Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene78986.t1 | XP_003628241.1 | 98.6 | 146 | 2 | 0 | 12 | 157 | 173 | 318 | 5.60E-79 | 303.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene78986.t1 | G7L8J3 | 98.6 | 146 | 2 | 0 | 12 | 157 | 173 | 318 | 4.0e-79 | 303.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050226 | MS.gene78986 | 0.80072 | 1.26E-48 | -1.69E-46 |
| MS.gene050227 | MS.gene78986 | 0.803099 | 4.10E-49 | -1.69E-46 |
| MS.gene050228 | MS.gene78986 | 0.805863 | 1.09E-49 | -1.69E-46 |
| MS.gene050438 | MS.gene78986 | 0.810905 | 9.13E-51 | -1.69E-46 |
| MS.gene050558 | MS.gene78986 | 0.814159 | 1.78E-51 | -1.69E-46 |
| MS.gene050719 | MS.gene78986 | 0.826786 | 2.24E-54 | -1.69E-46 |
| MS.gene050721 | MS.gene78986 | 0.827875 | 1.23E-54 | -1.69E-46 |
| MS.gene050724 | MS.gene78986 | 0.827762 | 1.31E-54 | -1.69E-46 |
| MS.gene052176 | MS.gene78986 | 0.802654 | 5.06E-49 | -1.69E-46 |
| MS.gene053836 | MS.gene78986 | 0.820073 | 8.32E-53 | -1.69E-46 |
| MS.gene058906 | MS.gene78986 | 0.806507 | 7.96E-50 | -1.69E-46 |
| MS.gene059124 | MS.gene78986 | 0.815627 | 8.39E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene78986.t1 | MTR_8g046180 | 98.630 | 146 | 2 | 0 | 12 | 157 | 173 | 318 | 2.36e-104 | 301 |
| MS.gene78986.t1 | MTR_8g046180 | 96.491 | 114 | 4 | 0 | 12 | 125 | 173 | 286 | 9.01e-78 | 233 |
| MS.gene78986.t1 | MTR_6g004030 | 45.139 | 144 | 79 | 0 | 14 | 157 | 158 | 301 | 9.41e-45 | 149 |
| MS.gene78986.t1 | MTR_8g046180 | 93.878 | 49 | 3 | 0 | 11 | 59 | 172 | 220 | 1.66e-28 | 105 |
| MS.gene78986.t1 | MTR_6g004030 | 45.977 | 87 | 47 | 0 | 14 | 100 | 158 | 244 | 6.17e-24 | 94.0 |
| MS.gene78986.t1 | MTR_8g446720 | 74.000 | 50 | 12 | 1 | 108 | 157 | 8 | 56 | 1.73e-17 | 72.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene78986.t1 | AT4G29400 | 76.027 | 146 | 35 | 0 | 12 | 157 | 157 | 302 | 3.87e-82 | 244 |
| MS.gene78986.t1 | AT5G08400 | 45.833 | 144 | 78 | 0 | 14 | 157 | 157 | 300 | 6.89e-45 | 149 |
| MS.gene78986.t1 | AT5G08400 | 45.833 | 144 | 78 | 0 | 14 | 157 | 190 | 333 | 1.29e-44 | 149 |
Find 24 sgRNAs with CRISPR-Local
Find 53 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACAACTTACTTGCATGTTCA+TGG | 0.294111 | 8.3:+53425476 | None:intergenic |
| ACCAAGTTACGATTATATTC+AGG | 0.316714 | 8.3:-53425312 | MS.gene78986:CDS |
| GATTTAACATCAGAGGATTC+AGG | 0.317872 | 8.3:-53424858 | MS.gene78986:CDS |
| ACATCAGAGGATTCAGGTTA+TGG | 0.330697 | 8.3:-53424852 | MS.gene78986:CDS |
| TGTCTTTCTTACCATGATAC+TGG | 0.337063 | 8.3:-53425730 | MS.gene78986:CDS |
