Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77452.t1 | XP_003601724.1 | 99.4 | 173 | 1 | 0 | 1 | 173 | 1 | 173 | 9.20E-99 | 369.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77452.t1 | Q4PE39 | 46.1 | 167 | 86 | 2 | 8 | 173 | 5 | 168 | 5.5e-44 | 178.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77452.t1 | G7JBC1 | 99.4 | 173 | 1 | 0 | 1 | 173 | 1 | 173 | 6.6e-99 | 369.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050330 | MS.gene77452 | 0.820928 | 5.29E-53 | -1.69E-46 |
| MS.gene050786 | MS.gene77452 | 0.856105 | 4.03E-62 | -1.69E-46 |
| MS.gene050787 | MS.gene77452 | -0.80724 | 5.57E-50 | -1.69E-46 |
| MS.gene052309 | MS.gene77452 | 0.842261 | 2.88E-58 | -1.69E-46 |
| MS.gene052358 | MS.gene77452 | 0.879287 | 1.41E-69 | -1.69E-46 |
| MS.gene052361 | MS.gene77452 | 0.872112 | 4.08E-67 | -1.69E-46 |
| MS.gene053588 | MS.gene77452 | 0.801945 | 7.08E-49 | -1.69E-46 |
| MS.gene054017 | MS.gene77452 | 0.812186 | 4.81E-51 | -1.69E-46 |
| MS.gene055784 | MS.gene77452 | 0.885166 | 1.03E-71 | -1.69E-46 |
| MS.gene055785 | MS.gene77452 | 0.844546 | 7.07E-59 | -1.69E-46 |
| MS.gene056971 | MS.gene77452 | 0.810102 | 1.36E-50 | -1.69E-46 |
| MS.gene057127 | MS.gene77452 | 0.880911 | 3.72E-70 | -1.69E-46 |
| MS.gene061040 | MS.gene77452 | 0.872755 | 2.49E-67 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77452.t1 | MTR_3g084740 | 99.422 | 173 | 1 | 0 | 1 | 173 | 1 | 173 | 7.35e-125 | 367 |
| MS.gene77452.t1 | MTR_3g084740 | 99.422 | 173 | 1 | 0 | 1 | 173 | 1 | 173 | 2.20e-124 | 367 |
| MS.gene77452.t1 | MTR_8g011270 | 60.000 | 165 | 65 | 1 | 8 | 172 | 7 | 170 | 1.92e-64 | 211 |
| MS.gene77452.t1 | MTR_1g105605 | 38.372 | 172 | 98 | 4 | 4 | 172 | 1 | 167 | 6.65e-27 | 106 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77452.t1 | AT3G23660 | 71.667 | 180 | 38 | 2 | 1 | 172 | 1 | 175 | 1.04e-88 | 275 |
| MS.gene77452.t1 | AT3G23660 | 71.667 | 180 | 38 | 2 | 1 | 172 | 1 | 175 | 1.75e-88 | 275 |
| MS.gene77452.t1 | AT1G05520 | 68.085 | 188 | 42 | 3 | 1 | 172 | 1 | 186 | 3.76e-85 | 266 |
| MS.gene77452.t1 | AT4G14160 | 68.156 | 179 | 51 | 1 | 1 | 173 | 1 | 179 | 2.11e-79 | 247 |
| MS.gene77452.t1 | AT4G14160 | 68.539 | 178 | 50 | 1 | 1 | 172 | 1 | 178 | 8.64e-77 | 244 |
| MS.gene77452.t1 | AT4G14160 | 68.539 | 178 | 50 | 1 | 1 | 172 | 1 | 178 | 8.77e-77 | 244 |
| MS.gene77452.t1 | AT2G21630 | 46.746 | 169 | 81 | 5 | 10 | 173 | 8 | 172 | 6.16e-43 | 152 |
| MS.gene77452.t1 | AT5G43670 | 32.057 | 209 | 97 | 3 | 10 | 173 | 7 | 215 | 1.69e-35 | 130 |
Find 57 sgRNAs with CRISPR-Local
Find 68 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATCCTTCGGGACTCAGGTTC+AGG | 0.304450 | 3.4:+79416499 | MS.gene77452:CDS |
