Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81746.t1 | KEH42025.1 | 69.6 | 115 | 28 | 1 | 27 | 141 | 10 | 117 | 4.60E-32 | 147.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81746.t1 | Q9LXJ1 | 69.4 | 62 | 18 | 1 | 75 | 136 | 64 | 124 | 9.5e-18 | 91.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81746.t1 | A0A072VV98 | 69.6 | 115 | 28 | 1 | 27 | 141 | 10 | 117 | 3.3e-32 | 147.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050670 | MS.gene81746 | 0.816373 | 5.72E-52 | -1.69E-46 |
| MS.gene05105 | MS.gene81746 | 0.802333 | 5.90E-49 | -1.69E-46 |
| MS.gene051438 | MS.gene81746 | 0.820248 | 7.58E-53 | -1.69E-46 |
| MS.gene052414 | MS.gene81746 | 0.821316 | 4.31E-53 | -1.69E-46 |
| MS.gene052421 | MS.gene81746 | 0.816013 | 6.88E-52 | -1.69E-46 |
| MS.gene053449 | MS.gene81746 | 0.832205 | 1.08E-55 | -1.69E-46 |
| MS.gene055539 | MS.gene81746 | 0.815343 | 9.71E-52 | -1.69E-46 |
| MS.gene055741 | MS.gene81746 | 0.818748 | 1.67E-52 | -1.69E-46 |
| MS.gene055744 | MS.gene81746 | 0.818606 | 1.80E-52 | -1.69E-46 |
| MS.gene055909 | MS.gene81746 | 0.813494 | 2.49E-51 | -1.69E-46 |
| MS.gene055911 | MS.gene81746 | 0.811956 | 5.40E-51 | -1.69E-46 |
| MS.gene056511 | MS.gene81746 | 0.818562 | 1.84E-52 | -1.69E-46 |
| MS.gene05664 | MS.gene81746 | 0.814926 | 1.20E-51 | -1.69E-46 |
| MS.gene057734 | MS.gene81746 | 0.804618 | 1.98E-49 | -1.69E-46 |
| MS.gene058007 | MS.gene81746 | 0.809883 | 1.52E-50 | -1.69E-46 |
| MS.gene058075 | MS.gene81746 | 0.800488 | 1.41E-48 | -1.69E-46 |
| MS.gene058120 | MS.gene81746 | 0.807172 | 5.75E-50 | -1.69E-46 |
| MS.gene058376 | MS.gene81746 | 0.801768 | 7.70E-49 | -1.69E-46 |
| MS.gene058700 | MS.gene81746 | 0.801016 | 1.10E-48 | -1.69E-46 |
| MS.gene059455 | MS.gene81746 | 0.808566 | 2.91E-50 | -1.69E-46 |
| MS.gene059674 | MS.gene81746 | 0.803456 | 3.46E-49 | -1.69E-46 |
| MS.gene059807 | MS.gene81746 | 0.827612 | 1.42E-54 | -1.69E-46 |
| MS.gene060372 | MS.gene81746 | 0.809039 | 2.30E-50 | -1.69E-46 |
| MS.gene060403 | MS.gene81746 | 0.802504 | 5.44E-49 | -1.69E-46 |
| MS.gene061154 | MS.gene81746 | 0.800322 | 1.52E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81746.t1 | MTR_1g059990 | 80.645 | 124 | 17 | 1 | 18 | 141 | 1 | 117 | 1.48e-69 | 205 |
| MS.gene81746.t1 | MTR_7g104540 | 41.975 | 162 | 50 | 5 | 18 | 136 | 1 | 161 | 1.60e-30 | 108 |
| MS.gene81746.t1 | MTR_2g086480 | 53.226 | 62 | 28 | 1 | 73 | 133 | 71 | 132 | 4.32e-14 | 67.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81746.t1 | AT3G52740 | 69.355 | 62 | 18 | 1 | 75 | 136 | 64 | 124 | 1.73e-25 | 94.4 |
