AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene77420

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene77420.t1 MTR_5g040360 96.880 609 19 0 1 609 1 609 0.0 1241
MS.gene77420.t1 MTR_8g055840 85.082 610 90 1 1 609 1 610 0.0 1109
MS.gene77420.t1 MTR_3g040370 72.896 594 157 3 15 608 17 606 0.0 943
MS.gene77420.t1 MTR_4g098890 65.802 617 194 6 2 609 3 611 0.0 879
MS.gene77420.t1 MTR_8g100075 53.055 622 261 7 9 608 17 629 0.0 697
MS.gene77420.t1 MTR_1g101370 55.853 598 245 8 18 600 25 618 0.0 696
MS.gene77420.t1 MTR_0019s0120 54.918 610 251 8 18 608 31 635 0.0 692
MS.gene77420.t1 MTR_7g076170 53.440 625 260 8 4 601 7 627 0.0 692
MS.gene77420.t1 MTR_8g432680 51.803 610 265 8 17 601 27 632 0.0 665
MS.gene77420.t1 MTR_7g006060 50.755 530 248 4 77 593 36 565 0.0 567
MS.gene77420.t1 MTR_0019s0120 53.242 509 214 8 18 507 31 534 0.0 546
MS.gene77420.t1 MTR_8g071280 46.095 525 272 7 84 601 70 590 1.26e-164 483
MS.gene77420.t1 MTR_5g026930 42.010 607 321 10 2 599 6 590 1.84e-162 478
MS.gene77420.t1 MTR_7g076150 43.085 564 292 8 49 599 40 587 9.59e-162 475
MS.gene77420.t1 MTR_8g432620 42.569 545 284 8 69 600 73 601 1.61e-160 473
MS.gene77420.t1 MTR_5g080720 43.364 535 277 11 75 596 116 637 4.86e-152 452
MS.gene77420.t1 MTR_1g442840 40.394 609 308 14 1 575 1 588 6.89e-147 439
MS.gene77420.t1 MTR_1g069785 39.680 625 315 17 1 601 1 587 2.66e-146 436
MS.gene77420.t1 MTR_1g075900 42.397 559 287 12 73 609 79 624 5.75e-144 431
MS.gene77420.t1 MTR_3g068990 43.715 533 266 11 82 597 151 666 5.72e-143 430
MS.gene77420.t1 MTR_1g442840 45.303 479 235 9 111 575 10 475 3.82e-142 422
MS.gene77420.t1 MTR_1g029730 43.396 530 273 13 84 598 141 658 9.74e-141 424
MS.gene77420.t1 MTR_7g076150 44.662 459 225 8 154 599 2 444 1.94e-137 408
MS.gene77420.t1 MTR_5g080720 43.579 475 242 11 75 536 116 577 4.11e-133 402
MS.gene77420.t1 MTR_5g026930 41.553 515 271 9 2 507 6 499 9.08e-133 398
MS.gene77420.t1 MTR_5g083150 38.462 559 294 16 58 599 73 598 6.96e-131 397
MS.gene77420.t1 MTR_3g096480 41.445 526 272 14 93 599 268 776 1.19e-126 392
MS.gene77420.t1 MTR_5g021100 41.120 518 269 15 93 590 329 830 2.92e-126 392
MS.gene77420.t1 MTR_8g076650 41.811 519 263 15 93 590 387 887 4.32e-125 390
MS.gene77420.t1 MTR_3g071530 38.475 551 293 17 62 599 129 646 1.26e-124 382
MS.gene77420.t1 MTR_1g051055 39.139 534 285 13 87 599 89 603 3.18e-124 380
MS.gene77420.t1 MTR_8g093880 40.607 527 275 15 93 599 298 806 5.99e-123 383
MS.gene77420.t1 MTR_3g088790 40.076 529 276 16 93 599 233 742 5.09e-120 373
MS.gene77420.t1 MTR_4g083030 31.579 551 336 12 70 598 72 603 7.61e-92 295
MS.gene77420.t1 MTR_3g074780 30.769 572 341 17 70 604 140 693 1.70e-88 289
MS.gene77420.t1 MTR_1g022255 32.959 534 307 15 84 599 199 699 7.44e-87 285
MS.gene77420.t1 MTR_5g064080 30.035 566 333 16 70 599 132 670 3.85e-84 277
MS.gene77420.t1 MTR_1g022255 28.500 400 241 12 84 466 199 570 1.15e-42 162
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene77420.t1 AT1G26850 77.724 615 127 3 3 609 4 616 0.0 1023
MS.gene77420.t1 AT1G26850 77.724 615 127 3 3 609 4 616 0.0 1023
MS.gene77420.t1 AT4G18030 66.333 600 190 4 9 601 11 605 0.0 857
MS.gene77420.t1 AT1G26850 76.200 500 111 2 3 494 4 503 0.0 818
MS.gene77420.t1 AT4G10440 54.708 616 245 6 9 601 14 618 0.0 710
MS.gene77420.t1 AT1G33170 51.548 646 264 7 1 604 1 639 0.0 692
MS.gene77420.t1 AT4G00750 51.307 612 283 5 5 601 13 624 0.0 666
MS.gene77420.t1 AT2G45750 51.356 627 276 9 5 607 7 628 0.0 659
MS.gene77420.t1 AT2G43200 52.023 519 238 5 84 594 91 606 0.0 559
MS.gene77420.t1 AT2G43200 52.023 519 238 5 84 594 91 606 0.0 559
MS.gene77420.t1 AT1G31850 45.506 534 275 9 84 607 76 603 1.88e-168 493
MS.gene77420.t1 AT1G31850 45.506 534 275 9 84 607 76 603 1.88e-168 493
MS.gene77420.t1 AT1G31850 45.506 534 275 9 84 607 76 603 1.88e-168 493
MS.gene77420.t1 AT1G31850 45.506 534 275 9 84 607 76 603 1.88e-168 493
MS.gene77420.t1 AT4G19120 43.478 552 296 9 70 609 53 600 4.12e-166 487
MS.gene77420.t1 AT4G19120 43.478 552 296 9 70 609 53 600 4.12e-166 487
MS.gene77420.t1 AT4G00740 45.865 532 259 9 87 605 84 599 3.99e-164 482
MS.gene77420.t1 AT4G00740 45.865 532 259 9 87 605 84 599 3.99e-164 482
MS.gene77420.t1 AT5G06050 42.992 528 275 11 84 598 149 663 2.10e-145 437
MS.gene77420.t1 AT1G04430 40.577 589 314 12 8 576 9 581 2.79e-143 429
MS.gene77420.t1 AT1G04430 40.577 589 314 12 8 576 9 581 2.79e-143 429
MS.gene77420.t1 AT1G04430 40.577 589 314 12 8 576 9 581 2.79e-143 429
MS.gene77420.t1 AT2G39750 41.316 547 295 11 66 597 155 690 1.91e-142 429
MS.gene77420.t1 AT3G23300 42.620 542 276 13 81 601 76 603 2.07e-141 424
MS.gene77420.t1 AT3G23300 42.620 542 276 13 81 601 76 603 2.07e-141 424
MS.gene77420.t1 AT4G14360 43.860 513 257 11 81 575 73 572 3.13e-141 424
MS.gene77420.t1 AT4G14360 43.860 513 257 11 81 575 73 572 3.13e-141 424
MS.gene77420.t1 AT1G77260 41.869 535 283 12 76 597 135 654 7.40e-138 416
MS.gene77420.t1 AT4G19120 44.444 450 235 8 70 507 53 499 7.87e-136 406
MS.gene77420.t1 AT2G34300 40.339 590 312 17 38 606 194 764 2.94e-134 411
