AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene83591

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene83591.t1 MTR_2g104940 98.387 310 5 0 1 310 1 310 0.0 619
MS.gene83591.t1 MTR_4g006730 83.548 310 43 3 4 310 1 305 0.0 506
MS.gene83591.t1 MTR_4g006730 85.124 242 28 3 4 242 1 237 7.06e-142 401
MS.gene83591.t1 MTR_4g006730 87.156 218 28 0 93 310 1 218 6.16e-132 374
MS.gene83591.t1 MTR_4g006630 86.697 218 29 0 93 310 1 218 2.95e-131 372
MS.gene83591.t1 MTR_1g097840 58.224 304 107 4 11 310 13 300 4.38e-110 322
MS.gene83591.t1 MTR_1g097840 58.475 236 82 2 11 242 13 236 4.90e-82 249
MS.gene83591.t1 MTR_8g087710 48.473 262 130 2 45 303 4 263 1.50e-76 235
MS.gene83591.t1 MTR_8g087720 51.542 227 105 2 80 303 38 262 1.41e-74 230
MS.gene83591.t1 MTR_2g017660 51.046 239 113 2 76 310 35 273 2.23e-74 230
MS.gene83591.t1 MTR_2g017590 49.206 252 119 3 56 303 14 260 7.95e-73 226
MS.gene83591.t1 MTR_2g017620 49.206 252 119 3 56 303 2 248 2.97e-72 224
MS.gene83591.t1 MTR_2g017620 49.206 252 119 3 56 303 16 262 3.15e-72 224
MS.gene83591.t1 MTR_4g006650 82.979 141 15 1 81 221 5 136 4.23e-72 226
MS.gene83591.t1 MTR_7g010650 46.121 232 122 1 75 306 32 260 1.92e-71 222
MS.gene83591.t1 MTR_4g084880 44.697 264 134 4 56 310 8 268 2.22e-70 219
MS.gene83591.t1 MTR_2g017570 51.584 221 102 2 76 293 34 252 2.16e-69 217
MS.gene83591.t1 MTR_2g017610 52.535 217 98 2 76 289 34 248 2.80e-69 217
MS.gene83591.t1 MTR_2g017620 54.348 184 77 2 124 303 1 181 2.29e-60 191
MS.gene83591.t1 MTR_5g079570 45.495 222 116 3 76 293 36 256 2.54e-57 186
MS.gene83591.t1 MTR_3g008920 47.685 216 109 3 76 289 22 235 3.03e-55 180
MS.gene83591.t1 MTR_5g063930 43.779 217 116 2 80 292 37 251 5.29e-55 180
MS.gene83591.t1 MTR_8g087720 53.012 166 73 2 80 242 38 201 1.80e-50 166
MS.gene83591.t1 MTR_1g070385 40.175 229 137 0 78 306 60 288 4.43e-48 172
MS.gene83591.t1 MTR_8g087720 50.685 146 67 2 80 222 38 181 6.01e-39 136
MS.gene83591.t1 MTR_7g010650 43.243 148 84 0 75 222 32 179 1.80e-38 135
MS.gene83591.t1 MTR_2g101370 39.286 224 116 8 84 293 24 241 3.18e-32 120
MS.gene83591.t1 MTR_1g006490 37.073 205 109 7 85 277 24 220 6.17e-29 111
MS.gene83591.t1 MTR_8g013680 35.749 207 115 4 84 277 23 224 4.00e-27 106
MS.gene83591.t1 MTR_1g077540 34.404 218 135 3 84 293 25 242 5.51e-26 103
MS.gene83591.t1 MTR_1g006590 36.098 205 111 8 85 277 24 220 6.88e-25 100
MS.gene83591.t1 MTR_6g069040 32.287 223 143 3 84 298 25 247 2.59e-24 99.4
MS.gene83591.t1 MTR_2g094270 31.120 241 132 7 71 278 22 261 2.40e-23 97.4
MS.gene83591.t1 MTR_5g012810 34.783 207 116 4 84 277 23 223 5.03e-23 95.9
MS.gene83591.t1 MTR_3g118010 30.453 243 142 7 73 288 19 261 8.66e-23 95.9
MS.gene83591.t1 MTR_4g063090 33.168 202 127 4 85 278 26 227 1.65e-21 91.7
MS.gene83591.t1 MTR_1g115405 29.075 227 153 3 84 302 25 251 7.33e-21 90.1
MS.gene83591.t1 MTR_2g094270 30.973 226 125 6 71 266 22 246 1.83e-20 89.4
MS.gene83591.t1 MTR_7g101190 28.452 239 140 7 71 278 21 259 1.42e-18 84.3
MS.gene83591.t1 MTR_7g103030 33.010 206 121 6 84 277 25 225 1.91e-18 83.2
MS.gene83591.t1 MTR_3g070210 29.508 244 144 8 73 288 37 280 3.68e-18 83.2
MS.gene83591.t1 MTR_8g098375 29.487 234 137 8 73 278 34 267 1.29e-17 81.6
MS.gene83591.t1 MTR_4g415300 29.717 212 131 7 84 278 60 270 1.80e-16 78.6
MS.gene83591.t1 MTR_5g010150 30.189 212 131 6 84 278 56 267 2.62e-16 78.2
