AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene81802


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene81802.t1 MTR_5g076440 87.778 270 33 0 1 270 615 884 1.33e-161 472
MS.gene81802.t1 MTR_5g076500 88.764 267 13 1 1 267 601 850 2.11e-158 462
MS.gene81802.t1 MTR_5g076310 82.463 268 46 1 1 267 581 848 2.40e-156 457
MS.gene81802.t1 MTR_5g076380 77.528 267 60 0 1 267 604 870 6.93e-146 431
MS.gene81802.t1 MTR_3g064190 71.111 270 78 0 1 270 605 874 2.45e-133 399
MS.gene81802.t1 MTR_5g076320 72.137 262 63 1 1 262 629 880 8.09e-130 389
MS.gene81802.t1 MTR_5g076330 70.412 267 78 1 1 267 601 866 4.01e-129 388
MS.gene81802.t1 MTR_5g076300 68.539 267 83 1 1 267 626 891 6.09e-127 382
MS.gene81802.t1 MTR_3g064210 54.545 264 115 2 1 259 683 946 4.17e-92 294
MS.gene81802.t1 MTR_0189s0030 51.822 247 117 2 7 252 601 846 2.93e-80 264
MS.gene81802.t1 MTR_3g436400 48.638 257 126 3 7 258 622 877 6.96e-79 260
MS.gene81802.t1 MTR_1g052700 36.481 233 144 4 1 232 78 307 7.57e-43 149
MS.gene81802.t1 MTR_5g053930 37.662 231 140 4 1 230 524 751 7.03e-41 150
MS.gene81802.t1 MTR_8g020390 36.957 230 133 7 7 231 540 762 4.06e-40 148
MS.gene81802.t1 MTR_8g020390 37.021 235 136 7 2 231 823 1050 6.62e-40 147
MS.gene81802.t1 MTR_8g020390 37.021 235 136 7 2 231 1040 1267 1.12e-39 147
MS.gene81802.t1 MTR_2g105760 37.553 237 132 5 1 225 1835 2067 7.96e-38 142
MS.gene81802.t1 MTR_5g013820 33.884 242 140 5 1 226 668 905 2.34e-33 129
MS.gene81802.t1 MTR_5g013820 33.884 242 140 5 1 226 668 905 2.35e-33 129
MS.gene81802.t1 MTR_5g080800 52.727 110 47 1 141 250 6 110 1.17e-32 127
MS.gene81802.t1 MTR_7g092200 33.198 247 122 8 7 232 417 641 7.81e-23 98.2
MS.gene81802.t1 MTR_5g006890 32.340 235 128 9 2 218 586 807 1.40e-19 88.6
MS.gene81802.t1 MTR_2g049990 32.340 235 128 9 2 218 586 807 1.58e-19 88.6
MS.gene81802.t1 MTR_5g006890 32.340 235 128 9 2 218 327 548 2.08e-19 88.2
MS.gene81802.t1 MTR_2g049990 32.340 235 128 9 2 218 327 548 2.68e-19 87.8
MS.gene81802.t1 MTR_1g040155 30.837 227 129 9 7 215 663 879 7.54e-14 71.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene81802.t1 AT5G37165 55.513 263 110 2 2 262 159 416 8.29e-102 303
MS.gene81802.t1 AT5G52090 59.677 248 97 2 1 246 409 655 2.93e-101 310
MS.gene81802.t1 AT5G37030 60.976 246 90 3 3 243 392 636 4.84e-101 308
MS.gene81802.t1 AT1G65810 59.751 241 96 1 1 241 598 837 8.04e-99 311
MS.gene81802.t1 AT5G37140 57.724 246 104 0 1 246 426 671 1.07e-98 303
