Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81556.t1 | XP_024640924.1 | 91.1 | 484 | 39 | 2 | 1 | 480 | 1 | 484 | 1.90E-166 | 595.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81556.t1 | A0A396HXU5 | 84.0 | 520 | 43 | 3 | 1 | 480 | 1 | 520 | 1.4e-158 | 569.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049159 | MS.gene81556 | 0.800564 | 1.36E-48 | -1.69E-46 |
| MS.gene050941 | MS.gene81556 | 0.815792 | 7.71E-52 | -1.69E-46 |
| MS.gene053008 | MS.gene81556 | 0.833047 | 6.67E-56 | -1.69E-46 |
| MS.gene05517 | MS.gene81556 | 0.815302 | 9.91E-52 | -1.69E-46 |
| MS.gene056831 | MS.gene81556 | 0.816324 | 5.86E-52 | -1.69E-46 |
| MS.gene059283 | MS.gene81556 | 0.849742 | 2.66E-60 | -1.69E-46 |
| MS.gene05979 | MS.gene81556 | 0.803296 | 3.73E-49 | -1.69E-46 |
| MS.gene060296 | MS.gene81556 | 0.807709 | 4.43E-50 | -1.69E-46 |
| MS.gene060803 | MS.gene81556 | 0.820725 | 5.89E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81556.t1 | AT3G43590 | 51.931 | 233 | 87 | 7 | 237 | 449 | 119 | 346 | 4.57e-74 | 246 |
| MS.gene81556.t1 | AT5G36240 | 47.664 | 107 | 52 | 1 | 343 | 449 | 21 | 123 | 7.73e-28 | 112 |
| MS.gene81556.t1 | AT4G36020 | 30.508 | 177 | 100 | 6 | 279 | 445 | 134 | 297 | 2.07e-14 | 74.7 |
| MS.gene81556.t1 | AT4G36020 | 30.508 | 177 | 100 | 6 | 279 | 445 | 134 | 297 | 2.07e-14 | 74.7 |
| MS.gene81556.t1 | AT3G43490 | 48.649 | 74 | 33 | 3 | 237 | 309 | 107 | 176 | 4.48e-13 | 70.1 |
| MS.gene81556.t1 | AT4G36020 | 29.714 | 175 | 102 | 5 | 279 | 445 | 102 | 263 | 7.87e-12 | 66.2 |
Find 97 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCATGTGACCATTCTCTTTC+TGG | 0.108306 | 5.1:-68528347 | None:intergenic |
| TGCTTGAGATGCGGAAATTC+TGG | 0.208991 | 5.1:+68526929 | MS.gene81556:CDS |
| GAGCCACCTGTGTGCTTCTT+TGG | 0.215455 | 5.1:-68526884 | None:intergenic |
| TCACCAGGTACCACTTTCTC+TGG | 0.278896 | 5.1:-68525885 | None:intergenic |
| CTGGTTGATCCTTCTGAATT+AGG | 0.282005 | 5.1:-68529761 | None:intergenic |
| TGATGGAGGATCAAAATATC+TGG | 0.299120 | 5.1:-68526069 | None:intergenic |
| TACGAGCGACAGTGTTGTTC+TGG | 0.299892 | 5.1:+68525265 | MS.gene81556:CDS |
| ATCCTCCATCAGATAATGTT+TGG | 0.299939 | 5.1:+68526081 | MS.gene81556:CDS |
| TGCTATGTGTGCAAGAAATT+TGG | 0.301951 | 5.1:+68527176 | MS.gene81556:CDS |
| TACGAGCGACAATGTTGTTC+TGG | 0.310663 | 5.1:+68524032 | MS.gene81556:CDS |
