AlfalfaGEDB | MS.gene52621

M. sativa cultivar XinJiangDaYe / MS.gene52621

KEGG: K14493
GO: GO:0003674 GO:0003824 GO:0016787
PFAM: PF07859.16

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene52621.t1	XP_024638169.1	99.4	334	2	0	1	334	1	334	2.70E-195	691

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene52621.t1	Q940G6	77.9	330	72	1	4	333	15	343	1.9e-157	556.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene52621.t1	G7I7F8	99.1	333	3	0	2	334	13	345	1.6e-194	688.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049150	MS.gene52621	0.828033	1.13E-54	-1.69E-46
MS.gene049153	MS.gene52621	0.814846	1.25E-51	-1.69E-46
MS.gene049260	MS.gene52621	0.856518	3.05E-62	-1.69E-46
MS.gene049297	MS.gene52621	0.810418	1.16E-50	-1.69E-46
MS.gene049346	MS.gene52621	0.806274	8.91E-50	-1.69E-46
MS.gene049758	MS.gene52621	0.808996	2.35E-50	-1.69E-46
MS.gene050128	MS.gene52621	0.819238	1.29E-52	-1.69E-46
MS.gene050301	MS.gene52621	0.827944	1.18E-54	-1.69E-46
MS.gene050642	MS.gene52621	0.805898	1.07E-49	-1.69E-46
MS.gene051253	MS.gene52621	0.821108	4.81E-53	-1.69E-46
MS.gene051582	MS.gene52621	0.811667	6.24E-51	-1.69E-46
MS.gene051789	MS.gene52621	0.801064	1.07E-48	-1.69E-46
MS.gene051860	MS.gene52621	0.818827	1.60E-52	-1.69E-46
MS.gene052176	MS.gene52621	0.806413	8.33E-50	-1.69E-46
MS.gene052178	MS.gene52621	0.818574	1.83E-52	-1.69E-46
MS.gene053074	MS.gene52621	0.802492	5.47E-49	-1.69E-46
MS.gene053204	MS.gene52621	0.803138	4.02E-49	-1.69E-46
MS.gene053555	MS.gene52621	0.833135	6.35E-56	-1.69E-46
MS.gene053836	MS.gene52621	0.84152	4.52E-58	-1.69E-46
MS.gene053928	MS.gene52621	0.816904	4.35E-52	-1.69E-46
MS.gene054010	MS.gene52621	0.841629	4.23E-58	-1.69E-46
MS.gene054011	MS.gene52621	0.848102	7.59E-60	-1.69E-46
MS.gene054012	MS.gene52621	0.8522	5.40E-61	-1.69E-46
MS.gene054013	MS.gene52621	0.847284	1.27E-59	-1.69E-46
MS.gene054183	MS.gene52621	0.833428	5.36E-56	-1.69E-46
MS.gene054214	MS.gene52621	0.833327	5.68E-56	-1.69E-46
MS.gene054379	MS.gene52621	0.801374	9.28E-49	-1.69E-46
MS.gene054473	MS.gene52621	0.834701	2.57E-56	-1.69E-46
MS.gene054930	MS.gene52621	0.802006	6.89E-49	-1.69E-46
MS.gene054942	MS.gene52621	0.810162	1.32E-50	-1.69E-46
MS.gene054983	MS.gene52621	0.812176	4.84E-51	-1.69E-46
MS.gene055356	MS.gene52621	0.827312	1.68E-54	-1.69E-46
MS.gene055358	MS.gene52621	0.83088	2.29E-55	-1.69E-46
MS.gene055428	MS.gene52621	0.823805	1.14E-53	-1.69E-46
MS.gene055604	MS.gene52621	0.862208	5.96E-64	-1.69E-46
MS.gene056723	MS.gene52621	0.833073	6.57E-56	-1.69E-46
MS.gene056772	MS.gene52621	0.846547	2.03E-59	-1.69E-46
MS.gene056902	MS.gene52621	0.823935	1.06E-53	-1.69E-46
MS.gene05702	MS.gene52621	0.804176	2.45E-49	-1.69E-46
MS.gene057121	MS.gene52621	0.812962	3.26E-51	-1.69E-46
MS.gene057469	MS.gene52621	0.827529	1.49E-54	-1.69E-46
MS.gene057639	MS.gene52621	0.833091	6.51E-56	-1.69E-46
MS.gene057752	MS.gene52621	0.816425	5.57E-52	-1.69E-46
MS.gene058002	MS.gene52621	0.832614	8.55E-56	-1.69E-46
MS.gene05811	MS.gene52621	0.813446	2.55E-51	-1.69E-46
MS.gene058142	MS.gene52621	0.849572	2.97E-60	-1.69E-46
MS.gene058749	MS.gene52621	0.806529	7.87E-50	-1.69E-46
MS.gene058833	MS.gene52621	0.800435	1.44E-48	-1.69E-46
