Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76851.t1 | RHN82242.1 | 97.8 | 405 | 6 | 1 | 1 | 402 | 231 | 635 | 1.10E-200 | 709.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76851.t1 | Q921I2 | 34.2 | 278 | 157 | 10 | 3 | 268 | 228 | 491 | 9.6e-31 | 136.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76851.t1 | A0A396JXG1 | 97.8 | 405 | 6 | 1 | 1 | 402 | 231 | 635 | 8.2e-201 | 709.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049435 | MS.gene76851 | 0.800924 | 1.15E-48 | -1.69E-46 |
| MS.gene050442 | MS.gene76851 | 0.802777 | 4.78E-49 | -1.69E-46 |
| MS.gene050844 | MS.gene76851 | 0.803236 | 3.84E-49 | -1.69E-46 |
| MS.gene054467 | MS.gene76851 | 0.803475 | 3.43E-49 | -1.69E-46 |
| MS.gene055356 | MS.gene76851 | 0.804456 | 2.14E-49 | -1.69E-46 |
| MS.gene055358 | MS.gene76851 | 0.823879 | 1.09E-53 | -1.69E-46 |
| MS.gene060167 | MS.gene76851 | 0.805297 | 1.43E-49 | -1.69E-46 |
| MS.gene060312 | MS.gene76851 | 0.835161 | 1.97E-56 | -1.69E-46 |
| MS.gene060322 | MS.gene76851 | 0.81232 | 4.50E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76851.t1 | MTR_1g107265 | 90.411 | 438 | 6 | 3 | 1 | 402 | 231 | 668 | 0.0 | 789 |
| MS.gene76851.t1 | MTR_1g107265 | 90.411 | 438 | 6 | 3 | 1 | 402 | 231 | 668 | 0.0 | 787 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76851.t1 | AT5G50310 | 65.610 | 410 | 127 | 7 | 1 | 402 | 231 | 634 | 2.25e-169 | 489 |
Find 96 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGTTCTTGAATGAGCTTTA+TGG | 0.213686 | 1.1:+76869655 | MS.gene76851:CDS |
| TAATCTTTGATGCAATATTC+TGG | 0.265820 | 1.1:-76870176 | None:intergenic |
| AAGAGGAGGGCTCTGCTATT+TGG | 0.302506 | 1.1:+76869252 | MS.gene76851:CDS |
| CAGTGTGCTGAGTGTGTTCA+TGG | 0.316496 | 1.1:-76873800 | None:intergenic |
| CCTGGAGCAATGTGGCCAAC+TGG | 0.354930 | 1.1:+76868680 | None:intergenic |
| AAGAGATGAAGCTGAGGATA+TGG | 0.355774 | 1.1:+76870122 | MS.gene76851:CDS |
| ATGCCTCCTGGTCCTCGTGC+AGG | 0.359177 | 1.1:+76869210 | MS.gene76851:CDS |
| GATTCCTTAGACTTTCCTTC+AGG | 0.366560 | 1.1:-76870228 | None:intergenic |
| CTTAGACTTTCCTTCAGGTT+TGG | 0.384258 | 1.1:-76870223 | None:intergenic |
| CTTGTTCCATTCCCAAGTCT+TGG | 0.391108 | 1.1:-76869027 | None:intergenic |
| CAAACCTGAAGGAAAGTCTA+AGG | 0.399665 | 1.1:+76870224 | MS.gene76851:CDS |
| TACAAATTTGTATTGGCAAA+TGG | 0.410540 | 1.1:+76873773 | MS.gene76851:CDS |
| TTGTTCCATTCCCAAGTCTT+GGG | 0.413147 | 1.1:-76869026 | None:intergenic |
| TCATCTCTTGCAGTAGATAG+TGG | 0.413970 | 1.1:-76870108 | None:intergenic |
| AGAGATACACTATATATATA+TGG | 0.419292 | 1.1:+76870423 | MS.gene76851:CDS |
| TTAAGCGTACAAATTTGTAT+TGG | 0.421780 | 1.1:+76873766 | MS.gene76851:CDS |
| ATTCCCAAGTCTTGGGATCA+AGG | 0.424658 | 1.1:-76869019 | None:intergenic |
| TTTCCATGTGTGTTCATAAG+AGG | 0.428365 | 1.1:+76869235 | MS.gene76851:CDS |
