Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79257.t1 | RHN75196.1 | 91.8 | 61 | 5 | 0 | 9 | 69 | 498 | 558 | 5.40E-19 | 104 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79257.t1 | A0A072VBM0 | 91.8 | 61 | 5 | 0 | 9 | 69 | 498 | 558 | 3.9e-19 | 104.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050317 | MS.gene79257 | 0.810602 | 1.06E-50 | -1.69E-46 |
| MS.gene051343 | MS.gene79257 | 0.809991 | 1.44E-50 | -1.69E-46 |
| MS.gene051676 | MS.gene79257 | 0.824901 | 6.28E-54 | -1.69E-46 |
| MS.gene051902 | MS.gene79257 | 0.803644 | 3.16E-49 | -1.69E-46 |
| MS.gene052434 | MS.gene79257 | 0.803785 | 2.95E-49 | -1.69E-46 |
| MS.gene057890 | MS.gene79257 | -0.817426 | 3.32E-52 | -1.69E-46 |
| MS.gene058638 | MS.gene79257 | 0.820212 | 7.73E-53 | -1.69E-46 |
| MS.gene058639 | MS.gene79257 | 0.833049 | 6.67E-56 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79257.t1 | MTR_2g081100 | 90.909 | 66 | 6 | 0 | 9 | 74 | 498 | 563 | 9.18e-32 | 118 |
| MS.gene79257.t1 | MTR_2g081100 | 90.909 | 66 | 6 | 0 | 9 | 74 | 498 | 563 | 1.08e-31 | 118 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 35 sgRNAs with CRISPR-Local
Find 76 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTAAGGCTTGGTTTCATTA+AGG | 0.279842 | 1.4:-57211884 | MS.gene79257:intron |
| CTGAGGATACTGTGTTCTTC+CGG | 0.331177 | 1.4:-57210861 | MS.gene79257:CDS |
| AAGTCTCTGGTATGTAAAAT+TGG | 0.342123 | 1.4:-57211054 | MS.gene79257:CDS |
| AAAGCCAACAAATGACATTA+AGG | 0.345467 | 1.4:-57211900 | None:intergenic |
| TGAGGATACTGTGTTCTTCC+GGG | 0.383874 | 1.4:-57210860 | MS.gene79257:CDS |
| TCTTGGTGCTCAGTCCTTTG+AGG | 0.398508 | 1.4:-57211723 | MS.gene79257:intron |
| TCTGTGAATAACCATATGAT+TGG | 0.408436 | 1.4:-57210889 | MS.gene79257:CDS |
| AGTGAATTGTGAAGATTCCT+TGG | 0.446636 | 1.4:+57211745 | None:intergenic |
| CCTCAGCTTGCCCAATCATA+TGG | 0.450278 | 1.4:+57210878 | None:intergenic |
| CCTTTCCGACCTCTAGTGCT+TGG | 0.456216 | 1.4:+57211483 | None:intergenic |
| TCATCATCGCTGAAGGAAAC+CGG | 0.458878 | 1.4:+57211534 | None:intergenic |
| GAATCTTCACAATTCACTCT+TGG | 0.470201 | 1.4:-57211740 | MS.gene79257:CDS |
| TGCAGCTGGAGCTGATAGTC+CGG | 0.486818 | 1.4:-57211553 | MS.gene79257:CDS |
| CTGTGAATAACCATATGATT+GGG | 0.489653 | 1.4:-57210888 | MS.gene79257:CDS |
| TTTGTTCTAGGAACGTGTTA+AGG | 0.492658 | 1.4:-57211783 | MS.gene79257:intron |
| AGAGCAAGACAAAACCTTCC+CGG | 0.495380 | 1.4:+57210842 | None:intergenic |
| CAACAAATGACATTAAGGCT+TGG | 0.502037 | 1.4:-57211895 | None:intergenic |