MS.gene81802.t1 AT1G65810 59.751 241 96 1 1 241 598 837 1.60e-98 310
MS.gene81802.t1 AT1G65810 59.751 241 96 1 1 241 598 837 1.94e-98 310
MS.gene81802.t1 AT5G37150 58.468 248 100 2 1 246 572 818 4.68e-98 305
MS.gene81802.t1 AT4G05540 65.116 215 72 2 1 213 476 689 4.21e-92 286
MS.gene81802.t1 AT1G65780 56.031 257 110 2 1 254 609 865 8.36e-81 263
MS.gene81802.t1 AT4G15570 36.564 227 139 4 1 225 439 662 1.21e-41 152
MS.gene81802.t1 AT4G15570 36.564 227 139 4 1 225 439 662 1.21e-41 152
MS.gene81802.t1 AT4G15570 36.564 227 139 4 1 225 528 751 1.42e-41 152
MS.gene81802.t1 AT4G15570 36.564 227 139 4 1 225 528 751 1.42e-41 152
MS.gene81802.t1 AT2G19120 37.657 239 129 8 2 231 820 1047 2.67e-39 146
MS.gene81802.t1 AT4G30100 36.522 230 126 8 7 227 988 1206 7.80e-36 136
MS.gene81802.t1 AT4G30100 36.522 230 126 8 7 227 988 1206 7.80e-36 136
MS.gene81802.t1 AT1G16800 36.161 224 131 5 10 225 1702 1921 8.88e-35 133
MS.gene81802.t1 AT1G16800 36.161 224 131 5 10 225 1772 1991 9.53e-35 133
MS.gene81802.t1 AT1G16800 36.161 224 131 5 10 225 1660 1879 9.94e-35 133
MS.gene81802.t1 AT5G47010 33.471 242 141 5 1 226 680 917 7.15e-33 127
MS.gene81802.t1 AT2G03270 33.906 233 111 8 7 218 412 622 9.56e-22 95.1
MS.gene81802.t1 AT1G05460 34.874 238 119 8 2 218 587 809 2.94e-20 90.9
MS.gene81802.t1 AT1G05460 34.874 238 119 8 2 218 587 809 1.09e-19 89.4
MS.gene81802.t1 AT5G35970 31.140 228 131 8 7 217 711 929 4.09e-16 78.6

Find 39 sgRNAs with CRISPR-Local

Find 49 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTAATGTTCGTTCTGTTGA+TGG 0.262048 5.2:+11188652 MS.gene81802:CDS
AGATATTGTCTTTGGATATT+AGG 0.300734 5.2:+11188872 MS.gene81802:intron
TCATAAACTTGAGCATTATA+TGG 0.306286 5.2:-11188577 None:intergenic
CAAGGAAACAAGCTTATTGA+TGG 0.334295 5.2:-11188193 None:intergenic
ATTGCGGACAATTGTCGATT+TGG 0.348564 5.2:+11188097 MS.gene81802:CDS
TAGCCAGTAGTGATTCTATC+TGG 0.363659 5.2:+11188909 MS.gene81802:CDS
TGTCTTGCTTAACTTTCTCC+TGG 0.405353 5.2:-11188603 None:intergenic
TATAATAAGAGCTTTCTTGA+AGG 0.419051 5.2:+11188271 MS.gene81802:CDS
ATATCTACTGTGAGATCAAA+TGG 0.422646 5.2:+11188706 MS.gene81802:CDS
TGCTGACGAGGACATGAAAC+TGG 0.441494 5.2:+11188976 MS.gene81802:CDS
CGTTCTGTTGATGGTTTCCA+AGG 0.443849 5.2:+11188661 MS.gene81802:CDS
TCAAATGGAAGTGGTAATAT+CGG 0.454389 5.2:+11188721 MS.gene81802:CDS
AAGAAGTATGTTTGAGAGAC+TGG 0.461971 5.2:+11188120 MS.gene81802:CDS
TAATATCTTCCTCACCGCCT+TGG 0.472392 5.2:-11188678 None:intergenic
TGCTCAAGTTTATGAAATCC+AGG 0.488265 5.2:+11188585 MS.gene81802:CDS