MS.gene059006	MS.gene52621	0.821668	3.57E-53	-1.69E-46
MS.gene059011	MS.gene52621	0.803515	3.36E-49	-1.69E-46
MS.gene05907	MS.gene52621	0.801577	8.43E-49	-1.69E-46
MS.gene059128	MS.gene52621	0.807906	4.02E-50	-1.69E-46
MS.gene059247	MS.gene52621	0.824582	7.47E-54	-1.69E-46
MS.gene059420	MS.gene52621	0.801283	9.69E-49	-1.69E-46
MS.gene05984	MS.gene52621	0.817923	2.56E-52	-1.69E-46
MS.gene059894	MS.gene52621	0.800652	1.30E-48	-1.69E-46
MS.gene061049	MS.gene52621	0.829169	5.98E-55	-1.69E-46
MS.gene061112	MS.gene52621	0.80158	8.42E-49	-1.69E-46
MS.gene061114	MS.gene52621	-0.809495	1.84E-50	-1.69E-46
MS.gene061265	MS.gene52621	0.803196	3.91E-49	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene068460	MS.gene52621	PPI
MS.gene068472	MS.gene52621	PPI
MS.gene04666	MS.gene52621	PPI
MS.gene52621	MS.gene21543	PPI
MS.gene055956	MS.gene52621	PPI
MS.gene93630	MS.gene52621	PPI
MS.gene52621	MS.gene029377	PPI
MS.gene069338	MS.gene52621	PPI
MS.gene062670	MS.gene52621	PPI
MS.gene97877	MS.gene52621	PPI
MS.gene76276	MS.gene52621	PPI
MS.gene07155	MS.gene52621	PPI
MS.gene018032	MS.gene52621	PPI
MS.gene93628	MS.gene52621	PPI
MS.gene042787	MS.gene52621	PPI
MS.gene52621	MS.gene005657	PPI
MS.gene52621	MS.gene068477	PPI
MS.gene065106	MS.gene52621	PPI
MS.gene54227	MS.gene52621	PPI
MS.gene068475	MS.gene52621	PPI
MS.gene042778	MS.gene52621	PPI
MS.gene042786	MS.gene52621	PPI
MS.gene062660	MS.gene52621	PPI
MS.gene062658	MS.gene52621	PPI
MS.gene52621	MS.gene042779	PPI
MS.gene062669	MS.gene52621	PPI
MS.gene062655	MS.gene52621	PPI
MS.gene52704	MS.gene52621	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene52621.t1	MTR_1g082210	99.099	333	3	0	2	334	13	345	0.0	682
MS.gene52621.t1	MTR_1g089310	72.072	333	91	2	2	333	13	344	0.0	512
MS.gene52621.t1	MTR_1g089310	72.072	333	91	2	2	333	29	360	0.0	512
MS.gene52621.t1	MTR_7g093950	71.681	339	88	6	2	333	13	350	6.59e-179	499
MS.gene52621.t1	MTR_6g065400	40.909	330	188	2	4	330	9	334	1.66e-83	256
MS.gene52621.t1	MTR_6g065110	39.583	336	196	2	2	334	8	339	1.91e-79	246
MS.gene52621.t1	MTR_6g065120	40.836	311	176	3	24	330	30	336	5.74e-79	244
MS.gene52621.t1	MTR_6g065370	39.091	330	194	2	4	330	9	334	3.30e-77	240
MS.gene52621.t1	MTR_6g065270	35.714	336	210	2	1	330	1	336	5.25e-74	232
MS.gene52621.t1	MTR_6g065430	38.065	310	176	3	25	329	29	327	5.28e-67	214
MS.gene52621.t1	MTR_6g065340	35.045	331	189	4	6	330	19	329	8.82e-63	203
MS.gene52621.t1	MTR_4g050140	34.843	287	160	5	56	329	43	315	1.02e-46	160
MS.gene52621.t1	MTR_7g406880	32.468	308	170	8	50	330	54	350	2.27e-41	147
MS.gene52621.t1	MTR_1g111280	31.310	313	175	8	42	332	51	345	1.05e-38	140
MS.gene52621.t1	MTR_7g107040	31.142	289	167	7	56	331	45	314	8.25e-38	137
MS.gene52621.t1	MTR_3g101420	29.373	303	169	7	42	333	32	300	8.27e-37	134
MS.gene52621.t1	MTR_8g035540	29.573	328	191	9	27	334	14	321	4.91e-36	133
MS.gene52621.t1	MTR_1g102170	33.223	301	175	9	21	311	37	321	1.02e-35	132
MS.gene52621.t1	MTR_1g012430	33.212	274	158	7	27	294	16	270	2.67e-35	130
MS.gene52621.t1	MTR_7g056207	31.046	306	182	8	42	334	65	354	3.23e-35	131
MS.gene52621.t1	MTR_8g035520	30.462	325	184	9	26	331	25	326	1.80e-34	129
