Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene78483.t1 | XP_003626318.2 | 86.8 | 333 | 13 | 5 | 1 | 324 | 6 | 316 | 1.40E-145 | 525.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene78483.t1 | Q9SK91 | 44.3 | 325 | 145 | 8 | 9 | 318 | 1 | 304 | 1.7e-54 | 214.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene78483.t1 | G7KZP3 | 86.8 | 333 | 13 | 5 | 1 | 324 | 6 | 316 | 1.0e-145 | 525.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene78483 | 0.806563 | 7.74E-50 | -1.69E-46 |
| MS.gene049350 | MS.gene78483 | 0.818439 | 1.96E-52 | -1.69E-46 |
| MS.gene049711 | MS.gene78483 | 0.824903 | 6.27E-54 | -1.69E-46 |
| MS.gene051018 | MS.gene78483 | 0.801655 | 8.13E-49 | -1.69E-46 |
| MS.gene051720 | MS.gene78483 | 0.806698 | 7.25E-50 | -1.69E-46 |
| MS.gene052463 | MS.gene78483 | 0.814213 | 1.73E-51 | -1.69E-46 |
| MS.gene05458 | MS.gene78483 | 0.801449 | 8.96E-49 | -1.69E-46 |
| MS.gene054991 | MS.gene78483 | 0.803606 | 3.22E-49 | -1.69E-46 |
| MS.gene055537 | MS.gene78483 | 0.800133 | 1.66E-48 | -1.69E-46 |
| MS.gene057102 | MS.gene78483 | 0.807332 | 5.32E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 43 sgRNAs with CRISPR-Local
Find 71 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGAATTATGCTTATGGAAAT+TGG | 0.223168 | 7.1:-2715068 | MS.gene78483:CDS |
| ACCATCTTCAAGAGCTGTTT+TGG | 0.261447 | 7.1:+2714343 | None:intergenic |
| GGTGATCAAGCATCAATTAT+TGG | 0.265730 | 7.1:-2714619 | MS.gene78483:CDS |
| GTTGAATTCTTTCCAACATT+TGG | 0.301786 | 7.1:+2714017 | None:intergenic |
| GAGCAGAAGCTACAATTTCT+TGG | 0.311027 | 7.1:-2714568 | MS.gene78483:CDS |
| GTGATCAAGCATCAATTATT+GGG | 0.314095 | 7.1:-2714618 | MS.gene78483:CDS |
| CATCAGCTGTAGTGACATTA+AGG | 0.320979 | 7.1:+2714284 | None:intergenic |
| ACCCTTTGGACGTAAGATTC+AGG | 0.337296 | 7.1:+2714805 | None:intergenic |
| TTGCTTCAGAATCTGTTAAC+AGG | 0.346873 | 7.1:+2715166 | None:intergenic |
| CAAACAGAGAATTATGCTTA+TGG | 0.362320 | 7.1:-2715075 | MS.gene78483:CDS |
| CTTCTATTCTATCAGCGTTA+AGG | 0.373348 | 7.1:-2714254 | MS.gene78483:intron |
| AAGCATAATTCTCTGTTTGT+TGG | 0.375728 | 7.1:+2715079 | None:intergenic |
| GTTGAAGATGATTGCAAGCT+TGG | 0.385709 | 7.1:-2714070 | MS.gene78483:CDS |
| ACAATCATTGTTGCCACAAA+TGG | 0.405629 | 7.1:-2715010 | MS.gene78483:CDS |
| CCACAAGACCCTTTCACCTA+TGG | 0.431613 | 7.1:-2715216 | MS.gene78483:CDS |
| TTAACAGGTTGTAATTGTAA+TGG | 0.451750 | 7.1:+2715181 | None:intergenic |
| ACCAAAACAGCTCTTGAAGA+TGG | 0.460812 | 7.1:-2714344 | MS.gene78483:CDS |
| ACATTGCTGTAGAACGCAAC+AGG | 0.469202 | 7.1:-2714873 | MS.gene78483:CDS |
| TTCTTACTTTGAGATTTGCA+TGG | 0.473698 | 7.1:+2714377 | None:intergenic |
| GAAATTGAACTATCTAAAGA+TGG | 0.481389 | 7.1:+2714973 | None:intergenic |
| TGTTGAATCCATAGGTGAAA+GGG | 0.493303 | 7.1:+2715208 | None:intergenic |
| AAGATGGTTTCTAGTTTGCA+TGG | 0.504712 | 7.1:-2714328 | MS.gene78483:CDS |