| GTGGAGAAGAATTTGAGAAT+TGG | 0.339234 | 8.3:-53424887 | MS.gene78986:CDS |
| TGATATAACAGGATAGATAT+TGG | 0.346310 | 8.3:-53425121 | MS.gene78986:intron |
| AAGACAACTTGGCTCGAATA+TGG | 0.374877 | 8.3:-53425238 | MS.gene78986:intron |
| ATCAAAACATCTAGAATCAA+TGG | 0.409600 | 8.3:+53425088 | None:intergenic |
| AGAATTTGAGAATTGGAAAG+AGG | 0.431631 | 8.3:-53424880 | MS.gene78986:CDS |
| CCCTGAATATAATCGTAACT+TGG | 0.440268 | 8.3:+53425311 | None:intergenic |
| GATATAACAGGATAGATATT+GGG | 0.451457 | 8.3:-53425120 | MS.gene78986:intron |
| CCAAGTTACGATTATATTCA+GGG | 0.463616 | 8.3:-53425311 | MS.gene78986:CDS |
| ATTCGAGCATGGCACATTGT+AGG | 0.492114 | 8.3:-53425519 | MS.gene78986:CDS |
| ATCTGGAAATGCCAGTATCA+TGG | 0.497218 | 8.3:+53425719 | None:intergenic |
| GGGATCAAGCAAGTAGTGTT+TGG | 0.510754 | 8.3:-53424909 | MS.gene78986:CDS |
| TCTTGAGGTTCAAGACAACT+TGG | 0.535517 | 8.3:-53425249 | MS.gene78986:CDS |
| TTACAACATTGGGGATCTTG+AGG | 0.541473 | 8.3:-53425264 | MS.gene78986:CDS |
| CCAATGTTGTAAAATGTAGT+TGG | 0.552417 | 8.3:+53425275 | None:intergenic |
| ATCAAGCAAGTAGTGTTTGG+TGG | 0.565132 | 8.3:-53424906 | MS.gene78986:CDS |
| GAAAGAGGATTTAACATCAG+AGG | 0.565258 | 8.3:-53424865 | MS.gene78986:CDS |
| CTTTGCAGGCTATTCGAGCA+TGG | 0.569213 | 8.3:-53425530 | MS.gene78986:intron |
| TGGCACATTGTAGGACGACT+TGG | 0.571804 | 8.3:-53425510 | MS.gene78986:CDS |
| CACATTGTAGGACGACTTGG+AGG | 0.594434 | 8.3:-53425507 | MS.gene78986:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAATTCACTATAAATAGAA+AGG | + | chr8.3:53425169-53425188 | None:intergenic | 15.0% |
| !!! | ATTTATTTTTGTGATATAAC+AGG | - | chr8.3:53425440-53425459 | MS.gene78986:intron | 15.0% |
| !!! | ATTTTTCAAACTCATATATA+TGG | - | chr8.3:53424879-53424898 | MS.gene78986:CDS | 15.0% |
| !! | AAAAAACCAGATCAATTAAT+TGG | + | chr8.3:53425624-53425643 | None:intergenic | 20.0% |
| !! | AATTATATTGCTTATAGAAC+TGG | - | chr8.3:53425197-53425216 | MS.gene78986:intron | 20.0% |
| !! | ATTAATCCAATTAATTGATC+TGG | - | chr8.3:53425615-53425634 | MS.gene78986:intron | 20.0% |
| !! | GTAAAACAAAATAAAGTCAT+AGG | + | chr8.3:53424970-53424989 | None:intergenic | 20.0% |
| ! | AACAAAATAAAGTCATAGGA+TGG | + | chr8.3:53424966-53424985 | None:intergenic | 25.0% |
| ! | AATGAGAGAGAAAGAAAATT+TGG | + | chr8.3:53425143-53425162 | None:intergenic | 25.0% |
| ! | GATATAACAGGATAGATATT+GGG | - | chr8.3:53425452-53425471 | MS.gene78986:intron | 25.0% |
| ! | GTAGTTAATTAAACAAACAG+AGG | + | chr8.3:53425552-53425571 | None:intergenic | 25.0% |