MS.gene83591.t1 MTR_4g059390 27.197 239 141 7 73 278 21 259 3.05e-16 77.8
MS.gene83591.t1 MTR_3g070210 30.093 216 126 7 73 263 37 252 1.21e-15 75.9
MS.gene83591.t1 MTR_7g023350 27.753 227 156 4 84 302 25 251 1.27e-15 75.5
MS.gene83591.t1 MTR_5g082070 28.692 237 135 8 73 278 37 270 3.56e-15 74.7
MS.gene83591.t1 MTR_2g060360 28.571 224 145 6 84 296 23 242 9.48e-14 70.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene83591.t1 AT1G80760 77.564 312 58 4 4 310 1 305 6.48e-167 466
MS.gene83591.t1 AT4G10380 67.416 267 75 3 46 310 48 304 4.94e-119 344
MS.gene83591.t1 AT5G37820 46.484 256 129 4 52 303 16 267 7.96e-72 224
MS.gene83591.t1 AT5G37820 46.484 256 129 4 52 303 52 303 1.20e-71 224
MS.gene83591.t1 AT4G19030 43.686 293 151 5 29 309 2 292 1.51e-70 221
MS.gene83591.t1 AT5G37810 46.964 247 125 3 59 303 25 267 5.68e-70 219
MS.gene83591.t1 AT4G18910 41.472 299 146 4 29 309 2 289 2.78e-69 218
MS.gene83591.t1 AT1G31885 45.600 250 128 2 68 309 31 280 2.52e-66 211
MS.gene83591.t1 AT1G31885 46.903 226 112 2 92 309 1 226 2.85e-61 196
MS.gene83591.t1 AT2G34390 40.217 276 148 5 46 310 13 282 1.79e-54 179
MS.gene83591.t1 AT2G34390 42.083 240 123 4 81 310 7 240 1.49e-51 170
MS.gene83591.t1 AT4G19030 44.000 225 112 5 29 241 2 224 2.54e-49 164
MS.gene83591.t1 AT4G18910 42.241 232 105 4 29 242 2 222 1.99e-48 161
MS.gene83591.t1 AT3G06100 38.208 212 130 1 80 290 46 257 1.45e-43 150
MS.gene83591.t1 AT3G06100 38.389 211 129 1 84 293 2 212 5.97e-43 147
MS.gene83591.t1 AT4G19030 41.748 206 106 5 29 222 2 205 1.49e-38 136
MS.gene83591.t1 AT2G34390 38.942 208 113 4 46 242 13 217 9.03e-35 126
MS.gene83591.t1 AT3G16240 37.198 207 112 5 84 277 23 224 1.14e-29 113
MS.gene83591.t1 AT2G34390 37.368 190 105 4 46 224 13 199 8.63e-28 108
MS.gene83591.t1 AT2G25810 35.885 209 116 7 80 277 19 220 3.33e-26 104
MS.gene83591.t1 AT5G47450 34.783 230 124 7 84 292 23 247 3.69e-26 104
MS.gene83591.t1 AT2G29870 46.957 115 58 1 196 310 22 133 1.10e-25 100
MS.gene83591.t1 AT4G17340 33.816 207 119 4 84 277 23 224 2.58e-24 99.4
MS.gene83591.t1 AT3G26520 34.783 207 117 6 84 277 26 227 7.81e-23 95.5
MS.gene83591.t1 AT2G36830 34.783 207 117 6 84 277 25 226 8.49e-23 95.5
MS.gene83591.t1 AT4G35100 30.864 243 141 7 73 288 20 262 4.05e-22 94.0
MS.gene83591.t1 AT4G35100 30.864 243 141 7 73 288 20 262 4.05e-22 94.0
MS.gene83591.t1 AT2G16850 30.328 244 141 7 73 288 18 260 4.65e-22 94.0
MS.gene83591.t1 AT5G60660 28.689 244 141 7 71 282 21 263 1.70e-20 89.7
MS.gene83591.t1 AT3G06100 34.532 139 90 1 156 293 41 179 7.65e-20 85.9
MS.gene83591.t1 AT3G54820 30.833 240 133 7 71 278 20 258 1.90e-19 87.0
MS.gene83591.t1 AT1G73190 30.493 223 142 4 84 293 28 250 1.41e-18 84.0
MS.gene83591.t1 AT4G01470 30.275 218 134 5 84 288 25 237 2.29e-18 83.2
MS.gene83591.t1 AT1G17810 29.956 227 142 6 84 295 28 252 8.73e-18 82.0
MS.gene83591.t1 AT2G37170 27.308 260 145 8 51 278 10 257 1.34e-17 81.6
MS.gene83591.t1 AT3G53420 27.917 240 140 7 71 278 21 259 1.60e-17 81.6
MS.gene83591.t1 AT3G53420 27.917 240 140 7 71 278 21 259 1.60e-17 81.6
MS.gene83591.t1 AT2G37170 27.308 260 145 8 51 278 53 300 2.97e-17 81.3
MS.gene83591.t1 AT2G45960 31.915 235 129 9 73 278 34 266 8.06e-17 79.3
MS.gene83591.t1 AT2G37180 26.923 260 146 8 51 278 10 257 1.08e-16 79.0
MS.gene83591.t1 AT4G23400 30.189 212 130 6 84 278 57 267 1.31e-16 79.0
