AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene80300

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene80300.t1 MTR_6g059930 95.750 400 17 0 2 401 52 451 0.0 761
MS.gene80300.t1 MTR_6g059930 95.750 400 17 0 2 401 20 419 0.0 761
MS.gene80300.t1 MTR_2g088390 45.676 370 197 3 34 401 80 447 9.61e-118 350
MS.gene80300.t1 MTR_2g067610 47.531 324 167 2 79 401 8 329 1.26e-108 322
MS.gene80300.t1 MTR_5g072520 41.695 295 152 3 126 400 2 296 6.85e-81 255
MS.gene80300.t1 MTR_5g072520 30.159 126 87 1 93 217 161 286 1.84e-13 72.0
MS.gene80300.t1 MTR_2g088750 38.591 298 154 3 90 387 55 323 2.14e-68 220
MS.gene80300.t1 MTR_2g088750 27.500 120 87 0 261 380 54 173 1.81e-12 68.6
MS.gene80300.t1 MTR_1g054885 53.247 77 34 2 321 396 2 77 3.46e-19 82.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene80300.t1 AT1G64810 65.206 388 133 2 16 401 73 460 0.0 557
MS.gene80300.t1 AT1G64810 65.206 388 133 2 16 401 49 436 0.0 557
MS.gene80300.t1 AT5G57930 43.377 385 212 3 17 400 63 442 1.48e-110 332
MS.gene80300.t1 AT5G57930 43.377 385 212 3 17 400 60 439 2.03e-110 331
MS.gene80300.t1 AT5G61930 43.562 365 196 6 40 401 45 402 1.14e-102 310
MS.gene80300.t1 AT5G61930 43.562 365 196 6 40 401 45 402 1.14e-102 310
MS.gene80300.t1 AT3G21740 36.700 297 157 2 90 386 55 320 2.55e-58 194
MS.gene80300.t1 AT3G21740 29.457 129 91 0 252 380 45 173 3.38e-14 73.6

Find 119 sgRNAs with CRISPR-Local

Find 163 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGGCGGGAAATCGACATTT+TGG 0.188456 6.1:-59644331 None:intergenic
CTAACATGCCACACGTAATT+TGG 0.213116 6.1:-59645539 None:intergenic
AGCCTGTTTGATTTGTTTAA+AGG 0.228852 6.1:-59644390 None:intergenic
GGAACAAGCAATCCATTCTT+TGG 0.306524 6.1:-59644467 None:intergenic
TTGCATGTCATTCCTGCATA+TGG 0.315621 6.1:+59644560 MS.gene80300:CDS
TTGATTTGTTTAAAGGGAAT+AGG 0.347331 6.1:-59644383 None:intergenic
GCTGCGTTGAAGAGATATTA+TGG 0.350367 6.1:+59645587 MS.gene80300:CDS
TGACAGAGGCGGACATTTAG+AGG 0.357152 6.1:+59645202 MS.gene80300:CDS
TATCATCTCTTTGACCCATT+TGG 0.357302 6.1:+59645035 MS.gene80300:CDS
TGATTTGTTTAAAGGGAATA+GGG 0.359796 6.1:-59644382 None:intergenic
GCCTGTTTGATTTGTTTAAA+GGG 0.365584 6.1:-59644389 None:intergenic
ATAGTTCTCATGCTTGGGTC+AGG 0.375929 6.1:+59644976 MS.gene80300:CDS
TGCATAATGCGCTTACCAAA+TGG 0.376615 6.1:-59645050 None:intergenic
TCCCTTTAAACAAATCAAAC+AGG 0.403204 6.1:+59644388 MS.gene80300:CDS
GTGGATCATTTAAGCATCAA+TGG 0.408425 6.1:+59645465 MS.gene80300:CDS
TGGCATGTTAGAGACATTAA+TGG 0.410621 6.1:+59645551 MS.gene80300:CDS
TTCGTACGGGATTTGTCCTT+CGG 0.414111 6.1:-59645160 None:intergenic
CTCATGAAGTATTTGATGCT+TGG 0.427194 6.1:+59644507 MS.gene80300:CDS
CAGGAATGACATGCAAAAGC+TGG 0.432380 6.1:-59644553 None:intergenic
CTTCATGAGCAACGGGAACT+AGG 0.441984 6.1:-59644493 None:intergenic
TGCATATCAAATAGTTAGAA+AGG 0.444834 6.1:+59645349 MS.gene80300:CDS
CCTCTTGAACCTCCAAAGAA+TGG 0.447913 6.1:+59644455 MS.gene80300:CDS
TTCCCGCCAAAGTTGCCAAA+AGG 0.457458 6.1:+59644344 MS.gene80300:CDS
GGAGGTTCATGTTGGACCGT+GGG 0.464550 6.1:+59645424 MS.gene80300:CDS
TGTTTAAAGGGAATAGGGTA+TGG 0.466391 6.1:-59644377 None:intergenic