| CGTTGTAATGAGGTGGAAAA+TGG | 0.324821 | 3.4:-79416265 | None:intergenic |
| TCGGCTCTTGGTAGAGCAAT+TGG | 0.333563 | 3.4:+79416443 | MS.gene77452:CDS |
| ACAGGGGAAGGAGGAGGATT+AGG | 0.354971 | 3.4:-79416365 | None:intergenic |
| GGAGGATTAGGATCGGTATG+AGG | 0.359326 | 3.4:-79416353 | None:intergenic |
| TTGATTTCACCGCTAAGATC+TGG | 0.369179 | 3.4:+79416216 | MS.gene77452:CDS |
| TTACCTGATAATGCTCTTGT+TGG | 0.375458 | 3.4:+79416470 | MS.gene77452:CDS |
| TGGTTCCGTTGATAACAAAA+TGG | 0.383931 | 3.4:-79416245 | None:intergenic |
| GTGAAATCAACGCGGGAGTA+AGG | 0.395323 | 3.4:-79416203 | None:intergenic |
| AACCCAACAAGAGCATTATC+AGG | 0.413969 | 3.4:-79416473 | None:intergenic |
| TACCTGATAATGCTCTTGTT+GGG | 0.419336 | 3.4:+79416471 | MS.gene77452:CDS |
| ATAAGGAAGACTGGGGATAT+CGG | 0.430962 | 3.4:-79416141 | None:intergenic |
| GGCGAGTGGGATAACGCATT+TGG | 0.433530 | 3.4:-79416090 | None:intergenic |
| CGGCTCTTGGTAGAGCAATT+GGG | 0.438079 | 3.4:+79416444 | MS.gene77452:CDS |
| TCCGTATGACATGGAACGTC+TGG | 0.441775 | 3.4:+79416045 | MS.gene77452:CDS |
| AGGGTTGAGAATGGAGGTGC+AGG | 0.459989 | 3.4:-79416183 | None:intergenic |
| AGAGATTAATTATCTCAAAT+CGG | 0.462983 | 3.4:+79416424 | MS.gene77452:CDS |
| TAAGGAAGACTGGGGATATC+GGG | 0.463197 | 3.4:-79416140 | None:intergenic |
| TATCGGGATGAGAACGGATG+AGG | 0.465331 | 3.4:-79416124 | None:intergenic |
| AATTATCTCAAATCGGCTCT+TGG | 0.465455 | 3.4:+79416431 | MS.gene77452:CDS |
| TTGCAACGGAGGGGTGCATA+AGG | 0.479670 | 3.4:-79416158 | None:intergenic |
| GAGGGGTGCATAAGGAAGAC+TGG | 0.504207 | 3.4:-79416150 | None:intergenic |
| ACGGACTCCGTCGATTCCCT+CGG | 0.506453 | 3.4:-79416027 | None:intergenic |
| ATCATCAACGATGTCGGAGA+TGG | 0.510358 | 3.4:+79415992 | None:intergenic |
| GAGGATTAGGATCGGTATGA+GGG | 0.531440 | 3.4:-79416352 | None:intergenic |
| AGGGAGATTGTGGCGGCGAG+TGG | 0.542010 | 3.4:-79416104 | None:intergenic |
| TCAGAAATCGCGTTGTAATG+AGG | 0.548123 | 3.4:-79416275 | None:intergenic |
| AGGGGTGCATAAGGAAGACT+GGG | 0.548496 | 3.4:-79416149 | None:intergenic |
| CGGACTCCGTCGATTCCCTC+GGG | 0.556274 | 3.4:-79416026 | None:intergenic |
| TATTCAACGGTGGTGTATTG+AGG | 0.556523 | 3.4:-79416323 | None:intergenic |
| GAGTGGGATAACGCATTTGG+AGG | 0.557533 | 3.4:-79416087 | None:intergenic |
| AGAACGGATGAGGGAGATTG+TGG | 0.559381 | 3.4:-79416114 | None:intergenic |
| TATTGAGGATAGAGTTCACC+AGG | 0.561257 | 3.4:-79416308 | None:intergenic |
| GCGGGAGTAAGGGTTGAGAA+TGG | 0.562790 | 3.4:-79416192 | None:intergenic |
| TGAAATCAACGCGGGAGTAA+GGG | 0.568126 | 3.4:-79416202 | None:intergenic |
| ATTTCTGAAACTCATCTCCC+TGG | 0.574744 | 3.4:+79416290 | MS.gene77452:CDS |
| GGGAGATTGTGGCGGCGAGT+GGG | 0.576660 | 3.4:-79416103 | None:intergenic |