MS.gene77420.t1 AT2G34300 40.339 590 312 17 38 606 194 764 2.94e-134 411
MS.gene77420.t1 AT2G34300 40.339 590 312 17 38 606 194 764 2.94e-134 411
MS.gene77420.t1 AT1G29470 41.760 534 277 12 93 609 250 766 8.49e-134 409
MS.gene77420.t1 AT1G29470 41.760 534 277 12 93 609 250 766 8.49e-134 409
MS.gene77420.t1 AT2G40280 40.341 528 271 13 87 599 83 581 3.55e-133 402
MS.gene77420.t1 AT5G14430 39.384 617 317 19 11 599 17 604 6.86e-132 400
MS.gene77420.t1 AT5G04060 39.205 528 290 9 87 599 86 597 8.21e-131 396
MS.gene77420.t1 AT5G14430 39.060 617 319 19 11 599 17 604 3.12e-130 395
MS.gene77420.t1 AT3G56080 39.923 521 273 12 93 599 114 608 6.03e-128 389
MS.gene77420.t1 AT3G10200 38.649 533 288 12 87 599 75 588 1.30e-126 385
MS.gene77420.t1 AT5G64030 41.782 505 264 12 93 579 308 800 3.37e-126 392
MS.gene77420.t1 AT3G51070 41.418 536 270 19 92 604 380 894 1.02e-125 392
MS.gene77420.t1 AT3G10200 40.166 483 251 11 87 550 75 538 2.53e-118 364
MS.gene77420.t1 AT3G56080 41.333 450 232 11 93 528 114 545 4.25e-113 349
MS.gene77420.t1 AT3G51070 42.795 458 226 17 92 528 380 822 1.19e-107 343
MS.gene77420.t1 AT1G13860 32.332 566 322 15 58 584 45 588 2.73e-93 299
MS.gene77420.t1 AT1G13860 32.332 566 322 15 58 584 45 588 2.73e-93 299
MS.gene77420.t1 AT1G13860 32.332 566 322 15 58 584 45 588 2.73e-93 299
MS.gene77420.t1 AT1G13860 32.332 566 322 15 58 584 45 588 2.73e-93 299
MS.gene77420.t1 AT2G03480 31.230 634 350 17 3 598 7 592 3.45e-91 293
MS.gene77420.t1 AT2G03480 31.051 628 370 15 3 598 7 603 2.32e-90 291
MS.gene77420.t1 AT1G19430 33.142 522 304 14 93 599 231 722 7.58e-87 285
MS.gene77420.t1 AT1G19430 33.142 522 304 14 93 599 231 722 7.58e-87 285
MS.gene77420.t1 AT1G13860 35.227 440 248 11 171 584 4 432 3.80e-85 273
MS.gene77420.t1 AT1G78240 31.206 564 331 17 74 598 133 678 2.52e-84 278
MS.gene77420.t1 AT1G78240 31.206 564 331 17 74 598 133 678 2.52e-84 278
MS.gene77420.t1 AT2G03480 30.488 410 247 9 3 393 7 397 7.74e-50 179
MS.gene77420.t1 AT1G19430 29.231 390 237 11 93 468 231 595 7.49e-45 169

Find 167 sgRNAs with CRISPR-Local

Find 226 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGCGGTTATTGGGTGCTTTC+GGG 0.168627 5.1:-30466017 MS.gene77420:CDS
TGAGAATCAAAGCTTAAATT+TGG 0.224891 5.1:+30467137 None:intergenic
CCAGTTGGGGTGCATATCTT+TGG 0.240419 5.1:-30466349 MS.gene77420:CDS
TTCTAAGACCTGGCGGTTAT+TGG 0.250522 5.1:-30466028 MS.gene77420:CDS
TGGCGGTTATTGGGTGCTTT+CGG 0.251157 5.1:-30466018 MS.gene77420:CDS
TTCTCGTTGCTTAATTCCTT+GGG 0.256373 5.1:-30466174 MS.gene77420:CDS
TGTTGTTTCTTCTACATATT+AGG 0.267388 5.1:-30467242 MS.gene77420:CDS
TGTCAGCCCCTTGAGGGAAT+TGG 0.275822 5.1:+30466766 None:intergenic
GTTCTCGTTGCTTAATTCCT+TGG 0.299920 5.1:-30466175 MS.gene77420:CDS
TCGTTGCAGGTATAAGAAAA+TGG 0.307086 5.1:-30465598 MS.gene77420:intron
TCCATCTGGAGCCTTTGATA+TGG 0.309219 5.1:-30466204 MS.gene77420:CDS
TGAACACATTTCCCTCATAT+TGG 0.312865 5.1:+30466808 None:intergenic
GACAGCAATTTCATTCTTCT+CGG 0.325154 5.1:+30465878 None:intergenic
TAACATAGATGATATTCTTC+TGG 0.332182 5.1:-30464533 MS.gene77420:CDS
GTTGCCAGTGGTTCTGTTCC+CGG 0.338306 5.1:-30465495 MS.gene77420:CDS
ATGGCTAAATCAATTTCTTC+TGG 0.338989 5.1:-30467311 MS.gene77420:CDS
GTGCAGATCTTTATAGTAGT+TGG 0.347054 5.1:-30467269 MS.gene77420:CDS
GTCAGCCCCTTGAGGGAATT+GGG 0.348985 5.1:+30466767 None:intergenic
CGGCTCTTTGGCCATGGAAA+TGG 0.358252 5.1:+30466900 None:intergenic
CTATTCAATCTCCTAAGTTA+TGG 0.359289 5.1:-30465329 MS.gene77420:CDS
TCATAGGCATTTATCATGAT+TGG 0.359736 5.1:-30465242 MS.gene77420:intron
GGAACATAATCACGGCTCTT+TGG 0.368346 5.1:+30466888 None:intergenic
ATAAAATTGCCTTATCCATC+TGG 0.370154 5.1:-30466218 MS.gene77420:CDS
CGAAGTAGACAAGAAGATTC+TGG 0.373051 5.1:-30465825 MS.gene77420:CDS
AATTGCTGTCAAGCAGTATT+GGG 0.374007 5.1:-30464359 MS.gene77420:CDS
CGGAATATCACAGCACCTTC+AGG 0.375594 5.1:+30464490 None:intergenic
CATGGATATGAATGCTGGTT+TGG 0.377343 5.1:-30465367 MS.gene77420:CDS
ACAGAACCACTGGCAACTTT+AGG 0.382460 5.1:+30465501 None:intergenic
GCGTGGCAGAGAACTGGTTT+TGG 0.384451 5.1:-30467218 MS.gene77420:CDS
AGGTTGCTAAACTCCTTTGC+TGG 0.388550 5.1:-30465911 MS.gene77420:CDS
TCTAAGACCTGGCGGTTATT+GGG 0.391716 5.1:-30466027 MS.gene77420:CDS
TTGTAGGGTGCGTTCGCATA+TGG 0.395645 5.1:+30466867 None:intergenic
CGCTATGTCATTTGCACCTA+GGG 0.403385 5.1:-30466312 MS.gene77420:CDS
TATTCAATCTCCTAAGTTAT+GGG 0.408342 5.1:-30465328 MS.gene77420:CDS
ATAAATAAGCTCCTGGACTC+TGG 0.414706 5.1:-30465405 MS.gene77420:CDS
TCTTCCTCAAGATCCTCCTT+AGG 0.414914 5.1:+30465951 None:intergenic
CTCAACTGTAAGACTCTTGT+AGG 0.415831 5.1:+30466851 None:intergenic
ATCAAGGTAAAGAAAATTGT+TGG 0.416395 5.1:-30464448 MS.gene77420:CDS
TAATTAGCCTTCCAATGGAT+TGG 0.416920 5.1:+30465990 None:intergenic
TAATTGCTGTCAAGCAGTAT+TGG 0.427048 5.1:-30464360 MS.gene77420:CDS
AGTACAGTTTGCACTTGAAA+GGG 0.428802 5.1:-30466273 MS.gene77420:CDS
ATCCTCCTTAGGTCTTTGCC+AGG 0.433073 5.1:+30465962 None:intergenic
TCAGAACTGGATCCAATATG+AGG 0.434365 5.1:-30466820 MS.gene77420:CDS
CAATTGGAGGTTACTAAGAA+AGG 0.434885 5.1:-30467179 MS.gene77420:CDS
ATTTATCAGCGAGGACTCAT+AGG 0.437788 5.1:-30465258 MS.gene77420:CDS