TCAAGCCGGCGTGGATATCC+CGG 0.472145 6.1:+59645124 MS.gene80300:CDS
GCATAATGCGCTTACCAAAT+GGG 0.472311 6.1:-59645049 None:intergenic
ACGTCATAGTTCTCATGCTT+GGG 0.473814 6.1:+59644971 MS.gene80300:CDS
CGTCTCCACACAAATTCGTC+AGG 0.475595 6.1:-59644296 None:intergenic
TCTCTTCAACGCAGCCGTGA+TGG 0.477116 6.1:-59645579 None:intergenic
TCAAATACTTCATGAGCAAC+GGG 0.479703 6.1:-59644500 None:intergenic
CTCTTCAACGCAGCCGTGAT+GGG 0.482385 6.1:-59645578 None:intergenic
CACGTCATAGTTCTCATGCT+TGG 0.482980 6.1:+59644970 MS.gene80300:CDS
ATTCCCGCCTCATCTGTGTC+TGG 0.482983 6.1:-59645701 None:intergenic
GCTCAACAACAGCTGGAATC+CGG 0.485227 6.1:-59645094 None:intergenic
TAGTTCTCATGCTTGGGTCA+GGG 0.486077 6.1:+59644977 MS.gene80300:CDS
GGTCACAATGCAAAGCTTTG+TGG 0.487513 6.1:+59645446 MS.gene80300:CDS
AAGTGTAGATCGGCATTACC+TGG 0.487530 6.1:+59644242 MS.gene80300:CDS
GGATTTGTCCTTCGGCGGGA+TGG 0.487968 6.1:-59645152 None:intergenic
TACGGGATTTGTCCTTCGGC+GGG 0.489443 6.1:-59645156 None:intergenic
TCCCGCCAAAGTTGCCAAAA+GGG 0.490337 6.1:+59644345 MS.gene80300:CDS
GACTTTGATACTTACCGAGC+TGG 0.500611 6.1:+59645266 MS.gene80300:CDS
CGATTTCTGCAGTGAATGCT+CGG 0.504459 6.1:+59644887 MS.gene80300:intron
GCAAGCTGGTGCACGTATAC+CGG 0.508230 6.1:+59645640 MS.gene80300:CDS
CCATTCTTTGGAGGTTCAAG+AGG 0.510935 6.1:-59644455 None:intergenic
AGATGGAAAACATGGTTGGC+AGG 0.511785 6.1:+59645490 MS.gene80300:CDS
ATGCAGAGCTCAACAACAGC+TGG 0.512811 6.1:-59645101 None:intergenic
AAGAGATGATATGACTCAAT+CGG 0.515946 6.1:-59645023 None:intergenic
ATCAAATACTTCATGAGCAA+CGG 0.518555 6.1:-59644501 None:intergenic
GAGTAATACTTTGTGCCAAT+CGG 0.519093 6.1:+59644264 MS.gene80300:CDS
TCTCCTCCCTACCATTCGTA+CGG 0.519425 6.1:-59645174 None:intergenic
TGAGCAACGGGAACTAGGTC+AGG 0.519988 6.1:-59644488 None:intergenic
AGAACTATGACGTGTTGAAC+TGG 0.520026 6.1:-59644961 None:intergenic
AGTTCTCATGCTTGGGTCAG+GGG 0.522088 6.1:+59644978 MS.gene80300:CDS
TTGCACACAAACTTCTACTA+CGG 0.527595 6.1:-59645621 None:intergenic
GGAAAGAAAACTTGCTAGCA+AGG 0.530795 6.1:+59644421 MS.gene80300:CDS
ACAAGCAATCCATTCTTTGG+AGG 0.530814 6.1:-59644464 None:intergenic
CTTCGGCGGGATGGATACTC+CGG 0.532934 6.1:-59645143 None:intergenic
ACGTCTGACAACGGTGGAGG+GGG 0.534026 6.1:-59645299 None:intergenic
GGTACGTCTGACAACGGTGG+AGG 0.534438 6.1:-59645302 None:intergenic
GAAGTACATGTGGCTCAGAC+TGG 0.537083 6.1:+59644909 MS.gene80300:CDS
ACGGCCTTCACAATCTAGAA+TGG 0.542451 6.1:-59644937 None:intergenic
CTCCTCCCTACCATTCGTAC+GGG 0.543645 6.1:-59645173 None:intergenic
ACAAATCCCGTACGAATGGT+AGG 0.543894 6.1:+59645167 MS.gene80300:CDS
CGGAGGTTCATGTTGGACCG+TGG 0.546022 6.1:+59645423 MS.gene80300:CDS
ATCAACGGCTTGTACTCGTC+CGG 0.547415 6.1:-59645659 None:intergenic
AATCAAACAGGCTGCAAAGA+CGG 0.548813 6.1:+59644400 MS.gene80300:CDS
GTTGAGCTCTGCATTCAAGC+CGG 0.549404 6.1:+59645110 MS.gene80300:CDS
TATTGCTCGGAGGTTCATGT+TGG 0.552092 6.1:+59645416 MS.gene80300:CDS
CAATGGAGAGATGGAAAACA+TGG 0.554191 6.1:+59645482 MS.gene80300:CDS
GTGTACCTACAGCCATATGC+AGG 0.556756 6.1:-59644572 None:intergenic
GAAAGCATGCGGTTATTGCT+CGG 0.560639 6.1:+59645403 MS.gene80300:CDS
