS.gene75277.t1 MTR_5g014640 31.405 121 58 2 39 138 145 261 3.84e-12 64.3
MS.gene75277.t1 MTR_2g104550 42.105 76 42 2 40 115 127 200 5.65e-12 63.5
MS.gene75277.t1 MTR_4g066460 35.714 112 66 2 33 142 140 247 1.03e-11 63.2
MS.gene75277.t1 MTR_2g084280 26.000 150 89 3 56 183 137 286 1.64e-11 62.4
MS.gene75277.t1 MTR_6g047550 50.000 54 27 0 52 105 173 226 1.67e-11 62.0
MS.gene75277.t1 MTR_0011s0260 50.000 54 27 0 52 105 183 236 2.60e-11 62.0
MS.gene75277.t1 MTR_2g104490 36.264 91 55 2 40 130 126 213 2.61e-11 61.6
MS.gene75277.t1 MTR_0011s0210 50.000 54 27 0 52 105 183 236 2.84e-11 62.0
MS.gene75277.t1 MTR_2g104490 36.264 91 55 2 40 130 126 213 4.16e-11 61.2
MS.gene75277.t1 MTR_4g057270 46.154 52 28 0 56 107 153 204 4.65e-11 61.2
MS.gene75277.t1 MTR_3g030700 41.429 70 37 1 38 107 186 251 7.46e-11 60.8
MS.gene75277.t1 MTR_0250s0040 50.000 54 27 0 52 105 88 141 7.86e-11 60.1
MS.gene75277.t1 MTR_5g014560 48.077 52 27 0 54 105 133 184 8.68e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene75277.t1 AT4G16430 63.218 174 62 2 27 199 295 467 1.97e-73 229
MS.gene75277.t1 AT2G46510 53.073 179 72 4 27 197 370 544 4.82e-56 186
MS.gene75277.t1 AT1G01260 51.497 167 73 3 35 199 416 576 9.29e-51 172
MS.gene75277.t1 AT1G01260 51.497 167 73 3 35 199 416 576 9.29e-51 172
MS.gene75277.t1 AT1G01260 51.497 167 73 3 35 199 416 576 9.29e-51 172
MS.gene75277.t1 AT4G17880 44.706 170 70 2 33 178 397 566 5.73e-39 140
MS.gene75277.t1 AT5G46760 44.000 175 68 3 33 178 396 569 3.37e-38 139
MS.gene75277.t1 AT1G32640 41.579 190 82 2 33 197 433 618 4.58e-38 139
MS.gene75277.t1 AT5G46830 39.241 158 74 4 43 178 334 491 3.31e-26 105
MS.gene75277.t1 AT4G00870 37.059 170 73 5 30 165 220 389 1.98e-21 91.3
MS.gene75277.t1 AT4G29930 26.816 179 107 3 31 190 38 211 9.06e-16 73.2
MS.gene75277.t1 AT4G29930 26.136 176 109 4 31 190 38 208 3.79e-15 71.6
MS.gene75277.t1 AT1G63650 46.269 67 36 0 48 114 401 467 7.81e-15 72.8
MS.gene75277.t1 AT1G63650 46.269 67 36 0 48 114 401 467 7.81e-15 72.8
MS.gene75277.t1 AT1G63650 46.269 67 36 0 48 114 401 467 7.81e-15 72.8
MS.gene75277.t1 AT4G37850 48.000 75 39 0 34 108 134 208 8.80e-15 71.6
MS.gene75277.t1 AT4G37850 48.000 75 39 0 34 108 134 208 8.80e-15 71.6
MS.gene75277.t1 AT5G57150 43.373 83 46 1 26 107 28 110 1.41e-14 69.7
