Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene78546.t1 | XP_003616262.1 | 94 | 184 | 10 | 1 | 1 | 184 | 1 | 183 | 5.40E-89 | 337 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene78546.t1 | G7KG33 | 94.0 | 184 | 10 | 1 | 1 | 184 | 1 | 183 | 3.9e-89 | 337.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050642 | MS.gene78546 | 0.801156 | 1.03E-48 | -1.69E-46 |
| MS.gene051983 | MS.gene78546 | 0.806188 | 9.28E-50 | -1.69E-46 |
| MS.gene052077 | MS.gene78546 | 0.806315 | 8.73E-50 | -1.69E-46 |
| MS.gene056979 | MS.gene78546 | 0.807232 | 5.59E-50 | -1.69E-46 |
| MS.gene057754 | MS.gene78546 | 0.831136 | 1.98E-55 | -1.69E-46 |
| MS.gene059285 | MS.gene78546 | 0.814158 | 1.78E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene78546.t1 | MTR_5g077940 | 94.022 | 184 | 10 | 1 | 1 | 184 | 1 | 183 | 4.56e-111 | 314 |
| MS.gene78546.t1 | MTR_3g065210 | 39.444 | 180 | 87 | 6 | 1 | 168 | 1 | 170 | 7.57e-26 | 98.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 34 sgRNAs with CRISPR-Local
Find 34 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTGCTAAGGGTTATAAATA+AGG | 0.187915 | 5.1:-60441104 | None:intergenic |
| GCATGGCATCAACGTGGTTC+TGG | 0.325316 | 5.1:-60441524 | MS.gene78546:CDS |
| CTGCTAAGGGTTATAAATAA+GGG | 0.356986 | 5.1:-60441103 | None:intergenic |
| GTGTGGAGGATGGTGTTAAA+AGG | 0.375850 | 5.1:-60441256 | MS.gene78546:CDS |
| CCATGCAGCTGCTTTCACTA+TGG | 0.411092 | 5.1:+60441547 | None:intergenic |
| AAGAAGAAGAAGATAAAGAA+AGG | 0.424390 | 5.1:-60441230 | MS.gene78546:CDS |
| AGAGTTTAGAAATGAGAGCT+TGG | 0.426380 | 5.1:+60441631 | None:intergenic |
| ATGAAGTTCAAAGCATTTCA+AGG | 0.433722 | 5.1:-60441343 | MS.gene78546:CDS |
| TAAGAGTGTACCTTCTGCTA+AGG | 0.486987 | 5.1:-60441117 | MS.gene78546:CDS |
| AGCAGTGAAATCAATGGAAA+AGG | 0.488934 | 5.1:-60441405 | MS.gene78546:CDS |
| TGGTGTTGTATGTGGAAGAT+TGG | 0.490879 | 5.1:-60441192 | MS.gene78546:CDS |
| CACAAGCATGGTGTTGTATG+TGG | 0.492459 | 5.1:-60441200 | MS.gene78546:CDS |
| AGAAGAAGAAGATAAAGAAA+GGG | 0.496208 | 5.1:-60441229 | MS.gene78546:CDS |
| TTGATCCAACTAGTGTTTCA+AGG | 0.497723 | 5.1:-60441160 | MS.gene78546:CDS |
| ATTAGAAGCAGTGAAATCAA+TGG | 0.505959 | 5.1:-60441411 | MS.gene78546:CDS |
| CATAGTGAAAGCAGCTGCAT+GGG | 0.510979 | 5.1:-60441546 | MS.gene78546:CDS |
| CCATCCCTTGAAACACTAGT+TGG | 0.514892 | 5.1:+60441155 | None:intergenic |