CCAAAGATATGCACCCCAAC+TGG 0.442920 5.1:+30466349 None:intergenic
GCATATAGTCTCTGTGGAAA+AGG 0.443799 5.1:+30465531 None:intergenic
CACCTTCAGGTCGTAAAATC+CGG 0.448926 5.1:+30464503 None:intergenic
TTGCAAGGCTTAAATACCTT+AGG 0.449525 5.1:+30467071 None:intergenic
GGCACGCCGTTGATCATGGC+AGG 0.451114 5.1:+30467025 None:intergenic
CAACAGATGCTGATGATGTT+TGG 0.451762 5.1:-30465785 MS.gene77420:intron
GGCAGATGGGATTTATATGA+TGG 0.451926 5.1:-30466063 MS.gene77420:intron
GTATGTCTCAGCTGAAACTC+CGG 0.453655 5.1:+30465476 None:intergenic
GAGGTTACTAAGAAAGGTGC+TGG 0.454381 5.1:-30467173 MS.gene77420:CDS
AAGTACAGTTTGCACTTGAA+AGG 0.454812 5.1:-30466274 MS.gene77420:CDS
TTAATTCCTTGGGGTTCAAA+TGG 0.459240 5.1:-30466164 MS.gene77420:intron
TCTCCGTGGACTTTACGATT+AGG 0.459427 5.1:+30465561 None:intergenic
TTAGGTGCGTGGCAGAGAAC+TGG 0.459773 5.1:-30467224 MS.gene77420:CDS
CCTGCCGTCATTGGTGTCCT+AGG 0.462243 5.1:-30466245 MS.gene77420:CDS
ACAGCACTGGACACTGGTTG+TGG 0.462399 5.1:-30466690 MS.gene77420:CDS
TATGAGATGGGATACTAAAC+TGG 0.463047 5.1:-30464422 MS.gene77420:CDS
ATGGATATGAATGCTGGTTT+GGG 0.470981 5.1:-30465366 MS.gene77420:CDS
TTGAGAAGGCTATTCAGAAC+TGG 0.479914 5.1:-30466833 MS.gene77420:CDS
TATATTTGTCAGCCCCTTGA+GGG 0.481593 5.1:+30466760 None:intergenic
GGGAATTGGGTTCCACCACC+AGG 0.486235 5.1:+30466780 None:intergenic
CAAGAAAATAAATAAGCTCC+TGG 0.495511 5.1:-30465412 MS.gene77420:CDS
TGAATGAGGTCGTACGTCCT+TGG 0.496095 5.1:+30464805 None:intergenic
CTGACTGTTCCATTCTCTAT+CGG 0.496657 5.1:+30466714 None:intergenic
GTAACATACCCTTTCGGTGC+TGG 0.499813 5.1:+30466924 None:intergenic
GTATGTTTCTCAGCTATAGT+AGG 0.501052 5.1:+30465291 None:intergenic
ATATTCTTCTGGAGATGGAC+CGG 0.502226 5.1:-30464522 MS.gene77420:CDS
TAATCACGGCTCTTTGGCCA+TGG 0.503657 5.1:+30466894 None:intergenic
TGGAAGGTATCGCAATATCA+TGG 0.504476 5.1:-30465385 MS.gene77420:CDS
TCATGGCACGCCGTTGATCA+TGG 0.504850 5.1:+30467021 None:intergenic
ACGACCTCATTCATAGCAAT+GGG 0.505409 5.1:-30464795 MS.gene77420:CDS
TGCGATACCTTCCAGAGTCC+AGG 0.507113 5.1:+30465394 None:intergenic
GGAAGGCTAATTACAAAGCC+TGG 0.510311 5.1:-30465980 MS.gene77420:CDS
GTCGTACGTCCTTGGATATG+TGG 0.511535 5.1:+30464813 None:intergenic
TCAAAGGCTCCAGATGGATA+AGG 0.515562 5.1:+30466209 None:intergenic
CTTTGTAATTAGCCTTCCAA+TGG 0.517155 5.1:+30465985 None:intergenic
ACCAGGATGATAACGAAAAG+TGG 0.518679 5.1:-30465455 MS.gene77420:CDS
TACGACCTCATTCATAGCAA+TGG 0.523430 5.1:-30464796 MS.gene77420:CDS
TGAGGTTGATTCAAAACCTA+AGG 0.526700 5.1:-30467087 MS.gene77420:CDS
AATGGAGTAACATACCCTTT+CGG 0.527057 5.1:+30466918 None:intergenic
AATATCATGGATATGAATGC+TGG 0.527971 5.1:-30465372 MS.gene77420:CDS
CAGCACTGGACACTGGTTGT+GGG 0.532258 5.1:-30466689 MS.gene77420:CDS
CGGTCTTCTTCTCCCAGCAA+AGG 0.532439 5.1:+30465898 None:intergenic
TTCTCACCATTTGAACCCCA+AGG 0.535398 5.1:+30466158 None:intergenic
AGAAGAATGAAATTGCTGTC+TGG 0.538296 5.1:-30465875 MS.gene77420:CDS
GTTTGATACCAGCACCGAAA+GGG 0.539221 5.1:-30466932 MS.gene77420:CDS
AGGGGTGTACCTGCCGTCAT+TGG 0.542782 5.1:-30466254 MS.gene77420:CDS
CAAAGCCTGGCAAAGACCTA+AGG 0.543305 5.1:-30465967 MS.gene77420:CDS
AGATGATATTCTTCTGGAGA+TGG 0.544792 5.1:-30464527 MS.gene77420:CDS
GGTTGCTAAACTCCTTTGCT+GGG 0.545805 5.1:-30465910 MS.gene77420:CDS
CAAGAGTCTTACAGTTGAGA+AGG 0.546705 5.1:-30466847 MS.gene77420:CDS
TTTGCACAGGTTGCCAGTTG+GGG 0.547748 5.1:-30466362 MS.gene77420:intron
TGAAGTTGATGTCTTAATCA+AGG 0.548028 5.1:-30464464 MS.gene77420:CDS
TCTGTGATACCGATAGAGAA+TGG 0.548272 5.1:-30466723 MS.gene77420:CDS
CGATAGAGAATGGAACAGTC+AGG 0.551422 5.1:-30466713 MS.gene77420:CDS
TTAGCCTTCCAATGGATTGG+AGG 0.556029 5.1:+30465993 None:intergenic
AAATTGTTGGAGGTATGAGA+TGG 0.556242 5.1:-30464435 MS.gene77420:CDS
GAACAAGTGGACCGTCCTCA+TGG 0.559718 5.1:+30464395 None:intergenic
TTTCGGGACCTCCAATCCAT+TGG 0.560894 5.1:-30466001 MS.gene77420:CDS
ACATTCATGACCCATAACTT+AGG 0.560995 5.1:+30465318 None:intergenic
TCGCTATGTCATTTGCACCT+AGG 0.561599 5.1:-30466313 MS.gene77420:CDS
TGTGCTTCATGTGAGTCCCT+AGG 0.562927 5.1:+30466296 None:intergenic
AATTGTTGGAGGTATGAGAT+GGG 0.562974 5.1:-30464434 MS.gene77420:CDS
GAACCACTGGCAACTTTAGG+AGG 0.564388 5.1:+30465504 None:intergenic
TGGAACAGTCAGGACAGCAC+TGG 0.564499 5.1:-30466703 MS.gene77420:CDS
AGAACAAAGAAAGATTGAAG+AGG 0.565209 5.1:-30465931 MS.gene77420:CDS
GGAAATGTGTTCAGATTCCC+TGG 0.566798 5.1:-30466798 MS.gene77420:CDS
GTGTTCAGATTCCCTGGTGG+TGG 0.569353 5.1:-30466792 MS.gene77420:CDS
CAGAACTGGATCCAATATGA+GGG 0.569553 5.1:-30466819 MS.gene77420:CDS
TCGATATATCGAGCCTTGCA+AGG 0.569809 5.1:+30467056 None:intergenic
AACTCCGGGAACAGAACCAC+TGG 0.570803 5.1:+30465491 None:intergenic
GTCAGGACAGCACTGGACAC+TGG 0.572331 5.1:-30466696 MS.gene77420:CDS
AAGGTAAAGAAAATTGTTGG+AGG 0.572644 5.1:-30464445 MS.gene77420:CDS
GGGACCTCCAATCCATTGGA+AGG 0.573733 5.1:-30465997 MS.gene77420:CDS
TAAGGTATTTAAGCCTTGCA+AGG 0.576659 5.1:-30467069 MS.gene77420:CDS
TATGTCTCAGCTGAAACTCC+GGG 0.580149 5.1:+30465477 None:intergenic
ATAAGCTCCTGGACTCTGGA+AGG 0.581133 5.1:-30465401 MS.gene77420:CDS
AGAACAATGTGCCATATCAA+AGG 0.583862 5.1:+30466193 None:intergenic