MS.gene75277.t1 AT5G57150 46.053 76 35 1 32 107 40 109 1.49e-14 69.7
MS.gene75277.t1 AT5G57150 43.373 83 46 1 26 107 28 110 1.58e-14 69.7
MS.gene75277.t1 AT5G57150 46.053 76 35 1 32 107 40 109 2.27e-14 69.7
MS.gene75277.t1 AT5G57150 46.053 76 35 1 32 107 41 110 2.97e-14 69.7
MS.gene75277.t1 AT4G29930 38.542 96 52 2 31 124 38 128 6.12e-14 68.9
MS.gene75277.t1 AT4G29930 38.542 96 52 2 31 124 38 128 6.12e-14 68.9
MS.gene75277.t1 AT4G29930 38.542 96 52 2 31 124 38 128 7.50e-14 68.6
MS.gene75277.t1 AT4G29930 38.542 96 52 2 31 124 38 128 9.51e-14 67.0
MS.gene75277.t1 AT4G29930 42.857 77 39 1 31 107 38 109 1.55e-13 67.4
MS.gene75277.t1 AT2G22750 50.000 64 32 0 38 101 140 203 1.72e-13 68.2
MS.gene75277.t1 AT2G22750 50.000 64 32 0 38 101 140 203 1.77e-13 68.2
MS.gene75277.t1 AT2G22750 50.000 64 32 0 38 101 112 175 1.90e-13 67.8
MS.gene75277.t1 AT2G22750 50.000 64 32 0 38 101 112 175 1.97e-13 67.8
MS.gene75277.t1 AT2G22750 50.000 64 32 0 38 101 136 199 2.00e-13 67.8
MS.gene75277.t1 AT1G12860 53.448 58 27 0 50 107 265 322 2.39e-13 68.2
MS.gene75277.t1 AT1G12860 53.448 58 27 0 50 107 265 322 2.74e-13 68.2
MS.gene75277.t1 AT4G09820 44.595 74 30 1 32 105 354 416 2.79e-13 68.2
MS.gene75277.t1 AT3G26744 51.724 58 28 0 50 107 305 362 1.03e-12 66.2
MS.gene75277.t1 AT3G26744 51.724 58 28 0 50 107 305 362 1.04e-12 66.2
MS.gene75277.t1 AT3G26744 51.724 58 28 0 50 107 305 362 1.04e-12 66.2
MS.gene75277.t1 AT3G26744 51.724 58 28 0 50 107 305 362 1.04e-12 66.2
MS.gene75277.t1 AT2G16910 48.485 66 32 1 56 119 318 383 1.91e-12 65.5
MS.gene75277.t1 AT2G22770 43.590 78 41 1 46 120 123 200 2.46e-12 64.7
MS.gene75277.t1 AT5G41315 37.681 69 43 0 46 114 435 503 7.41e-12 63.9
MS.gene75277.t1 AT5G41315 37.681 69 43 0 46 114 443 511 8.02e-12 63.9
MS.gene75277.t1 AT5G41315 37.681 69 43 0 46 114 440 508 8.17e-12 63.9
MS.gene75277.t1 AT2G22760 36.782 87 50 2 23 108 93 175 6.84e-11 60.5
MS.gene75277.t1 AT2G22760 36.782 87 50 2 23 108 93 175 8.02e-11 60.5

Find 44 sgRNAs with CRISPR-Local

Find 110 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTAGATATCCACCCGGTTTC+TGG 0.115234 2.1:-44393363 MS.gene75277:CDS
ATGGACAAGGCTTCTCTTCT+TGG 0.203486 2.1:-44393540 MS.gene75277:CDS
TGTTCCTAACATTTCTAAAA+TGG 0.208602 2.1:-44393559 MS.gene75277:CDS