| CACATACAACACCATGCTTG+TGG | 0.523641 | 5.1:+60441201 | None:intergenic |
| TGAAAGCAGCTGCATGGGCA+TGG | 0.526257 | 5.1:-60441541 | MS.gene78546:CDS |
| GGTGTTGTATGTGGAAGATT+GGG | 0.531037 | 5.1:-60441191 | MS.gene78546:CDS |
| CCATAGTGAAAGCAGCTGCA+TGG | 0.531545 | 5.1:-60441547 | MS.gene78546:CDS |
| AAAGGAAGAAATTCACAAGA+AGG | 0.549669 | 5.1:-60441387 | MS.gene78546:CDS |
| TATTTATAACCCTTAGCAGA+AGG | 0.550417 | 5.1:+60441107 | None:intergenic |
| ACTTGTACTAGCAATATTGT+CGG | 0.557932 | 5.1:+60441277 | None:intergenic |
| TGATCCAACTAGTGTTTCAA+GGG | 0.561490 | 5.1:-60441159 | MS.gene78546:CDS |
| AAGAGTGTACCTTCTGCTAA+GGG | 0.573265 | 5.1:-60441116 | MS.gene78546:CDS |
| CCAACTAGTGTTTCAAGGGA+TGG | 0.579341 | 5.1:-60441155 | MS.gene78546:CDS |
| GCTTCTAATTTGTAACGAGA+AGG | 0.587034 | 5.1:+60441425 | None:intergenic |
| ATGTGGAAGATTGGGAGATG+TGG | 0.611419 | 5.1:-60441183 | MS.gene78546:CDS |
| GGTTCTGGATCATGTCATGA+AGG | 0.621349 | 5.1:-60441509 | MS.gene78546:CDS |
| CAATATTGCTAGTACAAGTG+TGG | 0.636698 | 5.1:-60441273 | MS.gene78546:CDS |
| TATTGCTAGTACAAGTGTGG+AGG | 0.666525 | 5.1:-60441270 | MS.gene78546:CDS |
| GCTAGTACAAGTGTGGAGGA+TGG | 0.684973 | 5.1:-60441266 | MS.gene78546:CDS |
| GCATGGGCATGGCATCAACG+TGG | 0.746007 | 5.1:-60441530 | MS.gene78546:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAGAAGAAGAAGATAAAGAA+AGG | - | chr5.1:60441514-60441533 | MS.gene78546:CDS | 25.0% |
| ! | AGAAGAAGAAGATAAAGAAA+GGG | - | chr5.1:60441515-60441534 | MS.gene78546:CDS | 25.0% |
| !!! | CAAGTAAGTTTTTTGTTACA+AGG | - | chr5.1:60441272-60441291 | MS.gene78546:CDS | 25.0% |
| AAAGGAAGAAATTCACAAGA+AGG | - | chr5.1:60441357-60441376 | MS.gene78546:CDS | 30.0% | |
| ACTTGTACTAGCAATATTGT+CGG | + | chr5.1:60441470-60441489 | None:intergenic | 30.0% | |
| ATTAGAAGCAGTGAAATCAA+TGG | - | chr5.1:60441333-60441352 | MS.gene78546:CDS | 30.0% | |
| TATTTATAACCCTTAGCAGA+AGG | + | chr5.1:60441640-60441659 | None:intergenic | 30.0% | |
| ! | ATGAAGTTCAAAGCATTTCA+AGG | - | chr5.1:60441401-60441420 | MS.gene78546:CDS | 30.0% |
| AGAGTTTAGAAATGAGAGCT+TGG | + | chr5.1:60441116-60441135 | None:intergenic | 35.0% | |
| AGCAGTGAAATCAATGGAAA+AGG | - | chr5.1:60441339-60441358 | MS.gene78546:CDS | 35.0% | |
| CAATATTGCTAGTACAAGTG+TGG | - | chr5.1:60441471-60441490 | MS.gene78546:CDS | 35.0% | |
| TGATCCAACTAGTGTTTCAA+GGG | - | chr5.1:60441585-60441604 | MS.gene78546:CDS | 35.0% | |