GCATATCAAATAGTTAGAAA+GGG 0.563121 6.1:+59645350 MS.gene80300:CDS
ACACAAACTTCTACTACGGC+AGG 0.565853 6.1:-59645617 None:intergenic
TTTAAGCATCAATGGAGAGA+TGG 0.566672 6.1:+59645473 MS.gene80300:CDS
TACGTCTGACAACGGTGGAG+GGG 0.567599 6.1:-59645300 None:intergenic
GTCAGGTCTAAGCTTCCGAT+TGG 0.569871 6.1:-59644279 None:intergenic
ACATGCCACACGTAATTTGG+AGG 0.570824 6.1:-59645536 None:intergenic
TGCAGAATTCTCTTCTGTCA+TGG 0.573385 6.1:+59645244 MS.gene80300:CDS
CGGAGTATCCATCCCGCCGA+AGG 0.584679 6.1:+59645144 MS.gene80300:CDS
ACAATTCAACAAGTGTAGAT+CGG 0.588302 6.1:+59644232 MS.gene80300:CDS
TCATTCCTGCATATGGCTGT+AGG 0.588472 6.1:+59644567 MS.gene80300:CDS
AGGAATGACATGCAAAAGCT+GGG 0.589239 6.1:-59644552 None:intergenic
TCTGCAGCGTGCAACGACTT+AGG 0.591342 6.1:-59645227 None:intergenic
AAGGACAAATCCCGTACGAA+TGG 0.592295 6.1:+59645163 MS.gene80300:CDS
GGAGAGATGGAAAACATGGT+TGG 0.592522 6.1:+59645486 MS.gene80300:CDS
ATACGCGGTTTGAATATGAC+CGG 0.594381 6.1:+59645075 MS.gene80300:CDS
TTCAACGCAGCCGTGATGGG+AGG 0.603188 6.1:-59645575 None:intergenic
AGCTTTGCATTGTGACCCCA+CGG 0.605815 6.1:-59645440 None:intergenic
TATGACGTGTTGAACTGGTA+CGG 0.605983 6.1:-59644956 None:intergenic
CAAATCCCGTACGAATGGTA+GGG 0.608289 6.1:+59645168 MS.gene80300:CDS
ATTCCAGACACAGATGAGGC+GGG 0.610636 6.1:+59645698 MS.gene80300:CDS
GTACGGGATTTGTCCTTCGG+CGG 0.615282 6.1:-59645157 None:intergenic
GTACGTCTGACAACGGTGGA+GGG 0.615937 6.1:-59645301 None:intergenic
TTCGGCGGGATGGATACTCC+GGG 0.618728 6.1:-59645142 None:intergenic
TTGGCACAAAGTATTACTCC+AGG 0.619449 6.1:-59644260 None:intergenic
CACTCCATTCTAGATTGTGA+AGG 0.624567 6.1:+59644933 MS.gene80300:CDS
ACGAGTACAAGCCGTTGATG+AGG 0.627677 6.1:+59645663 MS.gene80300:CDS
ACAATATCAAGCCTCATCAA+CGG 0.629198 6.1:-59645674 None:intergenic
GGGGAAATCGATCACCAGCT+CGG 0.633708 6.1:-59645280 None:intergenic
AATTCCAGACACAGATGAGG+CGG 0.641140 6.1:+59645697 MS.gene80300:CDS
CATTAATGGACCTCCCATCA+CGG 0.643796 6.1:+59645565 MS.gene80300:CDS
ATCCCGTACGAATGGTAGGG+AGG 0.647760 6.1:+59645171 MS.gene80300:CDS
GTAGAAGTTTGTGTGCAAGC+TGG 0.651785 6.1:+59645626 MS.gene80300:CDS
TGAATGCTCGGAAGTACATG+TGG 0.653362 6.1:+59644899 MS.gene80300:CDS
GTTGAAGAGATATTATGGAA+AGG 0.653812 6.1:+59645592 MS.gene80300:CDS
GAAAGAAAACTTGCTAGCAA+GGG 0.657753 6.1:+59644422 MS.gene80300:CDS
AAATACACGGTGAAAGCATG+CGG 0.672006 6.1:+59645392 MS.gene80300:CDS
TGTAATTCCAGACACAGATG+AGG 0.676525 6.1:+59645694 MS.gene80300:CDS
GGGAGGAGAGTACTTGACAG+AGG 0.676607 6.1:+59645188 MS.gene80300:CDS
GAGGTTCATGTTGGACCGTG+GGG 0.679811 6.1:+59645425 MS.gene80300:CDS
GCTCTGCATTCAAGCCGGCG+TGG 0.681643 6.1:+59645115 MS.gene80300:CDS
AGGAGAGTACTTGACAGAGG+CGG 0.682036 6.1:+59645191 MS.gene80300:CDS
TTAGACCTGACGAATTTGTG+TGG 0.683473 6.1:+59644291 MS.gene80300:CDS
AGCATGCGGTTATTGCTCGG+AGG 0.699987 6.1:+59645406 MS.gene80300:CDS
TTAGGTACGTCTGACAACGG+TGG 0.710969 6.1:-59645305 None:intergenic
AGCGCATTATGCATGATACG+CGG 0.717266 6.1:+59645060 MS.gene80300:CDS
GAAGTTGATGAAGAAATACA+CGG 0.719815 6.1:+59645379 MS.gene80300:CDS