| GAAGGAAAGTCTAAGGAATC+TGG | 0.444652 | 1.1:+76870231 | MS.gene76851:CDS |
| AATCTTTGATGCAATATTCT+GGG | 0.445092 | 1.1:-76870175 | None:intergenic |
| ATCTTGCAGAAACACGATAC+AGG | 0.447457 | 1.1:+76875279 | MS.gene76851:CDS |
| TGCCTCCTGGTCCTCGTGCA+GGG | 0.455126 | 1.1:+76869211 | MS.gene76851:CDS |
| ATCTCTTCCAACAGCCATGC+AGG | 0.483354 | 1.1:-76870406 | None:intergenic |
| CCCAGGCATCAGAATCTGAA+TGG | 0.483703 | 1.1:+76871482 | MS.gene76851:intron |
| ACCTTAGCAAACTTGATGAA+TGG | 0.486761 | 1.1:+76870502 | MS.gene76851:CDS |
| GCAGGAATTAATGCGTCCAC+AGG | 0.496059 | 1.1:-76870388 | None:intergenic |
| CTGGAGCAATGTGGCCAACT+GGG | 0.504537 | 1.1:+76868681 | None:intergenic |
| TCCATTCATCAAGTTTGCTA+AGG | 0.506196 | 1.1:-76870503 | None:intergenic |
| GGTCCCTTGATCCCAAGACT+TGG | 0.508965 | 1.1:+76869015 | MS.gene76851:CDS |
| GACAAGAATGCCTCTGAGAA+AGG | 0.512410 | 1.1:+76868972 | MS.gene76851:CDS |
| TCTGCTATTTGGTGGTGTTG+TGG | 0.514556 | 1.1:+76869263 | MS.gene76851:CDS |
| AGGAGGGCTCTGCTATTTGG+TGG | 0.519472 | 1.1:+76869255 | MS.gene76851:CDS |
| TGAATGGAAGTGCATTATAC+CGG | 0.521369 | 1.1:+76870518 | MS.gene76851:CDS |
| AAGAAGAGTGGGATGCCTCC+TGG | 0.521741 | 1.1:+76869198 | MS.gene76851:CDS |
| AGATGCTGTAGCTTTAATCA+AGG | 0.526316 | 1.1:+76873413 | MS.gene76851:CDS |
| GAAAACCCTGCACGAGGACC+AGG | 0.526655 | 1.1:-76869216 | None:intergenic |
| CCATGTGTGTTCATAAGAGG+AGG | 0.533379 | 1.1:+76869238 | MS.gene76851:CDS |
| GGATAAATCAACAAAAGATA+AGG | 0.537288 | 1.1:+76869932 | MS.gene76851:CDS |
| TTCAGTTAGACACCAATCGC+TGG | 0.538855 | 1.1:+76869680 | MS.gene76851:CDS |
| ATTAATTCCTGCATGGCTGT+TGG | 0.539010 | 1.1:+76870399 | MS.gene76851:CDS |
| GAATTGTTCATTCAGATATG+TGG | 0.539563 | 1.1:+76868994 | MS.gene76851:CDS |
| GTACCCTCTGGAGTTAAGAA+AGG | 0.548785 | 1.1:+76869911 | MS.gene76851:CDS |
| CCAGGCATCAGAATCTGAAT+GGG | 0.549368 | 1.1:+76871483 | MS.gene76851:intron |
| CTTGGTAGGTTAAGAAGAGT+GGG | 0.551093 | 1.1:+76869187 | MS.gene76851:intron |
| TCTTGGTAGGTTAAGAAGAG+TGG | 0.551525 | 1.1:+76869186 | MS.gene76851:intron |
| TTTGTGAAATAGGTACCCTC+TGG | 0.553079 | 1.1:+76869899 | MS.gene76851:intron |
| TTCCCAAGTCTTGGGATCAA+GGG | 0.555002 | 1.1:-76869018 | None:intergenic |
| ATATATATATGGTGGCATGA+TGG | 0.561842 | 1.1:+76870434 | MS.gene76851:CDS |
| AAGTGTTTCATTGTCAGCAA+TGG | 0.562261 | 1.1:-76870199 | None:intergenic |
| TATCCTTTCTTAACTCCAGA+GGG | 0.568713 | 1.1:-76869914 | None:intergenic |
| AATCTAAACTTACCAGCGAT+TGG | 0.570438 | 1.1:-76869692 | None:intergenic |
| TGATAGGAACTCCGCAAGGA+TGG | 0.570747 | 1.1:+76875251 | MS.gene76851:intron |
| GTCCCTTGATCCCAAGACTT+GGG | 0.570914 | 1.1:+76869016 | MS.gene76851:CDS |
| CAAGACTTGGGAATGGAACA+AGG | 0.575951 | 1.1:+76869028 | MS.gene76851:CDS |