| TTTCAAGGCAGAAGTGCAGC+TGG | 0.505124 | 1.4:-57211567 | MS.gene79257:CDS |
| CGGTTTGCACCAGAACTTGG+TGG | 0.505268 | 1.4:-57210746 | MS.gene79257:intron |
| ATTTGTTGGGTTTACCTCAA+AGG | 0.513130 | 1.4:+57211709 | None:intergenic |
| TGTAAAATTGGAGGTGAGTG+GGG | 0.520147 | 1.4:-57211042 | MS.gene79257:CDS |
| TGTGCAAGTACACAAGTCTC+TGG | 0.520349 | 1.4:-57211067 | MS.gene79257:CDS |
| TTTATGTCTTAAGCTAGTAA+AGG | 0.520978 | 1.4:+57210699 | None:intergenic |
| ATGTAAAATTGGAGGTGAGT+GGG | 0.523440 | 1.4:-57211043 | MS.gene79257:CDS |
| GATACTGTGTTCTTCCGGGA+AGG | 0.549324 | 1.4:-57210856 | MS.gene79257:intron |
| GGAGCGATCTACGCGCTCCA+AGG | 0.554334 | 1.4:-57211762 | MS.gene79257:CDS |
| GGCATCTTCATCATCGCTGA+AGG | 0.569407 | 1.4:+57211527 | None:intergenic |
| TCTCTGGTATGTAAAATTGG+AGG | 0.581652 | 1.4:-57211051 | MS.gene79257:CDS |
| AGCTCAAAACCAAGCACTAG+AGG | 0.589923 | 1.4:-57211492 | MS.gene79257:CDS |
| CCAAGCACTAGAGGTCGGAA+AGG | 0.605138 | 1.4:-57211483 | MS.gene79257:CDS |
| ACTTGGTGGTGATATTGACT+TGG | 0.615160 | 1.4:-57210732 | MS.gene79257:CDS |
| GAGTGCTTTGCAAAGCACGC+AGG | 0.616754 | 1.4:-57211020 | MS.gene79257:intron |
| TATGTAAAATTGGAGGTGAG+TGG | 0.623493 | 1.4:-57211044 | MS.gene79257:CDS |
| CCATATGATTGGGCAAGCTG+AGG | 0.633601 | 1.4:-57210878 | MS.gene79257:CDS |
| CAAAACCAAGCACTAGAGGT+CGG | 0.637760 | 1.4:-57211488 | MS.gene79257:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | GTCAATTTTTTATTATTTTT+TGG | - | chr1.4:57211789-57211808 | MS.gene79257:intron | 10.0% |
| !! | AATGTATAATATCATTTGTT+GGG | + | chr1.4:57210908-57210927 | None:intergenic | 15.0% |
| !! | TTGAAAGAAAGAAAAAAAAA+AGG | + | chr1.4:57211247-57211266 | None:intergenic | 15.0% |
| !! | CAATGTATAATATCATTTGT+TGG | + | chr1.4:57210909-57210928 | None:intergenic | 20.0% |
| !! | TGAAAATTATGTAAAAAGCA+GGG | - | chr1.4:57210947-57210966 | MS.gene79257:intron | 20.0% |
| !! | TTGAAAATTATGTAAAAAGC+AGG | - | chr1.4:57210946-57210965 | MS.gene79257:intron | 20.0% |
| !!! | TTGTGATCAGAATTATTTTA+TGG | - | chr1.4:57210757-57210776 | MS.gene79257:intron | 20.0% |
| ! | AAAGAAAAAAAAAAGGTGAG+AGG | + | chr1.4:57211240-57211259 | None:intergenic | 25.0% |
| ! | AAGAAATAAGCTAAGAATGA+AGG | + | chr1.4:57210999-57211018 | None:intergenic | 25.0% |
| ! | ATCATCATCATAACTGTTAA+TGG | - | chr1.4:57211384-57211403 | MS.gene79257:intron | 25.0% |
| !! | ATCACTTTCATTGATTATTG+TGG | - | chr1.4:57211484-57211503 | MS.gene79257:CDS | 25.0% |