MS.gene83591.t1 AT4G00430 29.487 234 136 8 73 278 35 267 1.43e-16 79.0
MS.gene83591.t1 AT1G31885 41.758 91 53 0 66 156 29 119 2.03e-16 75.1
MS.gene83591.t1 AT1G01620 30.660 212 129 6 84 278 56 266 2.41e-16 78.2
MS.gene83591.t1 AT2G39010 28.333 240 137 7 73 278 20 258 2.52e-16 78.2
MS.gene83591.t1 AT3G61430 31.455 213 126 7 84 278 56 266 3.33e-16 77.8
MS.gene83591.t1 AT3G61430 31.455 213 126 7 84 278 56 266 3.33e-16 77.8
MS.gene83591.t1 AT3G47440 27.586 232 151 5 84 303 27 253 2.32e-15 74.7
MS.gene83591.t1 AT1G01620 32.402 179 106 5 114 278 17 194 1.39e-14 72.0
MS.gene83591.t1 AT2G45960 30.594 219 126 7 73 266 34 251 2.07e-14 72.8
MS.gene83591.t1 AT2G45960 31.818 220 122 8 73 266 34 251 2.55e-14 72.0

Find 90 sgRNAs with CRISPR-Local

Find 138 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCATTGACGCTCCTGTTAT+TGG 0.213047 2.2:+1295391 None:intergenic
CATCTTCTAAGTTCCATTCT+TGG 0.216409 2.2:+1296572 None:intergenic
GTGACTCCACCACTCATAAA+AGG 0.246044 2.2:+1295886 None:intergenic
TCTCCGAGCCTTGTGTCTTT+TGG 0.294166 2.2:+1296270 None:intergenic
CTTTGCATTATCATCTTCTT+CGG 0.298809 2.2:+1295246 None:intergenic
CAGATAGGAGCCGAGTTTAT+AGG 0.306620 2.2:-1296338 MS.gene83591:CDS
CCAGGAGTGCCTGGTGTTGC+TGG 0.322227 2.2:+1296691 None:intergenic
CCTGGTGTTGCTGGTGTTGA+TGG 0.322832 2.2:+1296700 None:intergenic
GTATATTGGTGCACAAGTTT+TGG 0.339591 2.2:-1295950 MS.gene83591:CDS
AGATAGGAGCCGAGTTTATA+GGG 0.358525 2.2:-1296337 MS.gene83591:CDS
CTCAAGAATTGTAAATGCTT+TGG 0.371928 2.2:-1296598 MS.gene83591:CDS
GCTGGACCTAGAGTTCTTAC+TGG 0.377381 2.2:+1295367 None:intergenic
CTGCGTTGCAGGCCAATAAC+AGG 0.388557 2.2:-1295403 MS.gene83591:intron
CTCTCCACCGGCCACATCTC+TGG 0.392505 2.2:-1296197 MS.gene83591:CDS
ACCGTTCCTTCCGGAGGATA+TGG 0.393092 2.2:-1295865 MS.gene83591:CDS
TAGTTATTTGCAGCTATAGC+TGG 0.401428 2.2:+1295349 None:intergenic
ATTGGGTGTGCTACCTCTAC+TGG 0.401500 2.2:-1296242 MS.gene83591:CDS
CAAATAACTACAAAGCCATA+TGG 0.404031 2.2:-1295336 MS.gene83591:CDS
ACATTGATTCTGATGTTTGC+TGG 0.409739 2.2:-1296314 MS.gene83591:CDS
CAAGGCTCGGAGACTCTGAT+TGG 0.413496 2.2:-1296260 MS.gene83591:CDS
TTTGCATTATCATCTTCTTC+GGG 0.414167 2.2:+1295247 None:intergenic
TGCAAGAGGCACAGGAGGAG+GGG 0.422658 2.2:+1296513 None:intergenic
CATTGATTCTGATGTTTGCT+GGG 0.434089 2.2:-1296313 MS.gene83591:CDS
CCACCTAGAGCTCCAAGTAT+TGG 0.435294 2.2:+1295298 None:intergenic
GGTGGAGTCACCGTTCCTTC+CGG 0.437337 2.2:-1295874 MS.gene83591:CDS
GTGCTGCATTTGCTCTCAAA+GGG 0.443470 2.2:-1295918 MS.gene83591:CDS
TTTGCAAGAGGCACAGGAGG+AGG 0.446500 2.2:+1296511 None:intergenic
TATCTCTTAGCTCCAATACT+TGG 0.455159 2.2:-1295310 MS.gene83591:CDS
AGTTGATCATCTTCTAAAGC+TGG 0.459544 2.2:+1295201 None:intergenic
TTGCAGGTGCCTATGTATAT+TGG 0.461576 2.2:-1295964 MS.gene83591:intron
CTTCAATGCAGCAAAAGAAA+TGG 0.468532 2.2:+1296151 None:intergenic
ATGCTCAATATACTAATAGC+AGG 0.472157 2.2:-1295522 MS.gene83591:CDS
TTGCAAGAGGCACAGGAGGA+GGG 0.478192 2.2:+1296512 None:intergenic
AGAGGTACACCAGGAGTGCC+TGG 0.482854 2.2:+1296682 None:intergenic
AAATAACTACAAAGCCATAT+GGG 0.491686 2.2:-1295335 MS.gene83591:CDS
CTTGGAGCTCTAGGTGGGGC+AGG 0.496173 2.2:-1295292 MS.gene83591:CDS
GCAAGAGGCACAGGAGGAGG+GGG 0.497170 2.2:+1296514 None:intergenic
GCATTTACAATTCTTGAGTA+GGG 0.497185 2.2:+1296603 None:intergenic
GCAAAAGAAATGGTGACTGC+AGG 0.502542 2.2:+1296161 None:intergenic