GTGGAACCCAATTCCCTCAA+GGG 0.584099 5.1:-30466773 MS.gene77420:CDS
AACCGCCAGGTCTTAGAACT+CGG 0.584438 5.1:+30466033 None:intergenic
ACACGCCCTGCCATGATCAA+CGG 0.586626 5.1:-30467031 MS.gene77420:CDS
GAAAGCACCCAATAACCGCC+AGG 0.587918 5.1:+30466020 None:intergenic
AAAATATGATTTATCGAGAG+AGG 0.590909 5.1:-30466986 MS.gene77420:CDS
GCCATATCAAAGGCTCCAGA+TGG 0.590985 5.1:+30466203 None:intergenic
TTAAGCTTTGATTCTCACCA+TGG 0.593567 5.1:-30467131 MS.gene77420:CDS
AAGACCTAAGGAGGATCTTG+AGG 0.593947 5.1:-30465955 MS.gene77420:CDS
AGCACTGGACACTGGTTGTG+GGG 0.594228 5.1:-30466688 MS.gene77420:intron
GGTGGAACCCAATTCCCTCA+AGG 0.594921 5.1:-30466774 MS.gene77420:CDS
ATATATTTGTCAGCCCCTTG+AGG 0.597173 5.1:+30466759 None:intergenic
AGAAGTTAGCAAAATTGATG+AGG 0.600153 5.1:-30467105 MS.gene77420:CDS
GGTATGTTACTCCATTTCCA+TGG 0.603095 5.1:-30466911 MS.gene77420:CDS
TCAACTGTAAGACTCTTGTA+GGG 0.608324 5.1:+30466852 None:intergenic
TGTTTGATACCAGCACCGAA+AGG 0.609194 5.1:-30466933 MS.gene77420:CDS
TCTCGTTGCTTAATTCCTTG+GGG 0.611394 5.1:-30466173 MS.gene77420:CDS
AGTTTCAGCTGAGACATACC+AGG 0.611606 5.1:-30465472 MS.gene77420:CDS
GTTCCTCCTAAAGTTGCCAG+TGG 0.612508 5.1:-30465507 MS.gene77420:CDS
CCTAGGACACCAATGACGGC+AGG 0.614190 5.1:+30466245 None:intergenic
TACTAAACTGGTTGACCATG+AGG 0.614521 5.1:-30464410 MS.gene77420:CDS
TTTACGATTAGGAGTAACAC+AGG 0.615416 5.1:+30465572 None:intergenic
TGGGCTCTTTAGTCTGTACA+AGG 0.617149 5.1:-30464776 MS.gene77420:CDS
AGAGCCCATTGCTATGAATG+AGG 0.631489 5.1:+30464791 None:intergenic
GCACGCCGTTGATCATGGCA+GGG 0.632559 5.1:+30467026 None:intergenic
GGTTCCTAGGACACCAATGA+CGG 0.632608 5.1:+30466241 None:intergenic
AGCCTGGCAAAGACCTAAGG+AGG 0.634638 5.1:-30465964 MS.gene77420:CDS
GTTGACCGAGTTCTAAGACC+TGG 0.636678 5.1:-30466038 MS.gene77420:CDS
GTACAGTTTGCACTTGAAAG+GGG 0.638641 5.1:-30466272 MS.gene77420:CDS
TCTTCTACATATTAGGTGCG+TGG 0.640208 5.1:-30467235 MS.gene77420:CDS
TGGAACCCAATTCCCTCAAG+GGG 0.646344 5.1:-30466772 MS.gene77420:CDS
TGTAACTTCTCTTCTTCACG+AGG 0.648313 5.1:+30466957 None:intergenic
GGAACTGCATATAGTCTCTG+TGG 0.648475 5.1:+30465525 None:intergenic
ACTCCTAATCGTAAAGTCCA+CGG 0.651226 5.1:-30465564 MS.gene77420:CDS
AAACTGGTTGACCATGAGGA+CGG 0.653450 5.1:-30464406 MS.gene77420:CDS
TCGCATATGGAACATAATCA+CGG 0.658822 5.1:+30466880 None:intergenic
AGGTTACTAAGAAAGGTGCT+GGG 0.674354 5.1:-30467172 MS.gene77420:CDS
GGAATTGGGTTCCACCACCA+GGG 0.677362 5.1:+30466781 None:intergenic
GACCGAGTTCTAAGACCTGG+CGG 0.681581 5.1:-30466035 MS.gene77420:CDS
TTAAGACATCAACTTCATCA+CGG 0.685496 5.1:+30464470 None:intergenic
CTCGGTGTCATTTATCAGCG+AGG 0.696680 5.1:-30465267 MS.gene77420:CDS
GTAACTTCTCTTCTTCACGA+GGG 0.701314 5.1:+30466958 None:intergenic
GAGTGGTTATTGTGTTGACG+TGG 0.705177 5.1:+30464321 None:intergenic
GAAAAGGCTTAAGATCTCCG+TGG 0.706045 5.1:+30465547 None:intergenic
ATAGCTGAGAAACATACACT+CGG 0.706522 5.1:-30465285 MS.gene77420:CDS
AATGTGTTCAGATTCCCTGG+TGG 0.757911 5.1:-30466795 MS.gene77420:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGGCATAAATAAATAAATA+AGG + chr5.1:30466781-30466800 None:intergenic 15.0%
!! TTGAATAACCATATAATTAA+AGG + chr5.1:30466901-30466920 None:intergenic 15.0%
!! TGAGTATTCCTTTAATTATA+TGG - chr5.1:30466890-30466909 MS.gene77420:CDS 20.0%
!!! AATCATATTTTGTCTAGAAA+AGG + chr5.1:30464643-30464662 None:intergenic 20.0%
! AAAATATGATTTATCGAGAG+AGG - chr5.1:30464652-30464671 MS.gene77420:intron 25.0%
! AATGAATGGAATATCAACAA+TGG + chr5.1:30465922-30465941 None:intergenic 25.0%
! ATCAAGGTAAAGAAAATTGT+TGG - chr5.1:30467190-30467209 MS.gene77420:CDS 25.0%
! GAATGCATTATAAGTTGTAT+AGG - chr5.1:30466950-30466969 MS.gene77420:CDS 25.0%
! GAGATAGAGATAAACATATA+CGG - chr5.1:30466617-30466636 MS.gene77420:intron 25.0%
! TAACATAGATGATATTCTTC+TGG - chr5.1:30467105-30467124 MS.gene77420:CDS 25.0%
! TATTCAATCTCCTAAGTTAT+GGG - chr5.1:30466310-30466329 MS.gene77420:CDS 25.0%
! TGAGAATCAAAGCTTAAATT+TGG + chr5.1:30464504-30464523 None:intergenic 25.0%
! TGTTAGTAGAACTAAATGAA+TGG + chr5.1:30465936-30465955 None:intergenic 25.0%
! TTATAATGCATTCTATGCAA+AGG + chr5.1:30466943-30466962 None:intergenic 25.0%
!! ATTAGCAGTATATTTAGTTC+AGG - chr5.1:30465043-30465062 MS.gene77420:intron 25.0%
!! TTAGCAGTATATTTAGTTCA+GGG - chr5.1:30465044-30465063 MS.gene77420:intron 25.0%
!!! TGTTGTTTCTTCTACATATT+AGG - chr5.1:30464396-30464415 MS.gene77420:CDS 25.0%
AAGAGAGAACTAAAACTAAG+TGG + chr5.1:30467019-30467038 None:intergenic 30.0%
AAGGAAAAACATGTGAGAAA+AGG + chr5.1:30466762-30466781 None:intergenic 30.0%
AAGGTAAAGAAAATTGTTGG+AGG - chr5.1:30467193-30467212 MS.gene77420:CDS 30.0%
AATATCATGGATATGAATGC+TGG - chr5.1:30466266-30466285 MS.gene77420:CDS 30.0%
ACAAAAATCTTGTACTGAGT+TGG - chr5.1:30466001-30466020 MS.gene77420:CDS 30.0%
AGAACAAAGAAAGATTGAAG+AGG - chr5.1:30465707-30465726 MS.gene77420:intron 30.0%
AGAAGTTAGCAAAATTGATG+AGG - chr5.1:30464533-30464552 MS.gene77420:CDS 30.0%
AGGAAAAACATGTGAGAAAA+GGG + chr5.1:30466761-30466780 None:intergenic 30.0%
ATAAAATTGCCTTATCCATC+TGG - chr5.1:30465420-30465439 MS.gene77420:CDS 30.0%
ATGGCTAAATCAATTTCTTC+TGG - chr5.1:30464327-30464346 MS.gene77420:CDS 30.0%