| AGTGTGCTGAGTGTGTTCAT+GGG | 0.577454 | 1.1:-76873799 | None:intergenic |
| TACTAACGATGTCGACAAGA+AGG | 0.579404 | 1.1:+76870080 | MS.gene76851:CDS |
| GAGCAAATCAGAGCTAGCCT+TGG | 0.579750 | 1.1:+76873480 | MS.gene76851:CDS |
| GGCATCAGAATCTGAATGGG+TGG | 0.581599 | 1.1:+76871486 | MS.gene76851:intron |
| CATTCAAAATTCTTTGCCCG+AGG | 0.582771 | 1.1:+76870266 | MS.gene76851:CDS |
| TTGATCCCAAGACTTGGGAA+TGG | 0.585173 | 1.1:+76869021 | MS.gene76851:CDS |
| TTATCCTTTCTTAACTCCAG+AGG | 0.586418 | 1.1:-76869915 | None:intergenic |
| TCTGAATGCATCAGATCAAG+TGG | 0.588802 | 1.1:+76873389 | MS.gene76851:CDS |
| CATGAACACACTCAGCACAC+TGG | 0.589985 | 1.1:+76873801 | MS.gene76851:CDS |
| TGTAGCTTTAATCAAGGGAG+GGG | 0.595643 | 1.1:+76873419 | MS.gene76851:CDS |
| CTGTAGCTTTAATCAAGGGA+GGG | 0.597224 | 1.1:+76873418 | MS.gene76851:CDS |
| TCTTGCAGAAACACGATACA+GGG | 0.600892 | 1.1:+76875280 | MS.gene76851:CDS |
| AGATTCACAGAGAACACCAT+TGG | 0.603261 | 1.1:+76873509 | MS.gene76851:CDS |
| GATGCTGTAGCTTTAATCAA+GGG | 0.604486 | 1.1:+76873414 | MS.gene76851:CDS |
| CTATTCAAAAGAAGTTTCAT+CGG | 0.608107 | 1.1:+76868950 | MS.gene76851:CDS |
| TGGACGCATTAATTCCTGCA+TGG | 0.609998 | 1.1:+76870392 | MS.gene76851:CDS |
| TGAAAACCACTACGCCCAGT+TGG | 0.612932 | 1.1:-76868695 | None:intergenic |
| ACGCTTAAAGAAATCTCTCA+AGG | 0.614690 | 1.1:-76873751 | None:intergenic |
| TCTGTGAATCTGACAAGCCA+AGG | 0.615736 | 1.1:-76873497 | None:intergenic |
| ATCTGCGTAGGAAAGAAAGA+AGG | 0.619000 | 1.1:+76873448 | MS.gene76851:CDS |
| CATGTGTGTTCATAAGAGGA+GGG | 0.619549 | 1.1:+76869239 | MS.gene76851:CDS |
| TGAATGGGTGGAAGTTTCAG+AGG | 0.621213 | 1.1:+76871498 | MS.gene76851:CDS |
| ATGTGGCCAACTGGGCGTAG+TGG | 0.621719 | 1.1:+76868689 | MS.gene76851:CDS |
| CCTCCTCTTATGAACACACA+TGG | 0.626591 | 1.1:-76869238 | None:intergenic |
| GTTGTGGATATAGAAGTTGA+AGG | 0.627219 | 1.1:+76869279 | MS.gene76851:CDS |
| TACTGCAAGAGATGAAGCTG+AGG | 0.628207 | 1.1:+76870116 | MS.gene76851:CDS |
| GAAACACTTACCAAACCTGA+AGG | 0.628495 | 1.1:+76870213 | MS.gene76851:CDS |
| GCTGTAGCTTTAATCAAGGG+AGG | 0.634285 | 1.1:+76873417 | MS.gene76851:CDS |
| CAGGAATTAATGCGTCCACA+GGG | 0.639638 | 1.1:-76870387 | None:intergenic |
| CACATGGAAAACCCTGCACG+AGG | 0.650088 | 1.1:-76869222 | None:intergenic |
| TAATAGTGATGGTACAAGTG+AGG | 0.660212 | 1.1:+76871564 | MS.gene76851:CDS |
| GATACACTATATATATATGG+TGG | 0.661154 | 1.1:+76870426 | MS.gene76851:CDS |
| GAGGGGTAAAGAATCTGCGT+AGG | 0.665128 | 1.1:+76873436 | MS.gene76851:CDS |
| CTGAATGCATCAGATCAAGT+GGG | 0.666302 | 1.1:+76873390 | MS.gene76851:CDS |
| GATGATGAATCTAATAGTGA+TGG | 0.667183 | 1.1:+76871553 | MS.gene76851:CDS |
| GTAGGAAAGAAAGAAGGTCA+CGG | 0.670544 | 1.1:+76873454 | MS.gene76851:CDS |
| AACCCTGCACGAGGACCAGG+AGG | 0.671017 | 1.1:-76869213 | None:intergenic |