CAAGAATGGAACTTAGAAGA+TGG 0.514046 2.2:-1296571 MS.gene83591:CDS
CCAGCAACACCAGGCACTCC+TGG 0.515961 2.2:-1296691 MS.gene83591:CDS
GTTGATCATCTTCTAAAGCT+GGG 0.517734 2.2:+1295202 None:intergenic
AATGGAACATATTAATAATG+AGG 0.519868 2.2:-1296728 MS.gene83591:CDS
ATGAACCCAGTAAGAACTCT+AGG 0.522426 2.2:-1295373 MS.gene83591:CDS
AATGCTTTGGTGTCCAAGAA+TGG 0.524776 2.2:-1296585 MS.gene83591:CDS
TAATCTAATGTTCGTTGTCA+CGG 0.524885 2.2:-1295809 MS.gene83591:CDS
GCTCCAATACTTGGAGCTCT+AGG 0.532633 2.2:-1295301 MS.gene83591:CDS
AGCATTTACAATTCTTGAGT+AGG 0.536216 2.2:+1296602 None:intergenic
TGACTCCACCACTCATAAAA+GGG 0.537986 2.2:+1295887 None:intergenic
GCTTTGGTTACATGCTTCCA+CGG 0.548483 2.2:+1296122 None:intergenic
TGTGCTGCATTTGCTCTCAA+AGG 0.550088 2.2:-1295919 MS.gene83591:CDS
AAGGCTCGGAGACTCTGATT+GGG 0.552417 2.2:-1296259 MS.gene83591:CDS
GGTTTAAGTCTGAGAGAAGC+GGG 0.553612 2.2:-1296651 MS.gene83591:CDS
AATGTTCGTTGTCACGGCAG+TGG 0.554743 2.2:-1295803 MS.gene83591:CDS
AAGTCTGAGAGAAGCGGGAA+TGG 0.555069 2.2:-1296646 MS.gene83591:CDS
CAGAGATGTGGCCGGTGGAG+AGG 0.557528 2.2:+1296198 None:intergenic
CTGGACCTAGAGTTCTTACT+GGG 0.557635 2.2:+1295368 None:intergenic
TGCTACCTCTACTGGTCTTG+CGG 0.560160 2.2:-1296234 MS.gene83591:CDS
CAATACTTGGAGCTCTAGGT+GGG 0.562103 2.2:-1295297 MS.gene83591:CDS
GAACTAGCAGGAATTGCAGT+GGG 0.569469 2.2:-1295555 MS.gene83591:CDS
CCAATACTTGGAGCTCTAGG+TGG 0.572700 2.2:-1295298 MS.gene83591:CDS
ACCATATCCTCCGGAAGGAA+CGG 0.576185 2.2:+1295864 None:intergenic
AGCTAAGAGATAGACCCATA+TGG 0.576739 2.2:+1295321 None:intergenic
CCATCAACACCAGCAACACC+AGG 0.577155 2.2:-1296700 MS.gene83591:CDS
CTGCATTGAAGCACTTCCCG+TGG 0.578067 2.2:-1296139 MS.gene83591:CDS
CATAACCGCAAGACCAGTAG+AGG 0.586587 2.2:+1296229 None:intergenic
CATTAATGAGCAGGTAACTG+TGG 0.587885 2.2:+1296540 None:intergenic
ATGATCATCATCCTCTCCAC+CGG 0.588983 2.2:-1296209 MS.gene83591:CDS
CAAAAGAAATGGTGACTGCA+GGG 0.592708 2.2:+1296162 None:intergenic
AAAAGCTTGACCATATCCTC+CGG 0.599263 2.2:+1295855 None:intergenic
ACTTGTGCACCAATATACAT+AGG 0.600697 2.2:+1295955 None:intergenic
AATCAATGTCCCTATAAACT+CGG 0.603006 2.2:+1296328 None:intergenic
CTTTGGTTACATGCTTCCAC+GGG 0.610743 2.2:+1296123 None:intergenic
AGCACCAGAGATGTGGCCGG+TGG 0.615438 2.2:+1296193 None:intergenic
TGAGATGAGCACCAGAGATG+TGG 0.617163 2.2:+1296186 None:intergenic
TGCTGCATTTGCTCTCAAAG+GGG 0.620449 2.2:-1295917 MS.gene83591:CDS
GGAGTCACCGTTCCTTCCGG+AGG 0.622617 2.2:-1295871 MS.gene83591:CDS
ACTCACAGCTCTTGTGTCGG+TGG 0.623869 2.2:+1295780 None:intergenic
GGGTTTAAGTCTGAGAGAAG+CGG 0.625558 2.2:-1296652 MS.gene83591:CDS
ATGTAGGTAGGAGAACTAGC+AGG 0.625936 2.2:-1295567 MS.gene83591:intron
ATTAATGAGCAGGTAACTGT+GGG 0.626119 2.2:+1296541 None:intergenic
AGAACTAGCAGGAATTGCAG+TGG 0.628860 2.2:-1295556 MS.gene83591:CDS
TTGATCATCTTCTAAAGCTG+GGG 0.642052 2.2:+1295203 None:intergenic
GAACCAAAAGACACAAGGCT+CGG 0.650309 2.2:-1296273 MS.gene83591:CDS
GCTTGACCATATCCTCCGGA+AGG 0.651188 2.2:+1295859 None:intergenic
AATACTTGGAGCTCTAGGTG+GGG 0.656773 2.2:-1295296 MS.gene83591:CDS
TGCTCAATATACTAATAGCA+GGG 0.657754 2.2:-1295521 MS.gene83591:intron
AATACTCACAGCTCTTGTGT+CGG 0.671145 2.2:+1295777 None:intergenic
ATGAGCACCAGAGATGTGGC+CGG 0.675762 2.2:+1296190 None:intergenic
