Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76987.t1 | XP_013449816.1 | 91.8 | 49 | 4 | 0 | 1 | 49 | 76 | 124 | 1.00E-16 | 95.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76987.t1 | A0A072U2B1 | 91.8 | 49 | 4 | 0 | 1 | 49 | 76 | 124 | 7.2e-17 | 95.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049701 | MS.gene76987 | 0.826378 | 2.80E-54 | -1.69E-46 |
| MS.gene051710 | MS.gene76987 | 0.832803 | 7.67E-56 | -1.69E-46 |
| MS.gene055058 | MS.gene76987 | 0.833142 | 6.32E-56 | -1.69E-46 |
| MS.gene055091 | MS.gene76987 | 0.808401 | 3.15E-50 | -1.69E-46 |
| MS.gene058515 | MS.gene76987 | 0.860284 | 2.30E-63 | -1.69E-46 |
| MS.gene059584 | MS.gene76987 | 0.806716 | 7.19E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76987.t1 | MTR_7g095960 | 88.462 | 52 | 6 | 0 | 1 | 52 | 76 | 127 | 4.65e-28 | 102 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 11 sgRNAs with CRISPR-Local
Find 11 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGTGAAGCGTATGCAATTCT+TGG | 0.360237 | 6.1:-56864813 | MS.gene76987:CDS |
| GCGCTTGGTGGTGGTTCATC+TGG | 0.416876 | 6.1:-56864834 | MS.gene76987:CDS |
| GAACTTCACGCGCTTGGTGG+TGG | 0.467217 | 6.1:-56864843 | MS.gene76987:CDS |
| GACAGTGAACTTCACGCGCT+TGG | 0.484979 | 6.1:-56864849 | MS.gene76987:CDS |
| CTCAACGGAAACTTCGACGA+TGG | 0.526533 | 6.1:-56864876 | MS.gene76987:CDS |
| AGTGAACTTCACGCGCTTGG+TGG | 0.563216 | 6.1:-56864846 | MS.gene76987:CDS |
| AAGCGTATGCAATTCTTGGA+AGG | 0.577482 | 6.1:-56864809 | MS.gene76987:CDS |
| TGTGCTCGTAACTGCCTCAA+CGG | 0.603016 | 6.1:-56864891 | MS.gene76987:CDS |
| AAAGAATCATGAGAAGCACT+CGG | 0.639981 | 6.1:-56864919 | MS.gene76987:CDS |
| CATCGTCGAAGTTTCCGTTG+AGG | 0.692450 | 6.1:+56864877 | None:intergenic |
| AAGCGCGTGAAGTTCACTGT+CGG | 0.695670 | 6.1:+56864851 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAGAATCATGAGAAGCACT+CGG | - | chr6.1:56864823-56864842 | MS.gene76987:CDS | 35.0% |
| AAGCGTATGCAATTCTTGGA+AGG | - | chr6.1:56864933-56864952 | MS.gene76987:CDS | 40.0% | |
| GGTGAAGCGTATGCAATTCT+TGG | - | chr6.1:56864929-56864948 | MS.gene76987:CDS | 45.0% | |
| AAGCGCGTGAAGTTCACTGT+CGG | + | chr6.1:56864894-56864913 | None:intergenic | 50.0% | |
| CATCGTCGAAGTTTCCGTTG+AGG | + | chr6.1:56864868-56864887 | None:intergenic | 50.0% | |
| CTCAACGGAAACTTCGACGA+TGG | - | chr6.1:56864866-56864885 | MS.gene76987:CDS | 50.0% | |
| TGTGCTCGTAACTGCCTCAA+CGG | - | chr6.1:56864851-56864870 | MS.gene76987:CDS | 50.0% | |
| AGTGAACTTCACGCGCTTGG+TGG | - | chr6.1:56864896-56864915 | MS.gene76987:CDS | 55.0% | |
| GACAGTGAACTTCACGCGCT+TGG | - | chr6.1:56864893-56864912 | MS.gene76987:CDS | 55.0% | |
| ! | GAACTTCACGCGCTTGGTGG+TGG | - | chr6.1:56864899-56864918 | MS.gene76987:CDS | 60.0% |
| !! | GCGCTTGGTGGTGGTTCATC+TGG | - | chr6.1:56864908-56864927 | MS.gene76987:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.1 | gene | 56864803 | 56864961 | 56864803 | ID=MS.gene76987 |
| chr6.1 | mRNA | 56864803 | 56864961 | 56864803 | ID=MS.gene76987.t1;Parent=MS.gene76987 |
| chr6.1 | exon | 56864803 | 56864961 | 56864803 | ID=MS.gene76987.t1.exon1;Parent=MS.gene76987.t1 |
| chr6.1 | CDS | 56864803 | 56864961 | 56864803 | ID=cds.MS.gene76987.t1;Parent=MS.gene76987.t1 |
| Gene Sequence |
| Protein sequence |