| TTGATCCAACTAGTGTTTCA+AGG | - | chr5.1:60441584-60441603 | MS.gene78546:CDS | 35.0% | |
| ! | GCTTCTAATTTGTAACGAGA+AGG | + | chr5.1:60441322-60441341 | None:intergenic | 35.0% |
| TATTGCTAGTACAAGTGTGG+AGG | - | chr5.1:60441474-60441493 | MS.gene78546:CDS | 40.0% | |
| ! | AAAGGGTTTTTCCACAAGCA+TGG | - | chr5.1:60441532-60441551 | MS.gene78546:CDS | 40.0% |
| ! | AAGAGTGTACCTTCTGCTAA+GGG | - | chr5.1:60441628-60441647 | MS.gene78546:CDS | 40.0% |
| ! | GGTGTTGTATGTGGAAGATT+GGG | - | chr5.1:60441553-60441572 | MS.gene78546:CDS | 40.0% |
| ! | TAAGAGTGTACCTTCTGCTA+AGG | - | chr5.1:60441627-60441646 | MS.gene78546:CDS | 40.0% |
| !! | TGGTGTTGTATGTGGAAGAT+TGG | - | chr5.1:60441552-60441571 | MS.gene78546:CDS | 40.0% |
| ATGTGGAAGATTGGGAGATG+TGG | - | chr5.1:60441561-60441580 | MS.gene78546:CDS | 45.0% | |
| CACATACAACACCATGCTTG+TGG | + | chr5.1:60441546-60441565 | None:intergenic | 45.0% | |
| CATAGTGAAAGCAGCTGCAT+GGG | - | chr5.1:60441198-60441217 | MS.gene78546:CDS | 45.0% | |
| CCAACTAGTGTTTCAAGGGA+TGG | - | chr5.1:60441589-60441608 | MS.gene78546:CDS | 45.0% | |
| CCATCCCTTGAAACACTAGT+TGG | + | chr5.1:60441592-60441611 | None:intergenic | 45.0% | |
| ! | GTGTGGAGGATGGTGTTAAA+AGG | - | chr5.1:60441488-60441507 | MS.gene78546:CDS | 45.0% |
| !! | CACAAGCATGGTGTTGTATG+TGG | - | chr5.1:60441544-60441563 | MS.gene78546:CDS | 45.0% |
| !! | GGTTCTGGATCATGTCATGA+AGG | - | chr5.1:60441235-60441254 | MS.gene78546:CDS | 45.0% |
| CCATAGTGAAAGCAGCTGCA+TGG | - | chr5.1:60441197-60441216 | MS.gene78546:CDS | 50.0% | |
| GCTAGTACAAGTGTGGAGGA+TGG | - | chr5.1:60441478-60441497 | MS.gene78546:CDS | 50.0% | |
| ! | CCATGCAGCTGCTTTCACTA+TGG | + | chr5.1:60441200-60441219 | None:intergenic | 50.0% |
| GCATGGCATCAACGTGGTTC+TGG | - | chr5.1:60441220-60441239 | MS.gene78546:CDS | 55.0% | |
| TGAAAGCAGCTGCATGGGCA+TGG | - | chr5.1:60441203-60441222 | MS.gene78546:CDS | 55.0% | |
| GCATGGGCATGGCATCAACG+TGG | - | chr5.1:60441214-60441233 | MS.gene78546:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 60441106 | 60441660 | 60441106 | ID=MS.gene78546 |
| chr5.1 | mRNA | 60441106 | 60441660 | 60441106 | ID=MS.gene78546.t1;Parent=MS.gene78546 |
| chr5.1 | exon | 60441106 | 60441660 | 60441106 | ID=MS.gene78546.t1.exon1;Parent=MS.gene78546.t1 |
| chr5.1 | CDS | 60441106 | 60441660 | 60441106 | ID=cds.MS.gene78546.t1;Parent=MS.gene78546.t1 |
| Gene Sequence |
| Protein sequence |