| !! | TTATTTCTTTATTCCGTACA+GGG | - | chr1.4:57211010-57211029 | MS.gene79257:intron | 25.0% |
| !!! | ATCTATCTATGATTTGTTCA+TGG | - | chr1.4:57211661-57211680 | MS.gene79257:intron | 25.0% |
| !!! | ATTTTTTGGTTCTGATATGT+TGG | - | chr1.4:57211803-57211822 | MS.gene79257:intron | 25.0% |
| AAGTCTCTGGTATGTAAAAT+TGG | - | chr1.4:57211547-57211566 | MS.gene79257:CDS | 30.0% | |
| ATAATAGTATGATCAGTGAC+TGG | - | chr1.4:57210782-57210801 | MS.gene79257:intron | 30.0% | |
| CTGTGAATAACCATATGATT+GGG | - | chr1.4:57211713-57211732 | MS.gene79257:intron | 30.0% | |
| TCTGTGAATAACCATATGAT+TGG | - | chr1.4:57211712-57211731 | MS.gene79257:intron | 30.0% | |
| ! | ATCGTTCTTCTTTTTGTTCT+AGG | - | chr1.4:57210806-57210825 | MS.gene79257:intron | 30.0% |
| ! | ATGACACTAATATCCTTTTC+CGG | - | chr1.4:57211835-57211854 | MS.gene79257:intron | 30.0% |
| ! | ATTAAGGCTTGGTTTCATTA+AGG | - | chr1.4:57210717-57210736 | MS.gene79257:CDS | 30.0% |
| ! | CTTATTTCTTTATTCCGTAC+AGG | - | chr1.4:57211009-57211028 | MS.gene79257:intron | 30.0% |
| ! | TATGTAAAAAGCAGGGTTTT+GGG | - | chr1.4:57210954-57210973 | MS.gene79257:intron | 30.0% |
| ! | TTATGTAAAAAGCAGGGTTT+TGG | - | chr1.4:57210953-57210972 | MS.gene79257:intron | 30.0% |
| !! | TCTAATTCTTGCTGTTACTA+AGG | - | chr1.4:57211621-57211640 | MS.gene79257:intron | 30.0% |
| AGAAGCTAACAAATCTGCAA+AGG | + | chr1.4:57211207-57211226 | None:intergenic | 35.0% | |
| AGAGAAGACTAATATCGTGA+TGG | + | chr1.4:57211168-57211187 | None:intergenic | 35.0% | |
| AGTGAATTGTGAAGATTCCT+TGG | + | chr1.4:57210859-57210878 | None:intergenic | 35.0% | |
| ATGTAAAATTGGAGGTGAGT+GGG | - | chr1.4:57211558-57211577 | MS.gene79257:CDS | 35.0% | |
| ATTTATGAGCGTTATGAGAC+TGG | - | chr1.4:57211297-57211316 | MS.gene79257:intron | 35.0% | |
| GAATCTTCACAATTCACTCT+TGG | - | chr1.4:57210861-57210880 | MS.gene79257:CDS | 35.0% | |
| TATGTAAAATTGGAGGTGAG+TGG | - | chr1.4:57211557-57211576 | MS.gene79257:CDS | 35.0% | |
| TCTCTGGTATGTAAAATTGG+AGG | - | chr1.4:57211550-57211569 | MS.gene79257:CDS | 35.0% | |
| ! | ATTTGTTGGGTTTACCTCAA+AGG | + | chr1.4:57210895-57210914 | None:intergenic | 35.0% |
| !! | CACGATATTAGTCTTCTCTT+TGG | - | chr1.4:57211169-57211188 | MS.gene79257:intron | 35.0% |
| !! | TTTGTTCTAGGAACGTGTTA+AGG | - | chr1.4:57210818-57210837 | MS.gene79257:intron | 35.0% |
| AGACTGGTGTACATCTATGA+TGG | - | chr1.4:57211313-57211332 | MS.gene79257:intron | 40.0% | |
| TCAATATCACCACCAAGTTC+TGG | + | chr1.4:57211867-57211886 | None:intergenic | 40.0% | |