ACCAAATGTCTCCTCACCCT+TGG 0.492957 5.2:-11188329 None:intergenic
TCCAAGGGTGAGGAGACATT+TGG 0.494736 5.2:+11188328 MS.gene81802:CDS
AGCTTTCATCCTTGACAGCT+AGG 0.496236 5.2:-11188249 None:intergenic
AGCTTATTGATGGATGCATT+CGG 0.510495 5.2:-11188183 None:intergenic
GCTTTCATCCTTGACAGCTA+GGG 0.513960 5.2:-11188248 None:intergenic
TTTAATCATCTCAGAAATGA+CGG 0.516197 5.2:-11188386 None:intergenic
TATTAATATATCCAAGGGTG+AGG 0.528313 5.2:+11188318 MS.gene81802:CDS
ACAAAGAAGAAAGTTAGCAT+TGG 0.535667 5.2:+11188544 MS.gene81802:CDS
TTCGGATGCCCTAGCTGTCA+AGG 0.542296 5.2:+11188240 MS.gene81802:CDS
GAAAGTTAAGCAAGACACTT+GGG 0.544723 5.2:+11188609 MS.gene81802:CDS
CGGACATAGCTTGAAGAACA+TGG 0.553176 5.2:+11188354 MS.gene81802:CDS
GAGCTAATGTTGCAATGACA+AGG 0.555800 5.2:+11188764 MS.gene81802:CDS
AGAAAGTTAAGCAAGACACT+TGG 0.566618 5.2:+11188608 MS.gene81802:CDS
ATGTTGCAATGACAAGGGCT+AGG 0.572309 5.2:+11188770 MS.gene81802:CDS
ACTGTGAGATCAAATGGAAG+TGG 0.575364 5.2:+11188712 MS.gene81802:CDS
TCATCATAGAACTCTTTGCA+AGG 0.575937 5.2:-11188211 None:intergenic
TCTCCAGATAGAATCACTAC+TGG 0.590428 5.2:-11188912 None:intergenic
TCTGTTGATGGTTTCCAAGG+CGG 0.607165 5.2:+11188664 MS.gene81802:CDS
CTGTTATCATAATGCTGACG+AGG 0.607604 5.2:+11188964 MS.gene81802:CDS
TTTGAGAGACTGGTGATGCT+AGG 0.620623 5.2:+11188130 MS.gene81802:CDS
ATTGATGCTATACTTCCTCA+AGG 0.637505 5.2:+11189091 MS.gene81802:CDS
GGTGAGGAGACATTTGGTCG+CGG 0.639719 5.2:+11188334 MS.gene81802:CDS
AGCTAATGTTGCAATGACAA+GGG 0.653965 5.2:+11188765 MS.gene81802:CDS
TGATGGTTTCCAAGGCGGTG+AGG 0.670301 5.2:+11188669 MS.gene81802:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAAAGATAATTAACACAAA+TGG - chr5.2:11188440-11188459 None:intergenic 15.0%
!!! TCTTTTATTAATATATCCAA+GGG + chr5.2:11188313-11188332 MS.gene81802:CDS 15.0%
!!! TTCTTTTATTAATATATCCA+AGG + chr5.2:11188312-11188331 MS.gene81802:CDS 15.0%
!! ATGATTAAAAACCTAAGAAA+AGG + chr5.2:11188400-11188419 MS.gene81802:CDS 20.0%
!! GTTCTATGATGAAAAAATTT+CGG + chr5.2:11188222-11188241 MS.gene81802:CDS 20.0%
! AGATATTGTCTTTGGATATT+AGG + chr5.2:11188872-11188891 MS.gene81802:intron 25.0%
! TATAATAAGAGCTTTCTTGA+AGG + chr5.2:11188271-11188290 MS.gene81802:CDS 25.0%
! TCATAAACTTGAGCATTATA+TGG - chr5.2:11188580-11188599 None:intergenic 25.0%
! TTTAATCATCTCAGAAATGA+CGG - chr5.2:11188389-11188408 None:intergenic 25.0%
!! AGAAAAAACAACCTTTTCTT+AGG - chr5.2:11188414-11188433 None:intergenic 25.0%
!!! ACTTTACTTTGTTATGCTTT+TGG + chr5.2:11188507-11188526 MS.gene81802:intron 25.0%