CAAGAAAATAAATAAGCTCC+TGG - chr5.1:30466226-30466245 MS.gene77420:CDS 30.0%
CTATTCAATCTCCTAAGTTA+TGG - chr5.1:30466309-30466328 MS.gene77420:CDS 30.0%
GACACTCTGAATAAAATAGA+AGG + chr5.1:30466441-30466460 None:intergenic 30.0%
TATTTATGCCTTTATGATCC+AGG - chr5.1:30466789-30466808 MS.gene77420:CDS 30.0%
TCATAGGCATTTATCATGAT+TGG - chr5.1:30466396-30466415 MS.gene77420:intron 30.0%
TGCATTATAAGTTGTATAGG+TGG - chr5.1:30466953-30466972 MS.gene77420:CDS 30.0%
TTAAGACATCAACTTCATCA+CGG + chr5.1:30467171-30467190 None:intergenic 30.0%
TTGTCATTATGAGTTCCTTA+GGG - chr5.1:30464978-30464997 MS.gene77420:intron 30.0%
TTTGTCATTATGAGTTCCTT+AGG - chr5.1:30464977-30464996 MS.gene77420:intron 30.0%
! AAAGGGAGATCTTTTACAAT+TGG - chr5.1:30464443-30464462 MS.gene77420:CDS 30.0%
! AAATAAGGAAAGTGCATCTT+GGG - chr5.1:30465544-30465563 MS.gene77420:CDS 30.0%
! ACAGCAATTAGTATCTTTTC+AGG + chr5.1:30467268-30467287 None:intergenic 30.0%
! AGATGGATAAGGCAATTTTA+TGG + chr5.1:30465421-30465440 None:intergenic 30.0%
! ATTTTGTCTAGAAAAGGTCA+TGG + chr5.1:30464637-30464656 None:intergenic 30.0%
! TAAATAAGGAAAGTGCATCT+TGG - chr5.1:30465543-30465562 MS.gene77420:CDS 30.0%
! TGAAGTACTTCCAGTAAATA+AGG - chr5.1:30465529-30465548 MS.gene77420:CDS 30.0%
! TGCATTTGATGATTAGATAC+AGG + chr5.1:30467081-30467100 None:intergenic 30.0%
!! TGAAGTTGATGTCTTAATCA+AGG - chr5.1:30467174-30467193 MS.gene77420:CDS 30.0%
AAGTACAGTTTGCACTTGAA+AGG - chr5.1:30465364-30465383 MS.gene77420:CDS 35.0%
AATGGAGTAACATACCCTTT+CGG + chr5.1:30464723-30464742 None:intergenic 35.0%
AATTGCTGTCAAGCAGTATT+GGG - chr5.1:30467279-30467298 MS.gene77420:CDS 35.0%
ACATTCATGACCCATAACTT+AGG + chr5.1:30466323-30466342 None:intergenic 35.0%
AGAACAATGTGCCATATCAA+AGG + chr5.1:30465448-30465467 None:intergenic 35.0%
AGAAGAATGAAATTGCTGTC+TGG - chr5.1:30465763-30465782 MS.gene77420:intron 35.0%
AGAATATGATACAAGCCCTA+AGG + chr5.1:30464996-30465015 None:intergenic 35.0%
AGTACAGTTTGCACTTGAAA+GGG - chr5.1:30465365-30465384 MS.gene77420:CDS 35.0%
ATAGCTGAGAAACATACACT+CGG - chr5.1:30466353-30466372 MS.gene77420:CDS 35.0%
CAATTGGAGGTTACTAAGAA+AGG - chr5.1:30464459-30464478 MS.gene77420:CDS 35.0%
CTACGTATCTGTAATGTGTA+AGG - chr5.1:30465249-30465268 MS.gene77420:CDS 35.0%
GACAGCAATTTCATTCTTCT+CGG + chr5.1:30465763-30465782 None:intergenic 35.0%
GTATGTTTCTCAGCTATAGT+AGG + chr5.1:30466350-30466369 None:intergenic 35.0%
GTGCAGATCTTTATAGTAGT+TGG - chr5.1:30464369-30464388 MS.gene77420:CDS 35.0%
TAATTGCTGTCAAGCAGTAT+TGG - chr5.1:30467278-30467297 MS.gene77420:CDS 35.0%
TATGAGATGGGATACTAAAC+TGG - chr5.1:30467216-30467235 MS.gene77420:CDS 35.0%
TCAACTGTAAGACTCTTGTA+GGG + chr5.1:30464789-30464808 None:intergenic 35.0%
TCGCATATGGAACATAATCA+CGG + chr5.1:30464761-30464780 None:intergenic 35.0%
TCGTTGCAGGTATAAGAAAA+TGG - chr5.1:30466040-30466059 MS.gene77420:CDS 35.0%
TGAACACATTTCCCTCATAT+TGG + chr5.1:30464833-30464852 None:intergenic 35.0%
TTAATTCCTTGGGGTTCAAA+TGG - chr5.1:30465474-30465493 MS.gene77420:CDS 35.0%
TTAGCACAACTTTCTGTGAT+CGG - chr5.1:30466731-30466750 MS.gene77420:CDS 35.0%
TTCTCGTTGCTTAATTCCTT+GGG - chr5.1:30465464-30465483 MS.gene77420:CDS 35.0%
TTGCAAGGCTTAAATACCTT+AGG + chr5.1:30464570-30464589 None:intergenic 35.0%
TTTACGATTAGGAGTAACAC+AGG + chr5.1:30466069-30466088 None:intergenic 35.0%
! AAATTGTTGGAGGTATGAGA+TGG - chr5.1:30467203-30467222 MS.gene77420:CDS 35.0%
! AAGGCAATTTTATGGTTCCT+AGG + chr5.1:30465413-30465432 None:intergenic 35.0%
! AATCTTGTACTGAGTTGGAA+TGG - chr5.1:30466006-30466025 MS.gene77420:CDS 35.0%
! AATTGTTGGAGGTATGAGAT+GGG - chr5.1:30467204-30467223 MS.gene77420:CDS 35.0%
! AGATGATATTCTTCTGGAGA+TGG - chr5.1:30467111-30467130 MS.gene77420:CDS 35.0%
! CGTTTTCATGCAATACATCA+AGG + chr5.1:30466692-30466711 None:intergenic 35.0%
! GATGCACTTTCCTTATTTAC+TGG + chr5.1:30465542-30465561 None:intergenic 35.0%
! TAAGGTATTTAAGCCTTGCA+AGG - chr5.1:30464569-30464588 MS.gene77420:intron 35.0%
! TGAGGTTGATTCAAAACCTA+AGG - chr5.1:30464551-30464570 MS.gene77420:intron 35.0%
! TTAAGCTTTGATTCTCACCA+TGG - chr5.1:30464507-30464526 MS.gene77420:CDS 35.0%
! TTTTGCTAACTTCTTCACCA+TGG + chr5.1:30464527-30464546 None:intergenic 35.0%
!! AAGCTTTTTCCACATATCCA+AGG - chr5.1:30466816-30466835 MS.gene77420:CDS 35.0%
!! AGTATCTTTTCAGGAACAAG+TGG + chr5.1:30467259-30467278 None:intergenic 35.0%
!! ATGGATATGAATGCTGGTTT+GGG - chr5.1:30466272-30466291 MS.gene77420:CDS 35.0%
!! CTTTGTAATTAGCCTTCCAA+TGG + chr5.1:30465656-30465675 None:intergenic 35.0%
!! TAATTAGCCTTCCAATGGAT+TGG + chr5.1:30465651-30465670 None:intergenic 35.0%
AATGAACACAACTGGCAAAG+GGG + chr5.1:30467043-30467062 None:intergenic 40.0%
ACGACCTCATTCATAGCAAT+GGG - chr5.1:30466843-30466862 MS.gene77420:CDS 40.0%
ACTCCTAATCGTAAAGTCCA+CGG - chr5.1:30466074-30466093 MS.gene77420:intron 40.0%
AGAATGAACACAACTGGCAA+AGG + chr5.1:30467045-30467064 None:intergenic 40.0%
ATAAATAAGCTCCTGGACTC+TGG - chr5.1:30466233-30466252 MS.gene77420:CDS 40.0%
ATATATTTGTCAGCCCCTTG+AGG + chr5.1:30464882-30464901 None:intergenic 40.0%
ATTTATCAGCGAGGACTCAT+AGG - chr5.1:30466380-30466399 MS.gene77420:intron 40.0%