MS.gene52621.t1	MTR_8g035500	31.613	310	178	10	14	310	8	296	8.11e-34	127
MS.gene52621.t1	MTR_6g464120	33.884	242	134	7	21	253	23	247	1.03e-33	130
MS.gene52621.t1	MTR_6g464510	30.065	306	177	9	21	309	22	307	1.45e-33	126
MS.gene52621.t1	MTR_1g102160	32.270	282	163	7	27	294	24	291	1.51e-33	126
MS.gene52621.t1	MTR_3g012440	32.627	236	148	3	86	311	80	314	4.42e-33	125
MS.gene52621.t1	MTR_4g086510	30.132	302	182	8	42	330	33	318	5.30e-33	124
MS.gene52621.t1	MTR_0007s0520	32.883	222	127	5	42	254	38	246	3.15e-32	122
MS.gene52621.t1	MTR_6g464320	30.033	303	186	9	20	308	15	305	3.84e-31	120
MS.gene52621.t1	MTR_6g022710	31.660	259	136	4	94	312	160	417	4.11e-30	119
MS.gene52621.t1	MTR_4g086480	27.575	301	186	10	42	329	37	318	8.89e-30	116
MS.gene52621.t1	MTR_6g464280	30.769	234	143	6	26	252	23	244	1.43e-29	115
MS.gene52621.t1	MTR_3g450680	28.221	326	188	11	27	329	29	331	2.95e-29	115
MS.gene52621.t1	MTR_4g086470	29.658	263	156	8	42	294	36	279	1.01e-27	110
MS.gene52621.t1	MTR_4g086540	28.617	311	172	11	27	328	31	300	1.06e-27	110
MS.gene52621.t1	MTR_1g105020	28.956	297	169	9	27	303	21	295	5.18e-26	105
MS.gene52621.t1	MTR_5g005920	27.742	310	189	10	14	304	9	302	1.14e-25	105
MS.gene52621.t1	MTR_7g051020	31.343	268	149	8	54	304	48	297	8.85e-25	102
MS.gene52621.t1	MTR_5g005900	26.403	303	181	9	12	296	12	290	1.10e-24	102
MS.gene52621.t1	MTR_8g040860	26.230	305	190	9	43	330	43	329	1.13e-24	102
MS.gene52621.t1	MTR_5g005850	26.780	295	177	9	27	303	26	299	6.79e-24	100
MS.gene52621.t1	MTR_1g104933	31.641	256	137	8	54	294	46	278	1.35e-23	99.4
MS.gene52621.t1	MTR_1g104930	31.359	287	151	11	27	294	25	284	2.03e-23	99.0
MS.gene52621.t1	MTR_6g065470	35.461	141	72	4	22	158	37	162	1.64e-15	73.6
MS.gene52621.t1	MTR_0007s0480	37.079	89	49	1	172	253	3	91	5.23e-12	63.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene52621.t1	AT5G27320	77.410	332	74	1	2	333	13	343	0.0	555
MS.gene52621.t1	AT3G05120	76.276	333	78	1	2	333	13	345	0.0	546
MS.gene52621.t1	AT3G63010	69.880	332	97	3	2	331	13	343	1.07e-177	496
MS.gene52621.t1	AT5G23530	36.499	337	187	6	2	330	10	327	2.44e-68	217
MS.gene52621.t1	AT1G68620	35.887	248	138	5	51	291	57	290	1.18e-41	148
MS.gene52621.t1	AT5G62180	33.449	287	172	7	21	297	15	292	1.34e-41	147
MS.gene52621.t1	AT5G06570	33.651	315	170	8	36	331	48	342	1.07e-40	145
MS.gene52621.t1	AT5G06570	33.651	315	170	8	36	331	48	342	1.07e-40	145
MS.gene52621.t1	AT5G06570	33.651	315	170	8	36	331	33	327	1.08e-40	145
MS.gene52621.t1	AT5G06570	33.651	315	170	8	36	331	33	327	1.08e-40	145
MS.gene52621.t1	AT5G16080	32.000	300	166	8	42	325	53	330	2.24e-37	137
MS.gene52621.t1	AT1G47480	30.420	286	174	6	22	303	16	280	3.06e-36	133
MS.gene52621.t1	AT1G19190	32.039	309	172	11	42	333	30	317	2.13e-34	128
MS.gene52621.t1	AT2G03550	31.230	317	180	10	28	330	19	311	2.32e-34	128
MS.gene52621.t1	AT2G45600	33.585	265	144	7	54	301	39	288	6.88e-34	127
MS.gene52621.t1	AT2G45610	31.757	296	172	9	21	301	16	296	9.61e-34	127
MS.gene52621.t1	AT3G48700	33.231	325	163	13	39	332	27	328	1.38e-33	126
MS.gene52621.t1	AT3G48690	32.234	273	151	7	39	294	27	282	3.17e-33	125
MS.gene52621.t1	AT5G14310	31.439	264	135	4	94	312	151	413	1.40e-32	126
MS.gene52621.t1	AT3G27320	33.080	263	131	5	94	312	134	395	1.95e-30	120