ACAAAGAAGAAAGTTAGCAT+TGG + chr5.2:11188544-11188563 MS.gene81802:CDS 30.0%
ATATCTACTGTGAGATCAAA+TGG + chr5.2:11188706-11188725 MS.gene81802:CDS 30.0%
TATTAATATATCCAAGGGTG+AGG + chr5.2:11188318-11188337 MS.gene81802:CDS 30.0%
TCAAATGGAAGTGGTAATAT+CGG + chr5.2:11188721-11188740 MS.gene81802:CDS 30.0%
TGTATTTGCAGATTCAAAGT+TGG + chr5.2:11188823-11188842 MS.gene81802:intron 30.0%
! TTTTGTGCAGATATTGTCTT+TGG + chr5.2:11188864-11188883 MS.gene81802:intron 30.0%
AGAAAGTTAAGCAAGACACT+TGG + chr5.2:11188608-11188627 MS.gene81802:CDS 35.0%
AGCTAATGTTGCAATGACAA+GGG + chr5.2:11188765-11188784 MS.gene81802:CDS 35.0%
CAAGGAAACAAGCTTATTGA+TGG - chr5.2:11188196-11188215 None:intergenic 35.0%
GAAAGTTAAGCAAGACACTT+GGG + chr5.2:11188609-11188628 MS.gene81802:CDS 35.0%
GTTAATGTTCGTTCTGTTGA+TGG + chr5.2:11188652-11188671 MS.gene81802:CDS 35.0%
TCATCATAGAACTCTTTGCA+AGG - chr5.2:11188214-11188233 None:intergenic 35.0%
TGCTCAAGTTTATGAAATCC+AGG + chr5.2:11188585-11188604 MS.gene81802:CDS 35.0%
! AAGAAGTATGTTTGAGAGAC+TGG + chr5.2:11188120-11188139 MS.gene81802:CDS 35.0%
! AGCTTATTGATGGATGCATT+CGG - chr5.2:11188186-11188205 None:intergenic 35.0%
! TGCTTTTGGTAGAGTTTATG+AGG + chr5.2:11188521-11188540 MS.gene81802:intron 35.0%
ACTGTGAGATCAAATGGAAG+TGG + chr5.2:11188712-11188731 MS.gene81802:CDS 40.0%
CTGTTATCATAATGCTGACG+AGG + chr5.2:11188964-11188983 MS.gene81802:CDS 40.0%
GAGCTAATGTTGCAATGACA+AGG + chr5.2:11188764-11188783 MS.gene81802:CDS 40.0%
TAGCCAGTAGTGATTCTATC+TGG + chr5.2:11188909-11188928 MS.gene81802:CDS 40.0%
TCTCCAGATAGAATCACTAC+TGG - chr5.2:11188915-11188934 None:intergenic 40.0%
TGAATCTGCAAATACACACG+AGG - chr5.2:11188819-11188838 None:intergenic 40.0%
TGTCTTGCTTAACTTTCTCC+TGG - chr5.2:11188606-11188625 None:intergenic 40.0%
! ATTGCGGACAATTGTCGATT+TGG + chr5.2:11188097-11188116 MS.gene81802:CDS 40.0%
AGCTTTCATCCTTGACAGCT+AGG - chr5.2:11188252-11188271 None:intergenic 45.0%
ATGTTGCAATGACAAGGGCT+AGG + chr5.2:11188770-11188789 MS.gene81802:CDS 45.0%
CGGACATAGCTTGAAGAACA+TGG + chr5.2:11188354-11188373 MS.gene81802:CDS 45.0%
GCTTTCATCCTTGACAGCTA+GGG - chr5.2:11188251-11188270 None:intergenic 45.0%
TAATATCTTCCTCACCGCCT+TGG - chr5.2:11188681-11188700 None:intergenic 45.0%
! TTTGAGAGACTGGTGATGCT+AGG + chr5.2:11188130-11188149 MS.gene81802:CDS 45.0%
!! CGTTCTGTTGATGGTTTCCA+AGG + chr5.2:11188661-11188680 MS.gene81802:CDS 45.0%
!! TCTGTTGATGGTTTCCAAGG+CGG + chr5.2:11188664-11188683 MS.gene81802:CDS 45.0%
ACCAAATGTCTCCTCACCCT+TGG - chr5.2:11188332-11188351 None:intergenic 50.0%
