AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene76276


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene76276.t1 MTR_1g111280 96.818 220 7 0 1 220 125 344 5.11e-161 449
MS.gene76276.t1 MTR_7g406880 55.066 227 90 6 1 218 126 349 6.00e-80 243
MS.gene76276.t1 MTR_4g050140 40.444 225 113 8 1 218 105 315 1.74e-41 143
MS.gene76276.t1 MTR_7g107040 39.013 223 119 7 1 218 106 316 9.45e-41 141
MS.gene76276.t1 MTR_1g102170 37.619 210 115 4 1 205 132 330 1.71e-37 133
MS.gene76276.t1 MTR_8g035520 38.462 221 121 6 2 219 117 325 4.56e-36 129
MS.gene76276.t1 MTR_6g464320 37.500 224 118 5 2 219 119 326 4.91e-36 129
MS.gene76276.t1 MTR_0007s0520 41.827 208 102 5 2 201 116 312 6.83e-36 129
MS.gene76276.t1 MTR_6g464510 36.161 224 122 5 1 219 120 327 3.40e-35 127
MS.gene76276.t1 MTR_6g464280 37.946 224 117 5 2 219 118 325 1.56e-34 125
MS.gene76276.t1 MTR_6g464120 38.693 199 106 4 2 195 120 307 2.16e-33 125
MS.gene76276.t1 MTR_8g035540 36.818 220 124 6 2 218 109 316 1.04e-32 120
MS.gene76276.t1 MTR_1g102160 33.186 226 128 5 1 218 115 325 1.40e-32 120
MS.gene76276.t1 MTR_1g089310 39.000 200 101 6 1 197 142 323 2.14e-31 117
MS.gene76276.t1 MTR_1g089310 39.000 200 101 6 1 197 158 339 2.98e-31 117
MS.gene76276.t1 MTR_4g086510 34.091 220 129 7 1 218 112 317 6.33e-31 115
MS.gene76276.t1 MTR_7g093950 34.685 222 123 6 1 218 143 346 5.17e-30 114
MS.gene76276.t1 MTR_3g012440 37.436 195 108 6 6 196 130 314 1.09e-29 113
MS.gene76276.t1 MTR_1g082210 34.685 222 118 8 1 218 142 340 4.84e-29 111
MS.gene76276.t1 MTR_1g012430 38.122 181 98 6 1 179 102 270 5.86e-29 110
MS.gene76276.t1 MTR_3g101420 34.545 220 117 8 1 207 107 312 7.92e-29 110
MS.gene76276.t1 MTR_6g065120 37.443 219 115 9 1 214 134 335 1.70e-28 110
MS.gene76276.t1 MTR_7g056207 33.929 224 128 9 1 220 144 351 3.94e-28 109
MS.gene76276.t1 MTR_8g035500 35.407 209 121 7 1 205 111 309 3.95e-28 108
MS.gene76276.t1 MTR_6g065400 35.321 218 121 8 1 214 132 333 1.60e-27 107
MS.gene76276.t1 MTR_6g065270 35.484 217 122 8 1 214 134 335 2.17e-27 107
MS.gene76276.t1 MTR_6g065370 34.404 218 123 8 1 214 132 333 3.01e-27 106
MS.gene76276.t1 MTR_6g065430 37.563 197 105 7 1 194 126 307 3.54e-27 106
MS.gene76276.t1 MTR_1g105020 30.556 216 138 6 1 211 110 318 7.58e-26 102
MS.gene76276.t1 MTR_6g065110 33.790 219 127 8 1 216 133 336 1.76e-24 99.4
MS.gene76276.t1 MTR_4g086480 33.000 200 117 4 1 195 114 301 2.87e-24 98.2
MS.gene76276.t1 MTR_4g086470 33.520 179 107 3 1 179 113 279 2.43e-23 95.9