| MS.gene81746.t1 | AT3G44450 | 38.776 | 98 | 52 | 2 | 37 | 134 | 7 | 96 | 7.14e-17 | 71.6 |
Find 26 sgRNAs with CRISPR-Local
Find 25 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTTCAATTCAATATCAATT+TGG | 0.251682 | 1.3:+43494408 | None:intergenic |
| AGGATTCAGATTCCACTATA+GGG | 0.317159 | 1.3:-43494086 | None:intergenic |
| CTTTCTTCTTCCTCAACAAT+AGG | 0.318419 | 1.3:+43494287 | None:intergenic |
| ACTCAAAGATAACAATATTA+AGG | 0.326492 | 1.3:-43494375 | MS.gene81746:CDS |
| TCTTCTTGTCCCCACATATC+TGG | 0.329231 | 1.3:+43494209 | None:intergenic |
| CTTTGGTTCAAGAAGCTACT+AGG | 0.363188 | 1.3:-43494106 | MS.gene81746:CDS |
| TAGGATTCAGATTCCACTAT+AGG | 0.365611 | 1.3:-43494087 | None:intergenic |
| TTGAAATGGCTGGAAGAGTA+TGG | 0.372715 | 1.3:-43494235 | MS.gene81746:CDS |
| TGTTATCTTTCATGCAAAAC+AGG | 0.376927 | 1.3:+43494486 | None:intergenic |
| TGTGACTGCTAGAACAGCTT+TGG | 0.411343 | 1.3:-43494123 | MS.gene81746:CDS |
| GAAGAGACATAGAGTTGAAA+TGG | 0.437286 | 1.3:-43494249 | MS.gene81746:CDS |
| AAGAAGAGTACTTGAAGGAT+TGG | 0.465508 | 1.3:-43494193 | MS.gene81746:CDS |
| CATGAGATTTAGTAGATGAT+TGG | 0.476408 | 1.3:+43494432 | None:intergenic |
| AGACATAGAGTTGAAATGGC+TGG | 0.496362 | 1.3:-43494245 | MS.gene81746:CDS |
| GAGTATGGATTCCAGATATG+TGG | 0.497888 | 1.3:-43494220 | MS.gene81746:CDS |
| AGTATGGATTCCAGATATGT+GGG | 0.543345 | 1.3:-43494219 | MS.gene81746:CDS |
| TTGTTATCTTTGAGTTGATG+AGG | 0.550414 | 1.3:+43494383 | None:intergenic |
| TGAAATTGCATATATGAAGA+AGG | 0.550557 | 1.3:-43494318 | MS.gene81746:CDS |
| GGATTCAGATTCCACTATAG+GGG | 0.560164 | 1.3:-43494085 | None:intergenic |
| AAGAAGAAAGTGGAAGAGAG+AGG | 0.561315 | 1.3:-43494274 | MS.gene81746:CDS |
| GGGACAAGAAGAGTACTTGA+AGG | 0.578419 | 1.3:-43494198 | MS.gene81746:CDS |
| AATTGCATATATGAAGAAGG+TGG | 0.595793 | 1.3:-43494315 | MS.gene81746:CDS |
| ATTGTTGAGGAAGAAGAAAG+TGG | 0.598074 | 1.3:-43494284 | MS.gene81746:CDS |
| TTACTAGCAGACATCAAAGG+AGG | 0.660315 | 1.3:+43494149 | None:intergenic |
| GTATGGATTCCAGATATGTG+GGG | 0.706622 | 1.3:-43494218 | MS.gene81746:CDS |
| TGATTGGTGAGTCATTGTAG+TGG | 0.742104 | 1.3:+43494448 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACTCAAAGATAACAATATTA+AGG | - | chr1.3:43494204-43494223 | MS.gene81746:CDS | 20.0% |
| !! | TCTTCAATTCAATATCAATT+TGG | + | chr1.3:43494174-43494193 | None:intergenic | 20.0% |