CCATAAGCTTGATCTTCTTC+TGG 0.258919 2.1:+44393490 None:intergenic
TCTTCTGGAGATCAGTGATA+TGG 0.305181 2.1:+44393505 None:intergenic
CCAGAAGAAGATCAAGCTTA+TGG 0.312383 2.1:-44393490 MS.gene75277:CDS
AGAAACCGGGTGGATATCTA+AGG 0.336740 2.1:+44393365 None:intergenic
TTAACTCGTGTCTTGCCTCT+TGG 0.395730 2.1:-44393711 MS.gene75277:CDS
ATGTATGAATTACTTTATCC+TGG 0.397717 2.1:+44393280 None:intergenic
GTTAGGAACAACAGCTCTTA+AGG 0.401789 2.1:+44393572 None:intergenic
ACTTTAACAATTCCAGAAAC+CGG 0.412032 2.1:+44393351 None:intergenic
GGTTCCTCTCGTCCATTGGC+AGG 0.413139 2.1:+44393654 None:intergenic
GCTGAAGATGGCGGTGGAAA+GGG 0.428855 2.1:-44394698 MS.gene75277:CDS
CTCTTAAGGCGTAAAATCTC+TGG 0.435497 2.1:+44393586 None:intergenic
CGCTGAAGATGGCGGTGGAA+AGG 0.437244 2.1:-44394699 MS.gene75277:CDS
GTCTGCAGCTTCGCTGAAGA+TGG 0.448216 2.1:-44394710 MS.gene75277:CDS
CAGCGGTTCCTCTCGTCCAT+TGG 0.461859 2.1:+44393650 None:intergenic
TTCCCCTCCTTGAGTTCTGA+TGG 0.471316 2.1:+44393254 None:intergenic
GGCCAATGTTTCAACTTCCC+AGG 0.496533 2.1:-44393298 MS.gene75277:CDS
CTTCTGGAGATCAGTGATAT+GGG 0.500322 2.1:+44393506 None:intergenic
ATCCTGGGAAGTTGAAACAT+TGG 0.527219 2.1:+44393296 None:intergenic
TGTATGAATTACTTTATCCT+GGG 0.549481 2.1:+44393281 None:intergenic
GCATCAAATTGTAGCACAAG+AGG 0.564604 2.1:-44393319 MS.gene75277:CDS
AGAGGGAGAAAGCCTGCCAA+TGG 0.566417 2.1:-44393666 MS.gene75277:CDS
ACATTCTCCATCAGAACTCA+AGG 0.569500 2.1:-44393261 MS.gene75277:CDS
CTTTAACAATTCCAGAAACC+GGG 0.569555 2.1:+44393352 None:intergenic
CTGAAGATGGCGGTGGAAAG+GGG 0.578881 2.1:-44394697 MS.gene75277:intron
TCCGCTTCAACATGATTCAG+CGG 0.585645 2.1:+44393633 None:intergenic
AGCTTCGCTGAAGATGGCGG+TGG 0.593052 2.1:-44394704 MS.gene75277:CDS
CTCCATCAGAACTCAAGGAG+GGG 0.593410 2.1:-44393256 MS.gene75277:CDS
TAACATTTCTAAAATGGACA+AGG 0.599447 2.1:-44393553 MS.gene75277:CDS
ACCGCTGAATCATGTTGAAG+CGG 0.600251 2.1:-44393634 MS.gene75277:CDS
GAAACCGGGTGGATATCTAA+GGG 0.600502 2.1:+44393366 None:intergenic
CTGCCCCTTAGATATCCACC+CGG 0.603470 2.1:-44393370 MS.gene75277:CDS
TGCAGCTTCGCTGAAGATGG+CGG 0.603673 2.1:-44394707 MS.gene75277:CDS
TCTCCATCAGAACTCAAGGA+GGG 0.623115 2.1:-44393257 MS.gene75277:CDS
TGAAAATCAATATCTTGCAA+CGG 0.623594 2.1:+44393426 None:intergenic
TCTTGCCTCTTGGAGACGAG+CGG 0.640402 2.1:-44393701 MS.gene75277:CDS