| ATGAACAATTCCTTTCTCAG+AGG | 0.672557 | 1.1:-76868982 | None:intergenic |
| TTCTAGGCAGTGAAACCCTG+TGG | 0.688866 | 1.1:+76870372 | MS.gene76851:intron |
| TGGGTGATAGGAACTCCGCA+AGG | 0.694460 | 1.1:+76875247 | MS.gene76851:intron |
| GCTAAAACCTATTCTTGACG+AGG | 0.698872 | 1.1:+76875304 | MS.gene76851:CDS |
| CAAGATCAAAACCATCCTTG+CGG | 0.699328 | 1.1:-76875262 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 76868689 | 76875325 | 76868689 | ID=MS.gene76851 |
| chr1.1 | mRNA | 76868689 | 76875325 | 76868689 | ID=MS.gene76851.t1;Parent=MS.gene76851 |
| chr1.1 | exon | 76868689 | 76868739 | 76868689 | ID=MS.gene76851.t1.exon1;Parent=MS.gene76851.t1 |
| chr1.1 | CDS | 76868689 | 76868739 | 76868689 | ID=cds.MS.gene76851.t1;Parent=MS.gene76851.t1 |
| chr1.1 | exon | 76868933 | 76869049 | 76868933 | ID=MS.gene76851.t1.exon2;Parent=MS.gene76851.t1 |
| chr1.1 | CDS | 76868933 | 76869049 | 76868933 | ID=cds.MS.gene76851.t1;Parent=MS.gene76851.t1 |
| chr1.1 | exon | 76869195 | 76869300 | 76869195 | ID=MS.gene76851.t1.exon3;Parent=MS.gene76851.t1 |
| chr1.1 | CDS | 76869195 | 76869300 | 76869195 | ID=cds.MS.gene76851.t1;Parent=MS.gene76851.t1 |
| chr1.1 | exon | 76869638 | 76869701 | 76869638 | ID=MS.gene76851.t1.exon4;Parent=MS.gene76851.t1 |
| chr1.1 | CDS | 76869638 | 76869701 | 76869638 | ID=cds.MS.gene76851.t1;Parent=MS.gene76851.t1 |
| chr1.1 | exon | 76869911 | 76869953 | 76869911 | ID=MS.gene76851.t1.exon5;Parent=MS.gene76851.t1 |
| chr1.1 | CDS | 76869911 | 76869953 | 76869911 | ID=cds.MS.gene76851.t1;Parent=MS.gene76851.t1 |
| chr1.1 | exon | 76870057 | 76870287 | 76870057 | ID=MS.gene76851.t1.exon6;Parent=MS.gene76851.t1 |
| chr1.1 | CDS | 76870057 | 76870287 | 76870057 | ID=cds.MS.gene76851.t1;Parent=MS.gene76851.t1 |
| chr1.1 | exon | 76870378 | 76870539 | 76870378 | ID=MS.gene76851.t1.exon7;Parent=MS.gene76851.t1 |
| chr1.1 | CDS | 76870378 | 76870539 | 76870378 | ID=cds.MS.gene76851.t1;Parent=MS.gene76851.t1 |
| chr1.1 | exon | 76871487 | 76871618 | 76871487 | ID=MS.gene76851.t1.exon8;Parent=MS.gene76851.t1 |
| chr1.1 | CDS | 76871487 | 76871618 | 76871487 | ID=cds.MS.gene76851.t1;Parent=MS.gene76851.t1 |
| chr1.1 | exon | 76873387 | 76873530 | 76873387 | ID=MS.gene76851.t1.exon9;Parent=MS.gene76851.t1 |
| chr1.1 | CDS | 76873387 | 76873530 | 76873387 | ID=cds.MS.gene76851.t1;Parent=MS.gene76851.t1 |
| chr1.1 | exon | 76873741 | 76873827 | 76873741 | ID=MS.gene76851.t1.exon10;Parent=MS.gene76851.t1 |
| chr1.1 | CDS | 76873741 | 76873827 | 76873741 | ID=cds.MS.gene76851.t1;Parent=MS.gene76851.t1 |
| chr1.1 | exon | 76875257 | 76875325 | 76875257 | ID=MS.gene76851.t1.exon11;Parent=MS.gene76851.t1 |
| chr1.1 | CDS | 76875257 | 76875325 | 76875257 | ID=cds.MS.gene76851.t1;Parent=MS.gene76851.t1 |
| Gene Sequence |
| Protein sequence |