CAAGAGTCTTACAGTTGAGA+AGG - chr5.1:30464791-30464810 MS.gene77420:CDS 40.0%
CAGAACTGGATCCAATATGA+GGG - chr5.1:30464819-30464838 MS.gene77420:CDS 40.0%
CGAAGTAGACAAGAAGATTC+TGG - chr5.1:30465813-30465832 MS.gene77420:CDS 40.0%
CTCAACTGTAAGACTCTTGT+AGG + chr5.1:30464790-30464809 None:intergenic 40.0%
CTGACTGTTCCATTCTCTAT+CGG + chr5.1:30464927-30464946 None:intergenic 40.0%
GAATGAACACAACTGGCAAA+GGG + chr5.1:30467044-30467063 None:intergenic 40.0%
GACACAAGAATGAACACAAC+TGG + chr5.1:30467051-30467070 None:intergenic 40.0%
GCATATAGTCTCTGTGGAAA+AGG + chr5.1:30466110-30466129 None:intergenic 40.0%
GGCAGATGGGATTTATATGA+TGG - chr5.1:30465575-30465594 MS.gene77420:CDS 40.0%
GGGTTGTCAAATAGTAGCTA+TGG - chr5.1:30465064-30465083 MS.gene77420:intron 40.0%
GGTATGTTACTCCATTTCCA+TGG - chr5.1:30464727-30464746 MS.gene77420:intron 40.0%
GTAACTTCTCTTCTTCACGA+GGG + chr5.1:30464683-30464702 None:intergenic 40.0%
GTACAGTTTGCACTTGAAAG+GGG - chr5.1:30465366-30465385 MS.gene77420:CDS 40.0%
GTGATGTTAGTCGCAGATTA+TGG + chr5.1:30465211-30465230 None:intergenic 40.0%
GTTCTCGTTGCTTAATTCCT+TGG - chr5.1:30465463-30465482 MS.gene77420:CDS 40.0%
TACGACCTCATTCATAGCAA+TGG - chr5.1:30466842-30466861 MS.gene77420:CDS 40.0%
TACTAAACTGGTTGACCATG+AGG - chr5.1:30467228-30467247 MS.gene77420:CDS 40.0%
TAGCACAACTTTCTGTGATC+GGG - chr5.1:30466732-30466751 MS.gene77420:CDS 40.0%
TATATTTGTCAGCCCCTTGA+GGG + chr5.1:30464881-30464900 None:intergenic 40.0%
TCAGAACTGGATCCAATATG+AGG - chr5.1:30464818-30464837 MS.gene77420:CDS 40.0%
TCTCGTTGCTTAATTCCTTG+GGG - chr5.1:30465465-30465484 MS.gene77420:CDS 40.0%
TCTGTGATACCGATAGAGAA+TGG - chr5.1:30464915-30464934 MS.gene77420:intron 40.0%
TCTTCTACATATTAGGTGCG+TGG - chr5.1:30464403-30464422 MS.gene77420:CDS 40.0%
TGTAACTTCTCTTCTTCACG+AGG + chr5.1:30464684-30464703 None:intergenic 40.0%
TTGAGAAGGCTATTCAGAAC+TGG - chr5.1:30464805-30464824 MS.gene77420:CDS 40.0%
! ACCAGGATGATAACGAAAAG+TGG - chr5.1:30466183-30466202 MS.gene77420:CDS 40.0%
! AGTGCATCTTGGGTTGTTAT+TGG - chr5.1:30465554-30465573 MS.gene77420:CDS 40.0%
! ATATTCTTCTGGAGATGGAC+CGG - chr5.1:30467116-30467135 MS.gene77420:CDS 40.0%
! CAACAGATGCTGATGATGTT+TGG - chr5.1:30465853-30465872 MS.gene77420:CDS 40.0%
! CATGGATATGAATGCTGGTT+TGG - chr5.1:30466271-30466290 MS.gene77420:CDS 40.0%
! GGGAGATCTTTTACAATTGG+AGG - chr5.1:30464446-30464465 MS.gene77420:CDS 40.0%
! TTGGGTTGTTATTGGCAGAT+GGG - chr5.1:30465562-30465581 MS.gene77420:CDS 40.0%
!! AGGTTACTAAGAAAGGTGCT+GGG - chr5.1:30464466-30464485 MS.gene77420:CDS 40.0%
!! TCCACTTTTCGTTATCATCC+TGG + chr5.1:30466187-30466206 None:intergenic 40.0%
!! TGGAAGGTATCGCAATATCA+TGG - chr5.1:30466253-30466272 MS.gene77420:CDS 40.0%
!! TGTGTAAGGTTGTTTTGCAC+AGG - chr5.1:30465263-30465282 MS.gene77420:CDS 40.0%
!!! CAGAGAACTGGTTTTGGAAA+GGG - chr5.1:30464426-30464445 MS.gene77420:CDS 40.0%
!!! GGATTTGTTTTTGTCGTTGC+AGG - chr5.1:30466027-30466046 MS.gene77420:CDS 40.0%
AAACTGGTTGACCATGAGGA+CGG - chr5.1:30467232-30467251 MS.gene77420:CDS 45.0%
AAGACCTAAGGAGGATCTTG+AGG - chr5.1:30465683-30465702 MS.gene77420:intron 45.0%
AATGTGTTCAGATTCCCTGG+TGG - chr5.1:30464843-30464862 MS.gene77420:intron 45.0%
ACAGAACCACTGGCAACTTT+AGG + chr5.1:30466140-30466159 None:intergenic 45.0%
AGAGCCCATTGCTATGAATG+AGG + chr5.1:30466850-30466869 None:intergenic 45.0%
AGGTTGCTAAACTCCTTTGC+TGG - chr5.1:30465727-30465746 MS.gene77420:intron 45.0%
AGTTTCAGCTGAGACATACC+AGG - chr5.1:30466166-30466185 MS.gene77420:CDS 45.0%
CACCTTCAGGTCGTAAAATC+CGG + chr5.1:30467138-30467157 None:intergenic 45.0%
CGATAGAGAATGGAACAGTC+AGG - chr5.1:30464925-30464944 MS.gene77420:intron 45.0%
GAAAAGGCTTAAGATCTCCG+TGG + chr5.1:30466094-30466113 None:intergenic 45.0%
GAGACACTATAGCACTGTAG+TGG - chr5.1:30465163-30465182 MS.gene77420:intron 45.0%
GCTTCACACCTGGATCATAA+AGG + chr5.1:30466800-30466819 None:intergenic 45.0%
GGAAATGTGTTCAGATTCCC+TGG - chr5.1:30464840-30464859 MS.gene77420:intron 45.0%
GGAACATAATCACGGCTCTT+TGG + chr5.1:30464753-30464772 None:intergenic 45.0%
GGAACTGCATATAGTCTCTG+TGG + chr5.1:30466116-30466135 None:intergenic 45.0%
GGAAGGCTAATTACAAAGCC+TGG - chr5.1:30465658-30465677 MS.gene77420:intron 45.0%
GGTTGCTAAACTCCTTTGCT+GGG - chr5.1:30465728-30465747 MS.gene77420:intron 45.0%
GTATGTCTCAGCTGAAACTC+CGG + chr5.1:30466165-30466184 None:intergenic 45.0%
TATGTCTCAGCTGAAACTCC+GGG + chr5.1:30466164-30466183 None:intergenic 45.0%
TCAAAGGCTCCAGATGGATA+AGG + chr5.1:30465432-30465451 None:intergenic 45.0%
TCGATATATCGAGCCTTGCA+AGG + chr5.1:30464585-30464604 None:intergenic 45.0%
TCTCCGTGGACTTTACGATT+AGG + chr5.1:30466080-30466099 None:intergenic 45.0%
TCTTCCTCAAGATCCTCCTT+AGG + chr5.1:30465690-30465709 None:intergenic 45.0%
TGGGCTCTTTAGTCTGTACA+AGG - chr5.1:30466862-30466881 MS.gene77420:CDS 45.0%
TGTGGAAAAAGCTTCACACC+TGG + chr5.1:30466810-30466829 None:intergenic 45.0%
TTCTCACCATTTGAACCCCA+AGG + chr5.1:30465483-30465502 None:intergenic 45.0%
! CGCTATGTCATTTGCACCTA+GGG - chr5.1:30465326-30465345 MS.gene77420:CDS 45.0%
! CTTGGGTTGTTATTGGCAGA+TGG - chr5.1:30465561-30465580 MS.gene77420:CDS 45.0%