| GGCAACAATGATTGTGGTGG+TGG | 0.505665 | 7.1:+2715018 | None:intergenic |
| TTTGACACTTCATCCATTTG+TGG | 0.511454 | 7.1:+2714997 | None:intergenic |
| TGCCTGAATCTTACGTCCAA+AGG | 0.512531 | 7.1:-2714807 | MS.gene78483:CDS |
| GCCTGAATCTTACGTCCAAA+GGG | 0.523230 | 7.1:-2714806 | MS.gene78483:intron |
| TCTTGTGGATATAGTAGAGT+TGG | 0.525531 | 7.1:+2715231 | None:intergenic |
| AACAATGATTGTGGTGGTGG+TGG | 0.531255 | 7.1:+2715021 | None:intergenic |
| CTGTCACTTGTTGAGTATTG+TGG | 0.536473 | 7.1:+2714484 | None:intergenic |
| TTGTTGAATCCATAGGTGAA+AGG | 0.539793 | 7.1:+2715207 | None:intergenic |
| TAGAGTTGGTTCTAGTGCCA+TGG | 0.564316 | 7.1:+2715245 | None:intergenic |
| CCATAGGTGAAAGGGTCTTG+TGG | 0.567251 | 7.1:+2715216 | None:intergenic |
| ATGGTCTTTGTTGAATCCAT+AGG | 0.572517 | 7.1:+2715200 | None:intergenic |
| TGCAGCTGTATACCAAATGT+TGG | 0.583918 | 7.1:-2714029 | MS.gene78483:CDS |
| TGATTGCAAGCTTGGATCAG+TGG | 0.587540 | 7.1:-2714062 | MS.gene78483:CDS |
| CAGAAGCTACAATTTCTTGG+TGG | 0.593039 | 7.1:-2714565 | MS.gene78483:CDS |
| TGATCAAGCATCAATTATTG+GGG | 0.599410 | 7.1:-2714617 | MS.gene78483:CDS |
| TAGAGAAAGAGAGAGATCCA+TGG | 0.615999 | 7.1:-2715262 | MS.gene78483:CDS |
| TGCTGATATTGAAGTGACAA+TGG | 0.646131 | 7.1:-2714407 | MS.gene78483:CDS |
| ATTTGTGGCAACAATGATTG+TGG | 0.649128 | 7.1:+2715012 | None:intergenic |
| ACTTGTTGAGTATTGTGGAA+AGG | 0.653314 | 7.1:+2714489 | None:intergenic |
| TGTGGCAACAATGATTGTGG+TGG | 0.680500 | 7.1:+2715015 | None:intergenic |
| GATCAAGCATCAATTATTGG+GGG | 0.757063 | 7.1:-2714616 | MS.gene78483:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATTTTATTTTGTAATTTGT+AGG | - | chr7.1:2715165-2715184 | MS.gene78483:CDS | 10.0% |
| !! | CAATCAATATATTGAATTAA+AGG | - | chr7.1:2715272-2715291 | MS.gene78483:CDS | 15.0% |
| !!! | TCATTTTTAAATTGAAATTG+CGG | + | chr7.1:2714590-2714609 | None:intergenic | 15.0% |
| !! | AAGAAAAAAAAAACATGACA+GGG | + | chr7.1:2715043-2715062 | None:intergenic | 20.0% |
| !! | TTAAGTGCTTACAATAATTT+AGG | + | chr7.1:2715118-2715137 | None:intergenic | 20.0% |
| !!! | CTTCTATTACTTTTAAATTG+TGG | - | chr7.1:2714476-2714495 | MS.gene78483:CDS | 20.0% |
| !!! | TTTGATTGATGAATTTTTCA+GGG | - | chr7.1:2714617-2714636 | MS.gene78483:CDS | 20.0% |
| !!! | TTTTGATTGATGAATTTTTC+AGG | - | chr7.1:2714616-2714635 | MS.gene78483:CDS | 20.0% |
| ! | AAAGGTGAAAAATTCAGAAA+AGG | + | chr7.1:2714753-2714772 | None:intergenic | 25.0% |
| ! | AAGGTGAAAAATTCAGAAAA+GGG | + | chr7.1:2714752-2714771 | None:intergenic | 25.0% |
| ! | AGAATTATGCTTATGGAAAT+TGG | - | chr7.1:2714189-2714208 | MS.gene78483:intron | 25.0% |
| ! | GAAATTGAACTATCTAAAGA+TGG | + | chr7.1:2714287-2714306 | None:intergenic | 25.0% |
| ! | GAAGAAAAAAAAAACATGAC+AGG | + | chr7.1:2715044-2715063 | None:intergenic | 25.0% |
| ! | TTAACAGGTTGTAATTGTAA+TGG | + | chr7.1:2714079-2714098 | None:intergenic | 25.0% |
| !! | AAAGTTCTTCTTGATTTTGA+AGG | + | chr7.1:2714139-2714158 | None:intergenic | 25.0% |