ATTGTGAACCAAAAGACACA+AGG 0.703696 2.2:-1296278 MS.gene83591:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAGTATAAATTATAATTGC+AGG - chr2.2:1295954-1295973 MS.gene83591:CDS 15.0%
!! TAATTTCAACAACTTTAATT+AGG + chr2.2:1295864-1295883 None:intergenic 15.0%
!!! CATATGTTCAAATTTTAATT+TGG + chr2.2:1296282-1296301 None:intergenic 15.0%
!! ACAACTTTAATTAGGAAATT+AGG + chr2.2:1295856-1295875 None:intergenic 20.0%
!! ATAACCATAAACATCAATTA+GGG + chr2.2:1296349-1296368 None:intergenic 20.0%
!! TATTAGCTAAAAGTTACTAT+AGG + chr2.2:1296187-1296206 None:intergenic 20.0%
!! TTAAAATTTGAACATATGCT+TGG - chr2.2:1296284-1296303 MS.gene83591:CDS 20.0%
! AAATAACTACAAAGCCATAT+GGG - chr2.2:1296599-1296618 MS.gene83591:CDS 25.0%
! AGTTTGAAAAGAAAAAGAGA+AGG - chr2.2:1296225-1296244 MS.gene83591:CDS 25.0%
! ATATATATGTTCATGACAAG+TGG + chr2.2:1296502-1296521 None:intergenic 25.0%
! ATTGATGTTTATGGTTATGT+AGG - chr2.2:1296351-1296370 MS.gene83591:intron 25.0%
! ATTTATGTGTGTCTAATCTT+TGG - chr2.2:1295496-1295515 MS.gene83591:intron 25.0%
! CATAACCATAAACATCAATT+AGG + chr2.2:1296350-1296369 None:intergenic 25.0%
! TATATATGTTCATGACAAGT+GGG + chr2.2:1296501-1296520 None:intergenic 25.0%
! TGTCATGAACATATATATGA+TGG - chr2.2:1296504-1296523 MS.gene83591:intron 25.0%
!!! ATATATACCTTTTTTGCAAG+AGG + chr2.2:1295438-1295457 None:intergenic 25.0%
AATCAATGTCCCTATAAACT+CGG + chr2.2:1295609-1295628 None:intergenic 30.0%
AGCATTTACAATTCTTGAGT+AGG + chr2.2:1295335-1295354 None:intergenic 30.0%
ATATATGTTCATGACAAGTG+GGG + chr2.2:1296500-1296519 None:intergenic 30.0%
ATGCTCAATATACTAATAGC+AGG - chr2.2:1296412-1296431 MS.gene83591:intron 30.0%
ATGTTTATGGTTATGTAGGT+AGG - chr2.2:1296355-1296374 MS.gene83591:intron 30.0%
CAAATAACTACAAAGCCATA+TGG - chr2.2:1296598-1296617 MS.gene83591:CDS 30.0%
CTCAAGAATTGTAAATGCTT+TGG - chr2.2:1295336-1295355 MS.gene83591:CDS 30.0%
CTTTGCATTATCATCTTCTT+CGG + chr2.2:1296691-1296710 None:intergenic 30.0%
GCATTTACAATTCTTGAGTA+GGG + chr2.2:1295334-1295353 None:intergenic 30.0%
TAATCTAATGTTCGTTGTCA+CGG - chr2.2:1296125-1296144 MS.gene83591:intron 30.0%
TAATTAGGAAATTAGGCTGA+AGG + chr2.2:1295849-1295868 None:intergenic 30.0%
TACTTAGCACTTCAATGATA+TGG + chr2.2:1295542-1295561 None:intergenic 30.0%
TGCTCAATATACTAATAGCA+GGG - chr2.2:1296413-1296432 MS.gene83591:intron 30.0%
TTTGCATTATCATCTTCTTC+GGG + chr2.2:1296690-1296709 None:intergenic 30.0%
AAACTACCTGTGTTACAGAT+AGG - chr2.2:1295581-1295600 MS.gene83591:intron 35.0%
ACTTGTGCACCAATATACAT+AGG + chr2.2:1295982-1296001 None:intergenic 35.0%
ATTAATGAGCAGGTAACTGT+GGG + chr2.2:1295396-1295415 None:intergenic 35.0%
ATTGTGAACCAAAAGACACA+AGG - chr2.2:1295656-1295675 MS.gene83591:intron 35.0%
CAAGAATGGAACTTAGAAGA+TGG - chr2.2:1295363-1295382 MS.gene83591:CDS 35.0%
CATCTTCTAAGTTCCATTCT+TGG + chr2.2:1295365-1295384 None:intergenic 35.0%
CTTCAATGCAGCAAAAGAAA+TGG + chr2.2:1295786-1295805 None:intergenic 35.0%
GAAATTAGGCTGAAGGATTA+AGG + chr2.2:1295842-1295861 None:intergenic 35.0%
GAAGGATTAAGGAAAAGCTT+TGG + chr2.2:1295831-1295850 None:intergenic 35.0%
GAGTCCCTAATTGATGTTTA+TGG - chr2.2:1296342-1296361 MS.gene83591:intron 35.0%
GATGATATTATCCCCTATGT+CGG + chr2.2:1296260-1296279 None:intergenic 35.0%
TAGTTATTTGCAGCTATAGC+TGG + chr2.2:1296588-1296607 None:intergenic 35.0%
TATCTCTTAGCTCCAATACT+TGG - chr2.2:1296624-1296643 MS.gene83591:CDS 35.0%
! ATCTGTAACACAGGTAGTTT+TGG + chr2.2:1295581-1295600 None:intergenic 35.0%