! GAGGTTACTAAGAAAGGTGC+TGG - chr5.1:30464465-30464484 MS.gene77420:CDS 45.0%
! GTTTGATACCAGCACCGAAA+GGG - chr5.1:30464706-30464725 MS.gene77420:intron 45.0%
! TCCATCTGGAGCCTTTGATA+TGG - chr5.1:30465434-30465453 MS.gene77420:CDS 45.0%
! TCGCTATGTCATTTGCACCT+AGG - chr5.1:30465325-30465344 MS.gene77420:CDS 45.0%
! TCTAAGACCTGGCGGTTATT+GGG - chr5.1:30465611-30465630 MS.gene77420:intron 45.0%
! TGTTTGATACCAGCACCGAA+AGG - chr5.1:30464705-30464724 MS.gene77420:intron 45.0%
! TTTTGCACAGGTTGCCAGTT+GGG - chr5.1:30465275-30465294 MS.gene77420:CDS 45.0%
!! TTAGCCTTCCAATGGATTGG+AGG + chr5.1:30465648-30465667 None:intergenic 45.0%
!! TTCTAAGACCTGGCGGTTAT+TGG - chr5.1:30465610-30465629 MS.gene77420:intron 45.0%
!!! GCAGAGAACTGGTTTTGGAA+AGG - chr5.1:30464425-30464444 MS.gene77420:CDS 45.0%
AACCGCCAGGTCTTAGAACT+CGG + chr5.1:30465608-30465627 None:intergenic 50.0%
ATAAGCTCCTGGACTCTGGA+AGG - chr5.1:30466237-30466256 MS.gene77420:CDS 50.0%
ATCCTCCTTAGGTCTTTGCC+AGG + chr5.1:30465679-30465698 None:intergenic 50.0%
CAAAGCCTGGCAAAGACCTA+AGG - chr5.1:30465671-30465690 MS.gene77420:intron 50.0%
CCAAAGATATGCACCCCAAC+TGG + chr5.1:30465292-30465311 None:intergenic 50.0%
CCAGTTGGGGTGCATATCTT+TGG - chr5.1:30465289-30465308 MS.gene77420:CDS 50.0%
CGGAATATCACAGCACCTTC+AGG + chr5.1:30467151-30467170 None:intergenic 50.0%
GAACCACTGGCAACTTTAGG+AGG + chr5.1:30466137-30466156 None:intergenic 50.0%
GCCATATCAAAGGCTCCAGA+TGG + chr5.1:30465438-30465457 None:intergenic 50.0%
GGTTCCTAGGACACCAATGA+CGG + chr5.1:30465400-30465419 None:intergenic 50.0%
GTCGTACGTCCTTGGATATG+TGG + chr5.1:30466828-30466847 None:intergenic 50.0%
GTGGAACCCAATTCCCTCAA+GGG - chr5.1:30464865-30464884 MS.gene77420:intron 50.0%
GTTCCTCCTAAAGTTGCCAG+TGG - chr5.1:30466131-30466150 MS.gene77420:intron 50.0%
TAATCACGGCTCTTTGGCCA+TGG + chr5.1:30464747-30464766 None:intergenic 50.0%
TGAATGAGGTCGTACGTCCT+TGG + chr5.1:30466836-30466855 None:intergenic 50.0%
TGGAACCCAATTCCCTCAAG+GGG - chr5.1:30464866-30464885 MS.gene77420:intron 50.0%
TGGCGGTTATTGGGTGCTTT+CGG - chr5.1:30465620-30465639 MS.gene77420:intron 50.0%
TGTGCTTCATGTGAGTCCCT+AGG + chr5.1:30465345-30465364 None:intergenic 50.0%
TTGTAGGGTGCGTTCGCATA+TGG + chr5.1:30464774-30464793 None:intergenic 50.0%
TTTCGGGACCTCCAATCCAT+TGG - chr5.1:30465637-30465656 MS.gene77420:intron 50.0%
TTTGCACAGGTTGCCAGTTG+GGG - chr5.1:30465276-30465295 MS.gene77420:CDS 50.0%
! GACCGGATTTTACGACCTGA+AGG - chr5.1:30467133-30467152 MS.gene77420:CDS 50.0%
! GTAACATACCCTTTCGGTGC+TGG + chr5.1:30464717-30464736 None:intergenic 50.0%
! GTTTTGCACAGGTTGCCAGT+TGG - chr5.1:30465274-30465293 MS.gene77420:CDS 50.0%
!! CTCGGTGTCATTTATCAGCG+AGG - chr5.1:30466371-30466390 MS.gene77420:intron 50.0%
!! GTTGACCGAGTTCTAAGACC+TGG - chr5.1:30465600-30465619 MS.gene77420:intron 50.0%
AACTCCGGGAACAGAACCAC+TGG + chr5.1:30466150-30466169 None:intergenic 55.0%
ACACGCCCTGCCATGATCAA+CGG - chr5.1:30464607-30464626 MS.gene77420:intron 55.0%
AGCCTGGCAAAGACCTAAGG+AGG - chr5.1:30465674-30465693 MS.gene77420:intron 55.0%
CGGCTCTTTGGCCATGGAAA+TGG + chr5.1:30464741-30464760 None:intergenic 55.0%
CGGTCTTCTTCTCCCAGCAA+AGG + chr5.1:30465743-30465762 None:intergenic 55.0%
GAACAAGTGGACCGTCCTCA+TGG + chr5.1:30467246-30467265 None:intergenic 55.0%
GGAATTGGGTTCCACCACCA+GGG + chr5.1:30464860-30464879 None:intergenic 55.0%
GGCGGTTATTGGGTGCTTTC+GGG - chr5.1:30465621-30465640 MS.gene77420:intron 55.0%
GGGACCTCCAATCCATTGGA+AGG - chr5.1:30465641-30465660 MS.gene77420:intron 55.0%
GGTGGAACCCAATTCCCTCA+AGG - chr5.1:30464864-30464883 MS.gene77420:intron 55.0%
GTCAGCCCCTTGAGGGAATT+GGG + chr5.1:30464874-30464893 None:intergenic 55.0%
GTGTTCAGATTCCCTGGTGG+TGG - chr5.1:30464846-30464865 MS.gene77420:intron 55.0%
TCATGGCACGCCGTTGATCA+TGG + chr5.1:30464620-30464639 None:intergenic 55.0%
TGCGATACCTTCCAGAGTCC+AGG + chr5.1:30466247-30466266 None:intergenic 55.0%
TGGAACAGTCAGGACAGCAC+TGG - chr5.1:30464935-30464954 MS.gene77420:intron 55.0%
TGTCAGCCCCTTGAGGGAAT+TGG + chr5.1:30464875-30464894 None:intergenic 55.0%
TTAGGTGCGTGGCAGAGAAC+TGG - chr5.1:30464414-30464433 MS.gene77420:CDS 55.0%
! ACAGCACTGGACACTGGTTG+TGG - chr5.1:30464948-30464967 MS.gene77420:intron 55.0%
! AGCACTGGACACTGGTTGTG+GGG - chr5.1:30464950-30464969 MS.gene77420:intron 55.0%
! CAGCACTGGACACTGGTTGT+GGG - chr5.1:30464949-30464968 MS.gene77420:intron 55.0%
! GTTGCCAGTGGTTCTGTTCC+CGG - chr5.1:30466143-30466162 MS.gene77420:intron 55.0%
!! GAAAGCACCCAATAACCGCC+AGG + chr5.1:30465621-30465640 None:intergenic 55.0%
!! GACCGAGTTCTAAGACCTGG+CGG - chr5.1:30465603-30465622 MS.gene77420:intron 55.0%
!! GCGTGGCAGAGAACTGGTTT+TGG - chr5.1:30464420-30464439 MS.gene77420:CDS 55.0%
CCTAGGACACCAATGACGGC+AGG + chr5.1:30465396-30465415 None:intergenic 60.0%
GCACGCCGTTGATCATGGCA+GGG + chr5.1:30464615-30464634 None:intergenic 60.0%
GGGAATTGGGTTCCACCACC+AGG + chr5.1:30464861-30464880 None:intergenic 60.0%
! AGGGGTGTACCTGCCGTCAT+TGG - chr5.1:30465384-30465403 MS.gene77420:CDS 60.0%
! CCTGCCGTCATTGGTGTCCT+AGG - chr5.1:30465393-30465412 MS.gene77420:CDS 60.0%