MS.gene52621.t1	AT3G27320	33.080	263	131	5	94	312	166	427	2.94e-30	120
MS.gene52621.t1	AT1G49650	30.798	263	158	7	42	294	86	334	2.68e-29	115
MS.gene52621.t1	AT1G49640	29.020	255	165	5	43	293	32	274	9.83e-27	107
MS.gene52621.t1	AT1G49660	29.502	261	161	5	42	292	30	277	1.12e-26	107

Find 91 sgRNAs with CRISPR-Local

Find 101 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ACAGAGAAATGCTCATTATT+TGG	0.229509	1.1:-60844542	None:intergenic
CCATCAAGGCTTCGACCTTT+TGG	0.246635	1.1:-60844371	None:intergenic
TTGTCGTCTCGGTGAATTAT+AGG	0.247260	1.1:+60843991	MS.gene52621:CDS
ATGGGTGGAAAGCTGTTAAA+TGG	0.260029	1.1:+60844051	MS.gene52621:CDS
AAGAGTCTTGTGGTGGTTGC+TGG	0.278599	1.1:+60844407	MS.gene52621:CDS
TGATCTCTGTCTTCCCCTTC+AGG	0.298031	1.1:-60844326	None:intergenic
TGCTGGTTTGGATCTTGTTC+AGG	0.302371	1.1:+60844424	MS.gene52621:CDS
ATTGTATGCTAACTTGAAAT+TGG	0.306162	1.1:-60843646	None:intergenic
TCTTGTGGTGGTTGCTGGTT+TGG	0.332790	1.1:+60844412	MS.gene52621:CDS
TCCAGCTTTCTCAAGTCCTT+TGG	0.334783	1.1:-60844465	None:intergenic
TCCACAGGATTCGCATTTGC+TGG	0.346221	1.1:-60843732	None:intergenic
AGCATCGGACTCTGGGATTC+CGG	0.346427	1.1:+60844178	MS.gene52621:CDS
GCAACCACCACAAGACTCTT+AGG	0.357754	1.1:-60844404	None:intergenic
ATAGGCGTGCGCCTGAAAAT+AGG	0.363489	1.1:+60844009	MS.gene52621:CDS
CTTGTTCAGGATTGGCAATT+GGG	0.363782	1.1:+60844437	MS.gene52621:CDS
TCTTGTTCAGGATTGGCAAT+TGG	0.368427	1.1:+60844436	MS.gene52621:CDS
ATACTGCTAAACCCTTTGTT+TGG	0.396562	1.1:+60844212	MS.gene52621:CDS
GACTCTGGGATTCCGGTGTT+GGG	0.401673	1.1:+60844185	MS.gene52621:CDS
TTCTCGAGCAAGCCACGGTT+GGG	0.404625	1.1:+60844507	MS.gene52621:CDS
GGACTCTGGGATTCCGGTGT+TGG	0.405337	1.1:+60844184	MS.gene52621:CDS
GGAAGAACCTCAGCAGTAAC+AGG	0.409700	1.1:-60843867	None:intergenic
GTTTGGATCTTGTTCAGGAT+TGG	0.415765	1.1:+60844429	MS.gene52621:CDS
GAATCCGAAAAGCGTCTTGA+TGG	0.417426	1.1:+60844251	MS.gene52621:CDS
TGAAAGATAGAGACTGGTAT+TGG	0.418998	1.1:+60844291	MS.gene52621:CDS
TGTTGTTCTTCTCCCTCAAC+AGG	0.421393	1.1:-60843819	None:intergenic
CATCACGCGTGTAACCCTTT+TGG	0.421489	1.1:+60844347	MS.gene52621:CDS
CGAATCTACCGTCCTGTTGA+GGG	0.426280	1.1:+60843807	MS.gene52621:CDS
CCAAAAGGTCGAAGCCTTGA+TGG	0.429491	1.1:+60844371	MS.gene52621:CDS
TGCTAAACCCTTTGTTTGGC+GGG	0.429653	1.1:+60844216	MS.gene52621:CDS
GCATTGAAAGCATCGGACTC+TGG	0.434852	1.1:+60844170	MS.gene52621:CDS
ATCAAAACCCACATGTTCAA+TGG	0.451446	1.1:-60843621	None:intergenic
CCTGTTGTCATGTTCTTCCA+CGG	0.459630	1.1:+60843888	MS.gene52621:CDS
TATCCTTGCGCTTATGAAGA+TGG	0.468068	1.1:+60844032	MS.gene52621:CDS
TTTCTCGAGCAAGCCACGGT+TGG	0.476045	1.1:+60844506	MS.gene52621:CDS
TGGGTGGAAAGCTGTTAAAT+GGG	0.477103	1.1:+60844052	MS.gene52621:CDS
CTACATGGTTGGTGATAGCT+CGG	0.486021	1.1:+60844124	MS.gene52621:CDS
TTGTAATGCTGTTGTCGTCT+CGG	0.487442	1.1:+60843980	MS.gene52621:CDS
CTTTCTTCCCCGCCAAACAA+AGG	0.487810	1.1:-60844224	None:intergenic
CTGCTAAACCCTTTGTTTGG+CGG	0.491910	1.1:+60844215	MS.gene52621:CDS
GTAGATTCGAGTAAGGAGAT+TGG	0.497470	1.1:-60843793	None:intergenic
CATTGAAAGCATCGGACTCT+GGG	0.497517	1.1:+60844171	MS.gene52621:CDS
TGGAACTTTCAACCGTGACT+TGG	0.499887	1.1:+60843686	MS.gene52621:CDS
CCGTGGAAGAACATGACAAC+AGG	0.502089	1.1:-60843888	None:intergenic