! GTATATTGGTGCACAAGTTT+TGG - chr2.2:1295984-1296003 MS.gene83591:intron 35.0%
! GTTTTTCACCCTTTTATGAG+TGG - chr2.2:1296039-1296058 MS.gene83591:intron 35.0%
!! ACATTGATTCTGATGTTTGC+TGG - chr2.2:1295620-1295639 MS.gene83591:intron 35.0%
!! CATTGATTCTGATGTTTGCT+GGG - chr2.2:1295621-1295640 MS.gene83591:intron 35.0%
AAAAGCTTGACCATATCCTC+CGG + chr2.2:1296082-1296101 None:intergenic 40.0%
AATACTCACAGCTCTTGTGT+CGG + chr2.2:1296160-1296179 None:intergenic 40.0%
AGATAGGAGCCGAGTTTATA+GGG - chr2.2:1295597-1295616 MS.gene83591:intron 40.0%
AGCTAAGAGATAGACCCATA+TGG + chr2.2:1296616-1296635 None:intergenic 40.0%
ATGAACCCAGTAAGAACTCT+AGG - chr2.2:1296561-1296580 MS.gene83591:CDS 40.0%
CAAAAGAAATGGTGACTGCA+GGG + chr2.2:1295775-1295794 None:intergenic 40.0%
CATTAATGAGCAGGTAACTG+TGG + chr2.2:1295397-1295416 None:intergenic 40.0%
GACTTAAACCCACCAAAAAG+AGG + chr2.2:1295273-1295292 None:intergenic 40.0%
GAGAGTGATAAGTGAGAGTT+AGG + chr2.2:1296450-1296469 None:intergenic 40.0%
TGACTCCACCACTCATAAAA+GGG + chr2.2:1296050-1296069 None:intergenic 40.0%
TTAATTGCTCTTCACGCGAA+GGG + chr2.2:1296320-1296339 None:intergenic 40.0%
TTCATTGACGCTCCTGTTAT+TGG + chr2.2:1296546-1296565 None:intergenic 40.0%
TTGCAGGTGCCTATGTATAT+TGG - chr2.2:1295970-1295989 MS.gene83591:intron 40.0%
! TTTCACCCTTTTATGAGTGG+TGG - chr2.2:1296042-1296061 MS.gene83591:intron 40.0%
!! AATGCTTTGGTGTCCAAGAA+TGG - chr2.2:1295349-1295368 MS.gene83591:CDS 40.0%
!!! CTTTTGGTTCACAATAGCAG+TGG + chr2.2:1295651-1295670 None:intergenic 40.0%
AAGGAAGATGAAGCCGACAT+AGG - chr2.2:1296244-1296263 MS.gene83591:CDS 45.0%
AGAACTAGCAGGAATTGCAG+TGG - chr2.2:1296378-1296397 MS.gene83591:intron 45.0%
AGGAAGATGAAGCCGACATA+GGG - chr2.2:1296245-1296264 MS.gene83591:CDS 45.0%
ATGATCATCATCCTCTCCAC+CGG - chr2.2:1295725-1295744 MS.gene83591:intron 45.0%
ATGTAGGTAGGAGAACTAGC+AGG - chr2.2:1296367-1296386 MS.gene83591:intron 45.0%
CAGATAGGAGCCGAGTTTAT+AGG - chr2.2:1295596-1295615 MS.gene83591:intron 45.0%
CTGGACCTAGAGTTCTTACT+GGG + chr2.2:1296569-1296588 None:intergenic 45.0%
CTTTGGTTACATGCTTCCAC+GGG + chr2.2:1295814-1295833 None:intergenic 45.0%
GAACCAAAAGACACAAGGCT+CGG - chr2.2:1295661-1295680 MS.gene83591:intron 45.0%
GAACTAGCAGGAATTGCAGT+GGG - chr2.2:1296379-1296398 MS.gene83591:intron 45.0%
GCAAAAGAAATGGTGACTGC+AGG + chr2.2:1295776-1295795 None:intergenic 45.0%
GCTTTGGTTACATGCTTCCA+CGG + chr2.2:1295815-1295834 None:intergenic 45.0%
GGGTTTAAGTCTGAGAGAAG+CGG - chr2.2:1295282-1295301 MS.gene83591:CDS 45.0%
GGTTTAAGTCTGAGAGAAGC+GGG - chr2.2:1295283-1295302 MS.gene83591:CDS 45.0%
GTGACTCCACCACTCATAAA+AGG + chr2.2:1296051-1296070 None:intergenic 45.0%
GTGCTGCATTTGCTCTCAAA+GGG - chr2.2:1296016-1296035 MS.gene83591:intron 45.0%
GTTAATTGCTCTTCACGCGA+AGG + chr2.2:1296321-1296340 None:intergenic 45.0%
GTTCATGACAAGTGGGGATA+GGG + chr2.2:1296494-1296513 None:intergenic 45.0%
TAATTGCTCTTCACGCGAAG+GGG + chr2.2:1296319-1296338 None:intergenic 45.0%
TCCTGTGCCTCTTGCAAAAA+AGG - chr2.2:1295428-1295447 MS.gene83591:intron 45.0%
TGCTGCATTTGCTCTCAAAG+GGG - chr2.2:1296017-1296036 MS.gene83591:intron 45.0%
TGTGCTGCATTTGCTCTCAA+AGG - chr2.2:1296015-1296034 MS.gene83591:intron 45.0%
TGTTCATGACAAGTGGGGAT+AGG + chr2.2:1296495-1296514 None:intergenic 45.0%
TTCATGACAAGTGGGGATAG+GGG + chr2.2:1296493-1296512 None:intergenic 45.0%