AAAGCCTGCCAATGGACGAG+AGG 0.650271 2.1:-44393658 MS.gene75277:CDS
TTCTCCATCAGAACTCAAGG+AGG 0.654759 2.1:-44393258 MS.gene75277:CDS
TGAATCATGTTGAAGCGGAG+AGG 0.659736 2.1:-44393629 MS.gene75277:CDS
GGCTTCCGCTCGTCTCCAAG+AGG 0.677239 2.1:+44393696 None:intergenic
TAACAATTCCAGAAACCGGG+TGG 0.692180 2.1:+44393355 None:intergenic
AAACCGGGTGGATATCTAAG+GGG 0.697036 2.1:+44393367 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATATAATAAAAATGTGTG+TGG - chr2.1:44393845-44393864 MS.gene75277:intron 15.0%
!! TTATCTATATTTATTAATGC+CGG - chr2.1:44393489-44393508 MS.gene75277:CDS 15.0%
!!! AAAAATCAATCTCTAAATTT+TGG - chr2.1:44394161-44394180 MS.gene75277:intron 15.0%
!! AACACAATAAAAATGATAAC+TGG - chr2.1:44393701-44393720 MS.gene75277:CDS 20.0%
!! ACTTGTAGTAATATTATTGA+GGG + chr2.1:44394053-44394072 None:intergenic 20.0%
!!! TGCATTTTTGTTTTTTGATT+TGG + chr2.1:44394094-44394113 None:intergenic 20.0%
! AAATTGAATTGTGATGACAA+TGG - chr2.1:44393541-44393560 MS.gene75277:CDS 25.0%
! ATGACATATTAATGCTAACA+AGG + chr2.1:44393461-44393480 None:intergenic 25.0%
! ATGTATGAATTACTTTATCC+TGG + chr2.1:44394645-44394664 None:intergenic 25.0%
! ATTTATTAATGCCGGTAAAA+TGG - chr2.1:44393497-44393516 MS.gene75277:CDS 25.0%
! CACTTGTAGTAATATTATTG+AGG + chr2.1:44394054-44394073 None:intergenic 25.0%
! CTCAATAATATTACTACAAG+TGG - chr2.1:44394052-44394071 MS.gene75277:intron 25.0%
! TAATATTATTGAGGGTTTGT+CGG + chr2.1:44394045-44394064 None:intergenic 25.0%
! TAGCAATGAGATATTATTGT+AGG + chr2.1:44393879-44393898 None:intergenic 25.0%
! TATGGAAACTGAAAAAAACA+TGG - chr2.1:44394450-44394469 MS.gene75277:intron 25.0%
! TCATATCATATGAGTAAGAA+AGG - chr2.1:44393595-44393614 MS.gene75277:CDS 25.0%
! TGAAAATCAATATCTTGCAA+CGG + chr2.1:44394499-44394518 None:intergenic 25.0%
! TGTATGAATTACTTTATCCT+GGG + chr2.1:44394644-44394663 None:intergenic 25.0%
! TTCTTAATTCTCGTATTTGA+AGG - chr2.1:44393675-44393694 MS.gene75277:CDS 25.0%
!! TAGAGATCACATCTTTTAAT+TGG - chr2.1:44393626-44393645 MS.gene75277:CDS 25.0%
!! TGTTCCTAACATTTCTAAAA+TGG - chr2.1:44394363-44394382 MS.gene75277:intron 25.0%
!!! TAACATTTCTAAAATGGACA+AGG - chr2.1:44394369-44394388 MS.gene75277:intron 25.0%
!!! TTGTCCATTTTAGAAATGTT+AGG + chr2.1:44394370-44394389 None:intergenic 25.0%