GCCAAAGGACTTGAGAAAGC+TGG	0.509171	1.1:+60844464	MS.gene52621:CDS
TTTCTTCCCCGCCAAACAAA+GGG	0.509175	1.1:-60844223	None:intergenic
TGCCTTTCCTAAGAGTCTTG+TGG	0.514282	1.1:+60844397	MS.gene52621:CDS
AATCTTCTCCGTCGTCCTGA+TGG	0.518337	1.1:+60843666	MS.gene52621:CDS
CAAAAGGTCGAAGCCTTGAT+GGG	0.521272	1.1:+60844372	MS.gene52621:CDS
TAGATAAAATTTGATGCAGA+TGG	0.524069	1.1:+60843593	None:intergenic
TGATACATTGTGTCGTCGAT+TGG	0.529626	1.1:+60843950	MS.gene52621:CDS
AATGGGTTAACTCTAGAACT+TGG	0.530294	1.1:+60844069	MS.gene52621:CDS
TCGAATCTACCGTCCTGTTG+AGG	0.542489	1.1:+60843806	MS.gene52621:CDS
TCACGGTTGAAAGTTCCATC+AGG	0.545176	1.1:-60843681	None:intergenic
ACTTGGCAGAGTTTCTTGAT+CGG	0.546431	1.1:+60843703	MS.gene52621:CDS
ATCCTTGCGCTTATGAAGAT+GGG	0.546497	1.1:+60844033	MS.gene52621:CDS
AATCCGAAAAGCGTCTTGAT+GGG	0.557814	1.1:+60844252	MS.gene52621:CDS
TCAGAGTGAAAGATAGAGAC+TGG	0.557918	1.1:+60844285	MS.gene52621:CDS
AGCAGTATATTTCCCAACAC+CGG	0.572967	1.1:-60844197	None:intergenic
CAGGACGGTAGATTCGAGTA+AGG	0.573555	1.1:-60843800	None:intergenic
TAGGAAAGGCAACCCCATCA+AGG	0.574933	1.1:-60844385	None:intergenic
CACCCATCTTCATAAGCGCA+AGG	0.575641	1.1:-60844035	None:intergenic
AAGGTTCATATCTACATGGT+TGG	0.580511	1.1:+60844113	MS.gene52621:CDS
ATAAAATTTGATGCAGATGG+TGG	0.580628	1.1:+60843596	None:intergenic
TGAGCATTTCTCTGTTGTCA+TGG	0.580679	1.1:+60844550	MS.gene52621:CDS
CTTTCCTAAGAGTCTTGTGG+TGG	0.585467	1.1:+60844400	MS.gene52621:CDS
ATGGTTGGTGATAGCTCGGG+CGG	0.594503	1.1:+60844128	MS.gene52621:CDS
TACATGGTTGGTGATAGCTC+GGG	0.594607	1.1:+60844125	MS.gene52621:CDS
ACATTGTGTCGTCGATTGGT+TGG	0.596982	1.1:+60843954	MS.gene52621:CDS
CAGCAGTAACAGGCTTCTCA+AGG	0.598120	1.1:-60843857	None:intergenic
TGGTGGTTCCATTGAACATG+TGG	0.598890	1.1:+60843613	MS.gene52621:CDS
TGAGAAGCCTGTTACTGCTG+AGG	0.600623	1.1:+60843860	MS.gene52621:CDS
GCTATTTCTCGAGCAAGCCA+CGG	0.605537	1.1:+60844502	MS.gene52621:CDS
CCATGTTGCATTGAAAGCAT+CGG	0.608422	1.1:+60844163	MS.gene52621:CDS
GCTAAACCCTTTGTTTGGCG+GGG	0.608927	1.1:+60844217	MS.gene52621:CDS
AAAGAGAAAACACCATCCAC+AGG	0.610297	1.1:-60843747	None:intergenic
CAAGAAACTCTGCCAAGTCA+CGG	0.617005	1.1:-60843698	None:intergenic
TGAAAGTTCCATCAGGACGA+CGG	0.623383	1.1:-60843674	None:intergenic
TAGCAAGGTTCATATCTACA+TGG	0.626488	1.1:+60844109	MS.gene52621:CDS
GTTCTTCTCCCTCAACAGGA+CGG	0.631361	1.1:-60843815	None:intergenic
TGGCAATTGGGATATGCCAA+AGG	0.633591	1.1:+60844449	MS.gene52621:CDS
CTTGCGCTTATGAAGATGGG+TGG	0.634175	1.1:+60844036	MS.gene52621:CDS
TCCAGCAAATGCGAATCCTG+TGG	0.635598	1.1:+60843731	MS.gene52621:CDS
CACCACAAGACTCTTAGGAA+AGG	0.635838	1.1:-60844399	None:intergenic
CCGATGCTTTCAATGCAACA+TGG	0.638442	1.1:-60844163	None:intergenic
GGTGGTTCCATTGAACATGT+GGG	0.653078	1.1:+60843614	MS.gene52621:CDS
AAAAGGTCGAAGCCTTGATG+GGG	0.659766	1.1:+60844373	MS.gene52621:CDS
TGGTTGGTGATAGCTCGGGC+GGG	0.665839	1.1:+60844129	MS.gene52621:CDS
GTTGTCATGTTCTTCCACGG+TGG	0.676583	1.1:+60843891	MS.gene52621:CDS
GCAAATGCGAATCCTGTGGA+TGG	0.677631	1.1:+60843735	MS.gene52621:CDS
TAGCAAGTAAAACCCAACCG+TGG	0.774514	1.1:-60844519	None:intergenic
AATGTGCAAAACTTCCACCG+TGG	0.777602	1.1:-60843905	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	ATTGTATGCTAACTTGAAAT+TGG	-	chr1.1:60843649-60843668	None:intergenic	25.0%