| ATCATGGGTACTCAGCTTCT+AGG | 0.334635 | 5.1:+68529792 | MS.gene81556:CDS |
| TCCACAAAACGGCGCCGTAT+TGG | 0.363467 | 5.1:+68523031 | MS.gene81556:CDS |
| TCCTCCATCAGATAATGTTT+GGG | 0.366216 | 5.1:+68526082 | MS.gene81556:CDS |
| TTGTAGCAAGAAAATTCTTT+TGG | 0.368351 | 5.1:-68527233 | None:intergenic |
| AGATCTGCACCTTATGATAA+TGG | 0.377830 | 5.1:+68528375 | MS.gene81556:CDS |
| CATGTGACCATTCTCTTTCT+GGG | 0.391712 | 5.1:-68528346 | None:intergenic |
| TCGGTGACCAGCTAGCTTGC+AGG | 0.405953 | 5.1:-68526849 | None:intergenic |
| AGTTAAAGAAGAAGAAGAAA+AGG | 0.420892 | 5.1:+68525839 | MS.gene81556:CDS |
| TAACAGAACTCGAGCATTCT+CGG | 0.424927 | 5.1:-68527562 | None:intergenic |
| CGCCTCCTCAGGTAGGCAAC+AGG | 0.425438 | 5.1:+68528289 | MS.gene81556:intron |
| AGGAGATTAAGACTAAGAAA+AGG | 0.443967 | 5.1:+68525399 | MS.gene81556:CDS |
| TTGAGAGAAGTTACAACAAC+GGG | 0.444049 | 5.1:+68529840 | MS.gene81556:CDS |
| CTTGGTTCTGCTGCTTCACC+AGG | 0.448212 | 5.1:-68525900 | None:intergenic |
| TGCTATAACTGCGGCGAAGA+AGG | 0.451788 | 5.1:+68526110 | MS.gene81556:CDS |
| CTATTGTTACCATTATCATA+AGG | 0.452102 | 5.1:-68528384 | None:intergenic |
| GGGTACTCAGCTTCTAGGTT+TGG | 0.461563 | 5.1:+68529797 | MS.gene81556:CDS |
| GCTTGAGATGCGGAAATTCT+GGG | 0.463958 | 5.1:+68526930 | MS.gene81556:CDS |
| AATCTCCTCACTATGCAATT+CGG | 0.465805 | 5.1:-68525384 | None:intergenic |
| GTCAGATGGGTCATATTGGT+TGG | 0.466738 | 5.1:+68527261 | MS.gene81556:CDS |
| AATCTCCTCACTATGTGATT+CGG | 0.470113 | 5.1:-68524151 | None:intergenic |
| TTCAACTCACAAGCAGCTTA+CGG | 0.471177 | 5.1:-68525953 | None:intergenic |
| TGTGGTCAGATGGGTCATAT+TGG | 0.473124 | 5.1:+68527257 | MS.gene81556:CDS |
| AGAGAAACATCACATATAAA+CGG | 0.475931 | 5.1:-68530773 | None:intergenic |
| TGCTCTACCTGCAAGCTAGC+TGG | 0.479633 | 5.1:+68526842 | MS.gene81556:CDS |
| AATTGGTGCAGGCATGTTCA+AGG | 0.480571 | 5.1:+68527468 | MS.gene81556:intron |
| AGAATGCTCGAGTTCTGTTA+AGG | 0.481223 | 5.1:+68527565 | MS.gene81556:CDS |
| GGCGCATGCTAAAGTGGAAA+CGG | 0.481741 | 5.1:+68523999 | MS.gene81556:CDS |
| GGCAGTTTGAGTCATAATAA+TGG | 0.485509 | 5.1:+68526188 | MS.gene81556:CDS |
| TTGTATTGACACAGCATAAG+TGG | 0.489891 | 5.1:-68527199 | None:intergenic |
| TTGCTACAAATGTGGTCAGA+TGG | 0.490204 | 5.1:+68527247 | MS.gene81556:CDS |
| CACAGACAGTAAAGAAGAAG+AGG | 0.492551 | 5.1:+68523057 | MS.gene81556:CDS |