MS.gene76276.t1 MTR_0007s0480 41.875 160 82 4 50 201 2 158 3.54e-23 92.0
MS.gene76276.t1 MTR_6g065340 34.259 216 110 8 1 214 143 328 4.08e-22 92.8
MS.gene76276.t1 MTR_8g040860 30.942 223 135 8 1 215 118 329 7.30e-21 89.4
MS.gene76276.t1 MTR_3g450680 32.432 222 129 10 1 212 118 328 7.33e-21 89.4
MS.gene76276.t1 MTR_5g005850 32.039 206 121 9 1 197 115 310 1.02e-20 89.0
MS.gene76276.t1 MTR_7g051020 30.093 216 139 8 1 211 113 321 1.32e-19 85.9
MS.gene76276.t1 MTR_1g104930 31.868 182 114 6 1 179 110 284 1.98e-18 82.4
MS.gene76276.t1 MTR_4g086540 29.717 212 114 8 1 209 119 298 4.15e-18 81.6
MS.gene76276.t1 MTR_6g022710 29.231 260 131 9 1 220 197 443 1.55e-17 80.9
MS.gene76276.t1 MTR_1g104933 31.892 185 106 7 1 179 108 278 2.92e-16 76.3
MS.gene76276.t1 MTR_5g005920 28.351 194 121 6 2 188 119 301 3.86e-16 76.3
MS.gene76276.t1 MTR_5g005900 30.481 187 111 8 1 178 111 287 1.86e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene76276.t1 AT5G16080 54.128 218 95 3 1 218 129 341 1.62e-74 229
MS.gene76276.t1 AT1G68620 47.248 218 101 4 1 218 126 329 3.61e-63 199
MS.gene76276.t1 AT5G62180 39.130 207 108 5 1 201 117 311 5.64e-37 132
MS.gene76276.t1 AT3G63010 39.801 201 98 7 1 197 143 324 5.13e-35 127
MS.gene76276.t1 AT2G45610 35.586 222 127 6 1 219 116 324 1.32e-34 125
MS.gene76276.t1 AT3G05120 35.000 220 120 6 1 218 143 341 5.43e-33 122
MS.gene76276.t1 AT5G06570 38.500 200 109 7 1 194 128 319 9.70e-32 118
MS.gene76276.t1 AT5G06570 38.500 200 109 7 1 194 128 319 9.70e-32 118
MS.gene76276.t1 AT5G06570 37.879 198 113 6 1 194 113 304 1.04e-31 118
MS.gene76276.t1 AT5G06570 37.879 198 113 6 1 194 113 304 1.04e-31 118
MS.gene76276.t1 AT5G27320 36.199 221 116 8 1 218 141 339 3.81e-31 117
MS.gene76276.t1 AT2G45600 34.081 223 132 6 1 218 103 315 3.86e-29 111
MS.gene76276.t1 AT5G23530 35.268 224 124 8 1 219 124 331 7.85e-25 100
MS.gene76276.t1 AT1G19190 32.243 214 125 8 1 209 109 307 9.95e-25 99.4
MS.gene76276.t1 AT3G48700 30.732 205 124 7 1 196 111 306 7.15e-23 94.7
MS.gene76276.t1 AT2G03550 30.275 218 131 8 1 212 105 307 1.63e-20 88.2
MS.gene76276.t1 AT3G48690 32.065 184 111 8 1 179 108 282 2.39e-19 85.1
MS.gene76276.t1 AT1G47480 29.851 201 119 6 1 197 109 291 5.35e-19 84.0
MS.gene76276.t1 AT5G14310 30.435 230 123 7 1 197 188 413 2.12e-18 83.2
MS.gene76276.t1 AT1G49650 28.729 181 116 6 1 179 165 334 2.23e-17 80.1
MS.gene76276.t1 AT1G49660 39.423 104 45 3 1 98 109 200 2.41e-17 79.3
MS.gene76276.t1 AT1G49640 38.776 98 48 4 1 98 111 196 3.50e-15 73.2