!!	CTTTTGATGTTATTGTTGAT+CGG	+	chr1.1:60843766-60843785	MS.gene52621:CDS	25.0%
!!	TTTTGATGTTATTGTTGATC+GGG	+	chr1.1:60843767-60843786	MS.gene52621:CDS	25.0%
	ACAGAGAAATGCTCATTATT+TGG	-	chr1.1:60844545-60844564	None:intergenic	30.0%
	AAGGTTCATATCTACATGGT+TGG	+	chr1.1:60844113-60844132	MS.gene52621:CDS	35.0%
	ATACTGCTAAACCCTTTGTT+TGG	+	chr1.1:60844212-60844231	MS.gene52621:CDS	35.0%
	ATCAAAACCCACATGTTCAA+TGG	-	chr1.1:60843624-60843643	None:intergenic	35.0%
	GCAGAGTAAAAAAGATAGCA+AGG	+	chr1.1:60844094-60844113	MS.gene52621:CDS	35.0%
	TAGCAAGGTTCATATCTACA+TGG	+	chr1.1:60844109-60844128	MS.gene52621:CDS	35.0%
	TGAAAGATAGAGACTGGTAT+TGG	+	chr1.1:60844291-60844310	MS.gene52621:CDS	35.0%
!	TGGAAGTTTTGCACATTCTT+CGG	+	chr1.1:60843911-60843930	MS.gene52621:CDS	35.0%
!!	AATGGGTTAACTCTAGAACT+TGG	+	chr1.1:60844069-60844088	MS.gene52621:CDS	35.0%
	AAAGAGAAAACACCATCCAC+AGG	-	chr1.1:60843750-60843769	None:intergenic	40.0%
	ACTTGGCAGAGTTTCTTGAT+CGG	+	chr1.1:60843703-60843722	MS.gene52621:CDS	40.0%
	AGCAGTATATTTCCCAACAC+CGG	-	chr1.1:60844200-60844219	None:intergenic	40.0%
	ATCCTTGCGCTTATGAAGAT+GGG	+	chr1.1:60844033-60844052	MS.gene52621:CDS	40.0%
	ATGGGTGGAAAGCTGTTAAA+TGG	+	chr1.1:60844051-60844070	MS.gene52621:CDS	40.0%
	GTAGATTCGAGTAAGGAGAT+TGG	-	chr1.1:60843796-60843815	None:intergenic	40.0%
	GTTTGGATCTTGTTCAGGAT+TGG	+	chr1.1:60844429-60844448	MS.gene52621:CDS	40.0%
	TATCCTTGCGCTTATGAAGA+TGG	+	chr1.1:60844032-60844051	MS.gene52621:CDS	40.0%
	TCAGAGTGAAAGATAGAGAC+TGG	+	chr1.1:60844285-60844304	MS.gene52621:CDS	40.0%
	TGAGCATTTCTCTGTTGTCA+TGG	+	chr1.1:60844550-60844569	MS.gene52621:CDS	40.0%
	TGATACATTGTGTCGTCGAT+TGG	+	chr1.1:60843950-60843969	MS.gene52621:CDS	40.0%
	TGGGTGGAAAGCTGTTAAAT+GGG	+	chr1.1:60844052-60844071	MS.gene52621:CDS	40.0%
	TTGTAATGCTGTTGTCGTCT+CGG	+	chr1.1:60843980-60843999	MS.gene52621:CDS	40.0%
	TTGTCGTCTCGGTGAATTAT+AGG	+	chr1.1:60843991-60844010	MS.gene52621:CDS	40.0%
!	CCATGTTGCATTGAAAGCAT+CGG	+	chr1.1:60844163-60844182	MS.gene52621:CDS	40.0%
!	CTTGTTCAGGATTGGCAATT+GGG	+	chr1.1:60844437-60844456	MS.gene52621:CDS	40.0%
!	TCTTGTTCAGGATTGGCAAT+TGG	+	chr1.1:60844436-60844455	MS.gene52621:CDS	40.0%
!	TGTAACCCTTTTGGTCCAAA+AGG	+	chr1.1:60844356-60844375	MS.gene52621:CDS	40.0%
!	TTCGACCTTTTGGACCAAAA+GGG	-	chr1.1:60844364-60844383	None:intergenic	40.0%
!!	AATCCGAAAAGCGTCTTGAT+GGG	+	chr1.1:60844252-60844271	MS.gene52621:CDS	40.0%
	AAAAGGTCGAAGCCTTGATG+GGG	+	chr1.1:60844373-60844392	MS.gene52621:CDS	45.0%
	AATGTGCAAAACTTCCACCG+TGG	-	chr1.1:60843908-60843927	None:intergenic	45.0%
	CAAAAGGTCGAAGCCTTGAT+GGG	+	chr1.1:60844372-60844391	MS.gene52621:CDS	45.0%
	CAAGAAACTCTGCCAAGTCA+CGG	-	chr1.1:60843701-60843720	None:intergenic	45.0%
	CACCACAAGACTCTTAGGAA+AGG	-	chr1.1:60844402-60844421	None:intergenic	45.0%
	CCGATGCTTTCAATGCAACA+TGG	-	chr1.1:60844166-60844185	None:intergenic	45.0%
	CCTGTTGTCATGTTCTTCCA+CGG	+	chr1.1:60843888-60843907	MS.gene52621:CDS	45.0%
	GGTGGTTCCATTGAACATGT+GGG	+	chr1.1:60843614-60843633	MS.gene52621:CDS	45.0%
	TAGCAAGTAAAACCCAACCG+TGG	-	chr1.1:60844522-60844541	None:intergenic	45.0%