ATACTCCGGGATATCCACGC+CGG 0.731710 6.1:-59645129 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTATTTTAATACTGATGC+AGG + chr6.1:59644845-59644864 MS.gene80300:intron 20.0%
! AAAGATAAACTATTGAGAAG+GGG - chr6.1:59644188-59644207 None:intergenic 25.0%
! AATCATCGAGAAAAATAAAC+CGG + chr6.1:59644674-59644693 MS.gene80300:intron 25.0%
! ACAAAGATAAACTATTGAGA+AGG - chr6.1:59644190-59644209 None:intergenic 25.0%
! CAAAGATAAACTATTGAGAA+GGG - chr6.1:59644189-59644208 None:intergenic 25.0%
! CATAATATTCGAGAATTGTT+TGG - chr6.1:59644137-59644156 None:intergenic 25.0%
! GCATATCAAATAGTTAGAAA+GGG + chr6.1:59645350-59645369 MS.gene80300:CDS 25.0%
! TGCATATCAAATAGTTAGAA+AGG + chr6.1:59645349-59645368 MS.gene80300:CDS 25.0%
!! ATAGTTTCTTGAGCAATTTT+AGG - chr6.1:59645326-59645345 None:intergenic 25.0%
!! ATTTTTCTCACAAACCATAA+TGG - chr6.1:59644641-59644660 None:intergenic 25.0%
!!! TGAGTCTTTACTCTTTTTTT+TGG + chr6.1:59644790-59644809 MS.gene80300:intron 25.0%
!!! TGATTTGTTTAAAGGGAATA+GGG - chr6.1:59644385-59644404 None:intergenic 25.0%
!!! TTGATTTGTTTAAAGGGAAT+AGG - chr6.1:59644386-59644405 None:intergenic 25.0%
!!! TTTTTTAGTGAAGACCATTA+TGG + chr6.1:59644624-59644643 MS.gene80300:intron 25.0%
AAAACAAATGCAACGAAACT+TGG - chr6.1:59644823-59644842 None:intergenic 30.0%
AAAAGAGTAAAGACTCATTG+TGG - chr6.1:59644787-59644806 None:intergenic 30.0%
AAGAGATGATATGACTCAAT+CGG - chr6.1:59645026-59645045 None:intergenic 30.0%
ACAATTCAACAAGTGTAGAT+CGG + chr6.1:59644232-59644251 MS.gene80300:CDS 30.0%
ATCAAATACTTCATGAGCAA+CGG - chr6.1:59644504-59644523 None:intergenic 30.0%
ATCATCGAGAAAAATAAACC+GGG + chr6.1:59644675-59644694 MS.gene80300:intron 30.0%
ATCTCAATTTAACCAGGTTT+CGG - chr6.1:59644061-59644080 None:intergenic 30.0%
GCAAAAAGGAATCAAAAGTA+GGG + chr6.1:59644111-59644130 MS.gene80300:intron 30.0%
GTTGAAGAGATATTATGGAA+AGG + chr6.1:59645592-59645611 MS.gene80300:CDS 30.0%
TATGCTCAAGGATTTGAATT+GGG - chr6.1:59644027-59644046 None:intergenic 30.0%
TCCCTTTAAACAAATCAAAC+AGG + chr6.1:59644388-59644407 MS.gene80300:CDS 30.0%
TTATGCTCAAGGATTTGAAT+TGG - chr6.1:59644028-59644047 None:intergenic 30.0%
TTGAACATCTCAATTTAACC+AGG - chr6.1:59644067-59644086 None:intergenic 30.0%
!! AGCCTGTTTGATTTGTTTAA+AGG - chr6.1:59644393-59644412 None:intergenic 30.0%
!! ATTTCGAGTTTAGTTCAAGT+AGG - chr6.1:59644712-59644731 None:intergenic 30.0%
!! GAAGTTGATGAAGAAATACA+CGG + chr6.1:59645379-59645398 MS.gene80300:CDS 30.0%
!! GCCTGTTTGATTTGTTTAAA+GGG - chr6.1:59644392-59644411 None:intergenic 30.0%
!! TTTCGAGTTTAGTTCAAGTA+GGG - chr6.1:59644711-59644730 None:intergenic 30.0%
AAACATGTCACATTAGAAGG+TGG + chr6.1:59644089-59644108 MS.gene80300:intron 35.0%
AAATCCTTGAGCATAAACGT+TGG + chr6.1:59644032-59644051 MS.gene80300:intron 35.0%
AAGTAGGGATTAAATTGTCC+CGG - chr6.1:59644696-59644715 None:intergenic 35.0%
ACAATATCAAGCCTCATCAA+CGG - chr6.1:59645677-59645696 None:intergenic 35.0%
ACGAAACATGTCACATTAGA+AGG + chr6.1:59644086-59644105 MS.gene80300:intron 35.0%
ACTAGAAGATAAGCACAGTA+TGG - chr6.1:59644597-59644616 None:intergenic 35.0%
ATAATGTTCTGCTACTGCTA+GGG + chr6.1:59644735-59644754 MS.gene80300:intron 35.0%