Find 42 sgRNAs with CRISPR-Local

Find 50 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATAAGACCCTTTAAATTTAA+AGG 0.113115 1.1:+78571467 None:intergenic
TCTAAGGCCTTTAAATTTAA+AGG 0.122353 1.1:-78571474 MS.gene76276:CDS
GTGTAATCCATTGGTGAAAA+TGG 0.278139 1.1:-78571297 MS.gene76276:CDS
TAGCAGCTTCAGATACTTAC+TGG 0.285298 1.1:-78571361 MS.gene76276:CDS
TTCACATGAATCTATCCTTT+TGG 0.311453 1.1:+78571505 None:intergenic
CCTGAGATTTACTTAGAATC+TGG 0.322976 1.1:+78571126 None:intergenic
GTTGAATGTGAAGTGTTTAA+AGG 0.324790 1.1:-78571164 MS.gene76276:CDS
CTAAGGCCTTTAAATTTAAA+GGG 0.355632 1.1:-78571473 MS.gene76276:CDS
GCTACTGATGATGCCTATAT+TGG 0.378056 1.1:-78571264 MS.gene76276:CDS
CATGTGAAGGTGCATTTCTA+AGG 0.401620 1.1:-78571490 MS.gene76276:CDS
AAATGCATGGAACAATTATC+TGG 0.405311 1.1:-78571398 MS.gene76276:CDS
TCAGATATACACACCAATAT+AGG 0.414402 1.1:+78571251 None:intergenic
GATAAGAGTTGTTCAAATGA+TGG 0.417122 1.1:-78571096 MS.gene76276:CDS
TGTGATGCTTTAGGAAGAAC+AGG 0.432736 1.1:-78571194 MS.gene76276:CDS
TGGCGATTGGCATTACCATA+TGG 0.452089 1.1:-78571341 MS.gene76276:CDS
CTACCAGCACCTTATGATGA+TGG 0.461625 1.1:-78571671 MS.gene76276:CDS
TGTGAAGTGTTTAAAGGTGT+TGG 0.470256 1.1:-78571158 MS.gene76276:CDS
CCAGATTCTAAGTAAATCTC+AGG 0.474814 1.1:-78571126 MS.gene76276:CDS
TTCAGATACTTACTGGCGAT+TGG 0.489907 1.1:-78571354 MS.gene76276:CDS
CTTTAGGAAGAACAGGTACA+AGG 0.492026 1.1:-78571187 MS.gene76276:CDS
TGATCACGATCTTCACCATA+TGG 0.493029 1.1:+78571326 None:intergenic
ATCTGAGATGGATATATTGA+AGG 0.500971 1.1:-78571234 MS.gene76276:CDS
TATATTGAAGGATAGGAACT+TGG 0.516422 1.1:-78571222 MS.gene76276:CDS
AGATGGATATATTGAAGGAT+AGG 0.521304 1.1:-78571229 MS.gene76276:CDS
GCATTTAAACCATCATCATA+AGG 0.523827 1.1:+78571662 None:intergenic
TCATCATAAGGTGCTGGTAG+AGG 0.523884 1.1:+78571674 None:intergenic
TAGCTTATAATGTTGCCAAA+AGG 0.527439 1.1:-78571520 MS.gene76276:CDS
GGATTGTGTCAAATCATTCA+TGG 0.528865 1.1:-78571075 MS.gene76276:CDS
TTCTTAGGAGGTGATAGTGC+AGG 0.529844 1.1:-78571551 MS.gene76276:CDS
AAACCATCATCATAAGGTGC+TGG 0.546605 1.1:+78571668 None:intergenic
ATGAGAGTAGTGAATTTGAA+TGG 0.584788 1.1:-78571604 MS.gene76276:CDS
AACATTATCAGAGAAATGCA+TGG 0.593132 1.1:-78571411 MS.gene76276:CDS
AAAAGGATAGATTCATGTGA+AGG 0.595074 1.1:-78571503 MS.gene76276:CDS
TTCACCAATGGATTACACCA+TGG 0.598695 1.1:+78571302 None:intergenic
ATTGGTGTGTATATCTGAGA+TGG 0.604384 1.1:-78571246 MS.gene76276:CDS
TCATCCATGGTGTAATCCAT+TGG 0.611826 1.1:-78571306 MS.gene76276:CDS
TAATCCATTGGTGAAAATGG+AGG 0.623717 1.1:-78571294 MS.gene76276:CDS
GTGAAGATCGTGATCATCCA+TGG 0.631742 1.1:-78571319 MS.gene76276:CDS
TTTAGGAAGAACAGGTACAA+GGG 0.650756 1.1:-78571186 MS.gene76276:CDS
TTAGGAGGTGATAGTGCAGG+TGG 0.653434 1.1:-78571548 MS.gene76276:CDS
AGAGTAGTGAATTTGAATGG+TGG 0.668440 1.1:-78571601 MS.gene76276:CDS
ATGGTTTAAATGCACTAATG+TGG 0.680277 1.1:-78571652 MS.gene76276:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAGACCCTTTAAATTTAA+AGG + chr1.1:78571310-78571329 None:intergenic 20.0%
!!! ATTTTGATACAACCATTTTT+TGG - chr1.1:78571328-78571347 MS.gene76276:CDS 20.0%
! CTAAGGCCTTTAAATTTAAA+GGG - chr1.1:78571301-78571320 MS.gene76276:CDS 25.0%
!! AACTTTTCTAATGTCTTCTT+AGG - chr1.1:78571208-78571227 MS.gene76276:CDS 25.0%
!! TCTAAGGCCTTTAAATTTAA+AGG - chr1.1:78571300-78571319 MS.gene76276:CDS 25.0%
AAAAGGATAGATTCATGTGA+AGG - chr1.1:78571271-78571290 MS.gene76276:CDS 30.0%