AAAAAACATGGCTGACAATA+AGG - chr2.1:44394462-44394481 MS.gene75277:intron 30.0%
AAAAACATGGCTGACAATAA+GGG - chr2.1:44394463-44394482 MS.gene75277:intron 30.0%
AAAAATGCACCAAAGATAGT+TGG - chr2.1:44394105-44394124 MS.gene75277:intron 30.0%
AAAATGCACCAAAGATAGTT+GGG - chr2.1:44394106-44394125 MS.gene75277:intron 30.0%
AACAAGGATAAAATGTCATG+AGG + chr2.1:44393445-44393464 None:intergenic 30.0%
AATATTATTGAGGGTTTGTC+GGG + chr2.1:44394044-44394063 None:intergenic 30.0%
ACAACATTACCATATAATGG+AGG + chr2.1:44393382-44393401 None:intergenic 30.0%
ACTTTAACAATTCCAGAAAC+CGG + chr2.1:44394574-44394593 None:intergenic 30.0%
CAGACAACATTACCATATAA+TGG + chr2.1:44393385-44393404 None:intergenic 30.0%
GACAAGATAAACAAAGCAAA+GGG - chr2.1:44393766-44393785 MS.gene75277:intron 30.0%
GCAAGGAGTAATATTATTGA+AGG + chr2.1:44394022-44394041 None:intergenic 30.0%
TGACAAGATAAACAAAGCAA+AGG - chr2.1:44393765-44393784 MS.gene75277:intron 30.0%
! ATATGAGTAAGAAAGGCATA+AGG - chr2.1:44393602-44393621 MS.gene75277:CDS 30.0%
!! TGAAGAGTACAACTATTTTC+TGG + chr2.1:44393334-44393353 None:intergenic 30.0%
!!! CAATCTCTAAATTTTGGCTA+AGG - chr2.1:44394167-44394186 MS.gene75277:intron 30.0%
!!! GTTGCAAGATATTGATTTTC+AGG - chr2.1:44394498-44394517 MS.gene75277:intron 30.0%
!!! GTTTTTTGATTTGGTTTCCA+TGG + chr2.1:44394085-44394104 None:intergenic 30.0%
AAGCAACTATCCGATACTAA+CGG - chr2.1:44393907-44393926 MS.gene75277:intron 35.0%
ACACTAGAGACCCAATAAAA+AGG - chr2.1:44393731-44393750 MS.gene75277:CDS 35.0%
CACTAGAGACCCAATAAAAA+GGG - chr2.1:44393732-44393751 MS.gene75277:CDS 35.0%
CTTTAACAATTCCAGAAACC+GGG + chr2.1:44394573-44394592 None:intergenic 35.0%
TCTAGTGTTCAGACTAGATA+AGG - chr2.1:44393255-44393274 MS.gene75277:CDS 35.0%
TGGTCCTTGAAAATTAAGTC+CGG - chr2.1:44393561-44393580 MS.gene75277:CDS 35.0%
TTGAAAATTAAGTCCGGAGA+AGG - chr2.1:44393567-44393586 MS.gene75277:CDS 35.0%
TTGAGATAAGTATCCACACT+TGG - chr2.1:44393985-44394004 MS.gene75277:intron 35.0%
TTTAGCTAGACCGTTAGTAT+CGG + chr2.1:44393920-44393939 None:intergenic 35.0%
! ATGTTCTGCATGTTTCTGTT+AGG - chr2.1:44393353-44393372 MS.gene75277:CDS 35.0%
! TCCATGTGTCCCTTTTTATT+GGG + chr2.1:44393744-44393763 None:intergenic 35.0%
! TGTTCTGCATGTTTCTGTTA+GGG - chr2.1:44393354-44393373 MS.gene75277:CDS 35.0%