| ATCGGGATGAGAACGGATGA+GGG | 0.588713 | 3.4:-79416123 | None:intergenic |
| TCGACGGAGTCCGTATGACA+TGG | 0.594875 | 3.4:+79416036 | MS.gene77452:CDS |
| ACAGATCCCGAGGGAATCGA+CGG | 0.601592 | 3.4:+79416020 | MS.gene77452:CDS |
| TGGGGATATCGGGATGAGAA+CGG | 0.606733 | 3.4:-79416130 | None:intergenic |
| GCCAGACGTTCCATGTCATA+CGG | 0.610591 | 3.4:-79416046 | None:intergenic |
| AACCTGAACCTGAGTCCCGA+AGG | 0.620756 | 3.4:-79416501 | None:intergenic |
| TGGACAGATCCAGATCTTAG+CGG | 0.625634 | 3.4:-79416225 | None:intergenic |
| ATGAGGGATATGATATTCAA+CGG | 0.634959 | 3.4:-79416336 | None:intergenic |
| GATGGCGAACACAGATCCCG+AGG | 0.639336 | 3.4:+79416010 | MS.gene77452:CDS |
| GAAATCGCGTTGTAATGAGG+TGG | 0.643805 | 3.4:-79416272 | None:intergenic |
| GGAGTAAGGGTTGAGAATGG+AGG | 0.671591 | 3.4:-79416189 | None:intergenic |
| CTTAGCGGTGAAATCAACGC+GGG | 0.678250 | 3.4:-79416210 | None:intergenic |
| ACGGATGAGGGAGATTGTGG+CGG | 0.679027 | 3.4:-79416111 | None:intergenic |
| GGAAGGAGGAGGATTAGGAT+CGG | 0.693230 | 3.4:-79416360 | None:intergenic |
| ATGGCGAACACAGATCCCGA+GGG | 0.694002 | 3.4:+79416011 | MS.gene77452:CDS |
| GAGGATTCTACTTTGCTACG+TGG | 0.707824 | 3.4:-79416068 | None:intergenic |
| AGGGATATGATATTCAACGG+TGG | 0.744253 | 3.4:-79416333 | None:intergenic |
| TCTTAGCGGTGAAATCAACG+CGG | 0.800179 | 3.4:-79416211 | None:intergenic |
| ATTGAGGATAGAGTTCACCA+GGG | 0.804613 | 3.4:-79416307 | None:intergenic |
| GGGGTGCATAAGGAAGACTG+GGG | 0.813434 | 3.4:-79416148 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AGAGATTAATTATCTCAAAT+CGG | + | chr3.4:79416424-79416443 | MS.gene77452:CDS | 20.0% |
| ATGAGGGATATGATATTCAA+CGG | - | chr3.4:79416339-79416358 | None:intergenic | 30.0% | |
| !! | TCTGTCCATTTTGTTATCAA+CGG | + | chr3.4:79416240-79416259 | MS.gene77452:CDS | 30.0% |
| AAGCAGAAAGAGAAAAACAG+GGG | - | chr3.4:79416384-79416403 | None:intergenic | 35.0% | |
| CAAGCAGAAAGAGAAAAACA+GGG | - | chr3.4:79416385-79416404 | None:intergenic | 35.0% | |
| TCAAGCAGAAAGAGAAAAAC+AGG | - | chr3.4:79416386-79416405 | None:intergenic | 35.0% | |
| ! | AATTATCTCAAATCGGCTCT+TGG | + | chr3.4:79416431-79416450 | MS.gene77452:CDS | 35.0% |
| ! | CTTGTTGGGTTTTTATCCTT+CGG | + | chr3.4:79416485-79416504 | MS.gene77452:CDS | 35.0% |
| ! | TACCTGATAATGCTCTTGTT+GGG | + | chr3.4:79416471-79416490 | MS.gene77452:CDS | 35.0% |
| ! | TTACCTGATAATGCTCTTGT+TGG | + | chr3.4:79416470-79416489 | MS.gene77452:CDS | 35.0% |
| ! | TTGTTGGGTTTTTATCCTTC+GGG | + | chr3.4:79416486-79416505 | MS.gene77452:CDS | 35.0% |
| !! | TGGTTCCGTTGATAACAAAA+TGG | - | chr3.4:79416248-79416267 | None:intergenic | 35.0% |
| AGAAAGAGAAAAACAGGGGA+AGG | - | chr3.4:79416380-79416399 | None:intergenic | 40.0% | |