GAGTAATACTTTGTGCCAAT+CGG + chr6.1:59644264-59644283 MS.gene80300:CDS 35.0%
GGCAAAAAGGAATCAAAAGT+AGG + chr6.1:59644110-59644129 MS.gene80300:intron 35.0%
GTGGATCATTTAAGCATCAA+TGG + chr6.1:59645465-59645484 MS.gene80300:CDS 35.0%
TATCATCTCTTTGACCCATT+TGG + chr6.1:59645035-59645054 MS.gene80300:CDS 35.0%
TCAAATACTTCATGAGCAAC+GGG - chr6.1:59644503-59644522 None:intergenic 35.0%
TGGCATGTTAGAGACATTAA+TGG + chr6.1:59645551-59645570 MS.gene80300:CDS 35.0%
TGTTTAAAGGGAATAGGGTA+TGG - chr6.1:59644380-59644399 None:intergenic 35.0%
TTGCACACAAACTTCTACTA+CGG - chr6.1:59645624-59645643 None:intergenic 35.0%
TTTAAGCATCAATGGAGAGA+TGG + chr6.1:59645473-59645492 MS.gene80300:CDS 35.0%
! CTCATGAAGTATTTGATGCT+TGG + chr6.1:59644507-59644526 MS.gene80300:CDS 35.0%
! TTTTACCTCCAAATTACGTG+TGG + chr6.1:59645531-59645550 MS.gene80300:CDS 35.0%
!! ATTTTAGGTACGTCTGACAA+CGG - chr6.1:59645311-59645330 None:intergenic 35.0%
!! GAAAGAAAACTTGCTAGCAA+GGG + chr6.1:59644422-59644441 MS.gene80300:CDS 35.0%
!! GCTTGGAAGCTTTTGATTAA+AGG + chr6.1:59644524-59644543 MS.gene80300:CDS 35.0%
!! TATGGCTTTTTCTTCCCTTT+TGG - chr6.1:59644362-59644381 None:intergenic 35.0%
AATCAAACAGGCTGCAAAGA+CGG + chr6.1:59644400-59644419 MS.gene80300:CDS 40.0%
ACAAGCAATCCATTCTTTGG+AGG - chr6.1:59644467-59644486 None:intergenic 40.0%
ACGTCATAGTTCTCATGCTT+GGG + chr6.1:59644971-59644990 MS.gene80300:CDS 40.0%
AGAACTATGACGTGTTGAAC+TGG - chr6.1:59644964-59644983 None:intergenic 40.0%
AGGAATGACATGCAAAAGCT+GGG - chr6.1:59644555-59644574 None:intergenic 40.0%
ATACGCGGTTTGAATATGAC+CGG + chr6.1:59645075-59645094 MS.gene80300:CDS 40.0%
ATTGTGGTCCTGGAGAAAAT+GGG - chr6.1:59644771-59644790 None:intergenic 40.0%
CAATGGAGAGATGGAAAACA+TGG + chr6.1:59645482-59645501 MS.gene80300:CDS 40.0%
CACATTAGAAGGTGGCAAAA+AGG + chr6.1:59644097-59644116 MS.gene80300:intron 40.0%
CACTCCATTCTAGATTGTGA+AGG + chr6.1:59644933-59644952 MS.gene80300:CDS 40.0%
CATAATGTTCTGCTACTGCT+AGG + chr6.1:59644734-59644753 MS.gene80300:intron 40.0%
CTAACATGCCACACGTAATT+TGG - chr6.1:59645542-59645561 None:intergenic 40.0%
CTGGAGAAAATGGGATAACT+TGG - chr6.1:59644762-59644781 None:intergenic 40.0%
GAAACCAACGTTTATGCTCA+AGG - chr6.1:59644039-59644058 None:intergenic 40.0%
GCATAATGCGCTTACCAAAT+GGG - chr6.1:59645052-59645071 None:intergenic 40.0%
GCTGCGTTGAAGAGATATTA+TGG + chr6.1:59645587-59645606 MS.gene80300:CDS 40.0%
GGAACAAGCAATCCATTCTT+TGG - chr6.1:59644470-59644489 None:intergenic 40.0%
TATGACGTGTTGAACTGGTA+CGG - chr6.1:59644959-59644978 None:intergenic 40.0%
TGCAGAATTCTCTTCTGTCA+TGG + chr6.1:59645244-59645263 MS.gene80300:CDS 40.0%
TGCATAATGCGCTTACCAAA+TGG - chr6.1:59645053-59645072 None:intergenic 40.0%
TGTAATTCCAGACACAGATG+AGG + chr6.1:59645694-59645713 MS.gene80300:CDS 40.0%
TTAGACCTGACGAATTTGTG+TGG + chr6.1:59644291-59644310 MS.gene80300:CDS 40.0%
TTGCATGTCATTCCTGCATA+TGG + chr6.1:59644560-59644579 MS.gene80300:CDS 40.0%
! AAATACACGGTGAAAGCATG+CGG + chr6.1:59645392-59645411 MS.gene80300:CDS 40.0%
! CAAGTTATCCCATTTTCTCC+AGG + chr6.1:59644760-59644779 MS.gene80300:intron 40.0%
! TTCTTCCCTTTTGGCAACTT+TGG - chr6.1:59644353-59644372 None:intergenic 40.0%