! TTCTCCGGACTTAATTTTCA+AGG + chr2.1:44393568-44393587 None:intergenic 35.0%
!! CTTCATTGTAGCCATTTTAC+CGG + chr2.1:44393511-44393530 None:intergenic 35.0%
!!! ATCGGATAGTTGCTTTTAAG+TGG + chr2.1:44393902-44393921 None:intergenic 35.0%
ACATTCTCCATCAGAACTCA+AGG - chr2.1:44394661-44394680 MS.gene75277:intron 40.0%
ACCCAATAAAAAGGGACACA+TGG - chr2.1:44393740-44393759 MS.gene75277:intron 40.0%
ATCCTGGGAAGTTGAAACAT+TGG + chr2.1:44394629-44394648 None:intergenic 40.0%
CCAGAAGAAGATCAAGCTTA+TGG - chr2.1:44394432-44394451 MS.gene75277:intron 40.0%
CCATAAGCTTGATCTTCTTC+TGG + chr2.1:44394435-44394454 None:intergenic 40.0%
CTCTTAAGGCGTAAAATCTC+TGG + chr2.1:44394339-44394358 None:intergenic 40.0%
GCATCAAATTGTAGCACAAG+AGG - chr2.1:44394603-44394622 MS.gene75277:intron 40.0%
GTTAGGAACAACAGCTCTTA+AGG + chr2.1:44394353-44394372 None:intergenic 40.0%
TACTAACGGTCTAGCTAAAG+AGG - chr2.1:44393921-44393940 MS.gene75277:intron 40.0%
TGATATGATGTAGCCTTCTC+CGG + chr2.1:44393583-44393602 None:intergenic 40.0%
! CTCCATGTGTCCCTTTTTAT+TGG + chr2.1:44393745-44393764 None:intergenic 40.0%
! CTTCTGGAGATCAGTGATAT+GGG + chr2.1:44394419-44394438 None:intergenic 40.0%
! GTTAGGGTACCTCCATTATA+TGG - chr2.1:44393370-44393389 MS.gene75277:CDS 40.0%
! TCTTCTGGAGATCAGTGATA+TGG + chr2.1:44394420-44394439 None:intergenic 40.0%
!!! TATTGATTTTCAGGCTCGAC+AGG - chr2.1:44394507-44394526 MS.gene75277:intron 40.0%
AAACCGGGTGGATATCTAAG+GGG + chr2.1:44394558-44394577 None:intergenic 45.0%
ACCGCTGAATCATGTTGAAG+CGG - chr2.1:44394288-44394307 MS.gene75277:intron 45.0%
AGAAACCGGGTGGATATCTA+AGG + chr2.1:44394560-44394579 None:intergenic 45.0%
AGCGGAAGCCTAGAAAAAGA+GGG - chr2.1:44394239-44394258 MS.gene75277:intron 45.0%
ATGGACAAGGCTTCTCTTCT+TGG - chr2.1:44394382-44394401 MS.gene75277:intron 45.0%
CCAAAGATAGTTGGGTCTAG+CGG - chr2.1:44394114-44394133 MS.gene75277:intron 45.0%
GAAACCGGGTGGATATCTAA+GGG + chr2.1:44394559-44394578 None:intergenic 45.0%
GGTTTGTCAAACACCAAGTG+TGG + chr2.1:44394001-44394020 None:intergenic 45.0%
TAACAATTCCAGAAACCGGG+TGG + chr2.1:44394570-44394589 None:intergenic 45.0%
TCCGCTTCAACATGATTCAG+CGG + chr2.1:44394292-44394311 None:intergenic 45.0%
TCTCACAACGTGGTGATTCA+AGG - chr2.1:44393950-44393969 MS.gene75277:intron 45.0%
TCTCCATCAGAACTCAAGGA+GGG - chr2.1:44394665-44394684 MS.gene75277:intron 45.0%