| ! | TGAGTTTGAAAAATCAAATC+TGG | + | chr8.3:53424873-53424892 | None:intergenic | 25.0% |
| ! | TGATATAACAGGATAGATAT+TGG | - | chr8.3:53425451-53425470 | MS.gene78986:intron | 25.0% |
| !! | AACTACATTTTACAACATTG+GGG | - | chr8.3:53425299-53425318 | MS.gene78986:CDS | 25.0% |
| !! | ATCAAAACATCTAGAATCAA+TGG | + | chr8.3:53425487-53425506 | None:intergenic | 25.0% |
| !! | CAACTACATTTTACAACATT+GGG | - | chr8.3:53425298-53425317 | MS.gene78986:CDS | 25.0% |
| !! | TAACAAAGCATTAAGAATGA+AGG | + | chr8.3:53425380-53425399 | None:intergenic | 25.0% |
| !!! | TGTTTTTTGATAGCTTTGTT+GGG | - | chr8.3:53425643-53425662 | MS.gene78986:CDS | 25.0% |
| !!! | TTGTTTTTTGATAGCTTTGT+TGG | - | chr8.3:53425642-53425661 | MS.gene78986:CDS | 25.0% |
| AAACATGTAATGAGAGAGAA+AGG | + | chr8.3:53425593-53425612 | None:intergenic | 30.0% | |
| AACATGTAATGAGAGAGAAA+GGG | + | chr8.3:53425592-53425611 | None:intergenic | 30.0% | |
| AATTAAACAAACAGAGGAAG+TGG | + | chr8.3:53425546-53425565 | None:intergenic | 30.0% | |
| ACCAAGTTACGATTATATTC+AGG | - | chr8.3:53425260-53425279 | MS.gene78986:CDS | 30.0% | |
| AGAATTTGAGAATTGGAAAG+AGG | - | chr8.3:53425692-53425711 | MS.gene78986:CDS | 30.0% | |
| CCAAGTTACGATTATATTCA+GGG | - | chr8.3:53425261-53425280 | MS.gene78986:CDS | 30.0% | |
| CCAATGTTGTAAAATGTAGT+TGG | + | chr8.3:53425300-53425319 | None:intergenic | 30.0% | |
| ! | CCAACTACATTTTACAACAT+TGG | - | chr8.3:53425297-53425316 | MS.gene78986:CDS | 30.0% |
| ! | GAAAGAAAATTTGGTTTCCA+TGG | + | chr8.3:53425134-53425153 | None:intergenic | 30.0% |
| ! | TGAATTTTACAATGCTCCAT+TGG | - | chr8.3:53424904-53424923 | MS.gene78986:CDS | 30.0% |
| ! | TTAGGAAGTTTTGCTGAATT+TGG | - | chr8.3:53425017-53425036 | MS.gene78986:intron | 30.0% |
| ACAACTTACTTGCATGTTCA+TGG | + | chr8.3:53425099-53425118 | None:intergenic | 35.0% | |
| CCCTGAATATAATCGTAACT+TGG | + | chr8.3:53425264-53425283 | None:intergenic | 35.0% | |
| GAAAGAGGATTTAACATCAG+AGG | - | chr8.3:53425707-53425726 | MS.gene78986:CDS | 35.0% | |
| GATTTAACATCAGAGGATTC+AGG | - | chr8.3:53425714-53425733 | MS.gene78986:CDS | 35.0% | |
| GTGGAGAAGAATTTGAGAAT+TGG | - | chr8.3:53425685-53425704 | MS.gene78986:CDS | 35.0% | |
| TGTCTTTCTTACCATGATAC+TGG | - | chr8.3:53424842-53424861 | MS.gene78986:CDS | 35.0% | |
| !!! | AAGTTGATGTCTTTTGCCAA+TGG | + | chr8.3:53424923-53424942 | None:intergenic | 35.0% |
| ATCAAGCAAGTAGTGTTTGG+TGG | - | chr8.3:53425666-53425685 | MS.gene78986:CDS | 40.0% | |
| ATCTGGAAATGCCAGTATCA+TGG | + | chr8.3:53424856-53424875 | None:intergenic | 40.0% | |