| AAGCATAATTCTCTGTTTGT+TGG | + | chr7.1:2714181-2714200 | None:intergenic | 30.0% | |
| CAAACAGAGAATTATGCTTA+TGG | - | chr7.1:2714182-2714201 | MS.gene78483:intron | 30.0% | |
| GCTAAAAAGTGGTTAGAATT+TGG | + | chr7.1:2715073-2715092 | None:intergenic | 30.0% | |
| GTGATCAAGCATCAATTATT+GGG | - | chr7.1:2714639-2714658 | MS.gene78483:intron | 30.0% | |
| GTTGAATTCTTTCCAACATT+TGG | + | chr7.1:2715243-2715262 | None:intergenic | 30.0% | |
| TGATCAAGCATCAATTATTG+GGG | - | chr7.1:2714640-2714659 | MS.gene78483:intron | 30.0% | |
| TTCAAGAGAAAGCTAAAAAG+TGG | + | chr7.1:2715084-2715103 | None:intergenic | 30.0% | |
| ! | TGCATTTTGCCTCAATATAA+AGG | + | chr7.1:2714524-2714543 | None:intergenic | 30.0% |
| !! | AAAGTAATAGAAGTACCCTT+TGG | + | chr7.1:2714469-2714488 | None:intergenic | 30.0% |
| !! | TTCTTACTTTGAGATTTGCA+TGG | + | chr7.1:2714883-2714902 | None:intergenic | 30.0% |
| !! | TTGATTTTGAAGGTTGAAGT+TGG | + | chr7.1:2714129-2714148 | None:intergenic | 30.0% |
| ACAATCATTGTTGCCACAAA+TGG | - | chr7.1:2714247-2714266 | MS.gene78483:intron | 35.0% | |
| AGTGGTTAGAATTTGGAAAG+TGG | + | chr7.1:2715066-2715085 | None:intergenic | 35.0% | |
| ATGGTCTTTGTTGAATCCAT+AGG | + | chr7.1:2714060-2714079 | None:intergenic | 35.0% | |
| ATTTGTGGCAACAATGATTG+TGG | + | chr7.1:2714248-2714267 | None:intergenic | 35.0% | |
| CTTCTATTCTATCAGCGTTA+AGG | - | chr7.1:2715003-2715022 | MS.gene78483:CDS | 35.0% | |
| GATCAAGCATCAATTATTGG+GGG | - | chr7.1:2714641-2714660 | MS.gene78483:intron | 35.0% | |
| GGTGATCAAGCATCAATTAT+TGG | - | chr7.1:2714638-2714657 | MS.gene78483:intron | 35.0% | |
| TCTTGTGGATATAGTAGAGT+TGG | + | chr7.1:2714029-2714048 | None:intergenic | 35.0% | |
| TGCTGATATTGAAGTGACAA+TGG | - | chr7.1:2714850-2714869 | MS.gene78483:CDS | 35.0% | |
| TGCTGCAAACCTTTATATTG+AGG | - | chr7.1:2714512-2714531 | MS.gene78483:CDS | 35.0% | |
| TGGTGTTCAGAAACAAAAAG+AGG | - | chr7.1:2714712-2714731 | MS.gene78483:intron | 35.0% | |
| TGTTGAATCCATAGGTGAAA+GGG | + | chr7.1:2714052-2714071 | None:intergenic | 35.0% | |
| TTGCTTCAGAATCTGTTAAC+AGG | + | chr7.1:2714094-2714113 | None:intergenic | 35.0% | |
| TTGTTGAATCCATAGGTGAA+AGG | + | chr7.1:2714053-2714072 | None:intergenic | 35.0% | |
| TTTGACACTTCATCCATTTG+TGG | + | chr7.1:2714263-2714282 | None:intergenic | 35.0% | |
| ! | ACTTGTTGAGTATTGTGGAA+AGG | + | chr7.1:2714771-2714790 | None:intergenic | 35.0% |
| !! | AAGATGGTTTCTAGTTTGCA+TGG | - | chr7.1:2714929-2714948 | MS.gene78483:CDS | 35.0% |
| !! | GAGAGTTCTGAGACAAAAAT+GGG | - | chr7.1:2714803-2714822 | MS.gene78483:intron | 35.0% |
| !! | TGAGAGTTCTGAGACAAAAA+TGG | - | chr7.1:2714802-2714821 | MS.gene78483:intron | 35.0% |
| ACCAAAACAGCTCTTGAAGA+TGG | - | chr7.1:2714913-2714932 | MS.gene78483:CDS | 40.0% | |
| AGCGTTAAGGTAATTGTCTG+TGG | - | chr7.1:2715016-2715035 | MS.gene78483:CDS | 40.0% | |