| AGGGATATGATATTCAACGG+TGG | - | chr3.4:79416336-79416355 | None:intergenic | 40.0% | |
| ATAAGGAAGACTGGGGATAT+CGG | - | chr3.4:79416144-79416163 | None:intergenic | 40.0% | |
| ATTGAGGATAGAGTTCACCA+GGG | - | chr3.4:79416310-79416329 | None:intergenic | 40.0% | |
| CGTTGTAATGAGGTGGAAAA+TGG | - | chr3.4:79416268-79416287 | None:intergenic | 40.0% | |
| TATTCAACGGTGGTGTATTG+AGG | - | chr3.4:79416326-79416345 | None:intergenic | 40.0% | |
| TATTGAGGATAGAGTTCACC+AGG | - | chr3.4:79416311-79416330 | None:intergenic | 40.0% | |
| TCAGAAATCGCGTTGTAATG+AGG | - | chr3.4:79416278-79416297 | None:intergenic | 40.0% | |
| TTGATTTCACCGCTAAGATC+TGG | + | chr3.4:79416216-79416235 | MS.gene77452:CDS | 40.0% | |
| !! | AACCCAACAAGAGCATTATC+AGG | - | chr3.4:79416476-79416495 | None:intergenic | 40.0% |
| !! | ATTTCTGAAACTCATCTCCC+TGG | + | chr3.4:79416290-79416309 | MS.gene77452:CDS | 40.0% |
| AAGAGAAAAACAGGGGAAGG+AGG | - | chr3.4:79416377-79416396 | None:intergenic | 45.0% | |
| GAAATCGCGTTGTAATGAGG+TGG | - | chr3.4:79416275-79416294 | None:intergenic | 45.0% | |
| GAGGATTAGGATCGGTATGA+GGG | - | chr3.4:79416355-79416374 | None:intergenic | 45.0% | |
| GAGGATTCTACTTTGCTACG+TGG | - | chr3.4:79416071-79416090 | None:intergenic | 45.0% | |
| TAAGGAAGACTGGGGATATC+GGG | - | chr3.4:79416143-79416162 | None:intergenic | 45.0% | |
| TCTTAGCGGTGAAATCAACG+CGG | - | chr3.4:79416214-79416233 | None:intergenic | 45.0% | |
| TGAAATCAACGCGGGAGTAA+GGG | - | chr3.4:79416205-79416224 | None:intergenic | 45.0% | |
| TGGACAGATCCAGATCTTAG+CGG | - | chr3.4:79416228-79416247 | None:intergenic | 45.0% | |
| ! | GTTTTTATCCTTCGGGACTC+AGG | + | chr3.4:79416493-79416512 | MS.gene77452:CDS | 45.0% |
| AGAAAAACAGGGGAAGGAGG+AGG | - | chr3.4:79416374-79416393 | None:intergenic | 50.0% | |
| AGAACGGATGAGGGAGATTG+TGG | - | chr3.4:79416117-79416136 | None:intergenic | 50.0% | |
| ATCGGGATGAGAACGGATGA+GGG | - | chr3.4:79416126-79416145 | None:intergenic | 50.0% | |
| CGGCTCTTGGTAGAGCAATT+GGG | + | chr3.4:79416444-79416463 | MS.gene77452:CDS | 50.0% | |
| CTTAGCGGTGAAATCAACGC+GGG | - | chr3.4:79416213-79416232 | None:intergenic | 50.0% | |
| GAGTGGGATAACGCATTTGG+AGG | - | chr3.4:79416090-79416109 | None:intergenic | 50.0% | |
| GCCAGACGTTCCATGTCATA+CGG | - | chr3.4:79416049-79416068 | None:intergenic | 50.0% | |
| GGAAGGAGGAGGATTAGGAT+CGG | - | chr3.4:79416363-79416382 | None:intergenic | 50.0% | |
| GGAGGATTAGGATCGGTATG+AGG | - | chr3.4:79416356-79416375 | None:intergenic | 50.0% | |
| GGAGTAAGGGTTGAGAATGG+AGG | - | chr3.4:79416192-79416211 | None:intergenic | 50.0% | |
| GTGAAATCAACGCGGGAGTA+AGG | - | chr3.4:79416206-79416225 | None:intergenic | 50.0% | |