AAATGCATGGAACAATTATC+TGG - chr1.1:78571376-78571395 MS.gene76276:CDS 30.0%
AACATTATCAGAGAAATGCA+TGG - chr1.1:78571363-78571382 MS.gene76276:CDS 30.0%
AGATGGATATATTGAAGGAT+AGG - chr1.1:78571545-78571564 MS.gene76276:CDS 30.0%
ATGAGAGTAGTGAATTTGAA+TGG - chr1.1:78571170-78571189 MS.gene76276:CDS 30.0%
ATGGTTTAAATGCACTAATG+TGG - chr1.1:78571122-78571141 MS.gene76276:CDS 30.0%
GATAAGAGTTGTTCAAATGA+TGG - chr1.1:78571678-78571697 MS.gene76276:CDS 30.0%
GCATTTAAACCATCATCATA+AGG + chr1.1:78571115-78571134 None:intergenic 30.0%
TAGCTTATAATGTTGCCAAA+AGG - chr1.1:78571254-78571273 MS.gene76276:CDS 30.0%
TATATTGAAGGATAGGAACT+TGG - chr1.1:78571552-78571571 MS.gene76276:CDS 30.0%
TCAGATATACACACCAATAT+AGG + chr1.1:78571526-78571545 None:intergenic 30.0%
! ATCTGAGATGGATATATTGA+AGG - chr1.1:78571540-78571559 MS.gene76276:CDS 30.0%
! CTTTCTTTTCCACCAAAAAA+TGG + chr1.1:78571343-78571362 None:intergenic 30.0%
! GTTGAATGTGAAGTGTTTAA+AGG - chr1.1:78571610-78571629 MS.gene76276:CDS 30.0%
! TTCACATGAATCTATCCTTT+TGG + chr1.1:78571272-78571291 None:intergenic 30.0%
! TTTTCTAATGTCTTCTTAGG+AGG - chr1.1:78571211-78571230 MS.gene76276:CDS 30.0%
!!! TTGATACAACCATTTTTTGG+TGG - chr1.1:78571331-78571350 MS.gene76276:CDS 30.0%
AGAGTAGTGAATTTGAATGG+TGG - chr1.1:78571173-78571192 MS.gene76276:CDS 35.0%
ATTGGTGTGTATATCTGAGA+TGG - chr1.1:78571528-78571547 MS.gene76276:CDS 35.0%
CCAGATTCTAAGTAAATCTC+AGG - chr1.1:78571648-78571667 MS.gene76276:CDS 35.0%
CCTGAGATTTACTTAGAATC+TGG + chr1.1:78571651-78571670 None:intergenic 35.0%
GGATTGTGTCAAATCATTCA+TGG - chr1.1:78571699-78571718 MS.gene76276:CDS 35.0%
GTGTAATCCATTGGTGAAAA+TGG - chr1.1:78571477-78571496 MS.gene76276:CDS 35.0%
TAATCCATTGGTGAAAATGG+AGG - chr1.1:78571480-78571499 MS.gene76276:CDS 35.0%
TTTAGGAAGAACAGGTACAA+GGG - chr1.1:78571588-78571607 MS.gene76276:CDS 35.0%
! AATTCCTCCATTTTCACCAA+TGG + chr1.1:78571487-78571506 None:intergenic 35.0%
! TGTGAAGTGTTTAAAGGTGT+TGG - chr1.1:78571616-78571635 MS.gene76276:CDS 35.0%
!! TTGGAGTTTTGTGATGCTTT+AGG - chr1.1:78571571-78571590 MS.gene76276:CDS 35.0%
AAACCATCATCATAAGGTGC+TGG + chr1.1:78571109-78571128 None:intergenic 40.0%
TAGCAGCTTCAGATACTTAC+TGG - chr1.1:78571413-78571432 MS.gene76276:CDS 40.0%
TCATCCATGGTGTAATCCAT+TGG - chr1.1:78571468-78571487 MS.gene76276:CDS 40.0%
TGATCACGATCTTCACCATA+TGG + chr1.1:78571451-78571470 None:intergenic 40.0%
TTCAGATACTTACTGGCGAT+TGG - chr1.1:78571420-78571439 MS.gene76276:CDS 40.0%
! CATGTGAAGGTGCATTTCTA+AGG - chr1.1:78571284-78571303 MS.gene76276:CDS 40.0%
! CTTTAGGAAGAACAGGTACA+AGG - chr1.1:78571587-78571606 MS.gene76276:CDS 40.0%
! GCTACTGATGATGCCTATAT+TGG - chr1.1:78571510-78571529 MS.gene76276:CDS 40.0%
!! TGTGATGCTTTAGGAAGAAC+AGG - chr1.1:78571580-78571599 MS.gene76276:CDS 40.0%
!! TTCACCAATGGATTACACCA+TGG + chr1.1:78571475-78571494 None:intergenic 40.0%
CTACCAGCACCTTATGATGA+TGG - chr1.1:78571103-78571122 MS.gene76276:CDS 45.0%
GTGAAGATCGTGATCATCCA+TGG - chr1.1:78571455-78571474 MS.gene76276:CDS 45.0%
TGGCGATTGGCATTACCATA+TGG - chr1.1:78571433-78571452 MS.gene76276:CDS 45.0%
! TTCTTAGGAGGTGATAGTGC+AGG - chr1.1:78571223-78571242 MS.gene76276:CDS 45.0%
!! TCATCATAAGGTGCTGGTAG+AGG + chr1.1:78571103-78571122 None:intergenic 45.0%
! TTAGGAGGTGATAGTGCAGG+TGG - chr1.1:78571226-78571245 MS.gene76276:CDS 50.0%
!!! GGTGCTGGTAGAGGATTTTC+TGG + chr1.1:78571094-78571113 None:intergenic 50.0%