| CAGAAGCTACAATTTCTTGG+TGG | - | chr7.1:2714692-2714711 | MS.gene78483:intron | 40.0% | |
| CATCAGCTGTAGTGACATTA+AGG | + | chr7.1:2714976-2714995 | None:intergenic | 40.0% | |
| CTGCAAGAGAAACGATTCTA+AGG | + | chr7.1:2714567-2714586 | None:intergenic | 40.0% | |
| GAGCAGAAGCTACAATTTCT+TGG | - | chr7.1:2714689-2714708 | MS.gene78483:intron | 40.0% | |
| GAGGCAAAATGCAGAAATTG+CGG | - | chr7.1:2714531-2714550 | MS.gene78483:CDS | 40.0% | |
| GTTGAAGATGATTGCAAGCT+TGG | - | chr7.1:2715187-2715206 | MS.gene78483:CDS | 40.0% | |
| TAGAGAAAGAGAGAGATCCA+TGG | - | chr7.1:2713995-2714014 | MS.gene78483:CDS | 40.0% | |
| TGCAGCTGTATACCAAATGT+TGG | - | chr7.1:2715228-2715247 | MS.gene78483:CDS | 40.0% | |
| ! | ACCATCTTCAAGAGCTGTTT+TGG | + | chr7.1:2714917-2714936 | None:intergenic | 40.0% |
| ! | CTGTCACTTGTTGAGTATTG+TGG | + | chr7.1:2714776-2714795 | None:intergenic | 40.0% |
| !! | AGTTCTGAGACAAAAATGGG+TGG | - | chr7.1:2714806-2714825 | MS.gene78483:intron | 40.0% |
| !!! | TGTTTTGACTCTGCGTTTCT+TGG | + | chr7.1:2714324-2714343 | None:intergenic | 40.0% |
| ACATTGCTGTAGAACGCAAC+AGG | - | chr7.1:2714384-2714403 | MS.gene78483:CDS | 45.0% | |
| ACCCTTTGGACGTAAGATTC+AGG | + | chr7.1:2714455-2714474 | None:intergenic | 45.0% | |
| GCCTGAATCTTACGTCCAAA+GGG | - | chr7.1:2714451-2714470 | MS.gene78483:CDS | 45.0% | |
| TGAGACAAAAATGGGTGGTG+AGG | - | chr7.1:2714811-2714830 | MS.gene78483:CDS | 45.0% | |
| TGATTGCAAGCTTGGATCAG+TGG | - | chr7.1:2715195-2715214 | MS.gene78483:CDS | 45.0% | |
| TGCCTGAATCTTACGTCCAA+AGG | - | chr7.1:2714450-2714469 | MS.gene78483:CDS | 45.0% | |
| TGTGGCAACAATGATTGTGG+TGG | + | chr7.1:2714245-2714264 | None:intergenic | 45.0% | |
| !! | AACAATGATTGTGGTGGTGG+TGG | + | chr7.1:2714239-2714258 | None:intergenic | 45.0% |
| !! | TAGAGTTGGTTCTAGTGCCA+TGG | + | chr7.1:2714015-2714034 | None:intergenic | 45.0% |
| CCACAAGACCCTTTCACCTA+TGG | - | chr7.1:2714041-2714060 | MS.gene78483:CDS | 50.0% | |
| CCATAGGTGAAAGGGTCTTG+TGG | + | chr7.1:2714044-2714063 | None:intergenic | 50.0% | |
| GGCAACAATGATTGTGGTGG+TGG | + | chr7.1:2714242-2714261 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 2713988 | 2715291 | 2713988 | ID=MS.gene78483 |
| chr7.1 | mRNA | 2713988 | 2715291 | 2713988 | ID=MS.gene78483.t1;Parent=MS.gene78483 |
| chr7.1 | exon | 2714807 | 2715291 | 2714807 | ID=MS.gene78483.t1.exon1;Parent=MS.gene78483.t1 |
| chr7.1 | CDS | 2714807 | 2715291 | 2714807 | ID=cds.MS.gene78483.t1;Parent=MS.gene78483.t1 |
| chr7.1 | exon | 2714255 | 2714641 | 2714255 | ID=MS.gene78483.t1.exon2;Parent=MS.gene78483.t1 |
| chr7.1 | CDS | 2714255 | 2714641 | 2714255 | ID=cds.MS.gene78483.t1;Parent=MS.gene78483.t1 |
| chr7.1 | exon | 2713988 | 2714092 | 2713988 | ID=MS.gene78483.t1.exon3;Parent=MS.gene78483.t1 |
| chr7.1 | CDS | 2713988 | 2714092 | 2713988 | ID=cds.MS.gene78483.t1;Parent=MS.gene78483.t1 |
| Gene Sequence |
| Protein sequence |