| TATCGGGATGAGAACGGATG+AGG | - | chr3.4:79416127-79416146 | None:intergenic | 50.0% | |
| TCCGTATGACATGGAACGTC+TGG | + | chr3.4:79416045-79416064 | MS.gene77452:CDS | 50.0% | |
| TCGGCTCTTGGTAGAGCAAT+TGG | + | chr3.4:79416443-79416462 | MS.gene77452:CDS | 50.0% | |
| TGGGGATATCGGGATGAGAA+CGG | - | chr3.4:79416133-79416152 | None:intergenic | 50.0% | |
| ! | AGGGGTGCATAAGGAAGACT+GGG | - | chr3.4:79416152-79416171 | None:intergenic | 50.0% |
| !! | TGGAGGTGCAGGTTTTGCAA+CGG | - | chr3.4:79416175-79416194 | None:intergenic | 50.0% |
| ACAGATCCCGAGGGAATCGA+CGG | + | chr3.4:79416020-79416039 | MS.gene77452:CDS | 55.0% | |
| ACAGGGGAAGGAGGAGGATT+AGG | - | chr3.4:79416368-79416387 | None:intergenic | 55.0% | |
| ACGGATGAGGGAGATTGTGG+CGG | - | chr3.4:79416114-79416133 | None:intergenic | 55.0% | |
| ATGGCGAACACAGATCCCGA+GGG | + | chr3.4:79416011-79416030 | MS.gene77452:CDS | 55.0% | |
| GCGGGAGTAAGGGTTGAGAA+TGG | - | chr3.4:79416195-79416214 | None:intergenic | 55.0% | |
| GGCGAGTGGGATAACGCATT+TGG | - | chr3.4:79416093-79416112 | None:intergenic | 55.0% | |
| GGGGTGCATAAGGAAGACTG+GGG | - | chr3.4:79416151-79416170 | None:intergenic | 55.0% | |
| TCGACGGAGTCCGTATGACA+TGG | + | chr3.4:79416036-79416055 | MS.gene77452:CDS | 55.0% | |
| ! | AGGGTTGAGAATGGAGGTGC+AGG | - | chr3.4:79416186-79416205 | None:intergenic | 55.0% |
| ! | AGGTGCAGGTTTTGCAACGG+AGG | - | chr3.4:79416172-79416191 | None:intergenic | 55.0% |
| ! | GAGGGGTGCATAAGGAAGAC+TGG | - | chr3.4:79416153-79416172 | None:intergenic | 55.0% |
| ! | GGTGCAGGTTTTGCAACGGA+GGG | - | chr3.4:79416171-79416190 | None:intergenic | 55.0% |
| ! | GTGCAGGTTTTGCAACGGAG+GGG | - | chr3.4:79416170-79416189 | None:intergenic | 55.0% |
| ! | TTGCAACGGAGGGGTGCATA+AGG | - | chr3.4:79416161-79416180 | None:intergenic | 55.0% |
| GATGGCGAACACAGATCCCG+AGG | + | chr3.4:79416010-79416029 | MS.gene77452:CDS | 60.0% | |
| !! | ACGGACTCCGTCGATTCCCT+CGG | - | chr3.4:79416030-79416049 | None:intergenic | 60.0% |
| AGGGAGATTGTGGCGGCGAG+TGG | - | chr3.4:79416107-79416126 | None:intergenic | 65.0% | |
| GGGAGATTGTGGCGGCGAGT+GGG | - | chr3.4:79416106-79416125 | None:intergenic | 65.0% | |
| !! | CGGACTCCGTCGATTCCCTC+GGG | - | chr3.4:79416029-79416048 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 79416002 | 79416520 | 79416002 | ID=MS.gene77452 |
| chr3.4 | mRNA | 79416002 | 79416520 | 79416002 | ID=MS.gene77452.t1;Parent=MS.gene77452 |
| chr3.4 | exon | 79416002 | 79416520 | 79416002 | ID=MS.gene77452.t1.exon1;Parent=MS.gene77452.t1 |
| chr3.4 | CDS | 79416002 | 79416520 | 79416002 | ID=cds.MS.gene77452.t1;Parent=MS.gene77452.t1 |
| Gene Sequence |
| Protein sequence |