	TCACGGTTGAAAGTTCCATC+AGG	-	chr1.1:60843684-60843703	None:intergenic	45.0%
	TCCAGCTTTCTCAAGTCCTT+TGG	-	chr1.1:60844468-60844487	None:intergenic	45.0%
	TGAAAGTTCCATCAGGACGA+CGG	-	chr1.1:60843677-60843696	None:intergenic	45.0%
	TGCCTTTCCTAAGAGTCTTG+TGG	+	chr1.1:60844397-60844416	MS.gene52621:CDS	45.0%
	TGGAACTTTCAACCGTGACT+TGG	+	chr1.1:60843686-60843705	MS.gene52621:CDS	45.0%
	TGGCAATTGGGATATGCCAA+AGG	+	chr1.1:60844449-60844468	MS.gene52621:CDS	45.0%
	TGGTGGTTCCATTGAACATG+TGG	+	chr1.1:60843613-60843632	MS.gene52621:CDS	45.0%
	TGTTGTTCTTCTCCCTCAAC+AGG	-	chr1.1:60843822-60843841	None:intergenic	45.0%
	TTTCTTCCCCGCCAAACAAA+GGG	-	chr1.1:60844226-60844245	None:intergenic	45.0%
!	ACATTGTGTCGTCGATTGGT+TGG	+	chr1.1:60843954-60843973	MS.gene52621:CDS	45.0%
!	CTGCTAAACCCTTTGTTTGG+CGG	+	chr1.1:60844215-60844234	MS.gene52621:CDS	45.0%
!	CTTCGACCTTTTGGACCAAA+AGG	-	chr1.1:60844365-60844384	None:intergenic	45.0%
!	CTTTCCTAAGAGTCTTGTGG+TGG	+	chr1.1:60844400-60844419	MS.gene52621:CDS	45.0%
!	GAGAGCTTTTCTTCCTGAAG+GGG	+	chr1.1:60844313-60844332	MS.gene52621:CDS	45.0%
!	GCGCAAGGATACCTATTTTC+AGG	-	chr1.1:60844023-60844042	None:intergenic	45.0%
!	GGAGAGCTTTTCTTCCTGAA+GGG	+	chr1.1:60844312-60844331	MS.gene52621:CDS	45.0%
!	TCTCCCATCAAGACGCTTTT+CGG	-	chr1.1:60844258-60844277	None:intergenic	45.0%
!	TGCTAAACCCTTTGTTTGGC+GGG	+	chr1.1:60844216-60844235	MS.gene52621:CDS	45.0%
!	TGGAGAGCTTTTCTTCCTGA+AGG	+	chr1.1:60844311-60844330	MS.gene52621:CDS	45.0%
!!	CATTGAAAGCATCGGACTCT+GGG	+	chr1.1:60844171-60844190	MS.gene52621:CDS	45.0%
!!	CTACATGGTTGGTGATAGCT+CGG	+	chr1.1:60844124-60844143	MS.gene52621:CDS	45.0%
!!	GAATCCGAAAAGCGTCTTGA+TGG	+	chr1.1:60844251-60844270	MS.gene52621:CDS	45.0%
!!	TACATGGTTGGTGATAGCTC+GGG	+	chr1.1:60844125-60844144	MS.gene52621:CDS	45.0%
!!	TGCTGGTTTGGATCTTGTTC+AGG	+	chr1.1:60844424-60844443	MS.gene52621:CDS	45.0%
	AATCTTCTCCGTCGTCCTGA+TGG	+	chr1.1:60843666-60843685	MS.gene52621:CDS	50.0%
	ATAGGCGTGCGCCTGAAAAT+AGG	+	chr1.1:60844009-60844028	MS.gene52621:CDS	50.0%
	CACCCATCTTCATAAGCGCA+AGG	-	chr1.1:60844038-60844057	None:intergenic	50.0%
	CAGCAGTAACAGGCTTCTCA+AGG	-	chr1.1:60843860-60843879	None:intergenic	50.0%
	CAGGACGGTAGATTCGAGTA+AGG	-	chr1.1:60843803-60843822	None:intergenic	50.0%
	CCAAAAGGTCGAAGCCTTGA+TGG	+	chr1.1:60844371-60844390	MS.gene52621:CDS	50.0%
	CCGTGGAAGAACATGACAAC+AGG	-	chr1.1:60843891-60843910	None:intergenic	50.0%
	CGAATCTACCGTCCTGTTGA+GGG	+	chr1.1:60843807-60843826	MS.gene52621:CDS	50.0%
	CTTGCGCTTATGAAGATGGG+TGG	+	chr1.1:60844036-60844055	MS.gene52621:CDS	50.0%
	CTTTCTTCCCCGCCAAACAA+AGG	-	chr1.1:60844227-60844246	None:intergenic	50.0%
	GCAAATGCGAATCCTGTGGA+TGG	+	chr1.1:60843735-60843754	MS.gene52621:CDS	50.0%
	GCAACCACCACAAGACTCTT+AGG	-	chr1.1:60844407-60844426	None:intergenic	50.0%
	GCCAAAGGACTTGAGAAAGC+TGG	+	chr1.1:60844464-60844483	MS.gene52621:CDS	50.0%
	GGAAGAACCTCAGCAGTAAC+AGG	-	chr1.1:60843870-60843889	None:intergenic	50.0%
	GTTCTTCTCCCTCAACAGGA+CGG	-	chr1.1:60843818-60843837	None:intergenic	50.0%
	GTTGTCATGTTCTTCCACGG+TGG	+	chr1.1:60843891-60843910	MS.gene52621:CDS	50.0%
	TAGGAAAGGCAACCCCATCA+AGG	-	chr1.1:60844388-60844407	None:intergenic	50.0%
	TCCACAGGATTCGCATTTGC+TGG	-	chr1.1:60843735-60843754	None:intergenic	50.0%