| ! | TGAAATTGCATATATGAAGA+AGG | - | chr1.3:43494261-43494280 | MS.gene81746:CDS | 25.0% |
| AATTGCATATATGAAGAAGG+TGG | - | chr1.3:43494264-43494283 | MS.gene81746:CDS | 30.0% | |
| CATGAGATTTAGTAGATGAT+TGG | + | chr1.3:43494150-43494169 | None:intergenic | 30.0% | |
| TGTTATCTTTCATGCAAAAC+AGG | + | chr1.3:43494096-43494115 | None:intergenic | 30.0% | |
| ! | ATTTTACTAGCAGACATCAA+AGG | + | chr1.3:43494436-43494455 | None:intergenic | 30.0% |
| !! | TTGTTATCTTTGAGTTGATG+AGG | + | chr1.3:43494199-43494218 | None:intergenic | 30.0% |
| AGTATGGATTCCAGATATGT+GGG | - | chr1.3:43494360-43494379 | MS.gene81746:CDS | 35.0% | |
| ATTGTTGAGGAAGAAGAAAG+TGG | - | chr1.3:43494295-43494314 | MS.gene81746:CDS | 35.0% | |
| CTTTCTTCTTCCTCAACAAT+AGG | + | chr1.3:43494295-43494314 | None:intergenic | 35.0% | |
| GAAGAGACATAGAGTTGAAA+TGG | - | chr1.3:43494330-43494349 | MS.gene81746:CDS | 35.0% | |
| !! | AAGAAGAGTACTTGAAGGAT+TGG | - | chr1.3:43494386-43494405 | MS.gene81746:CDS | 35.0% |
| AAGAAGAAAGTGGAAGAGAG+AGG | - | chr1.3:43494305-43494324 | MS.gene81746:CDS | 40.0% | |
| AGACATAGAGTTGAAATGGC+TGG | - | chr1.3:43494334-43494353 | MS.gene81746:CDS | 40.0% | |
| GAGTATGGATTCCAGATATG+TGG | - | chr1.3:43494359-43494378 | MS.gene81746:CDS | 40.0% | |
| GTATGGATTCCAGATATGTG+GGG | - | chr1.3:43494361-43494380 | MS.gene81746:CDS | 40.0% | |
| TGATTGGTGAGTCATTGTAG+TGG | + | chr1.3:43494134-43494153 | None:intergenic | 40.0% | |
| TTACTAGCAGACATCAAAGG+AGG | + | chr1.3:43494433-43494452 | None:intergenic | 40.0% | |
| TTGAAATGGCTGGAAGAGTA+TGG | - | chr1.3:43494344-43494363 | MS.gene81746:CDS | 40.0% | |
| ! | CTTTGGTTCAAGAAGCTACT+AGG | - | chr1.3:43494473-43494492 | MS.gene81746:CDS | 40.0% |
| TCTTCTTGTCCCCACATATC+TGG | + | chr1.3:43494373-43494392 | None:intergenic | 45.0% | |
| TGTGACTGCTAGAACAGCTT+TGG | - | chr1.3:43494456-43494475 | MS.gene81746:CDS | 45.0% | |
| !! | GGGACAAGAAGAGTACTTGA+AGG | - | chr1.3:43494381-43494400 | MS.gene81746:CDS | 45.0% |
| !! | GGTGGTTTTGCCTATTGTTG+AGG | - | chr1.3:43494282-43494301 | MS.gene81746:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 43494088 | 43494513 | 43494088 | ID=MS.gene81746 |
| chr1.3 | mRNA | 43494088 | 43494513 | 43494088 | ID=MS.gene81746.t1;Parent=MS.gene81746 |
| chr1.3 | exon | 43494088 | 43494513 | 43494088 | ID=MS.gene81746.t1.exon1;Parent=MS.gene81746.t1 |
| chr1.3 | CDS | 43494088 | 43494513 | 43494088 | ID=cds.MS.gene81746.t1;Parent=MS.gene81746.t1 |
| Gene Sequence |
| Protein sequence |