TGAATCATGTTGAAGCGGAG+AGG - chr2.1:44394293-44394312 MS.gene75277:intron 45.0%
TTAACTCGTGTCTTGCCTCT+TGG - chr2.1:44394211-44394230 MS.gene75277:intron 45.0%
TTAGATATCCACCCGGTTTC+TGG - chr2.1:44394559-44394578 MS.gene75277:intron 45.0%
TTCTCCATCAGAACTCAAGG+AGG - chr2.1:44394664-44394683 MS.gene75277:intron 45.0%
TTGAAGCGGAGAGGCAAAAA+CGG - chr2.1:44394302-44394321 MS.gene75277:intron 45.0%
! GGCTTTCTCCCTCTTTTTCT+AGG + chr2.1:44394250-44394269 None:intergenic 45.0%
CCGCTAGACCCAACTATCTT+TGG + chr2.1:44394117-44394136 None:intergenic 50.0%
CTACAAGTGGAGTGAAGCCA+TGG - chr2.1:44394065-44394084 MS.gene75277:intron 50.0%
CTCCATCAGAACTCAAGGAG+GGG - chr2.1:44394666-44394685 MS.gene75277:intron 50.0%
GAGCGGAAGCCTAGAAAAAG+AGG - chr2.1:44394238-44394257 MS.gene75277:intron 50.0%
GAGGTTAGACTCTCACAACG+TGG - chr2.1:44393940-44393959 MS.gene75277:intron 50.0%
GGCCAATGTTTCAACTTCCC+AGG - chr2.1:44394624-44394643 MS.gene75277:intron 50.0%
TATTGAGGGTTTGTCGGGCA+AGG + chr2.1:44394039-44394058 None:intergenic 50.0%
TGAAGCGGAGAGGCAAAAAC+GGG - chr2.1:44394303-44394322 MS.gene75277:intron 50.0%
! TTCCCCTCCTTGAGTTCTGA+TGG + chr2.1:44394671-44394690 None:intergenic 50.0%
AAAGCCTGCCAATGGACGAG+AGG - chr2.1:44394264-44394283 MS.gene75277:intron 55.0%
AGAGGGAGAAAGCCTGCCAA+TGG - chr2.1:44394256-44394275 MS.gene75277:intron 55.0%
CTGCCCCTTAGATATCCACC+CGG - chr2.1:44394552-44394571 MS.gene75277:intron 55.0%
GTCTGCAGCTTCGCTGAAGA+TGG - chr2.1:44393212-44393231 MS.gene75277:CDS 55.0%
TCTTGCCTCTTGGAGACGAG+CGG - chr2.1:44394221-44394240 MS.gene75277:intron 55.0%
TGCAGCTTCGCTGAAGATGG+CGG - chr2.1:44393215-44393234 MS.gene75277:CDS 55.0%
! CTGAAGATGGCGGTGGAAAG+GGG - chr2.1:44393225-44393244 MS.gene75277:CDS 55.0%
! GCTGAAGATGGCGGTGGAAA+GGG - chr2.1:44393224-44393243 MS.gene75277:CDS 55.0%
CAGCGGTTCCTCTCGTCCAT+TGG + chr2.1:44394275-44394294 None:intergenic 60.0%
GGTTCCTCTCGTCCATTGGC+AGG + chr2.1:44394271-44394290 None:intergenic 60.0%
! AGCTTCGCTGAAGATGGCGG+TGG - chr2.1:44393218-44393237 MS.gene75277:CDS 60.0%
! CGCTGAAGATGGCGGTGGAA+AGG - chr2.1:44393223-44393242 MS.gene75277:CDS 60.0%
GGCTTCCGCTCGTCTCCAAG+AGG + chr2.1:44394229-44394248 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 44393200 44394744 44393200 ID=MS.gene75277