	TCCAGCAAATGCGAATCCTG+TGG	+	chr1.1:60843731-60843750	MS.gene52621:CDS	50.0%
	TCGAATCTACCGTCCTGTTG+AGG	+	chr1.1:60843806-60843825	MS.gene52621:CDS	50.0%
	TGAGAAGCCTGTTACTGCTG+AGG	+	chr1.1:60843860-60843879	MS.gene52621:CDS	50.0%
	TGATCTCTGTCTTCCCCTTC+AGG	-	chr1.1:60844329-60844348	None:intergenic	50.0%
!	AAGAGTCTTGTGGTGGTTGC+TGG	+	chr1.1:60844407-60844426	MS.gene52621:CDS	50.0%
!	CATCACGCGTGTAACCCTTT+TGG	+	chr1.1:60844347-60844366	MS.gene52621:CDS	50.0%
!	CCATCAAGGCTTCGACCTTT+TGG	-	chr1.1:60844374-60844393	None:intergenic	50.0%
!	GCTAAACCCTTTGTTTGGCG+GGG	+	chr1.1:60844217-60844236	MS.gene52621:CDS	50.0%
!	TCTTGTGGTGGTTGCTGGTT+TGG	+	chr1.1:60844412-60844431	MS.gene52621:CDS	50.0%
!!	GCATTGAAAGCATCGGACTC+TGG	+	chr1.1:60844170-60844189	MS.gene52621:CDS	50.0%
!!	GCTATTTCTCGAGCAAGCCA+CGG	+	chr1.1:60844502-60844521	MS.gene52621:CDS	50.0%
	TTCTCGAGCAAGCCACGGTT+GGG	+	chr1.1:60844507-60844526	MS.gene52621:CDS	55.0%
	TTTCTCGAGCAAGCCACGGT+TGG	+	chr1.1:60844506-60844525	MS.gene52621:CDS	55.0%
!!	AGCATCGGACTCTGGGATTC+CGG	+	chr1.1:60844178-60844197	MS.gene52621:CDS	55.0%
!!	ATGGTTGGTGATAGCTCGGG+CGG	+	chr1.1:60844128-60844147	MS.gene52621:CDS	55.0%
!!	GACTCTGGGATTCCGGTGTT+GGG	+	chr1.1:60844185-60844204	MS.gene52621:CDS	55.0%
!!	GGACTCTGGGATTCCGGTGT+TGG	+	chr1.1:60844184-60844203	MS.gene52621:CDS	60.0%
!!	TGGTTGGTGATAGCTCGGGC+GGG	+	chr1.1:60844129-60844148	MS.gene52621:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.1	gene	60843606	60844610	60843606	ID=MS.gene52621
chr1.1	mRNA	60843606	60844610	60843606	ID=MS.gene52621.t1;Parent=MS.gene52621
chr1.1	exon	60843606	60844610	60843606	ID=MS.gene52621.t1.exon1;Parent=MS.gene52621.t1
chr1.1	CDS	60843606	60844610	60843606	ID=cds.MS.gene52621.t1;Parent=MS.gene52621.t1

Gene Sequence

>MS.gene52621

ATGCAGATGGTGGTTCCATTGAACATGTGGGTTTTGATCTCCAATTTCAAGTTAGCATACAATCTTCTCCGTCGTCCTGATGGAACTTTCAACCGTGACTTGGCAGAGTTTCTTGATCGGAAAGTTCCAGCAAATGCGAATCCTGTGGATGGTGTTTTCTCTTTTGATGTTATTGTTGATCGGGAAACCAATCTCCTTACTCGAATCTACCGTCCTGTTGAGGGAGAAGAACAACATGTGAACATTGTTGACCTTGAGAAGCCTGTTACTGCTGAGGTTCTTCCTGTTGTCATGTTCTTCCACGGTGGAAGTTTTGCACATTCTTCGGCTAACAGTGCTATTTATGATACATTGTGTCGTCGATTGGTTGGTATTTGTAATGCTGTTGTCGTCTCGGTGAATTATAGGCGTGCGCCTGAAAATAGGTATCCTTGCGCTTATGAAGATGGGTGGAAAGCTGTTAAATGGGTTAACTCTAGAACTTGGCTGCAGAGTAAAAAAGATAGCAAGGTTCATATCTACATGGTTGGTGATAGCTCGGGCGGGAACATTGTGCACCATGTTGCATTGAAAGCATCGGACTCTGGGATTCCGGTGTTGGGAAATATACTGCTAAACCCTTTGTTTGGCGGGGAAGAAAGAACAGAATCCGAAAAGCGTCTTGATGGGAGATATTTTGTCAGAGTGAAAGATAGAGACTGGTATTGGAGAGCTTTTCTTCCTGAAGGGGAAGACAGAGATCATCACGCGTGTAACCCTTTTGGTCCAAAAGGTCGAAGCCTTGATGGGGTTGCCTTTCCTAAGAGTCTTGTGGTGGTTGCTGGTTTGGATCTTGTTCAGGATTGGCAATTGGGATATGCCAAAGGACTTGAGAAAGCTGGACAAAATGTGAAGTTGCTATTTCTCGAGCAAGCCACGGTTGGGTTTTACTTGCTACCAAATAATGAGCATTTCTCTGTTGTCATGGATGAGATAAAACATTTTGTCAACTCTGACTGTTCATAG

Protein sequence

>MS.gene52621.t1

MQMVVPLNMWVLISNFKLAYNLLRRPDGTFNRDLAEFLDRKVPANANPVDGVFSFDVIVDRETNLLTRIYRPVEGEEQHVNIVDLEKPVTAEVLPVVMFFHGGSFAHSSANSAIYDTLCRRLVGICNAVVVSVNYRRAPENRYPCAYEDGWKAVKWVNSRTWLQSKKDSKVHIYMVGDSSGGNIVHHVALKASDSGIPVLGNILLNPLFGGEERTESEKRLDGRYFVRVKDRDWYWRAFLPEGEDRDHHACNPFGPKGRSLDGVAFPKSLVVVAGLDLVQDWQLGYAKGLEKAGQNVKLLFLEQATVGFYLLPNNEHFSVVMDEIKHFVNSDCS