! TTGGCACAAAGTATTACTCC+AGG - chr6.1:59644263-59644282 None:intergenic 40.0%
!! GGAAAGAAAACTTGCTAGCA+AGG + chr6.1:59644421-59644440 MS.gene80300:CDS 40.0%
AAGGACAAATCCCGTACGAA+TGG + chr6.1:59645163-59645182 MS.gene80300:CDS 45.0%
AAGTGTAGATCGGCATTACC+TGG + chr6.1:59644242-59644261 MS.gene80300:CDS 45.0%
AATTCCAGACACAGATGAGG+CGG + chr6.1:59645697-59645716 MS.gene80300:CDS 45.0%
ACAAATCCCGTACGAATGGT+AGG + chr6.1:59645167-59645186 MS.gene80300:CDS 45.0%
ACACAAACTTCTACTACGGC+AGG - chr6.1:59645620-59645639 None:intergenic 45.0%
ACATGCCACACGTAATTTGG+AGG - chr6.1:59645539-59645558 None:intergenic 45.0%
ACGGCCTTCACAATCTAGAA+TGG - chr6.1:59644940-59644959 None:intergenic 45.0%
AGATGGAAAACATGGTTGGC+AGG + chr6.1:59645490-59645509 MS.gene80300:CDS 45.0%
AGCGCATTATGCATGATACG+CGG + chr6.1:59645060-59645079 MS.gene80300:CDS 45.0%
ATAGTTCTCATGCTTGGGTC+AGG + chr6.1:59644976-59644995 MS.gene80300:CDS 45.0%
CAAATCCCGTACGAATGGTA+GGG + chr6.1:59645168-59645187 MS.gene80300:CDS 45.0%
CACGTCATAGTTCTCATGCT+TGG + chr6.1:59644970-59644989 MS.gene80300:CDS 45.0%
CAGGAATGACATGCAAAAGC+TGG - chr6.1:59644556-59644575 None:intergenic 45.0%
CATTGTGGTCCTGGAGAAAA+TGG - chr6.1:59644772-59644791 None:intergenic 45.0%
CCTCTTGAACCTCCAAAGAA+TGG + chr6.1:59644455-59644474 MS.gene80300:CDS 45.0%
CGATTTCTGCAGTGAATGCT+CGG + chr6.1:59644887-59644906 MS.gene80300:intron 45.0%
GACTTTGATACTTACCGAGC+TGG + chr6.1:59645266-59645285 MS.gene80300:CDS 45.0%
GGAGAGATGGAAAACATGGT+TGG + chr6.1:59645486-59645505 MS.gene80300:CDS 45.0%
GGTCACAATGCAAAGCTTTG+TGG + chr6.1:59645446-59645465 MS.gene80300:CDS 45.0%
GTAAAGACTCATTGTGGTCC+TGG - chr6.1:59644781-59644800 None:intergenic 45.0%
GTAGAAGTTTGTGTGCAAGC+TGG + chr6.1:59645626-59645645 MS.gene80300:CDS 45.0%
TAGTTCTCATGCTTGGGTCA+GGG + chr6.1:59644977-59644996 MS.gene80300:CDS 45.0%
TATTGCTCGGAGGTTCATGT+TGG + chr6.1:59645416-59645435 MS.gene80300:CDS 45.0%
TCATTCCTGCATATGGCTGT+AGG + chr6.1:59644567-59644586 MS.gene80300:CDS 45.0%
TTCGTACGGGATTTGTCCTT+CGG - chr6.1:59645163-59645182 None:intergenic 45.0%
! CATTAATGGACCTCCCATCA+CGG + chr6.1:59645565-59645584 MS.gene80300:CDS 45.0%
! CCATTCTTTGGAGGTTCAAG+AGG - chr6.1:59644458-59644477 None:intergenic 45.0%
! GAAAGCATGCGGTTATTGCT+CGG + chr6.1:59645403-59645422 MS.gene80300:CDS 45.0%
! TGAATGCTCGGAAGTACATG+TGG + chr6.1:59644899-59644918 MS.gene80300:CDS 45.0%
! TGGCGGGAAATCGACATTTT+GGG - chr6.1:59644333-59644352 None:intergenic 45.0%
! TTCCCTTTTGGCAACTTTGG+CGG - chr6.1:59644350-59644369 None:intergenic 45.0%
! TTGGCGGGAAATCGACATTT+TGG - chr6.1:59644334-59644353 None:intergenic 45.0%
!! AAACGTTGGTTTCCGAAACC+TGG + chr6.1:59644046-59644065 MS.gene80300:intron 45.0%
ACGAGTACAAGCCGTTGATG+AGG + chr6.1:59645663-59645682 MS.gene80300:CDS 50.0%
AGCTTTGCATTGTGACCCCA+CGG - chr6.1:59645443-59645462 None:intergenic 50.0%
AGTTCTCATGCTTGGGTCAG+GGG + chr6.1:59644978-59644997 MS.gene80300:CDS 50.0%
ATCAACGGCTTGTACTCGTC+CGG - chr6.1:59645662-59645681 None:intergenic 50.0%
ATGCAGAGCTCAACAACAGC+TGG - chr6.1:59645104-59645123 None:intergenic 50.0%