| TGTAAAATTGGAGGTGAGTG+GGG | - | chr1.4:57211559-57211578 | MS.gene79257:CDS | 40.0% | |
| ! | TATTCCGTACAGGGTTTTCA+AGG | - | chr1.4:57211019-57211038 | MS.gene79257:intron | 40.0% |
| ! | TTTTGAGCTTGATGTTGGCA+TGG | + | chr1.4:57211098-57211117 | None:intergenic | 40.0% |
| !! | ACTTGGTGGTGATATTGACT+TGG | - | chr1.4:57211869-57211888 | MS.gene79257:intron | 40.0% |
| !! | TCTTGCTGTTACTAAGGAGA+GGG | - | chr1.4:57211627-57211646 | MS.gene79257:intron | 40.0% |
| !! | TTCTTGCTGTTACTAAGGAG+AGG | - | chr1.4:57211626-57211645 | MS.gene79257:intron | 40.0% |
| !!! | CTTGGTTTTGAGCTTGATGT+TGG | + | chr1.4:57211103-57211122 | None:intergenic | 40.0% |
| AGAGCAAGACAAAACCTTCC+CGG | + | chr1.4:57211762-57211781 | None:intergenic | 45.0% | |
| AGATACTCTCCATCATCGCA+TGG | + | chr1.4:57211688-57211707 | None:intergenic | 45.0% | |
| AGCTCAAAACCAAGCACTAG+AGG | - | chr1.4:57211109-57211128 | MS.gene79257:intron | 45.0% | |
| CAAAACCAAGCACTAGAGGT+CGG | - | chr1.4:57211113-57211132 | MS.gene79257:intron | 45.0% | |
| CTGAGGATACTGTGTTCTTC+CGG | - | chr1.4:57211740-57211759 | MS.gene79257:CDS | 45.0% | |
| TCATCATCGCTGAAGGAAAC+CGG | + | chr1.4:57211070-57211089 | None:intergenic | 45.0% | |
| TCTGCCTTGAAAACCCTGTA+CGG | + | chr1.4:57211026-57211045 | None:intergenic | 45.0% | |
| TGAGGATACTGTGTTCTTCC+GGG | - | chr1.4:57211741-57211760 | MS.gene79257:CDS | 45.0% | |
| TGTGCAAGTACACAAGTCTC+TGG | - | chr1.4:57211534-57211553 | MS.gene79257:CDS | 45.0% | |
| ! | TTTTCACAGAAGCCTGACCA+TGG | + | chr1.4:57211439-57211458 | None:intergenic | 45.0% |
| !!! | TAAAAAGCAGGGTTTTGGGC+AGG | - | chr1.4:57210958-57210977 | MS.gene79257:intron | 45.0% |
| AAGCACGCAGGTTTGTCGAA+TGG | - | chr1.4:57211593-57211612 | MS.gene79257:intron | 50.0% | |
| CCATATGATTGGGCAAGCTG+AGG | - | chr1.4:57211723-57211742 | MS.gene79257:intron | 50.0% | |
| CCTCAGCTTGCCCAATCATA+TGG | + | chr1.4:57211726-57211745 | None:intergenic | 50.0% | |
| GATACTGTGTTCTTCCGGGA+AGG | - | chr1.4:57211745-57211764 | MS.gene79257:CDS | 50.0% | |
| GGCATCTTCATCATCGCTGA+AGG | + | chr1.4:57211077-57211096 | None:intergenic | 50.0% | |
| GTTCATGGACCATGCGATGA+TGG | - | chr1.4:57211676-57211695 | MS.gene79257:intron | 50.0% | |
| GTTCTGGTGCAAACCGGAAA+AGG | + | chr1.4:57211851-57211870 | None:intergenic | 50.0% | |
| ! | CACCAAGTTCTGGTGCAAAC+CGG | + | chr1.4:57211857-57211876 | None:intergenic | 50.0% |
| ! | TTCCGGTTTGCACCAGAACT+TGG | - | chr1.4:57211852-57211871 | MS.gene79257:intron | 50.0% |