TCCAAGGGTGAGGAGACATT+TGG + chr5.2:11188328-11188347 MS.gene81802:CDS 50.0%
TGCTGACGAGGACATGAAAC+TGG + chr5.2:11188976-11188995 MS.gene81802:CDS 50.0%
GGTGAGGAGACATTTGGTCG+CGG + chr5.2:11188334-11188353 MS.gene81802:CDS 55.0%
TGATGGTTTCCAAGGCGGTG+AGG + chr5.2:11188669-11188688 MS.gene81802:CDS 55.0%
TTCGGATGCCCTAGCTGTCA+AGG + chr5.2:11188240-11188259 MS.gene81802:CDS 55.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 11188097 11189112 11188097 ID=MS.gene81802
chr5.2 mRNA 11188097 11189112 11188097 ID=MS.gene81802.t1;Parent=MS.gene81802
chr5.2 exon 11188097 11188421 11188097 ID=MS.gene81802.t1.exon1;Parent=MS.gene81802.t1
chr5.2 CDS 11188097 11188421 11188097 ID=cds.MS.gene81802.t1;Parent=MS.gene81802.t1
chr5.2 exon 11188533 11188791 11188533 ID=MS.gene81802.t1.exon2;Parent=MS.gene81802.t1
chr5.2 CDS 11188533 11188791 11188533 ID=cds.MS.gene81802.t1;Parent=MS.gene81802.t1
chr5.2 exon 11188874 11189112 11188874 ID=MS.gene81802.t1.exon3;Parent=MS.gene81802.t1
chr5.2 CDS 11188874 11189112 11188874 ID=cds.MS.gene81802.t1;Parent=MS.gene81802.t1
Gene Sequence

>MS.gene81802

ATTGCGGACAATTGTCGATTTGGAAGAAGTATGTTTGAGAGACTGGTGATGCTAGGATACAAGAAGCATATGCTTAATGTTCAGTACCGAATGCATCCATCAATAAGCTTGTTTCCTTGCAAAGAGTTCTATGATGAAAAAATTTCGGATGCCCTAGCTGTCAAGGATGAAAGCTATAATAAGAGCTTTCTTGAAGGAGAAATGTATTCTTCTTATTCTTTTATTAATATATCCAAGGGTGAGGAGACATTTGGTCGCGGACATAGCTTGAAGAACATGGTTGAAGTTGCCGTCATTTCTGAGATGATTAAAAACCTAAGAAAAGGTTGTTTTTTCTTTACCATTTGTGTTAATTATCTTTAATAAGAAATCATCATCTTTAGTATGCATTCTGCATTCATATGTTTTACACTTTACTTTGTTATGCTTTTGGTAGAGTTTATGAGGACAAAGAAGAAAGTTAGCATTGGAATAATATCTCCATATAATGCTCAAGTTTATGAAATCCAGGAGAAAGTTAAGCAAGACACTTGGGATTCTAATTCTGACTTTTCTGTTAATGTTCGTTCTGTTGATGGTTTCCAAGGCGGTGAGGAAGATATTATAATAATATCTACTGTGAGATCAAATGGAAGTGGTAATATCGGTTTTCTTTCAAATAGACAAAGAGCTAATGTTGCAATGACAAGGGCTAGGTATTCATTGAGTTATTTCTGAATCCTCGTGTGTATTTGCAGATTCAAAGTTGGTTGTAAACTGAGTTCATTTTTTGTGCAGATATTGTCTTTGGATATTAGGCAATGCATCTACTTTAGCCAGTAGTGATTCTATCTGGAGAAAGTTAATTATTGATGCTAAGAGAAGAGACTGTTATCATAATGCTGACGAGGACATGAAACTGGCTCGAGTTATTGATGATGTCTTGCTTGAGATTGAACTTCTTGAAGAATCTGAGTCAACATTTAAGAAACTCAGTCTATGTGAAAAATCTGAAATTGATGCTATACTTCCTCAAG

Protein sequence

>MS.gene81802.t1

IADNCRFGRSMFERLVMLGYKKHMLNVQYRMHPSISLFPCKEFYDEKISDALAVKDESYNKSFLEGEMYSSYSFINISKGEETFGRGHSLKNMVEVAVISEMIKNLRKEFMRTKKKVSIGIISPYNAQVYEIQEKVKQDTWDSNSDFSVNVRSVDGFQGGEEDIIIISTVRSNGSGNIGFLSNRQRANVAMTRARYCLWILGNASTLASSDSIWRKLIIDAKRRDCYHNADEDMKLARVIDDVLLEIELLEESESTFKKLSLCEKSEIDAILPQ