ATTCCAGACACAGATGAGGC+GGG + chr6.1:59645698-59645717 MS.gene80300:CDS 50.0%
CGTCTCCACACAAATTCGTC+AGG - chr6.1:59644299-59644318 None:intergenic 50.0%
CTTCATGAGCAACGGGAACT+AGG - chr6.1:59644496-59644515 None:intergenic 50.0%
GAAGTACATGTGGCTCAGAC+TGG + chr6.1:59644909-59644928 MS.gene80300:CDS 50.0%
GCTCAACAACAGCTGGAATC+CGG - chr6.1:59645097-59645116 None:intergenic 50.0%
GTCAGGTCTAAGCTTCCGAT+TGG - chr6.1:59644282-59644301 None:intergenic 50.0%
GTGTACCTACAGCCATATGC+AGG - chr6.1:59644575-59644594 None:intergenic 50.0%
GTTGAGCTCTGCATTCAAGC+CGG + chr6.1:59645110-59645129 MS.gene80300:CDS 50.0%
TCCCGCCAAAGTTGCCAAAA+GGG + chr6.1:59644345-59644364 MS.gene80300:CDS 50.0%
TCTCCTCCCTACCATTCGTA+CGG - chr6.1:59645177-59645196 None:intergenic 50.0%
TGACAGAGGCGGACATTTAG+AGG + chr6.1:59645202-59645221 MS.gene80300:CDS 50.0%
TTAGGTACGTCTGACAACGG+TGG - chr6.1:59645308-59645327 None:intergenic 50.0%
TTCCCGCCAAAGTTGCCAAA+AGG + chr6.1:59644344-59644363 MS.gene80300:CDS 50.0%
! TCCCTTTTGGCAACTTTGGC+GGG - chr6.1:59644349-59644368 None:intergenic 50.0%
!! AGGAGAGTACTTGACAGAGG+CGG + chr6.1:59645191-59645210 MS.gene80300:CDS 50.0%
AGCATGCGGTTATTGCTCGG+AGG + chr6.1:59645406-59645425 MS.gene80300:CDS 55.0%
ATACTCCGGGATATCCACGC+CGG - chr6.1:59645132-59645151 None:intergenic 55.0%
ATCCCGTACGAATGGTAGGG+AGG + chr6.1:59645171-59645190 MS.gene80300:CDS 55.0%
ATTCCCGCCTCATCTGTGTC+TGG - chr6.1:59645704-59645723 None:intergenic 55.0%
CTCCTCCCTACCATTCGTAC+GGG - chr6.1:59645176-59645195 None:intergenic 55.0%
CTCTTCAACGCAGCCGTGAT+GGG - chr6.1:59645581-59645600 None:intergenic 55.0%
GAGGTTCATGTTGGACCGTG+GGG + chr6.1:59645425-59645444 MS.gene80300:CDS 55.0%
GCAAGCTGGTGCACGTATAC+CGG + chr6.1:59645640-59645659 MS.gene80300:CDS 55.0%
GGAGGTTCATGTTGGACCGT+GGG + chr6.1:59645424-59645443 MS.gene80300:CDS 55.0%
GGGGAAATCGATCACCAGCT+CGG - chr6.1:59645283-59645302 None:intergenic 55.0%
GTACGGGATTTGTCCTTCGG+CGG - chr6.1:59645160-59645179 None:intergenic 55.0%
GTACGTCTGACAACGGTGGA+GGG - chr6.1:59645304-59645323 None:intergenic 55.0%
TACGGGATTTGTCCTTCGGC+GGG - chr6.1:59645159-59645178 None:intergenic 55.0%
TACGTCTGACAACGGTGGAG+GGG - chr6.1:59645303-59645322 None:intergenic 55.0%
TCTCTTCAACGCAGCCGTGA+TGG - chr6.1:59645582-59645601 None:intergenic 55.0%
TCTGCAGCGTGCAACGACTT+AGG - chr6.1:59645230-59645249 None:intergenic 55.0%
TGAGCAACGGGAACTAGGTC+AGG - chr6.1:59644491-59644510 None:intergenic 55.0%
!! GGGAGGAGAGTACTTGACAG+AGG + chr6.1:59645188-59645207 MS.gene80300:CDS 55.0%
ACGTCTGACAACGGTGGAGG+GGG - chr6.1:59645302-59645321 None:intergenic 60.0%
CGGAGGTTCATGTTGGACCG+TGG + chr6.1:59645423-59645442 MS.gene80300:CDS 60.0%
CTTCGGCGGGATGGATACTC+CGG - chr6.1:59645146-59645165 None:intergenic 60.0%
GGATTTGTCCTTCGGCGGGA+TGG - chr6.1:59645155-59645174 None:intergenic 60.0%
GGTACGTCTGACAACGGTGG+AGG - chr6.1:59645305-59645324 None:intergenic 60.0%
TCAAGCCGGCGTGGATATCC+CGG + chr6.1:59645124-59645143 MS.gene80300:CDS 60.0%
TTCGGCGGGATGGATACTCC+GGG - chr6.1:59645145-59645164 None:intergenic 60.0%
! TTCAACGCAGCCGTGATGGG+AGG - chr6.1:59645578-59645597 None:intergenic 60.0%