| ! | TTTCAAGGCAGAAGTGCAGC+TGG | - | chr1.4:57211034-57211053 | MS.gene79257:CDS | 50.0% |
| !! | TCTTGGTGCTCAGTCCTTTG+AGG | - | chr1.4:57210878-57210897 | MS.gene79257:CDS | 50.0% |
| CCAAGCACTAGAGGTCGGAA+AGG | - | chr1.4:57211118-57211137 | MS.gene79257:intron | 55.0% | |
| CCTTTCCGACCTCTAGTGCT+TGG | + | chr1.4:57211121-57211140 | None:intergenic | 55.0% | |
| CGGTTTGCACCAGAACTTGG+TGG | - | chr1.4:57211855-57211874 | MS.gene79257:intron | 55.0% | |
| GAGTGCTTTGCAAAGCACGC+AGG | - | chr1.4:57211581-57211600 | MS.gene79257:intron | 55.0% | |
| TGCAGCTGGAGCTGATAGTC+CGG | - | chr1.4:57211048-57211067 | MS.gene79257:CDS | 55.0% | |
| TTTCAACGCAGCTCCTGCCA+TGG | - | chr1.4:57211419-57211438 | MS.gene79257:intron | 55.0% | |
| CACAGAAGCCTGACCATGGC+AGG | + | chr1.4:57211435-57211454 | None:intergenic | 60.0% | |
| ACGCAGCTCCTGCCATGGTC+AGG | - | chr1.4:57211424-57211443 | MS.gene79257:intron | 65.0% | |
| GGAGCGATCTACGCGCTCCA+AGG | - | chr1.4:57210839-57210858 | MS.gene79257:intron | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 57210712 | 57211911 | 57210712 | ID=MS.gene79257 |
| chr1.4 | mRNA | 57210712 | 57211911 | 57210712 | ID=MS.gene79257.t1;Parent=MS.gene79257 |
| chr1.4 | exon | 57211885 | 57211911 | 57211885 | ID=MS.gene79257.t1.exon1;Parent=MS.gene79257.t1 |
| chr1.4 | CDS | 57211885 | 57211911 | 57211885 | ID=cds.MS.gene79257.t1;Parent=MS.gene79257.t1 |
| chr1.4 | exon | 57211724 | 57211795 | 57211724 | ID=MS.gene79257.t1.exon2;Parent=MS.gene79257.t1 |
| chr1.4 | CDS | 57211724 | 57211795 | 57211724 | ID=cds.MS.gene79257.t1;Parent=MS.gene79257.t1 |
| chr1.4 | exon | 57211466 | 57211592 | 57211466 | ID=MS.gene79257.t1.exon3;Parent=MS.gene79257.t1 |
| chr1.4 | CDS | 57211466 | 57211592 | 57211466 | ID=cds.MS.gene79257.t1;Parent=MS.gene79257.t1 |
| chr1.4 | exon | 57211021 | 57211090 | 57211021 | ID=MS.gene79257.t1.exon4;Parent=MS.gene79257.t1 |
| chr1.4 | CDS | 57211021 | 57211090 | 57211021 | ID=cds.MS.gene79257.t1;Parent=MS.gene79257.t1 |
| chr1.4 | exon | 57210857 | 57210912 | 57210857 | ID=MS.gene79257.t1.exon5;Parent=MS.gene79257.t1 |
| chr1.4 | CDS | 57210857 | 57210912 | 57210857 | ID=cds.MS.gene79257.t1;Parent=MS.gene79257.t1 |
| chr1.4 | exon | 57210712 | 57210755 | 57210712 | ID=MS.gene79257.t1.exon6;Parent=MS.gene79257.t1 |
| chr1.4 | CDS | 57210712 | 57210755 | 57210712 | ID=cds.MS.gene79257.t1;Parent=MS.gene79257.t1 |
| Gene Sequence |
| Protein sequence |