| CACCAGGTACCACTTTCTCT+GGG | 0.492731 | 5.1:-68525884 | None:intergenic |
| AACAGAACTCGAGCATTCTC+GGG | 0.497126 | 5.1:-68527561 | None:intergenic |
| TTTGGCAACTCGAGTAGTAA+TGG | 0.502010 | 5.1:+68529815 | MS.gene81556:CDS |
| TGCTATAATTGCGGTGAACA+AGG | 0.510539 | 5.1:+68527530 | MS.gene81556:CDS |
| GCGCATGCTAAAGTGGAAAC+GGG | 0.512109 | 5.1:+68524000 | MS.gene81556:CDS |
| TTTACGCGGAATGTTCATGA+TGG | 0.518252 | 5.1:+68529734 | MS.gene81556:CDS |
| TAACAGATTCATGTCGTGAT+CGG | 0.518849 | 5.1:-68530750 | None:intergenic |
| ACGAGCGACAATGTTGTTCT+GGG | 0.519892 | 5.1:+68524033 | MS.gene81556:CDS |
| AAAAGTGGCGCATGCTAAAG+TGG | 0.520915 | 5.1:+68523993 | MS.gene81556:CDS |
| CCATGCTTCTTTCAACTGTA+CGG | 0.523223 | 5.1:+68526133 | MS.gene81556:CDS |
| CCGCAATTATAGCATGAACT+AGG | 0.523263 | 5.1:-68527521 | None:intergenic |
| ACGAGCGACAGTGTTGTTCT+GGG | 0.526116 | 5.1:+68525266 | MS.gene81556:CDS |
| CTAAGATCCCAGAAAGAGAA+TGG | 0.530781 | 5.1:+68528339 | MS.gene81556:CDS |
| TACTGTCTGTGTATCCAATA+CGG | 0.537948 | 5.1:-68523045 | None:intergenic |
| GATTGAAGACCCAGAGAAAG+TGG | 0.538571 | 5.1:+68525875 | MS.gene81556:CDS |
| TCAGATGGGTCATATTGGTT+GGG | 0.539041 | 5.1:+68527262 | MS.gene81556:CDS |
| TGCTACAAATGTGGTCAGAT+GGG | 0.541624 | 5.1:+68527248 | MS.gene81556:CDS |
| CGTGCAATCACTCCACAAAA+CGG | 0.543563 | 5.1:+68523020 | MS.gene81556:CDS |
| CTCACTATGCAATTCGGAAG+CGG | 0.544861 | 5.1:-68525378 | None:intergenic |
| TTGCTCGTATTAACCACCCT+TGG | 0.550857 | 5.1:-68525918 | None:intergenic |
| TCTTTGGACAGTCGCTACTT+CGG | 0.554717 | 5.1:-68526868 | None:intergenic |
| TGCTAAAGTGGAAACGGGCA+TGG | 0.556871 | 5.1:+68524005 | MS.gene81556:CDS |
| CCAGAAAGAGAATGGTCACA+TGG | 0.563687 | 5.1:+68528347 | MS.gene81556:CDS |
| GATGGAGGATCAAAATATCT+GGG | 0.565011 | 5.1:-68526068 | None:intergenic |
| GTTCCCCAAACATTATCTGA+TGG | 0.574988 | 5.1:-68526086 | None:intergenic |
| CAGATTCATGTCGTGATCGG+CGG | 0.575599 | 5.1:-68530747 | None:intergenic |
| TGATGATGAAGAAGCAAACA+AGG | 0.576755 | 5.1:+68522959 | MS.gene81556:CDS |
| GACCCAGAGAAAGTGGTACC+TGG | 0.583600 | 5.1:+68525882 | MS.gene81556:CDS |
| TTCATGTCGTGATCGGCGGA+TGG | 0.585796 | 5.1:-68530743 | None:intergenic |
| GATGGAACTCCTAATTCAGA+AGG | 0.593536 | 5.1:+68529752 | MS.gene81556:CDS |
| TGGTGAAGCAGCAGAACCAA+GGG | 0.595481 | 5.1:+68525902 | MS.gene81556:CDS |