chr2.1 mRNA 44393200 44394744 44393200 ID=MS.gene75277.t1;Parent=MS.gene75277
chr2.1 exon 44394698 44394744 44394698 ID=MS.gene75277.t1.exon1;Parent=MS.gene75277.t1
chr2.1 CDS 44394698 44394744 44394698 ID=cds.MS.gene75277.t1;Parent=MS.gene75277.t1
chr2.1 exon 44393200 44393755 44393200 ID=MS.gene75277.t1.exon2;Parent=MS.gene75277.t1
chr2.1 CDS 44393200 44393755 44393200 ID=cds.MS.gene75277.t1;Parent=MS.gene75277.t1
Gene Sequence

>MS.gene75277

GTTCGTTTTGTGGTCTGCAGCTTCGCTGAAGATGGCGGTGGAAAGGGGTAATAGTTCTAGTGTTCAGACTAGATAAGGTGAAAAAACTATATTGTTTGACTCGTATGTTTCACTGTGTTATTCTTGTTATACCAGAAAATAGTTGTACTCTTCATGTTCTGCATGTTTCTGTTAGGGTACCTCCATTATATGGTAATGTTGTCTGTTTGATTCTGATCATCTCTACATGTTTCATTTTGAAACCTCATGACATTTTATCCTTGTTAGCATTAATATGTCATTTCAACTCTTATCTATATTTATTAATGCCGGTAAAATGGCTACAATGAAGTTTCTATAAGAAATTGAATTGTGATGACAATGGTCCTTGAAAATTAAGTCCGGAGAAGGCTACATCATATCATATGAGTAAGAAAGGCATAAGGCTAGAGATCACATCTTTTAATTGGTTTATGCAAGCTACTTATATCTTCTTTTCTTAATTCTCGTATTTGAAGGACAAACACAATAAAAATGATAACTGGACAATTAACACTAGAGACCCAATAAAAAGGGACACATGGAGTGACAAGATAAACAAAGCAAAGGGTCTATGATAATAAGAATAGCAAATAGATGAAATTCATAGTCTTATTTATTTACAAAATATATAATAAAAATGTGTGTGGTGCACTTTCCTACAATAATATCTCATTGCTACCACTTAAAAGCAACTATCCGATACTAACGGTCTAGCTAAAGAGGTTAGACTCTCACAACGTGGTGATTCAAGGTTTGAATCTCGATTGAGATAAGTATCCACACTTGGTGTTTGACAAACCTTCAATAATATTACTCCTTGCCCGACAAACCCTCAATAATATTACTACAAGTGGAGTGAAGCCATGGAAACCAAATCAAAAAACAAAAATGCACCAAAGATAGTTGGGTCTAGCGGAATGAACTAAATTTTTATAATGTGAAAAATCAATCTCTAAATTTTGGCTAAGGAGTGTATTTCTATTTAGTGTCTTAACTCGTGTCTTGCCTCTTGGAGACGAGCGGAAGCCTAGAAAAAGAGGGAGAAAGCCTGCCAATGGACGAGAGGAACCGCTGAATCATGTTGAAGCGGAGAGGCAAAAACGGGAGAAGCTTAACCAGAGATTTTACGCCTTAAGAGCTGTTGTTCCTAACATTTCTAAAATGGACAAGGCTTCTCTTCTTGGTGATGCTATTACCCATATCACTGATCTCCAGAAGAAGATCAAGCTTATGGAAACTGAAAAAAACATGGCTGACAATAAGGGACAACAATTGCCGTTGCAAGATATTGATTTTCAGGCTCGACAGGATGATGCAGTTGTTAGAGTTAGCTGCCCCTTAGATATCCACCCGGTTTCTGGAATTGTTAAAGTGTTCAGAGAGCATCAAATTGTAGCACAAGAGGCCAATGTTTCAACTTCCCAGGATAAAGTAATTCATACATTCTCCATCAGAACTCAAGGAGGGGAAGCAGCTGCTATACAGTTGAAAGAAAAGCTTGAAGCATCTTTAGCCAAAAATTAA
Protein sequence

>MS.gene75277.t1

VRFVVCSFAEDGGGKGSVFLFSVLTRVLPLGDERKPRKRGRKPANGREEPLNHVEAERQKREKLNQRFYALRAVVPNISKMDKASLLGDAITHITDLQKKIKLMETEKNMADNKGQQLPLQDIDFQARQDDAVVRVSCPLDIHPVSGIVKVFREHQIVAQEANVSTSQDKVIHTFSIRTQGGEAAAIQLKEKLEASLAKN