Chromosome Type Strat End Strand Name
chr1.1 gene 78571067 78571729 78571067 ID=MS.gene76276
chr1.1 mRNA 78571067 78571729 78571067 ID=MS.gene76276.t1;Parent=MS.gene76276
chr1.1 exon 78571067 78571729 78571067 ID=MS.gene76276.t1.exon1;Parent=MS.gene76276.t1
chr1.1 CDS 78571067 78571729 78571067 ID=cds.MS.gene76276.t1;Parent=MS.gene76276.t1
Gene Sequence

>MS.gene76276

ATGTCAGTTAACTATAGATTAGCACCAGAAAATCCTCTACCAGCACCTTATGATGATGGTTTAAATGCACTAATGTGGTTGAAAAAACAATTTTTATATCAAAATGAGAGTAGTGAATTTGAATGGTGGACTAAAAAATGCAACTTTTCTAATGTCTTCTTAGGAGGTGATAGTGCAGGTGGTAACATAGCTTATAATGTTGCCAAAAGGATAGATTCATGTGAAGGTGCATTTCTAAGGCCTTTAAATTTAAAGGGTCTTATTTTGATACAACCATTTTTTGGTGGAAAAGAAAGAACATTATCAGAGAAATGCATGGAACAATTATCTGGTTCTGCTTTGAATTTAGCAGCTTCAGATACTTACTGGCGATTGGCATTACCATATGGTGAAGATCGTGATCATCCATGGTGTAATCCATTGGTGAAAATGGAGGAATTGAAGCTACTGATGATGCCTATATTGGTGTGTATATCTGAGATGGATATATTGAAGGATAGGAACTTGGAGTTTTGTGATGCTTTAGGAAGAACAGGTACAAGGGTTGAATGTGAAGTGTTTAAAGGTGTTGGACATGCTTTCCAGATTCTAAGTAAATCTCAGGTCTCTAAGATAAGAGTTGTTCAAATGATGGATTGTGTCAAATCATTCATGGCTCTTTGA

Protein sequence

>MS.gene76276.t1

MSVNYRLAPENPLPAPYDDGLNALMWLKKQFLYQNESSEFEWWTKKCNFSNVFLGGDSAGGNIAYNVAKRIDSCEGAFLRPLNLKGLILIQPFFGGKERTLSEKCMEQLSGSALNLAASDTYWRLALPYGEDRDHPWCNPLVKMEELKLLMMPILVCISEMDILKDRNLEFCDALGRTGTRVECEVFKGVGHAFQILSKSQVSKIRVVQMMDCVKSFMAL