| GATGCACACATGCTTCAATT+AGG | - | chr8.3:53424999-53425018 | MS.gene78986:intron | 40.0% | |
| TCTTGAGGTTCAAGACAACT+TGG | - | chr8.3:53425323-53425342 | MS.gene78986:CDS | 40.0% | |
| TGCTGAATTTGGATCTTTGC+AGG | - | chr8.3:53425028-53425047 | MS.gene78986:intron | 40.0% | |
| TTACAACATTGGGGATCTTG+AGG | - | chr8.3:53425308-53425327 | MS.gene78986:CDS | 40.0% | |
| ! | AAGACAACTTGGCTCGAATA+TGG | - | chr8.3:53425334-53425353 | MS.gene78986:CDS | 40.0% |
| ! | ACATCAGAGGATTCAGGTTA+TGG | - | chr8.3:53425720-53425739 | MS.gene78986:CDS | 40.0% |
| !!! | GTTGTTTTTCTCTTCTGCCA+TGG | - | chr8.3:53425114-53425133 | MS.gene78986:intron | 40.0% |
| ATTCGAGCATGGCACATTGT+AGG | - | chr8.3:53425053-53425072 | MS.gene78986:intron | 45.0% | |
| ATTGAAGCATGTGTGCATCG+TGG | + | chr8.3:53424998-53425017 | None:intergenic | 45.0% | |
| GGGATCAAGCAAGTAGTGTT+TGG | - | chr8.3:53425663-53425682 | MS.gene78986:CDS | 45.0% | |
| CTTTGCAGGCTATTCGAGCA+TGG | - | chr8.3:53425042-53425061 | MS.gene78986:intron | 50.0% | |
| GAGGAAGTGGAGATCGTACT+CGG | + | chr8.3:53425533-53425552 | None:intergenic | 50.0% | |
| TGGCACATTGTAGGACGACT+TGG | - | chr8.3:53425062-53425081 | MS.gene78986:CDS | 50.0% | |
| ! | CACATTGTAGGACGACTTGG+AGG | - | chr8.3:53425065-53425084 | MS.gene78986:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 53424836 | 53425758 | 53424836 | ID=MS.gene78986 |
| chr8.3 | mRNA | 53424836 | 53425758 | 53424836 | ID=MS.gene78986.t1;Parent=MS.gene78986 |
| chr8.3 | exon | 53425642 | 53425758 | 53425642 | ID=MS.gene78986.t1.exon1;Parent=MS.gene78986.t1 |
| chr8.3 | CDS | 53425642 | 53425758 | 53425642 | ID=cds.MS.gene78986.t1;Parent=MS.gene78986.t1 |
| chr8.3 | exon | 53425485 | 53425544 | 53425485 | ID=MS.gene78986.t1.exon2;Parent=MS.gene78986.t1 |
| chr8.3 | CDS | 53425485 | 53425544 | 53425485 | ID=cds.MS.gene78986.t1;Parent=MS.gene78986.t1 |
| chr8.3 | exon | 53425239 | 53425360 | 53425239 | ID=MS.gene78986.t1.exon3;Parent=MS.gene78986.t1 |
| chr8.3 | CDS | 53425239 | 53425360 | 53425239 | ID=cds.MS.gene78986.t1;Parent=MS.gene78986.t1 |
| chr8.3 | exon | 53425061 | 53425132 | 53425061 | ID=MS.gene78986.t1.exon4;Parent=MS.gene78986.t1 |
| chr8.3 | CDS | 53425061 | 53425132 | 53425061 | ID=cds.MS.gene78986.t1;Parent=MS.gene78986.t1 |
| chr8.3 | exon | 53424836 | 53424938 | 53424836 | ID=MS.gene78986.t1.exon5;Parent=MS.gene78986.t1 |
| chr8.3 | CDS | 53424836 | 53424938 | 53424836 | ID=cds.MS.gene78986.t1;Parent=MS.gene78986.t1 |
| Gene Sequence |
| Protein sequence |