! GTCAGGACAGCACTGGACAC+TGG - chr5.1:30464942-30464961 MS.gene77420:intron 60.0%
GGCACGCCGTTGATCATGGC+AGG + chr5.1:30464616-30464635 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 30464327 30467333 30464327 ID=MS.gene77420
chr5.1 mRNA 30464327 30467333 30464327 ID=MS.gene77420.t1;Parent=MS.gene77420
chr5.1 exon 30466689 30467333 30466689 ID=MS.gene77420.t1.exon1;Parent=MS.gene77420.t1
chr5.1 CDS 30466689 30467333 30466689 ID=cds.MS.gene77420.t1;Parent=MS.gene77420.t1
chr5.1 exon 30466165 30466375 30466165 ID=MS.gene77420.t1.exon2;Parent=MS.gene77420.t1
chr5.1 CDS 30466165 30466375 30466165 ID=cds.MS.gene77420.t1;Parent=MS.gene77420.t1
chr5.1 exon 30465786 30466080 30465786 ID=MS.gene77420.t1.exon3;Parent=MS.gene77420.t1
chr5.1 CDS 30465786 30466080 30465786 ID=cds.MS.gene77420.t1;Parent=MS.gene77420.t1
chr5.1 exon 30465243 30465611 30465243 ID=MS.gene77420.t1.exon4;Parent=MS.gene77420.t1
chr5.1 CDS 30465243 30465611 30465243 ID=cds.MS.gene77420.t1;Parent=MS.gene77420.t1
chr5.1 exon 30464772 30464849 30464772 ID=MS.gene77420.t1.exon5;Parent=MS.gene77420.t1
chr5.1 CDS 30464772 30464849 30464772 ID=cds.MS.gene77420.t1;Parent=MS.gene77420.t1
chr5.1 exon 30464327 30464558 30464327 ID=MS.gene77420.t1.exon6;Parent=MS.gene77420.t1
chr5.1 CDS 30464327 30464558 30464327 ID=cds.MS.gene77420.t1;Parent=MS.gene77420.t1
Gene Sequence

>MS.gene77420

ATGGCTAAATCAATTTCTTCTGGTAGTAGAACTAGAAGCTTTGTGCAGATCTTTATAGTAGTTGGTCTATGTTGTTTCTTCTACATATTAGGTGCGTGGCAGAGAACTGGTTTTGGAAAGGGAGATCTTTTACAATTGGAGGTTACTAAGAAAGGTGCTGGGTGTGATATTGTTCCAAATTTAAGCTTTGATTCTCACCATGGTGAAGAAGTTAGCAAAATTGATGAGGTTGATTCAAAACCTAAGGTATTTAAGCCTTGCAAGGCTCGATATATCGATTACACGCCCTGCCATGATCAACGGCGTGCCATGACCTTTTCTAGACAAAATATGATTTATCGAGAGAGGCATTGCCCTCGTGAAGAAGAGAAGTTACATTGTTTGATACCAGCACCGAAAGGGTATGTTACTCCATTTCCATGGCCAAAGAGCCGTGATTATGTTCCATATGCGAACGCACCCTACAAGAGTCTTACAGTTGAGAAGGCTATTCAGAACTGGATCCAATATGAGGGAAATGTGTTCAGATTCCCTGGTGGTGGAACCCAATTCCCTCAAGGGGCTGACAAATATATTGATCAAATTGCTTCTGTGATACCGATAGAGAATGGAACAGTCAGGACAGCACTGGACACTGGTTGTGGGGTACGTTTGTCATTATGAGTTCCTTAGGGCTTGTATCATATTCTTTTGTCATTTTTGTTCATGTTAATTTGATTAGCAGTATATTTAGTTCAGGGTTGTCAAATAGTAGCTATGGTGCTATAGCATAACAGTATTTGAACAAATCGCTATTGCTATGTAATACGCTATATAGTACAAAGTGTTGTCAAATAGAGACACTATAGCACTGTAGTGGAATTTGAACAAACAAATATTTTCCATAATCTGCGACTAACATCACTGAGTTAGTTTGTTTTAGCTACGTATCTGTAATGTGTAAGGTTGTTTTGCACAGGTTGCCAGTTGGGGTGCATATCTTTGGAGCAGAAATGTTATCGCTATGTCATTTGCACCTAGGGACTCACATGAAGCACAAGTACAGTTTGCACTTGAAAGGGGTGTACCTGCCGTCATTGGTGTCCTAGGAACCATAAAATTGCCTTATCCATCTGGAGCCTTTGATATGGCACATTGTTCTCGTTGCTTAATTCCTTGGGGTTCAAATGGTGAGAAATTTACATTAACTATAATTTTTCTTCTGAAGTACTTCCAGTAAATAAGGAAAGTGCATCTTGGGTTGTTATTGGCAGATGGGATTTATATGATGGAAGTTGACCGAGTTCTAAGACCTGGCGGTTATTGGGTGCTTTCGGGACCTCCAATCCATTGGAAGGCTAATTACAAAGCCTGGCAAAGACCTAAGGAGGATCTTGAGGAAGAACAAAGAAAGATTGAAGAGGTTGCTAAACTCCTTTGCTGGGAGAAGAAGACCGAGAAGAATGAAATTGCTGTCTGGCAGAAAACTGTTGATTCTGAAACATGTCGAAGTAGACAAGAAGATTCTGGTGTGAAGTTCTGTGAATCAACAGATGCTGATGATGTTTGGTATGTGCTTTCTTTCAGTGTTAAACATTTATGTATTTCAGCATCCATTGTTGATATTCCATTCATTTAGTTCTACTAACAAACTAGATCATTCGAGCATGCATAAATATAAGAAAAAAGTTGTGTACAAAAATCTTGTACTGAGTTGGAATGGATTTGTTTTTGTCGTTGCAGGTATAAGAAAATGGAAGCCTGTGTTACTCCTAATCGTAAAGTCCACGGAGATCTTAAGCCTTTTCCACAGAGACTATATGCAGTTCCTCCTAAAGTTGCCAGTGGTTCTGTTCCCGGAGTTTCAGCTGAGACATACCAGGATGATAACGAAAAGTGGAAAAAACATGTAAATGCTTACAAGAAAATAAATAAGCTCCTGGACTCTGGAAGGTATCGCAATATCATGGATATGAATGCTGGTTTGGGTAGTTTTGCTGCTGCTATTCAATCTCCTAAGTTATGGGTCATGAATGTTGTGCCTACTATAGCTGAGAAACATACACTCGGTGTCATTTATCAGCGAGGACTCATAGGCATTTATCATGATTGGTATAGTTTTCTAACTTCTTCCTTCTATTTTATTCAGAGTGTCTTGATTTTATGTTTGAAAATGCTTAATCTTGTACTATTATTGTTTTGAGAAATGATACTTTGACATCAAAATTTGATTTCAAATACAGTGTTCTTTGCATTTATGTGTATCTATGAAATGTGTGTAAATTGTAGAACATTAAAAGTAAGAGAAAGAGAGATAGAGATAAACATATACGGTGTCACATACAGTGTCTGTATTTAAATAACACAACTCAATTCTTTTTCTCCTTGATGTATTGCATGAAAACGTAGACTCTATCTTGTATTGTTAGCACAACTTTCTGTGATCGGGTTGCCCTTTTCTCACATGTTTTTCCTTATTTATTTATTTATGCCTTTATGATCCAGGTGTGAAGCTTTTTCCACATATCCAAGGACGTACGACCTCATTCATAGCAATGGGCTCTTTAGTCTGTACAAGGACAAGTGAGTATTCCTTTAATTATATGGTTATTCAATTCTTAATCAATAGTTTAGCCTTTGCATAGAATGCATTATAAGTTGTATAGGTGGTATGAAAATGATATCAAGATAATGATTATCAATGATAGAACCACTTAGTTTTAGTTCTCTCTTTCCCCTTTGCCAGTTGTGTTCATTCTTGTGTCTCAAAATCCTGTATCTAATCATCAAATGCAGATGTAACATAGATGATATTCTTCTGGAGATGGACCGGATTTTACGACCTGAAGGTGCTGTGATATTCCGTGATGAAGTTGATGTCTTAATCAAGGTAAAGAAAATTGTTGGAGGTATGAGATGGGATACTAAACTGGTTGACCATGAGGACGGTCCACTTGTTCCTGAAAAGATACTAATTGCTGTCAAGCAGTATTGGGTTGCTGATACAAATGCCACGTCAACACAATAA
Protein sequence

>MS.gene77420.t1

MAKSISSGSRTRSFVQIFIVVGLCCFFYILGAWQRTGFGKGDLLQLEVTKKGAGCDIVPNLSFDSHHGEEVSKIDEVDSKPKVFKPCKARYIDYTPCHDQRRAMTFSRQNMIYRERHCPREEEKLHCLIPAPKGYVTPFPWPKSRDYVPYANAPYKSLTVEKAIQNWIQYEGNVFRFPGGGTQFPQGADKYIDQIASVIPIENGTVRTALDTGCGVASWGAYLWSRNVIAMSFAPRDSHEAQVQFALERGVPAVIGVLGTIKLPYPSGAFDMAHCSRCLIPWGSNDGIYMMEVDRVLRPGGYWVLSGPPIHWKANYKAWQRPKEDLEEEQRKIEEVAKLLCWEKKTEKNEIAVWQKTVDSETCRSRQEDSGVKFCESTDADDVWYKKMEACVTPNRKVHGDLKPFPQRLYAVPPKVASGSVPGVSAETYQDDNEKWKKHVNAYKKINKLLDSGRYRNIMDMNAGLGSFAAAIQSPKLWVMNVVPTIAEKHTLGVIYQRGLIGIYHDWCEAFSTYPRTYDLIHSNGLFSLYKDKCNIDDILLEMDRILRPEGAVIFRDEVDVLIKVKKIVGGMRWDTKLVDHEDGPLVPEKILIAVKQYWVADTNATSTQ