! ACTCCTGGTGTACCTCTTTT+TGG - chr2.2:1295258-1295277 MS.gene83591:CDS 45.0%
!! AATACTTGGAGCTCTAGGTG+GGG - chr2.2:1296638-1296657 MS.gene83591:CDS 45.0%
!! CAATACTTGGAGCTCTAGGT+GGG - chr2.2:1296637-1296656 MS.gene83591:CDS 45.0%
!! CTGGTGTACCTCTTTTTGGT+GGG - chr2.2:1295262-1295281 MS.gene83591:CDS 45.0%
!!! ACCTTTTTTGCAAGAGGCAC+AGG + chr2.2:1295432-1295451 None:intergenic 45.0%
!!! TTTTTTGCAAGAGGCACAGG+AGG + chr2.2:1295429-1295448 None:intergenic 45.0%
AAGGCTCGGAGACTCTGATT+GGG - chr2.2:1295675-1295694 MS.gene83591:intron 50.0%
AAGTCTGAGAGAAGCGGGAA+TGG - chr2.2:1295288-1295307 MS.gene83591:CDS 50.0%
AATGTTCGTTGTCACGGCAG+TGG - chr2.2:1296131-1296150 MS.gene83591:intron 50.0%
ACCATATCCTCCGGAAGGAA+CGG + chr2.2:1296073-1296092 None:intergenic 50.0%
ATTGGGTGTGCTACCTCTAC+TGG - chr2.2:1295692-1295711 MS.gene83591:intron 50.0%
CATAACCGCAAGACCAGTAG+AGG + chr2.2:1295708-1295727 None:intergenic 50.0%
CCACCTAGAGCTCCAAGTAT+TGG + chr2.2:1296639-1296658 None:intergenic 50.0%
GCTCCAATACTTGGAGCTCT+AGG - chr2.2:1296633-1296652 MS.gene83591:CDS 50.0%
GCTGGACCTAGAGTTCTTAC+TGG + chr2.2:1296570-1296589 None:intergenic 50.0%
GGAAGATGAAGCCGACATAG+GGG - chr2.2:1296246-1296265 MS.gene83591:CDS 50.0%
TCATGACAAGTGGGGATAGG+GGG + chr2.2:1296492-1296511 None:intergenic 50.0%
TCGGCTCCTATCTGTAACAC+AGG + chr2.2:1295590-1295609 None:intergenic 50.0%
TGAGATGAGCACCAGAGATG+TGG + chr2.2:1295751-1295770 None:intergenic 50.0%
! CCACCAAAAAGAGGTACACC+AGG + chr2.2:1295264-1295283 None:intergenic 50.0%
! CCTGGTGTACCTCTTTTTGG+TGG - chr2.2:1295261-1295280 MS.gene83591:CDS 50.0%
!! CCAATACTTGGAGCTCTAGG+TGG - chr2.2:1296636-1296655 MS.gene83591:CDS 50.0%
!! TCTCCGAGCCTTGTGTCTTT+TGG + chr2.2:1295667-1295686 None:intergenic 50.0%
!! TGCTACCTCTACTGGTCTTG+CGG - chr2.2:1295700-1295719 MS.gene83591:intron 50.0%
AAGTGGGGATAGGGGGAAGA+AGG + chr2.2:1296485-1296504 None:intergenic 55.0%
ACCGTTCCTTCCGGAGGATA+TGG - chr2.2:1296069-1296088 MS.gene83591:intron 55.0%
ACTCACAGCTCTTGTGTCGG+TGG + chr2.2:1296157-1296176 None:intergenic 55.0%
AGGGGGTGGCATTAATGAGC+AGG + chr2.2:1295406-1295425 None:intergenic 55.0%
ATGAGCACCAGAGATGTGGC+CGG + chr2.2:1295747-1295766 None:intergenic 55.0%
CAAGGCTCGGAGACTCTGAT+TGG - chr2.2:1295674-1295693 MS.gene83591:intron 55.0%
CCATCAACACCAGCAACACC+AGG - chr2.2:1295234-1295253 MS.gene83591:CDS 55.0%
CTGCGTTGCAGGCCAATAAC+AGG - chr2.2:1296531-1296550 MS.gene83591:CDS 55.0%
GCTTGACCATATCCTCCGGA+AGG + chr2.2:1296078-1296097 None:intergenic 55.0%
! ATGATGGTGCACTGCGTTGC+AGG - chr2.2:1296520-1296539 MS.gene83591:CDS 55.0%
! CTGCATTGAAGCACTTCCCG+TGG - chr2.2:1295795-1295814 MS.gene83591:CDS 55.0%
! TTGCAAGAGGCACAGGAGGA+GGG + chr2.2:1295425-1295444 None:intergenic 55.0%
! TTTGCAAGAGGCACAGGAGG+AGG + chr2.2:1295426-1295445 None:intergenic 55.0%
!! CCTGGTGTTGCTGGTGTTGA+TGG + chr2.2:1295237-1295256 None:intergenic 55.0%
GGTGGAGTCACCGTTCCTTC+CGG - chr2.2:1296060-1296079 MS.gene83591:intron 60.0%
! TGCAAGAGGCACAGGAGGAG+GGG + chr2.2:1295424-1295443 None:intergenic 60.0%
!! AGAGGTACACCAGGAGTGCC+TGG + chr2.2:1295255-1295274 None:intergenic 60.0%
AGCACCAGAGATGTGGCCGG+TGG + chr2.2:1295744-1295763 None:intergenic 65.0%
CAGAGATGTGGCCGGTGGAG+AGG + chr2.2:1295739-1295758 None:intergenic 65.0%
CCAGCAACACCAGGCACTCC+TGG - chr2.2:1295243-1295262 MS.gene83591:CDS 65.0%
CTCTCCACCGGCCACATCTC+TGG - chr2.2:1295737-1295756 MS.gene83591:intron 65.0%