| CTGGTGAAGCAGCAGAACCA+AGG | 0.600199 | 5.1:+68525901 | MS.gene81556:CDS |
| CGCTTCCGAATCACATAGTG+AGG | 0.601770 | 5.1:+68524146 | MS.gene81556:CDS |
| AAACTGCCACACACAAAACA+AGG | 0.606860 | 5.1:-68526173 | None:intergenic |
| CCCCAAACATTATCTGATGG+AGG | 0.610792 | 5.1:-68526083 | None:intergenic |
| ACTAGGTGTGGTGGCAGCAG+TGG | 0.613482 | 5.1:-68527504 | None:intergenic |
| CGCTTCCGAATTGCATAGTG+AGG | 0.623157 | 5.1:+68525379 | MS.gene81556:CDS |
| GCTCGTATCAATTTCCACCT+CGG | 0.628136 | 5.1:-68525249 | None:intergenic |
| TTATTCGCAGGATGATCTCA+AGG | 0.631797 | 5.1:+68526979 | MS.gene81556:CDS |
| CCTAGTTCATGCTATAATTG+CGG | 0.634547 | 5.1:+68527521 | MS.gene81556:CDS |
| CCGTACAGTTGAAAGAAGCA+TGG | 0.635614 | 5.1:-68526133 | None:intergenic |
| ATAGCATGAACTAGGTGTGG+TGG | 0.642456 | 5.1:-68527513 | None:intergenic |
| ATTATAGCATGAACTAGGTG+TGG | 0.648256 | 5.1:-68527516 | None:intergenic |
| AACTCACAAGCAGCTTACGG+AGG | 0.651992 | 5.1:-68525950 | None:intergenic |
| AGAGAAGTTACAACAACGGG+TGG | 0.653732 | 5.1:+68529843 | MS.gene81556:CDS |
| TGGGGAACATGCTATAACTG+CGG | 0.659213 | 5.1:+68526101 | MS.gene81556:CDS |
| GTACCCATGATGAAAAGTCC+TGG | 0.660670 | 5.1:-68529780 | None:intergenic |
| CCTCCATCAGATAATGTTTG+GGG | 0.668625 | 5.1:+68526083 | MS.gene81556:CDS |
| TGTCCAAAGAAGCACACAGG+TGG | 0.675544 | 5.1:+68526881 | MS.gene81556:CDS |
| CAGAAAGAGAATGGTCACAT+GGG | 0.678367 | 5.1:+68528348 | MS.gene81556:CDS |
| TGAAGCAGCAGAACCAAGGG+TGG | 0.678993 | 5.1:+68525905 | MS.gene81556:CDS |
| AAAAGTGGAGAATGCCGAGG+TGG | 0.683836 | 5.1:+68525235 | MS.gene81556:CDS |
| CTAATTCAGAAGGATCAACC+AGG | 0.685700 | 5.1:+68529762 | MS.gene81556:CDS |
| TTTGAGAGAAGTTACAACAA+CGG | 0.685995 | 5.1:+68529839 | MS.gene81556:CDS |
| GACTGTCCAAAGAAGCACAC+AGG | 0.690399 | 5.1:+68526878 | MS.gene81556:CDS |
| TCGACAGTATGCTTGAGATG+CGG | 0.692674 | 5.1:+68526920 | MS.gene81556:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 68522873 | 68530786 | 68522873 | ID=MS.gene81556 |
| chr5.1 | mRNA | 68522873 | 68530786 | 68522873 | ID=MS.gene81556.t1;Parent=MS.gene81556 |
| chr5.1 | exon | 68522873 | 68523112 | 68522873 | ID=MS.gene81556.t1.exon1;Parent=MS.gene81556.t1 |
| chr5.1 | CDS | 68522873 | 68523112 | 68522873 | ID=cds.MS.gene81556.t1;Parent=MS.gene81556.t1 |
| chr5.1 | exon | 68523937 | 68524054 | 68523937 | ID=MS.gene81556.t1.exon2;Parent=MS.gene81556.t1 |