GCTCTGCATTCAAGCCGGCG+TGG + chr6.1:59645115-59645134 MS.gene80300:CDS 65.0%
! CGGAGTATCCATCCCGCCGA+AGG + chr6.1:59645144-59645163 MS.gene80300:CDS 65.0%


Chromosome Type Strat End Strand Name
chr6.1 gene 59644007 59645733 59644007 ID=MS.gene80300
chr6.1 mRNA 59644007 59645733 59644007 ID=MS.gene80300.t1;Parent=MS.gene80300
chr6.1 exon 59644007 59644009 59644007 ID=MS.gene80300.t1.exon1;Parent=MS.gene80300.t1
chr6.1 CDS 59644007 59644009 59644007 ID=cds.MS.gene80300.t1;Parent=MS.gene80300.t1
chr6.1 exon 59644221 59644588 59644221 ID=MS.gene80300.t1.exon2;Parent=MS.gene80300.t1
chr6.1 CDS 59644221 59644588 59644221 ID=cds.MS.gene80300.t1;Parent=MS.gene80300.t1
chr6.1 exon 59644899 59645733 59644899 ID=MS.gene80300.t1.exon3;Parent=MS.gene80300.t1
chr6.1 CDS 59644899 59645733 59644899 ID=cds.MS.gene80300.t1;Parent=MS.gene80300.t1
Gene Sequence

>MS.gene80300

ATGGTACTTTGACTACACCCAATTCAAATCCTTGAGCATAAACGTTGGTTTCCGAAACCTGGTTAAATTGAGATGTTCAACGAAACATGTCACATTAGAAGGTGGCAAAAAGGAATCAAAAGTAGGGCCAAACAATTCTCGAATATTATGTAATGTTATTTTCTTGAAAAATTTATTTCCCCTTCTCAATAGTTTATCTTTGTCTTCGTTGCAGTTTAAGTGTCAACAATTCAACAAGTGTAGATCGGCATTACCTGGAGTAATACTTTGTGCCAATCGGAAGCTTAGACCTGACGAATTTGTGTGGAGACGAAATGAGTCAACCCAAAATGTCGATTTCCCGCCAAAGTTGCCAAAAGGGAAGAAAAAGCCATACCCTATTCCCTTTAAACAAATCAAACAGGCTGCAAAGACGGAAAGAAAACTTGCTAGCAAGGGAATAGAGAAACCTCTTGAACCTCCAAAGAATGGATTGCTTGTTCCTGACCTAGTTCCCGTTGCTCATGAAGTATTTGATGCTTGGAAGCTTTTGATTAAAGGTCTTTCCCAGCTTTTGCATGTCATTCCTGCATATGGCTGTAGGTACACCATACTGTGCTTATCTTCTAGTTTTTTATTTTTTTAGTGAAGACCATTATGGTTTGTGAGAAAAATAAAAGCAACTTTTAATCATCGAGAAAAATAAACCGGGACAATTTAATCCCTACTTGAACTAAACTCGAAATGTCATAATGTTCTGCTACTGCTAGGGACCAAGTTATCCCATTTTCTCCAGGACCACAATGAGTCTTTACTCTTTTTTTTGGTCAATGTCCAAGTTTCGTTGCATTTGTTTTCTTTTTATTTTAATACTGATGCAGGAGTTAAGATCTTGTCAACTCGATTTCTGCAGTGAATGCTCGGAAGTACATGTGGCTCAGACTGGTCACTCCATTCTAGATTGTGAAGGCCGTACCAGTTCAACACGTCATAGTTCTCATGCTTGGGTCAGGGGCAACGTCAATGATATACTTGTTCCGATTGAGTCATATCATCTCTTTGACCCATTTGGTAAGCGCATTATGCATGATACGCGGTTTGAATATGACCGGATTCCAGCTGTTGTTGAGCTCTGCATTCAAGCCGGCGTGGATATCCCGGAGTATCCATCCCGCCGAAGGACAAATCCCGTACGAATGGTAGGGAGGAGAGTACTTGACAGAGGCGGACATTTAGAGGAGCCTAAGTCGTTGCACGCTGCAGAATTCTCTTCTGTCATGGACTTTGATACTTACCGAGCTGGTGATCGATTTCCCCCTCCACCGTTGTCAGACGTACCTAAAATTGCTCAAGAAACTATCGATGCATATCAAATAGTTAGAAAGGGTGTCAAGAAGTTGATGAAGAAATACACGGTGAAAGCATGCGGTTATTGCTCGGAGGTTCATGTTGGACCGTGGGGTCACAATGCAAAGCTTTGTGGATCATTTAAGCATCAATGGAGAGATGGAAAACATGGTTGGCAGGATGCTACTCTAGATGAAGTTTTACCTCCAAATTACGTGTGGCATGTTAGAGACATTAATGGACCTCCCATCACGGCTGCGTTGAAGAGATATTATGGAAAGGCTCCTGCCGTAGTAGAAGTTTGTGTGCAAGCTGGTGCACGTATACCGGACGAGTACAAGCCGTTGATGAGGCTTGATATTGTAATTCCAGACACAGATGAGGCGGGAATGATTGCTTGA
Protein sequence

>MS.gene80300.t1

MFKCQQFNKCRSALPGVILCANRKLRPDEFVWRRNESTQNVDFPPKLPKGKKKPYPIPFKQIKQAAKTERKLASKGIEKPLEPPKNGLLVPDLVPVAHEVFDAWKLLIKGLSQLLHVIPAYGCSECSEVHVAQTGHSILDCEGRTSSTRHSSHAWVRGNVNDILVPIESYHLFDPFGKRIMHDTRFEYDRIPAVVELCIQAGVDIPEYPSRRRTNPVRMVGRRVLDRGGHLEEPKSLHAAEFSSVMDFDTYRAGDRFPPPPLSDVPKIAQETIDAYQIVRKGVKKLMKKYTVKACGYCSEVHVGPWGHNAKLCGSFKHQWRDGKHGWQDATLDEVLPPNYVWHVRDINGPPITAALKRYYGKAPAVVEVCVQAGARIPDEYKPLMRLDIVIPDTDEAGMIA