GGAGTCACCGTTCCTTCCGG+AGG - chr2.2:1296063-1296082 MS.gene83591:intron 65.0%
! GCAAGAGGCACAGGAGGAGG+GGG + chr2.2:1295423-1295442 None:intergenic 65.0%
!! CCAGGAGTGCCTGGTGTTGC+TGG + chr2.2:1295246-1295265 None:intergenic 65.0%
!! CTTGGAGCTCTAGGTGGGGC+AGG - chr2.2:1296642-1296661 MS.gene83591:CDS 65.0%
! AGAGGCACAGGAGGAGGGGG+TGG + chr2.2:1295420-1295439 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 1295207 1296749 1295207 ID=MS.gene83591
chr2.2 mRNA 1295207 1296749 1295207 ID=MS.gene83591.t1;Parent=MS.gene83591
chr2.2 exon 1296507 1296749 1296507 ID=MS.gene83591.t1.exon1;Parent=MS.gene83591.t1
chr2.2 CDS 1296507 1296749 1296507 ID=cds.MS.gene83591.t1;Parent=MS.gene83591.t1
chr2.2 exon 1296133 1296357 1296133 ID=MS.gene83591.t1.exon2;Parent=MS.gene83591.t1
chr2.2 CDS 1296133 1296357 1296133 ID=cds.MS.gene83591.t1;Parent=MS.gene83591.t1
chr2.2 exon 1295786 1295980 1295786 ID=MS.gene83591.t1.exon3;Parent=MS.gene83591.t1
chr2.2 CDS 1295786 1295980 1295786 ID=cds.MS.gene83591.t1;Parent=MS.gene83591.t1
chr2.2 exon 1295522 1295583 1295522 ID=MS.gene83591.t1.exon4;Parent=MS.gene83591.t1
chr2.2 CDS 1295522 1295583 1295522 ID=cds.MS.gene83591.t1;Parent=MS.gene83591.t1
chr2.2 exon 1295207 1295414 1295207 ID=MS.gene83591.t1.exon5;Parent=MS.gene83591.t1
chr2.2 CDS 1295207 1295414 1295207 ID=cds.MS.gene83591.t1;Parent=MS.gene83591.t1
Gene Sequence

>MS.gene83591

ATGGAACATATTAATAATGAGGAAATTCCATCAACACCAGCAACACCAGGCACTCCTGGTGTACCTCTTTTTGGTGGGTTTAAGTCTGAGAGAAGCGGGAATGGTAATAATAAATATAAGAAGTCCCTACTCAAGAATTGTAAATGCTTTGGTGTCCAAGAATGGAACTTAGAAGATGGAGCTCTACCCACAGTTACCTGCTCATTAATGCCACCCCCTCCTCCTGTGCCTCTTGCAAAAAAGGTATATATTACTTTTATTATTATATACATATAATACAACTATGTATATTTATGTGTGTCTAATCTTTGGTCTAATGTGATCAATGTAGACCATATCATTGAAGTGCTAAGTAGTAGTAATTGATTAATCCAAAACTACCTGTGTTACAGATAGGAGCCGAGTTTATAGGGACATTGATTCTGATGTTTGCTGGGACAGCCACTGCTATTGTGAACCAAAAGACACAAGGCTCGGAGACTCTGATTGGGTGTGCTACCTCTACTGGTCTTGCGGTTATGATCATCATCCTCTCCACCGGCCACATCTCTGGTGCTCATCTCAACCCTGCAGTCACCATTTCTTTTGCTGCATTGAAGCACTTCCCGTGGAAGCATGTAACCAAAGCTTTTCCTTAATCCTTCAGCCTAATTTCCTAATTAAAGTTGTTGAAATTAACATGAATTAATTAAAGCTGTACTTAATTTGCATGTTTAATAAATTATAAGCATGCATGTGTGTAAATATAAAGTATAAATTATAATTGCAGGTGCCTATGTATATTGGTGCACAAGTTTTGGCATCAATATGTGCTGCATTTGCTCTCAAAGGGGTTTTTCACCCTTTTATGAGTGGTGGAGTCACCGTTCCTTCCGGAGGATATGGTCAAGCTTTTGCTTTAGAATTCATTATCAGCTTTAATCTAATGTTCGTTGTCACGGCAGTGGCCACCGACACAAGAGCTGTGAGTATTTGTTCCTATAGTAACTTTTAGCTAATAAACACACAAACTCATTCAAGTTTGAAAAGAAAAAGAGAAGGAAGATGAAGCCGACATAGGGGATAATATCATCCAAATTAAAATTTGAACATATGCTTGGATGAAAAATCCCCTTCGCGTGAAGAGCAATTAACAGAGTCCCTAATTGATGTTTATGGTTATGTAGGTAGGAGAACTAGCAGGAATTGCAGTGGGAGCAACTGTGATGCTCAATATACTAATAGCAGGGTATGTCTATGTCCTAACTCTCACTTATCACTCTCAACATACTCTCTCCTTCTTCCCCCTATCCCCACTTGTCATGAACATATATATGATGGTGCACTGCGTTGCAGGCCAATAACAGGAGCGTCAATGAACCCAGTAAGAACTCTAGGTCCAGCTATAGCTGCAAATAACTACAAAGCCATATGGGTCTATCTCTTAGCTCCAATACTTGGAGCTCTAGGTGGGGCAGGAACTTATACTGCAGTCAAGCTTCCCGAAGAAGATGATAATGCAAAGACTAATGCTTCTTCAAATCACCCCAGCTTTAGAAGATGA
Protein sequence

>MS.gene83591.t1

MEHINNEEIPSTPATPGTPGVPLFGGFKSERSGNGNNKYKKSLLKNCKCFGVQEWNLEDGALPTVTCSLMPPPPPVPLAKKIGAEFIGTLILMFAGTATAIVNQKTQGSETLIGCATSTGLAVMIIILSTGHISGAHLNPAVTISFAALKHFPWKHVPMYIGAQVLASICAAFALKGVFHPFMSGGVTVPSGGYGQAFALEFIISFNLMFVVTAVATDTRAVGELAGIAVGATVMLNILIAGPITGASMNPVRTLGPAIAANNYKAIWVYLLAPILGALGGAGTYTAVKLPEEDDNAKTNASSNHPSFRR