| chr5.1 | CDS | 68523937 | 68524054 | 68523937 | ID=cds.MS.gene81556.t1;Parent=MS.gene81556.t1 |
| chr5.1 | exon | 68524139 | 68524230 | 68524139 | ID=MS.gene81556.t1.exon3;Parent=MS.gene81556.t1 |
| chr5.1 | CDS | 68524139 | 68524230 | 68524139 | ID=cds.MS.gene81556.t1;Parent=MS.gene81556.t1 |
| chr5.1 | exon | 68525179 | 68525287 | 68525179 | ID=MS.gene81556.t1.exon4;Parent=MS.gene81556.t1 |
| chr5.1 | CDS | 68525179 | 68525287 | 68525179 | ID=cds.MS.gene81556.t1;Parent=MS.gene81556.t1 |
| chr5.1 | exon | 68525372 | 68525463 | 68525372 | ID=MS.gene81556.t1.exon5;Parent=MS.gene81556.t1 |
| chr5.1 | CDS | 68525372 | 68525463 | 68525372 | ID=cds.MS.gene81556.t1;Parent=MS.gene81556.t1 |
| chr5.1 | exon | 68525831 | 68525965 | 68525831 | ID=MS.gene81556.t1.exon6;Parent=MS.gene81556.t1 |
| chr5.1 | CDS | 68525831 | 68525965 | 68525831 | ID=cds.MS.gene81556.t1;Parent=MS.gene81556.t1 |
| chr5.1 | exon | 68526062 | 68526226 | 68526062 | ID=MS.gene81556.t1.exon7;Parent=MS.gene81556.t1 |
| chr5.1 | CDS | 68526062 | 68526226 | 68526062 | ID=cds.MS.gene81556.t1;Parent=MS.gene81556.t1 |
| chr5.1 | exon | 68526833 | 68527000 | 68526833 | ID=MS.gene81556.t1.exon8;Parent=MS.gene81556.t1 |
| chr5.1 | CDS | 68526833 | 68527000 | 68526833 | ID=cds.MS.gene81556.t1;Parent=MS.gene81556.t1 |
| chr5.1 | exon | 68527167 | 68527283 | 68527167 | ID=MS.gene81556.t1.exon9;Parent=MS.gene81556.t1 |
| chr5.1 | CDS | 68527167 | 68527283 | 68527167 | ID=cds.MS.gene81556.t1;Parent=MS.gene81556.t1 |
| chr5.1 | exon | 68527479 | 68527586 | 68527479 | ID=MS.gene81556.t1.exon10;Parent=MS.gene81556.t1 |
| chr5.1 | CDS | 68527479 | 68527586 | 68527479 | ID=cds.MS.gene81556.t1;Parent=MS.gene81556.t1 |
| chr5.1 | exon | 68528300 | 68528396 | 68528300 | ID=MS.gene81556.t1.exon11;Parent=MS.gene81556.t1 |
| chr5.1 | CDS | 68528300 | 68528396 | 68528300 | ID=cds.MS.gene81556.t1;Parent=MS.gene81556.t1 |
| chr5.1 | exon | 68529706 | 68529864 | 68529706 | ID=MS.gene81556.t1.exon12;Parent=MS.gene81556.t1 |
| chr5.1 | CDS | 68529706 | 68529864 | 68529706 | ID=cds.MS.gene81556.t1;Parent=MS.gene81556.t1 |
| chr5.1 | exon | 68530725 | 68530786 | 68530725 | ID=MS.gene81556.t1.exon13;Parent=MS.gene81556.t1 |
| chr5.1 | CDS | 68530725 | 68530786 | 68530725 | ID=cds.MS.gene81556.t1;Parent=MS.gene81556.t1 |
| Gene Sequence |
| Protein sequence |
